400 results about "Quercitrin" patented technology

The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.

The invention provides a gamma delta T cell preparation as well as a preparation method and application thereof, and belongs to the technical field of T cells. The liposome is prepared from the following raw materials in parts by weight: 15-20 parts of modified gamma delta T cell liposome, 1-3 parts of cell stimulating factors and 4-7 parts of culture medium freeze-dried powder, the modified gamma delta T cell lipidosome is prepared by embedding gamma delta T cells through lipidosome, coupling with acetylenic bond modified chitosan, and further mixing with MFAP4 protein and b4GALT1 protein. The culture medium freeze-dried powder is prepared by freeze-drying the culture medium in the engineering culture process of gamma delta T cells, and the cell stimulating factors are resveratrol and quercitrin. According to the gamma delta T cell preparation prepared by the invention, the survival ability and resistance of gamma delta T cells are improved, the anti-tumor activity of the gamma delta T cells is improved, and the gamma delta T cell preparation has relatively good antioxidant and anti-inflammatory effects, reduces the inhibition of inflammation on an immune system and has a very good treatment effect on pancreatic ductal adenocarcinoma.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The invention discloses gallium ion-quercetin coordination nanoparticles (GQNPs) as well as a preparation method and application thereof, specifically, quercetin, gallium nitrate hydrate and polyvinylpyrrolidone are respectively dissolved in an absolute methanol solution to prepare a quercetin solution, a gallium nitrate hydrate solution and a polyvinylpyrrolidone solution; secondly, fully mixing the quercetin solution and the polyvinylpyrrolidone solution, dropwise adding the gallium nitrate hydrate solution, and rapidly changing the color of the mixed solution from faint yellow to orange yellow in the dropwise adding process; and finally, dialyzing the orange-yellow mixed solution to remove excessive gallium ions and polyvinylpyrrolidone, thereby finally obtaining the GQNPs. The prepared GQNPs and deferiprone show excellent treatment effects on cell and living body levels, are expected to be applied to treatment of Parkinson's disease through multi-dimensional anti-ferroptosis, do not show obvious toxic and side effects in the embodiment, and are expected to be applied to further preclinical research.

The invention discloses application of a glucosyl modified quercetin derivative and a composition containing the glucosyl modified quercetin derivative. Specifically, the invention discloses an application of glucosyl modified quercetin as an anti-skin photoaging agent. According to the application disclosed by the invention, glucosyl modified quercetin, especially glucosyl modified isoquercitrin, has a remarkable effect in the aspect of resisting skin photoaging; cosmetics prepared from glucosyl modified quercetin have good anti-aging ability, and can significantly improve percutaneous water loss, skin elasticity and skin roughness.

The invention belongs to the technical field of quality control of traditional Chinese medicines, and particularly relates to a method for establishing a fingerprint spectrum of kidney-tonifying and anti-aging tablets. The establishment method of the fingerprint spectrum of the kidney-tonifying anti-aging tablet comprises the following steps: mixing the kidney-tonifying anti-aging tablet and a methanol aqueous solution, and carrying out ultrasonic treatment to obtain a test solution; reference substances in the mixed reference substance solution comprise ferulic acid, hyperoside, quercitrin, hesperidin, rosmarinic acid and salvianolic acid B; and respectively carrying out UHPLC detection on the test solution and the mixed reference substance solution, and introducing obtained chromatograms into fingerprint spectrum software for processing to obtain the fingerprint spectrum of the kidney-tonifying and anti-aging tablets. The pretreatment method is simple, and the characteristic components of the kidney-tonifying and anti-aging tablet are completely reserved; the ultra-high performance liquid chromatography is adopted, so that the precision is high, the reproducibility is good, and the stability is good; according to the fingerprint spectrum obtained by the invention, 15 common peaks are determined, so that the overall characteristics of the kidney-tonifying and anti-aging tablet can be reflected, and the quality of the kidney-tonifying and anti-aging tablet can be effectively monitored.

