284 results about "Cell preparation" patented technology

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

The invention relates to the field of photovoltaic cell preparation, and particularly provides a formula adjusting method and device for a plasma enhanced chemical vapor deposition process. The method comprises the following steps: acquiring a state parameter vector of a plasma enhanced chemical vapor deposition process machine in an operation process, and predicting a predicted film layer thickness corresponding to the state parameter vector; if the predicted film layer thickness does not meet the preset error, selecting k adjustable factors with top contribution degrees in the state parameter vectors as target adjustment factors; according to the gradient descending direction of the film layer thickness, searching a historical state parameter with the minimum Euclidean distance with the state parameter of each target regulation factor in a good product historical database, and taking the historical state parameter as a current formula parameter; and according to the state parameter vector corresponding to the current formula parameter, regenerating a predicted film layer thickness until the predicted film layer thickness meets a preset error, and taking the parameter in the current state parameter vector as a target formula. According to the technical scheme provided by the invention, the reject ratio of photovoltaic cell production is reduced.

The invention relates to the technical field of lithium batteries, in particular to a preparation method of a high-temperature-resistant lithium battery cell. The invention discloses a preparation method of a high-temperature-resistant lithium battery cell, which is characterized in that through full-chain synergy of modification of positive and negative electrode materials, electrolyte optimization and structural design, a positive electrode adopts nickel cobalt lithium aluminate with a core-shell structure, and a metal organic framework coordination layer, polyaniline-polyacrylic acid multilayer coating and foam aluminum foil 3D printing coating are combined. The negative electrode takes silicon-carbon core-shell particles as a substrate, and breaks through the expansion bottleneck of a silicon-based negative electrode through metal organic framework confinement coating, polyaniline-polyacrylic acid cross-linked network modification and foam copper foil functionalization. The electrolyte adopts a film-forming additive and lithium difluorophosphate to form a stable SEI film, and internal resistance increase at high temperature is inhibited. The aramid fiber coating diaphragm and the aluminum alloy shell are matched, so that the thermal stability of the battery cell is remarkably improved, the battery cell can stably work in a wide temperature range environment, high energy density and long cycle life are comprehensively realized, and key support is provided for a long-endurance and high-safety battery.

The invention discloses a TopCon cell and a TopCon cell preparation method, and belongs to the field of solar cells. The structure of the passivation layer is optimized, the doped polycrystalline silicon layer is reserved in the metal contact area, the doped polycrystalline silicon layer and the substrate form a high-low junction to provide field passivation, and ohmic contact is formed between the doped polycrystalline silicon layer and the electrode; a doped polycrystalline silicon carbide layer is arranged in a non-metal contact area, and the doped polycrystalline silicon carbide layer and a substrate form a heterojunction to provide field passivation and serve as a carrier transverse transmission layer. The parasitic absorption of the passivation layer is reduced by removing the doped polycrystalline silicon layer in the non-metal contact region. The light absorption coefficient of the doped polycrystalline silicon carbide layer is low and the doped polycrystalline silicon carbide layer is thin, so that parasitic absorption can be reduced. Ohmic contact is formed between the doped polycrystalline silicon layer in the reserved metal contact area and the electrode, so that low recombination loss can be ensured when the doped polycrystalline silicon layer is in contact with the metal grid line. Field passivation is formed in the metal contact area and the non-metal area, and the passivation effect of the whole surface of the substrate is guaranteed.

The present invention relates to the technical field of solar cell preparation, and specifically provides a heterojunction cell preparation method and a heterojunction cell. The heterojunction cell preparation method comprises: providing a semiconductor substrate layer, at least one side of the semiconductor substrate layer being provided with a damage layer; performing first texturing treatment on the semiconductor substrate layer to remove some damage from the damage layer, such that the surface of the side of the semiconductor substrate layer having the damage layer forms an inverted pyramid textured surface, the inverted pyramid textured surface comprising a plurality of first microstructural units; and, after the first texturing treatment, performing second texturing treatment on the side of the semiconductor substrate layer where the inverted pyramid textured surface is formed, so as to form a plurality of second pyramids on the surfaces of the first microstructural units of the inverted pyramid textured surface, and remove remaining damage from the damage layer. The heterojunction cell preparation method of the present invention enables prepared cells to have high fill factors at low costs.

