316 results about "Nanocarriers" patented technology

The invention discloses a preparation method and application of plant-derived extruded nano-vesicles, and relates to the technical field of biological medicines. The preparation method of the plant-derived extruded nano-vesicles comprises the following steps: juicing cleaned plant roots, stems, leaves, flowers or fruits to obtain plant juice, and filtering with gauze to obtain first filtrate; centrifuging the first filtrate at a low speed of 300-6000 * g to obtain a second filtrate; performing 8,000-20,000 * g centrifugation on the second filtrate for 0.5-3 hours at the temperature of 0-5 DEG C, so as to obtain a first precipitate; adding the first precipitate into a buffer solution, carrying out ultrasonic treatment, diluting, and sequentially passing through a filter membrane with the pore diameter of 0.4-10 microns and a filter membrane with the pore diameter of 0.1-0.4 microns, so as to obtain a third filtrate; and centrifuging the third filtrate, taking the precipitate, and resuspending to obtain the extruded nano-vesicles. Experimental detection shows that the extruded vesicles are similar to natural vesicles in characteristics, but the yield is far higher than that of the natural vesicles, and the extruded vesicles can serve as nano-carriers to be applied to a foundation or clinic.

The invention relates to a multi-effect bionic liposome transdermal repair carrier and a preparation method and application thereof.The multi-effect bionic liposome transdermal repair carrier comprises active ingredients and a nano-carrier, and the active ingredients comprise sialic acid, saccharomycetes / rice fermentation product filtrate, arginine / lysine polypeptide and vitamin E in the mass ratio of (1-10): (2-8): (0.1-1): (0.5-4); the nano-carrier is prepared from the following raw materials: phospholipid, an emulsifier, polyhydric alcohol and water. Compared with the prior art, the anti-aging, whitening and moisturizing composition has the advantages of enhancing skin moisturizing and anti-oxidation effects, promoting collagen synthesis and inhibiting tyrosinase to achieve remarkable anti-aging, whitening and moisturizing effects and the like.

The invention discloses an RGD-click chemical crosslinking siRNA nano-carrier, a preparation method thereof and application of the nano-carrier in preparation of a medicine for treating secondary thyroidism, and belongs to the technical field of medicines.The nano-carrier is RGD-PEG-PLys (N), and the preparation method of the nano-carrier comprises the steps of synthesis of PLys (ss-DBCO), synthesis of RGD-PEG-PLys (N) and the like. According to the application, the nano-carrier is used for preparing siRNA composite nanoparticles; vascular endothelial targeting (RGD), dynamic stability (click crosslinking) and microenvironment responsiveness (disulfide bond) are organically combined for the first time to achieve mutual synergistic interaction, and the effect ceiling of an existing material is broken through; according to the siRNA composite nanoparticle, the silence effect of the PTH gene as long as 70 days can be achieved through single intravenous injection, the PTH inhibition rate is larger than 85%, the siRNA accumulation amount in parathyroid gland tissue is remarkably increased through RGD targeting (8.6 times that of a non-targeting group), the liver and kidney distribution amount is reduced by 60%, and the system toxicity is controllable.

The invention belongs to the technical field of preparation of biological materials, and particularly discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application of the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The preparation method comprises the following steps: dissolving freeze-dried sea cucumber flower powder in deionized water, adjusting the pH value to be alkaline, heating and stirring, centrifuging, discarding the precipitate, adjusting the pH value to be acidic, centrifuging, collecting the precipitate, and freeze-drying to obtain sea cucumber flower protein powder; preparing a reaction solution, adding simulated gastric juice and simulated intestinal juice, stirring for reaction, inactivating protease, centrifuging, taking supernatant, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; the preparation method comprises the following steps: preparing a solution from sea cucumber, adding FeCl2. 4H2O and ascorbic acid, adjusting the pH value to be acidic, carrying out heating reaction, centrifuging, discarding the supernatant, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The invention discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application thereof. The carrier can promote absorption of an organism to iron, a relatively high iron ion release rate is maintained in an intestinal environment, and the in-vivo bioavailability of iron ions is improved.

