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493 results about "Cell preparation" patented technology

Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.
Owner:JUNO THERAPEUTICS INC

Preparation method of selective electrode contact heterojunction battery and heterojunction battery

The invention relates to the technical field of heterojunction solar cells, and particularly discloses a selective electrode contact heterojunction cell preparation method and a heterojunction cell, and the method comprises the steps: carrying out the first texturing of a silicon wafer substrate, carrying out the mask layer preparation of the textured silicon wafer, and carrying out the first patterning processing of the silicon wafer. Performing slotting treatment on two sides of the silicon wafer based on picosecond laser, performing secondary texturing on the silicon wafer, placing the cleaned silicon wafer in a second texturing mixed solution so as to generate a large textured pyramid structure on the inner bottom surface of a groove in the two sides of the silicon wafer, and removing the first mask layer from the silicon wafer after secondary texturing to obtain a second textured pyramid structure; sequentially depositing a plurality of layers including an intrinsic amorphous silicon layer and the like on the two surfaces of the silicon wafer, printing a second mask layer in a counterpoint manner in other areas of the silicon wafer which is provided with the groove in the first time of patterning based on exposure and development, electroplating a copper grid line on the silicon wafer, and sequentially removing the second mask layer and a copper seed layer on the silicon wafer after electroplating the grid line; the selective electrode contact copper grid line heterojunction solar cell is obtained, the problem of grid separation of the copper grid lines is solved, and the cell efficiency is improved.
Owner:STATE POWER INVESTMENT GRP NEW ENERGY TECH CO LTD

Intelligent quality evaluation system and method for whole process of cell preparation

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.
Owner:ST AIXIN MEDICAL TECHNOLOGY (SHANXI) CO LTD

Tandem cell, method for preparing the same, and photovoltaic module

The tandem cell includes a bottom cell including a substrate. The substrate has a first side and a second side, the first side has a first textured surface including at least one first protrusion structure. The bottom cell further includes a tunneling dielectric layer and a doped conductive layer. A surface of the doped conductive layer has a micro textured surface including at least one micro protrusion structure, and the micro protrusion structure is smaller than the first protrusion structure. The tandem cell further includes a composite layer conformal to the micro textured surface. The tandem cell further includes a top cell formed on the composite layer. The top cell includes a first transport layer, a perovskite substrate, a second transport layer, a transparent conductive layer, and an anti-reflection layer stacked sequentially. The first transport layer is conformal to the micro textured surface.
Owner:ZHEJIANG JINKO SOLAR CO LTD +1

Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.
Owner:JUNO THERAPEUTICS INC

Photovoltaic cell preparation method, photovoltaic cell, laminated cell and photovoltaic cell assembly

The invention relates to a photovoltaic cell preparation method, a photovoltaic cell, a laminated cell and a photovoltaic cell module. Providing an initial battery piece; depositing and forming a protective layer on the surface; carrying out laser treatment twice; printing and sintering to form an electrode; and carrying out at least two times of laser-assisted sintering on the electrode. According to the photovoltaic cell preparation method, the protection layer at the first area is removed, the doping concentration at the first area is improved, and at least two times of laser-assisted sintering are combined, so that on one hand, the metal contact area is optimized, the usage amount of silver paste is reduced, the material cost is reduced, and meanwhile, paste contact sintering is enhanced; damage caused by laser grooving doping is removed; and on the other hand, the doping concentration is optimized, the contact resistance is remarkably reduced, the open-circuit voltage and the filling factor are favorably improved, the shading loss is reduced, and the current collection efficiency is ensured, so that the photoelectric conversion efficiency and the reliability of the cell are remarkably improved, the production process is simplified, and the production cost is reduced.
Owner:ZHEJIANG JINKO SOLAR CO LTD

