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172 results about "Cell preparation" patented technology

Formula adjusting method and device for plasma enhanced chemical vapor deposition process

The invention relates to the field of photovoltaic cell preparation, and particularly provides a formula adjusting method and device for a plasma enhanced chemical vapor deposition process. The method comprises the following steps: acquiring a state parameter vector of a plasma enhanced chemical vapor deposition process machine in an operation process, and predicting a predicted film layer thickness corresponding to the state parameter vector; if the predicted film layer thickness does not meet the preset error, selecting k adjustable factors with top contribution degrees in the state parameter vectors as target adjustment factors; according to the gradient descending direction of the film layer thickness, searching a historical state parameter with the minimum Euclidean distance with the state parameter of each target regulation factor in a good product historical database, and taking the historical state parameter as a current formula parameter; and according to the state parameter vector corresponding to the current formula parameter, regenerating a predicted film layer thickness until the predicted film layer thickness meets a preset error, and taking the parameter in the current state parameter vector as a target formula. According to the technical scheme provided by the invention, the reject ratio of photovoltaic cell production is reduced.
Owner:ROBOTECHN INTELLIGENT TECH CO LTD

Heterojunction cell preparation method

The present invention relates to the technical field of solar cell preparation, and specifically provides a heterojunction cell preparation method and a heterojunction cell. The heterojunction cell preparation method comprises: providing a semiconductor substrate layer, at least one side of the semiconductor substrate layer being provided with a damage layer; performing first texturing treatment on the semiconductor substrate layer to remove some damage from the damage layer, such that the surface of the side of the semiconductor substrate layer having the damage layer forms an inverted pyramid textured surface, the inverted pyramid textured surface comprising a plurality of first microstructural units; and, after the first texturing treatment, performing second texturing treatment on the side of the semiconductor substrate layer where the inverted pyramid textured surface is formed, so as to form a plurality of second pyramids on the surfaces of the first microstructural units of the inverted pyramid textured surface, and remove remaining damage from the damage layer. The heterojunction cell preparation method of the present invention enables prepared cells to have high fill factors at low costs.
Owner:ANHUI HUASUN ENERGY CO LTD

IBC photovoltaic cell

The invention discloses an IBC photovoltaic cell, and relates to the technical field of cell preparation, and the IBC photovoltaic cell comprises a silicon substrate, the back surface of the silicon substrate is provided with a first doped region, a first isolation groove G1, a second doped region and a second isolation groove G2 along the forward direction of an X axis, a first doped region, a third isolation groove G3, a second doped region and a fourth isolation groove G4 are arranged on the back face of the silicon substrate in the reverse direction of the Z axis, the first isolation groove G1 and the second isolation groove G2 are both perpendicular to the main grid, the width of the first isolation groove G1 and the width of the second isolation groove G2 are both 100-200 micrometers, the third isolation groove G3 and the width of the fourth isolation groove G4 are both parallel to the main grid, and the width of the third isolation groove G3 and the width of the fourth isolation groove G4 are both 120-320 micrometers. According to the invention, the width of the isolation groove is widened through the laser film opening parameter, so that when pollutants are attached to the groove, the conduction risk can be reduced, the production yield of the battery can be improved, and the widths of the first doped region and the second doped region are narrowed due to the widened width of the isolation groove, thereby weakening the parasitic absorption of the back surface and improving the performance of the battery. And the double-sided rate and the generating capacity of the centralized assembly are improved.
Owner:YIBIN YINGFA DERUI TECHNOLOGY CO LTD

Three-dimensional cell culture device with automatic liquid exchange function

The present application relates to the technical field of biological cell culture, and particularly relates to a three-dimensional cell culture device with automatic liquid replacement function, comprising: a box body; a rotating assembly, a liquid replacement assembly and a control display assembly are integrally installed in the box body. The three-dimensional cell culture device with automatic liquid replacement function provided by the present application integrates microgravity simulation and liquid replacement function in a relatively closed culture box body space, ensures that the culture environment in the cell culture process has high stability by not excessively affecting the air flow, temperature and humidity environment, etc. in the culture box body during liquid replacement; the device for replacing culture solution does not need to rotate, effectively releases the culture space while reducing the energy consumption required in the rotation process, can load multiple culture bottles for three-dimensional culture and automatic liquid replacement at the same time, and has high potential for large-scale cell preparation.
Owner:PEKING UNION MEDICAL COLLEGE HOSPITAL

