111 results about "Bollworm" patented technology

This invention relates to the use of a combination of different proteins insecticidal to Helicoverpa zea or Helicoverpa armigerain an insect resistance management process, wherein such proteins are: a) a Cry2A protein such as Cry2Aa, Cry2Ab, or Cry2Ae and b) a Cry1A, Cry1F or VIP3A protein, particularly wherein such proteins binds saturably to the insect midgut membrane of Helicoverpa zea or Helicoverpa armigera, as well as plants and seeds expressing such combination of proteins, which are used to delay or prevent the development of resistance in populations of such insect species.

The invention discloses a fluoro methoxylpyrazole-containing o-formylaminobenzamide compound shown as a structural formula (I) or agriculturally suitable salts thereof, a synthesis method and application thereof. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has the advantages of effectiveness, low toxicity, low cost and environmental safety and can be used for preventingand removing various pests in the fields of agriculture and horticulture or pests parasitized on animals. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has very high activityon lepidopteran pests (plutella xylostella, spodoptera exigua and cotton bollworm), hemipteran pests (black peach aphid and aphis craccivora koch), homopteran pests (nilaparvata lugens), dipteran pests (liriomyza trifolii) and leaf beetle pests (phaedon conchleariae). Particularly, good effect on the lepidopteran pests, the hemipteran pests and the dipteran pests can be achieved under the condition of low dosage of the compound.

The artificial trichogramma breeding and producing method includes the following steps: 1) producing parasitic egg via selecting and storing Chinese oak silkworm cocoon, heating cocoon, collecting moth, paunching to collect egg, washing egg, sterilizing and drying egg in the air; 2) trichogramma breeding; 3) inoculating trichogramma; 4) laying egg and eliminating adult trichogramma; 5) growing and culturing; 6) selecting product; 7) making trichogramma card; 8) maintaining trichogramma card; and trichogramma card warming to release. The present invention is one optimal trichogramma selecting and breeding technology for producing great amount of high quality trichogramma for biological agricultural pest preventing and controlling.

The invention relates to a pesticide composition with a synergy effect. The pesticide composition is characterized in that effective components comprise methoxyfenozide and emamectin benzoate, wherein the mass ratio of the methoxyfenozide and the emamectin benzoate is 100:1-1:1. The composition has obvious synergy effect and can reduce the pesticide dosage and delay the resistance development. The composition can be used for preventing and controlling lepidoptera pests and is particularly suitable for preventing and controlling noctuidae pests, such as spodoptera exigua, prodenia litura, cotton bollworm, and the like.

The invention relates to the field of genetic engineering for plant, which provides an insect killing gene, encoded protein thereof and application of the same. The invention synthesizes CmCry2Aa insect killing protein gene by optimized codon design of cotton, and further obtains Cm1Cry2Aa insect killing protein gene which loses 29 amino acids at N terminal of the encoded protein, and constructs plant expression carrier respectively. Expert evidence of genetic modified tobacco indicates that CmCry2Aa and Cm1Cry2Aa insects killing gene has evident resistance to boll worm.

The invention provides a biological assaying method for evaluating the effect of transgene cotton on chrysopa pallens. The method comprises the following steps: paving agar on the bottom of a culture dish; placing transgene cotton leaves on the surface of the agar, inoculating newly-hatched helicoverpa armigera larvae; feeding newly-hatched chrysopa pallens larvae for 1 to 3 days with pea aphid, then feeding the chrysopa pallens larvae with the helicoverpa armigera larvae which has been fed by the transgene cotton leaves for 1 to 3 days; observing the growth situation of the chrysopa pallens larvae since the first day when the chrysopa pallens larvae is fed with the helicoverpa armigera larvae until the chrysopa pallens larvae pupate and perform eclosion; setting a reference group; comparing the differences of the experiment group and the reference group on the indexes of pupation rate, eclosion rate, larva weight, imago weight, and total growth time, and analyzing the difference significance through variance analysis so as to evaluate the effect of transgene cotton on chrysopa pallens. The application range of the biological assaying method is wide. The effect of high dose protein in transgene plants on predators can be effectively measured by utilizing the nutrition relationship evaluation of transgene plant-pest-predator.