The invention relates to an exosome-coated phosphorus-containing dendrimer nano-composite as well as a preparation method and application thereof. The nano-composite is prepared from exosome derived from mesenchymal stem cells, a hydroxyl-terminal phosphorus-containing dendrimer and quercetin. The exosome derived from the mesenchymal stem cells is used as a carrier, so that not only are good biocompatibility and anti-inflammatory activity achieved, but also the bioavailability of the phosphorus-containing dendrimer and quercetin can be improved. The phosphorus-containing dendrimer and quercetin exert anti-inflammatory and anti-oxidation effects by promoting microglial cells to be polarized to M2 type and removing active oxygen, so that neuronal cells are protected from apoptosis. The preparation method has the advantages of being simple, easy to operate, good in biocompatibility and the like, and the provided strategy has good development prospects and application values in treatment of Parkinson's disease or other neurodegenerative diseases.

The invention belongs to the technical field of biological medicines, and particularly relates to application of quercetin combined with curcumin in preparation of an anti-allergic reaction medicine. Through a large number of researches, the inventor finds that combined administration of quercetin and curcumin with a specific concentration can effectively inhibit mast cell degranulation, and the inhibition rate of mast cell degranulation reaches 90% or more, so that I-type anaphylaxis mediated by immune globulin E is inhibited, especially systemic anaphylaxis, and the curative effect is good. The compound can be used for preparing anti-anaphylaxis drugs. The drug combination scheme provided by the invention has important significance and wide application prospect in drugs for treating or preventing anaphylactic reactions.

The invention is applicable to the technical field of biomedicine, and provides a preparation method and application of a multifunctional nano composite material. The material has good biocompatibility and can be used as a safe preparation for tumor treatment; the preparation is simple, green and pollution-free; the application of the quercetin in anti-tumor immunotherapy is expanded; the probe has excellent fluorescence / computed tomography dual-mode imaging performance, and can be used for determining tumor boundaries and realizing accurate treatment guidance; the nano-material has excellent photo-thermal and photo-thermal enhanced catalytic performance, has the advantages of being minimally invasive, efficient, small in toxic and side effect, controllable and the like, and can effectively treat primary, metastatic or recurrent head and neck squamous cell carcinoma by activating immunity; by inducing tumor cells to release 2 '3'-cGAMP and preventing the 2 '3'-cGAMP from being degraded, a cGAS-STING signal channel is efficiently activated, and finally the anti-tumor immune effect is enhanced.

The invention discloses a preparation method of linolenic acid structural ester with synergistic biological activity, and relates to the technical field of linolenic acid structural ester preparation.The preparation method comprises the steps that specific lipase is prepared through rhizopus wei, and after an SBA-15 molecular sieve is treated, the lipase is fixed to prepare a composite catalyst; the preparation method comprises the following steps: after hydroxyl protection of quercetin, esterifying quercetin with EPA to obtain quercetin-EPA ester; esterifying glycerol and linolenic acid in the presence of a composite catalyst and microwaves, and purifying to obtain an intermediate product; carrying out microwave catalytic ester exchange on the intermediate product and quercetin-EPA ester in tert-butyl alcohol through a composite catalyst to obtain a crude product; and removing impurities from the crude product by a urea adduction method, and carrying out molecular distillation graded purification, column chromatography, deodorization and filtration to obtain a final product. According to the invention, linolenic acid, quercetin and EPA are combined through molecular design to form a compound structure ester with a multi-target effect, and the linolenic acid, quercetin and EPA achieve functional complementation through a synergistic effect, so that the biological activity of the compound structure ester is enhanced.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a method for determining the content of five flavonoid components in a hypericum perforatum medicinal material. The content determination method comprises the following steps: preparing rutin, hyperoside, isoquercitrin, quercitrin and quercetin into a mixed reference solution; extracting a hypericum perforatum medicinal material by using an extraction solvent to prepare a test solution; the mixed reference substance solutions and the test solution with different volumes are subjected to high performance liquid chromatography respectively, a mobile phase A is acetonitrile, a mobile phase B is a phosphoric acid aqueous solution with the volume concentration of 0.08-0.12%, and elution gradient is adopted. The content determination method has the advantages of high sensitivity, simplicity and convenience in operation, low cost and high detection speed.

