66 results about "Haematococcus" patented technology

The invention discloses a plant composition with skin anti-aging activity and a preparation thereof. The plant composition is prepared from the following active ingredients in percentage by weight: 84.0 to 89.0 percent of haematococcus pluvialis extract, 8.0 to 12.0 percent of lithospermum oil, 0.5 to 0.8 percent of melaleuca alternifolia leaf oil, 0.5 to 1.0 percent of hydrolyzed olive leaf extract, 0.1 to 0.5 percent of rhodiola rosea root extract and 1.2 to 2.5 percent of bitter orange leaf oil. The plant composition with the skin anti-aging activity is used as an effective component, other auxiliaries are added, and emulsion, cream or essence oil is prepared. According to the plant composition and the preparation, the fermented camelina seed oil, the fermented safflower seed oil, the fermented meadowfoam seed oil and the like are used as solvents for extracting active components in haematococcus pluvialis and are also used as functional components and matrixes of the preparation, and compared with application of original plant oil, the plant composition and the preparation have better skin feeling and biological activity; the extraction rate of active ingredients in haematococcus pluvialis is higher, and transdermal absorption of the active ingredients is facilitated.

According to the haematococcus pluvialis strain and the application thereof, cell wall breaking operation can be carried out more easily when astaxanthin is extracted, astaxanthin can be extracted more effectively, and the cost of natural astaxanthin is reduced. The haematococcus pluvialis RMC16 strain provided by the invention has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20241404. The haematococcus pluvialis mutant strain obtained by screening has the characteristics of thin wall, no secondary cell wall, high astaxanthin content and the like, the haematococcus pluvialis mutant strain is high in growth speed and can quickly reach a plateau phase, the individual proportion of parental cells in a population bred by the haematococcus pluvialis mutant strain serving as a parent is higher, the cost required by extraction of natural astaxanthin can be remarkably reduced, and the haematococcus pluvialis mutant strain is suitable for industrial production. The method is suitable for large-scale popularization.

The embodiment of the specification provides an astaxanthin extraction device, the device comprises a mixing tank, a cavitator, a static mixer, a three-phase centrifuge and a precision filter, the mixing tank is configured to mix haematococcus pluvialis, water and phospholipase to form uniform algae slurry; the cavitator is configured to receive the algae slurry and perform wall breaking on the algae slurry by utilizing hydrodynamic cavitation to obtain wall-broken algae slurry; the static mixer is configured to mix the wall-broken algae slurry and an extraction agent to form mixed algae slurry; the three-phase centrifugal machine is configured to receive the mixed algae slurry and perform three-phase separation on the mixed algae slurry to obtain an astaxanthin oil phase, a water phase and algae residues; the precision filter is configured to filter the astaxanthin oil phase so as to remove residual algae residues, and the astaxanthin oleoresin is obtained.

The invention discloses an intelligent cooperative regulation and control method and system for dissolved oxygen in a haematococcus culture solution, relates to the field of bioengineering, and controls a decision-making layer to dynamically switch between a growth promotion regulation and control mode and a stress induction regulation and control mode based on biomass data and physiological status data in a haematococcus culture stage so as to realize cooperative regulation and control of dissolved oxygen, illumination, pH and a carbon source. According to the method, a growth promotion mode and a stress induction mode are dynamically switched, biomass accumulation is promoted through dissolved oxygen sine wave pulse fluctuation in the vegetative period, astaxanthin synthesis is induced through high-oxygen and low-oxygen circulation and blue light regulation in the stress period, and compared with traditional static regulation, the biomass is increased, and the yield of astaxanthin is increased. A light-oxygen synergistic factor k is introduced to dynamically adjust the fluctuation range of dissolved oxygen, the problem of illumination-dissolved oxygen mismatching is solved, and the photosynthetic efficiency is improved; a stress intensity index SI is established, stress parameters are adaptively optimized based on the astaxanthin synthesis rate, cell damage caused by over-stress is avoided, and the stability of the astaxanthin synthesis rate in the stress period is improved.