The invention discloses an IBC photovoltaic cell, and relates to the technical field of cell preparation, and the IBC photovoltaic cell comprises a silicon substrate, the back surface of the silicon substrate is provided with a first doped region, a first isolation groove G1, a second doped region and a second isolation groove G2 along the forward direction of an X axis, a first doped region, a third isolation groove G3, a second doped region and a fourth isolation groove G4 are arranged on the back face of the silicon substrate in the reverse direction of the Z axis, the first isolation groove G1 and the second isolation groove G2 are both perpendicular to the main grid, the width of the first isolation groove G1 and the width of the second isolation groove G2 are both 100-200 micrometers, the third isolation groove G3 and the width of the fourth isolation groove G4 are both parallel to the main grid, and the width of the third isolation groove G3 and the width of the fourth isolation groove G4 are both 120-320 micrometers. According to the invention, the width of the isolation groove is widened through the laser film opening parameter, so that when pollutants are attached to the groove, the conduction risk can be reduced, the production yield of the battery can be improved, and the widths of the first doped region and the second doped region are narrowed due to the widened width of the isolation groove, thereby weakening the parasitic absorption of the back surface and improving the performance of the battery. And the double-sided rate and the generating capacity of the centralized assembly are improved.

There are provided measures for enabling / realizing efficient / favorable data forwarding in lower-layer mobility in / for a mobile communication system, namely a radio access network of a mobile communication system, such as L1 / L2 centric mobility. Such measures exemplarily comprise that a lower-layer entity in a radio access network of a mobile communication system obtains a configuration of lower-layer mobility and data forwarding in lower-layer mobility from a higher-layer control plane entity, initiates data forwarding to at least one target cell of lower-layer mobility in accordance with the configuration, and notifies a higher-layer user plane entity of initiation of data forwarding, wherein the at least one target cell of lower-layer mobility is identified. The configuration may indicate on-time data forwarding, wherein data forwarding to a target cell is triggered when a serving cell change decision is completed, or ay indicate early data forwarding upon preparation of candidate target cells for lower-layer mobility, wherein data forwarding to at least one of one or more candidate target cells is triggered when candidate target cells preparation is completed.

1. Name of the design product: packing box (cell preparation). 2. Use of the design product: the design product is used for packing and storing cell liquid. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The present application relates to the technical field of biological cell culture, and particularly relates to a three-dimensional cell culture device with automatic liquid replacement function, comprising: a box body; a rotating assembly, a liquid replacement assembly and a control display assembly are integrally installed in the box body. The three-dimensional cell culture device with automatic liquid replacement function provided by the present application integrates microgravity simulation and liquid replacement function in a relatively closed culture box body space, ensures that the culture environment in the cell culture process has high stability by not excessively affecting the air flow, temperature and humidity environment, etc. in the culture box body during liquid replacement; the device for replacing culture solution does not need to rotate, effectively releases the culture space while reducing the energy consumption required in the rotation process, can load multiple culture bottles for three-dimensional culture and automatic liquid replacement at the same time, and has high potential for large-scale cell preparation.

The utility model relates to the field of medical instruments, and discloses a centrifugal mechanism of a full-automatic liquid-based cell slide-making and dyeing all-in-one machine, which comprises a liquid-based cell slide-making and dyeing all-in-one machine body, and a top cover is hinged on the outer wall of the liquid-based cell slide-making and dyeing all-in-one machine body. The inner wall of the liquid-based cell slide preparation and dyeing all-in-one machine body is slidably connected with a sedimentation cabin, a test tube is placed at the top of the sedimentation cabin, the outer wall of a top cover is rotatably connected with a gear, the outer wall of the gear is fixedly connected with a cleaning rod, and the inner wall of the top cover is fixedly connected with a sealing ring plate; a rotating disc is slidably connected into the inner wall of the sealing ring plate. According to the cleaning device, the ultrasonic transducer at the bottom end of the cleaning rod is matched with physical cleaning of the cleaning rod, stubborn stains and cell residues on the inner wall and the bottom of a test tube can be efficiently removed, the cleaning effect is remarkably improved, it is guaranteed that a sample is not interfered by impurities in subsequent liquid-based cell preparation dyeing operation, and the detection accuracy is improved.