An insecticide for the lepidopterous pests (beet no turnal moth, bollworm, plutella xylostella, etc) is prepared from methylamino abamectin benzoate, hexafluomuron, assistant and solvent through proportionally mixing. Its advantages are high effect, broad spectrum and no environmental pollution.

The invention relates to a pesticide insecticidal composition, the effective components of which contain (A) spirotetramat and (B), i.e. one of amide insecticides. Specifically, A and B are in a mass percentage of 1-80:80-1, and the content of A and B in the compound preparation is 1-80%. The compound pesticide composition prepared by the invention can be prepared into missible oil, suspending agents, wettable powder, water dispersible granules, emulsions in water and other dosage forms by methods well known to technicians in the field. The insecticidal composition provided by the invention is suitable for controlling piercing-sucking mouthpart pests in vegetables and fruits, and is especially suitable for controlling aphids, whiteflies, mealybugs, scale insects, plant hoppers, thrips, Empoasca pirisuga Matumura, as well as lepidoptera, coleoptera pests like diamondback moths, cotton bollworms and the like.

A process for culturing the Bacillus subtilis coming from sea to obtain the insecticiding product for more agricultural pests includes bioactive screening to the marine microbes in Chinese sea to obtain a high-activity Bacillus subtilis strain, and culturing it in fermenting culture medium to obtain said active insecticiding product. Its advantages are high killing activity to agricultural pests, broad spectrum, and no environmental pollution.

The invention relates to the technical field of agriculture microorganism genetic engineering, in particular to separation and cloning of a Helicoverpa armigera cadherin gene fragment hacad1and encoded polypeptide fragment HaCad1. By separation, the cadherin gene fragment hacad1 of 735bp is obtained from the midintestine of the Helicoverpa armigera, an encoded product thereof is the polypeptide fragment HaCad1 which can be combined with Bt toxin CrylAc10. Biological experiments indicate the polypeptide prepared in the invention has very strong enhancing insecticidal activity on insecticidal crystal protein CrylAc10 of lepidoptera insects. The invention also includes preparation and an application for recombinant Escherichia coli EMB1104 expressing the Helicoverpa armigera cadherin fragment HaCad1. The recombinant Escherichia coli EMB1104 is preserved in CCTCC, the preservation number is CCTCC NO: M209010.

The invention discloses an insecticidal composition containing sulfoxaflor and carbamates. The insecticidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is the sulfoxaflor; the active ingredient B is any of the following insecticides: carbaryl, indoxacarb, metolcarb, fenobucarb, and isoprocarb; and the weight ratio of the active ingredient A to the active ingredient B is 1-50 percent:1-80 percent. The composition can control various insect pests, has a remarkable synergistic effect, widens a bactericidal spectrum, and has relatively high activity on cotton bollworms, aphids, rice planthoppers, rice leafhoppers, and Cnaphalocrocis medinalis as well as Plutella xylostella, cabbage caterpillars and beet armyworms on cruciferous vegetables; the using amount of pesticides is reduced, the residual amount of the pesticides on crops is reduced, and the environmental pollution is reduced; and the insecticidal composition is safe to people and livestock, has high environmental compatibility, and ensures that pests cannot produce the pesticide resistance easily.

An integrated method for ecologically preventing and eliminating ballworm features the integration of the winter irrigation for eliminating its overwintering pupae, planting corn zone as attraction zone after the cotton is sown, applying sexual attractant in large area for capturing them, use of trichogramma as the natural enemy of ballworm, and applying low-poison efficient agricultural chemical.

A method for quickly detecting insect resisting cotton of transgenosis includes budding up cottonseed of transgenosis insect vesisting cotton to be detected, mixing it with manual feed of cotton bollworm, placing mixture in breeding box and putting larva of cotton bollworm in box, determining out death rate of larva of cotton bollworm for judging whether it is insect resisting cotton of transgenosis or not, confirming that it is if said death rate is greater than 65% or confirming that it is not if said death rate is less than 40%.