The invention discloses a rapid quantitative detection method for quantitative analysis of multi-components by single marker (QAM) of a pharmaceutical composition containing three-ingredient rhizoma polygonati decoction, and belongs to the technical field of detection and analysis. Specific components of the traditional Chinese medicinal materials in the medicine composition containing the three-component rhizoma polygonati decoction are quantified, three components of the three traditional Chinese medicinal materials are quantified, and determination is carried out under two detection wavelengths in a gradient elution mode. The detection wavelength is as follows: the 5-hydroxymethylfurfural is 270nm, the quercetin-3-O-gentiobioside and the isorhamnetin-3-O-gentiobioside are 330nm, the wave crests are appropriate in size, the 5-hydroxymethylfurfural, the quercetin-3-O-gentiobioside and the isorhamnetin-3-O-gentiobioside do not interfere with each other, the quantitative analysis of multiple components by one marker is realized, and the method is simple, convenient, rapid, accurate, low in cost, high in efficiency and beneficial to market supervision.

The invention belongs to the technical field of biological medicine, and particularly relates to an extracellular vesicle drug delivery system, a preparation method thereof and application of the extracellular vesicle drug delivery system in treatment of Alzheimer's disease. According to the invention, one or more of stilbene glucoside, emodin, kaempferol, resveratrol and quercetin are used for regulating and controlling the expression of miRNA (micro Ribonucleic Acid) related to the disease progress in the extracellular vesicles, and the result shows that one or more of miR-let-7c-5p, miR-486-5p, miR-132-3p, miR-160b and miR-129-5p is remarkably up-regulated, and one or more of miR-342-3p, miR-16-5p and miR-125b-5p is remarkably down-regulated. The regulated extracellular vesicles can be used for treating Alzheimer's disease, relieving Tau protein abnormal phosphorylation, reducing amyloid protein beta deposition, inhibiting microglial cell activation, protecting neuronal cells and relieving memory deficits.

The invention relates to a multi-targeting nanogel drug and application thereof in relieving neuroinflammation and promoting spinal cord injury repair, the multi-targeting nanogel drug comprises quercetin-loaded liposome, the quercetin-loaded liposome comprises TAT peptide and CAQK peptide, the quercetin-loaded liposome is incorporated into double-network gel, and the double-network gel is used as a drug carrier. The dual-network gel is jointly formed by a dopamine grafted hyaluronic acid polymer and methacrylated gelatin. The multi-targeting nanogel drug can improve the dissolving property of quercetin, reduce toxic and side effects, promote targeting of an injured part, and contribute to passing through a blood spinal cord barrier, so that the integrity of the blood spinal cord barrier is protected, the spinal cord microenvironment is optimized, formation of glial scars is reduced, axon regeneration is promoted, and the recovery of a motor function is enhanced.

The invention relates to the technical field of medical sutures, and particularly discloses a PDO barb suture with an antibacterial coating and a preparation method of the PDO barb suture. According to the PDO barb suture line with the antibacterial coating, a PDO mixed suture line base body with good mechanical performance is obtained by blending, spinning and weaving L-polylactic acid and poly (p-dioxanone) according to a certain proportion; hyaluronic acid is used as a gel matrix, 4-(oxirane-2-yl methoxyl) butyl acrylate is grafted to hyaluronic acid for alkenyl modification, then the hyaluronic acid is mixed with quercetin-3-O-glucoside and TCS-ZIF-8 loaded with oridonin, and the antibacterial gel coating is obtained. And finally, dipping the PDO mixed suture line matrix into the antibacterial gel coating and carrying out ultraviolet irradiation in the presence of a photoinitiator to prepare the PDO mixed suture line. The suture line prepared by the invention has the advantages of smooth coating, efficient and lasting antibacterial performance, high strength, good flexibility, small friction force and high operability.

The invention belongs to the technical field of supramolecules, and discloses a supramolecule constructed by quercetin and ergothioneine as well as a preparation method and application of the supramolecule. The preparation method of the supramolecule constructed by quercetin and ergothioneine comprises the following steps: respectively dissolving quercetin and ergothioneine in a solvent to obtain a quercetin solution and an ergothioneine solution; the preparation method comprises the following steps: adding an ergothioneine solution into a quercetin solution in an inert atmosphere, mixing, carrying out an ionizing salt formation reaction under a heating condition, concentrating, distilling, crystallizing, filtering, washing and drying to obtain the supramolecule constructed by quercetin and ergothioneine. According to the invention, quercetin is taken as an anion precursor, ergothioneine is taken as a cation precursor, the supramolecular quercetin-ergothioneine is synthesized through ionizing salt formation reaction, the supramolecular quercetin-ergothioneine has excellent biocompatibility, solubility, skin permeability and high bioavailability, a host molecular skeleton of a quercetin monomer is completely reserved, and the supramolecular quercetin-ergothioneine can be used for preparing the supramolecular quercetin-ergothioneine. Meanwhile, the quercetin and ergothioneine compound has biological activity and effects of quercetin and ergothioneine.

