12 results about "Haematococcus" patented technology

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

This invention relates to the field of Haematococcus pluvialis cultivation technology, specifically to a rainwater runoff collection and management system for an open Haematococcus pluvialis pond. The system includes a canopy, and several water collection mechanisms arranged side-by-side on both sides of the open pond. Each water collection mechanism includes a water collection pipe located at the bottom of the sloping surface of the canopy, a diversion pipe connected in the middle of the water collection pipe, and a water storage tank connected at the bottom of the diversion pipe. This invention achieves a normally closed seal of the water collection pipe by using an arc-shaped drain rod driven by a float to block the water collection trough, ensuring the quality of collected rainwater from the source. By using a diversion ball valve with a three-way diversion channel driven by a servo motor, combined with a water quality monitoring unit and a control unit, real-time judgment and diversion of water quality are achieved. This results in the automatic discharge of heavily polluted initial rainwater and the collection of only high-quality mid-to-late-stage rainwater, significantly improving the quality of recycled water resources.

This invention provides a method for extracting astaxanthin from Haematococcus pluvialis and its application. The method includes the following steps: S1. Raw material pretreatment; S2. Enzymatic hydrolysis with a complex enzyme; S3. Solubilization with a surfactant; S4. Adsorption by iron(III) oxide-cellulose porous microspheres; S5. Magnetic separation and washing; S6. Astaxanthin elution and microsphere recovery; S7. Concentration and drying. This invention utilizes a complex enzyme consisting of cellulase, alginate lyase, and mannanase to synergistically hydrolyze the multilayer polysaccharide structure of thick-walled spores of Haematococcus pluvialis, thereby fully releasing astaxanthin. Furthermore, iron(III) oxide-cellulose porous microspheres with benzyl and octyl surface modifications are used for highly selective adsorption of astaxanthin, combined with magnetic separation technology, thus improving the astaxanthin yield. The extraction process of this invention is carried out under medium-low temperature conditions, and butylated hydroxytoluene is added to the eluent, effectively avoiding thermal degradation, photolysis, and oxidation of astaxanthin, resulting in a high retention rate of antioxidant activity in the obtained astaxanthin.

The application belongs to the technical field of haematococcus pluvialis membrane separation, and particularly relates to a microfiltration membrane module for separating haematococcus pluvialis broken wall liquid and application thereof. The hollow fiber membrane bundle is composed of polyvinylidene fluoride hollow fiber membranes with surface grafted amphiphilic ion copolymer, the amphiphilic ion copolymer is carboxylated sulfobetaine type amphiphilic ion copolymer, the repeating unit of the copolymer contains quaternary ammonium salt positive charge group, sulfonate negative charge group and side chain carboxyl group at the same time, and the amphiphilic ion copolymer is grafted on the polyvinylidene fluoride base membrane surface through covalent bond. The microfiltration membrane module is used for separating haematococcus pluvialis broken wall liquid. The application realizes high-efficiency and stable operation without frequent chemical cleaning in the separation of haematococcus pluvialis broken wall liquid by synthesizing carboxylated sulfobetaine type amphiphilic ion copolymer and anchoring on the membrane surface through covalent bond, constructing stable anti-pollution interface with hydration layer and electrostatic double repulsion, and regulating membrane pore size with single external coagulation bath temperature.

The present application relates to the technical field of haematococcus pluvialis breaking wall, specifically, it relates to a new haematococcus pluvialis breaking wall device, including two first breaking wall barrels, a second breaking wall barrel is arranged below the middle of the two first breaking wall barrels, a rolling assembly for secondary haematococcus pluvialis breaking wall is arranged above the middle of the second breaking wall barrel, the rolling assembly includes a shaft, a movable sleeve slidingly connected with the top end of the shaft and four linkage structures regularly arranged in the form of a ring on the sidewall of the movable sleeve. The device performs secondary breaking wall on haematococcus pluvialis by setting the first breaking wall barrel, the second breaking wall barrel and the rolling assembly, and improves the breaking wall effect; meanwhile, the device makes the haematococcus pluvialis after the first breaking wall enter the second breaking wall barrel in batches to avoid too much haematococcus pluvialis entering the filter barrel at a time, which causes the breaking wall to be impossible; in addition, the vibration structure is arranged to knock the filter barrel while the secondary breaking wall is performed, which avoids haematococcus pluvialis blocking the filter barrel and affects the breaking wall filtering efficiency.

The present application belongs to the technical field of red claw crayfish breeding, and particularly relates to a fermented feed for improving the quality and immunity of red claw crayfish and a preparation method thereof. The fermented feed for improving the quality and immunity of red claw crayfish is prepared by fermenting the following raw materials in parts by weight: 15-25 parts of imported fish meal, 12-20 parts of fermented soybean meal, 5-8 parts of shrimp shell powder, 3-6 parts of spirulina powder, 5-10 parts of corn gluten meal, 10-15 parts of high-gluten flour, 3-5 parts of yeast extract, 2-4 parts of soybean phospholipid, 3-5 parts of fish oil, 1-2 parts of calcium dihydrogen phosphate, 1-2 parts of multi-vitamin and multi-mineral, 0.1-0.3 parts of beta-glucan, 2-5 parts of compound probiotic fermentation agent, and 32-40 parts of water. The compound probiotic fermentation agent is prepared by mixing Haematococcus pluvialis, Bacillus circulans and marine Rhodotorula in a mass ratio of 3-5:3-4:2-3. The fermented feed of the present application can significantly improve the body color, muscle quality and immunity of the crayfish.

