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95 results about "Haematococcus" patented technology

Haematococcus is a genus of algae in the family Haematococcaceae. Members of this group are the common cause for the pink color found in birdbaths.

Plant composition with skin anti-aging activity and preparation thereof

The invention discloses a plant composition with skin anti-aging activity and a preparation thereof. The plant composition is prepared from the following active ingredients in percentage by weight: 84.0 to 89.0 percent of haematococcus pluvialis extract, 8.0 to 12.0 percent of lithospermum oil, 0.5 to 0.8 percent of melaleuca alternifolia leaf oil, 0.5 to 1.0 percent of hydrolyzed olive leaf extract, 0.1 to 0.5 percent of rhodiola rosea root extract and 1.2 to 2.5 percent of bitter orange leaf oil. The plant composition with the skin anti-aging activity is used as an effective component, other auxiliaries are added, and emulsion, cream or essence oil is prepared. According to the plant composition and the preparation, the fermented camelina seed oil, the fermented safflower seed oil, the fermented meadowfoam seed oil and the like are used as solvents for extracting active components in haematococcus pluvialis and are also used as functional components and matrixes of the preparation, and compared with application of original plant oil, the plant composition and the preparation have better skin feeling and biological activity; the extraction rate of active ingredients in haematococcus pluvialis is higher, and transdermal absorption of the active ingredients is facilitated.
Owner:HENAN NAPU BIOTECHNOLOGY CO LTD +1

Haematococcus pluvialis and application thereof

According to the haematococcus pluvialis strain and the application thereof, cell wall breaking operation can be carried out more easily when astaxanthin is extracted, astaxanthin can be extracted more effectively, and the cost of natural astaxanthin is reduced. The haematococcus pluvialis RMC16 strain provided by the invention has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20241404. The haematococcus pluvialis mutant strain obtained by screening has the characteristics of thin wall, no secondary cell wall, high astaxanthin content and the like, the haematococcus pluvialis mutant strain is high in growth speed and can quickly reach a plateau phase, the individual proportion of parental cells in a population bred by the haematococcus pluvialis mutant strain serving as a parent is higher, the cost required by extraction of natural astaxanthin can be remarkably reduced, and the haematococcus pluvialis mutant strain is suitable for industrial production. The method is suitable for large-scale popularization.
Owner:NINGBO UNIV

Device and method for extracting astaxanthin

The embodiment of the specification provides an astaxanthin extraction device, the device comprises a mixing tank, a cavitator, a static mixer, a three-phase centrifuge and a precision filter, the mixing tank is configured to mix haematococcus pluvialis, water and phospholipase to form uniform algae slurry; the cavitator is configured to receive the algae slurry and perform wall breaking on the algae slurry by utilizing hydrodynamic cavitation to obtain wall-broken algae slurry; the static mixer is configured to mix the wall-broken algae slurry and an extraction agent to form mixed algae slurry; the three-phase centrifugal machine is configured to receive the mixed algae slurry and perform three-phase separation on the mixed algae slurry to obtain an astaxanthin oil phase, a water phase and algae residues; the precision filter is configured to filter the astaxanthin oil phase so as to remove residual algae residues, and the astaxanthin oleoresin is obtained.
Owner:INNOBIO CORP LTD

Haematococcus culture solution dissolved oxygen intelligent cooperative regulation and control method and system

The invention discloses an intelligent cooperative regulation and control method and system for dissolved oxygen in a haematococcus culture solution, relates to the field of bioengineering, and controls a decision-making layer to dynamically switch between a growth promotion regulation and control mode and a stress induction regulation and control mode based on biomass data and physiological status data in a haematococcus culture stage so as to realize cooperative regulation and control of dissolved oxygen, illumination, pH and a carbon source. According to the method, a growth promotion mode and a stress induction mode are dynamically switched, biomass accumulation is promoted through dissolved oxygen sine wave pulse fluctuation in the vegetative period, astaxanthin synthesis is induced through high-oxygen and low-oxygen circulation and blue light regulation in the stress period, and compared with traditional static regulation, the biomass is increased, and the yield of astaxanthin is increased. A light-oxygen synergistic factor k is introduced to dynamically adjust the fluctuation range of dissolved oxygen, the problem of illumination-dissolved oxygen mismatching is solved, and the photosynthetic efficiency is improved; a stress intensity index SI is established, stress parameters are adaptively optimized based on the astaxanthin synthesis rate, cell damage caused by over-stress is avoided, and the stability of the astaxanthin synthesis rate in the stress period is improved.
Owner:ERFA BIOTECHNOLOGY (JIAXING) CO LTD