Preparation method of solar cell and solar cell

The invention discloses a preparation method of a solar cell and the solar cell. The preparation method comprises the following steps: removing a second mask layer on a first surface and a side surface of a silicon substrate by adopting a chained acid etching process; removing the second tunneling passivation structures on the first surface and the side surface of the silicon substrate by adopting a chained alkali etching process; and removing the second tunneling passivation structures in the first region and the third region of the second surface of the silicon substrate by adopting a groove type etching process, and forming a suede light trapping structure on the third region of the second surface of the silicon substrate and the first surface, and removing the first mask layer in the first area and the second mask layer in the second area on the second surface of the silicon substrate. According to the preparation method of the solar cell and the solar cell, the cell preparation process can be optimized, the environment is protected, the texture surface quality is improved, and the cell efficiency is improved.
Owner:扬州阿特斯太阳能电池有限公司

Sterile culture method and sterile culture system

The present invention relates to the technical field of biopharmaceutical apparatuses, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator, and a cell culture device; the cell preparation device comprises a preparation chamber, wherein a first sealing valve is arranged on a side wall of the preparation chamber; the incubator has a culture cavity, wherein a second sealing valve is arranged on a side wall of the culture cavity, and the second sealing valve is configured to work with the first sealing valve such that the incubator is hermetically docked with or separated from the cell preparation device; and the cell culture device is used for accommodating the incubator, supplying power and a gas to the incubator, and controlling a cell growth environment in the incubator. Because cells grow in a sterile environment all the time and the pollution risk is relatively low, no frequent sterilization is required. Normal growth of cells is avoided from being affected by frequent sterilization, and the culture time is saved. Therefore, the cell preparation and culture efficiency is relatively high.
Owner:SHANGHAI TOFFLON MEDICAL EQUIP CO LTD

Cell preparation processing method, device, system and medium

The invention discloses a cell preparation treatment method, system and medium, and the method comprises the following steps: carrying out cell amplification treatment on a to-be-cultured cell sap in a cell culture container to obtain a cultured cell sap; washing and concentrating the cultured cell sap in the cell culture container to obtain concentrated cell sap; and sub-packaging the concentrated cell sap in the cell culture vessel into a plurality of preparation bags. According to the invention, cell culture, cell washing and concentrating treatment and preparation sub-packaging are realized on the cell culture container, so that scattered arrangement of equipment is avoided, and the occupied space is reduced; the steps are sequentially completed through the cell culture container, the whole process from cell culture to cell preparation can be ensured to be performed in a sealed sterile environment, cell sap does not need to be transferred, and the cell culture quality is improved; the steps are sequentially and integrally completed through the cell culture container, manual intervention is reduced, the whole process from cell culture to cell preparation is intelligently carried out, and the cell culture efficiency is improved.
Owner:SHENZHEN CELLBRI BIO INNOVATION TECH CO LTD

Preparation process of bottom-coated perovskite layer

The invention relates to the technical field of photovoltaic cell preparation, and discloses a preparation process of a lower coated perovskite layer, and the process comprises the steps: obtaining a state effective value of an FTO glass film surface before the preparation of a lower interface layer, the preparation of a perovskite layer and the preparation of an upper interface layer; if the state effective value of the FTO glass film surface meets the preset requirement, carrying out a subsequent process for preparing a lower coated perovskite layer; the subsequent process comprises the steps of C60 layer evaporation, SnO2 layer preparation, P2 lineation, back electrode layer preparation, P3 lineation and edge cleaning. If the state effective value of the FTO glass film surface does not meet the preset requirement, carrying out circulating treatment on a preorder process for preparing the lower coated perovskite layer; the preorder process comprises the steps of glass cleaning, hole transport layer preparation, P1 scribing and Mark point scribing; wherein the preparation of the lower interface layer, the preparation of the perovskite layer and the preparation of the upper interface layer are carried out by adopting lower coating film preparation equipment, so that the yield of the preparation of the lower coating film perovskite layer is effectively ensured.
Owner:JIANGYIN JIZE WANJIA TECH CO LTD

Formula adjusting method and device for plasma enhanced chemical vapor deposition process

