5results about How to "Prevent oxidative denaturation" patented technology

The invention belongs to the technical field of biology, and particularly relates to a reagent combination and a kit for extracting nucleic acid through a paramagnetic particle method and application of the reagent combination and the kit. The reagent combination or the kit does not adopt beta-mercaptoethanol with pungent smell or dithiothreitol needing to be stored at low temperature as an antioxidant, but adopts tris (2-formylethyl) phosphine hydrochloride and / or metabisulfite as an antioxidant, belongs to common salt chemical reagent powder, can be stored at normal temperature, and can be used for preparing a reagent for the first time. Manual addition by experimenters before an experiment is not needed, so that nucleic acid (RNA) released by splitting decomposition can be effectively prevented from being oxidized and denatured, and the integrity of extracted nucleic acid (total RNA) fragments is ensured; especially, the metabisulfite is commonly used in food additives, does not cause harm to the body, and is non-toxic and non-volatile.

The invention discloses compact anti-wrinkle elastin peptide freeze-dried powder and a preparation method thereof, and belongs to the technical field of bioactive peptides. The freeze-dried powder is prepared by taking core tubes and / or throat tubes of bred animals as raw materials through cleaning and impurity removal, crushing and homogenizing, degreasing and impurity removal, composite enzymolysis, enzymolysis inactivation, centrifugal separation, gradient filtration, concentration, sterilization and vacuum freeze drying, and has remarkable elastinase inhibitory activity and tightening and anti-wrinkle effects. The livestock and poultry slaughtering byproducts which are wide in source and low in cost are selected as raw materials, high-value utilization of the livestock and poultry slaughtering byproducts is achieved, the optimized preparation technology solves the problems that in the prior art, enzymolysis efficiency is low, product purity is low, activity is insufficient, and stability is poor, the technology is simple, large-scale production is easy, and the method can be widely applied to the fields of cosmetics, food and health care products.

The invention discloses a bergamot pear polygalacturonase inhibitory protein extraction and purification method, which comprises: 1, crushing bergamot pears to obtain crushed bergamot pears; 2, precipitating the filtered crushed material by using an ammonium sulfate solution; 3, dissolving the precipitate with a buffer solution, and performing dialysis and primary purification to obtain a crude extract; and 4, purifying the crude extract by using a gel filtration chromatography column to obtain a purified extract, and drying to obtain the traditional Chinese medicine composition. The natural bergamot pear fruits are used as raw materials, and the method does not depend on chemical synthesis or genetic engineering technology, and accords with green, environment-friendly and sustainable development concepts; the extraction and purification process comprises multi-step purification processes such as filtration, precipitation, dissolution, dialysis and filter membrane filtration, and conventional laboratory technologies such as filter paper filtration, centrifugation and dialysis are used, so that the whole process is easy to implement and repeat, the feasibility and the reliability of experiments are improved, and the cost is reduced. The purity, the biological activity and the extraction rate of the extract are remarkably improved.

This invention discloses a porcine circovirus vaccine filling device, relating to the field of vaccine filling technology. The device includes a filling assembly comprising a base, a support frame on top of the base, the support frame being L-shaped, a servo motor at the top of the support frame, and a connecting frame fixedly mounted on the bottom telescopic end of the servo motor through the support frame. The connecting frame has several sets of mounting holes arranged sequentially, through which connecting pipes are installed. This invention, by setting up the filling assembly, can effectively buffer the filling process. The sieve plate provides effective buffering and defoaming, solving the problem of air bubbles easily generated during filling in existing devices. It avoids the problem of large differences in drug dosage due to air bubbles, ensuring the product complies with veterinary pharmacopoeia regulations. Simultaneously, it prevents oxygen in the air bubbles from accelerating the oxidative denaturation of vaccine antigen proteins, ensuring the drug's efficacy and preventing immunization failure.

Provided is a cinnamyl aldehyde-loaded solid-liquid biphasic artificial cell (SL-BACs) which has a solid-phase protective film and a liquid multicore, and which is efficiently and stably produced using a microfluidic T-type chip. The SL-BACs can provide multi-aspect treatment assistance for colitis induced by salmonella typhimurium. Firstly, an aptamer in a liquid multinuclear shows accurate targeting at an infected part; secondly, the encapsulation efficiency of the SL-BACs on the cinnamyl aldehyde is relatively high, and is about 82.9 percent; thirdly, the intestinal retention time of the SL-BACs is prolonged to 15 h through the membrane structure; in addition, the SL-BACs show pH responsiveness, liquid multi-core antibiosis is controlled to be released, and the sterilization rate is 95.28%. The SL-BACs can also reduce the secretion of proinflammatory cytokines, optimize the composition of intestinal microbiota and maintain the steady state of the intestinal tract. In a word, the SL-BACs provide necessary conditions for modularizing the artificial cells into an oral system for targeted therapy.