Green directional extraction and purification method for haematococcus astaxanthin

The invention belongs to the technical field of organic matter extraction, and particularly relates to a green directional extraction and purification method for haematococcus astaxanthin, which comprises the following steps: (1) mixing dried haematococcus powder and a composite pretreatment agent according to a mass ratio of 1: (8-15), and stirring to obtain a pretreated algae solution; (2) treating the pretreated algae liquid through ultrahigh-pressure microjet equipment to obtain wall-broken slurry; (3) mixing the wall-broken slurry and a composite extraction agent according to a volume ratio of 1: 3-1: 5, and performing extraction separation to obtain an astaxanthin extraction phase; (4) carrying out selective adsorption on the astaxanthin extraction phase through an adsorption column filled with a molecular imprinting adsorbent; (5) collecting eluent by adopting a gradient elution method; and (6) performing vacuum concentration on the eluent, and performing spray drying to obtain an astaxanthin product. According to the method, the astaxanthin extraction efficiency and the product purity are remarkably improved, meanwhile, the energy consumption is reduced to the maximum extent, toxic solvent residues are avoided, and the stability of the astaxanthin is effectively protected.
Owner:ERFA BIOTECHNOLOGY (JIAXING) CO LTD

Preparation method of astaxanthin oil

The invention relates to the technical field of astaxanthin oil, in particular to a preparation method of astaxanthin oil, which comprises the following specific steps: mixing haematococcus powder with double-particle-size glass beads, adding coconut oil, and oscillating; then cellulase is added for a hydrolysis reaction, enzyme is inactivated after hydrolysis, and a wall-broken mixture is obtained; carrying out primary extraction and secondary extraction on the wall-broken mixture, combining the two extracting solutions, and filtering; performing supercritical CO2 extraction on the filtrate to obtain primary astaxanthin oil; mixing the primary astaxanthin oil with a 1-butyl-3-methylimidazole hydroxide ethanol solution, and introducing nitrogen to carry out a reaction; cooling and filtering after the reaction is finished, and finally desolventizing under reduced pressure to obtain the astaxanthin oil. In the design, the 1-butyl-3-methylimidazole hydroxide can efficiently hydrolyze lipid impurities in the astaxanthin oil to generate water-soluble fatty acid salt and glycerol, so that the separation efficiency is improved, and the separation effect is further optimized.
Owner:云南爱尔发生物技术股份有限公司

Eye-protecting nutrition powder and preparation method thereof

The invention belongs to the technical field of health care, and particularly relates to eye protection nutrition powder and a preparation method thereof, the eye protection nutrition powder comprises glucose, whole milk powder, fruit powder, marigold powder, flaxseed powder, seaweed meal, haematococcus pluvialis powder, lutein ester, zeaxanthin, beta-carotene, ceramide and DHA algae oil powder. The eye protection nutrition powder is good in absorption effect, improves visual fatigue and yellow bottom spot lesion, and effectively protects eyes; and the concentration and memory can be improved. According to the eye-protecting nutrition powder prepared by adopting a biological wall-breaking and multi-flora fermentation technology, effective components can be dissolved out to the maximum extent after the eye-protecting nutrition powder is taken, and the nutrition powder prepared by the method is easy to absorb by a human body and high in bioavailability.
Owner:XIAMEN BENENG HEALTH IND CO LTD

