58 results about "Transfer cell" patented technology

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

According to the cell electroporation method and device, an electroporation loading part formed by buckling a first electrode plate and a second electrode plate is arranged, and the number of electrode plates welded to each electrode plate is the same as the number of cell culture pore cavities of a standard cell porous plate; the adjacent first electrode plates and second electrode plates are combined to form a complete positive and negative electrode group, and the positions of the positive and negative electrode groups are in one-to-one correspondence with the cell culture pore cavities, so that a standard cell culture porous plate for bearing a cell suspension can be embedded into the electroporation loading part for direct electroporation without transferring the cell suspension; the non-contact butt joint between the standard cell culture porous plate and the electroporation loading part is realized, the damage of electroporation to cells is smaller, the cell survival rate is improved, a cell porous plate of a special specification does not need to be customized, plug-and-play is realized, the problem that an existing electroporation instrument is not matched with the standard cell culture porous plate is solved, the cost is reduced, the operation process is simplified, and the operation efficiency is improved. And the high-flux electrotransfection is realized.

A composite transfer cell and associated components and methods are presented. The composite transfer cell comprises a delivery system on a first side of the composite transfer cell; a tool on a second side of the composite transfer cell; and a composite transfer system between the first side and the second side of the composite transfer cell, the composite transfer system comprising a plurality of flipper arms independently movable in a vertical direction and rotatingly movable to lift and place a composite charge from the delivery system to the tool.

A cell transfer apparatus transfers cells scattered in a first container to a second container. The cell transfer apparatus includes a head with a suction tip, a movement mechanism for the head, a camera that captures images of the first container, a cell recognition unit that performs position recognition of the cells based on the images, and a control unit that controls the head and the movement mechanism to perform a transfer operation of the cells based on a result of the position recognition. The control unit gradually suctions the set quantity of target cells that is set to two or more in advance into the suction tip from among the cells scattered in the first container, causes the head to move to the second container, and then discharges the set quantity of the target cells suctioned into the suction tip to the same location in the second container.

Disclosed is a method for predicting the gene transfer rate in animal cell and / or the number of gene-transferred cells in the animal cells, the method comprising (1) a step for measuring a component in a supernatant of a culture obtained by the culturing of gene-transferred animal cells and (2) a step for predicting the gene transfer rate and / or the number of gene-transferred cells on the basis of a value measured in step (1).

The present disclosure relates to a connection port (1) for a tight transfer cell, this port comprising a flange (2) extending about a main axis (AX), this flange (2) comprising an outer face (7) and an inner face (8) which is equipped with a door (3) movable between an open position and a closed position wherein this door (3) seals a central opening (6) of the flange, and a slide (14) rigidly secured to the door (3) and passing through the flange (2), the slide (14) being connected to the flange (2) by a kinematic connection with a parallel axis (AX1) to the main axis (AX). The kinematic connection comprises a slide connection portion for applying a translational movement to the slide (14) along the axis (AX1) on a first displacement part, and a helical connection portion for applying a helical movement to the slide (14) about the axis (AX1) on a second displacement portion.

The invention discloses a liquid path system and an automatic needle changing structure for micromanipulation, and relates to the technical field of micromanipulation. The liquid suction and spitting structure aims to solve the problem that gas in an air cavity of an existing liquid suction and spitting structure is prone to being compressed, and the deviation between liquid relief precision and expectation is too large. The liquid path system comprises a liquid driving unit, the liquid driving unit is detachably connected with a liquid inlet of a micromanipulation needle of a micromanipulation automatic needle changing unit, and incompressible liquid is injected into the micromanipulation needle, so that a continuous liquid column without an air cavity is formed between the liquid driving unit and the micromanipulation needle; liquid is used as a medium to suck and spit cells or tissue fragments, and perfusion or cleaning is carried out; the automatic needle changing structure comprises a liquid path system and a micromanipulation automatic needle changing unit, and the liquid path system is connected with the micromanipulation automatic needle changing structure and used for transferring cells or tissue fragments with liquid as a medium in micromanipulation. The method is used for cell culture and organoid culture.