The invention relates to the technical field of agricultural biology, and particularly provides a dsRNA nanocrystallization preparation for preventing and treating areca yellows as well as a preparation method and application of the dsRNA nanocrystallization preparation. Areca yellows are a destructive disease caused by areca symptomless virus type 1 (APV1), eight key target gene segments of an APV1 virus genome are screened, specific double-stranded RNA is designed, a nano-carrier is used for wrapping and delivering a dsRNA mixture obtained by transcription of the eight target gene segments, and the areca yellows are obtained by screening. And a nanocrystallization preparation for improving the environmental stability and RNA interference efficiency of the dsRNA is prepared. Experimental results show that the nanocrystallization preparation can effectively inhibit the key gene expression of APV1 virus, reduce the virus load and improve the resistance of areca catechu to yellows. The invention provides an accurate, efficient and environment-friendly biological prevention and control strategy, and has a wide agricultural application prospect.

The invention discloses a sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity and a preparation method and application thereof, belongs to the technical field of functional protein peptides, and particularly relates to a preparation method of the sea cucumber flower self-assembled polypeptide-selenium nano-carrier with antioxidant activity, which comprises the following steps: heating a sea cucumber flower protein solution; the preparation method comprises the following steps: mixing sea cucumber with simulated gastric juice, reacting for 30-150 minutes, then adding simulated intestinal juice, reacting for 20-120 minutes, carrying out enzyme deactivation treatment, centrifuging, taking supernate, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; and mixing the sea cucumber flower self-assembled polypeptide with deionized water to prepare a sea cucumber flower self-assembled polypeptide solution, mixing the sea cucumber flower self-assembled polypeptide solution with a sodium selenite solution and a phosphate buffer solution, reacting for 20-60 minutes, washing with an ethanol solution, centrifugally collecting precipitate, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-selenium nano-carrier. The sea cucumber flower self-assembled polypeptide-selenium nano-carrier prepared by the invention has good antioxidant activity and potential of effectively delivering selenium to an organism.

The invention provides a bolete concentrated powder composition lipid carrier with an anti-aging effect as well as a preparation method and application of the bolete concentrated powder composition lipid carrier. The lipid carrier is prepared from the following substances in percentage by mass: 10 to 40 percent of bolete extract, 0.2 to 0.8 percent of antioxidant, 45 to 66 percent of solid lipid, 10 to 16 percent of liquid lipid and 4.2 to 7.8 percent of surfactant. According to the bolete concentrated powder composition lipid carrier provided by the invention, liquid lipid with anti-oxidation and light protection effects is mixed into solid lipid to construct a nano carrier matrix with a light protection function. Plant active ingredients with a light protection effect and an anti-oxidation effect and ergothioneine in the bolete extract are jointly loaded in a nano-structure lipid carrier, so that synergistic interaction of multiple effects is realized. The synergistic delivery system of the nanostructured lipid carrier can inhibit the photodegradation reaction of the ergothioneine and the generation of peculiar smell, improve the photostability of the ergothioneine, enhance the oxidation resistance of the ergothioneine in an acid environment, and improve the anti-aging effect.

In order to facilitate the approval and commercialization of silicasome drug delivery systems (e.g. irinotecan silicasomes) it is necessary to scale up synthesis of the drug-loaded silicasomes. In this regard, it was discovered that the synthesis protocols used for laboratory synthesis of drug-loaded silicasomes (e.g., 500 mg / batch) do not scale to large scale silicasome production, because the resulting products were too heterogeneous for use as pharmaceuticals. Accordingly, new methods are provided herein that effectively afford the large-scale production of mesoporous silica nanoparticles (MSNPs) and lipid bilayer coated MSNPs (silicasomes).

The invention belongs to the technical field of biological medicines, and particularly relates to a ferrozirconium bimetallic nano-material, a preparation method and application of the ferrozirconium bimetallic nano-material in preparation of medicines for treating breast cancer. The ferrozirconium bimetallic nano material is formed by loading a CDK4 / 6 inhibitor on a nano carrier; wherein the nano carrier is an organic framework material Zr-Fe MOF containing double metals of Zr and Fe, and the CDK4 / 6 inhibitor is preferably ribociclib. A product ZFRH for treating breast cancer is obtained based on coupling of the ferrozirconium bimetallic nano-material provided by the invention with a targeted drug such as trastuzumab. The ZFRH provides a new method for developing a nano platform with an antibody function, and realizes a treatment strategy of combined anti-tumor of trastuzumab and a CDK4 / 6 inhibitor. As a safe and effective tumor treatment strategy, the ZFRH has huge clinical transformation potential.

