32 results about "Artificial cell" patented technology

The invention discloses a preparation method of an artificial cell coated with a macrophage membrane, and belongs to the technical field of artificial cell preparation. The preparation method comprises the following steps: dissolving poly (diallyldimethylammonium chloride) and sulfobutyl-beta-cyclodextrin in ultrapure water, and mixing an aqueous solution of the poly (diallyldimethylammonium chloride) and an aqueous solution of the sulfobutyl-beta-cyclodextrin to obtain a PDDA / SBE-beta-CD condensation liquid droplet solution; and mixing the macrophage membrane suspension and the PDDA / SBE-beta-CD condensed fluid drops in an equal mass ratio, and realizing in-situ coating of the macrophage membrane by adopting an ultrasonic coating technology to obtain the artificial cell coated with the macrophage membrane. The PDDA / SBE-beta-CD condensation liquid droplet constructed by the invention has good stability under the conditions of physiological salt concentration and pH and at 37 DEG C; the artificial cell coated with the macrophage membrane is prepared by mixing with the macrophage membrane suspension, and the macrophage membrane serving as a shell not only can prevent aggregate aggregation, but also can improve the structural stability and biocompatibility.

The present invention addresses the problem of providing a composition for lyophilizing extracellular vesicles or artificial cell vesicles, which makes it possible to prevent the extracellular vesicles or the artificial cell vesicles from being deteriorated in shape preserving properties or functions thereof or from being reduced in particle diameters when the extracellular vesicles or the artificial cell vesicles are recovered by hydration after lyophilization. The composition for lyophilizing extracellular vesicles or artificial cell vesicles is prepared, the composition being characterized by being intended to be added to extracellular vesicles or artificial cell vesicles upon use and also being characterized by containing the following hydrophobic substances: (a) phenylalanine or a methyl ester thereof; (b) a dipeptide which is composed of one or two kinds of hydrophobic amino acids selected from the group consisting of phenylalanine, leucine, glycine, valine, isoleucine, tryptophan, and alanine and contains at least one phenylalanine molecule as a constituent amino acid; and the like.

The application discloses a kind of artificial cells for single-cell mass spectrometry and preparation method thereof, wherein artificial cell includes internal water phase, intermediate oil phase and external water phase;Internal water phase includes polyethylene glycol and polyvinyl alcohol aqueous solution, intermediate oil phase includes L-alpha-phosphatidylcholine chloroform and hexane mixture;External water phase includes PVA and F-68 aqueous solution.Compared with natural cell sample, the novel artificial single cell based on microfluidic self-assembly developed in the application has better uniformity, stability and controllability, effectively avoids significant measurement difference between single-cell individual samples, and effectively solves the problem of difficult stable preservation of biological samples.The proposed artificial cell preparation method will largely solve the problem of lack of standard reference material in the field of single-cell mass spectrometry, making the results of single-cell mass spectrometry method more accurate, reliable and mutually recognized.

The invention discloses a multi-chamber vesicle as well as a preparation method and application thereof. Each multi-chamber vesicle is an anionic liposome, and a cavity of the anionic liposome contains a plurality of cationic liposomes and a buffer solution containing Mg < 2 + >. According to the preparation method of the multi-chamber vesicle, a special oil phase and a water phase with different charges are introduced, so that the multi-chamber vesicle in which a plurality of cationic liposomes are wrapped by a single anionic liposome is successfully prepared. The multi-chamber vesicle is good in biocompatibility, controllable in particle size and stable in mechanical property, and provides technical support for preparation of artificial cells and novel drug carriers in the future.

The invention belongs to the field of biomedical engineering, and particularly relates to an amino acid modified molybdenum disulfide artificial extracellular matrix and application. Preparing an amino acid modified molybdenum disulfide artificial extracellular matrix, inoculating the neuron in-vitro model PC12 cell suspension onto the artificial extracellular matrix, and constructing the nerve cell in-vitro differentiation model. The artificial extracellular matrix with good biocompatibility is obtained by constructing different kinds of amino acid modified molybdenum disulfide nano material artificial extracellular matrixes as the artificial extracellular matrix of nerve cells PC12, and the artificial extracellular matrix improves the activity of the PC12 cells and promotes the differentiation of nerve cell synapses; a neuron in-vitro model with high differentiation and higher activity is constructed, and the artificial extracellular matrix can be used for nerve regeneration and neural engineering.

