45 results about "Transfer cell" patented technology

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

A composite transfer cell and associated components and methods are presented. The composite transfer cell comprises a delivery system on a first side of the composite transfer cell; a tool on a second side of the composite transfer cell; and a composite transfer system between the first side and the second side of the composite transfer cell, the composite transfer system comprising a plurality of flipper arms independently movable in a vertical direction and rotatingly movable to lift and place a composite charge from the delivery system to the tool.

A cell transfer apparatus transfers cells scattered in a first container to a second container. The cell transfer apparatus includes a head with a suction tip, a movement mechanism for the head, a camera that captures images of the first container, a cell recognition unit that performs position recognition of the cells based on the images, and a control unit that controls the head and the movement mechanism to perform a transfer operation of the cells based on a result of the position recognition. The control unit gradually suctions the set quantity of target cells that is set to two or more in advance into the suction tip from among the cells scattered in the first container, causes the head to move to the second container, and then discharges the set quantity of the target cells suctioned into the suction tip to the same location in the second container.

The present disclosure relates to a connection port (1) for a tight transfer cell, this port comprising a flange (2) extending about a main axis (AX), this flange (2) comprising an outer face (7) and an inner face (8) which is equipped with a door (3) movable between an open position and a closed position wherein this door (3) seals a central opening (6) of the flange, and a slide (14) rigidly secured to the door (3) and passing through the flange (2), the slide (14) being connected to the flange (2) by a kinematic connection with a parallel axis (AX1) to the main axis (AX). The kinematic connection comprises a slide connection portion for applying a translational movement to the slide (14) along the axis (AX1) on a first displacement part, and a helical connection portion for applying a helical movement to the slide (14) about the axis (AX1) on a second displacement portion.

The invention discloses a liquid path system and an automatic needle changing structure for micromanipulation, and relates to the technical field of micromanipulation. The liquid suction and spitting structure aims to solve the problem that gas in an air cavity of an existing liquid suction and spitting structure is prone to being compressed, and the deviation between liquid relief precision and expectation is too large. The liquid path system comprises a liquid driving unit, the liquid driving unit is detachably connected with a liquid inlet of a micromanipulation needle of a micromanipulation automatic needle changing unit, and incompressible liquid is injected into the micromanipulation needle, so that a continuous liquid column without an air cavity is formed between the liquid driving unit and the micromanipulation needle; liquid is used as a medium to suck and spit cells or tissue fragments, and perfusion or cleaning is carried out; the automatic needle changing structure comprises a liquid path system and a micromanipulation automatic needle changing unit, and the liquid path system is connected with the micromanipulation automatic needle changing structure and used for transferring cells or tissue fragments with liquid as a medium in micromanipulation. The method is used for cell culture and organoid culture.

The present disclosure relates to a connection port for a sealed transfer cell, this port including a flange (2) having an outer face (8) and an inner face (7) that carries a hinge (4) to which a door (3) movable between an open position and a closed position is attached, the hinge (4) including two fixed bearings that jointly carry a rotary element (11) 5 to which the door (3) is rigidly attached. The flange (2) is equipped with a manoeuvring system for opening and closing the door (3), including a manoeuvring rod (38) that passes through the outer face (8) of the flange (2) while being movable in translation, and a transmission (27) including at least one cam-groove connection (CR2, CR3) to movably link the rod (38) to the rotary element (11), so that a translation of the rod (38) causes a rotation 10 of the door (3).

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

This disclosure relates to a connection port for a sealed transfer cell, this port comprising a flange (2) having an outer face (8) and an inner face (7) which carries a hinge (4) to which a door (3) movable between an open and a closed position is attached. The hinge (4) has two fixed bearings which jointly support a rotating element (11) to which the door (3) is rigidly attached. The flange (2) is equipped with an operating mechanism for opening and closing the door (3), comprising an operating rod (38) which passes through the outer face (8) of the flange (2) while being movable in translation, and a transmission (27) comprising at least one cam-groove linkage (CR2, CR3) for linking the rod (38) to the rotating element (11) in motion, so that a translation of the rod (38) causes a rotation of the door (3). Figure for the abstract: Figure 6