The invention relates to technical development and application of new special medical food on the basis of a new food raw material framework, and belongs to the technical field of special medical purpose formula food (FSMP). According to the series of foods, new food raw materials (euglena, haematococcus pluvialis and ampelopsis grossedentata leaves) are taken as cores, medicinal and edible components (lotus leaves, haws and mulberry leaves) and marine active substances (fucoidan polysaccharides and chitin) are combined, functional factors are extracted through a prehydrolysis SBE technology and a biological enzymolysis technology, and the bioavailability is improved by adopting a nano-carrier technology. The product comprises a total nutrient base material (20-30% of protein and 15-25% of dietary fiber), functional components (10-15% of a new food raw material extract and 8-12% of a traditional Chinese medicine extract) and a metabolism regulator (conjugated linoleic acid and L-carnitine). Clinical tests show that after an obese patient takes 400 kcal of the replacement meal every day, the weight of the obese patient is reduced by 10.2 kg on average (4.8 kg of a control group) 12 weeks later, and the body fat rate is reduced by 16.3%. The product supports line iteration (such as powder, tablets and liquid food) through modular formula design, and fills the application blank of new food raw materials in special medical food.

A cage-like nanocarrier for targeted delivery of siRNA, and a preparation method therefor and the use thereof. The preparation method comprises: (A) mutating a negatively charged or uncharged amino acid on the inner surface of a ferritin into a positively charged amino acid; and any one or more of the following steps: (B) coupling the N-terminus of the ferritin to a functional peptide having nucleic acid affinity; (C) coupling the N-terminus of the ferritin to a functional peptide promoting lysosomal escape; (D) truncating the E-helix at the C-terminus of the ferritin; (E) coupling the C-terminus of the ferritin to a functional peptide having nucleic acid affinity; and (F) coupling the C-terminus of the ferritin to a functional peptide promoting lysosomal escape. A new nucleic-acid-loaded protein nanocage carrier is constructed by means of modifying a negatively charged inner cavity of ferritin to make same positively charged. By means of electrostatic adsorption, a negatively charged siRNA can be efficiently loaded into a ferritin nanocage, thereby significantly improving the in-vivo and in-vitro delivery stability of siRNA, lysosomal escape functions, and efficacy of targeted therapy.

The invention relates to the technical field of process optimization, and discloses a preparation process optimization method and system of compound lidocaine nanogel. The method comprises the following steps: carrying out difference determination on carrier binding affinity of a lidocaine main drug and an auxiliary drug in compound lidocaine to obtain a drug feeding sequence table; carrying out surface potential pre-adjustment on the nano-carrier according to the drug feeding sequence table to obtain a drug-carrying pretreatment carrier; step-by-step drug loading is carried out according to the drug feeding sequence table based on the drug loading pretreatment carrier, and saturation data in the drug loading process is obtained; the saturation data are synchronously input into a drug loading process regulation and control system for pH and temperature optimization, and multi-component balanced drug loading particles are obtained; layered gel cross-linking is performed according to the multi-component balanced drug-loading particles to obtain nanogel, drug-loading stability and release consistency verification is performed on the nanogel, cross-linking optimization process parameters are generated, and the adaptability and the drug-loading efficiency of the drug-loading process are improved.

The invention discloses a multi-epitope peptide of novel coronavirus, a vaccine as well as a preparation method and application of the multi-epitope peptide, and belongs to the technical field of preparation of polypeptide vaccines. The multi-epitope peptide of the novel coronavirus not only contains T cell epitopes, but also contains B cell epitopes, and can induce humoral immunity and cellular immunity responses of an organism at the same time; the vaccine comprises epitopes from four antigenic proteins of novel coronavirus, and can induce comprehensive immune response. According to the multi-epitope peptide vaccine based on the novel coronavirus, the nano-carrier KFE8 serves as a delivery carrier, different antigen epitopes of the same pathogen or antigen epitopes of different pathogens can be presented in a mixed mode, safety is good, large-scale rapid preparation is achieved in an emergency state, and the vaccine is suitable for large-scale popularization and application. The coronavirus vaccine is a novel coronavirus universal vaccine which can cover various pathogen subtypes and induce broad-spectrum and long-term immune effects.

