384 results about "Cell preparation" patented technology

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.

The invention relates to a photovoltaic cell preparation method, a photovoltaic cell, a laminated cell and a photovoltaic cell module. Providing an initial battery piece; depositing and forming a protective layer on the surface; carrying out laser treatment twice; printing and sintering to form an electrode; and carrying out at least two times of laser-assisted sintering on the electrode. According to the photovoltaic cell preparation method, the protection layer at the first area is removed, the doping concentration at the first area is improved, and at least two times of laser-assisted sintering are combined, so that on one hand, the metal contact area is optimized, the usage amount of silver paste is reduced, the material cost is reduced, and meanwhile, paste contact sintering is enhanced; damage caused by laser grooving doping is removed; and on the other hand, the doping concentration is optimized, the contact resistance is remarkably reduced, the open-circuit voltage and the filling factor are favorably improved, the shading loss is reduced, and the current collection efficiency is ensured, so that the photoelectric conversion efficiency and the reliability of the cell are remarkably improved, the production process is simplified, and the production cost is reduced.

The invention discloses a preparation method of a solar cell and the solar cell. The preparation method comprises the following steps: removing a second mask layer on a first surface and a side surface of a silicon substrate by adopting a chained acid etching process; removing the second tunneling passivation structures on the first surface and the side surface of the silicon substrate by adopting a chained alkali etching process; and removing the second tunneling passivation structures in the first region and the third region of the second surface of the silicon substrate by adopting a groove type etching process, and forming a suede light trapping structure on the third region of the second surface of the silicon substrate and the first surface, and removing the first mask layer in the first area and the second mask layer in the second area on the second surface of the silicon substrate. According to the preparation method of the solar cell and the solar cell, the cell preparation process can be optimized, the environment is protected, the texture surface quality is improved, and the cell efficiency is improved.

The present invention relates to the technical field of biopharmaceutical apparatuses, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator, and a cell culture device; the cell preparation device comprises a preparation chamber, wherein a first sealing valve is arranged on a side wall of the preparation chamber; the incubator has a culture cavity, wherein a second sealing valve is arranged on a side wall of the culture cavity, and the second sealing valve is configured to work with the first sealing valve such that the incubator is hermetically docked with or separated from the cell preparation device; and the cell culture device is used for accommodating the incubator, supplying power and a gas to the incubator, and controlling a cell growth environment in the incubator. Because cells grow in a sterile environment all the time and the pollution risk is relatively low, no frequent sterilization is required. Normal growth of cells is avoided from being affected by frequent sterilization, and the culture time is saved. Therefore, the cell preparation and culture efficiency is relatively high.

The invention relates to the field of photovoltaic cell preparation, and particularly provides a formula adjusting method and device for a plasma enhanced chemical vapor deposition process. The method comprises the following steps: acquiring a state parameter vector of a plasma enhanced chemical vapor deposition process machine in an operation process, and predicting a predicted film layer thickness corresponding to the state parameter vector; if the predicted film layer thickness does not meet the preset error, selecting k adjustable factors with top contribution degrees in the state parameter vectors as target adjustment factors; according to the gradient descending direction of the film layer thickness, searching a historical state parameter with the minimum Euclidean distance with the state parameter of each target regulation factor in a good product historical database, and taking the historical state parameter as a current formula parameter; and according to the state parameter vector corresponding to the current formula parameter, regenerating a predicted film layer thickness until the predicted film layer thickness meets a preset error, and taking the parameter in the current state parameter vector as a target formula. According to the technical scheme provided by the invention, the reject ratio of photovoltaic cell production is reduced.

The invention relates to the technical field of lithium batteries, in particular to a preparation method of a high-temperature-resistant lithium battery cell. The invention discloses a preparation method of a high-temperature-resistant lithium battery cell, which is characterized in that through full-chain synergy of modification of positive and negative electrode materials, electrolyte optimization and structural design, a positive electrode adopts nickel cobalt lithium aluminate with a core-shell structure, and a metal organic framework coordination layer, polyaniline-polyacrylic acid multilayer coating and foam aluminum foil 3D printing coating are combined. The negative electrode takes silicon-carbon core-shell particles as a substrate, and breaks through the expansion bottleneck of a silicon-based negative electrode through metal organic framework confinement coating, polyaniline-polyacrylic acid cross-linked network modification and foam copper foil functionalization. The electrolyte adopts a film-forming additive and lithium difluorophosphate to form a stable SEI film, and internal resistance increase at high temperature is inhibited. The aramid fiber coating diaphragm and the aluminum alloy shell are matched, so that the thermal stability of the battery cell is remarkably improved, the battery cell can stably work in a wide temperature range environment, high energy density and long cycle life are comprehensively realized, and key support is provided for a long-endurance and high-safety battery.