Centrifugal mechanism of full-automatic liquid-based cell flaking and dyeing all-in-one machine

The utility model relates to the field of medical instruments, and discloses a centrifugal mechanism of a full-automatic liquid-based cell slide-making and dyeing all-in-one machine, which comprises a liquid-based cell slide-making and dyeing all-in-one machine body, and a top cover is hinged on the outer wall of the liquid-based cell slide-making and dyeing all-in-one machine body. The inner wall of the liquid-based cell slide preparation and dyeing all-in-one machine body is slidably connected with a sedimentation cabin, a test tube is placed at the top of the sedimentation cabin, the outer wall of a top cover is rotatably connected with a gear, the outer wall of the gear is fixedly connected with a cleaning rod, and the inner wall of the top cover is fixedly connected with a sealing ring plate; a rotating disc is slidably connected into the inner wall of the sealing ring plate. According to the cleaning device, the ultrasonic transducer at the bottom end of the cleaning rod is matched with physical cleaning of the cleaning rod, stubborn stains and cell residues on the inner wall and the bottom of a test tube can be efficiently removed, the cleaning effect is remarkably improved, it is guaranteed that a sample is not interfered by impurities in subsequent liquid-based cell preparation dyeing operation, and the detection accuracy is improved.
Owner:SHENZHEN RONGYI MEDICAL EQUIPMENT CO LTD

Method for preparing HLA-A24:02 APC cells and application thereof

PendingCN122146790AFermentationHybrid peptidesInducer CellsK562 cells
The application provides a kind of HLA-A24:02 APC cell preparation method and application, it is related to cell preparation technical field.The present application constructs the APC cell (K562-HLA-A24:02) that can replace HLA-A24:02 subtype DC cell, fills the blank of HLA-A24:02 subtype special engineering APC cell, and the cell can realize the antigen presentation function consistent with natural HLA-A24:02 subtype DC cell, provides special tool cell for the immune research for the HLA subtype;The present application constructs APC cell with K562 cell line as base, K562 cell can be in vitro permanent passage amplification, without repeatedly separating and inducing DC cell from human peripheral blood, reduces the use amount of peripheral blood, reduces the raw material dependence and cost of cell acquisition, while avoiding the influence of individual difference of peripheral blood source on experimental results.
Owner:赣州市人民医院 +1

Gun head locking mechanism of cell slide-making dyeing machine

The utility model relates to the technical field of cell slide, and discloses a gun head locking mechanism of a cell slide dyeing machine, which comprises a dyeing machine box, the top of the dyeing machine box is in sliding connection with a liquid storage pipe, the bottom of the liquid storage pipe is fixedly connected with a gun head shell, a vacuum generating device is arranged in the gun head shell, and the gun head shell is fixedly connected with the liquid storage pipe. The rear side of the vacuum generating device communicates with an adsorption cavity, a sealing column is fixedly connected to the bottom of the vacuum generating device, a plurality of air flow channels are formed in the top of the sealing column, a connecting pipe is fixedly connected to the middle of the sealing column, and the top of the connecting pipe communicates with the bottom of the liquid storage pipe. According to the pipette fixing device, the pipette is preliminarily clamped from the bottom of the connecting pipe through the lantern ring, suction force is generated through the vacuum generating device, the fixing effect is achieved, excessive mechanical extrusion and damage to the pipette cannot be caused by the fixing mode, the service life can be prolonged, and meanwhile it is avoided that the experiment result is affected by liquid pollution.
Owner:XIAOGANG HONGXIANG BIOLOGICAL TECH CO LTD

Fractionated dissociation digestive solution of mesenchymal stem cell spheres, preparation method and application thereof

The application discloses a kind of mesenchymal stem cell ball fractional dissociation digestive juice, its preparation method and application, it is related to cell preparation and three-dimensional cell culture technical field, specifically at to provide C liquid, D liquid and E liquid three kinds of gradient concentration working solution.When dissociating, the mesenchymal stem cell ball to be dissociated is determined its average particle size;According to the average particle size, select C liquid, D liquid or E liquid as digestive juice as described above;The selected digestive juice is mixed with the mesenchymal stem cell ball, and digestion reaction is carried out under oscillation condition, and single cell suspension is obtained.The application establishes the hierarchical matching rule of mesenchymal stem cell ball particle size and specific digestive juice concentration, and optimizes oscillation digestion condition, so as to maintain high activity rate and complete phenotype characteristics while efficiently dissociating, realizes the standardization of process and the controllable of dissociation quality, provides stable and reliable solution for the large-scale preparation and quality control of mesenchymal stem cell ball.
Owner:JINJU BIOPHARMACEUTICAL (NANJING) CO LTD

