178 results about "Nanocarriers" patented technology

The invention discloses an RGD-click chemical crosslinking siRNA nano-carrier, a preparation method thereof and application of the nano-carrier in preparation of a medicine for treating secondary thyroidism, and belongs to the technical field of medicines.The nano-carrier is RGD-PEG-PLys (N), and the preparation method of the nano-carrier comprises the steps of synthesis of PLys (ss-DBCO), synthesis of RGD-PEG-PLys (N) and the like. According to the application, the nano-carrier is used for preparing siRNA composite nanoparticles; vascular endothelial targeting (RGD), dynamic stability (click crosslinking) and microenvironment responsiveness (disulfide bond) are organically combined for the first time to achieve mutual synergistic interaction, and the effect ceiling of an existing material is broken through; according to the siRNA composite nanoparticle, the silence effect of the PTH gene as long as 70 days can be achieved through single intravenous injection, the PTH inhibition rate is larger than 85%, the siRNA accumulation amount in parathyroid gland tissue is remarkably increased through RGD targeting (8.6 times that of a non-targeting group), the liver and kidney distribution amount is reduced by 60%, and the system toxicity is controllable.

A cage-like nanocarrier for targeted delivery of siRNA, and a preparation method therefor and the use thereof. The preparation method comprises: (A) mutating a negatively charged or uncharged amino acid on the inner surface of a ferritin into a positively charged amino acid; and any one or more of the following steps: (B) coupling the N-terminus of the ferritin to a functional peptide having nucleic acid affinity; (C) coupling the N-terminus of the ferritin to a functional peptide promoting lysosomal escape; (D) truncating the E-helix at the C-terminus of the ferritin; (E) coupling the C-terminus of the ferritin to a functional peptide having nucleic acid affinity; and (F) coupling the C-terminus of the ferritin to a functional peptide promoting lysosomal escape. A new nucleic-acid-loaded protein nanocage carrier is constructed by means of modifying a negatively charged inner cavity of ferritin to make same positively charged. By means of electrostatic adsorption, a negatively charged siRNA can be efficiently loaded into a ferritin nanocage, thereby significantly improving the in-vivo and in-vitro delivery stability of siRNA, lysosomal escape functions, and efficacy of targeted therapy.

The application provides a multifunctional nano particle for targeting abdominal aortic aneurysm and a preparation method and application thereof, and belongs to the field of nanobiomedicine, wherein polyphenol oxidation self-polymer nanoparticles are used as carriers, doxycycline is loaded, and a targeting ligand cRGD is modified on the surface of the nano carrier; the neovasculature in the media and adventitia of AAA sites helps accumulation of the nanoparticles in the abdominal aortic aneurysm, and the integrin αν3 beta receptor highly expressed on the surface of the diseased cell membrane can recognize the cRGD with high affinity, and then mediate the targeted endocytosis of the nanoparticles; after intravenous injection, the nanoparticles can be effectively enriched in the lesion site and achieve long-term retention, and can release DC in response to the high ROS level in the tumor microenvironment; the drug is rapidly released to take effect, and the non-specific toxic side effects are reduced; the free radical scavenging capacity of the nanoparticles can be synergized with the DC activity to treat AAA through multiple mechanisms such as anti-inflammatory, antioxidant, macrophage repolarization promotion, anti-apoptosis and calcification inhibition, and MMPs inhibition.

A self-assembling composition includes molecules of a first polymer, molecules of a positively charged second polymer, and a nucleic add. First pendant groups conjugated to pendant amine group of the first polymer include a hydrophilic polymer. Second pendant groups conjugated to the pendant amine groups include a hydrophobic group. Third pendant groups conjugated to the pendant amine groups include a group selected from a nucleoside and a nucleoside analog. Fourth pendant groups conjugated to the second polymer via pendant amine groups thereof include the hydrophilic polymer. Fifth pendant groups are conjugated to the amine groups of the second polymer include the hydrophobic group. The second polymer also includes repeat units wherein the pendant amine groups are positively charged in vivo.