The application provides a multifunctional nano particle for targeting abdominal aortic aneurysm and a preparation method and application thereof, and belongs to the field of nanobiomedicine, wherein polyphenol oxidation self-polymer nanoparticles are used as carriers, doxycycline is loaded, and a targeting ligand cRGD is modified on the surface of the nano carrier; the neovasculature in the media and adventitia of AAA sites helps accumulation of the nanoparticles in the abdominal aortic aneurysm, and the integrin αν3 beta receptor highly expressed on the surface of the diseased cell membrane can recognize the cRGD with high affinity, and then mediate the targeted endocytosis of the nanoparticles; after intravenous injection, the nanoparticles can be effectively enriched in the lesion site and achieve long-term retention, and can release DC in response to the high ROS level in the tumor microenvironment; the drug is rapidly released to take effect, and the non-specific toxic side effects are reduced; the free radical scavenging capacity of the nanoparticles can be synergized with the DC activity to treat AAA through multiple mechanisms such as anti-inflammatory, antioxidant, macrophage repolarization promotion, anti-apoptosis and calcification inhibition, and MMPs inhibition.

The present invention provides a method for suppressing a target gene involved in the Wnt / β-catenin signaling pathway in target cells expressing a receptor capable of binding an extracellular Wnt ligand and an intracellular Wnt effector. The method comprises providing nanocarriers and binding the nanocarriers to the target cells and / or internalizing of the nanocarriers into the target cells, whereby the target gene is suppressed in the target cells. In each nanocarrier, an antibody specific for the receptor, a siRNA specific for the effector, or a combination thereof may be attached to a nanoparticle. The target cells may be in a subject. The method may further comprise reducing disease burden and / or inducing stabilization of a disease in the subject. Also provided are methods for synthesizing the nanocarriers.

This invention belongs to the field of drug carrier technology and discloses a brain-targeted nanocarrier, its preparation method, and application. The primary raw materials for preparing the brain-targeted nanocarrier include PLGA-PEG-NHS, D-mannosamine, and a sulfide donor. This brain-targeted nanocarrier utilizes the dual targeting effects of mannose and sulfide to not only target the brain but also effectively improve blood-brain barrier permeability. With its high targeting and high permeability, it can be used as a carrier to effectively deliver therapeutic drugs to the brain to exert therapeutic effects, and has promising application prospects.

The invention belongs to the technical field of biomedical detection, and particularly discloses a nucleic acid aptamer for specifically recognizing serum alpha fetoprotein and application of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer comprises a sequence as shown in SEQ ID No.1, or has more than 90% of homology with the sequence as shown in SEQ ID No.1, or one or more nucleotides are deleted or added in the sequence as shown in SEQ ID No.1. The nucleic acid aptamer can realize specific efficient recognition of AFP protein, also shows extremely high selectivity in a complex serum sample environment, eliminates non-specific adsorption interference of homologous protein and other serum interference protein, greatly reduces the false positive rate, and improves the accuracy and specificity of clinical detection. And the material constructed by directionally functionalizing the aptamer on the surface of the magnetic nano-carrier has rapid magnetic response performance, separation and enrichment of target protein can be realized within seconds, and the clinical diagnosis time is greatly shortened.

The application discloses an iron / copper-porphyrin nanoparticle and a preparation method and application thereof, and the nanoparticle is prepared through the following steps: adding iron chloride, copper chloride, tetra(4-carboxyphenyl)porphyrin and benzoic acid into a reaction solvent N,N-dimethylformamide solution, uniformly stirring at room temperature, and reacting at high temperature; and after the reaction is completed, the iron / manganese-porphyrin nanoparticle is obtained through centrifugation and washing. The iron / manganese-porphyrin nanoparticle is prepared by using a brand-new method, the nanoparticle can be degraded in a glutathione solution and release iron ions and copper ions, is used for catalyzing hydrogen peroxide to be converted into a hydroxyl radical (•OH), and tetra(4-carboxyphenyl)porphyrin can generate singlet oxygen (O2) under 660 nm laser irradiation, and is expected to become a good nano-carrier for accelerating photodynamic therapy-chemical kinetic therapy. 1 O2).

The invention discloses a vaccine platform for in-vivo in-situ generation of CAR-T cells as well as a preparation method and application of the vaccine platform, and belongs to the technical field of biological medicines. In order to solve the problems that in the prior art, a CAR-T cell therapy is complex in preparation process, high in cost, poor in durability and poor in curative effect in clinical application of solid tumors, the invention provides a vaccine platform for generating CAR-T cells in vivo in situ, and the vaccine platform is composed of an mRNA delivery vector for expressing antigens; the carrier is an mRNA (messenger Ribonucleic Acid) nano-carrier of which the surface is modified with a specific antigen and which loads and encodes CAR; the vaccine platform is a universal mRNA delivery platform, provides a new universal strategy for enhancing the clinical effect of a CAR-T cell therapy, and has a wide application prospect.

