12 results about "Transfer cell" patented technology

A cell transfer apparatus transfers cells scattered in a first container to a second container. The cell transfer apparatus includes a head with a suction tip, a movement mechanism for the head, a camera that captures images of the first container, a cell recognition unit that performs position recognition of the cells based on the images, and a control unit that controls the head and the movement mechanism to perform a transfer operation of the cells based on a result of the position recognition. The control unit gradually suctions the set quantity of target cells that is set to two or more in advance into the suction tip from among the cells scattered in the first container, causes the head to move to the second container, and then discharges the set quantity of the target cells suctioned into the suction tip to the same location in the second container.

This disclosure relates to a connection port for a sealed transfer cell, this port comprising a flange (2) having an outer face (8) and an inner face (7) which carries a hinge (4) to which a door (3) movable between an open and a closed position is attached. The hinge (4) has two fixed bearings which jointly support a rotating element (11) to which the door (3) is rigidly attached. The flange (2) is equipped with an operating mechanism for opening and closing the door (3), comprising an operating rod (38) which passes through the outer face (8) of the flange (2) while being movable in translation, and a transmission (27) comprising at least one cam-groove linkage (CR2, CR3) for linking the rod (38) to the rotating element (11) in motion, so that a translation of the rod (38) causes a rotation of the door (3). Figure for the abstract: Figure 6

The utility model discloses automatic production equipment for cell drugs. The automatic production equipment comprises a process operation cavity and a cell factory incubator, the cell factory incubator is communicated with the process operation cavity; the process operation cavity is provided with a cell harvesting area and a cell inoculation liquid changing area; a centrifugal bottle pipetting and cover opening and closing assembly, a centrifugal assembly, a subpackaging assembly and a first transfer mechanism are arranged in the cell harvesting area; the first transfer mechanism is used for transferring the centrifugal bottle between the centrifugal bottle pipetting and cover opening and closing assembly and the centrifugal assembly; a cell factory pipetting and cover opening and closing assembly and a second transfer mechanism are arranged in the cell inoculation liquid changing area; the second transfer mechanism is used for transferring the cell factory among the cell factory pipetting and cover opening and closing assembly, the cell factory incubator and the centrifugal bottle pipetting and cover opening and closing assembly. By means of the arrangement, the function of full-process automation is achieved, and the purposes of reducing manual intervention and improving operation efficiency and sterility are achieved.

The utility model discloses a multi-specification cell culture plate, relates to the technical field of cell culture plates, and aims to solve the problems that most of the conventional cell culture plates only have culture holes with single pore diameters, but the culture holes with the single pore diameters cause pollution when cells are transferred among different plates, and the whole culture effect is reduced. According to the technical scheme, the device is characterized by comprising a culture frame body, a first culture plate body, a second culture plate body and a third culture plate body which are distributed at equal intervals are detachably connected to the interior of the culture frame body, and a first culture groove, a second culture groove and a third culture groove are formed in the upper end of the first culture plate body, the upper end of the second culture plate body and the upper end of the third culture plate body respectively. The effects of reducing the pollution of the transferred cells and facilitating replacement, disassembly and assembly are achieved.

The utility model discloses a DC-CIK cell culture kit which comprises at least one DC bottle used for culturing DC cells; the at least one CIK bottle is detachably connected to the DC bottle and is used for culturing CIK cells; the at least one DC-CIK culture bottle is detachably connected to the DC bottle or the CIK bottle and is used for culturing DC-CIK cells; the culture bag is detachably connected to the DC-CIK culture bottle through a hose, and an opening and closing mechanism is arranged on the hose. According to the utility model, the whole-process culture of the DC-CIK cells can be completed on the premise that the cells are not transferred, the risk in the cell culture process is reduced, meanwhile, the technical requirements on producers can be reduced, and the cell culture efficiency is improved.

The utility model discloses an NK cell culture device which comprises a supporting frame, a first culture bottle, a second culture bottle and a culture bag are sequentially distributed on the supporting frame from top to bottom, an auxiliary support is arranged on the supporting frame, the auxiliary support is rotatably connected with the first culture bottle, a supporting seat is connected to the supporting frame in a sliding mode, and the supporting seat supports the bottom face of the first culture bottle. A liquid outlet is formed in the front side wall of the first culture bottle, a first hose is connected between the liquid outlet and a liquid inlet of the second culture bottle, and a second hose is connected between the second culture bottle and the culture bag. The cell transfer device is novel in structure, does not need to be taken out in the process of transferring cells twice, reduces the risk of pollution, is safe and reliable, reduces the use cost of relevant transfer consumables and instruments, and is easy for experimenters to master and operate.