The invention relates to the field of photovoltaic cell preparation, and particularly provides a formula adjusting method and device for a plasma enhanced chemical vapor deposition process. The method comprises the following steps: acquiring a state parameter vector of a plasma enhanced chemical vapor deposition process machine in an operation process, and predicting a predicted film layer thickness corresponding to the state parameter vector; if the predicted film layer thickness does not meet the preset error, selecting k adjustable factors with top contribution degrees in the state parameter vectors as target adjustment factors; according to the gradient descending direction of the film layer thickness, searching a historical state parameter with the minimum Euclidean distance with the state parameter of each target regulation factor in a good product historical database, and taking the historical state parameter as a current formula parameter; and according to the state parameter vector corresponding to the current formula parameter, regenerating a predicted film layer thickness until the predicted film layer thickness meets a preset error, and taking the parameter in the current state parameter vector as a target formula. According to the technical scheme provided by the invention, the reject ratio of photovoltaic cell production is reduced.
Owner:ROBOTECHN INTELLIGENT TECH CO LTD

Preparation method of high-temperature-resistant lithium battery cell

The invention relates to the technical field of lithium batteries, in particular to a preparation method of a high-temperature-resistant lithium battery cell. The invention discloses a preparation method of a high-temperature-resistant lithium battery cell, which is characterized in that through full-chain synergy of modification of positive and negative electrode materials, electrolyte optimization and structural design, a positive electrode adopts nickel cobalt lithium aluminate with a core-shell structure, and a metal organic framework coordination layer, polyaniline-polyacrylic acid multilayer coating and foam aluminum foil 3D printing coating are combined. The negative electrode takes silicon-carbon core-shell particles as a substrate, and breaks through the expansion bottleneck of a silicon-based negative electrode through metal organic framework confinement coating, polyaniline-polyacrylic acid cross-linked network modification and foam copper foil functionalization. The electrolyte adopts a film-forming additive and lithium difluorophosphate to form a stable SEI film, and internal resistance increase at high temperature is inhibited. The aramid fiber coating diaphragm and the aluminum alloy shell are matched, so that the thermal stability of the battery cell is remarkably improved, the battery cell can stably work in a wide temperature range environment, high energy density and long cycle life are comprehensively realized, and key support is provided for a long-endurance and high-safety battery.
Owner:GUANGZHOU AOCHUANG TECHNOLOGY CO LTD

Perovskite / crystalline silicon laminated solar cell structure taking tin dioxide thin film as composite layer and preparation method of perovskite / crystalline silicon laminated solar cell structure

The invention relates to a perovskite / crystalline silicon laminated solar cell structure taking a tin dioxide thin film as a composite layer and a preparation method of the perovskite / crystalline silicon laminated solar cell structure, and belongs to the technical field of photovoltaics. According to the perovskite / crystalline silicon laminated solar cell structure, the tin dioxide thin film is used as a composite layer, so that the cost of cell preparation is reduced; in the preparation method of the perovskite / crystalline silicon laminated solar cell, the composite layer and the electron transport layer can adopt the same preparation method, so that the equipment cost in the preparation process is reduced; the perovskite / crystalline silicon laminated solar cell obtained by the cell structure and the preparation method provided by the invention is relatively low in preparation cost and relatively high in photoelectric conversion efficiency.
Owner:UNIV OF CHINESE ACAD OF SCI

Passivated perovskite thin film coating device and perovskite battery preparation system

The utility model relates to the field of perovskite cell related equipment, and provides a passivated perovskite film coating device and a perovskite cell preparation system. The passivated perovskite film coating device comprises a base table, an air knife mechanism, a nitrogen cylinder and an additive atomizer, a sliding mechanism is arranged on the base table, the air knife mechanism is slidably installed on the base table through the sliding mechanism, the nitrogen cylinder is provided with a nitrogen pipe, the additive atomizer is provided with an additive air pipe, and the top of the air knife mechanism is connected with a mixed air inlet pipe. An air outlet of the nitrogen pipe and an air outlet of the additive gas pipe are respectively communicated with the mixed gas inlet pipe. According to the passivated perovskite film coating device, the additive atomizer is used for atomizing a solution in which an additive is dissolved so that the solution can become gas, nitrogen and the atomized gas of the additive are mixed and then discharged from the air knife lip, and in the process that the air knife mechanism conducts blowing treatment on a liquid film, the liquid film can be blown out through the air knife. And doping of the additive to the perovskite or passivation treatment of the passivator to the surface of the perovskite is completed.
Owner:BEI JING SHUO WEI GUANG DIAN KE JI YOU XIAN GONG SI

