100 results about "Artificial cell" patented technology

In biology and biotechnology, electroporation is an important technique for introducing entities (DNA, RNAi, peptides, proteins, antibodies, genes, small molecules, nanoparticles, etc.) into cells. Applications range widely from genetic engineering to regenerative medicine to drug delivery. It has been demonstrated that the electrical currents flowing through cells can be used to monitor and control the process of electroporation for biological and artificial cells. In this application, a device and system are disclosed which allow precise monitoring and controlling electroporation of cells and cell layers, with examples shown using adherent cells grown on porous membranes.

An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.

The present invention relates to a method for culturing cells, which comprises the steps of: causing cells to adhere to the surface of a cell array substrate having a cell adhesiveness variation pattern that comprises regions having good cell adhesiveness and regions having inhibited cell adhesiveness patterned on a substrate; transferring the adhered cells to a cell culture substrate in such patterned state; and culturing the transferred cells.

The invention discloses an amphiphilic porous hollow carbon microsphere, a preparation method thereof, applications of the amphiphilic porous hollow carbon microsphere as a carrier in enriching and loading of active molecules or a catalyst, and a method for separating and recovering the porous hollow carbon microsphere from a dispersion system by utilizing the amphipathy. The method comprises thefollowing steps of: dispersing microzyme in an aqueous solution of a cytoderm surface protectant; and then, carrying out carbonation treatment to obtain the amphiphilic porous hollow carbon microsphere. The prepared hollow carbon microsphere has a pore size capable of being controlled in the mesoporous and macroporous range as required, and can be suitable for the loading, the storage and the transmission of guest molecules in different sizes; by utilizing the characteristic of the amphiphilic porous hollow carbon microsphere capable of spontaneously transferring to a two-phase interface froma polar or non-polar dispersion system, the microsphere can be conveniently separated and recovered from a reaction mixing system. The easy and low-cost synthesis method and the special properties ofthe hollow carbon microsphere are favorable to the applications of the hollow carbon microsphere to the fields, such as energy storage, medicament transmission, artificial cells, catalytic carriers, and the like.

A method for the treatment of spider silk filament for use as a thread or composition in the manufacture of cosmetic, medical, textile, and industrial applications, wherein the spider silk filament, derived from genetically modified organisms, is treated with at least one component selected from the group consisting of vitamins, hormones, antioxidants, chelating agents, antibiotics, preserving agents, fragrances, dyes, pigments, magnetic nanoparticles, nanocrystals, cell adhesion enhancers, thermal insulators, shrinkage agents and cosmetic, medical or dermatological active substances. Textile fabrics obtained by this method are stronger, bio-compatible, bio-degradable and have a higher thermal conductivity. Treated spider silk filament can also be applied in an oil-in-water or water-in-oil protective cream that is hypoallergenic and ensures a firmer skin.

The invention discloses artificial cell membrane materials applied to photoinduction stem grafting and a synthesis method of the artificial cell membrane materials. The synthesis method of the artificial cell membrane materials comprises the following steps that 2-(dodecyl trithiocarbonate)-2- methyl propionic acid, dicyclohexyl carbodiimide, 4- dimethylamino pyridine and dichloromethane are added into a flask, all the components are stirred and dissolved under the protection of nitrogen, azide ethanol is dropwise added and stirred, washing is carried out through diluted hydrochloric acid and distilled water, solvents are removed through a reduced pressure distillation method, and a yellow oily liquid a is obtained; MPC, a midbody a, azo different nitrile and absolute ethyl alcohol are added into a boiling tube and are evenly dissolved, then after the nitrogen is removed through three times of 'vacuumizing-induction of highly pure nitorgen' operations on the boiling tube, and reaction away from light is carried out on the boiling tube in a sealed mode under the protection of highly pure nitrogen; the reaction liquid is precipitated through trichloromethane/diethyl ether mixed solvents, and solid products are collected. By means of the technical scheme, the artificial cell membrane materials have good biocompatibility, achieve the various effects of restraining of protein adsorption, anticoagulation, surface lubrication and the like, and have good medical prospects.

The cryopreservation of cells and tissue cultures for long term storage of cells, cell constructs or three-dimensional complex tissues assemblies is based on the use of a collagen cell carrier (CCC) having a specific composition and thickness and appropriate mechanical properties which are maintained after thawing. The collagen cell carrier provides a suitable support for the cryopreservation resulting in high survival rates after thawing and providing cells and tissue assemblies already adhered to a mechanically stable and biocompatible support. Frozen collagen carrier-cells assemblies, the frozen artificial cell constructs or three-dimensional complex tissue assemblies obtainable by the method and the use thereof after thawing are also disclosed.