This utility model relates to the field of algae cultivation, and more particularly to a mixed gas supply device for an indoor propagation workshop. The indoor propagation workshop mixed gas supply device includes an air supply unit, a carbon dioxide supply unit, a gas mixing unit, a gas filtration unit, and a gas distribution unit, all connected by pipelines. The air supply unit includes an air compressor, a first self-regulating pressure constant valve, and an air buffer storage tank. The indoor propagation workshop mixed gas supply device provided by this utility model, by installing a flow meter on the inlet pipe of the mixing device in the gas mixing unit, can precisely control the flow ratio of air to carbon dioxide to 1%-4%, ensuring stable mixed gas composition, meeting the stringent gas composition requirements of Haematococcus pluvialis and green algae during the propagation stage, improving photosynthetic efficiency, and promoting rapid cell division and expansion of Haematococcus pluvialis during the reproductive growth stage.

This invention provides a chemical mutagenesis screening method and its application for high-yield astaxanthin-producing Haematococcus pluvialis. The method involves chemically mutagenesizing Haematococcus pluvialis cells in the logarithmic growth phase with ethyl methanesulfonate, followed by single-colony screening to obtain high-yield astaxanthin mutants. After inducing astaxanthin accumulation under suitable culture conditions, the algae are freeze-dried, and then astaxanthin is extracted using a eutectic solvent system formed by thymol and oleic acid. This improves astaxanthin yield and extraction efficiency while reducing environmental pollution caused by traditional organic solvent extraction. This invention is simple to operate, has high extraction efficiency, and shows promising application prospects.

This invention belongs to the field of microbial fermentation technology, specifically relating to a highly efficient Haematococcus pluvialis mutant strain for astaxanthin production and its applications. This invention achieves efficient and stable astaxanthin production from Haematococcus pluvialis through targeted strain modification and optimized culture process. The invention obtained a highly efficient astaxanthin-producing Haematococcus pluvialis mutant strain by ARTP mutagenesis, β-ionone domestication, and glycerol domestication. Furthermore, this invention also employs lightless heterotrophic growth, short-term weak light activation, and lightless salt stress to induce astaxanthin production in the Haematococcus pluvialis mutant strain. The Haematococcus pluvialis mutant strain provided by this invention can achieve high-density biomass accumulation and high astaxanthin production in a closed fermenter, with a cell dry weight exceeding 20 g / L and an astaxanthin content reaching 11%. It also has the advantages of a short fermentation cycle and strong batch-to-batch stability, making it feasible for industrial-scale application.

This invention relates to the field of bioengineering technology, specifically to a method for promoting the growth of Haematococcus pluvialis in its green algal stage using L-Abrine. The method significantly promotes cell proliferation and biomass accumulation in the green algal stage of Haematococcus pluvialis by adding an appropriate concentration of L-Abrine to the culture medium. Experimental results show that L-Abrine significantly promotes biomass accumulation in Haematococcus pluvialis, increasing biomass by 36.36%–54.54% compared to the control group. This method is simple to operate, cost-effective, and has stable results. It requires no changes to existing culture equipment and conditions; efficient growth promotion can be achieved simply by adding L-Abrine. This invention provides a new technical approach for the large-scale cultivation of Haematococcus pluvialis and the efficient production of astaxanthin, with promising industrial application prospects, and provides sufficient material conditions for the subsequent synthesis of astaxanthin from Haematococcus pluvialis.

This disclosure relates to the field of microbial technology, and provides a Haematococcus pluvialis RXXHp1, which was deposited at the China Center for Type Culture Collection on May 19, 2023, with accession number CCTCC NO: M 2023791. This disclosure also provides an application of the above-mentioned Haematococcus pluvialis RXXHp1, including: using Haematococcus pluvialis RXXHp1 to enrich at least one of organic selenium and astaxanthin under selenium ion stress. This disclosure also provides algal powder comprising the above-mentioned Haematococcus pluvialis RXXHp1, and the application of the above-mentioned Haematococcus pluvialis RXXHp1 or the above-mentioned algal powder in the preparation of wine. The Haematococcus pluvialis RXXHp1 disclosed herein exhibits a certain tolerance to selenium ion stress, not only enriching organic selenium but also promoting the accumulation of astaxanthin. Furthermore, using the aforementioned Haematococcus pluvialis RXXHp1 in winemaking can significantly increase the content of active substances such as astaxanthin and lutein in the wine, thereby improving the color and flavor of the wine and enhancing its antioxidant capacity.

This invention relates to the field of Haematococcus pluvialis cultivation technology, specifically to a cultivation environment control device that utilizes the synergistic effect of microorganisms to promote astaxanthin synthesis in Haematococcus pluvialis. The device includes a control section installed between two adjacent cultivation tubes and a sensor probe assembly embedded in the control section. The control section includes a sampling tube and a pair of valves; the valves are rotatably connected between the sampling tube and the cultivation tube's docking port; that is, when the valves are in a sealed pipe state, their longitudinal halves are located at the ports of the cultivation tube and the sampling tube, respectively, completely severing them. This invention, through the control section integrated into the cultivation tube, achieves leak-free and pollution-free in-situ sampling of the culture medium; through the synergy between an online early warning system composed of sensor probe assemblies and an offline micro-molecular detection instrument, it achieves a precise diagnosis of the deficiency of specific functional microorganisms, thus achieving the technical effect of accurately diagnosing the symbiotic state of bacteria and algae and providing a reliable basis for subsequent intervention.