Boletus concentrated powder composition lipid carrier with anti-aging effect as well as preparation method and application of boletus concentrated powder composition lipid carrier

Abstract

The invention provides a bolete concentrated powder composition lipid carrier with an anti-aging effect as well as a preparation method and application of the bolete concentrated powder composition lipid carrier. The lipid carrier is prepared from the following substances in percentage by mass: 10 to 40 percent of bolete extract, 0.2 to 0.8 percent of antioxidant, 45 to 66 percent of solid lipid, 10 to 16 percent of liquid lipid and 4.2 to 7.8 percent of surfactant. According to the bolete concentrated powder composition lipid carrier provided by the invention, liquid lipid with anti-oxidation and light protection effects is mixed into solid lipid to construct a nano carrier matrix with a light protection function. Plant active ingredients with a light protection effect and an anti-oxidation effect and ergothioneine in the bolete extract are jointly loaded in a nano-structure lipid carrier, so that synergistic interaction of multiple effects is realized. The synergistic delivery system of the nanostructured lipid carrier can inhibit the photodegradation reaction of the ergothioneine and the generation of peculiar smell, improve the photostability of the ergothioneine, enhance the oxidation resistance of the ergothioneine in an acid environment, and improve the anti-aging effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional foods, and in particular relates to a lipid carrier of a boletus concentrated powder composition with anti-aging effects, and a preparation method and application thereof. Background Art

[0002] As a high-value edible fungus, porcini mushrooms are rich in a variety of nutrients, including protein, polysaccharides, multiple vitamins, amino acids, unsaturated fatty acids, and trace minerals. They are particularly advantageous in terms of ergothioneine content. Porcini mushroom extracts contain far higher levels of ergothioneine than extracts from other edible fungi, and 10-20 times more than common edible fungi (such as shiitake and oyster mushrooms). Porcini mushrooms also contain powerful antioxidants such as glutathione. Glutathione scavenges free radicals in the cytoplasm, while ergothioneine scavenges free radicals in the nucleus and mitochondria, providing a unique resource base for their use as anti-aging ingredients.

[0003] Ergothioneine (ET) is a rare, naturally occurring amino acid that cannot be synthesized directly by the human body and, therefore, can only be obtained through the diet. As a potent antioxidant, ergothioneine can effectively neutralize free radicals, reducing oxidative stress damage to the skin and other body tissues, thereby slowing the aging process and reducing the formation of skin wrinkles by protecting cells and tissues. However, literature research has shown that ergothioneine's free radical scavenging ability decreases by 54% in acidic environments below pH 4. Furthermore, ergothioneine is easily oxidized and decomposed by light, producing an unpleasant odor. These shortcomings significantly limit its application in functional foods, especially since oral ergothioneine passes through gastric acid, and the acidic nature of the beverage system in typical liquid beverage formulations reduces ergothioneine's antioxidant properties and weakens its anti-aging efficacy.

[0004] While traditional encapsulation techniques (such as cyclodextrin inclusion and microencapsulation) can partially improve stability, they suffer from disadvantages such as low encapsulation efficiency, complex processes, and high costs. Therefore, it is necessary to develop a green, efficient, targeted delivery system that can simultaneously enhance the acid and photostability of ergothioneine, improve its bioavailability, and enhance its sustained release, thus providing technical support for the development of stable and efficient oral cosmetic products. Summary of the Invention

[0005] The purpose of the present invention is to provide a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, so as to overcome the above-mentioned technical problems existing in the prior art.

[0006] Another object of the present invention is to provide a method for preparing a lipid carrier of a boletus concentrated powder composition having anti-aging effects, thereby improving the stability of the system.

[0007] Another object of the present invention is to provide an application of a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy to achieve better anti-aging effects.

[0008] To this end, the technical solutions provided by the present invention are as follows: A lipid carrier of a boletus concentrated powder composition with anti-aging efficacy is composed of the following substances in percentage by mass: 10-40% of a boletus extract, 0.2-0.8% of an antioxidant, 45-66% of solid lipids, 10-16% of a liquid lipid, and 4.2-7.8% of a surfactant. The bolete extract has an ergothioneine content of greater than or equal to 5% and a glutathione content of greater than or equal to 1%.

[0009] The antioxidant is one of rutin, quercetin, ferulic acid and chlorogenic acid.

[0010] The solid lipid is lecithin or glyceryl monostearate.