The invention belongs to the technical field of organic matter extraction, and particularly relates to a green directional extraction and purification method for haematococcus astaxanthin, which comprises the following steps: (1) mixing dried haematococcus powder and a composite pretreatment agent according to a mass ratio of 1: (8-15), and stirring to obtain a pretreated algae solution; (2) treating the pretreated algae liquid through ultrahigh-pressure microjet equipment to obtain wall-broken slurry; (3) mixing the wall-broken slurry and a composite extraction agent according to a volume ratio of 1: 3-1: 5, and performing extraction separation to obtain an astaxanthin extraction phase; (4) carrying out selective adsorption on the astaxanthin extraction phase through an adsorption column filled with a molecular imprinting adsorbent; (5) collecting eluent by adopting a gradient elution method; and (6) performing vacuum concentration on the eluent, and performing spray drying to obtain an astaxanthin product. According to the method, the astaxanthin extraction efficiency and the product purity are remarkably improved, meanwhile, the energy consumption is reduced to the maximum extent, toxic solvent residues are avoided, and the stability of the astaxanthin is effectively protected.

The invention relates to the technical field of astaxanthin oil, in particular to a preparation method of astaxanthin oil, which comprises the following specific steps: mixing haematococcus powder with double-particle-size glass beads, adding coconut oil, and oscillating; then cellulase is added for a hydrolysis reaction, enzyme is inactivated after hydrolysis, and a wall-broken mixture is obtained; carrying out primary extraction and secondary extraction on the wall-broken mixture, combining the two extracting solutions, and filtering; performing supercritical CO2 extraction on the filtrate to obtain primary astaxanthin oil; mixing the primary astaxanthin oil with a 1-butyl-3-methylimidazole hydroxide ethanol solution, and introducing nitrogen to carry out a reaction; cooling and filtering after the reaction is finished, and finally desolventizing under reduced pressure to obtain the astaxanthin oil. In the design, the 1-butyl-3-methylimidazole hydroxide can efficiently hydrolyze lipid impurities in the astaxanthin oil to generate water-soluble fatty acid salt and glycerol, so that the separation efficiency is improved, and the separation effect is further optimized.

Recipe for a double microencapsulated powder, characterized in that the recipe comprises the following components in parts by weight: 5-60 parts by weight of core material of group A, 5-40 parts by weight of core material of group B, 5-16 parts by weight of protein shell material, 3-19 parts by weight of polysaccharide shell material, 0.1-2 parts by weight of immobilizing enzyme, 1-10 parts by weight of filler, 1-4 parts by weight of pH regulator and 0-2 parts by weight of antioxidant.

The invention discloses a haematococcus pluvialis exopolysaccharide and a preparation method and application thereof.The preparation method of the haematococcus pluvialis exopolysaccharide comprises the step that the exopolysaccharide in haematococcus pluvialis liquid which is cultured for 30 days or above in low light is extracted through an alcohol precipitation method, specifically, the haematococcus pluvialis liquid which is cultured for 30 days or above in low light is collected, and the haematococcus pluvialis exopolysaccharide is obtained. Carrying out centrifugal treatment on the algae liquid, and collecting supernate; adding absolute ethyl alcohol into the collected supernate to enable the final concentration of the ethyl alcohol to reach 70%-80%, standing in a refrigerator at the temperature of 4 DEG C overnight, centrifuging the supernate containing the ethyl alcohol, and collecting precipitate; adding 200mL of purified water into the precipitate, and then carrying out auxiliary dissolution under an ultrasonic condition; and finally, centrifuging again, collecting a faint yellow extracellular polysaccharide solution, and freeze-drying to obtain the haematococcus pluvialis extracellular polysaccharide dry powder. According to the haematococcus pluvialis exopolysaccharide prepared by the method disclosed by the invention, the accumulation of astaxanthin in haematococcus pluvialis cells can be remarkably accelerated, the yield of astaxanthin produced by large-scale commercial culture of haematococcus pluvialis is favorably improved, and the economic benefit is obviously improved.