Sow feed added with puffed rice and preparation method thereof

The invention provides a sow feed added with puffed rice and a preparation method of the sow feed. The sow feed comprises the following components in parts by weight: 30-50 parts of puffed rice, 10-20 parts of soybean meal, 10-20 parts of wheat bran, 2-6 parts of sugar / beet pulp polypeptide, 20-30 parts of mixed forage powder, 2-6 parts of pea fibers, 2-6 parts of pennisetum purpureum dietary fibers, 5-15 parts of manioc waste, 4-10 parts of a haematococcus extract, 2-8 parts of a cercis chinensis extract, 1-4 parts of a composite strain, 0.1-0.6 part of temperature-resistant phytase and 0.5-0.9 part of myristic acid. According to the present invention, the production method is simple, and the prepared sow feed can improve the lactation yield of sows, increase the number of immune globulins in milk, improve the immunity of piglets, improve the intestinal microbiological structure and the intestinal morphology of the piglets, reduce the diarrhea of the piglets, improve the immunity of the piglets, improve the immunity of the piglets, improve the immunity of the piglets, and improve the immunity of the piglets. The growth and development of piglets are promoted.
Owner:ZHUMADIAN HONGZHAN FEED CO LTD

Astaxanthin oil extracted from Haematococcus pluvialis and microcapsule product thereof

Recipe for a double microencapsulated powder, characterized in that the recipe comprises the following components in parts by weight: 5-60 parts by weight of core material of group A, 5-40 parts by weight of core material of group B, 5-16 parts by weight of protein shell material, 3-19 parts by weight of polysaccharide shell material, 0.1-2 parts by weight of immobilizing enzyme, 1-10 parts by weight of filler, 1-4 parts by weight of pH regulator and 0-2 parts by weight of antioxidant.
Owner:INNOBIO CORP LTD

Haematococcus pluvialis exopolysaccharide as well as preparation method and application thereof

The invention discloses a haematococcus pluvialis exopolysaccharide and a preparation method and application thereof.The preparation method of the haematococcus pluvialis exopolysaccharide comprises the step that the exopolysaccharide in haematococcus pluvialis liquid which is cultured for 30 days or above in low light is extracted through an alcohol precipitation method, specifically, the haematococcus pluvialis liquid which is cultured for 30 days or above in low light is collected, and the haematococcus pluvialis exopolysaccharide is obtained. Carrying out centrifugal treatment on the algae liquid, and collecting supernate; adding absolute ethyl alcohol into the collected supernate to enable the final concentration of the ethyl alcohol to reach 70%-80%, standing in a refrigerator at the temperature of 4 DEG C overnight, centrifuging the supernate containing the ethyl alcohol, and collecting precipitate; adding 200mL of purified water into the precipitate, and then carrying out auxiliary dissolution under an ultrasonic condition; and finally, centrifuging again, collecting a faint yellow extracellular polysaccharide solution, and freeze-drying to obtain the haematococcus pluvialis extracellular polysaccharide dry powder. According to the haematococcus pluvialis exopolysaccharide prepared by the method disclosed by the invention, the accumulation of astaxanthin in haematococcus pluvialis cells can be remarkably accelerated, the yield of astaxanthin produced by large-scale commercial culture of haematococcus pluvialis is favorably improved, and the economic benefit is obviously improved.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Method for sequentially separating polysaccharide and phycoerythrin from haematococcus degreased algae residue water extract

The invention belongs to the technical field of haematococcus separation, and particularly relates to a method for sequentially separating polysaccharide and phycoerythrin from a haematococcus degreased algae residue water extract, and the method comprises the following steps: S1, pretreatment, S2, deep flocculation and primary separation of polysaccharide, and S3, specific precipitation of phycoerythrin. According to the method, a set of sequential separation process is constructed by adopting a self-made hydrophobic flocculant and a composite precipitation inducer, so that a high-purity polysaccharide product and a high-activity phycoerythrin concentrated solution can be synchronously obtained from a haematococcus degreased algae residue water extract. According to the method, deep flocculation of polysaccharide, specific precipitation of phycoerythrin and respective subsequent refining steps are integrated into an ordered whole process, so that the product in the previous step can be directly treated in the next step in an optimal state, and the loss possibly caused by storage and transfer of intermediate materials is reduced; through condition matching and cooperation among the steps, the purity of the final product is improved.
Owner:ERFA BIOTECHNOLOGY (JIAXING) CO LTD