[0011] The liquid lipid is vitamin E acetate or vegetable oil, and the vegetable oil is one of corn oil, linseed oil, and grapeseed oil.

[0012] The surfactant is one of Tween 20, Tween 60 and Tween 80.

[0013] A method for preparing a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy comprises the following steps: Step 1) dissolving the formulated amount of antioxidant in anhydrous ethanol, then heating the formulated amount of solid lipid and liquid lipid at 60-85°C until completely melted, stirring and dispersing them evenly to obtain an organic phase; Step 2) dissolving the formulated amount of boletus extract in water and filtering to remove impurities, adding the formulated amount of surfactant and mixing evenly, and heating to 60-85° C. to obtain an aqueous phase; Step 3) adding the organic phase to the continuously stirred aqueous phase, continuing stirring to remove the anhydrous ethanol, and then performing high-speed shearing and high-pressure homogenization to obtain colostrum; Wherein, the stirring speed is 300-1000r / min, and the stirring time is 5-20min; Step 4) ultrasonically treating the colostrum to further reduce the droplet size to obtain a dispersion; Among them, the ultrasonic power is 120-900W, and the ultrasonic time is 5-10min; Step 5) Rapidly cooling the dispersion to room temperature to solidify the lipids; Step 6) spray drying to obtain the boletus concentrated powder composition lipid carrier powder.

[0014] Step 3) The medium-high speed shearing speed is 6000-15000 rpm, and the shearing time is 2-6 minutes; the high-pressure homogenization pressure is 10-20 MPa, and the number of times is 1-3 times.

[0015] The invention discloses an application of a lipid carrier of a boletus concentrated powder composition having anti-aging effects in the preparation of a health-care food having anti-aging effects.

[0016] The mass percentage of the lipid carrier of the boletus concentrated powder composition to the solid content of the health food is 5%-100%, and the health food is in the form of solid, liquid, tablet or capsule.

[0017] The beneficial effects of the present invention are: The present invention constructs a kind of coordinated delivery system based on nanostructured lipid carrier, by co-encapsulating ergothioneine in bolete extract with specific antioxidant, realizes the synergistic enhancement of multiple effects.Wherein liquid lipid not only as carrier structure component, also has antioxidant and light protection ability, and constructs the nanocarrier system with light protection function together with solid lipid.This nanostructured lipid carrier can overcome the shortcomings that other carriers such as nanoemulsion, micelle and liposome preparation process are complicated, encapsulation efficiency is low, drug loading is low, and stability is poor.

[0018] The present invention particularly selects the antioxidant with light protection characteristics, such as quercetin, rutin, ferulic acid and chlorogenic acid, can absorb ultraviolet light so as to play light protection.Not only can the photostability of thioneine itself be improved, and by carrying out thioneine and antioxidant to be totally encapsulated, a collaborative antioxidant system is formed, wherein liquid lipid, antioxidant and thioneine synergistically enhance the antioxidant capacity of system in acidic environment, promote its anti-aging effect.

[0019] The invention provides a solution for the future application of boletus in health foods and health products, and has broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is the photostability curve of ergothioneine of different samples; Figure 2 is a bar graph of DPPH scavenging ability of ergothioneine of different samples; Figure 3 This is a bar graph of the average improvement rate of wrinkles in clinical trial subjects; Figure 4 This is a bar graph of the highest wrinkle improvement rates among clinical trial subjects; Figure 5 This is a before-and-after comparison of wrinkle improvement on the face of a clinical trial participant; Figure 6 This is a before-and-after comparison of wrinkle improvement on the left face of a clinical trial participant; Figure 7This is a before-and-after comparison of wrinkle improvement on the right face of a clinical trial subject. DETAILED DESCRIPTION

[0021] The following describes the embodiments of the present invention through specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0022] Exemplary embodiments of the present invention will now be described with reference to the accompanying drawings. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein. These embodiments are provided so as to provide a thorough and complete disclosure of the present invention and fully convey the scope of the present invention to those skilled in the art. The terminology used in the exemplary embodiments shown in the accompanying drawings is not intended to limit the present invention. In the accompanying drawings, identical elements are denoted by the same reference numerals.

[0023] Unless otherwise specified, the terms used herein (including technical and scientific terms) have the meanings commonly understood by those skilled in the art. Furthermore, it is understood that terms defined in commonly used dictionaries should be understood to have meanings consistent with the context of their relevant fields and should not be interpreted as idealized or overly formal.