The invention belongs to the technical field of cell culture, and discloses a low-frequency pulse electromagnetic field carbon dioxide constant-temperature cell culture box which comprises a culture box body, a Helmholtz coil is connected in the culture box body, and the culture box body is connected with a supporting assembly; the supporting assembly is slidably connected with a storage plate. The storage plate is located between two coils of the Helmholtz coil, and the storage plate is used for placing a culture dish; a low-frequency pulse electromagnetic field generator and an oscilloscope are integrated on the upper portion of the incubator body, and the Helmholtz coil is connected to a control panel on the upper portion of the incubator body through an internal wire. The problems that an existing cell culture box is easily infected by bacteria and fungi due to repeated cell transfer, the time for irradiating a cell culture dish by an electromagnetic field cannot be accurately controlled, and a large amount of manpower and material resources are consumed due to repeated parameter modification, cell culture dish movement and cable connection are solved.

A cell transfer method includes supplying a culture medium to a bioreactor using a pipe in communication with the bioreactor, where cells remain in the pipe (first circulation flow path) to transfer cells remaining in the pipe to the bioreactor; acquiring a predetermined physical quantity related to the supply of the culture medium to the bioreactor; and stopping the supply of the culture medium to the bioreactor when the physical quantity reaches a predetermined threshold value.

The present invention provides a method, apparatus, system, and medium for processing cell preparations. The method involves performing a cell proliferation treatment on the target cell solution in a cell culture vessel to obtain a post-culture cell solution, then performing a washing and concentration treatment on the post-culture cell solution in the cell culture vessel to obtain a concentrated cell solution, and finally dispensing the concentrated cell solution from the cell culture vessel into multiple preparation bags. By performing cell culture, cell washing and concentration, and preparation dispensing all within the cell culture vessel, dispersed placement between devices is avoided, reducing occupied space. Executing the above steps sequentially within the cell culture vessel ensures that the entire process from cell culture to cell preparation is carried out in a single, sealed, sterile environment, eliminating the need for cell solution transfer and improving the quality of cell culture. By integrally executing the above steps sequentially within the cell culture vessel, manual intervention is reduced, the entire process from cell culture to cell preparation is intelligently executed, and the efficiency of cell culture is improved.

The invention relates to the technical field of cell biomechanics, and discloses a test device for applying periodic pressure to cells, the test device comprises a bottom plate, a slide rail assembly is arranged above the bottom plate, a multi-cavity culture dish is placed at the right end of the slide rail assembly, and a driving assembly and a driven assembly are fixedly mounted at the top end of the bottom plate; a dripping assembly is arranged above the multi-cavity culture dish. A multi-cavity culture dish is pushed to move leftwards in a sliding rail assembly when a pushing piece moves, when the multi-cavity culture dish makes contact with a lifting frame, the lifting frame can be pressed downwards, then a first rack is pushed upwards, the first rack pushes a first gear to rotate anticlockwise, a rotating shaft and an extrusion shaft are driven to rotate, and then a hose part of a feeding pipe is extruded; according to the multi-cavity culture dish, the cells in the feeding pipe are squeezed into the multi-cavity culture dish, automatic cell transfer is achieved, the cells can be dripped into each cavity in the multi-cavity culture dish by arranging the three sets of lifting frames, the experiment efficiency is improved, and manpower is reduced.

The utility model discloses automatic production equipment for cell drugs. The automatic production equipment comprises a process operation cavity and a cell factory incubator, the cell factory incubator is communicated with the process operation cavity; the process operation cavity is provided with a cell harvesting area and a cell inoculation liquid changing area; a centrifugal bottle pipetting and cover opening and closing assembly, a centrifugal assembly, a subpackaging assembly and a first transfer mechanism are arranged in the cell harvesting area; the first transfer mechanism is used for transferring the centrifugal bottle between the centrifugal bottle pipetting and cover opening and closing assembly and the centrifugal assembly; a cell factory pipetting and cover opening and closing assembly and a second transfer mechanism are arranged in the cell inoculation liquid changing area; the second transfer mechanism is used for transferring the cell factory among the cell factory pipetting and cover opening and closing assembly, the cell factory incubator and the centrifugal bottle pipetting and cover opening and closing assembly. By means of the arrangement, the function of full-process automation is achieved, and the purposes of reducing manual intervention and improving operation efficiency and sterility are achieved.

