218 results about "Haematococcus" patented technology

There is provided a method of separating free-astaxanthin selectively from green algae and, more particularly, to a method of separating free-astaxanthin selectively from Haematococcus pluvialis, the method comprising: mixing a cell culture containing Haematococcus pluvialis with an alkanic solvent and stirring, thereby obtaining an alkanic solvent extract containing astaxanthin material including free-astaxanthin and astaxanthin ester (step 1); and mixing the alkanic solvent extract with an alcohol and stirring, thereby obtaining an alcoholic extract containing free-astaxanthin (step 2). The present invention can not only maximize the efficiency of separating astaxanthin in high concentration by providing a very simple process technique capable of recovering free-astaxanthin selectively from H. pluvialis culture with high efficiency, but also realize the economical and simple operation of the process of separating free-astaxanthin by repeatedly using an alkanic solvent as a mediating solvent for extracting free-astaxanthin. Such a separation method can be used in extracting and separating a carotinoid analogue containing astaxanthin from a useful microalgae culture in addition to Haematococcus species.

The invention discloses a composition containing a haematococcus extract and a collagen peptide. The composition is prepared from the following components in part by weight: 1 to 20 parts of haematococcus pluvialis extract, 1 to 10 parts of collagen peptide and 1 to 90 parts of safflower seed oil. The invention also discloses a preparation method for the composition. Natural astaxanthin in the haematococcus pluvialis extract has the ultrahigh oxidation resistance, ensures the normal metabolism of skin, inhibits the generation of melanin and enhances the effect of collagen; the absorption rate of the collagen peptide is between 95 and 100 percent; the collagen peptide can improve the metabolism of skin cells effectively, and can be utilized by the skin cells more easily, so that the activity of the collagen in the skin is enhanced, and the aims of preserving moisture, resisting wrinkles and nourishing the skin are fulfilled; and due to the addition of the safflower seed oil, the composition contains linoleic acid with high content and vitamin E and can prevent the oxidative deterioration of the astaxanthin, an antioxidant is not needed to be added, and the quality guarantee period can be prolonged by one time.

The invention provides a haematococcus extract preparation, and a preparation method and use thereof. A haematococcus extract soft capsule provided by the invention comprises the contents of haematococcus extract and an oleic phase, wherein the astaxanthin content of the haematococcus extract is 2 to 20 percent (w / w); and the mass-to-volume ratio (g / mL) of astaxanthin in the haematococcus extract and the oleic phase is 1-10:100. The invention also provides the method for preparing the haematococcus extract soft capsules. Experiments show that the haematococcus extract soft capsule provided by the invention has stability remarkably superior to that of the haematococcus extract and conventional products and is of great importance to the application of the astaxanthin to antioxidation, the enhancement of immunity, the suppression of tumorigenesis, the prevention and treatment of cardiovascular disease, the prevention of skin aging and the maintenance of health of eyes and the central nervous system.

The invention belongs to the technical field of feeds and in particular relates to a feed for facilitating swimming crabs to breed red cream. The feed comprises the following components in percentage by weight: 30-60 percent of fish meal, 10-30 percent of an adhesive, 1-5 percent of shrimp shell meal, 0.5-3 percent of Haematococcus algae powder, 0.2-1 percent of cassava modified starch, 0.2-1 percent of astaxanthin grease, 1-3 percent of fish oil, 2-6 percent of phagostimulant, 0.1-1 percent of carrageenan, 0.1-1 percent of xanthan gum, 0.02-0.5 percent of vitamins, 0.1-1 percent of choline chloride and 20-40 percent of water. According to the formula, the feed can provide basic nutrient elements required in the cream breeding period of the swimming crabs; particularly, as the Haematococcus algae powder and the astaxanthin grease are added to the feed, the purity of the crab cream can be effectively enhanced due to natural astaxanthin substances contained in the red globose alga powder and the astaxanthin grease, and the color of the crab cream is natural and stable; and moreover, the phagostimulant can stimulate the olfactory organ of the swimming crabs, so that the food intake of the crabs is enhanced, and the utilization rate of the feed is improved. The invention also discloses a preparation method of the feed. The feed prepared to be in a sausage type is conveniently eaten by the crabs, the feeding amount is easier to control and regulate, and a water body is not polluted by the feed.