This invention publicized one kind to originate from the insect fat body organization, and to the rod-shaped viral high sensitive cell line, this invention has also publicized this cell line establishment method, as well as this cell line the use which grew into the rod-shaped viral scale metaplasia. This cell line may use for to duplicate this kind of virus, uses in the rod-shaped viral pesticide the formalization production, as well as the construction rod-shaped virus the expression vector system, the expression has commercial or the scientific value protein. Therefore it has the extremely high trade and the scientific research value.

The invention discloses a pesticide for preventing cotton bollworms on vegetable leaves. The pesticide comprises the following ingredients in parts by weight: 25-30 parts of pyrethrins, 25-30 parts of veratramine, 20-30 parts of rotenone, 18-20 parts of matrine, 6-8 parts of nicotine, 18-23 parts of stemonine, 12-15 parts of toosendainin, 10-12 parts of d-phenothrin, 25-30 parts of celastrus angulatus, 20-30 parts of dimethrin, 18-26 parts of cinnamomum burmannii oil, 0.05-0.08 part of green muscardine fungus powder, 0.06-0.08 part of phage powder, 0.5-1 part of azadirachtin, 2-7 parts of Momordica charantia Linn., 8-13 parts of camphor, 20-26 parts of herba houttuyniae and 100-150 parts of water.

The present invention discloses a new antibiotic streptomyces daliensis sp.nov. CCTCC No.M201031 and its preparation method. Its preparation method includes the following steps: drying and pretreating soil sample, using sterile water to dilute concentration, coating on separation culture medium, inverted culture in calorsat, selecting single colony, culture on culture medium, shake-flask fermentation of separated pure strain, using fermented browh water phase and fast phase sample to make biological screening, mixing the water sample, artificial fued and target insect ovum and making culture.Said invented has high activity for killing insects, can be used for killing bollworm, asparagus fern caterpillar, cabbageworm and pine moth, etc.

The invention relates to the field of biological control, in particular to an evaluation method for cotton bollworm resistance of Bt (bacillus thuringiensis)-transgenic cotton. The method comprises the following steps: leaves are collected in emergence periods of a second generation, a third generation and a fourth generation of cotton bollworms; and non-transgenic cotton is adopted as a control material; the leaves are placed in a box; hatched larval cotton bollworms are inoculated; the dead quantity and instar of the larval cotton bollworms are investigated after the hatched larval cotton bollworms are inoculated; statistical analysis is conducted; and the instar inhibition rate of the cotton bollworms is calculated. According to the method, the sample quantity is relatively less; only one cotton bollworm can be inoculated, so that labor is saved and cotton bollworm tests are reduced, that is to say, the labor and the time are saved. According to the method, evaluations are conducted separately on the emergence periods of the second generation, the third generation and the fourth generation of the cotton bollworms, so that impacts and effects of foreign protein on the cotton bollworm resistance in the cotton growth and development processes can be reflected accurately, and the life of the cotton capable of resisting the cotton bollworms is prolonged. With the adoption of the method, the use quantity of the cotton bollworms is reduced greatly; and the method is easy to operate, simple, accurate, objective and reliable.

A fast doner plasmid with singly embedded polyhedronvirus gene of bollworm and P10 gene promoter sequence is configured through inserting exogenous gene in polycloning site under control of P10, transfecting it to bollworm cell by inserting the exogenous gene and polyhydrous gene in the shuttle expression carrier, collecting recombinant virus particles, transfecting again, and detecting the exogenous gene expression level. Its advantages is short period (7-14 days).

