The invention discloses a mite-killing composition containing etoxazole and bifenazate, wherein the weight ratio of the etoxazole to the bifenazate is (30:1)-(1:60), is preferably (10:1)-(1:25) and is more preferably (3:1)-(1:16). The composition can be prepared into a water dispersing particle, a suspension, wettable powder, a dispersible oil suspension, missible oil, an emulsion in water or a microemulsion. The mite-killing composition has reasonable component, good mite-killing effect and low cost of used medicines, achieves the activity and the mite-killing effect not by the simple superposition of all the components, and has obvious synergism. The mite-killing composition takes effect quickly, has long effect-holding period, delays the resistance production, is safe to crops, meets the safety requirement of a pesticide preparation, and has better effect of preventing and treating citrus tree, apple tree and cotton red spiders.