30 results about "Transfer cell" patented technology

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

A composite transfer cell and associated components and methods are presented. The composite transfer cell comprises a delivery system on a first side of the composite transfer cell; a tool on a second side of the composite transfer cell; and a composite transfer system between the first side and the second side of the composite transfer cell, the composite transfer system comprising a plurality of flipper arms independently movable in a vertical direction and rotatingly movable to lift and place a composite charge from the delivery system to the tool.

A cell transfer apparatus transfers cells scattered in a first container to a second container. The cell transfer apparatus includes a head with a suction tip, a movement mechanism for the head, a camera that captures images of the first container, a cell recognition unit that performs position recognition of the cells based on the images, and a control unit that controls the head and the movement mechanism to perform a transfer operation of the cells based on a result of the position recognition. The control unit gradually suctions the set quantity of target cells that is set to two or more in advance into the suction tip from among the cells scattered in the first container, causes the head to move to the second container, and then discharges the set quantity of the target cells suctioned into the suction tip to the same location in the second container.

The present disclosure relates to a connection port (1) for a tight transfer cell, this port comprising a flange (2) extending about a main axis (AX), this flange (2) comprising an outer face (7) and an inner face (8) which is equipped with a door (3) movable between an open position and a closed position wherein this door (3) seals a central opening (6) of the flange, and a slide (14) rigidly secured to the door (3) and passing through the flange (2), the slide (14) being connected to the flange (2) by a kinematic connection with a parallel axis (AX1) to the main axis (AX). The kinematic connection comprises a slide connection portion for applying a translational movement to the slide (14) along the axis (AX1) on a first displacement part, and a helical connection portion for applying a helical movement to the slide (14) about the axis (AX1) on a second displacement portion.

The invention discloses a liquid path system and an automatic needle changing structure for micromanipulation, and relates to the technical field of micromanipulation. The liquid suction and spitting structure aims to solve the problem that gas in an air cavity of an existing liquid suction and spitting structure is prone to being compressed, and the deviation between liquid relief precision and expectation is too large. The liquid path system comprises a liquid driving unit, the liquid driving unit is detachably connected with a liquid inlet of a micromanipulation needle of a micromanipulation automatic needle changing unit, and incompressible liquid is injected into the micromanipulation needle, so that a continuous liquid column without an air cavity is formed between the liquid driving unit and the micromanipulation needle; liquid is used as a medium to suck and spit cells or tissue fragments, and perfusion or cleaning is carried out; the automatic needle changing structure comprises a liquid path system and a micromanipulation automatic needle changing unit, and the liquid path system is connected with the micromanipulation automatic needle changing structure and used for transferring cells or tissue fragments with liquid as a medium in micromanipulation. The method is used for cell culture and organoid culture.

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

This disclosure relates to a connection port for a sealed transfer cell, this port comprising a flange (2) having an outer face (8) and an inner face (7) which carries a hinge (4) to which a door (3) movable between an open and a closed position is attached. The hinge (4) has two fixed bearings which jointly support a rotating element (11) to which the door (3) is rigidly attached. The flange (2) is equipped with an operating mechanism for opening and closing the door (3), comprising an operating rod (38) which passes through the outer face (8) of the flange (2) while being movable in translation, and a transmission (27) comprising at least one cam-groove linkage (CR2, CR3) for linking the rod (38) to the rotating element (11) in motion, so that a translation of the rod (38) causes a rotation of the door (3). Figure for the abstract: Figure 6

A cell transfer method includes supplying a culture medium to a bioreactor using a pipe in communication with the bioreactor, where cells remain in the pipe (first circulation flow path) to transfer cells remaining in the pipe to the bioreactor; acquiring a predetermined physical quantity related to the supply of the culture medium to the bioreactor; and stopping the supply of the culture medium to the bioreactor when the physical quantity reaches a predetermined threshold value.

The present invention provides a method, apparatus, system, and medium for processing cell preparations. The method involves performing a cell proliferation treatment on the target cell solution in a cell culture vessel to obtain a post-culture cell solution, then performing a washing and concentration treatment on the post-culture cell solution in the cell culture vessel to obtain a concentrated cell solution, and finally dispensing the concentrated cell solution from the cell culture vessel into multiple preparation bags. By performing cell culture, cell washing and concentration, and preparation dispensing all within the cell culture vessel, dispersed placement between devices is avoided, reducing occupied space. Executing the above steps sequentially within the cell culture vessel ensures that the entire process from cell culture to cell preparation is carried out in a single, sealed, sterile environment, eliminating the need for cell solution transfer and improving the quality of cell culture. By integrally executing the above steps sequentially within the cell culture vessel, manual intervention is reduced, the entire process from cell culture to cell preparation is intelligently executed, and the efficiency of cell culture is improved.