The invention relates to a method for quickly extracting astaxanthin from haematococcus pluvialis. The method includes particular technologies of adding organic solvents into haematococcus pluvialis powder, mixing the organic solvents with the haematococcus pluvialis powder to obtain suspension liquid, feeding the suspension liquid into explosion-proof type high-pressure homogenizers and carrying out wall breaking-extraction on the suspension liquid; separating wall-broken haematococcus pluvialis powder from the solvents to obtain astaxanthin extraction liquid and haematococcus pluvialis residues; concentrating the astaxanthin extraction liquid under the protection of inert gas and removing solvent residues to obtain the astaxanthin. The method has the advantages that the effective content yield can reach 90% at least, technological processes are short, operation is simple and speedy, and industrial production can be facilitated.

The invention belongs to the technical field of biology, relates to poultry breeding and particularly relates to nutrient enhanced laying hen feed and application thereof. The nutrient enhanced mixed feed is prepared by taking common compound chicken feed as a foundation, and adding a nutrient enhancer containing rich astaxanthin, wherein the adding amount of the nutrient enhancer containing the rich astaxanthin occupies 0.1%-5.0% of the total mass of the nutrient enhanced laying hen feed. Meanwhile, the feed is used for feeding hens during an egg laying period. The feed sufficiently utilizes the property that haematococcus contains rich natural astaxanthin, and astaxanthin has strong tinting strength and a nutritional health function; a transformation ability of laying hens is effectively used for transferring the natural astaxanthin of the haematococcus and storing the natural astaxanthin into yolk, so that nutrient enhanced functional products, which can be easily and widely accepted by the public, are produced, and requirements on dietary nutrition and health of the public are met.

The invention relates to the technical field of health food, in particular to food composition containing tissue culture of saussurea involucrate and cubilose. The food composition is prepared from 7 to 8 parts of tissue culture of saussurea involucrate, 50 parts of cubilose, 1 part of haematococcus pluvialis and 0.5 part of donkey-hide gelatin peptide, and the diameter of the tissue culture of saussurea involucrate is 20 to 100 nm. The invention provides a new type of food containing tissue culture of saussurea involucrate and cubilose, and the two kinds of raw materials which are healthy are collected in a formula, the material compositions of the two are complementary, the tissue culture of saussurea involucrate is rich in total flavonoids, total polyphenols, lilacin, chlorogenic acid and other substances, and cubilose is rich in protein, which are complementary to each other to improve the body immunity, especially for women to nourish the blood and beautifying, delay senility, and relieve dysmenorrhea.

The invention discloses an antioxidant solid drink containing haematococcus pluvialis and blackcurrant powder. The antioxidant solid drink comprises the following components in parts by weight: 0.1-6.0 parts of haematococcus pluvialis, 3-10 parts of blackcurrant juice powder, 3-10 parts of cranberry juice powder, and 3-10 parts of blueberry juice powder. Through the synergistic effect and mutual promotion of antioxidant components, the antioxidant effect of the solid drink is improved, the cost of raw materials is greatly reduced, and meanwhile, the antioxidant solid drink has the functions of promoting appetite, maintaining normal activity of nerve, protecting visual function and the like.

The invention relates to a cubilose and collagen peptide beverage. The cubilose and collagen peptide beverage is characterized by being prepared from the raw materials in percentage by weight: 8-18% of fish collagen peptides, 0.1-0.6% of cubilose powder, 1-3% of a blueberry concentrated solution, 2-4% of a passion flower concentrated solution, 1-3% of an apple concentrated solution, 0.05-0.3% of inulin, 0.1-1% of tremella polysaccharide, 0.1-0.3% of cherry powder, 0.1-0.8% of red grape concentrated fruit powder, 0.1-0.3% of stachyose, 0.5-2% of elastin peptides, 0.1-0.6% of gamma-aminobutyricacid, 0.1-1% of haematococcus pluvialis, 0.03-0.07% of a food additive, 0.01-0.03% of an essence for food, and the balance of pure water. The invention further discloses a preparation method of the cubilose and collagen peptide beverage. Two major functional components, namely cubilose and collagen peptides, of the cubilose and collagen peptide beverage disclosed by the invention complement each other, the curative effect can be exerted better, the nutrition of the cubilose and collagen peptide beverage is rich, the translation speed of a taker can be effectively increased, memory of the takercan be effectively improved, reactivation of cells and generation of collagen are promoted, nourishing before delivery, after delivery and after illnesses is facilitated, and nutrients daily essential to human bodies are supplemented.