The utility model relates to the technical field of protein extraction, and discloses a protein extraction scraper device which comprises a base, a scraper assembly is arranged at the top of the base, the scraper assembly comprises fixing plates, a sliding groove, a rotating shaft, a first connecting rod, a second connecting rod, a connecting plate, a scraper mechanism, a guide rail and a sliding block, and the fixing plates are arranged on the two sides of the top of the base; sliding grooves are formed in the fixing plates, a rotating shaft is arranged between the two fixing plates, and first connecting rods are arranged at the two ends of the rotating shaft. According to the protein extraction scraper device, by arranging the scraper assembly, manual cell scraping can be replaced, traditional cell scraping is replaced, the labor intensity of workers is reduced, a driving motor is started to be matched with a rotating shaft, a first connecting rod, a second connecting rod and a connecting plate, and a scraper mechanism can be driven to move downwards; the stepping motor is started to drive the scraper blade to rotate, and bottom cells of the culture dish are scraped.

The present invention provides Claudin-6-specific immunoreceptors (T cell receptors and artificial T cell receptors (chimeric antigen receptors; CARs)) and T cell epitopes which are useful for immunotherapy.

The invention provides an assembling mode of RNA liquid drops deformed through photostimulation and application of the RNA liquid drops, and belongs to the technical field of preparation of nucleic acid for analysis. The method comprises the following steps: mixing an RNA single strand with a buffer solution through structural design to obtain a mixed system with a specific concentration; after the mixed system is subjected to heat shock to complete base pairing and assembling, sealing and packaging are performed, the RNA liquid drops deformed through photostimulation are obtained, and the diameter of the liquid drops is 7.0-13.0 microns. The RNA liquid drop obtained in the embodiment can deform under light stimulation, behavior regulation and control can be achieved through group modification, and feasibility is provided for construction and functional expression of artificial cells.

The invention discloses a preparation method of a bionic system based on a microfluidic technology, and relates to the technical field of functional bionic materials. Chitosan, nano-enzyme, alginate, phytic acid and the like are taken as raw materials, the artificial cell system with adjustable morphology, structure and activity is prepared in a high-throughput manner by utilizing a micro-fluidic chip through an intermolecular polymerization reaction, and the prepared artificial cell system can simulate a glucose metabolism function.

The invention relates to a carbon nanotube embedded artificial cell, a preparation method thereof and an application of the carbon nanotube embedded artificial cell in degradation of antibiotic resistance genes, and belongs to the technical field of environmental functional materials. According to the material, an artificial cell membrane formed by lipidosome serves as a shell, layered double-oxide hollow spheres derived from ferrocobalt layered double hydroxides are packaged in the shell, and carbon nanotubes are embedded into the artificial cell membrane to serve as a transmission channel. The preparation method comprises the following steps: preparing carbon spheres by a hydrothermal method, preparing ferrocobalt layered double hydroxides by a coprecipitation method, carrying out composite calcination to obtain layered double oxide hollow spheres, and finally constructing artificial cells by a film hydration method and embedding the artificial cells into the carbon nanotubes. The material can be used for catalytically degrading antibiotic resistance genes in water, singlet oxygen and superoxide free radicals are generated through peroxymonosulfate activation, gene segments are effectively attacked, and efficient degradation is achieved. The method has the advantages of efficient catalytic activity and confinement encapsulation, magnetic recovery is facilitated, and a novel environment-friendly method is provided for treatment of antibiotic resistance genes.

The invention discloses an artificial cell and a preparation method thereof as well as a bionic tissue and a preparation method thereof, an aqueous dispersion of a negative electricity nanometer material is sprayed into an aqueous phase receiving bath containing a cationic polyelectrolyte and a surfactant through a gas-liquid shearing microfluidic technology, and one-step construction of an artificial cell membrane and a cytoskeleton is realized. According to the method, the internal skeleton enhanced artificial cell is constructed in a water phase by a one-step method by utilizing the electrostatic interaction of the negative electricity nano material and the cationic polyelectrolyte, and the method has the characteristics of convenience in preparation and excellent stability. By introducing different functional materials, multifunctional integration of the artificial cell can be realized. Meanwhile, the adhesion inducer is added, so that a bionic tissue can be further constructed, and the bionic tissue integrates excellent mechanical properties of the artificial cells and can bear disturbance to a certain degree without disintegration.