A self-assembling composition includes molecules of a first polymer, molecules of a positively charged second polymer, and a nucleic add. First pendant groups conjugated to pendant amine group of the first polymer include a hydrophilic polymer. Second pendant groups conjugated to the pendant amine groups include a hydrophobic group. Third pendant groups conjugated to the pendant amine groups include a group selected from a nucleoside and a nucleoside analog. Fourth pendant groups conjugated to the second polymer via pendant amine groups thereof include the hydrophilic polymer. Fifth pendant groups are conjugated to the amine groups of the second polymer include the hydrophobic group. The second polymer also includes repeat units wherein the pendant amine groups are positively charged in vivo.

The invention provides a nucleic acid molecule. The nucleic acid molecule comprises a nucleic acid sequence as shown in SEQ ID NO.2. The invention also provides a polypeptide which is encoded by the nucleic acid molecule. The invention further provides a recombinant plasmid, and the recombinant plasmid comprises the nucleic acid molecule. The invention also provides a preparation method of the recombinant protein. The preparation method comprises the following steps: optimizing an HBc gene into a nucleotide sequence comprising SEQ ID NO.2; constructing a recombinant plasmid based on the nucleic acid sequence; the recombinant plasmid is transfected into an HEK293 cell for expression; the cell supernatant is collected for purification of the recombinant protein. The HBc gene is optimized according to the codon preference of the HEK293 cell, and the codon adaptation index (CAI) value of the HBc gene is increased to 0.930. The expression system established by the invention has the remarkable characteristics of high efficiency and low pollution, not only overcomes the defects of the traditional expression system, but also provides a brand new strategy and technical support for efficient production of vaccines and development of nano-carriers.

This invention relates to the fields of pesticide formulations and pesticide pest control, specifically to a composite nano-slow-release suspension of emamectin benzoate and chlorantraniliprole, its preparation, and application. The nano-slow-release suspension comprises emamectin benzoate, chlorantraniliprole, a nanocarrier, a wetting agent, a dispersant, a synergist, a thickener, an antifoaming agent, an antifreeze, a preservative, and water. The nanocarrier is an NX-CMCS & MOF composite nanocarrier. Spraying this composite nano-pesticide onto crops improves the adhesion and penetration of the pesticide on leaves, enhances its resistance to rain washout, and effectively kills major crop pests such as bollworms, diamondback moths, cowpea thrips, and rice planthoppers. Compared with traditional pesticide formulations, it has a significant effect of reducing dosage and increasing efficacy, and has broad application prospects in the field of agricultural pest control and promoting green agriculture.

The invention provides chlorine dioxide nano-carrier encapsulation integrated equipment and an operation method thereof, and belongs to the technical field of mechanical equipment. The problems that the manufacturing procedure cannot be completed in the one-time conveying process in the existing nano-carrier production, and the production time is long are solved. According to the chlorine dioxide nano-carrier encapsulating integrated equipment and the operation method thereof, the integrated equipment comprises mixing and stirring equipment and encapsulating equipment, the mixing and stirring equipment is arranged on the left side of the encapsulating equipment, and raw materials are mixed, stirred and processed through the mixing and stirring equipment and then encapsulated through the encapsulating equipment. The method has the advantage that the machining efficiency is improved.

A kind of biotin or avidin labeled fat body and its preparation method, nano-carrier system. Biotin or avidin labeled fat body is prepared using specific amount of Bio-phospholipid or avidin-phospholipid, and the prepared fat body has the advantages of high purity, small particle size, good uniformity and high stability. Based on the biotin-avidin system, a nano-carrier system is constructed, which includes the biotin labeled fat body and the avidin modified target component. The system enables the fat body to carry any interested substance, such as viral recombinant protein, antibody and other protein drugs, nucleic acid drugs or small molecule drugs, etc., so as to be used for the treatment of diseases such as vaccines, cancer, infectious diseases and metabolic diseases.

The invention discloses a tellurium-based nano material capable of efficiently inducing copper death as well as a preparation method and application of the tellurium-based nano material. The preparation method comprises the following steps: by taking water as a medium, mixing soluble copper salt and a sulfydryl-containing compound, and stirring to react, so as to obtain a Cu-S2 solution; dropwise adding the Cu-S2 solution into the TeO2 nano-carrier solution, and carrying out stirring reaction; and after the reaction is finished, carrying out solid-liquid separation, taking the solid, and resuspending with methanol to obtain the TeO2 coated Cu-S2 nano material. The preparation method is simple and rapid, large-scale production can be achieved, and the obtained TeO2 coated Cu-S2 nano material can efficiently induce cancer cells to generate copper death, is effective to various cancer cells and has potential clinical transformation application prospects.