The invention belongs to the technical field of pest control, and discloses application of a nitrogen-containing heterocyclic compound in control of ampullaria gigas. According to the invention, a nitrogen-containing heterocyclic compound with the activity of killing ampullaria gigas is screened by taking ampullaria gigas arginine kinase as an action target, and the nitrogen-containing heterocyclic compound is N-[4-(3, 5-diphenyl-4, 5-dihydro-1H-pyrazole-1-yl) benzyl]-N-(1-hexyl-1H-benzimidazole-2-yl) amine. The nitrogen-containing heterocyclic compound is used as an active component to be prepared into a suspending agent or a granule, the suspending agent or the granule has an excellent killing effect on ampullaria gigas, and compared with an existing arginine kinase inhibitor quercetin, the nitrogen-containing heterocyclic compound is higher in activity, good in selectivity, low in toxicity to vertebrates and huge in application potential.

The invention provides an anti-aging composition as well as a preparation method, a skin care product and application thereof. The anti-aging composition is prepared from a C6-C3-C6 skeleton compound, cationic amino acid and beta-alanyl hydroxyprolyl diaminobutyric acid benzylamine. The C6-C3-C6 structural compound is natural plant extracts such as quercetin, baicalin and the like, and has excellent oxidation resistance and high safety. Cell and in-vitro experiments prove that the combined action of the three components can effectively reduce the generation of reactive oxygen species (ROS) and enhance the anti-inflammatory action, thereby delaying the cell senescence process. On the basis, carbon dioxide is injected into the anti-aging composition, so that the anti-oxidation and anti-inflammatory capacities of the anti-aging composition are further improved. In addition, the preparation process of the anti-aging composition is simple, the safety and the anti-aging performance of the anti-aging composition are evaluated through various cell experiments and human body trials, and the anti-aging composition is suitable for human skin and can be widely added into various skin care products such as face cream, emulsion and essence.

The invention discloses an HPLC (High Performance Liquid Chromatography) specific chromatogram of Xiaoyin capsules / granules as well as construction and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and chlorogenic acid, gallic acid, hydroxysafflor yellow A, albiflorin, rutin, isovitexin, 5-O-methylvisamidoside, 3, 5-di-O-caffeoylquinic acid, 4, 5-di-O-caffeoylquinic acid, benzoic acid, quercetin, paeonol, kushenone and obakunone are taken as reference substances; the characteristic chromatogram is successfully constructed for detecting different batches of Xiaoyin capsules / particles. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least 14 components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be accurately, rapidly and simultaneously performed on a plurality of characteristic components of the Xiaoyin capsules / particles, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the Xiaoyin capsules / particles.

Compositions that contain flavonoids such as hesperidin, quercetin, hesperetin, and rutin and methods of treating viral diseases such as coronavirus (e.g., SARS COV-2) infection, influenza virus infection, rhinovirus infection, and human metapneumovirus infection.

The invention belongs to the field of biological medicine, and particularly relates to functionalized drug-loaded nanoparticles as well as a preparation method and application thereof. The macromolecule coupled folic acid modified quercetin-loaded mesoporous calcium carbonate nanoparticle T (at) QU (at) CaCO3 constructed by the invention not only can be used as a'calcium bomb 'to provide calcium ions for tumor calcium overload in a tumor slightly acidic environment, but also can be used for highly loading a traditional Chinese medicine anticancer active ingredient quercetin; besides, nanoparticles are wrapped based on polymer coupled folic acid modification, the stability of mesoporous calcium carbonate can be improved, irritation of quercetin can be reduced, in-vivo circulation can be enhanced, tumor cells can be further targeted based on folic acid modification, accumulation of drugs in TNBC cells can be promoted, and therefore the targeted delivery capacity is improved, and the purposes of effect enhancing and toxicity reducing are achieved. And a feasible scheme is provided for accurate targeted treatment of breast cancer, especially triple negative breast cancer.