The invention belongs to the technical field of microalgae microorganisms and relates to a wall-breaking method for haematococcus pluvialis cells which are rich in a biological active substance: astaxanthin. The wall-breaking method for the haematococcus pluvialis cells provided by the invention comprises the following three steps: 1, freezing and ice-crystallizing the haematococcus pluvialis cells and dispersing the cells in a flowing liquid below 0 DEG C; 2, adding a bioactive component which protects the haematococcus pluvialis into the liquid before the ice-crystallized algae cells are broken so as to reduce a protectant which is oxidized and decomposed after wall breaking; and 3, after uniformly grinding by a colloid mill, carrying out ultra-high-pressure wall-breaking by using an ultra-high-pressure homogeneous nanometer machine. The wall-breaking process provided by the invention is carried out under a condition of low-temperature, cold chain and close away from light and breaks walls by means of the characteristic of nanometer crushing by using the ultra-high-pressure homogeneous nanometer machine and has the advantages of rapidness, efficiency and no addition of foreign matters and can be used for effectively protecting the bioactive component.

A green algal extract that contains astaxanthin at a concentration of 8 wt % or more can be obtained by cultivating an encysted green alga in a nutrient medium while supplying carbon dioxide and providing irradiation with light at a photosynthetically active photon flux input of 8000 μmol-photon / m3 / s or more, and extracting an oil component, which contains astaxanthin. A green alga that belongs to the genus Haematococcus is preferable.

The invention discloses a method for producing microcapsules of astaxanthin powder by utilizing haematococcus pluvialis. The method comprises the following steps: 1) breeding excellent algae species of haematococcus pluvialis; 2) obtaining cultivated species by enlarging the cultivation of algae species of haematococcus pluvialis; 3) culturing the cultivated species obtained in the step 2) by utilizing a large-scale factory microalgae culturing system so as to culture haematococcus pluvialis in a large scale; 4) stressing and inducing haematococcus pluvialis to accumulate a large amount of astaxanthin so as to obtain red spores of haematococcus pluvialis; 5) collecting the red spores of haematococcus pluvialis, which are obtained in the step 4), performing low-temperature drying so as to obtain the spore powder of haematococcus.

A disclosed method for extracting astaxanthin from haematococcus pluvialis comprises algae strain culture and pretreatment, mixing with glacial acetic acid and processing in an ice bath, a cell wall breaking processing combining homogenizing-machine homogenizing and liquid-nitrogen low-temperature processing, CO2 supercritical extraction of astaxanthin, and the like. According to the method, glacial acetic acid can relatively rapidly penetrate into the surface layer of haematococcus pluvialis cells under the ice bath condition; algae cells are crushed under effects of stirring centrifugal force, extrusion force and shearing force during homogenizing, and destroy on glycoprotein in cell wall structure by glacial acetic acid at the cell surface is accelerated because of the friction force among materials; and also, intervention of ice-bath glacial acetic acid, algae cell temperature is prevented from rising during homogenizing, and the physical and chemical activities of astaxanthin are furthest protected. By employing the wall breaking process, the cell wall breaking rate is up to 97.7%, the extraction rate of the astaxanthin extractive reaches 37%, the extraction rate of astaxanthin reaches 35.05%, and the purity of astaxanthin in the product is extremely high and reaches 95.25%.

Belonging to the field of microalgal biotechnologies, the invention relates to a technological method for production of a microalgal energy (biodiesel) raw material from Haematococcus sp. The method includes: inoculating single-cell Haematococcus sp. into a photobioreactor, conducting swarm cell stage culture to obtain a biomass, then induce cells to transform into non-motile cells, and then performing rapid accumulation of high value-added natural astaxanthin and bio-energy grease; and finally carrying out settling and centrifugal separation to obtain high value-added astaxanthin and the microalgal energy (biodiesel) raw material. The method provided by the invention realizes production of bio-energy raw material grease at the same time of artificial induction of single-cell Haematococcus sp. in accumulation of high value-added active substances. The development mode not only improves the comprehensive utilization efficiency of microalgal biological resources, but also brings about economic feasibility for development of microalgae biodiesel.