[0024] Example 1 The invention provides a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy. The lipid carrier is composed of the following substances in percentage by mass: 10-40% of a boletus extract, 0.2-0.8% of an antioxidant, 45-66% of solid lipids, 10-16% of a liquid lipid, and 4.2-7.8% of a surfactant. The ergothioneine content in the bolete extract is greater than or equal to 5%, and the glutathione content is greater than or equal to 1%. Wherein, the extraction method of the bolete extract is prior art.

[0025] The antioxidant is one of rutin, quercetin, ferulic acid and chlorogenic acid.

[0026] The solid lipid is lecithin or glyceryl monostearate.

[0027] The liquid lipid is vitamin E acetate or vegetable oil, and the vegetable oil is one of corn oil, linseed oil, and grapeseed oil.

[0028] The surfactant is one of Tween 20, Tween 60 and Tween 80.

[0029] Principle of the present invention: Certain antioxidants such as flavonoids not only have antioxidant effects, but also absorb ultraviolet rays with a wavelength of 200-400 nm. Among them, rutin or quercetin are used in combination with titanium dioxide and zinc oxide respectively to have a synergistic sunscreen effect. The molecular structure of plant-derived phenylpropanoid compounds contains phenolic hydroxyl groups, which makes them have strong absorption of ultraviolet rays. Among them, ferulic acid and chlorogenic acid can also be synergistically enhanced when compounded with sunscreens. Therefore, the present invention selects rutin, quercetin, ferulic acid and chlorogenic acid, which have both antioxidant efficacy and photoprotective effect, to be co-encapsulated with ergothioneine, which can improve the photostability of the system while enhancing the antioxidant effect.

[0030] The present invention loads ergothioneine in a bolete extract into a nanostructured lipid carrier, selects an antioxidant with a photoprotective effect and embeds the bolete extract, and simultaneously, glutathione rich in the bolete extract can synergistically enhance the effect, and vitamin E acetate and vegetable oil with antioxidant and photoprotective effects are used as liquid lipid sources in the nanostructured lipid carrier, which can synergistically enhance the photostability of ergothioneine and improve the antioxidant capacity of the composition in an acidic environment, thereby enhancing the anti-aging effect.

[0031] Example 2 Based on Example 1, this example provides a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, which is composed of the following substances in the following mass percentages: 10% boletus extract, 0.2% antioxidant, 66% solid lipid, 16% liquid lipid, and 7.8% surfactant. Preparation process: Step 1) Weigh 0.02 g of quercetin and dissolve it completely in anhydrous ethanol. Add 6.6 g of lecithin and 1.6 g of vitamin E acetate and heat at 75° C. until completely melted to obtain an organic phase.

[0032] Step 2) Dissolve 1 g of boletus extract in distilled water, filter to remove impurities, add 0.78 g of Tween 80, mix well, and heat to 70° C. to obtain an aqueous phase.

[0033] Step 3) The organic phase was slowly added to the aqueous phase which was continuously stirred at 400 rpm and the stirring was continued for 15 min to remove the anhydrous ethanol.

[0034] Step 4) The mixed solution was sheared at 6000 rpm for 3 minutes and homogenized twice at a pressure of 10 MPa to obtain colostrum.

[0035] Step 5) The colostrum is ultrasonically treated at a power of 500 W for 6 minutes to obtain a dispersion.

[0036] Step 6) The dispersion was then placed in an ice bath and cooled to room temperature, and spray-dried to obtain the boletus concentrated powder composition lipid carrier powder A1.

[0037] In this embodiment, the antioxidant is quercetin, the solid lipid is lecithin, the liquid lipid is vitamin E acetate, and the surfactant is Tween 80.

[0038] Example 3 Based on Example 1, this example provides a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, which is composed of the following substances in the following mass percentages: 30% boletus extract, 0.6% antioxidant, 51.2% solid lipid, 12.8% liquid lipid, and 5.4% surfactant. Preparation process: Step 1) Weigh 0.06 g of quercetin and dissolve it completely in anhydrous ethanol. Add 5.12 g of lecithin and 1.28 g of vitamin E acetate and heat at 75° C. until completely melted to obtain an organic phase.

[0039] Step 2) Dissolve 3 g of boletus extract in distilled water, filter to remove impurities, add 0.54 g of Tween 80, mix well, and heat to 75° C. to obtain an aqueous phase.

[0040] Step 3) The organic phase was slowly added to the aqueous phase which was continuously stirred at 600 r / min and the stirring was continued for 15 min to remove the anhydrous ethanol.