The invention belongs to the technical field of haematococcus separation, and particularly relates to a method for sequentially separating polysaccharide and phycoerythrin from a haematococcus degreased algae residue water extract, and the method comprises the following steps: S1, pretreatment, S2, deep flocculation and primary separation of polysaccharide, and S3, specific precipitation of phycoerythrin. According to the method, a set of sequential separation process is constructed by adopting a self-made hydrophobic flocculant and a composite precipitation inducer, so that a high-purity polysaccharide product and a high-activity phycoerythrin concentrated solution can be synchronously obtained from a haematococcus degreased algae residue water extract. According to the method, deep flocculation of polysaccharide, specific precipitation of phycoerythrin and respective subsequent refining steps are integrated into an ordered whole process, so that the product in the previous step can be directly treated in the next step in an optimal state, and the loss possibly caused by storage and transfer of intermediate materials is reduced; through condition matching and cooperation among the steps, the purity of the final product is improved.

The invention relates to the technical field of haematococcus cell disruption, in particular to a novel haematococcus pluvialis cell disruption device which comprises two first cell disruption barrels, a second cell disruption barrel is arranged below the middle of the two first cell disruption barrels, and a rolling assembly used for secondary cell disruption of haematococcus is arranged above the middle of the second cell disruption barrel. The rolling assembly comprises a shaft rod, a movable sleeve connected with the top end of the shaft rod in a sliding mode and four linkage structures annularly and regularly arranged on the side wall of the movable sleeve. According to the device, the first wall breaking barrel, the second wall breaking barrel and the rolling assembly are arranged to conduct secondary wall breaking on haematococcus, and the wall breaking effect is improved; meanwhile, the device is provided with a rolling assembly, so that haematococcus after primary wall breaking enters a second wall breaking barrel in batches for wall breaking, and the situation that the haematococcus after single-time wall breaking enters too much to block a filter barrel, and consequently wall breaking cannot be achieved is avoided; in addition, by arranging a vibration structure, the filtering barrel is knocked while secondary wall breaking is conducted, and the situation that haematococcus pluvialis blocks the filtering barrel, and the wall breaking filtering efficiency is affected is avoided.

The application belongs to the technical field of natural substance separation and extraction, and particularly relates to a kind of Haematococcus pluvialis multi-product cascade separation method based on eutectic solvent sequential extraction and enzyme membrane reactor coupling, comprising the following steps: (1) cell wall weakening pretreatment step; (2) water-soluble component extraction step; (3) pigment component extraction step; (4) astaxanthin extraction step; (5) enzyme conversion step. Through the synergistic effect of eutectic solvent sequential extraction and enzyme membrane reactor, efficient, green and continuous cascade separation of water-soluble components, pigments, astaxanthin and xylo-oligosaccharides in Haematococcus pluvialis is realized, and product purity and resource utilization are improved.

The present invention relates to a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, and, more specifically, to: a method for preparing a tropical fish feed by using brine shrimp, and a tropical fish feed prepared thereby, the method feeding spirulina and Haematococcus to hatched brine shrimp to prepare a tropical fish feed, and thus can promote the growth of tropical fish, boost the immune system and improve the consumption, digestion, absorption and palatability for tropical fish.

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

The invention discloses a haematococcus culture method based on a flexible LED array and a photobioreactor, and relates to the field of bioengineering, and the method comprises the following steps: preparing an SE culture medium according to the growth requirements of haematococcus; pumping the SE culture medium into the sterilized and cooled photobioreactor to a working volume through a sterile pipeline; the photobioreactor is started, sterile air with set flow is introduced, and mild stirring is carried out. According to the invention, the flexible LED array is adopted to surround the transparent reaction inner cavity, so that three-dimensional light distribution is realized, illumination dead angles are eliminated, and the light receiving uniformity of cells is greatly improved; an OD680-target light intensity linear curve is fitted through a pre-experiment, a reactor controller and an LED driving module are combined, light intensity automatic linear adjustment is achieved, light damage is avoided, and the photosynthetic efficiency is greatly improved compared with a traditional rigid LED. The photobioreactor realizes integrated control of illumination and ventilation, reduces manual operation, reduces pollution risk, is stable in product content among batches, and meets the requirements of industrial large-scale production.