A heterostructured electrocatalyst for carbon dioxide reduction reaction includes a lanthanide oxide nanomaterial deposited on a gold-containing nanosupport. A method of preparing the heterostructured electrocatalyst and use of the heterostructured electrocatalyst in an electrode are also addressed.

The present invention relates to a preparation method and application of a surface protein imprinted polymer relying on an enzyme cascade reaction; the surface imprinted polymer is prepared under conditions of high monomer concentration by mediating local free radical polymerization through an enzyme cascade reaction. The local free radical polymerization reaction is initiated by an initiation system consisting of GOx / HRP, acetylacetone and glucose fixed on the surface of a nanocarrier, so that the polymerization reaction proceeds around the surface of the nanocarrier. The random freedom of free radicals in the entire solution is avoided, and even at high monomer concentrations, no agglomeration phenomenon occurs during polymerization. At the same time, the method is carried out under conditions of high monomer concentration, which is conducive to the sufficient pre-assembly of functional monomers and template proteins, thereby improving the imprinting efficiency. The imprinting capacity of the synthesized nanoimprinted microspheres for lysozyme is 145.3 mg g ‑1 , the imprinting factor is 14.2.

The invention relates to a method for avoiding a human body pre-stored anti-PEG antibody and application of a terminal hydroxyl group-containing PEGylated nano-carrier in preparation of a medicine for avoiding the human body pre-stored anti-PEG antibody. The composition contains a terminal hydroxyl group-containing PEGylated nano-carrier and a nano-preparation. The terminal hydroxyl group-containing PEGylated nano-carrier and the nano-preparation are low in combination with the anti-PEG antibody pre-stored in the human body, so that the anti-PEG antibody can be prevented from being quickly cleared in the blood of the human body, and the treatment effect can be better exerted. Besides, the PEGylated nano-carrier containing terminal hydroxyl and the nano-preparation can relieve complement activation by avoiding combination with a pre-stored anti-PEG antibody in human blood, so that side effects such as clinical injection reaction and the like are relieved.

The invention discloses a dog head roundworm rTcMUCs-PLGA nano vaccine as well as a preparation method and application thereof, and the dog head roundworm rTcMUCs-PLGA nano vaccine is characterized in that the nano vaccine takes PLGA nano microspheres as a carrier and is loaded with TcMUC-2 and / or TcMUC-3 recombinant protein. Animal protection test results show that compared with a PBS and PLGA control group, the number of L3-stage larvae of the dog head roundworm in livers and lungs of mice inoculated with the rTcMUC-2-PLGA and the rTcMUC-3-PLGA in an immune group is obviously reduced, the number of L3-stage larvae of the dog head roundworm in the immune group is 55.31% and 53.61% compared with the number of L3-stage larvae of mice inoculated with the rTcMUC-2 and the number of L3-stage larvae of the mice inoculated with the rTcMUC-3 in an immune group taking saponin as an adjuvant, and after the rTcMUC-2 and the rTcMUC-3 are coated with the PLGA as a nano-carrier, the L3-stage larvae of the dog head Compared with the prior art, the PLGA nano-vaccine has the advantages that the immune frequency is reduced from three times to two times, the worm reduction rates respectively reach 65.13% and 55.9%, and the result proves that the PLGA nano-vaccine can be used as an adjuvant of recombinant protein to reduce the immune cycle and induce a host to generate better immune protection force to inhibit invasion and migration of larvae of the dog head roundworm.

The invention belongs to the technical field of particle size analysis, and discloses a laser particle size analyzer for polysaccharide nano-carrier preparation, which comprises an analyzer main body, a servo motor fixed at the bottom end in the analyzer main body, a sample cylinder fixed at the top end of the servo motor, and a refraction mechanism mounted in the analyzer main body, a protection mechanism is arranged in the analyzer body, a cleaning mechanism is installed in the analyzer body, and through cooperation of structures such as an air cylinder, an extrusion part and first connecting rods, the device can drive the extrusion part to ascend and descend by starting the air cylinder, and then the extrusion part drives the two first connecting rods and a refraction plate to rotate in the ascending and descending process; when the refraction plate rotates, light overflowing from the laser detection head can be refracted, the light is emitted to a sample again, the light makes contact with sample particles from different irradiation paths, then the measurement accuracy is improved, and therefore the purpose that the device can conveniently enlarge the sample measurement range through refraction is achieved.