[0041] Step 4) The mixed solution was sheared at 12000 rpm for 3 minutes and homogenized twice at a pressure of 15 MPa to obtain colostrum.

[0042] Step 5) The colostrum is ultrasonically treated at a power of 800 W for 8 minutes to obtain a dispersion.

[0043] Step 6) The dispersion was then placed in an ice bath and cooled to room temperature, and spray-dried to obtain the boletus concentrated powder composition lipid carrier powder A2.

[0044] In this embodiment, the antioxidant is quercetin, the solid lipid is lecithin, the liquid lipid is vitamin E acetate, and the surfactant is Tween 80.

[0045] Example 4 Based on Example 1, this example provides a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, which is composed of the following substances in the following mass percentages: 40% boletus extract, 0.8% antioxidant, 45% solid lipid, 10% liquid lipid, and 4.2% surfactant. Preparation process: Step 1) Weigh 0.08 g of quercetin and dissolve it completely in anhydrous ethanol. Add 4.5 g of lecithin and 1 g of vitamin E acetate and heat at 75° C. until completely melted to obtain an organic phase.

[0046] Step 2) Dissolve 4 g of boletus extract in distilled water, filter to remove impurities, add 0.42 g of Tween 80, mix well, and heat to 75° C. to obtain an aqueous phase.

[0047] Step 3) The organic phase was slowly added to the aqueous phase which was continuously stirred at 800 rpm and the stirring was continued for 15 min to remove the anhydrous ethanol.

[0048] Step 4) The mixed solution was sheared at 12000 rpm for 5 minutes and homogenized three times at a pressure of 15 MPa to obtain colostrum.

[0049] Step 5) The colostrum is ultrasonically treated at a power of 800 W for 10 minutes to obtain a dispersion.

[0050] Step 6) The dispersion was then placed in an ice bath and cooled to room temperature, and spray-dried to obtain the boletus concentrated powder composition lipid carrier powder A3.

[0051] In this embodiment, the antioxidant is quercetin, the solid lipid is lecithin, the liquid lipid is vitamin E acetate, and the surfactant is Tween 80.

[0052] Example 5 Based on Example 1, this example provides a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, which is composed of the following substances in the following mass percentages: 30% boletus extract, 0.6% antioxidant, 51.2% solid lipid, 12.8% liquid lipid, and 5.4% surfactant. Preparation process: Step 1) Weigh 0.06 g of rutin and dissolve it completely in anhydrous ethanol. Add 5.12 g of glyceryl monostearate and 1.28 g of grape seed oil and heat at 70° C. until completely melted to obtain an organic phase.

[0053] Step 2) Dissolve 3 g of boletus extract in distilled water, filter to remove impurities, add 0.54 g of Tween 20, mix well, and heat to 70° C. to obtain an aqueous phase.

[0054] Step 3) Slowly add the organic phase to the aqueous phase which is continuously stirred at 600 rpm and continue stirring for 10 minutes to remove the anhydrous ethanol; Step 4) The mixed solution was sheared at 10,000 rpm for 4 minutes and homogenized twice at a pressure of 15 MPa to obtain colostrum.

[0055] Step 5) The colostrum is ultrasonically treated at a power of 600 W for 7 minutes to obtain a dispersion.

[0056] Step 6) The dispersion was then placed in an ice bath and cooled to room temperature, and spray-dried to obtain the boletus concentrated powder composition lipid carrier powder A4.

[0057] In this embodiment, the antioxidant is rutin, the solid lipid is glyceryl monostearate, the liquid lipid is grape seed oil, and the surfactant is Tween 20.

[0058] Example 6 Based on Example 1, this example provides a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, which is composed of the following substances in the following mass percentages: 30% boletus extract, 0.6% antioxidant, 51.2% solid lipid, 12.8% liquid lipid, and 5.4% surfactant. Preparation process: Step 1) Weigh 0.06 g of chlorogenic acid and dissolve it completely in anhydrous ethanol. Add 5.12 g of lecithin and 1.28 g of corn oil and heat at 60° C. until completely melted to obtain an organic phase.

[0059] Step 2) Dissolve 3 g of boletus extract in distilled water, filter to remove impurities, add 0.54 g of Tween 60, mix well, and heat to 60° C. to obtain an aqueous phase.