This invention relates to the fields of pesticide formulations and pesticide pest control, specifically to a composite nano-slow-release suspension of emamectin benzoate and chlorantraniliprole, its preparation, and application. The nano-slow-release suspension comprises emamectin benzoate, chlorantraniliprole, a nanocarrier, a wetting agent, a dispersant, a synergist, a thickener, an antifoaming agent, an antifreeze, a preservative, and water. The nanocarrier is an NX-CMCS & MOF composite nanocarrier. Spraying this composite nano-pesticide onto crops improves the adhesion and penetration of the pesticide on leaves, enhances its resistance to rain washout, and effectively kills major crop pests such as bollworms, diamondback moths, cowpea thrips, and rice planthoppers. Compared with traditional pesticide formulations, it has a significant effect of reducing dosage and increasing efficacy, and has broad application prospects in the field of agricultural pest control and promoting green agriculture.

A kind of biotin or avidin labeled fat body and its preparation method, nano-carrier system. Biotin or avidin labeled fat body is prepared using specific amount of Bio-phospholipid or avidin-phospholipid, and the prepared fat body has the advantages of high purity, small particle size, good uniformity and high stability. Based on the biotin-avidin system, a nano-carrier system is constructed, which includes the biotin labeled fat body and the avidin modified target component. The system enables the fat body to carry any interested substance, such as viral recombinant protein, antibody and other protein drugs, nucleic acid drugs or small molecule drugs, etc., so as to be used for the treatment of diseases such as vaccines, cancer, infectious diseases and metabolic diseases.

The invention discloses a tellurium-based nano material capable of efficiently inducing copper death as well as a preparation method and application of the tellurium-based nano material. The preparation method comprises the following steps: by taking water as a medium, mixing soluble copper salt and a sulfydryl-containing compound, and stirring to react, so as to obtain a Cu-S2 solution; dropwise adding the Cu-S2 solution into the TeO2 nano-carrier solution, and carrying out stirring reaction; and after the reaction is finished, carrying out solid-liquid separation, taking the solid, and resuspending with methanol to obtain the TeO2 coated Cu-S2 nano material. The preparation method is simple and rapid, large-scale production can be achieved, and the obtained TeO2 coated Cu-S2 nano material can efficiently induce cancer cells to generate copper death, is effective to various cancer cells and has potential clinical transformation application prospects.

The invention relates to a hybrid nanocarrier system comprising engineered metal nanoparticles (MeNP), a flavonoid (Fl), a Raman-active molecule (RAM), poly(allylamine hydrochloride) (PAH), genetic material (GM), and poly(styrene sulfonate) (PSS), designed for use in fields such as gene therapy, cancer treatment, chemotherapy, treatment of genetic diseases, innovative vaccine technologies, the production of innovative antibiotics for antibacterial and antimicrobial therapies, drug delivery, the dietary supplement market, Raman imaging systems, biotechnological applications, and the agrochemical industry.

The invention relates to the technical field of drug carriers, in particular to a mesoporous nanomaterial coupled with a CCR2 antibody and a preparation method and application of the mesoporous nanomaterial. According to the invention, the aminated mesoporous material is used for loading the drug and is coupled with the CCR2 antibody, so that the obtained CCR2 antibody coupled mesoporous nano material can target CCR2 + macrophages, the bioavailability of drugs such as matrine is improved, and the side effect is reduced. According to the invention, leading-edge technical elements such as a mesoporous nano-carrier, CCR2 antibody targeted modification and macrophage precise delivery are introduced, so that a carrier-drug-antibody ternary innovative combination system is formed.

The application relates to a preparation method of natural cyclic lipopeptide nano-encapsulates with high oil content, and the steps are as follows: natural emulsifiers, glycerol, oil and water are weighed, the natural emulsifiers comprise sodium subtilipetrol and polyglycerol-10 oleate; the natural emulsifiers and the glycerol are mixed and heated and stirred, the oil is added after being dissolved, and a gel phase is obtained; water is added to the gel phase until the gel phase is completely dispersed, and natural cyclic lipopeptide nano-encapsulates are obtained. The compounding and synergistic effect of the sodium subtilipetrol and the polyglycerol-10 oleate is used in the application, compared with two single emulsifiers, the oil loading content is improved and the particle size is reduced. The natural nano-emulsion with high oil content prepared by the application has the advantages of low emulsifier dosage, simple process, small particle size (less than 600 nm), high oil content (more than 20%) of the prepared nano-emulsion, strong stability, high active substance loading content, good penetration effect and the like, is suitable for most liquid oils, and has high application value in the field of cosmetics.