The present disclosure relates to a connection port for a sealed transfer cell, this port including a flange (2) having an outer face (8) and an inner face (7) that carries a hinge (4) to which a door (3) movable between an open position and a closed position is attached, the hinge (4) including two fixed bearings that jointly carry a rotary element (11) 5 to which the door (3) is rigidly attached. The flange (2) is equipped with a manoeuvring system for opening and closing the door (3), including a manoeuvring rod (38) that passes through the outer face (8) of the flange (2) while being movable in translation, and a transmission (27) including at least one cam-groove connection (CR2, CR3) to movably link the rod (38) to the rotary element (11), so that a translation of the rod (38) causes a rotation 10 of the door (3).

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

This disclosure relates to a connection port for a sealed transfer cell, this port comprising a flange (2) having an outer face (8) and an inner face (7) which carries a hinge (4) to which a door (3) movable between an open and a closed position is attached. The hinge (4) has two fixed bearings which jointly support a rotating element (11) to which the door (3) is rigidly attached. The flange (2) is equipped with an operating mechanism for opening and closing the door (3), comprising an operating rod (38) which passes through the outer face (8) of the flange (2) while being movable in translation, and a transmission (27) comprising at least one cam-groove linkage (CR2, CR3) for linking the rod (38) to the rotating element (11) in motion, so that a translation of the rod (38) causes a rotation of the door (3). Figure for the abstract: Figure 6

The invention belongs to the technical field of cell culture, and discloses a low-frequency pulse electromagnetic field carbon dioxide constant-temperature cell culture box which comprises a culture box body, a Helmholtz coil is connected in the culture box body, and the culture box body is connected with a supporting assembly; the supporting assembly is slidably connected with a storage plate. The storage plate is located between two coils of the Helmholtz coil, and the storage plate is used for placing a culture dish; a low-frequency pulse electromagnetic field generator and an oscilloscope are integrated on the upper portion of the incubator body, and the Helmholtz coil is connected to a control panel on the upper portion of the incubator body through an internal wire. The problems that an existing cell culture box is easily infected by bacteria and fungi due to repeated cell transfer, the time for irradiating a cell culture dish by an electromagnetic field cannot be accurately controlled, and a large amount of manpower and material resources are consumed due to repeated parameter modification, cell culture dish movement and cable connection are solved.

A cell transfer method includes supplying a culture medium to a bioreactor using a pipe in communication with the bioreactor, where cells remain in the pipe (first circulation flow path) to transfer cells remaining in the pipe to the bioreactor; acquiring a predetermined physical quantity related to the supply of the culture medium to the bioreactor; and stopping the supply of the culture medium to the bioreactor when the physical quantity reaches a predetermined threshold value.

The present invention provides a method, apparatus, system, and medium for processing cell preparations. The method involves performing a cell proliferation treatment on the target cell solution in a cell culture vessel to obtain a post-culture cell solution, then performing a washing and concentration treatment on the post-culture cell solution in the cell culture vessel to obtain a concentrated cell solution, and finally dispensing the concentrated cell solution from the cell culture vessel into multiple preparation bags. By performing cell culture, cell washing and concentration, and preparation dispensing all within the cell culture vessel, dispersed placement between devices is avoided, reducing occupied space. Executing the above steps sequentially within the cell culture vessel ensures that the entire process from cell culture to cell preparation is carried out in a single, sealed, sterile environment, eliminating the need for cell solution transfer and improving the quality of cell culture. By integrally executing the above steps sequentially within the cell culture vessel, manual intervention is reduced, the entire process from cell culture to cell preparation is intelligently executed, and the efficiency of cell culture is improved.