[0060] Step 3) Slowly add the organic phase to the aqueous phase which is continuously stirred at 300 r / min and continue stirring for 5 min to remove the anhydrous ethanol.

[0061] Step 4) The mixed solution was sheared at 6000 rpm for 2 minutes and homogenized once at a pressure of 10 MPa to obtain colostrum.

[0062] Step 5) The colostrum was ultrasonically treated at a power of 120 W for 5 minutes to obtain a dispersion.

[0063] Step 6) The dispersion was placed in an ice bath and cooled to room temperature, and spray-dried to obtain the boletus concentrated powder composition lipid carrier powder A5.

[0064] In this embodiment, the antioxidant is chlorogenic acid, the solid lipid is lecithin, the liquid lipid is corn oil among vegetable oils, and the surfactant is Tween 60.

[0065] Example 7 Based on Example 1, this example provides a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, which is composed of the following substances in the following mass percentages: 30% boletus extract, 0.6% antioxidant, 51.2% solid lipid, 12.8% liquid lipid, and 5.4% surfactant. Preparation process: Step 1) Weigh 0.06 g of ferulic acid and dissolve it completely in anhydrous ethanol. Add 5.12 g of lecithin and 1.28 g of linseed oil and heat at 85° C. until completely melted to obtain an organic phase.

[0066] Step 2) Dissolve 3 g of boletus extract in distilled water, filter to remove impurities, add 0.54 g of Tween 60, mix well, and heat to 85° C. to obtain an aqueous phase.

[0067] Step 3) The organic phase was slowly added to the aqueous phase which was continuously stirred at a speed of 1000 r / min, and stirring was continued for 20 min to remove the anhydrous ethanol.

[0068] Step 4) The mixed solution was sheared at 15,000 rpm for 6 minutes and homogenized three times at a pressure of 20 MPa to obtain colostrum.

[0069] Step 5) The colostrum is ultrasonically treated at a power of 900 W for 10 minutes to obtain a dispersion.

[0070] Step 6) The dispersion was placed in an ice bath and cooled to room temperature, and spray-dried to obtain the boletus concentrated powder composition lipid carrier powder A6.

[0071] In this embodiment, the antioxidant is ferulic acid, the solid lipid is lecithin, the liquid lipid is linseed oil, and the surfactant is Tween 60.

[0072] Performance testing: The following performance tests were performed on the lipid carriers A1-A6 of the concentrated hepatic bacteria powder compositions prepared in Examples 2-7.

[0073] 1. Determination of embedding efficiency Appropriate amounts of lipid carriers A1-A6 of the boletus concentrated powder composition were taken respectively, dissolved in pure water, ultrasonically treated for 30 minutes, and filtered through a 0.22µm filter membrane to determine the total content C1 of the target components (ergothioneine / quercetin / ferulic acid / rutin / chlorogenic acid) by HPLC.

[0074] Appropriate amounts of lipid carriers A1-A6 of the boletus concentrated powder composition were taken respectively, dissolved with pure water, and then centrifuged at 5000 r / min for 5 minutes. The content of the embedded target component (ergothioneine / quercetin / ferulic acid / rutin / chlorogenic acid) C2 in the supernatant was determined by HPLC.

[0075] Embedding rate (%) = C2 / C1. The embedding rates of each sample are shown in Table 1.

[0076] Table 1 Embedding rate

[0077] As can be seen from Table 1, the embedding rate of ergothioneine in the boletus concentrated powder composition lipid carrier prepared by the present invention is between 84% and 96%, and the embedding rate of quercetin / ferulic acid / rutin / chlorogenic acid is between 83% and 93%. Among them, the embedding rates of A1 ergothioneine and quercetin are the highest, followed by A2. The embedding rate of lecithin as a solid lipid will be higher than that of monostearate, and the embedding rate of vitamin E acetate as a liquid lipid will be higher than that of vegetable oil.

[0078] 2. Photostability test The prepared boletus concentrated powder composition lipid carrier A1-A6 and ratio 1, comparative example 2, comparative example 3 are mixed with an aqueous solution having an ergothioneine concentration of 1 mg / mL, are placed in a constant temperature and humidity incubator and exposed to a UV-visible composite light source for one month, and the ergothioneine content is measured by HPLC. The experimental results are shown in Figure 1 .