The present disclosure relates to a connection assembly for a tight transfer cell, comprising: – a cell flange (1) comprising an inner face equipped with a door (2) movable between a closed position for sealing a central opening (5) of the flange (1) and an open position for releasing this central opening (5), and an outer face (7) opposite the inner face; – a lock (13) borne by the cell flange (1) and located on its inner face (6) for locking the door (2) when it is closed on the opening (5); – an operating lever (17, 21) borne by the flange (1) being located on its outer face (7), for actuating the door (2) or for actuating the lock (13). According to the present disclosure, the flange (1) bears on its outer face (7) at least one magnetic indexing element (22, 23, 24, 27, 28) of the lever (17, 21) and the lever (17, 21) bears a magnetic element (26, 29).

1. The name of the design product: AGV transfer trolley. 2. The use of the design product: The design product is used for transferring cell incubators. 3. The design points of the design product: The overall shape of the product. 4. The picture or photo that best indicates the design points: perspective view 1. 5. The bottom view is not a common surface, and the bottom view is omitted. 6. Other circumstances that need to be explained: The use state diagram is the ascending state diagram of the design product.

The invention relates to the technical field of microinjection, and discloses a self-adaptive dynamic adjustment automatic microinjection method, which comprises the step of transferring cells with mark point arrays formed on the surfaces in a culture dish containing marking substances. The temperature, humidity and vibration parameters of the environment where the cells are located are collected in real time through multiple sensors. The cells are irradiated with light sources of different wavelengths, and images are captured. Acquiring position information of cell surface mark points, and establishing and optimizing a cell three-dimensional dynamic model. And determining an injection target, planning a path and generating a control instruction. The flexible injection needle is controlled to move and inject substances, the cell state and environmental parameters are monitored in real time during injection, the upper computer adjusts related parameters according to a self-adaptive control algorithm, and the needle is withdrawn to the initial position after injection. The micro-injection precision and stability can be effectively improved, cell damage is reduced, the experiment success rate is increased, and powerful support is provided for cell research and biotechnology application.

The utility model discloses a multi-specification cell culture plate, relates to the technical field of cell culture plates, and aims to solve the problems that most of the conventional cell culture plates only have culture holes with single pore diameters, but the culture holes with the single pore diameters cause pollution when cells are transferred among different plates, and the whole culture effect is reduced. According to the technical scheme, the device is characterized by comprising a culture frame body, a first culture plate body, a second culture plate body and a third culture plate body which are distributed at equal intervals are detachably connected to the interior of the culture frame body, and a first culture groove, a second culture groove and a third culture groove are formed in the upper end of the first culture plate body, the upper end of the second culture plate body and the upper end of the third culture plate body respectively. The effects of reducing the pollution of the transferred cells and facilitating replacement, disassembly and assembly are achieved.

The utility model discloses a DC-CIK cell culture kit which comprises at least one DC bottle used for culturing DC cells; the at least one CIK bottle is detachably connected to the DC bottle and is used for culturing CIK cells; the at least one DC-CIK culture bottle is detachably connected to the DC bottle or the CIK bottle and is used for culturing DC-CIK cells; the culture bag is detachably connected to the DC-CIK culture bottle through a hose, and an opening and closing mechanism is arranged on the hose. According to the utility model, the whole-process culture of the DC-CIK cells can be completed on the premise that the cells are not transferred, the risk in the cell culture process is reduced, meanwhile, the technical requirements on producers can be reduced, and the cell culture efficiency is improved.

The utility model discloses an NK cell culture device which comprises a supporting frame, a first culture bottle, a second culture bottle and a culture bag are sequentially distributed on the supporting frame from top to bottom, an auxiliary support is arranged on the supporting frame, the auxiliary support is rotatably connected with the first culture bottle, a supporting seat is connected to the supporting frame in a sliding mode, and the supporting seat supports the bottom face of the first culture bottle. A liquid outlet is formed in the front side wall of the first culture bottle, a first hose is connected between the liquid outlet and a liquid inlet of the second culture bottle, and a second hose is connected between the second culture bottle and the culture bag. The cell transfer device is novel in structure, does not need to be taken out in the process of transferring cells twice, reduces the risk of pollution, is safe and reliable, reduces the use cost of relevant transfer consumables and instruments, and is easy for experimenters to master and operate.