Novel wall breaking device for haematococcus pluvialis

The invention relates to the technical field of haematococcus cell disruption, in particular to a novel haematococcus pluvialis cell disruption device which comprises two first cell disruption barrels, a second cell disruption barrel is arranged below the middle of the two first cell disruption barrels, and a rolling assembly used for secondary cell disruption of haematococcus is arranged above the middle of the second cell disruption barrel. The rolling assembly comprises a shaft rod, a movable sleeve connected with the top end of the shaft rod in a sliding mode and four linkage structures annularly and regularly arranged on the side wall of the movable sleeve. According to the device, the first wall breaking barrel, the second wall breaking barrel and the rolling assembly are arranged to conduct secondary wall breaking on haematococcus, and the wall breaking effect is improved; meanwhile, the device is provided with a rolling assembly, so that haematococcus after primary wall breaking enters a second wall breaking barrel in batches for wall breaking, and the situation that the haematococcus after single-time wall breaking enters too much to block a filter barrel, and consequently wall breaking cannot be achieved is avoided; in addition, by arranging a vibration structure, the filtering barrel is knocked while secondary wall breaking is conducted, and the situation that haematococcus pluvialis blocks the filtering barrel, and the wall breaking filtering efficiency is affected is avoided.
Owner:YUNNAN AIERKANG BIOTECH

A method for multi-product cascade separation of haematococcus based on coupling of low eutectic solvent sequential extraction and enzyme membrane reactor

ActiveCN121371677BEffective dissociationImprove extraction efficiencyDepsipeptidesNatural dyesEnzyme membraneHaematococcus
The application belongs to the technical field of natural substance separation and extraction, and particularly relates to a kind of Haematococcus pluvialis multi-product cascade separation method based on eutectic solvent sequential extraction and enzyme membrane reactor coupling, comprising the following steps: (1) cell wall weakening pretreatment step; (2) water-soluble component extraction step; (3) pigment component extraction step; (4) astaxanthin extraction step; (5) enzyme conversion step. Through the synergistic effect of eutectic solvent sequential extraction and enzyme membrane reactor, efficient, green and continuous cascade separation of water-soluble components, pigments, astaxanthin and xylo-oligosaccharides in Haematococcus pluvialis is realized, and product purity and resource utilization are improved.
Owner:ERFA BIOTECHNOLOGY (JIAXING) CO LTD

Method for preparing tropical fish feed by using brine shrimp, and tropical fish feed prepared thereby

The present invention relates to a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, and, more specifically, to: a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, the method feeding spirulina and Haematococcus to hatched brine shrimp to prepare a tropical fish feed, and thus can promote the growth of tropical fish, boost the immune system and improve the consumption, digestion, absorption and palatability for tropical fish.
Owner:SIM HONG SUN

A synthetic astaxanthin-producing Yersinia lipophila strain, its construction method, and its application.

PendingCN122326644AReticulum cellAstaxanthin
This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.
Owner:MAIYUAN LABORATORY

Three-loop full-automatic CIP cleaning system of haematococcus pluvialis liquid treatment system

PendingCN119634379AAvoid Mixed ContaminationHollow article cleaningControl systemHaematococcus
The invention discloses a haematococcus pluvialis liquid treatment system three-loop full-automatic CIP cleaning system which comprises a cleaning unit A, a cleaning unit B, a cleaning unit C, a cleaning unit D and a three-loop full-automatic CIP system, the cleaning unit A is in butt joint with the cleaning unit B through a management system, the cleaning unit B is in butt joint with the cleaning unit D through a pipeline system, and the cleaning unit C is in butt joint with the cleaning unit D through a pipeline system. The three-loop full-automatic CIP system is in butt joint with the cleaning unit A, the cleaning unit B and the cleaning unit C through the pipeline system. According to the three-loop full-automatic CIP cleaning system of the haematococcus pluvialis liquid treatment system, the parallel design of three CIP loop systems is adopted, and the design and use of various sensors and a PLC control system are matched, so that water flow can be autonomously classified according to the real-time state of CIP backflow; and the problem that the CIP system needs to increase energy consumption adjustment and the next cleaning circulation time to cause mixed pollution is avoided.
Owner:PINGBIAN BOXIN BIOTECHNOLOGY CO LTD