The present invention is the breeding process of transgenic bollworm resisting cotton hybrid, Bt+CpTI. To transgenic bollworm resisting cotton SGK321, bollworm resistance identification and breeding is performed to obtain transgenic bollworm resisting cotton SGK321' with bollworm resistance isozygoty of 100 % and yield 9.5% higher than the original variety. Bollworm resisting cotton hybrid Nankang-7 is further obtained by using as SGK321'as male parent and Sumian-12 as female parent and it has high yield and high bollworm resistance and can inhibit the resistance of bollworm to the cotton.

A single embedded polyhedronvirus shuttle expression carrier of bollworm is configured through inserting the low-copy bacterium F replicon, kanamycine resistant gene and beta galactosidase gene to the polyhedrom gene site of the virus genom. The doner plasmid is used to transfer the exogenous gene to the insetion site of transposon in the said shuttle expression carrier. After bollworm cell is transfected by said DNA, the infections recombinant virus particles are formed to express exogenous gene under control of polyhedron promoter and P10 promoter.

The invention discloses a spirotetramat containing pesticide composition, which is a pesticide composition containing an active component A and an active component B, wherein the active component A is spirotetramat, the active component B is any one of the following pesticides: chlorpyrifos, MTMC (meta-tolyl-N-meth ylcarbamate) and isoprocarb, and the weight ratio of the two active components is 60:1-1:80. The composition disclosed by the invention can control various insect pests, has an obvious synergistic effect, expands the insecticidal spectrum, and has high activities to aphids, thrips, phylloxera, mealybug, aleyrodid, scale insects, rice planthopper, rice leaf folders, prodenia litura, bradysia odoriphaga, tryporyza incertulas, leafhopper, phyllocnistis citrella stainton, cotton aphid, cotton bollworms, phyllocoptes oleivorus ash, tea lesser leafhopper, plutella xylostella, cabbage caterpillar, and asparagus caterpillar; the application amount of pesticides is reduced, the residues of pesticides on crops are reduced, and the environment pollution is reduced; and the composition is safe to people and livestock and good in environmental compatibility, and pests are unlikely to produce pesticide resistance.

The invention provides a plant insecticide for preventing and treating cotton bollworm and a preparation method thereof. The mass content of each raw material component of the insecticide is: 12-19 parts of imidacloprid, 1.45-2.6 parts of ginger extract, Prochloraz 2‑4 parts, tetrahydrojatrorrhizine 0.8‑1.3 parts, ammonium molybdate 1.5‑2.7 parts, emulsifier 5‑9 parts, organic solvent 10‑16 parts, cosolvent 3‑5 parts, tea tree oil 15 parts ‑27 servings. The present invention also provides the application of the above-mentioned insecticide in cotton planting in saline-alkali land. Beneficial effects of the present invention: the insecticide prepared by the present invention has a good control effect on pests in cotton planting fields, and has good control effect on common pests in cotton planting fields: cotton bollworm, cotton bug, thrips, cotton spider mite, whitefly The control effect of the invention is significantly improved; the insecticide prepared by the invention has the same effect on the disease control of cotton plants while eliminating the pests in the cotton planting ground.

A cathepsin B gene of bollworm and its clone and recombinant expression process are disclosed. Its preparing steps include extracting overall RNA from bollworm, reverse transcription to synthesize cDNA, designing primer, polymerase chain reaction, multiplication purifying, cloning it to pGEM-T Easy vector, transferring DH5 alpha cells for sequence test, fast multiplication to obtain end-to-end CDNA (1310 bp), and expressing proteinase in prokaryotic and eukaryotic organisms to generate cathepsin B with hydrolysis activity.

This invention relates to the use of a combination of different proteins insecticidal to Helicoverpa zeaor Helicoverpa armigerain an insect resistance management process, wherein such proteins are: a) a Cry2A protein such as Cry2Aa, Cry2Ab, or Cry2Ae and b) a Cry1A, Cry1F or VIP3A protein, particularly wherein such proteins binds saturably to the insect midgut membrane of Helicoverpa zeaor Helicoverpa armigera, as well as plants and seeds expressing such combination of proteins, which are used to delay or prevent the development of resistance in populations of such insect species.