The present application relates to a kind of extracellular matrix-cell complex for inhibiting intervertebral disc degeneration and its preparation method and application, the extracellular matrix-cell complex for inhibiting intervertebral disc degeneration includes modified cell and the biomimetic extracellular matrix nanofiber constructed on the surface of modified cell.The present application is creatively modified to implant cell in cell therapy, constructs artificial extracellular matrix with nanofiber characteristics in situ on the surface of cell, the complex is implanted into nucleus pulposus by cell injection therapy, can induce cell rest, so that implanted cell is better adapted to the intracellular environment of nucleus pulposus anoxia.The artificial extracellular matrix can be long-term targeted to cell surface, significantly prolongs the action time, and different modified cells can be selected according to actual needs, so that intervertebral disc degeneration cell therapy can be better realized, with wide application prospect.

The invention belongs to the technical field of functional nano materials and biomimetic catalysis, and discloses a universal preparation method and application of high-entropy nano enzyme. According to the method, various metal salts, polyvinylpyrrolidone, benzyl alcohol and formaldehyde are used as raw materials, and various high-entropy alloy nanoparticles and nanosheets are prepared under the same reaction condition through a solvothermal method. The prepared high-entropy nano-enzyme has the advantages of controllable composition, uniform morphology, high catalytic activity and the like, can simulate the activity of various natural enzymes, and is suitable for the fields of artificial cell systems, biological catalysis, sensing and the like.

The application of carbon nanotubes in miRNAs detection and cell imaging, the application uses carbon nanotube nanomaterial as an effective artificial transmission channel, promotes the signal and ion transmission of liposome artificial cell to cell membrane. The addition amount of carbon nanotubes will affect the efficiency of membrane fusion, the application studies the addition amount of carbon nanotubes and the ion channel function of carbon nanotubes in the whole system.

Some embodiments provide for a method of using a trained machine learning model to predict a characteristic of a subject, the method comprising: processing cfRNA expression data using the trained machine learning model to obtain an output indicative of the characteristic of the subject, wherein the trained machine learning model was trained using artificial cfRNA expression data, the artificial cfRNA expression data comprising a plurality of artificial cfRNA expression profiles, an artificial cfRNA expression profile having been generated by: generating a healthy expression profile component; generating a tumor expression profile component; and generating the artificial cfRNA expression profile using the healthy expression profile component and the tumor expression profile component.

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.

The invention discloses a device suitable for aerosol phase virus-receptor affinity and activity evaluation, an evaluation method and a data analysis method. The device comprises an aerosol generation unit, a bionic artificial cell array unit, an environment control and fixing assembly and an activity evaluation and collection unit. The method is characterized in that through a micro-nano processing and molecular self-assembly technology, a supporting lipid double-layer membrane is constructed on an array substrate, receptor protein is directionally fixed, and a bionic cell interface is formed. When in use, a virus sample is atomized into aerosol and flows through the interface, so that the virus is specifically combined with the receptor; affinity parameters in an aerosol environment can be obtained by combining quantitative detection with viruses and utilizing algorithm model fitting. Meanwhile, the system can directly collect an aerosol sample after action and inoculate cells for culture, so that synchronous verification of virus biological infection activity is realized. The device is easy and convenient to operate and high in universality, and a brand new tool is provided for studying the recognition and infection mechanism of viruses under the real aerosol transmission condition.

The present invention relates to a method for manufacturing a three-dimensional structure based on a block copolymer. The method comprises the steps of injecting a block copolymer (BCP) solution into each micro-well formed on the substrate and drying it to form a block copolymer layer, and applying a buffer to the block copolymer layer to hydrate the micro-well in three dimensions Forming the structure, after the three-dimensional structure is formed, injecting and curing a hydrogel solution around the three-dimensional structure may include the step of enhancing stability. In particular, the process of hydration by applying a buffer to the micro-well is performed while an electric field is applied. By controlling the concentration of the block copolymer (BCP) and the amplitude and frequency of the electric field, a three-dimensional artificial cell membrane having a desired size and shape, such as a spherical or ciliary shape and high stability (100% survival for 50 days) is manufactured can do. The present invention can be efficiently applied to various biological fields such as artificial cells, cell-mimicking biosensors, and bioreactors.