Haematococcus cultivation method based on flexible LED array and photobioreactor

The invention discloses a haematococcus culture method based on a flexible LED array and a photobioreactor, and relates to the field of bioengineering, and the method comprises the following steps: preparing an SE culture medium according to the growth requirements of haematococcus; pumping the SE culture medium into the sterilized and cooled photobioreactor to a working volume through a sterile pipeline; the photobioreactor is started, sterile air with set flow is introduced, and mild stirring is carried out. According to the invention, the flexible LED array is adopted to surround the transparent reaction inner cavity, so that three-dimensional light distribution is realized, illumination dead angles are eliminated, and the light receiving uniformity of cells is greatly improved; an OD680-target light intensity linear curve is fitted through a pre-experiment, a reactor controller and an LED driving module are combined, light intensity automatic linear adjustment is achieved, light damage is avoided, and the photosynthetic efficiency is greatly improved compared with a traditional rigid LED. The photobioreactor realizes integrated control of illumination and ventilation, reduces manual operation, reduces pollution risk, is stable in product content among batches, and meets the requirements of industrial large-scale production.
Owner:云南爱尔发生物技术股份有限公司

Actate-resistant domesticated algal strain HpAc50 and application thereof

The invention discloses an acetate-resistant domesticated algal strain HpAc50 and an application thereof. The algal strain is preserved in the China Center for Type Culture Collection on January 12, 2026, and the preservation number is CCTCC M 202690. The haematococcus pluvialis strain is obtained by carrying out acetate-resistant domestication on haematococcus pluvialis and can be cultured under the condition that the acetic acid concentration is 0-10g / L, and the yield of astaxanthin is effectively increased. The invention specifically provides three application conditions: illumination of 0-5000lux and a full-nutrient culture medium; illumination is performed at 0-5000 lux, a nitrogen-phosphorus-deficient culture medium is adopted, and an exogenous stimulant is added According to the present invention, with the application of the strain, the cell density, the biomass and the astaxanthin accumulation can be significantly improved under the conditions of illumination of 5000-15000 lux and nitrogen and phosphorus deficiency culture medium, such that the strain has strong acetate tolerance and strong production performance compared with the wild type strain, and is suitable for the large-scale astaxanthin production.
Owner:ANHUI POLYTECHNIC UNIV

Method for removing hexavalent chromium ions in water body

The invention provides a method for removing hexavalent chromium ions in a water body, and relates to the technical field of treatment of wastewater containing heavy metal ions, and the method comprises the following steps: providing haematococcus pluvialis, activating the haematococcus pluvialis, and separating and purifying the haematococcus pluvialis; the method comprises the following steps: providing a culture medium and a hexavalent chromium ion-containing water body, adding the hexavalent chromium ion-containing water body into the culture medium to prepare the hexavalent chromium ion-containing culture medium, and culturing the purified haematococcus pluvialis by using the hexavalent chromium ion-containing culture medium. The purified haematococcus pluvialis grows on a culture medium containing hexavalent chromium ions, astaxanthin and beta carotene with high content are generated in cells, the cells have high reducing capacity, and high-valence Cr (VI) is reduced into Cr (III), so that the content of Cr (VI) in a water body is reduced, and the toxicity of high-valence chromium ions to algae cells is reduced.
Owner:HEZE UNIV