The utility model discloses automatic production equipment for cell drugs. The automatic production equipment comprises a process operation cavity and a cell factory incubator, the cell factory incubator is communicated with the process operation cavity; the process operation cavity is provided with a cell harvesting area and a cell inoculation liquid changing area; a centrifugal bottle pipetting and cover opening and closing assembly, a centrifugal assembly, a subpackaging assembly and a first transfer mechanism are arranged in the cell harvesting area; the first transfer mechanism is used for transferring the centrifugal bottle between the centrifugal bottle pipetting and cover opening and closing assembly and the centrifugal assembly; a cell factory pipetting and cover opening and closing assembly and a second transfer mechanism are arranged in the cell inoculation liquid changing area; the second transfer mechanism is used for transferring the cell factory among the cell factory pipetting and cover opening and closing assembly, the cell factory incubator and the centrifugal bottle pipetting and cover opening and closing assembly. By means of the arrangement, the function of full-process automation is achieved, and the purposes of reducing manual intervention and improving operation efficiency and sterility are achieved.

The present disclosure relates to a connection assembly for a tight transfer cell, comprising: – a cell flange (1) comprising an inner face equipped with a door (2) movable between a closed position for sealing a central opening (5) of the flange (1) and an open position for releasing this central opening (5), and an outer face (7) opposite the inner face; – a lock (13) borne by the cell flange (1) and located on its inner face (6) for locking the door (2) when it is closed on the opening (5); – an operating lever (17, 21) borne by the flange (1) being located on its outer face (7), for actuating the door (2) or for actuating the lock (13). According to the present disclosure, the flange (1) bears on its outer face (7) at least one magnetic indexing element (22, 23, 24, 27, 28) of the lever (17, 21) and the lever (17, 21) bears a magnetic element (26, 29).

1. The name of the design product: AGV transfer trolley. 2. The use of the design product: The design product is used for transferring cell incubators. 3. The design points of the design product: The overall shape of the product. 4. The picture or photo that best indicates the design points: perspective view 1. 5. The bottom view is not a common surface, and the bottom view is omitted. 6. Other circumstances that need to be explained: The use state diagram is the ascending state diagram of the design product.

The utility model discloses a multi-specification cell culture plate, relates to the technical field of cell culture plates, and aims to solve the problems that most of the conventional cell culture plates only have culture holes with single pore diameters, but the culture holes with the single pore diameters cause pollution when cells are transferred among different plates, and the whole culture effect is reduced. According to the technical scheme, the device is characterized by comprising a culture frame body, a first culture plate body, a second culture plate body and a third culture plate body which are distributed at equal intervals are detachably connected to the interior of the culture frame body, and a first culture groove, a second culture groove and a third culture groove are formed in the upper end of the first culture plate body, the upper end of the second culture plate body and the upper end of the third culture plate body respectively. The effects of reducing the pollution of the transferred cells and facilitating replacement, disassembly and assembly are achieved.

The utility model discloses a DC-CIK cell culture kit which comprises at least one DC bottle used for culturing DC cells; the at least one CIK bottle is detachably connected to the DC bottle and is used for culturing CIK cells; the at least one DC-CIK culture bottle is detachably connected to the DC bottle or the CIK bottle and is used for culturing DC-CIK cells; the culture bag is detachably connected to the DC-CIK culture bottle through a hose, and an opening and closing mechanism is arranged on the hose. According to the utility model, the whole-process culture of the DC-CIK cells can be completed on the premise that the cells are not transferred, the risk in the cell culture process is reduced, meanwhile, the technical requirements on producers can be reduced, and the cell culture efficiency is improved.

The utility model discloses an NK cell culture device which comprises a supporting frame, a first culture bottle, a second culture bottle and a culture bag are sequentially distributed on the supporting frame from top to bottom, an auxiliary support is arranged on the supporting frame, the auxiliary support is rotatably connected with the first culture bottle, a supporting seat is connected to the supporting frame in a sliding mode, and the supporting seat supports the bottom face of the first culture bottle. A liquid outlet is formed in the front side wall of the first culture bottle, a first hose is connected between the liquid outlet and a liquid inlet of the second culture bottle, and a second hose is connected between the second culture bottle and the culture bag. The cell transfer device is novel in structure, does not need to be taken out in the process of transferring cells twice, reduces the risk of pollution, is safe and reliable, reduces the use cost of relevant transfer consumables and instruments, and is easy for experimenters to master and operate.

Connecting assembly for a fluidtight transfer container with a fluidtight transfer cell including a cell flange and a cell door, the assembly including a container flange and a container door mounted in the container flange, wherein the container flange includes a first longitudinal connection portion to the cell and a second longitudinal connection portion to a container, the first longitudinal connection portion and the second longitudinal connection portion being connected by a shoulder opposite the container door.