The invention discloses a chitin-based fluorescent film for Zr < 4 + > detection and a preparation method thereof. The material takes a chitin film as a substrate and is chemically modified in situ by a one-pot method. The method comprises the following steps: firstly, dissolving chitin in an alkali / urea system, preparing a chitin film through a tape casting method, and washing the film with deionized water until the pH value is neutral; then, a multi-component Mannich reaction is adopted, the chitin thin film obtained after hot pressing reacts with quercetin molecules and aldehyde compounds, and the modified covalent grafted chitin-based fluorescent thin film is obtained. Through introduction of quercetin molecules, the chitin film is endowed with fluorescence performance, and meanwhile, carbonyl and polyhydroxy structures of quercetin have the capability of easily forming strong complexes with metal ions, so that the modified chitin-based fluorescent film has the characteristic of quantitatively detecting the metal ions. The preparation method disclosed by the invention accords with green chemistry, is simple to operate and stable in performance, and can be used for rapidly and highly selectively detecting zirconium (IV) ions.

The application provides a composite imprinting polymer and a preparation method and application thereof, and belongs to the field of compound extraction.The composite imprinting polymer comprises a core, a shell and a shell;the core comprises Fe3O4 and SiO2 coated on the Fe3O4;the shell comprises MOF material and imprinting polymer coated on the MOF;raw materials for preparing the imprinting polymer comprise a template molecule, a functional monomer, a crosslinking agent and an initiator;the template molecule is quercetin;the functional monomer comprises two of methyl methacrylate, styrene, 4-vinylpyridine and acrylamide; and the crosslinking agent comprises ethylene glycol dimethacrylate.The material has the triple advantages of magnetic rapid separation, high specific surface area of MOF and specific recognition of molecular imprinting, and exhibits high adsorption capacity and excellent selectivity for quercetin.

The invention relates to the technical field of traditional Chinese medicines, and discloses application of an active ingredient of shiny-leaved yellowhorn leaves in preparation of a medicine for treating hyperuricemia. The xanthoceras sorbifolia leaf active ingredients comprise quercitrin, rutin and myricetin; the content of quercitrin is 0.2508 mg / g to 0.6719 mg / g, the content of rutin is 0.6707 mg / g to 1.5831 mg / g, and the content of myricetin is 0.8002 mg / g to 3.4858 mg / g. According to the invention, myricetin, quercitrin and rutin are determined as core effective substances of shiny-leaved yellowhorn leaves for treating hyperuricemia for the first time; the multi-target and multi-channel action mechanism is disclosed; meanwhile, network pharmacological analysis shows that the three components have high connectivity in a network and jointly act on key targets such as XOD, ACE, AKR1B1 and TNF, it is prompted that the three components do not act singly, but play a treatment role through multi-target and multi-channel cooperation, the traditional'single target-single drug 'thinking is changed into a'multi-component-multi-target-multi-channel' system regulation mode, and the treatment effect is improved. A new thought is provided for comprehensive treatment of the hyperuricemia.

The invention discloses a gingko GbDRF2 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbDFR2 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbDFR2 gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbDFR2 gene is shown as SEQ ID NO.2. A GbDFR2 overexpression vector is constructed and nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the contents of 9 flavonoid substances such as sakuranetin and 3, 7-dimethyl quercetin are increased, and the contents of 10 metabolites such as naringin and chalcone are decreased. The invention discloses a regulation effect of the ginkgo GbDFR2 gene in synthesis of flavonoid compounds, and provides a theoretical basis and a molecular tool for improving plant secondary metabolites through genetic engineering.

The invention discloses hydrogel for remodeling an aging microenvironment and promoting rotator cuff injury repair and a preparation method of the hydrogel. The hydrogel comprises ordered hydroxyapatite super-long nanowire / gelatin-hyaluronic acid composite hydrogel and quercetin loaded in the ordered hydroxyapatite super-long nanowire / gelatin-hyaluronic acid composite hydrogel. According to the ordered hydroxyapatite ultra-long nanowire (AHNW) / gelatin-hyaluronic acid (GH) hydrogel compound loaded with Quercetin, the repairing capacity of bone marrow-derived mesenchymal stem cells (BMSCs) and tendon-derived stem cells (TDSCs) is improved by remodeling an aging microenvironment, so that tendon-bone healing is promoted, the integration effect is improved, and the application prospect is wide. A new treatment strategy is provided for the patient with the rotator cuff injury, especially the patient with the osteoporosis.