Method of culturing Haematococcus species for manufacturing of astaxanthin

A method of culturing Haematococcus species for manufacturing of astaxanthin comprising the steps of: providing a substrate, arranging the Haematococcus species on the surface of the substrate, exposing the Haematococcus species arranged on the substrate to high light intensities from the beginning of a culturing process and avoiding a two-step culturing process of the Haematococcus species with a first step which is an initial culturing taking place by exposure of the Haematococcus species to low light energy followed by a second step of subsequent culturing of the Haematococcus species by exposure of the Haematococcus species to higher light energy than applied in the first step to induce astaxanthin formation, and optionally—harvesting the cultured Haematococcus species and / or—isolating astaxanthin.
Owner:AD ASTRA EHF +1

A photosensitive high-astaxanthin-yielding Haematococcus pluvialis and its application

The embodiment of the present invention discloses a Haematococcus pluvialis LT4012, which has a collection number of GDMCC No. 65798 in the Guangdong Provincial Microbial Culture Collection Center. The Haematococcus pluvialis LT4012 can be used for any of the following applications: (A1) to increase the yield of astaxanthin; (A2) to prepare a product for producing astaxanthin; (A3) to produce astaxanthin under weak light; (A4) to prepare a product for inducing astaxanthin gene expression. The light-sensitive, high-yield astaxanthin Haematococcus pluvialis of the present invention has stable performance and is sensitive to strong and weak light signals. Compared with the original algae strain, the algae cells have strong stress resistance, significantly improved astaxanthin production rate, and stable yield.
Owner:BEIJING UNION UNIVERSITY

A Haematococcus pluvialis-MBR coupled biogas slurry purification device and method

The present invention belongs to the technical field of Haematococcus pluvialis cultivation, and discloses a Haematococcus pluvialis-MBR coupled biogas slurry purification device and method. The device includes a biogas slurry water inlet barrel, an MBR membrane treatment device, a water storage device and a Haematococcus pluvialis cultivation device. The present invention uses organic matter and nutrients such as nitrogen, phosphorus, potassium, and trace elements in the biogas slurry as essential elements for the growth of Haematococcus pluvialis, reduces the addition of exogenous nutrients in the cultivation of Haematococcus pluvialis, reduces the cultivation cost, and Haematococcus pluvialis can deeply treat wastewater to achieve the purpose of purification treatment, while producing a high-added product astaxanthin, thereby improving economic benefits. Haematococcus pluvialis uses CO2 to photosynthesize in the water body to release O2, reducing external oxygen supply, thereby reducing the consumption of electrical energy. At the same time, by virtue of the characteristics of Haematococcus pluvialis's fast photosynthetic rate and the accumulation of biomass by fixing CO2, CO2 emissions are reduced and the greenhouse effect is reduced.
Owner:BIOGAS SCI RES INST MIN OF AGRI

Haematococcus cultivation dissolved oxygen coupling regulation method for corrosion-resistant photoreactor

The application discloses a haematococcus cultivation dissolved oxygen coupling regulation method for a corrosion-resistant photoreactor, relates to the field of bioengineering, and builds a photoreactor and a matching regulation system made of a corrosion-resistant material; a special improved BG-11 culture medium for haematococcus is prepared and is injected into the photoreactor; a dynamic method is used to calibrate the oxygen transfer coefficient under different aeration rates, a ternary equation of light intensity, cell density and oxygen production rate is fitted in combination with the photosynthetic characteristics of haematococcus, and a dissolved oxygen dynamic prediction basic model is established. The photoreactor and the matching regulation system made of the corrosion-resistant material are built, the interference of material dissolution on the culture medium is effectively avoided, and the stability of the haematococcus cultivation environment is ensured. The oxygen transfer coefficient calibrated based on the dynamic method is combined with the ternary fitting equation of light intensity, cell density and oxygen production rate, a dissolved oxygen dynamic prediction basic model is constructed, and model prediction control and reinforcement learning intelligent agents are combined, so that precise coupling regulation of dissolved oxygen is realized.
Owner:ERFA BIOTECHNOLOGY (JIAXING) CO LTD

Improved culture solution for culturing haematococcus pluvialis and preparation method thereof