The invention discloses an artificial cell capable of transferring nano-scale particles and a preparation method and application thereof, the artificial cell comprises self-expanding oil drops and a pore-forming agent, and the artificial cell is formed through hydrolytic condensation, heterogeneous nucleation and ultraviolet polymerization, the self-expanding oil drops are inorganic precursors with rich siloxane bonds and polymerizable methacrylate bonds, and the pore-forming agent is a high-molecular compound capable of being selectively removed. The surface of the artificial cell is provided with a plurality of nano-scale micropores, so that a transfer channel can be provided for nano-scale particles loaded in the artificial cell, and a new thought is provided for constructing a more bionic artificial cell.

The invention discloses an artificial cell wall system based on a DNA logic gate and application of the artificial cell wall system, the artificial cell wall system comprises a probe 1 (Apt-S-T1) and a probe 2 (T2-S-Apt), Apt is a nucleic acid aptamer targeting a to-be-treated cell membrane receptor, S is a spacer region sequence, and T1 and T2 are a first toe end sequence and a second toe end sequence respectively; a linker; the hairpin comprises a hairpin body 1, a hairpin body 2 and a cross-linking agent, and the tail end of the hairpin body 1 or the tail end of the hairpin body 2 is modified with a hydrogel framework material. Artificial cell walls are constructed based on a DNA logic gate, the integrity of cell membranes is protected in the freezing process, and DMSO-free cryopreservation is achieved; and the resuscitated cell has a biological activation function, so that the resuscitated cell not only can survive, but also has a proliferation capacity superior to that of a fresh cell. Besides, the cell cryopreservation method based on the artificial cell wall system has universality, corresponding artificial cell wall systems can be customized according to different cell types, and cryopreservation requirements of various cell types are met.

Provided is an ion current measurement system capable of easily identifying whether an artificial cell membrane chip is to be used for the first time or has already been used. Specifically, the ion current measurement system measures an ion current flowing through an ion channel formed in an artificial cell membrane, using an ion current measurement device for an artificial cell membrane chip including at least one well forming the artificial cell membrane. The artificial cell membrane chip includes a display section at a predetermined site. The ion current measurement device includes an identification means. The identification means recognizes the display section to identify whether the artificial cell membrane chip is to be used for the first time or has already been used.

The application discloses a preparation method of macrophage membrane coated artificial cells and belongs to the technical field of artificial cell preparation. Polydiallyldimethylammonium chloride and sulfobutyl-beta-cyclodextrin are dissolved in ultrapure water, and the aqueous solutions of the two are mixed to obtain a PDDA / SBE-beta-CD condensate droplet solution; equal-mass-ratio macrophage membrane suspension liquid and the PDDA / SBE-beta-CD condensate droplet solution are mixed in equal ratio, in-situ coating of the macrophage membrane is realized by using ultrasonic coating technology, and macrophage membrane coated artificial cells are obtained. The PDDA / SBE-beta-CD condensate droplet constructed by the application has good stability under physiological salt concentration, pH conditions and 37 DEG C; the macrophage membrane coated artificial cells are prepared by mixing the macrophage membrane suspension liquid, and the macrophage membrane as the shell can not only prevent the condensate from gathering, but also increase the structural stability and biocompatibility.

To provide a technique for suppressing excessive scarring of a biological tissue in a wound healing process.SOLUTION: An artificial scaffold material for wound applied to wound of a living body contains a biological absorbing biomaterial and an anti-fibrosis agent as main components, wherein the biomaterial contains the anti-fibrosis agent at least during usage of the scaffold material for wound. The biomaterial functions as an artificial extracellular matrix having properties to bond a precursor cell of a myofibroblast of a living body, in a wound. The scaffold material for wound is placed so that it comes in local contact with wound formed on the inner surface of a closed lumen in the living body, during usage. Thereby, the anti-fibrosis agent and the biomaterial are locally administrated into the wound, and thereby in a wound healing process, anti-scarring treatment is conducted in an anti-scarring treatment delaying suppression state.SELECTED DRAWING: Figure 4