Connecting assembly for a fluidtight transfer container with a fluidtight transfer cell including a cell flange and a cell door, the assembly including a container flange and a container door mounted in the container flange, wherein the container flange includes a first longitudinal connection portion to the cell and a second longitudinal connection portion to a container, the first longitudinal connection portion and the second longitudinal connection portion being connected by a shoulder opposite the container door.

The present disclosure relates generally to the field of genetic engineering of immune cells, in particular to mucosa-related constant T (MAIT) cells genetically engineered to express an exogenous T cell receptor (TCR) and uses thereof. More specifically, the present invention relates, in its embodiments, to cell compositions adapted for adoptive transfer cell therapy (ACT), providing improved therapeutic modalities.

The utility model relates to the technical field of medical instruments, and discloses a pair of cell cryopreservation resuscitation transfer forceps, which comprises a first forceps rod and a second forceps rod, arc-shaped jaws are respectively arranged on the opposite sides of the lower ends of the first forceps rod and the second forceps rod, and a plurality of groups of tooth-shaped bulges are arranged on the inner sides of the arc-shaped jaws; a braking part is installed on the upper portion of the first forceps rod, multiple first braking racks are arranged on the side, close to the second forceps rod, of the braking part, a groove body matched with the braking part to be inserted is formed in the upper portion of the second forceps rod, a movable plate is arranged in the groove body, and multiple second braking racks are installed on the side, close to the first forceps rod, of the movable plate. Compared with the prior art, the cell cryopreservation tube clamp has the advantages that when cells are cryopreserved or resuscitated and transferred, the cell cryopreservation tube is more effectively clamped by the arc-shaped jaw, so that the cell cryopreservation tube is not easy to fall off, and the pollution and death rate of the cells are reduced. Due to the design of the brake part, when the cryopreservation tube is clamped, the force generated by the hand is saved, and the fatigue and discomfort of the hand are reduced.

The object of the present disclosure is a connection assembly for a sealed transfer cell, including: – a cell flange (1) including a central opening (4), and an inner face (6) equipped with a door to close the opening (4); – a lock (14) carried by the inner face (6) of the cell flange (1) to lock the door; – a groove at the inner circumference of the opening for receiving outer lugs of a container flange in order to form a bayonet connection making it possible to couple this container flange; – a stop (31) rotatably movable between an activation position wherein it protrudes into the groove to prohibit the uncoupling of the container flange, and a deactivation position wherein this movable stop releases the groove; – actuating means equipping the lock (14) to deactivate the movable stop when this lock (14) changes to the locked state.

Provided is an isolated culture method for primary umbilical cord mesenchymal stem cells. The method comprises the following steps: S1, pretreating umbilical cord tissue; S2, adding the pretreated umbilical cord tissue to a digestive fluid containing type II collagenase, and performing digestion via shaking to obtain a digested mixed solution; S3, diluting the digested mixed solution with normal saline, performing centrifugation to collect crude precipitates, re-suspending same with normal saline, and performing centrifugation and washing to obtain precipitates; S4, transferring the precipitates into a culture solution, and performing suspension culture in a carbon dioxide incubator; and S5, transferring cells which have been subjected to the suspension culture to a sterile centrifuge tube, pipetting and mixing uniformly, performing centrifugation and washing to collect cell precipitates, and performing adherent culture on the cell precipitates to obtain primary umbilical cord mesenchymal stem cells. By means of the method, highly viable and highly proliferative primary UCMSCs can be obtained within 4–8 days from a serum-free and serum-substitute-free culture solution with clear components, thereby establishing a foundation for downstream development of new drugs and clinical research.