A method for efficiently preparing mouse primary hepatocytes

PendingCN122427858ACell-Extracellular MatrixLiver parenchyma
The present application relates to cell preparation process in the field of bioengineering, disclose a kind of high-efficiency preparation mouse primary hepatocyte method, comprising: perfusion needle is placed in reverse direction to liver direction through inferior vena cava, utilize the blood vessel clamp with elastic micro-tooth damping surface to lock the perfusion needle and inferior vena cava wall;The overflow damping of hepatic portal vein outflow end is dynamically regulated by blood vessel clamp, to construct controlled positive pressure permeation environment in liver vascular bed;Collagenase solution is radially permeated to liver parenchyma using the environment, the present application makes enzyme solution radial deflection and cross blood sinus barrier into di's cavity by constructing micro-pressure environment, eliminate the digestion dead zone originally due to flow path preference, ensure that enzyme molecule and extracellular matrix are uniformly contacted in global dimension, realize the collaborative promotion of tissue cutting efficiency and total amount of cell release, ensure that the output cell population has highly consistent physiological state.
Owner:SHANGHAI HEYOUSHENG BIOTECHNOLOGY CO LTD

Method for preparing nk cells to reverse tumor microenvironment inhibitory signals and applications thereof

The application provides an immune cell preparation method capable of reversing tumor microenvironment immunosuppression signals and application thereof. The method for reversing the inhibitory signals is to replace the intracellular segment of a TIGIT receptor with a 4-1BB costimulatory domain and an IL-18R and a CD3 intracellular segment, and the immune cells expressing the chimeric antigen receptor recognize CD155 in a tumor microenvironment through TIGIT, avoid loss of function or exhaustion of the immune cells, and stimulate the immune cells to exert stronger tumor killing activity.
Owner:SHANGHAI NK CELLTECH CO LTD

Method for enhancing urea circulation function of 3D bio-printed liver organ

The invention relates to the technical field of biological engineering, and discloses a method for enhancing the urea circulation function of a 3D biological printing liver organ, which comprises the following steps: a cell preparation step: through a genetic engineering technology, enabling mitochondria of liver cells to express a light-driven proton pump, and enabling intracellular expression of a fluorescent probe for reporting ammonia concentration; an organ construction step: mixing the cells with hydrogel to prepare bio-ink, and constructing a three-dimensional liver organ by using a 3D biological printing technology; a system integration step: placing the liver organ in an integrated photoelectric regulation and control system capable of monitoring a fluorescence signal in real time and applying illumination; and a closed-loop enhancement step: the system monitors the ammonia concentration in real time, and when the ammonia concentration exceeds a preset threshold value, illumination is automatically applied to activate the proton pump, so that the urea circulation function is enhanced as required. Through closed-loop light control and a subcellular organelle energy channel, on-demand, efficient and high-temporal-spatial-resolution dynamic enhancement of the liver organ urea circulation function is achieved.
Owner:BEIJING JINGZHUN BIOTECHNOLOGY CO LTD

Exploring method, cell preparation method, cell sorting method, cell, and cell product manufacturing method

PendingCN122295458ACricetulusChinese hamster
One approach to the exploration method includes calculating a TAD score representing transcriptional activity for each TAD present in the genome and selecting TADs based on the TAD scores. Another approach includes analyzing a genome obtained from cells expressing a foreign gene and selecting the boundaries of regions containing the foreign gene. Cell preparation involves inserting the target gene into the regions identified by the exploration method. Cell sorting involves calculating a TAD score representing transcriptional activity for each TAD present in the genome, selecting TADs based on the TAD scores, and selecting cells containing the target gene in the selected TADs. The cells are derived from Chinese hamsters and are cells in which the target gene has been inserted into at least one specified region. The method for manufacturing the cell product includes culturing the cells to express the target gene.
Owner:FUJIFILM CORP