[0079] Comparative Example 1 (D1) is an aqueous solution of unencapsulated bolete extract, Comparative Example 2 (D2) is an aqueous solution of a physical mixture of unencapsulated bolete extract and unencapsulated quercetin; Comparative Example 3 (D3) is an aqueous solution of a physical mixture of respectively encapsulated bolete extract lipid carriers and quercetin lipid carriers, wherein the concentrations of ergothioneine and quercetin in Comparative Examples 1-3 are consistent with the concentrations of ergothioneine and quercetin in sample A2.

[0080] The preparation process of control sample 1 (D1) is as follows: weigh an appropriate amount of boletus extract and dissolve it in water to prepare an aqueous solution with the same ergothioneine concentration as A2.

[0081] The preparation process of control sample 2 (D2) is as follows: appropriate amounts of boletus extract and quercetin are weighed and dissolved in water to prepare a physical mixed aqueous solution with the same concentration as that of A2 ergothioneine and quercetin.

[0082] The preparation process of control sample 3 (D3) is as follows: (1) Weigh 5.12 g of lecithin and 1.28 g of vitamin E acetate and heat them at 75°C until completely melted to obtain an organic phase. Dissolve 3 g of boletus extract in distilled water, filter and remove impurities, add 0.54 g of Tween 80 and mix evenly, and heat to 75°C to obtain an aqueous phase. Slowly add the organic phase to the aqueous phase, which is continuously stirred at a speed of 600 r / min. Continue stirring for 15 minutes to remove anhydrous ethanol. The mixture is high-speed sheared at 12,000 rpm for 3 minutes and homogenized twice at a pressure of 15 MPa to obtain colostrum. The colostrum is ultrasonically treated at a power of 800 W for 8 minutes to obtain a dispersion. The dispersion is then placed in an ice bath to cool to room temperature and spray-dried to obtain a boletus extract lipid carrier powder.

[0083] (2) Weigh 0.06g of quercetin and dissolve it completely with anhydrous ethanol. Add 5.12g of lecithin and 1.28g of vitamin E acetate and heat at 75℃ until completely melted to obtain an organic phase. Heat 0.54g of Tween 80 to 75℃ to obtain an aqueous phase. Slowly add the organic phase to the aqueous phase that is continuously stirred at a speed of 600r / min. Continue stirring for 15min to remove the anhydrous ethanol. The mixture is sheared at 12000rpm for 3min and homogenized twice at a pressure of 15MPa to obtain colostrum. Ultrasonicate the colostrum at a power of 800w for 8min to obtain a dispersion. Then, place the dispersion in an ice bath to cool to room temperature and spray dry to obtain quercetin lipid carrier powder.

[0084] (3) Dissolving the boletus extract lipid carrier powder and the quercetin lipid carrier powder in water to prepare a mixed aqueous solution having the same concentration as that of A2 ergothioneine and quercetin.

[0085] Depend on Figure 1 As can be seen, at the 15th day and the 30th day, compared with simple quercetin and thioneine mixture, the porcini concentrated powder composition lipid carrier after embedding can significantly improve the photostability of thioneine, physical barrier can be formed to reduce light penetration by lipid matrix, and liquid lipid selects vitamin E acetate to more effectively improve the photostability of thioneine.Simultaneously, after adding the antioxidant with light protection effect, thioneine is protected by ultraviolet absorption, and now thioneine illumination retention rate is higher.Compared with other three kinds of antioxidants, quercetin can most effectively play a protective role.Wherein the porcini concentrated powder composition lipid carrier photostability prepared by embodiment 3 is the strongest.

[0086] 3. DPPH free radical scavenging experiment The prepared boletus concentrated powder composition lipid carriers A1-A6 were prepared with pure water to form a 0.1 mg / mL ergothioneine aqueous solution, and unencapsulated solid impurities were removed by centrifugation. Comparative Example 1 (D1) was an aqueous solution of unencapsulated boletus extract, Comparative Example 2 (D2) was an aqueous solution of a physical mixture of unencapsulated boletus extract and unencapsulated quercetin, and Comparative Example 3 (D3) was an aqueous solution of a physical mixture of an encapsulated boletus extract lipid carrier and a quercetin lipid carrier. The ergothioneine and quercetin concentrations in Comparative Examples 1-3 were consistent with those in sample A2.

[0087] Take 1 ml of each of the pH 3 acid solution and gastric acid simulant solution, add 3 ml of 0.1 mmol / L DPPH solution, shake thoroughly, and incubate in the dark at room temperature for 30 minutes. Measure the absorbance of the sample solution at 517 nm. Use distilled water instead of the test solution as a blank control, and use the sample solution plus anhydrous ethanol as an error.