The invention belongs to the technical field of biological medicines, and particularly provides an ALO-coated F127-MOF nano-composite as well as a preparation method and application thereof. According to the ALO (at) F127-MOF nano compound, UiO-66-NH2 MOF with a pluronic F-127 coating is used as a nano carrier, and aloperine is loaded on the nano carrier. The nano compound is used for treating ALI, the solubility, stability and bioavailability of the traditional Chinese medicine aloperine are effectively improved, and the synergistic effect of sustained release of aloperine, ROS neutralization and effective targeting is achieved. The aerosolization-based delivery approach ensures better oxygenation, lower systemic toxicity, and better pulmonary deposition. The invention provides a new treatment method for noninvasive acute lung injury treatment.

The invention belongs to the technical field of biomedical materials and targeted drug delivery systems, and discloses a targeted controlled-release drug delivery system based on a polymer nano-carrier and a preparation method thereof.The system is of a three-layer structure and comprises a core layer formed by taking polyvinyl alcohol as a matrix and used for efficiently entrapping a drug, and an outer layer formed by taking polyvinyl alcohol as a matrix and used for efficiently entrapping the drug; the shell layer formed by the polylactic acid-glycolic acid copolymer is used for providing structural stability and slow release capability, and the shell layer formed by the polyvinyl alcohol-hydroformylated glucan grafted copolymer is used for realizing microenvironment response release. And the surface of the shell layer is covalently modified with a tumor membrane targeting aptamer and a microenvironment response peptide, so that accurate recognition and local trigger release can be realized. The system has good particle size stability, responsive release ability and tumor cell targeted enrichment effect, and is suitable for efficient delivery and controlled release of various antitumor drugs.

The invention provides a dual-targeting nano-material photosensitizer for cervical cancer as well as a preparation method and application of the dual-targeting nano-material photosensitizer. The folic acid modified poly-beta-cyclodextrin (poly-beta-CD) is used as a nano-carrier and is used for loading a photosensitizer PAP (PAP is pyropheophorbide a covalently connected PAAKRVKLD) coupled with a nuclear localization signal (NLSs). The finally prepared FA-CD-coated PAP nano material can specifically recognize cervical cancer cells of overexpressed folate receptors (FR), so that accumulation in tumor cells is remarkably enhanced. In addition, the encapsulated PAP can achieve accurate cell nucleus localization. Under an illumination condition, reactive oxygen species (ROS) generated by PAP accumulated in nuclei can instantly oxidize and damage a DNA chain or DNA repair enzyme, so that cell death is directly induced, and the anti-tumor effect of targeted photodynamic is greatly enhanced. The novel dual-targeting nano drug-loading system prepared by the invention solves the problems of poor targeting and biological solubility and limited treatment effect of a traditional photosensitizer, and provides an innovative intelligent targeting delivery strategy for in-situ cell nucleus photodynamic therapy.

Described herein is a bias-free high-throughput and high-yield continuous isoelectric fractionation (CIF) nanocarrier fractionation technique based on distinct isoelectric points. The nanocarrier fractionation platform is enabled by a robust and tunable linear pH profile provided by water-splitting at a bipolar membrane and stabilized by flow without ampholytes.

The application of poly(D-lactic acid) and poly(D-lactic acid-glycolic acid) copolymers in constructing micro / nanocarriers for antitumor drugs belongs to the field of biomedical polymer materials and nanomedicine. The antitumor drugs are small-molecule chemotherapeutic agents, small-molecule photosensitizers, small-molecule immunotherapeutic agents, and small-molecule radiosensitizers. The particle size of the poly(D-lactic acid) and poly(D-lactic acid-glycolic acid) copolymers is 10–5000 nm. The effective concentration of the poly(D-lactic acid) and poly(D-lactic acid-glycolic acid) copolymers is 1–500 mg / kg. The antitumor mechanism of the poly(D-lactic acid) and poly(D-lactic acid-glycolic acid) copolymers includes increasing the activity of effector T cells in the tumor microenvironment and increasing the concentration of cytokines such as IFN-γ at the tumor site. In the field of tumor therapy, PLA and PLGA materials composed of D-lactic acid have shown good effects in inhibiting tumor growth, prolonging the survival period of tumor-bearing mice, and activating antitumor immune responses.