The invention relates to the technical field of cell biomechanics, and discloses a test device for applying periodic pressure to cells, the test device comprises a bottom plate, a slide rail assembly is arranged above the bottom plate, a multi-cavity culture dish is placed at the right end of the slide rail assembly, and a driving assembly and a driven assembly are fixedly mounted at the top end of the bottom plate; a dripping assembly is arranged above the multi-cavity culture dish. A multi-cavity culture dish is pushed to move leftwards in a sliding rail assembly when a pushing piece moves, when the multi-cavity culture dish makes contact with a lifting frame, the lifting frame can be pressed downwards, then a first rack is pushed upwards, the first rack pushes a first gear to rotate anticlockwise, a rotating shaft and an extrusion shaft are driven to rotate, and then a hose part of a feeding pipe is extruded; according to the multi-cavity culture dish, the cells in the feeding pipe are squeezed into the multi-cavity culture dish, automatic cell transfer is achieved, the cells can be dripped into each cavity in the multi-cavity culture dish by arranging the three sets of lifting frames, the experiment efficiency is improved, and manpower is reduced.

The utility model discloses automatic production equipment for cell drugs. The automatic production equipment comprises a process operation cavity and a cell factory incubator, the cell factory incubator is communicated with the process operation cavity; the process operation cavity is provided with a cell harvesting area and a cell inoculation liquid changing area; a centrifugal bottle pipetting and cover opening and closing assembly, a centrifugal assembly, a subpackaging assembly and a first transfer mechanism are arranged in the cell harvesting area; the first transfer mechanism is used for transferring the centrifugal bottle between the centrifugal bottle pipetting and cover opening and closing assembly and the centrifugal assembly; a cell factory pipetting and cover opening and closing assembly and a second transfer mechanism are arranged in the cell inoculation liquid changing area; the second transfer mechanism is used for transferring the cell factory among the cell factory pipetting and cover opening and closing assembly, the cell factory incubator and the centrifugal bottle pipetting and cover opening and closing assembly. By means of the arrangement, the function of full-process automation is achieved, and the purposes of reducing manual intervention and improving operation efficiency and sterility are achieved.

The present disclosure relates to a connection assembly for a tight transfer cell, comprising: – a cell flange (1) comprising an inner face equipped with a door (2) movable between a closed position for sealing a central opening (5) of the flange (1) and an open position for releasing this central opening (5), and an outer face (7) opposite the inner face; – a lock (13) borne by the cell flange (1) and located on its inner face (6) for locking the door (2) when it is closed on the opening (5); – an operating lever (17, 21) borne by the flange (1) being located on its outer face (7), for actuating the door (2) or for actuating the lock (13). According to the present disclosure, the flange (1) bears on its outer face (7) at least one magnetic indexing element (22, 23, 24, 27, 28) of the lever (17, 21) and the lever (17, 21) bears a magnetic element (26, 29).

1. The name of the design product: AGV transfer trolley. 2. The use of the design product: The design product is used for transferring cell incubators. 3. The design points of the design product: The overall shape of the product. 4. The picture or photo that best indicates the design points: perspective view 1. 5. The bottom view is not a common surface, and the bottom view is omitted. 6. Other circumstances that need to be explained: The use state diagram is the ascending state diagram of the design product.

Provided is a fully automatic suspension cell culture bag kit, comprising a primary cell storage bag, an initial cell activation bag, a liquid waste bag and a cell culture bag, wherein the bags are connected to each other by means of T-joints and a plurality of pipes. The cell culture bag kit eliminates the need for the manual liquid transfer operation of transferring cells into the bags from a bottle during a suspension cell culture process, such that the risk of bacterial contamination during the cell culture process is significantly reduced.

A connection assembly for a sealed transfer cell, including a flange, a hinge, and a door connected to the flange by the hinge which can be moved between an open state and a closed state, the flange including central opening sealed by the door when this door is closed. The hinge includes a cylindrical rotary element securely connected to the door and two fixed bearings securely connected to the flange, the rotary element extending between the two bearings having two ends each engaged in a bearing, and this hinge contains an electric motor for opening and closing the door in a controlled manner.