[0088] DPPH scavenging ability (%) = [1-(Ai-Aj) / A0] * 100% Among them, A0 represents adding 1mL distilled water + 3mL DPPH reaction solution; Ai represents adding 1mL sample solution + 3mL DPPH reaction solution; Aj represents adding 1mL sample solution + 3mL anhydrous ethanol. Figure 2 shown.

[0089] Depend on Figure 2 It can be seen that the antioxidant capacity of compositions can be significantly improved by embedding mode, and vitamin E acetate and four kinds of antioxidants can collaboratively improve the antioxidant capacity of thioneine, so as to improve the anti-aging effect of bolete concentrated powder.Wherein the bolete concentrated powder composition lipid carrier free radical scavenging rate prepared by embodiment 3 is the highest.When in pH3 aqueous solution and simulated gastric acid solution, bolete concentrated powder composition lipid carrier is respectively 1.75 times and 1.93 times of bolete extract aqueous solution free radical scavenging rate.

[0090] Example 8 This embodiment provides an application of a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy in the preparation of a health food with anti-aging efficacy.

[0091] The mass percentage of the lipid carrier of the boletus concentrated powder composition to the solid content of the health food is 5%-100%, and the health food is in the form of solid, liquid, tablet or capsule.

[0092] 1.2 g of the lipid carrier A1 prepared in Example 2, 3.5 g of erythritol, 0.05 g of citric acid, 0.05 g of sodium citrate, and 0.015 g of steviol glycoside were weighed and mixed evenly, deionized water was added, and then 3 g of concentrated fruit juice was added, mixed evenly, and sterilized to prepare a boletus concentrated powder composition lipid carrier liquid beverage.

[0093] 0.4 g of the lipid carrier A2 prepared in Example 3, 2.5 g of erythritol, 0.04 g of citric acid, 0.04 g of sodium citrate, and 0.01 g of steviol glycoside were weighed and mixed evenly, deionized water was added, and then 2 g of concentrated fruit juice was added, mixed evenly, and sterilized to prepare a boletus concentrated powder composition lipid carrier liquid beverage.

[0094] 0.2 g of the lipid carrier A3 prepared in Example 4, 2 g of erythritol, 0.02 g of citric acid, 0.02 g of sodium citrate, and 0.006 g of steviol glycoside were weighed and mixed evenly, deionized water was added, and then 1.5 g of concentrated fruit juice was added, mixed evenly, and sterilized to prepare a boletus concentrated powder composition lipid carrier liquid beverage.

[0095] 0.4 g of the lipid carrier A4 prepared in Example 5, 3 g of concentrated fruit juice powder, 5 g of erythritol, 0.08 g of citric acid, 0.08 g of sodium citrate, and 0.02 g of steviol glycoside were weighed and uniformly mixed to prepare a boletus concentrated powder composition lipid carrier solid beverage.

[0096] 30 g of the lipid carrier A5 prepared in Example 6, 12.8 g of sorbitol, 5 g of microcrystalline cellulose, 1 g of concentrated fruit juice powder, 0.7 g of sucralose, and 0.5 g of magnesium stearate were weighed and uniformly mixed, and tablets were pressed using a tablet press to prepare boletus concentrated powder composition lipid carrier tablets.

[0097] 0.4 g of the lipid carrier A6 prepared in Example 7 was weighed and placed into food-grade capsule shells to prepare lipid carrier capsules of the boletus concentrated powder composition.

[0098] Clinical effect test experiment In order to compare the anti-aging effect of the lipid carrier of the boletus concentrated powder composition, a liquid beverage prepared using Example 3 as a sample was clinically tested. The specific method is as follows: (1) Test instrument: AISIA-Q2 skin tester (2) Volunteers: healthy women aged 27-45 years old, 10 people were tested.

[0099] (3) Sample and dosage: Take after breakfast and before going to bed at night, twice a day, for a period of one month.

[0100] (4) Detection indicator: wrinkles. The ratio of the total area of ​​all detected wrinkles to the area of ​​the facial focus area. The higher the quantitative percentage, the more severe the skin wrinkles.

[0101] (5) Test process: The test time was kept consistent every day (10 am). Before the test, the subjects sat quietly in a room with constant temperature and humidity for half an hour, cleansed their faces with a facial cleanser, and then took the test. Wrinkle data on the front, left, and right faces of the subjects were collected before the test and on the 15th and 30th days after the test. The experimental results are as follows: Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 and Figure 7 shown.