The invention discloses a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of (1) breaking walls of sporophore: taking fresh wet algae mud, adding 3 mol / L HCl with an amount of two times by mass of the fresh wet algae mud, stirring uniformly, stirring for 3 min at a temperature of 50 DEG C with a stirring speed of 180 times per minute, cooling rapidly, centrifuging and washing and taking the algae bodies; and (2) extracting astaxanthin: adding ethanol into the wall-broken sporophore with an amount of two times of the sporophore, oscillating for 5 min at a low temperature of 10 DEG C, centrifuging, recovering ethanol, repeating for 2 times, merging the recovered ethanol; concentrating at a low temperature and in vacuum; and recovering ethanol by evaporation, and thus the left material being astaxanthin. The method is simple and can be easily understood, is reasonable in operation, enables the haematococcus pluvialis to perform nutritive growth relatively well, is relatively fast in transformation from nutritive growth to non-nutritive growth, and accumulates relatively much haematococcus pluvialis, thereby increasing efficiency and stability of culture, transformation and accumulation and effectively preventing contamination of miscellaneous algae during a culture process. The manufactured astaxanthin is relatively low in cost.

The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell obtained from a subterranean habitat for producing the product of interest. The microalgal cell obtained from a subterranean habitat can be cultured in the dark, in light, in low nutrition, or nutrient rich conditions for at least a portion of production cycle. A combination of these conditions can be used to specifically manipulate a microalgal cell culture to produce a product of interest. The product of interest can be a water-soluble carotenoid, for example, a water-soluble carotenoid produced by culturing an algae belonging to the genus Haematococcus or a capsular exopolysaccharide produced by culturing an algae belonging to the genus Parachlorella. Compositions containing the water-soluble carotenoid, for example, as sunscreen and compositions containing the exopolysaccharide, for example, as moisturizing cream are also described.

The invention provides a haematococcus pluvialis solid dispersion, and a preparation method and applications thereof. The provided haematococcus pluvialis solid dispersion is composed of three phases namely dry algae residues prepared by subjecting haematococcus pluvialis to CO2 supercritical extraction, microencapsulated powder prepared by subjecting the haematococcus pluvialis extract obtained by CO2 supercritical extraction to microencapsulation, and a solid dispersion carrier. The invention also provides a preparation method and applications of the haematococcus pluvialis solid dispersion. The experiment results show that the astaxanthin stability, bioavailability, and anti-oxidation activity of the haematococcus pluvialis solid dispersion are obviously better than those of the cell-wall-broken haematococcus pluvialis, CO2 supercritical extract, and microencapsulated powder prepared by subjecting the CO2 supercritical extraction extract to microencapsulation.

The present invention relates to the process of regualting haemtococcus pluvialis population density and synthesizing and accumulating astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, ventilating and oxygen supplying unit, nutritious liquid compounding and temperature controlling unit and sterilizing unit; antering environment conditions to regulate haemtococcus pluvialis population density and synthesize, accumulate and collect astaxanthin; low temperature drying; etc. The natural astaxanthin is a kind of carotinoid with important physiological functions and very strong antioxidant activity.

The present invention provides a high-yielding astaxanthin mutant strain of Haematococcus pluvialis (Haematococcus pluvialis) E1 CCTCC M 207164, which uses the wild strain of Haematococcus pluvialis as material , using ultraviolet chemical compound mutagenesis, mutagenesis selection of carotenoid biosynthesis inhibitor screening. The astaxanthin content of the algal strain is 1.83 times higher than that of the original strain, the growth rate is 14% higher than that of the original strain, the physiological and biochemical characteristics are stable, and it has stable genetic properties. It is an excellent algal strain for producing natural astaxanthin and can be used as a source of industrial production. Industrial production of natural astaxanthin.