The application provides a kind of HLA-A24:02 APC cell preparation method and application, it is related to cell preparation technical field.The present application constructs the APC cell (K562-HLA-A24:02) that can replace HLA-A24:02 subtype DC cell, fills the blank of HLA-A24:02 subtype special engineering APC cell, and the cell can realize the antigen presentation function consistent with natural HLA-A24:02 subtype DC cell, provides special tool cell for the immune research for the HLA subtype;The present application constructs APC cell with K562 cell line as base, K562 cell can be in vitro permanent passage amplification, without repeatedly separating and inducing DC cell from human peripheral blood, reduces the use amount of peripheral blood, reduces the raw material dependence and cost of cell acquisition, while avoiding the influence of individual difference of peripheral blood source on experimental results.

The utility model discloses a cell transfer bag with a novel structure, which comprises a transfer bag which is respectively connected with an input port, a liquid inlet conduit and an output port, and is characterized by further comprising a sampling bag which is communicated with the transfer bag through a plastic hose and is a closed bag body. The cell preparation sampling device is novel in structure and high in practicability, the upper end of the transfer bag is connected with the sampling bag through the plastic hose, when a cell preparation needs to be sampled, 1-2 ml of cell suspension is manually squeezed into the sampling bag, then the plastic hose between the sampling bag and the transfer bag is subjected to heat sealing, after heat sealing is completed, the sampling bag is taken down, and then cell activity and cell quantity detection can be conducted; the sampling is convenient and sterile.

The invention relates to the technical field of biomedical treatment, particularly provides a cell preparation and subpackaging method, and aims to solve the problem that an existing cell preparation and subpackaging method is easy to cause cell burn. Specifically, the cell preparation and subpackaging method comprises the following steps: subpackaging cell sap into a plurality of cryopreservation bags; and injecting a cryopreservation solution into the cryopreservation bag, and in the process of injecting the cryopreservation solution, enabling the cryopreservation bag to reciprocate, so that the cell sap in the cryopreservation bag is uniformly mixed with the cryopreservation solution. According to the cryopreservation device, the cell sap can be subpackaged into the multiple cryopreservation bags, then the cryopreservation liquid is injected into each cryopreservation bag, and due to the fact that the number of the cell sap in each cryopreservation bag is small, heat generated when the cryopreservation liquid is injected is small, cell burn can be avoided, and the cell viability is improved.

The invention provides an NKT cell preparation as well as a preparation method and application thereof, and belongs to the field of biomedical treatment. The NKT cell preparation provided by the invention comprises NKT cells, a compound electrolyte injection, 5-10% of human serum albumin, 5-10% of raffinose and 2-5% of lysine, wherein the concentration of the NKT cells in the cell preparation is 1.0 * 10 < 6 >-1.0 * 10 < 8 > / mL; the prepared finished product preparation is long in shelf life, can be stably stored at 4 DEG C for more than 144 hours, and can be stably stored at-80 DEG C for at least 12 months.

The utility model relates to the technical field of cell slide, and discloses a gun head locking mechanism of a cell slide dyeing machine, which comprises a dyeing machine box, the top of the dyeing machine box is in sliding connection with a liquid storage pipe, the bottom of the liquid storage pipe is fixedly connected with a gun head shell, a vacuum generating device is arranged in the gun head shell, and the gun head shell is fixedly connected with the liquid storage pipe. The rear side of the vacuum generating device communicates with an adsorption cavity, a sealing column is fixedly connected to the bottom of the vacuum generating device, a plurality of air flow channels are formed in the top of the sealing column, a connecting pipe is fixedly connected to the middle of the sealing column, and the top of the connecting pipe communicates with the bottom of the liquid storage pipe. According to the pipette fixing device, the pipette is preliminarily clamped from the bottom of the connecting pipe through the lantern ring, suction force is generated through the vacuum generating device, the fixing effect is achieved, excessive mechanical extrusion and damage to the pipette cannot be caused by the fixing mode, the service life can be prolonged, and meanwhile it is avoided that the experiment result is affected by liquid pollution.