Method for preparing plant single cell

According to the method, a plant explant callus culture mode is adopted, a carbon source containing a fluorophore is added into a culture medium, the callus which is provided with the fluorophore, thin in cell wall, easy to dissociate by a single enzyme and uniform in cell shape and size is generated through induction, and the cell dissociated by the single enzyme is detected by a flow cytometry, so that the cell quality is improved. The single cell purity and activity of the prepared leaf are 98.5% and 99.95% respectively, the clotting rate is only 1.5%, the cell purity and activity are improved by 32.6% and 21.05% respectively compared with those of a traditional technical scheme, and the clotting rate is reduced by 31.0%; according to the method, rice seeds are used as explants, seed single cells with the cell purity and activity being up to 99.5% and 100% respectively are obtained, and the limitation that the seed single cells cannot be prepared by a traditional enzymolysis method is broken through; besides, through co-induction of the safflower leaves and the rice seeds, the purity and activity of the prepared cells are as high as 99.98% and 100.0 respectively, and the limitation of single cell preparation caused by heterogeneity among species is overcome.
Owner:BEIJING EPSILON BIOTECHNOLOGY CO LTD

Quality control method and platform for NK cell preparation

The invention discloses a quality control method and platform for NK cell preparation, and relates to the technical field of cell preparation.The method comprises the steps that first preset culture environment information, EZH2 inhibitor weight parts and IL-15 fusion protein weight parts are processed through an NKG2D expression quantity prediction model, NKG2D predicted expression quantity is generated, when the NKG2D predicted expression quantity is inconsistent with target NKG2D expression quantity, directional optimization is conducted, and the NKG2D predicted expression quantity is obtained; obtaining culture environment baseline information meeting the target NKG2D expression quantity; when the culture environment monitoring time sequence information is inconsistent with the culture environment baseline information, an NK cell preparation quality control early warning signal is generated. The technical problem that in the large-scale preparation process, due to the uncertainty of the culture environment, the immunocompetence of the NK cells possibly has large difference is solved, and the purposes of ensuring the stability and consistency of the environment in the preparation process through a real-time monitoring and automatic early warning mechanism and improving the immunocompetence of the NK cells are achieved. Therefore, the stability and the consistency of the immunocompetence of the NK cells are improved.
Owner:中国人民解放军总医院京南医疗区

Resin-based hard carbon composite material and application thereof in preparation of button type half cell

The invention discloses a resin-based hard carbon composite material and application thereof in button type half-cell preparation. The method comprises the following steps: melting phenol, and adding a formaldehyde solution to prepare a mixed solution A; dispersing the carbon nano tube powder and hexadecyl trimethyl ammonium bromide in pure water, and grinding to obtain carbon nano tube dispersed slurry B; and adding the carbon nanotube dispersion slurry B into the mixed solution A to obtain a mixed solution C, adjusting the pH value to prepare a phenolic resin precursor solution, carrying out cross-linking curing to prepare a phenolic resin cured precursor, carrying out mechanical grinding, and putting the phenolic resin cured precursor into a tubular furnace to finally prepare the resin-based hard carbon composite material. Conductive carbon is introduced to the condensation polymerization end of phenol and formaldehyde, a communicated conductive network is formed in the composite material by regulating and controlling the material composition, the electron transmission distance between particles is shortened, the intrinsic conductivity of original resin carbon is improved, and the composite material has the advantages of high capacity, high first efficiency, high magnification and low ash content; wide application prospects are realized in the field of lithium ion battery negative electrode materials.
Owner:SINOSTEEL MAANSHAN INST OF MINING RES CO LTD +1

Hybrid passivated back-contact cell without doped silicon crystal layer arranged on front face thereof, and preparation method therefor