The present disclosure relates generally to the field of genetic engineering of immune cells, in particular to mucosa-related constant T (MAIT) cells genetically engineered to express an exogenous T cell receptor (TCR) and uses thereof. More specifically, the present invention relates, in its embodiments, to cell compositions adapted for adoptive transfer cell therapy (ACT), providing improved therapeutic modalities.

Provided is an isolated culture method for primary umbilical cord mesenchymal stem cells. The method comprises the following steps: S1, pretreating umbilical cord tissue; S2, adding the pretreated umbilical cord tissue to a digestive fluid containing type II collagenase, and performing digestion via shaking to obtain a digested mixed solution; S3, diluting the digested mixed solution with normal saline, performing centrifugation to collect crude precipitates, re-suspending same with normal saline, and performing centrifugation and washing to obtain precipitates; S4, transferring the precipitates into a culture solution, and performing suspension culture in a carbon dioxide incubator; and S5, transferring cells which have been subjected to the suspension culture to a sterile centrifuge tube, pipetting and mixing uniformly, performing centrifugation and washing to collect cell precipitates, and performing adherent culture on the cell precipitates to obtain primary umbilical cord mesenchymal stem cells. By means of the method, highly viable and highly proliferative primary UCMSCs can be obtained within 4–8 days from a serum-free and serum-substitute-free culture solution with clear components, thereby establishing a foundation for downstream development of new drugs and clinical research.

The invention belongs to the technical field of medical treatment, and discloses a method and a system for separating self red blood cells. Comprising the following steps: washing red blood cells with normal saline for three times, removing supernatant as much as possible under the condition of retaining an albuginea layer, retaining packed red blood cells, and uniformly mixing; adding at least two tubes of uniformly mixed packed red cells into the capillary tube by using a sample adding gun; sealing the bottom of the pipe with sealing mud; putting the capillary tube with the sealing mud at one end downwards into a blood collection tube without an anticoagulant, and tightening a blood collection tube cap; centrifuging for 5 minutes by using a blood sample centrifugal machine, wherein the centrifugal force setting condition is 2000g or 3500rpm; after centrifugation, the sample is carefully and vertically taken out from the blood collection tube without shaking; cutting off a capillary tube at one end without sealing mud, namely a proximal end, and transferring cells 5 mm away from the upper part of the capillary tube into a 12 * 75 mm test tube; after washing with normal saline, centrifuging, introducing the intercepted 5mm capillary tube and washing liquid into a waste liquid cylinder, and retaining the volume of red blood cells. According to the method, the operation cost and the equipment investment are effectively reduced by reducing the equipment requirement and shortening the centrifugal time.

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

Disclosed herein is a microfluidic device for priming, collection and recovery of immune cells comprising: a) two separate fluidic channels: i) a first channel, wherein the first channel is infused with immune cells, and ii) a second channel, wherein the second channel acts as a collection reservoir for transmigrated cells, and; b) a microporous membrane interface, present between the first channel and the second channel, wherein the microporous membrane is functionalized on the first channel surface with adhesion molecules. Also disclosed are methods of creating enhanced cells, and using them in treatment of individuals in need thereof.

A device for efficiently transferring cells comprises a machine shell, a peristaltic pump is installed on one side in the machine shell, and a shaking table transmission mechanism is installed on the other side in the machine shell; a shaking table tray is mounted above the shaking table transmission mechanism; a semiconductor refrigeration module is mounted at the bottom in the shaking table tray; a display control module is installed above the peristaltic pump of the machine shell. A detachable battery pack is installed at the bottom of the machine shell. The display control module is respectively connected with the detachable battery pack, the semiconductor refrigeration module and the shaking table transmission mechanism; a cell culture container is arranged above the semiconductor refrigeration module; the cell culture container is connected with a liquid adding pipe; and the liquid adding pipe is connected with the peristaltic pump. According to the utility model, the refrigeration temperature can be adjusted through the display control module; in the transferring process, the shaking frequency can be adjusted through the display control module, and cell precipitation is effectively prevented; and the operation error is relatively small by adjusting the liquid flowing direction and the transfer volume through the peristaltic pump.

This invention relates to the field of single-cell functional analysis and microfluidic chip technology, and discloses a kit and method for in-situ sequential quantitative detection of secreted and intracellular proteins in the same single cell. The kit includes: a microcavity array chip; a secreted protein capture antibody barcode chip and an intracellular protein capture antibody barcode chip; a plastic clamp; a bioaffinity modification reagent; secreted protein standards, intracellular protein standards, secreted protein detection antibody stock solution, and intracellular protein detection antibody stock solution; a fluorescent conjugate, antibody buffer, antibody blocking solution, cell lysis buffer, and cell washing buffer. The method of this invention, through a sequential detection process of first capturing and incubating secreted proteins, followed by in-situ lysis to capture intracellular proteins, achieves high-throughput, high-sensitivity, and high-specificity in-situ sequential quantitative detection of secreted and intracellular proteins in the same single cell without cell transfer or loss of spatial location information. It can establish a correlation analysis of "intracellular signaling pathway activation - external functional output" in single cells, providing a powerful tool for disease mechanism research, drug screening, and immunotherapy evaluation.