The utility model discloses a centrifugal liquid-based cell sheet making machine which comprises a machine body, the machine body is provided with a centrifugal cavity, a rotating disc and a driving mechanism for driving the rotating disc to rotate are installed in the centrifugal cavity, a plurality of containing grooves distributed in the circumferential direction are formed in the rotating disc, and a sheet making clamp is placed in any containing groove; a synchronous fixing mechanism and a synchronous adjusting mechanism are further arranged on the rotating disc, the synchronous fixing mechanism is used for fixing the slide making clamps in the containing grooves, and the synchronous adjusting mechanism is used for fixing the slides in the slide containing bins; the slide making clamp is provided with the synchronous fixing mechanism, all slide making clamps can be fixed under the condition that the slide making clamp is detachable, so that the installation time is saved, in addition, the slide making clamp is further provided with the synchronous adjusting mechanism, a plurality of slides can be fixed synchronously, and adaptive fixing can be carried out according to the thicknesses of the used slides.

The application discloses a kind of mesenchymal stem cell ball fractional dissociation digestive juice, its preparation method and application, it is related to cell preparation and three-dimensional cell culture technical field, specifically at to provide C liquid, D liquid and E liquid three kinds of gradient concentration working solution.When dissociating, the mesenchymal stem cell ball to be dissociated is determined its average particle size;According to the average particle size, select C liquid, D liquid or E liquid as digestive juice as described above;The selected digestive juice is mixed with the mesenchymal stem cell ball, and digestion reaction is carried out under oscillation condition, and single cell suspension is obtained.The application establishes the hierarchical matching rule of mesenchymal stem cell ball particle size and specific digestive juice concentration, and optimizes oscillation digestion condition, so as to maintain high activity rate and complete phenotype characteristics while efficiently dissociating, realizes the standardization of process and the controllable of dissociation quality, provides stable and reliable solution for the large-scale preparation and quality control of mesenchymal stem cell ball.

An apparatus for preparing a cell is provided. The apparatus includes an electrode reel from which an electrode sheet is unwound, wherein the electrode sheet is configured to form a plurality of electrodes, a separator reel from which a separator sheet is unwound, wherein the separator sheet is configured to stack with the electrode while covering the electrode by being folded when the electrode is stably placed, a table having an upper surface on which the electrode and the separator sheet are stably placed, and a nozzle configured to apply an adhesive to at least a portion of a region of the separator sheet stably placed on the table.

The present application relates to cell preparation process in the field of bioengineering, disclose a kind of high-efficiency preparation mouse primary hepatocyte method, comprising: perfusion needle is placed in reverse direction to liver direction through inferior vena cava, utilize the blood vessel clamp with elastic micro-tooth damping surface to lock the perfusion needle and inferior vena cava wall;The overflow damping of hepatic portal vein outflow end is dynamically regulated by blood vessel clamp, to construct controlled positive pressure permeation environment in liver vascular bed;Collagenase solution is radially permeated to liver parenchyma using the environment, the present application makes enzyme solution radial deflection and cross blood sinus barrier into di's cavity by constructing micro-pressure environment, eliminate the digestion dead zone originally due to flow path preference, ensure that enzyme molecule and extracellular matrix are uniformly contacted in global dimension, realize the collaborative promotion of tissue cutting efficiency and total amount of cell release, ensure that the output cell population has highly consistent physiological state.

The embodiment of the utility model provides a printing roller and a grid line printing device, and relates to the technical field of photovoltaic cell preparation. Grooves used for forming printing patterns are formed in the surface of the printing roller, the surface of the printing roller is used for making contact with the surface of a silicon wafer, and slurry in the grooves is used for being printed on the surface of the silicon wafer. During use, the printing roller can be manually driven to rotate on the surface of a silicon wafer, and an automatic driving device can also be adopted to drive the printing roller to rotate on the surface of the silicon wafer, so that the slurry in the groove is printed on the surface of the silicon wafer, and the printing work is completed. Compared with a silk-screen printing mode in the prior art, the printing roller has the advantages that the grooves used for forming the printing patterns are directly formed in the surface of the printing roller, the printing structure is simplified, the printing roller can continuously conduct printing work, the service life is long, and the consumption cost is reduced.