An adjuvant polypeptide, a nano-carrier and application of the nano-carrier in vaccine adjuvant, the present application relates to the field of vaccine.The present application solves the problems of poor antigen compatibility, lack of targeting, low delivery efficiency and insufficient preparation stability of traditional adjuvant.The amino acid sequence of the adjuvant polypeptide Adp is VLGKLAKVAI.The nano-carrier is prepared from the adjuvant polypeptide and antigen.The nano-carrier is prepared by mixing the adjuvant polypeptide and antigen through in-situ radical polymerization.The application of the nano-carrier in vaccine adjuvant.The present application screens a new vaccine adjuvant by bioinformatics method, so that it has the abilities of dendritic cell targeting and intracellular STING pathway activation, thereby enhancing the cross-presentation of antigen.Based on this, the new adjuvant is coupled with monomer, and an intelligent vaccine delivery system is constructed by in-situ polymerization method, so as to realize the efficient delivery of tumor cell membrane protein antigen and improve the immunotherapy effect of tumor vaccine.

Present invention relates to an apparatus (0) for producing nanocarriers and / or nanoformulation and a process for producing a nanocarrier and / or a nanoformulation by means of this apparatus (0). According to inventive preparation, a first liquid phase (A) and the second liquid phase (B) are mixed first to give the primary mixture (A+B) by means of a static mixer (4). In a subsequence mixing step primary mixture (A+B) is diluted with a third liquid (C). An important aspect of apparatus (0) is the arrangement of the static mixer (4) inside a linear pipe (7) conducting third liquid phase (C). Thus, the primary mixture (A+B) exiting the mixer (4) is instantaneously diluted with (C) to give secondary mixture (A+B+C). The volume flow of the third mixture (C) is chosen larger than the volume flow of the primary mixture (A+B). By these measures, nanocarriers with improved morphology and homogeneity are produced. Encapsulation efficiency was enhanced as well.

This invention belongs to the field of nanocarrier and immunotherapy technology, specifically relating to a lipid nanoparticle targeting tumor-associated macrophages, a combined delivery method, and its application in tumor immunotherapy. The lipid nanoparticles targeting CD206 tumor-associated macrophages constructed in this invention are used for efficient in vivo delivery of multifunctional nucleic acid molecules, achieving immune regulation, gene editing, and anti-tumor therapy. This invention achieves targeted delivery and multifunctional synergistic effects through lipid particle design, nucleic acid sequence design, and in vitro and in vivo functional verification. Through in vivo delivery of ipLNPs, macrophages can simultaneously achieve: 1) expression of anti-PD-L1 antibodies, blocking immunosuppressive signals; 2) expression of HER2-CAR structures, enhancing phagocytosis and killing ability against tumors; and 3) remodeling of the tumor immune microenvironment through CRISPR / Cas9-mediated FN1 knockout. The lipid nanoparticles constructed using this invention can solve the problem of insufficient targeting of immune cells in the tumor microenvironment by traditional LNP delivery systems.

The invention discloses a drug-loaded nanoparticle as well as a preparation method and application thereof. The drug-loaded nanoparticles comprise active components and a carrier, wherein the active components comprise a hydrophobic small-molecule inhibitor and PAEMA; the carrier comprises an amphiphilic polymer bonded with a photosensitizer; the amphiphilic polymer bonded with the photosensitizer is coated with a hydrophobic small-molecule inhibitor and PAEMA (Polyacrylamide); wherein the amphiphilic polymer bonded with the photosensitizer comprises the photosensitizer, a hydrophilic chain segment and a hydrophobic chain segment. Precise drug delivery is achieved through characteristic transformation of the nano-carrier driven by tumor acidity, M2 type macrophages can be removed, dendritic cells can be induced to be mature, tumor cells can be killed, and therefore the synergistic anti-tumor effect of photodynamic therapy and immunotherapy is enhanced. Therefore, various drugs can be accurately delivered to target cells with different spatial distributions, so that the cell killing level and the antigen presenting cell mediated immunocompetence are enhanced at the same time.

This invention relates to a glucose-responsive antibacterial nanocomposite microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. The glucose-responsive antibacterial nanocomposite microneedle patch is an integral soluble microneedle. The functional component of the microneedle material is a nanocomposite CIP / GOx@ZIF-8, which uses ZIF-8 as a nanocarrier, co-loading ciprofloxacin hydrochloride and glucose oxidase. This invention synthesizes CIP / GOx@ZIF-8 using a one-step self-assembly method, achieving co-loading of CIP HCl and GOx. Furthermore, this invention further prepares CIP / GOx@ZIF-8 into microneedles. The preparation method is simple, easy to use, and allows for self-administration. Topical application to skin wounds is safer and more effective than systemic administration, further improving drug delivery efficiency and delivering therapeutic agents deep into the wound, promoting wound healing. This invention combines multiple therapeutic mechanisms, significantly enhancing the antibacterial properties of the microneedles and achieving the treatment of bacterially infected diabetic wounds.