The invention discloses oral multi-effect analgesic and anti-inflammatory gel and a preparation method thereof, and belongs to the technical field of medical gel. The gel is prepared from mussel mucin grafted hydroxypropyl chitosan, a poly (N-isopropylacrylamide)-co-acrylic acid copolymer, quercetin-zinc coordination nanoparticles, a local anesthetic compound wrapped by temperature-sensitive lipidosome, a gamma-polyglutamic acid-catechin gel matrix, aloe polysaccharide, dipotassium glycyrrhizinate, and sodium hyaluronate and potassium sorbate which can be selectively added, wherein the local anesthetic compound is prepared from the mussel mucin grafted hydroxypropyl chitosan, the poly (N-isopropylacrylamide)-co-acrylic acid copolymer, the quercetin-zinc coordination nanoparticles, the temperature-sensitive lipidosome, the gamma-polyglutamic acid-catechin gel matrix, the aloe polysaccharide and the dipotassium glycyrrhizinate. During preparation, part of the components and water are stirred to obtain a mixed solution, and then the other components are added and stirred into gel. According to the gel, the leakage rate of local anesthetics is reduced by stabilizing the liposome through the zinc ions, the quercetin adjusts the phase transition temperature of the liposome to realize preferential drug release at the pain part, the mussel mucin and the copolymer form a reversible hydrogen bond network to enhance the mucous membrane adhesive force, and the inflammatory environment triggers the gel permeation drug release, so that the gel has the advantages of multiple-effect synergy, strong adhesion and the like; the problems that existing oral gel is short in analgesia time and insufficient in anti-inflammation can be effectively solved.

The invention provides a method for treating AR negative TNBC through combination of quercetin and enzalutamide, the quercetin up-regulates the AR expression level by inhibiting a high-expression solute carrier SLC7A5, so that tumor cells which are not sensitive to enzalutamide originally obtain drug sensitivity again; the combined use of an AR antagonist enzalutamide (1-80 [mu] M) can cooperatively block an AR signal channel and significantly inhibit cell proliferation (the inhibition rate of drug combination is 70%, Plt, 0.01 higher than that of a single drug). In-vitro experiments prove that the scheme has a synergistic effect (the effect is optimal when the mass ratio is 1: 1-5: 1) in MDA-MB-231 cells, and an animal model shows that the tumor volume inhibition rate reaches 70% or above. Safety evaluation shows that the drug combination does not cause abnormity of serum biochemical indexes (ALT / AST / BUN / CREA) or damage of main organs and tissues. The invention further provides a preparation method of an oral preparation (tablets / capsules / nanoparticles) containing quercetin (50-500 mg / day) and enzalutamide (40-160 mg / day), and a new strategy is provided for reversing AR-TNBC drug resistance.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to hovenia dulcis thunb powder with a hangover alleviating effect and a preparation method thereof. Comprising the following steps: (1) mixing hovenia dulcis thunb powder and water to obtain a mixed solution; (2) adding a compound enzyme into the mixed solution for enzymolysis to obtain an enzymatic hydrolysate A; (3) adding laccase into the enzymatic hydrolysate A for enzymolysis to obtain enzymatic hydrolysate B; (4) adding tannase into the enzymatic hydrolysate B for enzymolysis to obtain enzymatic hydrolysate C; (5) adding an alcohol solvent into the enzymatic hydrolysate C for extraction, carrying out solid-liquid separation, and concentrating and drying supernate to obtain hovenia dulcis thunb powder; wherein the compound enzyme comprises cellulase, pectinase and hemicellulose. According to the method, the contents of quercetin, myricetin, dihydromyricetin and spinosin in the hovenia dulcis thunb powder can be remarkably increased.

The present invention describes a nanoarchaeosomal composition and method for delivering biomolecules comprising nanoparticulate vesicles of archaeal lipids, wherein the biomolecule is encapsulated in the vesicles formed by archaeal lipids. Further, the therapeutic drug encapsulated can be a breast cancer targeting drug, quercetin. The nanoarchaeosomal composition is colloidal in nature, stable at high temperatures, and under oxidative stress conditions. Further, exhibits higher loading efficiency and bioavailability. It also exhibits lesser cytotoxicity.