The invention discloses an acetate-resistant domesticated algal strain HpAc50 and an application thereof. The algal strain is preserved in the China Center for Type Culture Collection on January 12, 2026, and the preservation number is CCTCC M 202690. The haematococcus pluvialis strain is obtained by carrying out acetate-resistant domestication on haematococcus pluvialis and can be cultured under the condition that the acetic acid concentration is 0-10g / L, and the yield of astaxanthin is effectively increased. The invention specifically provides three application conditions: illumination of 0-5000lux and a full-nutrient culture medium; illumination is performed at 0-5000 lux, a nitrogen-phosphorus-deficient culture medium is adopted, and an exogenous stimulant is added According to the present invention, with the application of the strain, the cell density, the biomass and the astaxanthin accumulation can be significantly improved under the conditions of illumination of 5000-15000 lux and nitrogen and phosphorus deficiency culture medium, such that the strain has strong acetate tolerance and strong production performance compared with the wild type strain, and is suitable for the large-scale astaxanthin production.

The invention provides a method for removing hexavalent chromium ions in a water body, and relates to the technical field of treatment of wastewater containing heavy metal ions, and the method comprises the following steps: providing haematococcus pluvialis, activating the haematococcus pluvialis, and separating and purifying the haematococcus pluvialis; the method comprises the following steps: providing a culture medium and a hexavalent chromium ion-containing water body, adding the hexavalent chromium ion-containing water body into the culture medium to prepare the hexavalent chromium ion-containing culture medium, and culturing the purified haematococcus pluvialis by using the hexavalent chromium ion-containing culture medium. The purified haematococcus pluvialis grows on a culture medium containing hexavalent chromium ions, astaxanthin and beta carotene with high content are generated in cells, the cells have high reducing capacity, and high-valence Cr (VI) is reduced into Cr (III), so that the content of Cr (VI) in a water body is reduced, and the toxicity of high-valence chromium ions to algae cells is reduced.

The embodiment of the present invention discloses a Haematococcus pluvialis LT4012, which has a collection number of GDMCC No. 65798 in the Guangdong Provincial Microbial Culture Collection Center. The Haematococcus pluvialis LT4012 can be used for any of the following applications: (A1) to increase the yield of astaxanthin; (A2) to prepare a product for producing astaxanthin; (A3) to produce astaxanthin under weak light; (A4) to prepare a product for inducing astaxanthin gene expression. The light-sensitive, high-yield astaxanthin Haematococcus pluvialis of the present invention has stable performance and is sensitive to strong and weak light signals. Compared with the original algae strain, the algae cells have strong stress resistance, significantly improved astaxanthin production rate, and stable yield.

The present invention belongs to the technical field of Haematococcus pluvialis cultivation, and discloses a Haematococcus pluvialis-MBR coupled biogas slurry purification device and method. The device includes a biogas slurry water inlet barrel, an MBR membrane treatment device, a water storage device and a Haematococcus pluvialis cultivation device. The present invention uses organic matter and nutrients such as nitrogen, phosphorus, potassium, and trace elements in the biogas slurry as essential elements for the growth of Haematococcus pluvialis, reduces the addition of exogenous nutrients in the cultivation of Haematococcus pluvialis, reduces the cultivation cost, and Haematococcus pluvialis can deeply treat wastewater to achieve the purpose of purification treatment, while producing a high-added product astaxanthin, thereby improving economic benefits. Haematococcus pluvialis uses CO2 to photosynthesize in the water body to release O2, reducing external oxygen supply, thereby reducing the consumption of electrical energy. At the same time, by virtue of the characteristics of Haematococcus pluvialis's fast photosynthetic rate and the accumulation of biomass by fixing CO2, CO2 emissions are reduced and the greenhouse effect is reduced.