This invention provides methods for predicting functional and / or structural properties of a nanocarrier that is a non-viral delivery system, along with related methods, systems and products. Functional properties of a nanocarrier may include transfection efficiency, structural properties of a nanocarrier characterise physico-chemical properties such as polydispersity, size and zeta potential. Methods include computational modelling such as machine learning and related statistical techniques.

The invention belongs to the technical field of genetic engineering, and particularly relates to a bovine group A rotavirus multi-epitope fusion protein and application thereof. The invention provides a bovine group A rotavirus multi-epitope peptide based on a ferritin nano-carrier, a fusion protein and application of the bovine group A rotavirus multi-epitope peptide and the fusion protein. The multi-epitope peptide disclosed by the invention is a multi-epitope fusion antigen which is formed by splicing cytotoxic T lymphocyte epitopes, helper T cell epitopes and B cell epitopes with high conservative property and strong immunogenicity and is formed by screening and obtaining the cytotoxic T lymphocyte epitopes, helper T cell epitopes and B cell epitopes with high conservative property and strong immunogenicity on the basis of VP4 and VP7 protein sequences of bovine group A rotaviruses by utilizing an immunoinformatics technology. Immunological evaluation shows that the monoclonal antibody has good antigen specificity and neutralizing activity. Animal experiment results show that the epitope peptide and the fusion protein can induce an organism to generate high-level neutralizing antibodies aiming at BRVA G6, G8, G10 and other multi-genotype strains, and the broad spectrum and durability of immune protection are remarkably improved.

The application relates to the technical field of medical nanobiomaterials, and provides a preparation method of a GOX-CAT-HAase-Hb natural enzyme nanogel for pancreatic cancer starvation treatment, which solves the key problems of complex nanocarrier process, low enzyme encapsulation rate, insufficient drug loading, poor dispersibility, easy degradation and inactivation of free enzymes, low synergistic efficiency and poor biocompatibility in the pancreatic cancer starvation treatment; the method comprises the following steps: S1, polyallylamine is dissolved in a HEPES buffer solution, PEG2000-NHS is reacted at room temperature, glycidyl methacrylate is reacted at 60 DEG C, and a positive polymer solution is obtained through dialysis; S2, GOX, HAase, CAT and Hb are respectively modified by NAS acrylation, and are freeze-dried after dialysis and purification; S3, the four are mixed in a molar ratio of 1:1:1:1, positive polymer and acrylamide monomers are added, an initiator is added after nitrogen deoxygenation, polymerization is carried out, and a natural enzyme nanogel is obtained through dialysis and purification.

The application discloses a plant source biological regulator delivery method based on nano carriers, and particularly relates to the technical field of nano materials, and comprises the following steps: constructing a locking risk map based on soil mineral distribution, modifying the structure of the nano carriers in a high adsorption area in response, and regulating the release rhythm; combining the release history with the characteristics of the soil microenvironment to implement multi-level regulation on the structure of the nano carriers, and dynamically adjusting the release path; collecting rhizosphere distribution and plant response data through nano probes during application, modeling and optimizing the next round of delivery strategy, and realizing closed-loop accurate delivery of the plant source biological regulator; through the construction of the soil mineral map, the high adsorption area is identified before application, the release rhythm is controlled by using the responsive nano carrier structure, the release path and dose are dynamically adjusted by combining the data collected by the rhizosphere probe, multi-cycle, regional and closed-loop regulator delivery is realized, and the application accuracy and environmental adaptability are improved.

Micelle compositions comprising a hedgehog pathway inhibitor and their use in treating skin diseases, conditions, or disorders, such as skin cancers, including basal cell carcinoma, are disclosed.

This invention discloses a mesoporous silica nanocarrier for enhancing oral intestinal retention, its preparation method, and its application, belonging to the field of nanomedicine delivery technology. The preparation method of the mesoporous silica nanocarrier includes the following steps: dissolving a template agent and an alkaline catalyst in an ethanol-water solution, adding a silica source material, reacting at 100±50 rpm, and then calcining at high temperature to obtain the mesoporous silica nanocarrier. This invention successfully prepared helical mesoporous silica nanoparticles by controlling the stirring rate during the nanoparticle synthesis process. Due to their unique geometric structure, these nanoparticles exhibit optimal in vitro mucus permeability and intestinal retention characteristics, significantly enhancing the therapeutic effect of loaded drugs and improving drug bioavailability in the intestine. This invention provides a new strategy for constructing efficient oral targeted drug delivery systems and achieving precision treatment of IBD.