A connection assembly for a sealed transfer cell, including a flange, a hinge, and a door connected to the flange by the hinge which can be moved between an open state and a closed state, the flange including central opening sealed by the door when this door is closed. The hinge includes a cylindrical rotary element securely connected to the door and two fixed bearings securely connected to the flange, the rotary element extending between the two bearings having two ends each engaged in a bearing, and this hinge contains an electric motor for opening and closing the door in a controlled manner.

The invention relates to systems comprising at least a cell culturing device and a cell sorting device, and optionally a direct connection from the cell culturing device to the cell sorting device, and a direct connection from the cell sorting device to the cell culturing device. The invention further relates to methods for developing and / or optimizing a cell culture process, comprising at least the steps of growing a cell culture of a polyclonal population of cells in a cell culturing device and selecting cells in the cell sorting device, and optionally transferring cells from the cell culturing device into a cell sorting device by direct connection(s) from the cell culturing device to the cell sorting device, and recirculating selected cells from the cell sorting device back to the cell culturing device by direct connection(s) from the cell sorting device to the cell culturing device.

A warehouse (10) for conditioning process of electrochemical cells (100), comprises a containment structure (12) inside which there are housed: at least one formation station (15) configured to receive at least one cell tray (11) containing electrochemical cells (100); at least one formation module (20), operative in the formation station (15), configured to be coupled to a cell tray (11) and to implement an at least partial formation cycle of electrochemical cells (100) contained in the cell tray (11); at least one aging station (16) configured to receive at least one cell tray (11) containing electrochemical cells (100); a transport system (19) to transfer cell trays (11) containing electrochemical cells (100) within the containment structure (12), wherein the transport system (19) is active at least between the formation station (15) and the aging station (16).

A roll stacking device for secondary batteries according to the present embodiment comprises: an input conveyor for transferring cells; a discharge conveyor spaced apart from the input conveyor; and a roll module having rolls for stacking the cells transported by the input conveyor on the upper side of the discharge conveyor in a forward-backward direction, wherein the rolls include a roll body on which at least one cell seating portion is formed and an adsorption pad which is mounted on the roll body and adsorbs the cells.

The subject matter of the present disclosure is a connection assembly for a tight transfer cell, comprising: - a cell flange (1) comprising a central opening (4), and an inner face (6) equipped with a door for sealing the opening (4); - a lock (13) borne by the inner face (6) of the cell flange (1) for locking the door; - a groove at the inner circumference of the opening for receiving outer lugs of a container flange in order to form a bayonet connection making it possible to couple this container flange; - a stop (31) movable in translation between an activation position wherein it protrudes into the groove to prevent uncoupling of the container flange, and a deactivation position wherein this movable stop releases the groove; - magnetic actuation means equipping the lock (13) and the movable stop (31) for deactivating the movable stop when this lock (13) switches to the locked state

This disclosure relates to a connection port (1) for a sealed transfer cell, this port comprising a flange (2) extending around a principal axis (AX), this flange (2) having an outer face (7) and an inner face (8) which is equipped with a door (3) movable between an open position and a closed position in which this door (3) closes a central opening (6) in the flange, and a slide (14) rigidly attached to the door (3) and passing through the flange (2), the slide (14) being connected to the flange (2) by a kinematic linkage with an axis parallel (AX1) to the principal axis (AX). The kinematic linkage comprises a sliding portion for restricting the slide (14) to a translational movement about the axis (AX1) during a first part of its movement, and a helical portion for restricting the slide (14) to a helical movement about the axis (AX1) during a second part of its movement. Figure for the abbreviation: Figure 2

To provide a holder with which cells can be observed in real time under a microscope and with which a support for cells after culturing can be readily collected from a culture medium and transferred.SOLUTION: A holder for holding a support used for cell culture, wherein the holder includes a plurality of recesses recessed from a planar surface toward a bottom surface in a plan view, a groove portion recessed from the planar surface toward the bottom surface in the plan view and provided so as to communicate with a part of each of the recesses, and one or more through-holes penetrating between the planar surface and the bottom surface, and each of the recesses has a side wall and includes an edge portion that extends in a direction away from the side wall on the bottom-surface side of the side wall and on which the support can be placed.SELECTED DRAWING: Figure 10