The invention relates to the technical field of microinjection, and discloses a self-adaptive dynamic adjustment automatic microinjection method, which comprises the step of transferring cells with mark point arrays formed on the surfaces in a culture dish containing marking substances. The temperature, humidity and vibration parameters of the environment where the cells are located are collected in real time through multiple sensors. The cells are irradiated with light sources of different wavelengths, and images are captured. Acquiring position information of cell surface mark points, and establishing and optimizing a cell three-dimensional dynamic model. And determining an injection target, planning a path and generating a control instruction. The flexible injection needle is controlled to move and inject substances, the cell state and environmental parameters are monitored in real time during injection, the upper computer adjusts related parameters according to a self-adaptive control algorithm, and the needle is withdrawn to the initial position after injection. The micro-injection precision and stability can be effectively improved, cell damage is reduced, the experiment success rate is increased, and powerful support is provided for cell research and biotechnology application.

The utility model discloses a multi-specification cell culture plate, relates to the technical field of cell culture plates, and aims to solve the problems that most of the conventional cell culture plates only have culture holes with single pore diameters, but the culture holes with the single pore diameters cause pollution when cells are transferred among different plates, and the whole culture effect is reduced. According to the technical scheme, the device is characterized by comprising a culture frame body, a first culture plate body, a second culture plate body and a third culture plate body which are distributed at equal intervals are detachably connected to the interior of the culture frame body, and a first culture groove, a second culture groove and a third culture groove are formed in the upper end of the first culture plate body, the upper end of the second culture plate body and the upper end of the third culture plate body respectively. The effects of reducing the pollution of the transferred cells and facilitating replacement, disassembly and assembly are achieved.

The utility model discloses a DC-CIK cell culture kit which comprises at least one DC bottle used for culturing DC cells; the at least one CIK bottle is detachably connected to the DC bottle and is used for culturing CIK cells; the at least one DC-CIK culture bottle is detachably connected to the DC bottle or the CIK bottle and is used for culturing DC-CIK cells; the culture bag is detachably connected to the DC-CIK culture bottle through a hose, and an opening and closing mechanism is arranged on the hose. According to the utility model, the whole-process culture of the DC-CIK cells can be completed on the premise that the cells are not transferred, the risk in the cell culture process is reduced, meanwhile, the technical requirements on producers can be reduced, and the cell culture efficiency is improved.

The utility model discloses an NK cell culture device which comprises a supporting frame, a first culture bottle, a second culture bottle and a culture bag are sequentially distributed on the supporting frame from top to bottom, an auxiliary support is arranged on the supporting frame, the auxiliary support is rotatably connected with the first culture bottle, a supporting seat is connected to the supporting frame in a sliding mode, and the supporting seat supports the bottom face of the first culture bottle. A liquid outlet is formed in the front side wall of the first culture bottle, a first hose is connected between the liquid outlet and a liquid inlet of the second culture bottle, and a second hose is connected between the second culture bottle and the culture bag. The cell transfer device is novel in structure, does not need to be taken out in the process of transferring cells twice, reduces the risk of pollution, is safe and reliable, reduces the use cost of relevant transfer consumables and instruments, and is easy for experimenters to master and operate.

Connecting assembly for a fluidtight transfer container with a fluidtight transfer cell including a cell flange and a cell door, the assembly including a container flange and a container door mounted in the container flange, wherein the container flange includes a first longitudinal connection portion to the cell and a second longitudinal connection portion to a container, the first longitudinal connection portion and the second longitudinal connection portion being connected by a shoulder opposite the container door.

The present invention discloses a cell storage and transplantation device for small animals under the renal capsule, and relates to the field of experimental instruments for islet transplantation. The cell storage and transplantation device for small animals under the renal capsule includes two major parts: a storage tube and a storage box. The storage tube includes an outer tube and an inner tube. The inner side of the storage box ice box is provided with six placement slots. The device provided by the present invention can directly store cell suspensions. The storage tube can be directly placed in a low-speed centrifuge for centrifugation. The cells will settle to the lower section of the inner tube of the storage tube. The storage tube with centrifuged cells can be directly placed in a matching storage box for storage and transportation. The cells do not need to be transferred before cell transplantation, and the cell viability is greatly improved. The inner tube of the storage tube is compatible with the size of a 10μL pipette, and the transplantation can be directly performed with a 10μL pipette. When injecting, the round closed end of the gel at the end of the lower section of the inner tube of the storage tube is peeled off, and the cell transplantation can be performed directly.