The invention discloses a pH and enzyme dual-response type targeted DNA nano-carrier for relieving tumor cell hypoxia as well as a preparation method and application of the pH and enzyme dual-response type targeted DNA nano-carrier. The DNA nano-carrier comprises m tandem repeat units, each tandem repeat unit comprises a long single-stranded DNA repeat unit, three complementary short single-stranded DNAs and siRNA, each of the three complementary short single-stranded DNA sequences comprises a functional region and a base complementary region, and the functional region is a pH-responsive nucleic acid complementary sequence or a nucleic acid aptamer sequence for specifically targeting cancer cells; siRNA is a gene therapeutic agent for relieving tumor hypoxia, an extended positive-sense strand of siRNA comprises a 5 '-terminal extended pH response sequence and a positive-sense strand, and the 5'-terminal extended pH response sequence is complementary with a pH-responsive nucleic acid complementary sequence of the complementary short single-stranded DNA. The nano-drug carrier has excellent drug loading capacity and targeting property, can deliver anti-cancer drugs such as siRNA in a targeted manner, responds to a slightly acidic / enzyme environment of tumor cells and is quickly released, and the anti-tumor treatment effect is improved.

The application discloses a kind of dual targeting / drug-loaded reduction-sensitive nano-carrier, and the micelle is formed by the micelle of block polymer by solvent evaporation method, and the multi-block polymer is Tel-PEG-ss-PCL, the hydrophilic end of which is PEG with targeting group Tel, and the hydrophobic end is PCL, which is a core-shell structure, the core is the hydrophobic end PCL, and the shell is the hydrophilic block with targeting group Tel.The nano-carrier selects the polymer micelle with hydrophilic end polyethylene glycol and hydrophobic end polycaprolactone as drug carrier to improve the problem of poor water solubility of drug;Tel, an angiotensin II type receptor (AT1R) ligand, is used to simultaneously target CAFs and tumor cells overexpressing AT1R, kill tumor cells and CAFs, and double drug loading to overcome drug resistance;Meanwhile, disulfide bond is used to respond to high concentration glutathione (GSH) in tumor cells to achieve precise control release of drug in cells.The nano-carrier realizes the treatment effect of solubilization of poorly soluble drug, tumor and CAFs dual targeting and reversal of drug resistance.

The invention relates to the technical field of biological materials, in particular to an amphiphilic polyamino acid nano-vesicle and a preparation method and application thereof, and polyamino acid has a structure as shown in a formula I. The nano-vesicle is obtained by self-assembling polyamino acid with a specific block ratio in an organic solvent / water or water-containing solvent system. According to the invention, the nano-carrier with a nano-vesicle structure with a uniform particle size is prepared by adopting a nano-precipitation method. The nano-carrier has good chemical stability, biocompatibility, high loading capacity and uniform size and morphology, so that the problem that a traditional polyamino acid material is non-uniform and uncontrollable in morphology is solved, and the nano-carrier can be applied to the fields of drug delivery, tumor treatment and the like. Formula I

The application discloses a kind of nanocarrier of cosmetic active substance, micro-nano flow control preparation method and application, it is related to nanocarrier technology and cosmetic field.The application includes the following steps: S1, preparation of lipid phase, phospholipid and sterol are uniformly dissolved in ethanol, obtain lipid phase;S2, preparation of water phase, cyanate fruit polysaccharide and polypeptide are uniformly dissolved in distilled water, after uniform dissolution, centrifugation, take supernatant, obtain water phase;S3, preparation of nanocarrier, lipid phase and water phase are transported to the inside of micro-nano flow control chip and combined, lipid phase and water phase are combined to form nanocarrier in the inside of micro-nano flow control chip, obtain precursor solution containing nanocarrier;S4, screening nanocarrier, precursor solution is reduced pressure distillation, then centrifugal removal organic solvent and free un-wrapped component, obtain nanocarrier.Polypeptide is dissolved in water phase, can keep the active conformation of polypeptide peptide chain in larger amount, so that nanocarrier can deliver active substance to the specified structure layer of skin.