The invention relates to a method for breaking walls of Haematococcus pluvialis and extracting astaxanthin. The method comprises the following steps: (S1) uniformly mixing Haematococcus pluvialis powder with a concentrated sulfuric acid-alcohol system, stirring at 30-70 DEG C, and carrying out wall breaking for a preset time, so as to obtain an alcohol solution containing wall-broken Haematococcus pluvialis; and (S2) adding an extraction solvent into the alcohol solution containing the wall-broken Haematococcus pluvialis obtained in the step (S1), carrying out stirring extraction at 30-70 DEG C, layering, and concentrating supernate, so as to obtain an extract containing the astaxanthin. The method has the beneficial effects that the wall-breaking temperature and the extraction temperature are relatively low, reaction conditions are mild, the loss of astaxanthin is reduced, and the content of the astaxanthin in a crude extract can reach up to about 10%, so that the subsequent purification level of difficulty can be lowered, and the commercialization of the astaxanthin is promoted. Compared with existing methods, the method has the advantages that the operation steps are relatively simplified and the type and use amount of reagents are less, so that the energy consumption can be reduced, and the comprehensive cost can be lowered.

The invention discloses a method of utilizing seawater to facilitate haematococcus pluvialis to produce natural astaxanthin, and relates to the technical field of artificial cultivation of haematococcus pluvialis. The method comprises the following steps: utilizing seawater to prepare an improved culture medium of haematococcus pluvialis, adding seawater step by step to induce vegetative growth of haematococcus, cell type transformation and accumulation of astaxanthin. The method can be applied to cultivate haematococcus pluvialis at large scale at the coastal region rich in the seawater resource to produce the natural astaxanthin; compared with the conventional cultivation method of using freshwater, the method has the advantages of short production cycle, high astaxanthin content, and low material cost, and can achieve a wide industrialized application prospect.

The invention relates to a bioactive health product development technique, and aims to provide a method for promoting growth of Haematococcus pluvialis and accumulation of astaxanthin by flue gas CO2 domestication. The method comprises the following steps: carrying out domestication culture on a Haematococcus pluvialis liquid sequentially with 6 vol% high-concentration CO2 gas and 10 vol% high-concentration CO2 gas; carrying out iterative domestication culture by using coal-fired power plant flue gas to obtain a target alga strain; and culturing sequentially under the low illumination intensity of 3000-3500Lux and under the high illumination intensity of 7500-10000Lux, and carrying out centrifugation and freeze-drying on the alga solution to obtain the Haematococcus pluvialis dry alga powder. The method obviously enhances the growth rate and astaxanthin yield, wherein the growth rate is enhanced by 47% as compared with that under air conditions, and the astaxanthin content is enhanced by 25%.

The invention belongs to the biotechnical field, and relates to an astaxanthin wine, and a preparation method and a special wine bottle thereof. The method comprises the following steps: mixing Haematococcus pluvialis powder with ethanol, carrying out homogenization and wall breaking by using a high pressure, mixing the wall-broken Haematococcus pluvialis mud with an antioxidant, filling the wine bottle cap of the special wine bottle with the obtained mixture, storing wine in the bottle, and taking off the bottle cap during drinking to make the stored Haematococcus pluvialis mud be injected to the wine bottle and be mixed with the wine in order to obtain the astaxanthin wine. Compared with direct addition of the Haematococcus pluvialis mud into the wine and uniform mixing of the mud and the wine, the method in the invention guarantees that the astaxanthin is well rapidly dissolved in the wine, and can guarantee that the astaxanthin meets the shelf life needed by market circulation; the astaxanthin wine produced through using the method has a good appearance; and the designed wine bottle for containing the astaxanthin wine allows the Haematococcus pluvialis mud containing the astaxanthin to be injected to the wine during the cap opening, and allows the astaxanthin wine with the good appearance to be obtained through simple shaking, so a drinker does not implement extra operations, no process is increased during transportation and use, and the method is convenient, fast and effective.

The invention relates to the field of microorganisms, and in particular to haematococcus pluvialis ENN71 and a cultural method and application of the haematococcus pluvialis ENN71. The haematococcus pluvialis ENN71 is short in growth period, the content of natural astaxanthin is high, and the inheritance performance is stable. The haematococcus pluvialis ENN71 provided in the embodiment is Haematococcus pluvialis and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation serial number is CGMCC No.5147, and the preservation date is August 17, 2011.