The application discloses a haematococcus cultivation dissolved oxygen coupling regulation method for a corrosion-resistant photoreactor, relates to the field of bioengineering, and builds a photoreactor and a matching regulation system made of a corrosion-resistant material; a special improved BG-11 culture medium for haematococcus is prepared and is injected into the photoreactor; a dynamic method is used to calibrate the oxygen transfer coefficient under different aeration rates, a ternary equation of light intensity, cell density and oxygen production rate is fitted in combination with the photosynthetic characteristics of haematococcus, and a dissolved oxygen dynamic prediction basic model is established. The photoreactor and the matching regulation system made of the corrosion-resistant material are built, the interference of material dissolution on the culture medium is effectively avoided, and the stability of the haematococcus cultivation environment is ensured. The oxygen transfer coefficient calibrated based on the dynamic method is combined with the ternary fitting equation of light intensity, cell density and oxygen production rate, a dissolved oxygen dynamic prediction basic model is constructed, and model prediction control and reinforcement learning intelligent agents are combined, so that precise coupling regulation of dissolved oxygen is realized.

The invention belongs to the technical field of microalgae culture, and particularly provides an improved culture solution for haematococcus pluvialis culture and a preparation method thereof. The culture solution is prepared from 1.5 to 2.5 mg / L of alpha-cyclodextrin embedded sodium acetate, 0.5 to 0.5 mg / L of cobalt nitrate hexahydrate, 0.05 to 1 mg / L of copper sulfate, 0.3 to 0.9 mg / L of zinc sulfate heptahydrate, 1.5 to 2.5 mg / L of manganese chloride tetrahydrate, 3 to 7 mg / L of boric acid, 8 to 20 mg / L of sodium carbonate, 10.0 to 20.0 mg / L of disodium hydrogen phosphate dihydrate, 1200 to 1400 mg / L of sodium nitrate, 0.5 to 3 mg / L of EDTA (Ethylene Diamine Tetraacetic Acid), 20 to 50 mg / L of calcium chloride, 70 to 100 mg / L of magnesium sulfate heptahydrate, 50 to 80 mg / L of monopotassium phosphate trihydrate, 0.5 to 1.0 mg / L of ferrous sulfate heptahydrate and 0.01 to 0.03 mg / L of ammonium molybdate tetrahydrate. According to the invention, alpha-cyclodextrin is introduced to embed sodium acetate, ferrous sulfate heptahydrate and ammonium molybdate tetrahydrate are added, and a sodium carbonate-disodium hydrogen phosphate-potassium dihydrogen phosphate composite buffer system is constructed, so that the problem of easy imbalance of traditional carbon source supply is solved, and the defect of single and short elements of a traditional formula can be overcome; pH fluctuation caused by cell metabolism acid production during high-density culture can be effectively coped with, it is ensured that cells are always in a suitable acid-base environment, and nutrient absorption and metabolism pathways are stabilized.

The application belongs to the field of algal resource utilization and bioengineering technology, and specifically discloses a method for collecting Haematococcus pluvialis cells by using organic flocculation, and the specific steps are as follows: after Haematococcus pluvialis is completely converted into red spores, cassava starch is first added into the algal liquid, 80 mg of cassava starch is added into each liter of the algal liquid, uniform stirring is performed, and standing is performed for 2 hours; then, sodium alginate / chitosan composite flocculant is added into the algal liquid, 40 mg of the sodium alginate / chitosan composite flocculant is added into each liter of the algal liquid, uniform stirring is performed, standing is performed for 1 hour, the precipitate is collected, and Haematococcus pluvialis cells are obtained. The cassava starch and the sodium alginate / chitosan composite flocculant used in the application can significantly improve the flocculation rate of Haematococcus pluvialis, are non-toxic, environmentally friendly, easy to degrade, and low in cost; the method is simple to operate, difficult to apply in industrialization on a large scale, and has a wide market application prospect.

The invention provides an immobilized microbial agent with carbon sequestration and decontamination capabilities as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The immobilized microbial agent is prepared by taking bacillus glutamicum, bacillus, haematococcus, carbon sequestration ammoxidation archaea and carbon sequestration bacterium acetobacter xylinum as raw materials and taking charcoal as a carrier, and has efficient degradation capacity on dimethyl phthalate, diethyl phthalate, dibutyl phthalate, butyl benzyl phthalate and the like; and the soil carbon sequestration capability can be enhanced.