[0102] from Figure 3-Figure 7It can be seen that after taking the porcini concentrate powder composition lipid carrier beverage for one month, the subjects' facial wrinkles improved by an average of 13.58%, 17.94%, and 22.21% on the front, left, and right sides of the face, respectively, with the highest improvement rates reaching 28.85%, 44.44%, and 45.45%. The synergistic antioxidant effects of ergothioneine, glutathione, quercetin, and vitamin E acetate contribute to the significant anti-aging effects of the porcini concentrate powder composition lipid carrier beverage.

[0103] From the above experiments, it can be seen that the lipid carrier of the concentrated bolete powder composition can enhance the stability of ergothioneine and improve the antioxidant capacity of ergothioneine. The health food prepared from the lipid carrier of the concentrated bolete powder composition has obvious anti-aging effect.

[0104] The above examples are merely illustrative of the present invention and do not limit the scope of protection of the present invention. Any design that is identical or similar to the present invention falls within the scope of protection of the present invention.

Claims

1. A lipid carrier of a boletus concentrated powder composition with anti-aging efficacy, characterized by: The invention is composed of the following substances in the following mass percentages: 10-40% of boletus extract, 0.2-0.8% of antioxidant, 45-66% of solid lipid, 10-16% of liquid lipid and 4.2-7.8% of surfactant.

2. The lipid carrier of the boletus concentrated powder composition with anti-aging efficacy according to claim 1, characterized in that: The bolete extract has an ergothioneine content of greater than or equal to 5% and a glutathione content of greater than or equal to 1%.

3. The lipid carrier of the boletus concentrated powder composition with anti-aging efficacy according to claim 1, characterized in that: The antioxidant is one of rutin, quercetin, ferulic acid and chlorogenic acid.

4. The lipid carrier of the boletus concentrated powder composition with anti-aging efficacy according to claim 1, characterized in that: The solid lipid is lecithin or glyceryl monostearate.

5. The lipid carrier of the boletus concentrated powder composition with anti-aging efficacy according to claim 1, characterized in that: The liquid lipid is vitamin E acetate or vegetable oil, and the vegetable oil is one of corn oil, linseed oil, and grapeseed oil.

6. The lipid carrier of the boletus concentrated powder composition with anti-aging efficacy according to claim 1, characterized in that: The surfactant is one of Tween 20, Tween 60 and Tween 80.

7. The method for preparing a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy according to any one of claims 1 to 6, characterized in that: The following steps are involved: Step 1) dissolving the formulated amount of antioxidant in anhydrous ethanol, then heating the formulated amount of solid lipid and liquid lipid at 60-85°C until completely melted, stirring and dispersing them evenly to obtain an organic phase; Step 2) dissolving the formulated amount of boletus extract in water and filtering to remove impurities, adding the formulated amount of surfactant and mixing evenly, and heating to 60-85° C. to obtain an aqueous phase; Step 3) adding the organic phase to the continuously stirred aqueous phase, continuing stirring to remove the anhydrous ethanol, and then performing high-speed shearing and high-pressure homogenization to obtain colostrum; Wherein, the stirring speed is 300-1000r / min, and the stirring time is 5-20min; Step 4) ultrasonically treating the colostrum to further reduce the droplet size to obtain a dispersion; Among them, the ultrasonic power is 120-900W, and the ultrasonic time is 5-10min; Step 5) Rapidly cooling the dispersion to room temperature to solidify the lipids; Step 6) spray drying to obtain the boletus concentrated powder composition lipid carrier powder.

8. The method for preparing a lipid carrier of a boletus concentrated powder composition with anti-aging efficacy according to claim 7, characterized in that: Step 3) The medium-high speed shearing speed is 6000-15000 rpm, and the shearing time is 2-6 minutes; the high-pressure homogenization pressure is 10-20 MPa, and the number of times is 1-3 times.

9. Use of the lipid carrier of the concentrated porcini mushroom powder composition with anti-aging efficacy according to any one of claims 1 to 6 in the preparation of a health food with anti-aging efficacy.

10. Use of the lipid carrier of the boletus concentrated powder composition with anti-aging efficacy according to claim 9 in the preparation of a health food with anti-aging efficacy, characterized in that: The mass percentage of the lipid carrier of the boletus concentrated powder composition to the solid content of the health food is 5%-100%, and the health food is in the form of solid, liquid, tablet or capsule.

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