Cell composition for promoting hair regeneration and application thereof

The invention discloses a cell composition for promoting hair regeneration and application thereof, and belongs to the field of biological medicine, the active component of the cell composition comprises a mixture of hair papilla cells, keratinocytes and stem cell growth factors; wherein the number ratio of the hair papilla cells to the keratinocytes is (1-3): (1-3), and the stem cell growth factor mixture comprises bFGF, KGF, HGF, VEGF, TGF-beta1 and PDGF. The cell preparation disclosed by the invention can be used for improving the hair growth effectiveness and solving different types of alopecia symptoms except for androgen alopecia, has a remarkable treatment effect on androgen alopecia, and has a remarkable effect on treating various alopecia symptoms. The traditional Chinese medicine composition has treatment effects on alopecia areata, alopecia in a resting period, alopecia in a growing period, scar alopecia and androgen-derived alopecia.
Owner:BEIJING XINGHUI REGENERATION TECH CO LTD

Transverse distance adjusting mechanism for gun heads of cell slide-making dyeing machine

The utility model relates to the technical field of cell slide dyeing, and discloses a gun head transverse spacing adjusting mechanism of a cell slide dyeing machine, which comprises a guide rail, the inner wall of the guide rail is in threaded connection with a screw rod, the right side of the guide rail is fixedly connected with a positioning frame, and the right end of the screw rod is fixedly connected with a fixed disc I; a second fixing disc is fixedly connected to the right side of the first fixing disc, a plurality of transmission blocks are fixedly connected to the outer wall of the second fixing disc, an inner gear ring is slidably connected to the inner wall of the positioning frame, a sun gear is arranged on the inner wall of the inner gear ring, and a plurality of planetary gears are connected to the outer wall of the sun gear in a meshed mode. And the left side of the planetary gear is fixedly connected with a planetary carrier. According to the utility model, the telescopic rod is started to push the asynchronous motor to slide in the positioning frame, and the asynchronous motor is clamped with the transmission groove in the transmission disc on the outer wall of the transmission shaft II, so that the purpose of adjusting the fineness while transversely moving is achieved, the tablet production precision is improved, and the practicability is improved.
Owner:XIAOGANG HONGXIANG BIOLOGICAL TECH CO LTD

Manufacturing method of thin film solar cell with low warping degree

The invention discloses a low-warping-degree thin film solar cell manufacturing method, and aims to solve the warping problem caused by mismatching of material intrinsic stress and thermal expansion coefficient in cell preparation. According to the technical scheme, when an epitaxial wafer is preprocessed, an Au contact layer, a compressive / tensile stress metal alternating film layer and an Au bonding layer are sequentially evaporated; when the thin film supporting substrate is pretreated, a Cr-Ti-Pt-Au bonding layer is evaporated on one side, a silicon nitride, silicon oxide or aluminum oxide warping adjustment layer is prepared on the other side, and photoetching trepanning and evaporation of a back contact electrode are required to be performed on the warping adjustment layer for an electrode structure on the different side; and after contraposition permanent bonding, a finished product is obtained through temporary bonding, substrate removal, photoetching coating, antireflection film evaporation and alloy treatment. Internal and external stress is offset through the double-layer warping adjusting structure, the same-side electrode structure and the different-side electrode structure are adapted, the process is mature and easy to popularize, the warping degree of the battery is effectively reduced, and the product yield and the use reliability are remarkably improved.
Owner:XIAMEN YINKE QIRUI SEMICON TECH CO LTD

Synergistic engineering tumor infiltrating lymphocyte and preparation method thereof

The invention provides a synergistic engineering tumor infiltration lymphocyte and a preparation method thereof, and belongs to the technical field of biological medicines. The engineering tumor infiltrating lymphocyte capable of simultaneously expressing the activated fusion protein and the retention fusion protein is constructed, so that the recognition and killing capabilities of the engineering tumor infiltrating lymphocyte on tumor cells are remarkably improved, and meanwhile, the self-killing of the cells in an activated state is effectively avoided, so that the continuity and the safety of treatment are enhanced. The cell preparation shows a good application prospect in solid tumor treatment, and a new effective strategy is provided for overcoming the limitation of the existing immune cell therapy.
Owner:ZHEJIANG YUANQI FUTURE BIOTECHNOLOGY CO LTD