The present disclosure relates generally to the field of genetic engineering of immune cells, in particular to mucosa-related constant T (MAIT) cells genetically engineered to express an exogenous T cell receptor (TCR) and uses thereof. More specifically, the present invention relates, in its embodiments, to cell compositions adapted for adoptive transfer cell therapy (ACT), providing improved therapeutic modalities.

The invention belongs to the technical field of medical treatment, and discloses a method and a system for separating self red blood cells. Comprising the following steps: washing red blood cells with normal saline for three times, removing supernatant as much as possible under the condition of retaining an albuginea layer, retaining packed red blood cells, and uniformly mixing; adding at least two tubes of uniformly mixed packed red cells into the capillary tube by using a sample adding gun; sealing the bottom of the pipe with sealing mud; putting the capillary tube with the sealing mud at one end downwards into a blood collection tube without an anticoagulant, and tightening a blood collection tube cap; centrifuging for 5 minutes by using a blood sample centrifugal machine, wherein the centrifugal force setting condition is 2000g or 3500rpm; after centrifugation, the sample is carefully and vertically taken out from the blood collection tube without shaking; cutting off a capillary tube at one end without sealing mud, namely a proximal end, and transferring cells 5 mm away from the upper part of the capillary tube into a 12 * 75 mm test tube; after washing with normal saline, centrifuging, introducing the intercepted 5mm capillary tube and washing liquid into a waste liquid cylinder, and retaining the volume of red blood cells. According to the method, the operation cost and the equipment investment are effectively reduced by reducing the equipment requirement and shortening the centrifugal time.

This invention relates to the field of single-cell functional analysis and microfluidic chip technology, and discloses a kit and method for in-situ sequential quantitative detection of secreted and intracellular proteins in the same single cell. The kit includes: a microcavity array chip; a secreted protein capture antibody barcode chip and an intracellular protein capture antibody barcode chip; a plastic clamp; a bioaffinity modification reagent; secreted protein standards, intracellular protein standards, secreted protein detection antibody stock solution, and intracellular protein detection antibody stock solution; a fluorescent conjugate, antibody buffer, antibody blocking solution, cell lysis buffer, and cell washing buffer. The method of this invention, through a sequential detection process of first capturing and incubating secreted proteins, followed by in-situ lysis to capture intracellular proteins, achieves high-throughput, high-sensitivity, and high-specificity in-situ sequential quantitative detection of secreted and intracellular proteins in the same single cell without cell transfer or loss of spatial location information. It can establish a correlation analysis of "intracellular signaling pathway activation - external functional output" in single cells, providing a powerful tool for disease mechanism research, drug screening, and immunotherapy evaluation.

A connection assembly for a sealed transfer cell, including a flange, a hinge, and a door connected to the flange by the hinge which can be moved between an open state and a closed state, the flange including central opening sealed by the door when this door is closed. The hinge includes a cylindrical rotary element securely connected to the door and two fixed bearings securely connected to the flange, the rotary element extending between the two bearings having two ends each engaged in a bearing, and this hinge contains an electric motor for opening and closing the door in a controlled manner.

A roll stacking device for secondary batteries according to the present embodiment comprises: an input conveyor for transferring cells; a discharge conveyor spaced apart from the input conveyor; and a roll module having rolls for stacking the cells transported by the input conveyor on the upper side of the discharge conveyor in a forward-backward direction, wherein the rolls include a roll body on which at least one cell seating portion is formed and an adsorption pad which is mounted on the roll body and adsorbs the cells.

The subject matter of the present disclosure is a connection assembly for a tight transfer cell, comprising: - a cell flange (1) comprising a central opening (4), and an inner face (6) equipped with a door for sealing the opening (4); - a lock (13) borne by the inner face (6) of the cell flange (1) for locking the door; - a groove at the inner circumference of the opening for receiving outer lugs of a container flange in order to form a bayonet connection making it possible to couple this container flange; - a stop (31) movable in translation between an activation position wherein it protrudes into the groove to prevent uncoupling of the container flange, and a deactivation position wherein this movable stop releases the groove; - magnetic actuation means equipping the lock (13) and the movable stop (31) for deactivating the movable stop when this lock (13) switches to the locked state