The application provides an immune cell preparation method capable of reversing tumor microenvironment immunosuppression signals and application thereof. The method for reversing the inhibitory signals is to replace the intracellular segment of a TIGIT receptor with a 4-1BB costimulatory domain and an IL-18R and a CD3 intracellular segment, and the immune cells expressing the chimeric antigen receptor recognize CD155 in a tumor microenvironment through TIGIT, avoid loss of function or exhaustion of the immune cells, and stimulate the immune cells to exert stronger tumor killing activity.

The invention relates to the technical field of biological engineering, and discloses a method for enhancing the urea circulation function of a 3D biological printing liver organ, which comprises the following steps: a cell preparation step: through a genetic engineering technology, enabling mitochondria of liver cells to express a light-driven proton pump, and enabling intracellular expression of a fluorescent probe for reporting ammonia concentration; an organ construction step: mixing the cells with hydrogel to prepare bio-ink, and constructing a three-dimensional liver organ by using a 3D biological printing technology; a system integration step: placing the liver organ in an integrated photoelectric regulation and control system capable of monitoring a fluorescence signal in real time and applying illumination; and a closed-loop enhancement step: the system monitors the ammonia concentration in real time, and when the ammonia concentration exceeds a preset threshold value, illumination is automatically applied to activate the proton pump, so that the urea circulation function is enhanced as required. Through closed-loop light control and a subcellular organelle energy channel, on-demand, efficient and high-temporal-spatial-resolution dynamic enhancement of the liver organ urea circulation function is achieved.

One approach to the exploration method includes calculating a TAD score representing transcriptional activity for each TAD present in the genome and selecting TADs based on the TAD scores. Another approach includes analyzing a genome obtained from cells expressing a foreign gene and selecting the boundaries of regions containing the foreign gene. Cell preparation involves inserting the target gene into the regions identified by the exploration method. Cell sorting involves calculating a TAD score representing transcriptional activity for each TAD present in the genome, selecting TADs based on the TAD scores, and selecting cells containing the target gene in the selected TADs. The cells are derived from Chinese hamsters and are cells in which the target gene has been inserted into at least one specified region. The method for manufacturing the cell product includes culturing the cells to express the target gene.

Provided herein are methods related to co-packaging of multiple rAAV particles. e.g., by introducing multiple nucleic acid vectors encoding proteins or polypeptides or RNAs of interest into a single cell preparation.

The invention relates to the technical field of cell preparation, and particularly discloses a cell drug and a preparation method thereof.The cell drug comprises mesenchymal cells and pharmaceutically acceptable auxiliary materials; the mesenchymal cell is characterized in that the expression of SOX2, DPPA4 and MYCN genes is negative; the auxiliary material comprises a sodium chloride solution containing albumin; the concentration of the sodium chloride solution is 0.1%-2%; the content of the albumin in the sodium chloride solution is 0.1%-10%. The cell medicine is simple in composition and good in auxiliary material compatibility, the motility of active ingredients can be protected, and the safety and reliability of the cell medicine are remarkably improved by optimizing the preparation process.

The invention discloses a cell composition for promoting hair regeneration and application thereof, and belongs to the field of biological medicine, the active component of the cell composition comprises a mixture of hair papilla cells, keratinocytes and stem cell growth factors; wherein the number ratio of the hair papilla cells to the keratinocytes is (1-3): (1-3), and the stem cell growth factor mixture comprises bFGF, KGF, HGF, VEGF, TGF-beta1 and PDGF. The cell preparation disclosed by the invention can be used for improving the hair growth effectiveness and solving different types of alopecia symptoms except for androgen alopecia, has a remarkable treatment effect on androgen alopecia, and has a remarkable effect on treating various alopecia symptoms. The traditional Chinese medicine composition has treatment effects on alopecia areata, alopecia in a resting period, alopecia in a growing period, scar alopecia and androgen-derived alopecia.