The invention relates to a perovskite / crystalline silicon laminated solar cell structure taking a tin dioxide thin film as a composite layer and a preparation method of the perovskite / crystalline silicon laminated solar cell structure, and belongs to the technical field of photovoltaics. According to the perovskite / crystalline silicon laminated solar cell structure, the tin dioxide thin film is used as a composite layer, so that the cost of cell preparation is reduced; in the preparation method of the perovskite / crystalline silicon laminated solar cell, the composite layer and the electron transport layer can adopt the same preparation method, so that the equipment cost in the preparation process is reduced; the perovskite / crystalline silicon laminated solar cell obtained by the cell structure and the preparation method provided by the invention is relatively low in preparation cost and relatively high in photoelectric conversion efficiency.

According to the method, a plant explant callus culture mode is adopted, a carbon source containing a fluorophore is added into a culture medium, the callus which is provided with the fluorophore, thin in cell wall, easy to dissociate by a single enzyme and uniform in cell shape and size is generated through induction, and the cell dissociated by the single enzyme is detected by a flow cytometry, so that the cell quality is improved. The single cell purity and activity of the prepared leaf are 98.5% and 99.95% respectively, the clotting rate is only 1.5%, the cell purity and activity are improved by 32.6% and 21.05% respectively compared with those of a traditional technical scheme, and the clotting rate is reduced by 31.0%; according to the method, rice seeds are used as explants, seed single cells with the cell purity and activity being up to 99.5% and 100% respectively are obtained, and the limitation that the seed single cells cannot be prepared by a traditional enzymolysis method is broken through; besides, through co-induction of the safflower leaves and the rice seeds, the purity and activity of the prepared cells are as high as 99.98% and 100.0 respectively, and the limitation of single cell preparation caused by heterogeneity among species is overcome.

The invention discloses a TopCon cell and a TopCon cell preparation method, and belongs to the field of solar cells. The structure of the passivation layer is optimized, the doped polycrystalline silicon layer is reserved in the metal contact area, the doped polycrystalline silicon layer and the substrate form a high-low junction to provide field passivation, and ohmic contact is formed between the doped polycrystalline silicon layer and the electrode; a doped polycrystalline silicon carbide layer is arranged in a non-metal contact area, and the doped polycrystalline silicon carbide layer and a substrate form a heterojunction to provide field passivation and serve as a carrier transverse transmission layer. The parasitic absorption of the passivation layer is reduced by removing the doped polycrystalline silicon layer in the non-metal contact region. The light absorption coefficient of the doped polycrystalline silicon carbide layer is low and the doped polycrystalline silicon carbide layer is thin, so that parasitic absorption can be reduced. Ohmic contact is formed between the doped polycrystalline silicon layer in the reserved metal contact area and the electrode, so that low recombination loss can be ensured when the doped polycrystalline silicon layer is in contact with the metal grid line. Field passivation is formed in the metal contact area and the non-metal area, and the passivation effect of the whole surface of the substrate is guaranteed.

A photovoltaic cell string, a photovoltaic cell panel and a photovoltaic cell module, which are applied to the field of photovoltaic cell preparation. The cell string comprises: a plurality of cells, welding strips, which are arranged on first surfaces and second surfaces of the cells, and protective films, which are arranged on outer sides of the welding strips, wherein there is an overlapping region between adjacent cells among the plurality of cells; and the protective films located on preset cell surfaces of the adjacent cells extend to the overlapping region, such that by using the protective films, the stress generated between the cells and the welding strips in the overlapping region is reduced; the preset cell surfaces are the surfaces of the sides of the adjacent cells where the cells are stacked on each other; and the adjacent cells form an electrical connection by means of the welding strips located on the preset cell surfaces. In the present application, the protective films located on the preset surfaces of the cells in the adjacent cells extend to the overlapping region, such that the stress generated between the cells and the welding strip can be reduced.