The invention relates to the technical field of functional material preparation, in particular to a multi-layer composite liquid metal artificial cell as well as a preparation method and application thereof. The preparation method provided by the invention comprises the following steps: injecting part of an electrolyte solution into a mold, and carrying out first freezing to obtain a cooled and cured solution layer; a pre-cooled liquid metal solid-phase ball is placed on the upper surface of the cooling solidification solution layer, second freezing is carried out, the mold is filled with the remaining electrolyte solution, third freezing is carried out, and a solution-liquid metal double-layer ice ball is obtained; and taking out the solution-liquid metal double-layer ice ball, putting the solution-liquid metal double-layer ice ball into a hydrogel precursor solution, and then carrying out a cross-linking reaction to form a hydrogel outer membrane. According to the multi-layer composite liquid metal artificial cell and the low-temperature layering preparation method, the manufacturing problem of a multi-layer composite structure and the like is solved, the liquid metal is separated from a large-range solution, the characteristics are guaranteed through multi-layer coating, the multi-layer composite liquid metal artificial cell is expanded and applied to air, and a new way is provided for tiny liquid drop conveying and directional embolism.

The application belongs to the field of biomedical engineering, and particularly relates to an amino acid modified molybdenum disulfide artificial extracellular matrix and application. The amino acid modified molybdenum disulfide artificial extracellular matrix is prepared, neuron in vitro model PC12 cell suspension is inoculated on the artificial extracellular matrix, and a nerve cell in vitro differentiation model is constructed. The application constructs different kinds of amino acid modified molybdenum disulfide nanomaterial artificial extracellular matrix for nerve cell PC12 cell artificial extracellular matrix, obtains artificial extracellular matrix with good biocompatibility, the artificial extracellular matrix improves PC12 cell activity and promotes differentiation of nerve cell synapse, constructs a neuron in vitro model with high differentiation and higher activity, and the artificial extracellular matrix can be used for nerve regeneration and nerve engineering.

The present invention provides closed protein-18.2 specific immune receptors (T cell receptors and artificial T cell receptors (chimeric antigen receptors; CARs)) and T cell epitopes for immunotherapy.

The invention particularly relates to an enzyme immobilized hollow multi-shell structure bionic artificial cell for accurately detecting cholesterol, which is characterized in that the artificial cell comprises a metal oxide / carbonate hollow multi-shell structure, an enzyme and an artificial cell membrane, the artificial cell membrane is uniformly distributed on the surface of the hollow multi-shell structure, and the enzyme is uniformly loaded in an internal cavity. The simple and safe preparation method of the artificial cell membrane can avoid toxicity and mechanical damage in a traditional coating method, and the layer-by-layer nested hollow multi-shell structure realizes structural simulation of cells and endows the cells with selective permeability and long-term stability of enzyme. The artificial cell has efficient mass transfer performance, accurate and rapid detection of cholesterol in a solution and serum is realized through enzymatic cascade catalytic reaction, the detection range is 50-300 [mu] M, and the lowest detection limit is as low as 4.9 [mu] M. The method can be applied to serum cholesterol detection, cholesterol detection is achieved through an artificial intelligence method based on an image recognition model, and the method is expected to be used for convenient detection of cholesterol.

The present invention relates to viral vectors co-encoding an artificial CD4 T cell epitope (PADRE) for enhanced immune responses to encoded multimeric protein particle displayed vaccine antigens.

Provided is a method for forming an artificial cell membrane chip, the method making it possible to efficiently create a plurality of wells. Specifically, the method for forming an artificial cell membrane chip is for forming an artificial cell membrane chip provided with a plurality of wells that form an artificial cell membrane, the method including: a step for preparing a substrate in which a plurality of recesses are provided in a row in a plate-shaped substrate, and a groove for connecting the recesses to one another is formed between adjacent recesses in the row; and an insertion step for inserting band-shaped sheets into the recesses and the groove. In the insertion step, one of the sheets is inserted into at least two of the recesses and the groove between said recesses, and the sheet separates the recesses into two portions and is provided with a through hole for communicating the two portions. The wells comprise the sheet and the recesses separated into two portions by the sheet.

Provided is an analysis method whereby an analysis can be performed in a short time by appropriately providing a means of analyzing an enormous amount of calculations required for analyzing current data. Specifically, an analysis method for an ion current flowing through an ion channel comprises: an ion current measurement step of measuring an ion current for at least one artificial cell membrane chip; an ion current data storage step of storing the measured ion current data; a transmission step of transmitting the stored ion current data to a server via a network; an analysis step of analyzing the ion current on the server by a predetermined analysis method; and an analysis result storage step of storing the analyzed analysis result on the server, wherein the ion current measurement step and the ion current data storage step are executed on ion current measurement devices, and the server is connected to the at least one of the ion current measurement devices via the network.