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Haematococcus extract preparation, and preparation method and use thereof

A technology of Haematococcus pluvialis and extract, applied in the field of Haematococcus pluvialis extract preparation, can solve the problem of easy oxidation of astaxanthin, and achieve the effects of protecting easily oxidizable substances, simple operation and low energy consumption

Active Publication Date: 2011-02-23
YUNNAN GREEN A BIOLOGICAL PROJECT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, because the active ingredient astaxanthin in the extract of Haematococcus pluvialls is easily oxidized, the stability of the existing oral preparations of the extract of Haematococcus pluvialls needs to be further improved

Method used

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  • Haematococcus extract preparation, and preparation method and use thereof
  • Haematococcus extract preparation, and preparation method and use thereof
  • Haematococcus extract preparation, and preparation method and use thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0047] 3) Preparation of the test solution

[0048] (1) Sample solution ①: Weigh about 20 mg of the prepared Haematococcus pluvialis extract and weigh it accurately. Place in a 25mL volumetric flask, add a small amount of acetone and shake gently to dissolve, add acetone to make up to the mark, and shake well. Precisely draw 1mL into a 50mL volumetric flask, dilute to the mark with acetone, and shake well.

[0049] (2) Sample solution ②: Accurately measure 1.5 mL of the prepared sample solution ①, put it in a 10 mL centrifuge tube, add 1.0 mL of Tris-HCl, mix well, incubate in a 37°C water bath for 2 minutes, add 100 μL of cholesteryl ester Enzyme solution, mix well, fill the centrifuge tube with nitrogen for 15 seconds to remove the air, and seal the centrifuge tube mouth with a self-sealing membrane. Incubate in a 37°C water bath for 45-50 minutes, shaking the centrifuge tube every 15 minutes. Add 2.0mL petroleum ether and 0.5g sodium sulfate decahydrate, vortex vigorousl...

Embodiment 1

[0056] Example 1 Preparation of Haematococcus Extract

[0057] This embodiment adopts the supercritical CO provided by the present invention 2 The fluid extraction method prepares the Haematococcus pluvialis extract, specifically comprising the following steps:

[0058] 1) Broken wall of Haematococcus pluvialis

[0059] A. Wall breaking equipment

[0060] A high-pressure homogenizer is used for wall-breaking treatment.

[0061] B. According to the processing capacity and processing effect of the existing high-pressure homogenizer, the maximum processing capacity of algae mud at one time is 400-500kg (equivalent to 3000m 2 The total amount of algae sludge harvested by the culture system at one time).

[0062] C. The control parameters are shown in Table 2.

[0063] Table 2 Process control parameters of Haematococcus red spore breaking process

[0064]

[0065] D. In addition to meeting the requirements of the above control parameters during the process of breaking th...

Embodiment 2

[0085] This embodiment is the optimal determination process of the mass-volume ratio of the Haematococcus pluvialis extract and the external oil phase in the soft capsule provided by the present invention, which is divided into the following aspects to illustrate the problem:

[0086] A. The purpose of adding external oil phase to soft capsules prepared from Haematococcus pluvialis extract

[0087] The Haematococcus pluvialis extract is composed of functional components (astaxanthin) + its own oil phase, wherein the astaxanthin content is 5-10% (w / w). Due to the high content of astaxanthin and its instability, it is not suitable to be directly made into soft capsule products. Therefore, it is necessary to add an external oil phase. Its purpose and function are as follows:

[0088] (1) As a diluent: dilute the astaxanthin content in the extract to the specified concentration to ensure compliance with the soft capsule astaxanthin content specification;

[0089] (2) As a stabili...

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Abstract

The invention provides a haematococcus extract preparation, and a preparation method and use thereof. A haematococcus extract soft capsule provided by the invention comprises the contents of haematococcus extract and an oleic phase, wherein the astaxanthin content of the haematococcus extract is 2 to 20 percent (w / w); and the mass-to-volume ratio (g / mL) of astaxanthin in the haematococcus extract and the oleic phase is 1-10:100. The invention also provides the method for preparing the haematococcus extract soft capsules. Experiments show that the haematococcus extract soft capsule provided by the invention has stability remarkably superior to that of the haematococcus extract and conventional products and is of great importance to the application of the astaxanthin to antioxidation, the enhancement of immunity, the suppression of tumorigenesis, the prevention and treatment of cardiovascular disease, the prevention of skin aging and the maintenance of health of eyes and the central nervous system.

Description

technical field [0001] The invention relates to an extract preparation of Haematococcus pluvialis, its preparation method and application. Background technique [0002] Haematococcus is a single-celled green algae that can accumulate a large amount of astaxanthin under adverse conditions, and is recognized as the main source of natural astaxanthin in nature. Using supercritical fluid CO 2 The advanced Haematococcus pluvialis extract is not only rich in natural antioxidant astaxanthin, but also contains vitamins B1, B2, B6, B12, monounsaturated fatty acids, polyunsaturated fatty acids and other essential nutrients for human body. [0003] Astaxanthin is a red natural carotenoid, which has a powerful ability to scavenge oxygen free radicals. Its antioxidant activity is 550 times that of vitamins and 10 times that of carotenoids. It is known as a "super antioxidant" (1. Nakagawa K, Kang S, Park D, et al. Inhibition by beta carotene and astaxanthin of NADPH dependent mi crosom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P37/04A61P9/00A61K36/02A61K47/42A61P17/16A61K47/44A61P3/02A61P35/00A61K9/48A61P27/02A61K47/22A61P25/00A61K47/14A61P39/06A23L1/29A23L33/00
Inventor 龙祥胡志祥万庆家张秋玲
Owner YUNNAN GREEN A BIOLOGICAL PROJECT
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