The invention discloses a directional domestication method of anti-pollution algae species of haematococcus, and relates to the field of microbial breeding, key domestication indexes comprise specific growth rate, photochemical quantum yield, astaxanthin content and intracellular pollutant concentration; inputting the domestication key indexes into a resistance comprehensive scoring model, and outputting a resistance comprehensive score; and the pollutant concentration adjustment algorithm comprehensively scores according to the resistance of the current domestication period. According to the method, the resistance comprehensive scoring model is constructed, so that traditional qualitative resistance evaluation is upgraded to quantitative scoring. Scoring can objectively and comprehensively reflect the comprehensive resistance of the algal population, the problem of bifamily algal species caused by single index screening is avoided, and it is ensured that the finally obtained algal species have the anti-pollution capacity and the economic value at the same time. Whether the resistance comprehensive score reaches a target value or not is taken as a feedback signal, the pollutant concentration of the next period is automatically adjusted, the pressure is properly increased when the algal population is excellent in performance, and the pressure is timely reduced when the algal population is poor in performance. Compared with a traditional fixed gradient mode, the dynamic regulation and control mode can shorten the domestication period.

This invention relates to the field of Haematococcus pluvialis cultivation technology, specifically to a rainwater runoff collection and management system for an open Haematococcus pluvialis pond. The system includes a canopy, and several water collection mechanisms arranged side-by-side on both sides of the open pond. Each water collection mechanism includes a water collection pipe located at the bottom of the sloping surface of the canopy, a diversion pipe connected in the middle of the water collection pipe, and a water storage tank connected at the bottom of the diversion pipe. This invention achieves a normally closed seal of the water collection pipe by using an arc-shaped drain rod driven by a float to block the water collection trough, ensuring the quality of collected rainwater from the source. By using a diversion ball valve with a three-way diversion channel driven by a servo motor, combined with a water quality monitoring unit and a control unit, real-time judgment and diversion of water quality are achieved. This results in the automatic discharge of heavily polluted initial rainwater and the collection of only high-quality mid-to-late-stage rainwater, significantly improving the quality of recycled water resources.

The invention discloses a high-light-resistant algal strain high-speed screening method and system based on micro-fluidic single cell sorting, and relates to the field of bioengineering.The high-light-resistant algal strain high-speed screening method comprises the steps that haematococcus free cells in the logarithmic phase are taken and added into a double-fluorescence probe mixed solution, and incubation is conducted in a dark place to obtain a haematococcus solution; bBM sheath fluid and haematococcus fluid are introduced into a sample introduction area of the micro-fluidic chip, the BBM sheath fluid and the haematococcus fluid are focused by the sample introduction area and then enter a highlight stress area, highlight treatment is carried out to induce motile cells to be transformed into spores, and the transformed cells enter a detection area. Through in-situ high light stress and single cell continuous sorting of the micro-fluidic chip, the sorting efficiency is high, and the screening period is greatly shortened; three parameters of chlorophyll fluorescence, astaxanthin fluorescence and forward scattering light are adopted for cooperative detection, and a threshold value which is quantitatively set based on a control group is combined, so that the proportion of a false positive strain is reduced to be less than 5%, and the screening accuracy of a double-superior strain is greatly improved; the microfluidic chip is extremely low in single reagent consumption, greatly reduces the screening cost of single strains, and is easy for large-scale popularization and application.

The invention relates to the technical field of haematococcus production equipment, in particular to a haematococcus fresh algae heavy metal cleaning device and a circulating cleaning process, the haematococcus fresh algae heavy metal cleaning device comprises a stirring tank and a tubular centrifuge arranged below the stirring tank, and a circulating device is further arranged below the stirring tank; the circulating device comprises a pair of mixing tanks, an adjusting part arranged on the outer wall of the mixing tank on the left side, a liquid passing part arranged between the pair of mixing tanks, a filtering part and a liquid pump. According to the haematococcus fresh algae heavy metal cleaning device and the cyclic cleaning process, through cooperative linkage of the adjusting part driven by the first electric push rod and the second electric push rod and the liquid passing part, automation of self-adaptive adjustment of the extraction height, intelligent opening and closing of a pipeline and state switching of the filtering unit is achieved; the intermittent operation depending on manual transfer, dosing and monitoring in the traditional process is replaced, and the stability and controllability of production are improved.