The present invention relates to the technical field of solar cell preparation, and specifically provides a heterojunction cell preparation method and a heterojunction cell. The heterojunction cell preparation method comprises: providing a semiconductor substrate layer, at least one side of the semiconductor substrate layer being provided with a damage layer; performing first texturing treatment on the semiconductor substrate layer to remove some damage from the damage layer, such that the surface of the side of the semiconductor substrate layer having the damage layer forms an inverted pyramid textured surface, the inverted pyramid textured surface comprising a plurality of first microstructural units; and, after the first texturing treatment, performing second texturing treatment on the side of the semiconductor substrate layer where the inverted pyramid textured surface is formed, so as to form a plurality of second pyramids on the surfaces of the first microstructural units of the inverted pyramid textured surface, and remove remaining damage from the damage layer. The heterojunction cell preparation method of the present invention enables prepared cells to have high fill factors at low costs.

The invention relates to the technical field of biopharmaceutical equipment, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator and a cell culture device, the cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber; the incubator is provided with a culture chamber, a second sealing valve is arranged on the side wall of the culture chamber, and the second sealing valve is used for being matched with the first sealing valve, so that the incubator and the cell preparation device are in sealed butt joint or sealed separation; the cell culture device is used for accommodating the culture box, supplying power and gas to the culture box and controlling the cell growth environment in the culture box. As the cells always grow in the sterile environment, the pollution risk is low, frequent sterilization is not needed, the normal growth of the cells is prevented from being influenced by frequent sterilization, the culture time is saved, and the cell preparation and culture efficiency is high.

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.

The invention discloses an IBC photovoltaic cell, and relates to the technical field of cell preparation, and the IBC photovoltaic cell comprises a silicon substrate, the back surface of the silicon substrate is provided with a first doped region, a first isolation groove G1, a second doped region and a second isolation groove G2 along the forward direction of an X axis, a first doped region, a third isolation groove G3, a second doped region and a fourth isolation groove G4 are arranged on the back face of the silicon substrate in the reverse direction of the Z axis, the first isolation groove G1 and the second isolation groove G2 are both perpendicular to the main grid, the width of the first isolation groove G1 and the width of the second isolation groove G2 are both 100-200 micrometers, the third isolation groove G3 and the width of the fourth isolation groove G4 are both parallel to the main grid, and the width of the third isolation groove G3 and the width of the fourth isolation groove G4 are both 120-320 micrometers. According to the invention, the width of the isolation groove is widened through the laser film opening parameter, so that when pollutants are attached to the groove, the conduction risk can be reduced, the production yield of the battery can be improved, and the widths of the first doped region and the second doped region are narrowed due to the widened width of the isolation groove, thereby weakening the parasitic absorption of the back surface and improving the performance of the battery. And the double-sided rate and the generating capacity of the centralized assembly are improved.

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.

The invention provides application of apoptotic cell pretreated macrophages in preparation of an osteoarthritis treatment preparation, a cell preparation and a preparation method, and relates to the technical field of biomedicine.In the embodiment of the invention, based on a mouse development model and an osteoarthritis (OA) model, it is found that in the area away from the cartilage surface, the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment, and the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment in the area away from the cartilage surface; in the macrophages, the content of the interment-related LAMP1 protein is obviously higher than that of the macrophages close to the cartilage surface area, and the expression results of TGF-beta 1 and LRG1 are respectively opposite and consistent with those of the LAMP1. The invention also finds that after the chemotactic effect of apoptotic cells on macrophages is weakened, the cellular burial effect is limited, and the cartilage matrix development disorder is caused. Based on development process inspiration, the invention provides a treatment strategy: bone marrow-derived macrophages pretreated by apoptotic cells are injected into articular cavities, and a good cartilage regeneration treatment effect can be achieved in a mouse arthritis model. In addition, the bone marrow-derived macrophages pretreated by apoptotic cells can still express TGF-beta1 after being injected into the body for 3 days, so that the effect of continuously promoting cartilage regeneration is achieved.