The present application belongs to the technical field of back-contact cell preparation, and particularly relates to a hybrid passivated back-contact cell without a doped silicon crystal layer arranged on a front face thereof, and a preparation method therefor. The method comprises: S101, forming in sequence a first semiconductor layer and a mask layer on a back face of a silicon wafer, both sides of which are polished; S102, performing first etching opening in the back face, so as to form second semiconductor opening areas; S103, performing texturing and cleaning; S104, depositing a first intrinsic amorphous silicon layer on the front face, and depositing a second semiconductor layer on the back face; S105, performing second etching opening in the second semiconductor layer on the back face; and S106, depositing a second transparent conductive film layer on the back face by using a magnetron sputtering process, and directly depositing a first transparent conductive film layer on the front face. The present application can effectively avoid a power attenuation problem caused by a PID effect occurring in a cell, so that one instance of PECVD coating can be reduced, thereby effectively reducing the reflectivity and reflection loss of a front face, and the quality of film layers is high, thereby improving the efficiency of conversion.
Owner:GOLD STONE (FUJIAN) ENERGY CO LTD

TopCon battery and TopCon battery preparation method

The invention discloses a TopCon cell and a TopCon cell preparation method, and belongs to the field of solar cells. The structure of the passivation layer is optimized, the doped polycrystalline silicon layer is reserved in the metal contact area, the doped polycrystalline silicon layer and the substrate form a high-low junction to provide field passivation, and ohmic contact is formed between the doped polycrystalline silicon layer and the electrode; a doped polycrystalline silicon carbide layer is arranged in a non-metal contact area, and the doped polycrystalline silicon carbide layer and a substrate form a heterojunction to provide field passivation and serve as a carrier transverse transmission layer. The parasitic absorption of the passivation layer is reduced by removing the doped polycrystalline silicon layer in the non-metal contact region. The light absorption coefficient of the doped polycrystalline silicon carbide layer is low and the doped polycrystalline silicon carbide layer is thin, so that parasitic absorption can be reduced. Ohmic contact is formed between the doped polycrystalline silicon layer in the reserved metal contact area and the electrode, so that low recombination loss can be ensured when the doped polycrystalline silicon layer is in contact with the metal grid line. Field passivation is formed in the metal contact area and the non-metal area, and the passivation effect of the whole surface of the substrate is guaranteed.
Owner:FENGYANG CHINT SOLAR TECHNOLOGY CO LTD

Preparation method of cells and application of cells in cell effectiveness detection

The invention relates to the field of bioengineering, in particular to a preparation method of cells and application of the cells in cell effectiveness detection. The invention provides a cell culture method which comprises the following steps: carrying out first stimulation, first culture, second stimulation, blocking, second culture and cleaning on pretreated cells to obtain the cells. The invention provides a method for determining the effectiveness of an immune cell preparation activated in vitro, according to the characteristics of the immune cell preparation activated in vitro, a two-step stimulation activation mode is adopted, the activation ratio of CD69 + CD44 + is used as a systematic internal control index of the method, and meanwhile, the mechanism of the immune cell preparation activated in vitro is combined to determine the effectiveness of the immune cell preparation activated in vitro. The Tc cell subpopulation secreting granzyme B is used as a method detection index, so that the indirect evaluation of the effectiveness of the immune cell preparation activated in vitro in human body is realized, and the method can be used for release detection of the immune cell preparation activated in vitro in human body.
Owner:YUNNAN PRECISION INSPECTION CO LTD

Photovoltaic cell string, photovoltaic cell panel and photovoltaic cell module

A photovoltaic cell string, a photovoltaic cell panel and a photovoltaic cell module, which are applied to the field of photovoltaic cell preparation. The cell string comprises: a plurality of cells, welding strips, which are arranged on first surfaces and second surfaces of the cells, and protective films, which are arranged on outer sides of the welding strips, wherein there is an overlapping region between adjacent cells among the plurality of cells; and the protective films located on preset cell surfaces of the adjacent cells extend to the overlapping region, such that by using the protective films, the stress generated between the cells and the welding strips in the overlapping region is reduced; the preset cell surfaces are the surfaces of the sides of the adjacent cells where the cells are stacked on each other; and the adjacent cells form an electrical connection by means of the welding strips located on the preset cell surfaces. In the present application, the protective films located on the preset surfaces of the cells in the adjacent cells extend to the overlapping region, such that the stress generated between the cells and the welding strip can be reduced.
Owner:CHINT NEW ENERGY TECH CO LTD

