217 results about "Haematococcus" patented technology

The process of cultivating haemtococcus pluvialis and producing astaxanthin includes compounding culture medium, one-step production process, circular use of the culture meidum and utilziing CO2 in regualting the pH value of culture liquid, inducing sporification of haemtococcus pluvialis and accumulating astaxanthin. The said process including cell growth of haemtococcus pulvalis, spore conversion and astaxanthin accumulation is completed in the same culture medium inside one identical bioreactor. The culture period may be shortened to 12-15 days, and the culture medium may be reused for at least six times via proper treatment. The production process has no effluent drainage, high astaxanthin yield and quality.

There is provided a method of separating free-astaxanthin selectively from green algae and, more particularly, to a method of separating free-astaxanthin selectively from Haematococcus pluvialis, the method comprising: mixing a cell culture containing Haematococcus pluvialis with an alkanic solvent and stirring, thereby obtaining an alkanic solvent extract containing astaxanthin material including free-astaxanthin and astaxanthin ester (step 1); and mixing the alkanic solvent extract with an alcohol and stirring, thereby obtaining an alcoholic extract containing free-astaxanthin (step 2). The present invention can not only maximize the efficiency of separating astaxanthin in high concentration by providing a very simple process technique capable of recovering free-astaxanthin selectively from H. pluvialis culture with high efficiency, but also realize the economical and simple operation of the process of separating free-astaxanthin by repeatedly using an alkanic solvent as a mediating solvent for extracting free-astaxanthin. Such a separation method can be used in extracting and separating a carotinoid analogue containing astaxanthin from a useful microalgae culture in addition to Haematococcus species.

The invention discloses a composition containing a haematococcus extract and a collagen peptide. The composition is prepared from the following components in part by weight: 1 to 20 parts of haematococcus pluvialis extract, 1 to 10 parts of collagen peptide and 1 to 90 parts of safflower seed oil. The invention also discloses a preparation method for the composition. Natural astaxanthin in the haematococcus pluvialis extract has the ultrahigh oxidation resistance, ensures the normal metabolism of skin, inhibits the generation of melanin and enhances the effect of collagen; the absorption rate of the collagen peptide is between 95 and 100 percent; the collagen peptide can improve the metabolism of skin cells effectively, and can be utilized by the skin cells more easily, so that the activity of the collagen in the skin is enhanced, and the aims of preserving moisture, resisting wrinkles and nourishing the skin are fulfilled; and due to the addition of the safflower seed oil, the composition contains linoleic acid with high content and vitamin E and can prevent the oxidative deterioration of the astaxanthin, an antioxidant is not needed to be added, and the quality guarantee period can be prolonged by one time.

The invention provides a haematococcus extract preparation, and a preparation method and use thereof. A haematococcus extract soft capsule provided by the invention comprises the contents of haematococcus extract and an oleic phase, wherein the astaxanthin content of the haematococcus extract is 2 to 20 percent (w/w); and the mass-to-volume ratio (g/mL) of astaxanthin in the haematococcus extract and the oleic phase is 1-10:100. The invention also provides the method for preparing the haematococcus extract soft capsules. Experiments show that the haematococcus extract soft capsule provided by the invention has stability remarkably superior to that of the haematococcus extract and conventional products and is of great importance to the application of the astaxanthin to antioxidation, the enhancement of immunity, the suppression of tumorigenesis, the prevention and treatment of cardiovascular disease, the prevention of skin aging and the maintenance of health of eyes and the central nervous system.

The invention relates to a method for quickly extracting astaxanthin from haematococcus pluvialis. The method includes particular technologies of adding organic solvents into haematococcus pluvialis powder, mixing the organic solvents with the haematococcus pluvialis powder to obtain suspension liquid, feeding the suspension liquid into explosion-proof type high-pressure homogenizers and carrying out wall breaking-extraction on the suspension liquid; separating wall-broken haematococcus pluvialis powder from the solvents to obtain astaxanthin extraction liquid and haematococcus pluvialis residues; concentrating the astaxanthin extraction liquid under the protection of inert gas and removing solvent residues to obtain the astaxanthin. The method has the advantages that the effective content yield can reach 90% at least, technological processes are short, operation is simple and speedy, and industrial production can be facilitated.