There are provided measures for enabling / realizing efficient / favorable data forwarding in lower-layer mobility in / for a mobile communication system, namely a radio access network of a mobile communication system, such as L1 / L2 centric mobility. Such measures exemplarily comprise that a lower-layer entity in a radio access network of a mobile communication system obtains a configuration of lower-layer mobility and data forwarding in lower-layer mobility from a higher-layer control plane entity, initiates data forwarding to at least one target cell of lower-layer mobility in accordance with the configuration, and notifies a higher-layer user plane entity of initiation of data forwarding, wherein the at least one target cell of lower-layer mobility is identified. The configuration may indicate on-time data forwarding, wherein data forwarding to a target cell is triggered when a serving cell change decision is completed, or ay indicate early data forwarding upon preparation of candidate target cells for lower-layer mobility, wherein data forwarding to at least one of one or more candidate target cells is triggered when candidate target cells preparation is completed.

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and / or resources and / or reduced handling compared with other methods.

The utility model discloses a vacuum filtration device, and relates to the technical field of cell preparation, the vacuum filtration device comprises a first funnel assembly, a second funnel assembly and a reciprocating vacuum pump, the first funnel assembly and the second funnel assembly respectively comprise a funnel body and a cover body, and the cover body detachably covers the funnel body; the funnel body of the first funnel assembly is detachably connected with the cover body of the second funnel assembly; the funnel body of the first funnel assembly and the funnel body of the second funnel assembly are detachably connected with the air inlet end of the reciprocating vacuum pump; the air outlet end of the reciprocating vacuum pump is detachably connected with the funnel body of the first funnel assembly; the problems of low filtering efficiency and inconvenience in cleaning are solved; the whole structure is convenient to disassemble, assemble and sterilize, potential problems caused by liquid suck-back in the filtering process are avoided, negative pressure is provided through the reciprocating vacuum pump, meanwhile, pressurization is conducted in the funnel body of the first funnel assembly, and the filtering efficiency is improved.

1. Name of the design product: packing box (cell preparation). 2. Use of the design product: the design product is used for packing and storing cell liquid. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The present application relates to the technical field of biological cell culture, and particularly relates to a three-dimensional cell culture device with automatic liquid replacement function, comprising: a box body; a rotating assembly, a liquid replacement assembly and a control display assembly are integrally installed in the box body. The three-dimensional cell culture device with automatic liquid replacement function provided by the present application integrates microgravity simulation and liquid replacement function in a relatively closed culture box body space, ensures that the culture environment in the cell culture process has high stability by not excessively affecting the air flow, temperature and humidity environment, etc. in the culture box body during liquid replacement; the device for replacing culture solution does not need to rotate, effectively releases the culture space while reducing the energy consumption required in the rotation process, can load multiple culture bottles for three-dimensional culture and automatic liquid replacement at the same time, and has high potential for large-scale cell preparation.

The utility model relates to the field of medical instruments, and discloses a centrifugal mechanism of a full-automatic liquid-based cell slide-making and dyeing all-in-one machine, which comprises a liquid-based cell slide-making and dyeing all-in-one machine body, and a top cover is hinged on the outer wall of the liquid-based cell slide-making and dyeing all-in-one machine body. The inner wall of the liquid-based cell slide preparation and dyeing all-in-one machine body is slidably connected with a sedimentation cabin, a test tube is placed at the top of the sedimentation cabin, the outer wall of a top cover is rotatably connected with a gear, the outer wall of the gear is fixedly connected with a cleaning rod, and the inner wall of the top cover is fixedly connected with a sealing ring plate; a rotating disc is slidably connected into the inner wall of the sealing ring plate. According to the cleaning device, the ultrasonic transducer at the bottom end of the cleaning rod is matched with physical cleaning of the cleaning rod, stubborn stains and cell residues on the inner wall and the bottom of a test tube can be efficiently removed, the cleaning effect is remarkably improved, it is guaranteed that a sample is not interfered by impurities in subsequent liquid-based cell preparation dyeing operation, and the detection accuracy is improved.

The application provides a kind of HLA-A24:02 APC cell preparation method and application, it is related to cell preparation technical field.The present application constructs the APC cell (K562-HLA-A24:02) that can replace HLA-A24:02 subtype DC cell, fills the blank of HLA-A24:02 subtype special engineering APC cell, and the cell can realize the antigen presentation function consistent with natural HLA-A24:02 subtype DC cell, provides special tool cell for the immune research for the HLA subtype;The present application constructs APC cell with K562 cell line as base, K562 cell can be in vitro permanent passage amplification, without repeatedly separating and inducing DC cell from human peripheral blood, reduces the use amount of peripheral blood, reduces the raw material dependence and cost of cell acquisition, while avoiding the influence of individual difference of peripheral blood source on experimental results.