The present invention features a freestanding microfluidic pipette with both solution exchange capability and fluid re-circulation, enabling highly localized and contamination- free fluid delivery within a confined volume in the vicinity of the pipette exit. Preferably, the device features direct positioning, so the pipette can be directed at a point or object of interest, such as a biological or artificial cell, a defined surface area or a sensor element within an open volume, using micro- or nanopositioning techniques. One aspect of the invention provides a free-standing pipette. The free-standing pipette includes a microfluidic device comprising one or more channels with exits leading into an open-volume and a positioning device programmed to hold the microfluidic device at an angle from a horizontal axis.

The invention provides a preparation method of a liposome vesicle based on a microfluidic device. The preparation method comprises the steps of: preparing a microfluidic chip by a soft lithography method; preparing a precursor solution for preparing the liposome vesicle; and using a constant-pressure injection pump to inject the precursor solution into a ''Y''-shaped chip through different injection ports, setting a total flow rate, adjusting the flow ratio of a buffer solution to a phospholipid molecular alcohol solution, and collecting a product at an outlet, wherein the product is the prepared liposome vesicle. The microfluidic chip selected by the invention is a ''Y''-shaped microfluidic chip with a serpentine mixing channel of a square structure, and the chip is prepared by the soft lithography method. By using the chip, based on the principle of self-assembly on an interface, by adjusting the total flow rate and the flow rate ratio of the aqueous buffer solution to the phospholipid molecule ethanol solution, regulation of the size of the liposome vesicle and control on the dimensional uniformity are achieved, and an efficient low-cost method is provided for preparation of artificial cells with uniform properties as well as drug carriers.

A liposome preparation of ribavirin used as an antivirus medicine is disclosed. Said liposome preparation is the artificial cells, which can carry the ribavirin into the cells infected by virus, resulting in high effect and low toxin. It is prepared through dissolving ribavirin in the buffering solution of phosphate, adding the mixture of phosphatide, cholesterin and VE, heating to 58-62 deg.C, and maintaining the temp for 30 min to obtain uniform emulsion.

Intended for the use in neurosurgery and organ tissue engineering to replace defects of nervous tissue of a mammal brain and spinal cord (B&SC) in reconstructive neurosurgical operations, a tissue-replaceable artificial cell-biopolymer neuroendoprosthetic system (ACBP NEPS) for surgical plasty of defects of nervous tissue of B&SC, stimulating regeneration and growth of damaged axons of neural cells, comprises an elastic cell-biopolymer biologically active mass, produced from a heterogeneous collagen-containing matrix for implantation, and a biocomposition of cell preparations of various types of autologous cells of a patient. Also disclosed are a method of producing the ACBP NEPS, which provides for perfusion of the biocomposition of the cell preparations of various types of autologous cells of a patient into a heterogeneous collagen-containing matrix for implantation, and a method of reconstructive neurosurgical operation comprising implantation of the ACBP NEPS into a defect of neural tissue.

The invention relates to a button cell detecting, labelling and packaging all-in-one machine. The machine comprises an installing table, an input table fixed to the installing table, a conveyor belt arranging assembly, a cell transporting mechanism I, a labelling mechanism, a cell transporting mechanism II, a rotary packaging mechanism and packaging box jacking mechanisms. A cell input mechanism,a cell detecting mechanism and a cell transferring mechanism are arranged on the input table, one side of the input table is provided with an input belt aligned to the cell pushing mechanism, button cells are evenly arranged on the conveyor belt, cell storage boxes are stacked on jacking plates of the packaging box jacking mechanisms on two side faces of the rotary packaging mechanism, and shielding paper scraps are stacked on a jacking plate of the packaging box jacking mechanism at the third side face. Automatic detection, label sticking, boxing and packaging of button cells are achieved, the existing mode of artificial cell detection, labeling and packaging is replaced, the producing packaging efficiency of the button cells is improved, and the production quality of the button cells isimproved.

The invention provides a polypeptide nano-material containing double-pyrene groups as well as a preparation method and an application of polypeptide nano-material. The polypeptide nano-material containing the double-pyrene groups has a structure represented as a formula I; the polypeptide nano-material containing the double-pyrene groups can be deformed for self-assembly and can be self-assembled to form a nanofiber network structure in a tumor area and construct an artificial cell extracellular matrix in situ, and the artificial cell extracellular matrix selectively adheres to the tumor area to form a migration barrier, so that tumor invasion and metastasis can be effectively inhibited, and the polypeptide nano-material has board medical application prospect.