The invention belongs to the technical field of biology, relates to poultry breeding and particularly relates to nutrient enhanced laying hen feed and application thereof. The nutrient enhanced mixed feed is prepared by taking common compound chicken feed as a foundation, and adding a nutrient enhancer containing rich astaxanthin, wherein the adding amount of the nutrient enhancer containing the rich astaxanthin occupies 0.1%-5.0% of the total mass of the nutrient enhanced laying hen feed. Meanwhile, the feed is used for feeding hens during an egg laying period. The feed sufficiently utilizes the property that haematococcus contains rich natural astaxanthin, and astaxanthin has strong tinting strength and a nutritional health function; a transformation ability of laying hens is effectively used for transferring the natural astaxanthin of the haematococcus and storing the natural astaxanthin into yolk, so that nutrient enhanced functional products, which can be easily and widely accepted by the public, are produced, and requirements on dietary nutrition and health of the public are met.

The invention relates to the technical field of health food, in particular to food composition containing tissue culture of saussurea involucrate and cubilose. The food composition is prepared from 7 to 8 parts of tissue culture of saussurea involucrate, 50 parts of cubilose, 1 part of haematococcus pluvialis and 0.5 part of donkey-hide gelatin peptide, and the diameter of the tissue culture of saussurea involucrate is 20 to 100 nm. The invention provides a new type of food containing tissue culture of saussurea involucrate and cubilose, and the two kinds of raw materials which are healthy are collected in a formula, the material compositions of the two are complementary, the tissue culture of saussurea involucrate is rich in total flavonoids, total polyphenols, lilacin, chlorogenic acid and other substances, and cubilose is rich in protein, which are complementary to each other to improve the body immunity, especially for women to nourish the blood and beautifying, delay senility, and relieve dysmenorrhea.

The invention discloses an antioxidant solid drink containing haematococcus pluvialis and blackcurrant powder. The antioxidant solid drink comprises the following components in parts by weight: 0.1-6.0 parts of haematococcus pluvialis, 3-10 parts of blackcurrant juice powder, 3-10 parts of cranberry juice powder, and 3-10 parts of blueberry juice powder. Through the synergistic effect and mutual promotion of antioxidant components, the antioxidant effect of the solid drink is improved, the cost of raw materials is greatly reduced, and meanwhile, the antioxidant solid drink has the functions of promoting appetite, maintaining normal activity of nerve, protecting visual function and the like.

The invention relates to a cubilose and collagen peptide beverage. The cubilose and collagen peptide beverage is characterized by being prepared from the raw materials in percentage by weight: 8-18% of fish collagen peptides, 0.1-0.6% of cubilose powder, 1-3% of a blueberry concentrated solution, 2-4% of a passion flower concentrated solution, 1-3% of an apple concentrated solution, 0.05-0.3% of inulin, 0.1-1% of tremella polysaccharide, 0.1-0.3% of cherry powder, 0.1-0.8% of red grape concentrated fruit powder, 0.1-0.3% of stachyose, 0.5-2% of elastin peptides, 0.1-0.6% of gamma-aminobutyricacid, 0.1-1% of haematococcus pluvialis, 0.03-0.07% of a food additive, 0.01-0.03% of an essence for food, and the balance of pure water. The invention further discloses a preparation method of the cubilose and collagen peptide beverage. Two major functional components, namely cubilose and collagen peptides, of the cubilose and collagen peptide beverage disclosed by the invention complement each other, the curative effect can be exerted better, the nutrition of the cubilose and collagen peptide beverage is rich, the translation speed of a taker can be effectively increased, memory of the takercan be effectively improved, reactivation of cells and generation of collagen are promoted, nourishing before delivery, after delivery and after illnesses is facilitated, and nutrients daily essential to human bodies are supplemented.

The invention belongs to the technical field of microalgae microorganisms and relates to a wall-breaking method for haematococcus pluvialis cells which are rich in a biological active substance: astaxanthin. The wall-breaking method for the haematococcus pluvialis cells provided by the invention comprises the following three steps: 1, freezing and ice-crystallizing the haematococcus pluvialis cells and dispersing the cells in a flowing liquid below 0 DEG C; 2, adding a bioactive component which protects the haematococcus pluvialis into the liquid before the ice-crystallized algae cells are broken so as to reduce a protectant which is oxidized and decomposed after wall breaking; and 3, after uniformly grinding by a colloid mill, carrying out ultra-high-pressure wall-breaking by using an ultra-high-pressure homogeneous nanometer machine. The wall-breaking process provided by the invention is carried out under a condition of low-temperature, cold chain and close away from light and breaks walls by means of the characteristic of nanometer crushing by using the ultra-high-pressure homogeneous nanometer machine and has the advantages of rapidness, efficiency and no addition of foreign matters and can be used for effectively protecting the bioactive component.