Heterojunction cell preparation method

The present invention relates to the technical field of solar cell preparation, and specifically provides a heterojunction cell preparation method and a heterojunction cell. The heterojunction cell preparation method comprises: providing a semiconductor substrate layer, at least one side of the semiconductor substrate layer being provided with a damage layer; performing first texturing treatment on the semiconductor substrate layer to remove some damage from the damage layer, such that the surface of the side of the semiconductor substrate layer having the damage layer forms an inverted pyramid textured surface, the inverted pyramid textured surface comprising a plurality of first microstructural units; and, after the first texturing treatment, performing second texturing treatment on the side of the semiconductor substrate layer where the inverted pyramid textured surface is formed, so as to form a plurality of second pyramids on the surfaces of the first microstructural units of the inverted pyramid textured surface, and remove remaining damage from the damage layer. The heterojunction cell preparation method of the present invention enables prepared cells to have high fill factors at low costs.
Owner:ANHUI HUASUN ENERGY CO LTD

Sterile culture method and sterile culture system

The invention relates to the technical field of biopharmaceutical equipment, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator and a cell culture device, the cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber; the incubator is provided with a culture chamber, a second sealing valve is arranged on the side wall of the culture chamber, and the second sealing valve is used for being matched with the first sealing valve, so that the incubator and the cell preparation device are in sealed butt joint or sealed separation; the cell culture device is used for accommodating the culture box, supplying power and gas to the culture box and controlling the cell growth environment in the culture box. As the cells always grow in the sterile environment, the pollution risk is low, frequent sterilization is not needed, the normal growth of the cells is prevented from being influenced by frequent sterilization, the culture time is saved, and the cell preparation and culture efficiency is high.
Owner:SHANGHAI TOFFLON MEDICAL EQUIP CO LTD

Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.
Owner:JUNO THERAPEUTICS INC

IBC photovoltaic cell

The invention discloses an IBC photovoltaic cell, and relates to the technical field of cell preparation, and the IBC photovoltaic cell comprises a silicon substrate, the back surface of the silicon substrate is provided with a first doped region, a first isolation groove G1, a second doped region and a second isolation groove G2 along the forward direction of an X axis, a first doped region, a third isolation groove G3, a second doped region and a fourth isolation groove G4 are arranged on the back face of the silicon substrate in the reverse direction of the Z axis, the first isolation groove G1 and the second isolation groove G2 are both perpendicular to the main grid, the width of the first isolation groove G1 and the width of the second isolation groove G2 are both 100-200 micrometers, the third isolation groove G3 and the width of the fourth isolation groove G4 are both parallel to the main grid, and the width of the third isolation groove G3 and the width of the fourth isolation groove G4 are both 120-320 micrometers. According to the invention, the width of the isolation groove is widened through the laser film opening parameter, so that when pollutants are attached to the groove, the conduction risk can be reduced, the production yield of the battery can be improved, and the widths of the first doped region and the second doped region are narrowed due to the widened width of the isolation groove, thereby weakening the parasitic absorption of the back surface and improving the performance of the battery. And the double-sided rate and the generating capacity of the centralized assembly are improved.
Owner:YIBIN YINGFA DERUI TECHNOLOGY CO LTD

Polymer fibers having a backbone having a positively charged component including a zwitterionic moiety

The present application relates to fibers of one or more biocompatible polymers having a diameter of 1 nm to 10,000 nm, wherein the polymer has a main chain including a positively charged component from a zwitterionic portion. In addition, the present application discloses an implantable therapeutic delivery system and a method for forming the same, the implantable therapeutic delivery system comprising a shell defining a chamber, wherein the shell is porous and is formed by the fibers. The interior of the shell includes a cell preparation that releases a therapeutic agent from the chamber. The implantable therapeutic delivery system can be used to treat diabetes.
Owner:CORNELL UNIVERSITY

Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.
Owner:JUNO THERAPEUTICS INC

Application of apoptotic cell pretreated macrophages in preparation of osteoarthritis treatment preparation, cell preparation and preparation method

The invention provides application of apoptotic cell pretreated macrophages in preparation of an osteoarthritis treatment preparation, a cell preparation and a preparation method, and relates to the technical field of biomedicine.In the embodiment of the invention, based on a mouse development model and an osteoarthritis (OA) model, it is found that in the area away from the cartilage surface, the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment, and the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment in the area away from the cartilage surface; in the macrophages, the content of the interment-related LAMP1 protein is obviously higher than that of the macrophages close to the cartilage surface area, and the expression results of TGF-beta 1 and LRG1 are respectively opposite and consistent with those of the LAMP1. The invention also finds that after the chemotactic effect of apoptotic cells on macrophages is weakened, the cellular burial effect is limited, and the cartilage matrix development disorder is caused. Based on development process inspiration, the invention provides a treatment strategy: bone marrow-derived macrophages pretreated by apoptotic cells are injected into articular cavities, and a good cartilage regeneration treatment effect can be achieved in a mouse arthritis model. In addition, the bone marrow-derived macrophages pretreated by apoptotic cells can still express TGF-beta1 after being injected into the body for 3 days, so that the effect of continuously promoting cartilage regeneration is achieved.
Owner:PEKING UNIV

Cell slide making and dyeing all-in-one machine

The invention relates to the technical field of biological experiment equipment, in particular to a cell slide-making and dyeing all-in-one machine which comprises a machine table, a centrifugal slide-making mechanism and a dyeing mechanism, the centrifugal slide-making mechanism and the dyeing mechanism are arranged on the machine table, and the centrifugal slide-making mechanism comprises a centrifugal disc driven by a driving device to rotate and a centrifugal test tube rotatably arranged on the centrifugal disc; the centrifugal test tube rotates around a rotating axis X in the first rotating direction when the centrifugal disc rotates, and a separating and fixing device is arranged between the centrifugal test tube and the centrifugal disc and can apply force in the first rotating direction to the bottom of the centrifugal test tube when centrifugal force borne by the centrifugal test tube is finished. The separating and fixing device is arranged between the centrifugal test tube and the centrifugal disc and can apply force in the first rotating direction to the bottom of the centrifugal test tube when centrifugal force borne by the centrifugal test tube is finished, so that upper-layer liquid in the centrifugal test tube can be directly poured out from a tube opening of the centrifugal test tube, and waste liquid can be conveniently discharged; and other parts cannot be polluted.
Owner:HEBEI QUANSHENG CELL BIOTECHNOLOGY CO LTD

Battery assembly based on rectangular silicon wafer and processing method thereof

PendingCN119300514Aimprove performanceImprove light absorption efficiencyFinal product manufactureElectrical batteryPotassium hydroxide
The invention relates to the technical field of photovoltaic cell preparation, in particular to a cell assembly based on a rectangular silicon wafer and a processing method of the cell assembly. Comprising the following steps: placing a monocrystalline silicon wafer in a potassium hydroxide solution, texturing, washing and drying to obtain a textured silicon wafer; sequentially performing low-pressure diffusion, SE laser, polishing and oxidizing annealing on the textured silicon wafer to obtain a silicon wafer A; sequentially depositing an aluminum oxide layer and a silicon nitride layer on the back surface of the silicon wafer A to obtain a silicon wafer B; depositing a silicon nitride layer on the front surface of the silicon wafer B to obtain a rectangular silicon wafer; performing laser grooving, screen printing and sintering on the rectangular silicon wafer to obtain a single battery piece; the single battery pieces are connected in series through a welding strip to obtain a battery string; (3) sequentially laying glass, POE / EPE, a battery string, EVA and grid glazed backboard glass, sealing the edge by an edge sealing tape machine, and then, carrying out lamination, framing and junction box installation in the next process; and thus, a rectangular silicon wafer battery assembly is obtained.
Owner:ECONESS ENERGY