IBC photovoltaic cell and preparation method thereof

The invention discloses an IBC photovoltaic cell and a preparation method thereof, and relates to the technical field of cell preparation, the IBC photovoltaic cell comprises a silicon substrate, the back surface of the silicon substrate is provided with a plurality of back surface first doped regions, back surface second doped regions and isolation groove regions which are alternately arranged, the isolation groove regions are groove isolation G regions, and the groove isolation G regions are arranged on the back surface of the silicon substrate. The back metallization circuit pattern of the IBC photovoltaic cell adopts an 0BB connection circuit pattern without a main grid connection line. According to the invention, an 0BB electrocoppering line without a main grid connecting line is adopted, so that a large number of trench isolation G regions do not need to be arranged at the positions of the patterned main grids I and II, the processing flow is simplified, and a cylindrical contact channel is formed through processes such as laser film opening and acid cleaning. The aluminum-titanium alloy barrier layer, the copper seed layer, the copper conductive layer and the antioxidant tin layer are adopted to construct the metal electrode, so that the non-silicon cost of the IBC photovoltaic cell is further reduced, meanwhile, the contact resistance between aluminum and polycrystalline silicon is lower, and compared with a silver-coated copper wire screen printing process, the electrical property of the cell is optimized.
Owner:YIBIN YINGFA DERUI TECHNOLOGY CO LTD

A method for photochemical treatment of riboflavin based on multi-wavelength LED and its application in treatment of graft-versus-host disease

PendingCN122424330AApoptosisPlatelets blood
The present application belongs to the field of photochemical therapy, and particularly relates to a method for photochemical treatment of riboflavin based on multi-wavelength LED and application thereof in treatment of graft-versus-host disease. The present application selects different wavelength LED combinations as light sources to optimize the riboflavin photochemical method, controls the light intensity at 1000-25000 μW / cm², controls the irradiation time at 5-15 minutes, and controls the riboflavin concentration at 20-150 μM, thereby significantly improving the photochemical reaction effect. The method can efficiently induce PBMC apoptosis in a plasma environment, significantly reduces the levels of key pro-inflammatory cytokines such as TNF-α, IL-6 and IFN-γ, and has no significant influence on platelets, hemoglobin and blood clotting function, thereby providing a safe and controllable new cell preparation method for GVHD treatment.
Owner:BEIJING DAYOU TIANHONG TECH CO LTD

A method for preparing plant single cells

The present application adopts the way of plant explant callus culture, adds carbon source containing fluorescent group in the culture medium, induces callus with fluorescent group, thin cell wall, easy to be single enzymolysis, uniform cell morphology and size, the cell after single enzymolysis is detected by flow cytometry, the purity and activity of the prepared leaf single cell are 98.5% and 99.95 respectively, the agglomeration rate is only 1.5%, the cell purity and activity are increased by 32.6% and 21.05 respectively compared with the traditional technical scheme, and the agglomeration rate is reduced by 31.0%; the rice seed is used as the explant, and the seed single cell with cell purity and activity of 99.5% and 100% is obtained, which breaks through the limitation of traditional enzymolysis method that cannot prepare seed single cell; in addition, through the common induction of safflower leaf and rice seed, the cell purity and activity prepared are as high as 99.98% and 100.0, which overcomes the limitation of single cell preparation caused by interspecific heterogeneity.
Owner:BEIJING EPSILON BIOTECHNOLOGY CO LTD

Chondrogenic stem cell for promoting joint repair and use thereof

Provided are a chondrogenic stem cell for promoting joint repair and use thereof. Specifically, disclosed is a cell preparation used for alleviating or treating osteoarthritis, comprising a Procr-positive pluripotent stem cell. The Procr-positive chondrogenic stem cell can differentiate and generate downstream chondrocytes in vivo and promote cartilage repair in osteoarthritis damage, which will be a more effective cell treatment method. In addition, Procr proteins can be used as a new target of a drug for treating osteoarthritis.
Owner:TONGJI UNIV

Environmental conditioning system and cell sheet making machine

The utility model discloses an environment conditioning system and a cell pelleter. The environment adjusting system comprises a machine body, a film making machine and a film making machine, an air inlet of the suction pump is communicated with the flaking cavity; the atomization box is used for storing fluid, and an air inlet of the atomization box is communicated with an air outlet of the suction pump; the atomizer is arranged in the atomization box and used for atomizing fluid in the atomization box; and the air outlet pipe is used for communicating the tabletting cavity with the air outlet of the atomization box and is used for conveying the atomized fluid to the tabletting cavity. According to the application, the suction pump is used for pumping air in the tablet preparation cavity into the atomization box, the atomizer is used for atomizing fluid in the atomization box so as to increase the humidity in the air, and then the humidified air is conveyed to the tablet preparation cavity through the air outlet pipe, so that the tablet preparation cavity is humidified in an internal circulation manner; air pollution of the external environment to the flaking cavity is prevented, and the stability of the flaking environment is favorably maintained.
Owner:GUANGZHOU DAHUI BIOTECH CO LTD +1