The invention discloses a method for producing microcapsules of astaxanthin powder by utilizing haematococcus pluvialis. The method comprises the following steps: 1) breeding excellent algae species of haematococcus pluvialis; 2) obtaining cultivated species by enlarging the cultivation of algae species of haematococcus pluvialis; 3) culturing the cultivated species obtained in the step 2) by utilizing a large-scale factory microalgae culturing system so as to culture haematococcus pluvialis in a large scale; 4) stressing and inducing haematococcus pluvialis to accumulate a large amount of astaxanthin so as to obtain red spores of haematococcus pluvialis; 5) collecting the red spores of haematococcus pluvialis, which are obtained in the step 4), performing low-temperature drying so as to obtain the spore powder of haematococcus.

Belonging to the field of microalgal biotechnologies, the invention relates to a technological method for production of a microalgal energy (biodiesel) raw material from Haematococcus sp. The method includes: inoculating single-cell Haematococcus sp. into a photobioreactor, conducting swarm cell stage culture to obtain a biomass, then induce cells to transform into non-motile cells, and then performing rapid accumulation of high value-added natural astaxanthin and bio-energy grease; and finally carrying out settling and centrifugal separation to obtain high value-added astaxanthin and the microalgal energy (biodiesel) raw material. The method provided by the invention realizes production of bio-energy raw material grease at the same time of artificial induction of single-cell Haematococcus sp. in accumulation of high value-added active substances. The development mode not only improves the comprehensive utilization efficiency of microalgal biological resources, but also brings about economic feasibility for development of microalgae biodiesel.

The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell obtained from a subterranean habitat for producing the product of interest. The microalgal cell obtained from a subterranean habitat can be cultured in the dark, in light, in low nutrition, or nutrient rich conditions for at least a portion of production cycle. A combination of these conditions can be used to specifically manipulate a microalgal cell culture to produce a product of interest. The product of interest can be a water-soluble carotenoid, for example, a water-soluble carotenoid produced by culturing an algae belonging to the genus Haematococcus or a capsular exopolysaccharide produced by culturing an algae belonging to the genus Parachlorella. Compositions containing the water-soluble carotenoid, for example, as sunscreen and compositions containing the exopolysaccharide, for example, as moisturizing cream are also described.

The invention provides a haematococcus pluvialis solid dispersion, and a preparation method and applications thereof. The provided haematococcus pluvialis solid dispersion is composed of three phases namely dry algae residues prepared by subjecting haematococcus pluvialis to CO2 supercritical extraction, microencapsulated powder prepared by subjecting the haematococcus pluvialis extract obtained by CO2 supercritical extraction to microencapsulation, and a solid dispersion carrier. The invention also provides a preparation method and applications of the haematococcus pluvialis solid dispersion. The experiment results show that the astaxanthin stability, bioavailability, and anti-oxidation activity of the haematococcus pluvialis solid dispersion are obviously better than those of the cell-wall-broken haematococcus pluvialis, CO2 supercritical extract, and microencapsulated powder prepared by subjecting the CO2 supercritical extraction extract to microencapsulation.

The present invention relates to the process of regualting haemtococcus pluvialis population density and synthesizing and accumulating astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, ventilating and oxygen supplying unit, nutritious liquid compounding and temperature controlling unit and sterilizing unit; antering environment conditions to regulate haemtococcus pluvialis population density and synthesize, accumulate and collect astaxanthin; low temperature drying; etc. The natural astaxanthin is a kind of carotinoid with important physiological functions and very strong antioxidant activity.

The present invention provides a high-yielding astaxanthin mutant strain of Haematococcus pluvialis (Haematococcus pluvialis) E1 CCTCC M 207164, which uses the wild strain of Haematococcus pluvialis as material , using ultraviolet chemical compound mutagenesis, mutagenesis selection of carotenoid biosynthesis inhibitor screening. The astaxanthin content of the algal strain is 1.83 times higher than that of the original strain, the growth rate is 14% higher than that of the original strain, the physiological and biochemical characteristics are stable, and it has stable genetic properties. It is an excellent algal strain for producing natural astaxanthin and can be used as a source of industrial production. Industrial production of natural astaxanthin.

The invention discloses a method of utilizing seawater to facilitate haematococcus pluvialis to produce natural astaxanthin, and relates to the technical field of artificial cultivation of haematococcus pluvialis. The method comprises the following steps: utilizing seawater to prepare an improved culture medium of haematococcus pluvialis, adding seawater step by step to induce vegetative growth of haematococcus, cell type transformation and accumulation of astaxanthin. The method can be applied to cultivate haematococcus pluvialis at large scale at the coastal region rich in the seawater resource to produce the natural astaxanthin; compared with the conventional cultivation method of using freshwater, the method has the advantages of short production cycle, high astaxanthin content, and low material cost, and can achieve a wide industrialized application prospect.