The invention relates to the technical field of astaxanthin measuring equipment, in particular to a method for rapidly measuring the content of astaxanthin in haematococcus, which is characterized in that a supporting bin and a driving mechanism arranged in the supporting bin are matched with a liquid separation part, and liquid in a homogenizing cup is subpackaged into a rotating part; the driving mechanism comprises an electric push rod, a supporting frame driven by the electric push rod, a protruding rod arranged on the outer wall of the supporting frame, a bending plate sliding in the supporting bin and a toothed plate moving along with the bending plate. According to the method for rapidly determining the content of astaxanthin in haematococcus, automatic switching of sub-packaging stations and continuity of sample treatment are achieved through linkage of a rotating part and a driving mechanism, a toothed plate can be meshed with ring teeth during transverse movement, a rotating shaft and a rotating disc are driven to rotate at a fixed distance through a ratchet wheel and pawl mechanism, and the content of astaxanthin in haematococcus is determined. A new centrifugal tube is moved to the position below the lower through tube, it is ensured that manual intervention is not needed for tube replacement in the liquid separation process, and high-flux continuous operation is achieved.

This invention provides a method for extracting astaxanthin from Haematococcus pluvialis and its application. The method includes the following steps: S1. Raw material pretreatment; S2. Enzymatic hydrolysis with a complex enzyme; S3. Solubilization with a surfactant; S4. Adsorption by iron(III) oxide-cellulose porous microspheres; S5. Magnetic separation and washing; S6. Astaxanthin elution and microsphere recovery; S7. Concentration and drying. This invention utilizes a complex enzyme consisting of cellulase, alginate lyase, and mannanase to synergistically hydrolyze the multilayer polysaccharide structure of thick-walled spores of Haematococcus pluvialis, thereby fully releasing astaxanthin. Furthermore, iron(III) oxide-cellulose porous microspheres with benzyl and octyl surface modifications are used for highly selective adsorption of astaxanthin, combined with magnetic separation technology, thus improving the astaxanthin yield. The extraction process of this invention is carried out under medium-low temperature conditions, and butylated hydroxytoluene is added to the eluent, effectively avoiding thermal degradation, photolysis, and oxidation of astaxanthin, resulting in a high retention rate of antioxidant activity in the obtained astaxanthin.

An object of the present invention is to provide an eye care composition which can be produced with a reduced risk of failure during production by not using a bilberry extract raw material, has good formulation stability, and can be reduced in dosage.SOLUTION: The above object can be achieved by an eye care composition comprising a Haematococcus alga extract, a marigold extract, a propolis extract and vitamin A as active ingredients, and an eye care composition comprising astaxanthin, lutein, zeaxanthin, a propolis extract and vitamin A as active ingredients.SELECTED DRAWING: None

The utility model belongs to the technical field of aquaculture equipment, and discloses an outdoor haematococcus culture glass tube quick connector which comprises connecting sleeve bodies, the connecting sleeve bodies are distributed in a mirror symmetry mode, and glass tube inserting end parts are arranged on the opposite sides of the connecting sleeve bodies in a penetrating mode. An outer inserting magnetic ring is arranged at the end, close to the connecting sleeve body on the other side, of the connecting sleeve body on one side, an inner inserting magnetic ring is arranged in the end, close to the connecting sleeve body on one side, of the connecting sleeve body on the other side, and an inserting groove is formed between the inner wall of the connecting sleeve body on the other side and the inner inserting magnetic ring. According to the connecting sleeve, instant and rapid connection of the two glass tubes is successfully achieved, the installation efficiency is greatly improved, the original connection operation which may consume tens of minutes is greatly shortened to several seconds, the building time of a breeding system is remarkably shortened, and the construction cost of the breeding system is reduced. The sealing performance creates an extremely stable and clean growth environment for haematococcus, and the pollution risk caused by leakage is reduced to the minimum.