The utility model relates to the technical field of cell slide dyeing, and discloses a gun head transverse spacing adjusting mechanism of a cell slide dyeing machine, which comprises a guide rail, the inner wall of the guide rail is in threaded connection with a screw rod, the right side of the guide rail is fixedly connected with a positioning frame, and the right end of the screw rod is fixedly connected with a fixed disc I; a second fixing disc is fixedly connected to the right side of the first fixing disc, a plurality of transmission blocks are fixedly connected to the outer wall of the second fixing disc, an inner gear ring is slidably connected to the inner wall of the positioning frame, a sun gear is arranged on the inner wall of the inner gear ring, and a plurality of planetary gears are connected to the outer wall of the sun gear in a meshed mode. And the left side of the planetary gear is fixedly connected with a planetary carrier. According to the utility model, the telescopic rod is started to push the asynchronous motor to slide in the positioning frame, and the asynchronous motor is clamped with the transmission groove in the transmission disc on the outer wall of the transmission shaft II, so that the purpose of adjusting the fineness while transversely moving is achieved, the tablet production precision is improved, and the practicability is improved.

The invention discloses a low-warping-degree thin film solar cell manufacturing method, and aims to solve the warping problem caused by mismatching of material intrinsic stress and thermal expansion coefficient in cell preparation. According to the technical scheme, when an epitaxial wafer is preprocessed, an Au contact layer, a compressive / tensile stress metal alternating film layer and an Au bonding layer are sequentially evaporated; when the thin film supporting substrate is pretreated, a Cr-Ti-Pt-Au bonding layer is evaporated on one side, a silicon nitride, silicon oxide or aluminum oxide warping adjustment layer is prepared on the other side, and photoetching trepanning and evaporation of a back contact electrode are required to be performed on the warping adjustment layer for an electrode structure on the different side; and after contraposition permanent bonding, a finished product is obtained through temporary bonding, substrate removal, photoetching coating, antireflection film evaporation and alloy treatment. Internal and external stress is offset through the double-layer warping adjusting structure, the same-side electrode structure and the different-side electrode structure are adapted, the process is mature and easy to popularize, the warping degree of the battery is effectively reduced, and the product yield and the use reliability are remarkably improved.

The invention provides a synergistic engineering tumor infiltration lymphocyte and a preparation method thereof, and belongs to the technical field of biological medicines. The engineering tumor infiltrating lymphocyte capable of simultaneously expressing the activated fusion protein and the retention fusion protein is constructed, so that the recognition and killing capabilities of the engineering tumor infiltrating lymphocyte on tumor cells are remarkably improved, and meanwhile, the self-killing of the cells in an activated state is effectively avoided, so that the continuity and the safety of treatment are enhanced. The cell preparation shows a good application prospect in solid tumor treatment, and a new effective strategy is provided for overcoming the limitation of the existing immune cell therapy.

The invention discloses an IBC photovoltaic cell and a preparation method thereof, and relates to the technical field of cell preparation, the IBC photovoltaic cell comprises a silicon substrate, the back surface of the silicon substrate is provided with a plurality of back surface first doped regions, back surface second doped regions and isolation groove regions which are alternately arranged, the isolation groove regions are groove isolation G regions, and the groove isolation G regions are arranged on the back surface of the silicon substrate. The back metallization circuit pattern of the IBC photovoltaic cell adopts an 0BB connection circuit pattern without a main grid connection line. According to the invention, an 0BB electrocoppering line without a main grid connecting line is adopted, so that a large number of trench isolation G regions do not need to be arranged at the positions of the patterned main grids I and II, the processing flow is simplified, and a cylindrical contact channel is formed through processes such as laser film opening and acid cleaning. The aluminum-titanium alloy barrier layer, the copper seed layer, the copper conductive layer and the antioxidant tin layer are adopted to construct the metal electrode, so that the non-silicon cost of the IBC photovoltaic cell is further reduced, meanwhile, the contact resistance between aluminum and polycrystalline silicon is lower, and compared with a silver-coated copper wire screen printing process, the electrical property of the cell is optimized.