The present disclosure relates generally to the field of genetic engineering of immune cells, in particular to mucosa-related constant T (MAIT) cells genetically engineered to express an exogenous T cell receptor (TCR) and uses thereof. More specifically, the present invention relates, in its embodiments, to cell compositions adapted for adoptive transfer cell therapy (ACT), providing improved therapeutic modalities.

The utility model relates to the technical field of medical instruments, and discloses a pair of cell cryopreservation resuscitation transfer forceps, which comprises a first forceps rod and a second forceps rod, arc-shaped jaws are respectively arranged on the opposite sides of the lower ends of the first forceps rod and the second forceps rod, and a plurality of groups of tooth-shaped bulges are arranged on the inner sides of the arc-shaped jaws; a braking part is installed on the upper portion of the first forceps rod, multiple first braking racks are arranged on the side, close to the second forceps rod, of the braking part, a groove body matched with the braking part to be inserted is formed in the upper portion of the second forceps rod, a movable plate is arranged in the groove body, and multiple second braking racks are installed on the side, close to the first forceps rod, of the movable plate. Compared with the prior art, the cell cryopreservation tube clamp has the advantages that when cells are cryopreserved or resuscitated and transferred, the cell cryopreservation tube is more effectively clamped by the arc-shaped jaw, so that the cell cryopreservation tube is not easy to fall off, and the pollution and death rate of the cells are reduced. Due to the design of the brake part, when the cryopreservation tube is clamped, the force generated by the hand is saved, and the fatigue and discomfort of the hand are reduced.

The object of the present disclosure is a connection assembly for a sealed transfer cell, including: – a cell flange (1) including a central opening (4), and an inner face (6) equipped with a door to close the opening (4); – a lock (14) carried by the inner face (6) of the cell flange (1) to lock the door; – a groove at the inner circumference of the opening for receiving outer lugs of a container flange in order to form a bayonet connection making it possible to couple this container flange; – a stop (31) rotatably movable between an activation position wherein it protrudes into the groove to prohibit the uncoupling of the container flange, and a deactivation position wherein this movable stop releases the groove; – actuating means equipping the lock (14) to deactivate the movable stop when this lock (14) changes to the locked state.

A cell transfer apparatus transfers cells scattered in a first container to a second container. The cell transfer apparatus includes a head to which a suction tip that can generate suction force and discharge force in a distal end opening is attached, a movement mechanism for the head, a camera that captures images of the first container, a cell recognition unit that performs position recognition of the cells based on the images, and a control unit that controls the head and the movement mechanism to perform a transfer operation of the cells based on a result of the position recognition. The control unit gradually suctions the set quantity of target cells that is set to two or more in advance into the suction tip from among the cells scattered in the first container, causes the head to move to the second container, and then discharges the set quantity of the target cells that are suctioned into the suction tip to the same location in the second container.

The invention relates to the technical field of cell culture, in particular to an enhanced CIK cell as well as a preparation method and application thereof. The preparation method of the enhanced CIK cells comprises the following steps: (1) mixing mononuclear cells with a serum-free culture medium, and culturing until the cell density is (2-6) * 10 < 6 > cells / mL; (2) transferring the cell suspension to a special culture medium for CIK cells, culturing for 5-7 days, adding a taxus chinensis extract and ginsenoside, and acting on the cells for 2-3 days; and (3) collecting the cells to obtain the enhanced CIK cells. The enhanced CIK cell prepared by using the method disclosed by the invention is high in proliferation speed and high in tumor killing capability, and a basis is provided for development of tumor drugs.