The utility model discloses a suction tube convenient for cell harvesting and passage, which relates to the technical field of cell passage scraping, and adopts the technical scheme that the suction tube comprises a suction head, a conical tube and a thick-diameter tube, the top and the bottom of the thick-diameter tube are respectively connected with the suction head and the conical tube, and the suction tube is characterized in that a rotating device is arranged at the bottom of the conical tube; a suction pipe is arranged at the end, away from the conical pipe, of the rotating device; the rotating device comprises a spherical shell and a rotating spherical shell; the top of the spherical shell is connected with the conical pipe, and the large-diameter pipe, the conical pipe and the spherical shell are integrally formed. The outer side wall of the rotating spherical shell is rotationally connected with the inner side wall of the spherical shell, the top and the bottom of the rotating spherical shell are both provided with openings, and the position, away from the opening of the conical pipe, of the rotating spherical shell is connected with the suction pipe. According to the L-shaped plastic suction tube, the swinging angle of the suction tube can be controlled, cell colonies can be dispersed by using the outer side wall of the suction tube, and an operator can conveniently press the suction head to suck and transfer cells.

The invention belongs to the technical field of medical treatment, and discloses a method and a system for separating self red blood cells. Comprising the following steps: washing red blood cells with normal saline for three times, removing supernatant as much as possible under the condition of retaining an albuginea layer, retaining packed red blood cells, and uniformly mixing; adding at least two tubes of uniformly mixed packed red cells into the capillary tube by using a sample adding gun; sealing the bottom of the pipe with sealing mud; putting the capillary tube with the sealing mud at one end downwards into a blood collection tube without an anticoagulant, and tightening a blood collection tube cap; centrifuging for 5 minutes by using a blood sample centrifugal machine, wherein the centrifugal force setting condition is 2000g or 3500rpm; after centrifugation, the sample is carefully and vertically taken out from the blood collection tube without shaking; cutting off a capillary tube at one end without sealing mud, namely a proximal end, and transferring cells 5 mm away from the upper part of the capillary tube into a 12 * 75 mm test tube; after washing with normal saline, centrifuging, introducing the intercepted 5mm capillary tube and washing liquid into a waste liquid cylinder, and retaining the volume of red blood cells. According to the method, the operation cost and the equipment investment are effectively reduced by reducing the equipment requirement and shortening the centrifugal time.

The present disclosure relates in general to the field of genetic engineering of immune cells, specifically to mucosal-associated invariant T (MAIT) cells genetically engineered to express an exogeneous T cell receptor (TCR) and uses thereof. More specifically, the invention in embodiments thereof relates to cell compositions adapted for adoptive transfer cell therapy (ACT) providing for improved therapeutic modalities.

The following configuration was used to provide an automatic culture device, an automatic culture system, and a culture method with which it is possible to accurately feed a liquid regardless of the type of cell culture vessel, cell suspension vessel, or cell collection vessel used and with which it is possible to supply and collect cells with reduced physical damage to the cells by feeding a cell suspension between any containers without passing through the drive part of a pump mechanism.　An automatic culture device according to the present invention includes: a cell suspension storage vessel capable of storing a cell suspension; a cell processing vessel for processing a cell suspension fed from the cell suspension storage vessel; a flow path for connecting at least the cell suspension storage vessel and the cell processing vessel; a first buffer space connected to the flow path and having a predetermined pressure resistance; and a pressure source for drawing the cell suspension from the cell suspension storage vessel into the first buffer space by creating negative pressure within the first buffer space.

The system can include: a cell frame structure; a robot; and a motion planner. The system can optionally include a set of cell trays and a warehouse management system. The cell frame structure can include and / or define a set of payload storage cells; a set of transfer cells; a set of cell tray storage cells. However, the system can additionally or alternatively include any other suitable set of components. The system functions to automatically manage the storage of payloads within a grid-based structural system.

Disclosed herein is a microfluidic device for priming, collection and recovery of immune cells comprising: a) two separate fluidic channels: i) a first channel, wherein the first channel is infused with immune cells, and ii) a second channel, wherein the second channel acts as a collection reservoir for transmigrated cells, and; b) a microporous membrane interface, present between the first channel and the second channel, wherein the microporous membrane is functionalized on the first channel surface with adhesion molecules. Also disclosed are methods of creating enhanced cells, and using them in treatment of individuals in need thereof.