The invention belongs to the technical field of microalgae culture, and particularly provides an improved culture solution for haematococcus pluvialis culture and a preparation method thereof. The culture solution is prepared from 1.5 to 2.5 mg / L of alpha-cyclodextrin embedded sodium acetate, 0.5 to 0.5 mg / L of cobalt nitrate hexahydrate, 0.05 to 1 mg / L of copper sulfate, 0.3 to 0.9 mg / L of zinc sulfate heptahydrate, 1.5 to 2.5 mg / L of manganese chloride tetrahydrate, 3 to 7 mg / L of boric acid, 8 to 20 mg / L of sodium carbonate, 10.0 to 20.0 mg / L of disodium hydrogen phosphate dihydrate, 1200 to 1400 mg / L of sodium nitrate, 0.5 to 3 mg / L of EDTA (Ethylene Diamine Tetraacetic Acid), 20 to 50 mg / L of calcium chloride, 70 to 100 mg / L of magnesium sulfate heptahydrate, 50 to 80 mg / L of monopotassium phosphate trihydrate, 0.5 to 1.0 mg / L of ferrous sulfate heptahydrate and 0.01 to 0.03 mg / L of ammonium molybdate tetrahydrate. According to the invention, alpha-cyclodextrin is introduced to embed sodium acetate, ferrous sulfate heptahydrate and ammonium molybdate tetrahydrate are added, and a sodium carbonate-disodium hydrogen phosphate-potassium dihydrogen phosphate composite buffer system is constructed, so that the problem of easy imbalance of traditional carbon source supply is solved, and the defect of single and short elements of a traditional formula can be overcome; pH fluctuation caused by cell metabolism acid production during high-density culture can be effectively coped with, it is ensured that cells are always in a suitable acid-base environment, and nutrient absorption and metabolism pathways are stabilized.
Owner:JUKUN BIOTECHNOLOGY (YUNNAN) CO LTD

A method for efficient recovery of haematococcus cells using organic flocculation

The application belongs to the field of algal resource utilization and bioengineering technology, and specifically discloses a method for collecting Haematococcus pluvialis cells by using organic flocculation, and the specific steps are as follows: after Haematococcus pluvialis is completely converted into red spores, cassava starch is first added into the algal liquid, 80 mg of cassava starch is added into each liter of the algal liquid, uniform stirring is performed, and standing is performed for 2 hours; then, sodium alginate / chitosan composite flocculant is added into the algal liquid, 40 mg of the sodium alginate / chitosan composite flocculant is added into each liter of the algal liquid, uniform stirring is performed, standing is performed for 1 hour, the precipitate is collected, and Haematococcus pluvialis cells are obtained. The cassava starch and the sodium alginate / chitosan composite flocculant used in the application can significantly improve the flocculation rate of Haematococcus pluvialis, are non-toxic, environmentally friendly, easy to degrade, and low in cost; the method is simple to operate, difficult to apply in industrialization on a large scale, and has a wide market application prospect.
Owner:SHANGHAI OCEAN UNIV

Immobilized microbial agent with carbon sequestration and decontamination capabilities as well as preparation method and application of immobilized microbial agent

The invention provides an immobilized microbial agent with carbon sequestration and decontamination capabilities as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The immobilized microbial agent is prepared by taking bacillus glutamicum, bacillus, haematococcus, carbon sequestration ammoxidation archaea and carbon sequestration bacterium acetobacter xylinum as raw materials and taking charcoal as a carrier, and has efficient degradation capacity on dimethyl phthalate, diethyl phthalate, dibutyl phthalate, butyl benzyl phthalate and the like; and the soil carbon sequestration capability can be enhanced.
Owner:NANJING AGRICULTURAL UNIVERSITY

Directional domestication method for anti-pollution algae species of haematococcus