The invention belongs to the biotechnical field, and relates to an astaxanthin wine, and a preparation method and a special wine bottle thereof. The method comprises the following steps: mixing Haematococcus pluvialis powder with ethanol, carrying out homogenization and wall breaking by using a high pressure, mixing the wall-broken Haematococcus pluvialis mud with an antioxidant, filling the wine bottle cap of the special wine bottle with the obtained mixture, storing wine in the bottle, and taking off the bottle cap during drinking to make the stored Haematococcus pluvialis mud be injected to the wine bottle and be mixed with the wine in order to obtain the astaxanthin wine. Compared with direct addition of the Haematococcus pluvialis mud into the wine and uniform mixing of the mud and the wine, the method in the invention guarantees that the astaxanthin is well rapidly dissolved in the wine, and can guarantee that the astaxanthin meets the shelf life needed by market circulation; the astaxanthin wine produced through using the method has a good appearance; and the designed wine bottle for containing the astaxanthin wine allows the Haematococcus pluvialis mud containing the astaxanthin to be injected to the wine during the cap opening, and allows the astaxanthin wine with the good appearance to be obtained through simple shaking, so a drinker does not implement extra operations, no process is increased during transportation and use, and the method is convenient, fast and effective.

The invention discloses a healthy health-preserving food formula for beautifying skins and resisting aging and a preparation method thereof, and relates to the field of healthy health-preserving foods. The formula comprises the following components in parts by weight: 2-6 parts of small molecular fish collagen, 0.005-0.015 part of fish elastin, 0.05-0.15 part of multi-petal rose powder, 0.05-0.15part of sodium hyaluronate, 0.15-0.25 part of haematococcus pluvialis, 0.1-0.25 part of yeast beta-glucan, 0.035 part of vitamin C, 0.1 part of vitamin E, 1.5-2.5 parts of freeze-dried roxburgh rose powder, and 2.5-3.5 parts of fructo-oligosaccharide. According to the invention, the homology of medicine and food is scientifically combined with new food raw materials, the traditional Chinese medicine concept is taken as the guide, both inside and outside are paid attention to, the synergistic effect and harmonious and natural beauty of the medicines are fully exerted, and the diet and the health care are organically combined so as to achieve the purpose of beautifying skins, conditioning the inside and the outside, whitening skins, removing speckles, nourishing blood, moistening skins, removing toxic substances, caring skins, delaying skin aging, resisting sunburn and resisting wrinkles.

The invention discloses a rosemary antioxidant filter capsule and an application thereof in a cigarette filter stick. A free radical scavenging agent is directly added into a cigarette and the filterstick, accordingly the stability is poor, and the activity is reduced. The rosemary antioxidant filter capsule comprises a shell and a core material, wherein the core material comprises, by weight, 2-5 parts of rosemary soluble antioxidant, 1-2 parts of a rosemary water-soluble antioxidant, 1-3 parts of haematococcus pluvialis, 2-4 parts of soluble oil and further comprises 1-2 parts of cyanobacteria and essential oil with the weight 0.2-1% of the weight of the raw materials. Compared with the prior art, the antioxidant is directly added into tobacco or a cigarette holder, and the mode that the shell wraps a capsule is used so that the oxidant can be stable and is not lost or converted after being added into the cigarette filter stick. The rosemary antioxidant, the haematococcus pluvialisand hemp seed oil are mutually permeated and fused, and have an effective adsorption effect on tar and nicotine in smoke after being mixed; suction of tar and nicotine is reduced.

The invention relates to a white fungus composite solid beverage having an anti-oxidation function and a preparation method of the white fungus composite solid beverage. The white fungus composite solid beverage is prepared from the following raw materials and components in parts by weight: 30-40 parts of white fungus ultramicro powder, 10-20 parts of fructo-oligosaccharide, 2-8 parts of inulin, 0.02-0.08 part of haematococcus pluvialis, 0.02-0.08 part of euglena, 0.02-0.08 part of a grape seed extract, 40-50 parts of sorbitol, 0.02-0.08 part of L-carnitine, and 0.02-0.08 part of vitamin C. The preparation method comprises the following steps: a step I, preparing the white fungus ultramicro powder; a step II, blending materials; a step III, performing mixing and blending; a step IV, performing granulation; and a step V, performing sterilization, performing cooling, and performing packaging. According to the white fungus composite solid beverage and the preparation method thereof disclosed by the invention, the problem of absorption of nutrients from the white funguses in the prior art is effectively solved, and the cost problem of a high-quality white fungus beverage is solved; and besides, the white fungus composite solid beverage disclosed by the invention has various efficacies of resisting oxidation, resisting ageing, enhancing immunity of organisms and the like.