The invention discloses a healthy health-preserving food formula for beautifying skins and resisting aging and a preparation method thereof, and relates to the field of healthy health-preserving foods. The formula comprises the following components in parts by weight: 2-6 parts of small molecular fish collagen, 0.005-0.015 part of fish elastin, 0.05-0.15 part of multi-petal rose powder, 0.05-0.15part of sodium hyaluronate, 0.15-0.25 part of haematococcus pluvialis, 0.1-0.25 part of yeast beta-glucan, 0.035 part of vitamin C, 0.1 part of vitamin E, 1.5-2.5 parts of freeze-dried roxburgh rose powder, and 2.5-3.5 parts of fructo-oligosaccharide. According to the invention, the homology of medicine and food is scientifically combined with new food raw materials, the traditional Chinese medicine concept is taken as the guide, both inside and outside are paid attention to, the synergistic effect and harmonious and natural beauty of the medicines are fully exerted, and the diet and the health care are organically combined so as to achieve the purpose of beautifying skins, conditioning the inside and the outside, whitening skins, removing speckles, nourishing blood, moistening skins, removing toxic substances, caring skins, delaying skin aging, resisting sunburn and resisting wrinkles.

The invention discloses a rosemary antioxidant filter capsule and an application thereof in a cigarette filter stick. A free radical scavenging agent is directly added into a cigarette and the filterstick, accordingly the stability is poor, and the activity is reduced. The rosemary antioxidant filter capsule comprises a shell and a core material, wherein the core material comprises, by weight, 2-5 parts of rosemary soluble antioxidant, 1-2 parts of a rosemary water-soluble antioxidant, 1-3 parts of haematococcus pluvialis, 2-4 parts of soluble oil and further comprises 1-2 parts of cyanobacteria and essential oil with the weight 0.2-1% of the weight of the raw materials. Compared with the prior art, the antioxidant is directly added into tobacco or a cigarette holder, and the mode that the shell wraps a capsule is used so that the oxidant can be stable and is not lost or converted after being added into the cigarette filter stick. The rosemary antioxidant, the haematococcus pluvialisand hemp seed oil are mutually permeated and fused, and have an effective adsorption effect on tar and nicotine in smoke after being mixed; suction of tar and nicotine is reduced.

The invention relates to a white fungus composite solid beverage having an anti-oxidation function and a preparation method of the white fungus composite solid beverage. The white fungus composite solid beverage is prepared from the following raw materials and components in parts by weight: 30-40 parts of white fungus ultramicro powder, 10-20 parts of fructo-oligosaccharide, 2-8 parts of inulin, 0.02-0.08 part of haematococcus pluvialis, 0.02-0.08 part of euglena, 0.02-0.08 part of a grape seed extract, 40-50 parts of sorbitol, 0.02-0.08 part of L-carnitine, and 0.02-0.08 part of vitamin C. The preparation method comprises the following steps: a step I, preparing the white fungus ultramicro powder; a step II, blending materials; a step III, performing mixing and blending; a step IV, performing granulation; and a step V, performing sterilization, performing cooling, and performing packaging. According to the white fungus composite solid beverage and the preparation method thereof disclosed by the invention, the problem of absorption of nutrients from the white funguses in the prior art is effectively solved, and the cost problem of a high-quality white fungus beverage is solved; and besides, the white fungus composite solid beverage disclosed by the invention has various efficacies of resisting oxidation, resisting ageing, enhancing immunity of organisms and the like.

Compositions and methods for indirectly reducing incidence of fungal pathogen infection in a plant are disclosed, wherein the fungal pathogen may be Botrytis, Macrophomina, Rhizoctonia, Sclerotinia, or Verticillium. The compositions comprise pasteurized microalgae cells in an amount effective to reduce incidence of the fungal pathogen in a plant compared to a substantially identical population of untreated plants. The composition treatment can include at least one microalgae such as Chlorella, Aurantiochytrium acetophilum HS399, Galdieria, Scenedesmus, Haematococcus, Isochrysis, Spirulina or any combination thereof. Where the composition comprises a combination of a first microalgae and a second microalgae, the composition may consist of a ratio of 25:75, 50:50: or 75:25.

Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.