The utility model discloses a cell transfer bag with a novel structure, which comprises a transfer bag which is respectively connected with an input port, a liquid inlet conduit and an output port, and is characterized by further comprising a sampling bag which is communicated with the transfer bag through a plastic hose and is a closed bag body. The cell preparation sampling device is novel in structure and high in practicability, the upper end of the transfer bag is connected with the sampling bag through the plastic hose, when a cell preparation needs to be sampled, 1-2 ml of cell suspension is manually squeezed into the sampling bag, then the plastic hose between the sampling bag and the transfer bag is subjected to heat sealing, after heat sealing is completed, the sampling bag is taken down, and then cell activity and cell quantity detection can be conducted; the sampling is convenient and sterile.

The invention discloses a high-precision detection method of abnormal plasma cells, which comprises the following steps: preparing a film, immediately and uniformly mixing the extracted marrow fluid with an anticoagulant, sequentially diluting and centrifuging the marrow, then collecting a leukocyte layer, washing with a fluid medium RPMI 1640 / 5% FBS for at least one time, then counting the cells, adjusting the cell concentration to 1 * 10 < 6 > / ml, adding 100 [mu] l of the cell suspension into a funnel of a cell centrifugal smear device, centrifuging at room temperature for 1000 revolutions, continuing for 5 minutes, and preserving a slide filled with the cell suspension; the preparation of FISH cells comprises the following steps: firstly, preparing and pretreating a 90% formamide / 2 * SSC solution; according to the method, the accuracy and the efficiency of abnormal plasma cell detection are improved by combining an intelligent AI recognition model and a fluorescence in situ hybridization (FISH) technology. The application of the intelligent AI recognition model can reduce personal errors and improve the recognition capability of fluorescence signals, thereby improving the accuracy of detection results.

The invention relates to the technical field of biomedical treatment, particularly provides a cell preparation and subpackaging method, and aims to solve the problem that an existing cell preparation and subpackaging method is easy to cause cell burn. Specifically, the cell preparation and subpackaging method comprises the following steps: subpackaging cell sap into a plurality of cryopreservation bags; and injecting a cryopreservation solution into the cryopreservation bag, and in the process of injecting the cryopreservation solution, enabling the cryopreservation bag to reciprocate, so that the cell sap in the cryopreservation bag is uniformly mixed with the cryopreservation solution. According to the cryopreservation device, the cell sap can be subpackaged into the multiple cryopreservation bags, then the cryopreservation liquid is injected into each cryopreservation bag, and due to the fact that the number of the cell sap in each cryopreservation bag is small, heat generated when the cryopreservation liquid is injected is small, cell burn can be avoided, and the cell viability is improved.

The invention provides an NKT cell preparation as well as a preparation method and application thereof, and belongs to the field of biomedical treatment. The NKT cell preparation provided by the invention comprises NKT cells, a compound electrolyte injection, 5-10% of human serum albumin, 5-10% of raffinose and 2-5% of lysine, wherein the concentration of the NKT cells in the cell preparation is 1.0 * 10 < 6 >-1.0 * 10 < 8 > / mL; the prepared finished product preparation is long in shelf life, can be stably stored at 4 DEG C for more than 144 hours, and can be stably stored at-80 DEG C for at least 12 months.

The utility model relates to the technical field of cell slide, and discloses a gun head locking mechanism of a cell slide dyeing machine, which comprises a dyeing machine box, the top of the dyeing machine box is in sliding connection with a liquid storage pipe, the bottom of the liquid storage pipe is fixedly connected with a gun head shell, a vacuum generating device is arranged in the gun head shell, and the gun head shell is fixedly connected with the liquid storage pipe. The rear side of the vacuum generating device communicates with an adsorption cavity, a sealing column is fixedly connected to the bottom of the vacuum generating device, a plurality of air flow channels are formed in the top of the sealing column, a connecting pipe is fixedly connected to the middle of the sealing column, and the top of the connecting pipe communicates with the bottom of the liquid storage pipe. According to the pipette fixing device, the pipette is preliminarily clamped from the bottom of the connecting pipe through the lantern ring, suction force is generated through the vacuum generating device, the fixing effect is achieved, excessive mechanical extrusion and damage to the pipette cannot be caused by the fixing mode, the service life can be prolonged, and meanwhile it is avoided that the experiment result is affected by liquid pollution.