The invention relates to a preparation method of thylakoid-containing artificial cells and a photosynthesis simulating method, and belongs to the fields of biotechnology, biochemistry and synthetic biology. The method comprises the following steps of extracting thylakoid particles from fresh spinach leaves, and storing the thylakoid particles in a buffer solution for use; smearing a phospholipid solution onto an ITO glass electrode; after the solvent volatilization, forming a layer of phospholipid membrane; fixing a polytetrafluoroethylene framework by vacuum grease; adding liquid phases intothe polytetrafluoroethylene framework; applying alternating current onto the ITO glass electrode for preparing a GUV solution; mixing the GUV solution and a thylakoid solution; preparing the thylakoid-containing artificial cells. The thylakoid-containing artificial cells are prepared through the enlightenment of the natural plant cell structure and function; the model can realize the optical driveelectron transfer process under the artificial sunlight irradiation. Through the model building, the attributes of chloroplasts in nature are doubly simulated from the structure and the function. Thecomplicated cells realize simplified research, so that the development and the progress of the biomimetic chemistry are promoted.

The invention discloses a method for improving chitosan membrane blood compatibility through one-step coating at room temperature. The method includes: at the room temperature, dissolving phosphorylchloline polymer containing epoxy group in a polar solvent to obtain a polymer solution; adding ethylenediamine into the polymer solution, and mixing well to obtain a mixed solution; coating the mixed solution on the surface of a chitosan membrane, and sun-drying to obtain the chitosan membrane with an artificial cell outer layer membrane structure on the surface. The mixed solution containing the phosphorylchloline polymer and ethylenediamine is coated on the surface of the chitosan membrane at the room temperature, and the chitosan membrane with the artificial cell outer layer membrane structure can be prepared after sun-drying. The method is simple and mild in condition, and adhesion, to blood platelets, of the chitosan membrane after being improved through a one-step coating method is lowered obviously, and biocompatibility is improved remarkably.

The invention discloses a method for constructing artificial cells based on Janus magnetic nanoparticles and a DNA directional immobilization technology, and belongs to the field of artificial cell preparation. The preparation method comprises the following steps: firstly, preparing single-stranded DNA modified Janus magnetic nanoparticles and complementary single-stranded DNA functionalized glucose oxidase and horseradish peroxidase; mixing the Janus particles with an enzyme, and realizing immobilization of the enzyme through DNA complementary hybridization; jointly incubating immobilized double enzymes and tannic acid, realizing encapsulation of the immobilized enzymes through self-polymerization of the tannic acid, and constructing the artificial cell with a compartment structure. The artificial cell prepared by the method is simple in preparation process, mild in condition, high in internal enzyme activity, easy to separate from a reaction system and excellent in stability, and canbe used for simulating cascade reaction in natural cells.

The present invention provides Claudin-18.2-specific immunoreceptors (T cell receptors and artificial T cell receptors (chimeric antigen receptors; CARs)) and T cell epitopes which are useful for immunotherapy.

The invention relates to a micro-channel rapid biological particles counting method and belongs to the bioanalysis field. The method comprises the following steps: the biological particles to be detected are fixed and pass a micro-channel tube line at a constant flow rate, absorption peak number is recorded by a UV-VIS optical detector and the concentration C biological particles (number/ml) of the particle solution is computed, and the C biological particles equal N/ST, wherein, T is the detection time, N is the peak number recorded within T, and S is the flow rate of a pump. The biological particles counting method is simple and rapid, and can accurately compute cell concentration and cell count, which causes the traditional artificial cell activity observation and judgment to obtain a magnitude standard and helps realize quantitative analysis.

The invention provides a liquid metal artificial cell which comprises a semipermeable membrane, liquid metal and electrolyte, wherein a closed cavity is defined by the semipermeable membrane, the liquid metal and the electrolyte are located in the cavity. The invention further provides a preparation method for the liquid metal artificial cell. According to the liquid metal artificial cell, the technological concept of liquid metal artificial cell is introduced for the first time, a composite component system integrating the semipermeable membrane, the liquid metal, the electrolyte and more function materials into one is created, the existing knowledge of artificial cells and liquid metal is expanded significantly, the functions of the cells in the natural world can be simulated partly, and the liquid metal artificial cell can be further used for assembling an artificial machineswith more complex functions.

The invention provides a construction method of a tissue engineered tissue based on an artificial cell. The method comprises the following steps of: floating a cell suspension in a filtered and sterilized sodium alginate solution, and dropping the cell suspension into a calcium chloride solution through a dropping liquid generation device to form a calcium alginate bead; placing the bead into a membrane material contained solution, and coating a membrane on the surface of the bead to form a microcapsule, wherein the bead is cleaned by using normal saline; then, neutralizing superfluous positive charges on the surface of the microcapsule; liquefying the calcium alginate bead in the microcapsule by using a sodium citrate solution to form the artificial cell; next, curing and forming a mixture of the artificial cell and an aquogel to finally form an artificial cell and aquogel complex; and placing the artificial cell and aquogel complex under a static or oscillatory or microgravity condition to culture to construct the tissue engineered tissue. By using the construction method, the requirement of the traditional support for high porosity and high connectivity rate can be reduced; and the construction method is simple and beneficial to the realization of large-scale production for in-vitro construction.