A cell preparation targeting bone metastatic tumor cells, its preparation method and application

This invention discloses a cell preparation targeting bone metastatic tumor cells, its preparation method, and its application, belonging to the field of biomedical technology. The cell preparation comprises senescent neutrophils and drug-loaded cationic liposomes internalized within them. The cationic liposomes encapsulate a STING agonist and a pan-photokinase inhibitor. The preparation method includes: preparing drug-loaded liposomes using a thin-film dispersion-ultrasound method; obtaining senescent neutrophils through in vitro culture of extracted neutrophils; and preparing a senescent neutrophil preparation loaded with liposomes. This invention utilizes the natural bone marrow homing ability of senescent neutrophils to achieve precise targeting of bone metastatic tumor cells, and co-delivers two drugs through liposomes, simultaneously activating anti-tumor immunity and inducing tumor cell apoptosis / autophagy, synergistically treating bone metastases. This system has high drug loading capacity, good encapsulation efficiency, and sustained-release effect, and the preparation process is simple and controllable, providing a new strategy for the treatment of cancer bone metastases.
Owner:SICHUAN UNIV

Novel TOPCon battery preparation process and device

The invention discloses a novel TOPCon battery preparation process and device, and relates to the technical field of battery processing, and the novel TOPCon battery preparation process comprises the following steps: S1, preparing a sheet; s2, scribing: segmenting the whole cell by using laser; s3, side face polishing is conducted, specifically, chain type etching and alkali washing are conducted on the cutting face, and the cutting face is corroded at the temperature of 30-60 DEG C through 10-30% of TMAH and 5-20% of IPA aqueous solution; s4, edge passivation is conducted, specifically, an aluminum oxide passivation film of 20-50 nm is deposited in an atomic layer deposition mode, and annealing is conducted for 30-60 min at the temperature of 250-300 DEG C; and S5, IV testing: testing the appearance, IV and EL performance of the battery piece, and then grading. Preferably, the flaking process comprises the steps of texturing and boron diffusion. According to the invention, a battery panel is driven to move in a manner of carrying liquid through a roller, and silicon, silicon nitride and silicon oxide on a cut surface of the battery can be corroded and polished by using a TMAH + IPA solution and an HF solution.
Owner:ANHUI XUHE NEW ENERGY TECH CO LTD

Sample loading system for synchronous thermal analysis of photovoltaic cell silver paste

The utility model relates to the field of material thermal analysis, in particular to a sample loading system for synchronous thermal analysis of photovoltaic cell silver paste, which comprises a partition module and a blowing module, the partition module is of a cylindrical structure with openings in the two ends, the partition module is arranged in the crucible, and photovoltaic cell silver paste to be analyzed enters the crucible through the openings in the two ends of the partition module; and the blowing module is used for flattening the photovoltaic cell silver paste to be analyzed in the crucible in a blowing manner. According to the utility model, the blocking module is used for preventing the side wall of the crucible from being accidentally stained when STA analysis sample loading is carried out on the photovoltaic cell silver paste. And the blowing module sprays gas with proper force, so that the silver paste is uniformly distributed in the accommodating body and flatly laid at the bottom of the crucible. Further, the temperature difference of different parts of the sample in test analysis is reduced, more accurate thermal reaction temperature is obtained, the consistency of thermal analysis curve shapes is improved, and the research of photovoltaic cell silver paste sintering and photovoltaic cell preparation processes is effectively supported.
Owner:CHINA ELECTRONICS STANDARDIZATION INST

Back contact battery preparation method adopting laser-induced crystallization process and battery

The invention belongs to the technical field of back contact cells, and particularly relates to a back contact cell preparation method adopting a laser-induced crystallization process and a cell, and the method comprises the steps: S1082, carrying out the laser engraving of at least a part of a second doped amorphous silicon layer in a second semiconductor opening region through the laser-induced crystallization process, crystallizing the second doped amorphous silicon layer corresponding to the laser engraving area to form a second doped microcrystalline silicon layer; the conditions of the laser-induced crystallization process are as follows: the laser spot energy density is less than 190mJ / cm < 2 >, and the spot overlapping rate is greater than or equal to 50%. According to the invention, the requirement on the thickness of amorphous silicon and the requirement on the consistency of a film layer are greatly reduced; meanwhile, the requirements on a laser and a light path are reduced, and the preparation process is simplified, so that large-scale mass production is facilitated; and meanwhile, good battery efficiency is considered, and the amorphous silicon productivity is improved.
Owner:GOLDEN SOLAR (QUANZHOU) NEW ENERGY TECH CO LTD

