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Method for detecting content of amitraz

A detection method, the technology of amitraz, applied in the field of analytical chemistry detection, can solve the problems of poor reproducibility of detection results, large use ratio, high equipment requirements, etc., achieve convenient and fast sample processing, reduce the use ratio, and improve detection sensitivity high effect

Inactive Publication Date: 2016-11-09
FOSHAN NANHAI EASTERN ALONG PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Gas chromatography needs to derivatize amitraz, and the sample processing process is cumbersome; in traditional liquid chromatography, organic solvents are used in a large proportion, and the noise is large, the baseline is not stable, and the reproducibility of test results is not good; gas chromatography Chromatography-mass spectrometry requires high equipment and is expensive, which is unaffordable for ordinary laboratories; imprinted columns are suitable for selective separation of target objects, but have poor versatility, and the swelling and shrinking effect of polymers in organic solvents will reduce the stability of polymers properties, and the pore size distribution range of the polymer monolithic medium is very wide, and the column efficiency is low; therefore, it is imperative to establish a fast and efficient analytical method for amitraz

Method used

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  • Method for detecting content of amitraz

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Experimental program
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Effect test

Embodiment 1

[0041] A detection method for amitraz, comprising the following steps:

[0042] A. Take amitraz standard substance, dissolve it with N,N-dimethylformamide, and filter at constant volume to prepare 0.07mg / ml, 0.08mg / ml, 0.09mg / ml, 0.1mg / ml, 0.2mg / ml ml, 0.3mg / ml, 0.4mg / ml series concentration standard solution, use high performance liquid chromatography to detect, obtain series concentration standard solution high performance liquid chromatography, obtain amitraz standard curve;

[0043]B. Take 0.08g of the sample to be tested, use N, N-dimethylformamide solution to dissolve, dilute, constant volume, and filter, wherein a 50ml volumetric flask is used for constant volume, and a 0.45μm filter membrane is used to filter to prepare the sample The solution is detected by a high-performance liquid chromatography method to obtain a high-performance liquid chromatogram of the sample solution;

[0044] C, according to the high performance liquid chromatogram of the sample solution obt...

Embodiment 2

[0047] A detection method for amitraz, comprising the following steps:

[0048] A. Take amitraz standard substance, dissolve it with N,N-dimethylformamide, and filter at constant volume to prepare 0.07mg / ml, 0.08mg / ml, 0.09mg / ml, 0.1mg / ml, 0.2mg / ml ml, 0.3mg / ml, 0.4mg / ml series concentration standard solution, use high performance liquid chromatography to detect, obtain series concentration standard solution high performance liquid chromatography, obtain amitraz standard curve;

[0049] B. Take 0.01g of the sample to be tested, use N,N-dimethylformamide solution to dissolve, dilute, constant volume, and filter, in which a 50ml volumetric flask is used for constant volume, and a 0.45μm membrane filter is used to prepare the sample The solution is detected by a high-performance liquid chromatography method to obtain a high-performance liquid chromatogram of the sample solution;

[0050] C, according to the high performance liquid chromatogram of the sample solution obtained and...

Embodiment 3

[0053] A detection method for amitraz, comprising the following steps:

[0054] A. Take amitraz standard substance, dissolve it with N,N-dimethylformamide, and filter at constant volume to prepare 0.07mg / ml, 0.08mg / ml, 0.09mg / ml, 0.1mg / ml, 0.2mg / ml ml, 0.3mg / ml, 0.4mg / ml series concentration standard solution, use high performance liquid chromatography to detect, obtain series concentration standard solution high performance liquid chromatography, obtain amitraz standard curve;

[0055] B. Take 0.16g of the sample to be tested, use N,N-dimethylformamide solution to dissolve, dilute, constant volume, and filter, wherein a 100ml volumetric flask is used for constant volume, and a 0.45μm filter membrane is used to filter to prepare the sample The solution is detected by a high-performance liquid chromatography method to obtain a high-performance liquid chromatogram of the sample solution;

[0056] C, according to the high performance liquid chromatogram of the sample solution ob...

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Abstract

A method for detecting the content of amitraz includes the following steps that firstly, an amitraz standard substance is taken, standard solutions of a series of concentrations are prepared, detection is carried out with the high performance liquid chromatography, high performance liquid chromatograms of the standard solutions of the series of concentrations are obtained, and an amitraz standard curve is obtained; secondly, a sample to be detected is taken, a sample solution is prepared, detection is carried out with the high performance liquid chromatography, and a high performance liquid chromatogram of the sample solution is obtained; thirdly, the content of amitraz in the sample to be detected is calculated with the external standard method according to the obtained high performance liquid chromatogram of the sample solution and the obtained amitraz standard curve. According to the chromatographic conditions of the high performance liquid chromatography adopted in the first step and the second step, octadecylsilane chemically bonded silica serves as a packed column, an acetonitrile-phosphoric acid solution serves as a mobile phase, the flow velocity of the mobile phase is 0.5-1.5 ml / min, the column temperature is 20-40 DEG C, and the detection wavelength is 250-350 nm. By the adoption of the high performance liquid chromatography, the method for detecting the content of amitraz is easy, convenient and fast to implement, high in university, short in peak appearance time, capable of saving time and labor, economical and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry detection, in particular to a method for detecting the content of amitraz. Background technique [0002] Amitraz (C 19 h 23 N 3 ), is N-methyl-bis(2,4-xylbeniminomethyl)amine, and its molecular structure is: [0003] [0004] Amitraz is a broad-spectrum acaricide with moderate toxicity. It mainly has contact killing effect, and also has stomach poisoning, fumigation, antifeedant and repelling effects. It has certain penetrating and systemic properties on plants. It is effective against mite eggs, and effective against harmful mites that are resistant to some other acaricides. It has good quick-acting and long-lasting effects. It is mainly used to prevent and control mites in fruit trees, flowers, strawberries and other agricultural and horticultural crops, especially for citrus mites. It is also used to control cotton bollworm and red bollworm. Amitraz is the most effective v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06
Inventor 李成应陈静虹林孝崇徐奇清陶映娴付良凯
Owner FOSHAN NANHAI EASTERN ALONG PHARMA CO LTD
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