PendingCN121852305AEnsure that the algae species finally obtained are free of contaminationEnsure that the final algae species obtained are both economical and economicalComponent separationMicrobiological testing/measurementMicroorganismHaematococcus
The invention discloses a directional domestication method of anti-pollution algae species of haematococcus, and relates to the field of microbial breeding, key domestication indexes comprise specific growth rate, photochemical quantum yield, astaxanthin content and intracellular pollutant concentration; inputting the domestication key indexes into a resistance comprehensive scoring model, and outputting a resistance comprehensive score; and the pollutant concentration adjustment algorithm comprehensively scores according to the resistance of the current domestication period. According to the method, the resistance comprehensive scoring model is constructed, so that traditional qualitative resistance evaluation is upgraded to quantitative scoring. Scoring can objectively and comprehensively reflect the comprehensive resistance of the algal population, the problem of bifamily algal species caused by single index screening is avoided, and it is ensured that the finally obtained algal species have the anti-pollution capacity and the economic value at the same time. Whether the resistance comprehensive score reaches a target value or not is taken as a feedback signal, the pollutant concentration of the next period is automatically adjusted, the pressure is properly increased when the algal population is excellent in performance, and the pressure is timely reduced when the algal population is poor in performance. Compared with a traditional fixed gradient mode, the dynamic regulation and control mode can shorten the domestication period.
Owner:云南爱尔发生物技术股份有限公司

A rainfall runoff collection and response system for an open pond of haematococcus

This invention relates to the field of Haematococcus pluvialis cultivation technology, specifically to a rainwater runoff collection and management system for an open Haematococcus pluvialis pond. The system includes a canopy, and several water collection mechanisms arranged side-by-side on both sides of the open pond. Each water collection mechanism includes a water collection pipe located at the bottom of the sloping surface of the canopy, a diversion pipe connected in the middle of the water collection pipe, and a water storage tank connected at the bottom of the diversion pipe. This invention achieves a normally closed seal of the water collection pipe by using an arc-shaped drain rod driven by a float to block the water collection trough, ensuring the quality of collected rainwater from the source. By using a diversion ball valve with a three-way diversion channel driven by a servo motor, combined with a water quality monitoring unit and a control unit, real-time judgment and diversion of water quality are achieved. This results in the automatic discharge of heavily polluted initial rainwater and the collection of only high-quality mid-to-late-stage rainwater, significantly improving the quality of recycled water resources.
Owner:云南爱尔发生物技术股份有限公司

Sunscreen liquid foundation and preparation method thereof

The invention provides a sunscreen liquid foundation and a preparation method thereof. The sunscreen liquid foundation is prepared from the following components in parts by weight: 2 parts of a filling powder vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane cross-linked polymer, 1 part of a thickening agent, 1 part of a thickening agent, 1 part of a thickening agent and 1 part of a thickening agent; based on 2 parts of the filling powder vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane cross-linked polymer as a reference, 0.2 to 1 part of physical sunscreen powder; 2-6 parts of a chemical sunscreen composition; 5 to 15 parts of a biological sunscreen composition; the biological sunscreen composition comprises an extract obtained by mixed fermentation of cynanchum atratum, leontopodium alpinum and haematococcus pluvialis. And 22-47 parts of other components. The sunscreen liquid foundation has a sunscreen effect, is easy to smear and is mild to skin.
Owner:A & H INT COSMETICS CO LTD

A Haematococcus algae cultivation device based on the niche principle

The present invention relates to the technical field of Haematococcus algae cultivation, specifically a Haematococcus algae cultivation device based on the principle of ecological niche, including a liquid storage tank for storing Haematococcus algae solution, a main channel pipe is connected to the middle of the top surface of the liquid storage tank, a liquid pipe is suspended on the central axis of the main channel pipe, a water pump for pumping Haematococcus algae solution into the liquid pipe is installed on the top of the liquid storage tank, a plurality of culture groups are arranged on the outside of the main channel pipe, and a flipping group for controlling the rotation of a plurality of liquid introduction pipes is arranged inside the main channel pipe. The present invention pumps the prepared Haematococcus algae solution in the liquid storage tank into a plurality of culture groups through a water pump, forms ecological niches at different positions, and starts a servo motor to work at a low speed, and drives the main rotary cylinder to drive a plurality of liquid introduction pipes to rotate synchronously in different directions, so that the Haematococcus algae solution in the culture cylinder does not stand still or hang on the wall, thereby maximizing the use of sunlight and space resources to fully receive light growth.
Owner:云南爱尔发生物技术股份有限公司