Magnetic bead sorting NK immune cell preparation and extraction device

The utility model provides a magnetic bead sorting NK immune cell preparation and extraction device which comprises a base, a control shell is upwards arranged on the base, a magnetic field shell is arranged in the middle of the front side of the control shell, and a plurality of magnetic field grooves are inwards formed in the front side of the magnetic field shell. Each magnetic field groove comprises a first-level magnetic field groove located on the upper portion and a second-level magnetic field groove located on the lower portion, the section of each first-level magnetic field groove is larger than that of the corresponding second-level magnetic field groove, and a first-level magnetic field electromagnet is arranged on the periphery of each first-level magnetic field groove in the magnetic field shell. A secondary magnetic field electromagnet is arranged on the periphery of each secondary magnetic field groove in the magnetic field shell; the chromatographic column is divided into a sample section, a first-stage chromatographic section matched with the first-stage magnetic field groove and a second-stage chromatographic section matched with the second-stage magnetic field groove from top to bottom. The utility model provides a grading magnetic bead sorting structure, which can effectively avoid the blocking phenomenon, effectively improve the sorting efficiency of cells and improve the final yield.
Owner:AOCHEN BIOLOGICAL (YUNNAN) CO LTD

Liquid dispenser

This utility model discloses a liquid extractor, belonging to the field of cell preparation technology, comprising: a needle, a liquid guide tube, and an exhaust tube. The needle has an inlet hole at its head and is used to penetrate into a liquid storage container. The needle also has an outlet hole and an exhaust hole communicating with the inlet hole. The liquid storage container is located above the needle. The liquid guide tube communicates with the outlet hole, and the end of the liquid guide tube away from the needle is adapted to selectively connect to a plug or a cell preparation device. The exhaust tube communicates with the exhaust hole. According to this utility model, the liquid extractor can be used to continuously inject physiological saline into a cell preparation device and can prevent physiological saline leakage.
Owner:WUHAN TIANSAI CELL BIOTECHNOLOGY CO LTD

A cell line capable of inhibiting the replication of african swine fever virus and a construction method thereof

This invention discloses a cell line capable of inhibiting African swine fever virus (ASFV) replication and its construction method, relating to the field of biotechnology. The construction method includes the following steps: knocking out the ANXA1 gene in African green monkey kidney cells to construct a stably inherited ANXA1 gene knockout cell line; this ANXA1 gene knockout cell line is the cell line capable of inhibiting ASFV replication. The cell line provided by this invention can significantly inhibit ASFV replication. Compared to existing porcine cell lines, this cell line has a clear background, is free from endogenous porcine pathogen contamination, and can be passaged indefinitely, solving the problems of cumbersome porcine cell preparation and large batch-to-batch variations. Simultaneously, this cell line provides a targeted cell model for studying the replication mechanism of ASFV and establishes an efficient evaluation system for high-throughput screening of anti-ASFV drugs, thereby facilitating the development of research related to ASFV prevention and control.
Owner:SHENZHEN INST OF GUANGDONG OCEAN UNIV

Machine learning-based cell preparation data anomaly analysis method and system

The application discloses a kind of cell preparation data abnormality analysis method and system based on machine learning, it is related to data abnormality analysis field, the present application is by setting feature and abnormal type first, then constructs two kinds of mapping model, finally combines real-time acquisition data to complete abnormality determination and hierarchical processing, can distinguish different abnormal type and match corresponding measure automatically, greatly improve the standardization and automation degree of abnormal processing;In addition, by combining the judgment of cell state and storage operation characteristics, and further distinguishing whether the cell data storage abnormality is induced by the cell state for the comprehensive abnormality, respectively using the priority repair or parallel repair strategy, from the root, solve the abnormality transmission and coupling problem that traditional method cannot handle, both avoid the misjudgment of single threshold judgment, and solve the difficult problem that complex abnormality is difficult to trace and effectively eradicate, ensure that cell preparation is stable and data quality is reliable.
Owner:ANHUI YIWENSAI BIOTECHNOLOGY CO LTD