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69 results about "Amitraz" patented technology

Amitraz (development code BTS27419) is a non-systemic acaricide and insecticide and has also been described as a scabicide. It was first synthesized by the Boots Co. in England in 1969. Amitraz has been found to have an insect repellent effect, works as an insecticide and also as a pesticide synergist. Its effectiveness is traced back on alpha-adrenergic agonist activity, interaction with octopamine receptors of the central nervous system and inhibition of monoamine oxidases and prostaglandin synthesis. Therefore, it leads to overexcitation and consequently paralysis and death in insects. Because amitraz is less harmful to mammals, amitraz is among many other purposes best known as insecticide against mite- or tick-infestation of dogs. It is also widely used in the beekeeping industry as a control for the Varroa destructor mite, although there are recent reports of resistance (driven by overuse and off label use)reference needed here, questionable statement.

Method for measuring residual quantities of amitraz in royal jelly and metabolites of amitraz by liquid chromatography-mass spectrography/mass spectrometry

The invention relates to a detecting method for measuring residual quantities of amitraz in royal jelly and metabolites of the amitraz, in particular to a method for measuring the amitraz, N-(2,4-dimethylphenyl)-N'-methylfomamidine, N-(2,4-dimethylphenyl) formamide and 2,4-dimethylaniline in the royal jelly by liquid chromatography-mass spectrography/mass spectrometry. The method for measuring the residual quantities of the amitraz in the royal jelly and the metabolites of the amitraz by the liquid chromatography-mass spectrography/mass spectrometry comprises the following steps: adding ammoniation water in a test specimen for diluting; then precipitating protein by using ammoniac acetonitrile and extracting the protein; purifying an extracting solution by using a neutral alumina solid phase extraction column; adopting a liquid chromatography-mass spectrography/mass spectrometer for detecting; quantifying the amitraz and the 2,4-dimethylaniline by an internal standard method; quantifying the N-(2,4-dimethylphenyl)-N'-methylfomamidine and the N-(2,4-dimethylphenyl) formamide by an external standard method. The sensitivity of the method can meet detection and verification requirements of the residual quantities of the amitraz in the royal jelly and the metabolites of the amitraz.
Owner:ZHEJIANG ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE

Method for detecting content of amitraz

A method for detecting the content of amitraz includes the following steps that firstly, an amitraz standard substance is taken, standard solutions of a series of concentrations are prepared, detection is carried out with the high performance liquid chromatography, high performance liquid chromatograms of the standard solutions of the series of concentrations are obtained, and an amitraz standard curve is obtained; secondly, a sample to be detected is taken, a sample solution is prepared, detection is carried out with the high performance liquid chromatography, and a high performance liquid chromatogram of the sample solution is obtained; thirdly, the content of amitraz in the sample to be detected is calculated with the external standard method according to the obtained high performance liquid chromatogram of the sample solution and the obtained amitraz standard curve. According to the chromatographic conditions of the high performance liquid chromatography adopted in the first step and the second step, octadecylsilane chemically bonded silica serves as a packed column, an acetonitrile-phosphoric acid solution serves as a mobile phase, the flow velocity of the mobile phase is 0.5-1.5 ml / min, the column temperature is 20-40 DEG C, and the detection wavelength is 250-350 nm. By the adoption of the high performance liquid chromatography, the method for detecting the content of amitraz is easy, convenient and fast to implement, high in university, short in peak appearance time, capable of saving time and labor, economical and environmentally friendly.
Owner:FOSHAN NANHAI EASTERN ALONG PHARMA CO LTD

Compound oil-in-water cnidium fruit oil nanoemulsion composition and preparation method thereof

The invention discloses a compound oil-in-water cnidium fruit oil nanoemulsion composition. The particle size of the nanoemulsion composition is between 1 nm and 100nm. The nanoemulsion composition comprises the following raw materials in percentage by mass: 0.1 to 9.0 percent of cnidium fruit oil, 0.1 to 3.5 percent of eugenol, 0.1 to 2.0 percent of amitraz, 20.0 to 35.0 percent of surfactant, 0.1 to 6.0 percent of cosurfactant and the balance of distilled water, wherein the total percentage by weight of the raw materials is 100 percent. The compound cnidium fruit oil nanoemulsion has the effects of killing pests, relieving itching and killing bacteria and can be applied to the treatment of parasites (such as sarcoptic mites, psoroptidae, ticks, lice and the like) on body surfaces of animals. Through the compound oil-in-water cnidium fruit oil nanoemulsion composition, the stability and the bioavailability of the medicament are improved, the dosage of the medicament is reduced, the permeability is high, the insecticidal range is wide, the effect is obvious, and the preparation method is simple. Therefore, the compound oil-in-water cnidium fruit oil nanoemulsion composition is convenient to popularize and has wide market prospect in the field of medicaments.
Owner:NORTHWEST A & F UNIV

Method for simultaneously determining residual quantities of amitraz and metabolites in royal jelly through dispersive solid phase extraction-liquid chromatography-mass spectrometry/mass spectrometry method

The invention relates to a method for determining residual quantities of amitraz and three metabolites thereof in royal jelly, in particular to a method for simultaneously determining the residual quantities of the amitraz and the metabolites in royal jelly through a dispersive solid phase extraction-liquid chromatography-mass spectrometry / mass spectrometry method. The method includes the steps that after a buffer solution with pH being 9 is added into a sample to conduct diluting, and protein precipitation, extraction, and salt precipitation are carried out through acetonitrile and sodium chloride; dispersive solid phase extraction is conducted through an adsorbent containing N-propyl ethylenediamine (PSA), anhydrous magnesium sulfate and C18 powder to purify an extraction solution, detection is carried out through a liquid chromatography-mass spectrometry / mass spectrometer, and quantifying is carried out through an isotope internal standard dilution method. The method is environmentally friendly, easy, convenient and quick, small in resource consumption, and low in detection cost, and the sensitivity of the method can meet the requirements of detection and confirmation of the residual quantities of the amitraz and the metabolites thereof in the royal jelly.
Owner:ZHEJIANG ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE

Preparation method and application of amitraz molecular imprinting monolithic column

The invention discloses a preparation method and application of an amitraz molecular imprinting monolithic column. The preparation method comprises the following steps: by taking amitraz as a template molecule, dissolving the template molecule into a mixed solution of acetonitrile and dodecyl alcohol, adding a functional monomer, pre-polymerizing at a low temperature, further adding a cross-linking agent and an initiator, introducing nitrogen, filling into a chromatographic column hollow tube of which the lower end is already sealed, sealing the upper end of the chromatographic column hollow tube, and initializing polymerization, thereby obtaining the molecular imprinting monolithic column. After the functional monomer, a pore-foaming agent and the template molecule which are not polymerized are washed off from the column by using a mixed solution of methanol and acetic acid, the molecular imprinting monolithic column which has a good adsorption effect and an enrichment effect on amitraz is obtained. Compared with a conventional body polymerization method, the preparation method has the characteristics that the preparation method is simple and convenient, the aftertreatment is simple, the recognition property is good, the cost is low, required instruments and reaction conditions are easy to achieve, and the like. The molecular imprinting monolithic column prepared by using the preparation method is capable of separating and detecting residual amitraz in foods.
Owner:HENAN UNIV OF SCI & TECH
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