Low-frequency pulse electromagnetic field carbon dioxide constant-temperature cell incubator

Abstract

The invention belongs to the technical field of cell culture, and discloses a low-frequency pulse electromagnetic field carbon dioxide constant-temperature cell culture box which comprises a culture box body, a Helmholtz coil is connected in the culture box body, and the culture box body is connected with a supporting assembly; the supporting assembly is slidably connected with a storage plate. The storage plate is located between two coils of the Helmholtz coil, and the storage plate is used for placing a culture dish; a low-frequency pulse electromagnetic field generator and an oscilloscope are integrated on the upper portion of the incubator body, and the Helmholtz coil is connected to a control panel on the upper portion of the incubator body through an internal wire. The problems that an existing cell culture box is easily infected by bacteria and fungi due to repeated cell transfer, the time for irradiating a cell culture dish by an electromagnetic field cannot be accurately controlled, and a large amount of manpower and material resources are consumed due to repeated parameter modification, cell culture dish movement and cable connection are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and particularly to a low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator. Background Art

[0002] A carbon dioxide cell incubator is a commonly used device in biological and medical research. It includes an incubator body and a control panel for setting parameters at the upper part, and is mainly used to maintain the environmental conditions required for cell growth. This device simulates the physiological environment in the human body or other organisms by controlling temperature, humidity, and carbon dioxide concentration, so as to support the growth and reproduction of cells. Generally, a carbon dioxide cell incubator is designed in a water jacket type or direct heating type to precisely adjust the temperature inside the incubator. A humidity control device is used to maintain high humidity to avoid drying of the culture medium, and an air filter is used to effectively remove microorganisms and particulate matter in the air to ensure the cleanliness of the culture environment. And it is necessary to keep the inside clean during use to prevent cross-contamination. It is widely used in cell and tissue culture and the culture of certain special microorganisms, and is commonly found in research fields such as cell kinetics research, collection of mammalian cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, culturing hybridoma cells to produce antibodies, in vitro fertilization (IVF), stem cells, tissue engineering, drug screening, etc.

[0003] In the experiment of stimulating cells with electromagnetic fields, since the cell culture dishes of the set groups need to be placed in an electromagnetic field generator with different magnetic field parameters for irradiation, and after a certain time, the cell culture dishes of other groups need to be replaced. During this period, it is necessary to adjust the electromagnetic field parameters multiple times, repeatedly open and close the incubator door, transfer the cell culture dishes to different devices multiple times, irradiate for a certain time and then irradiate again after an interval of time, etc. There are many details in the traditional method that affect the experimental accuracy or even lead to experimental failure during this period: 1 Repeatedly opening and closing the incubator door and transferring the cell culture dishes will cause the cells to be easily contaminated by bacteria / fungi in the air; 2 Transferring the cell culture dishes multiple times a day will cause the cells in the culture dishes to repeatedly enter the dormant and resuscitation stages, making it difficult for the cells to grow continuously and affecting the experimental accuracy; 3 Manually timing the irradiation time will cause errors, with an error of several seconds or minutes each time, and serious errors will accumulate in the experiment; 4 Conducting multiple experiments a day consumes a lot of manpower and material resources, and manually timing the interval time each time will cause errors. 5 Different electromagnetic field parameters will affect the surrounding environmental temperature, and different temperatures will affect cell growth, generating interference factors and being unfavorable for the experiment. Summary of the Invention

[0004] The present invention aims to provide a low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator, which solves the problems that the existing cell incubator is vulnerable to bacterial and fungal infections due to repeated cell transfer, the irradiation time of the electromagnetic field on the cell culture dish cannot be precisely controlled, and a large amount of manpower and material resources are consumed by repeatedly modifying parameters and moving the connection cables of the cell culture dish.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator, comprising an incubator body, a Helmholtz coil is connected inside the incubator body, and the incubator body is connected with a support assembly; the support assembly is slidably connected with a placement plate; and the placement plate is located between two coils of the Helmholtz coil, and the placement plate is used for placing culture dishes; a low-frequency pulsed electromagnetic field generator and an oscilloscope are integrated on the upper part of the incubator body, and the Helmholtz coil is connected to the control panel on the upper part of the incubator body through internal wires.

[0007] Further, the support assembly includes two sets of oppositely arranged support frames, each support frame includes a plurality of support rods, one end of the support rod is connected to the bottom of the incubator body, and the other end of the support rod is connected with a chute, and sliding rails are respectively connected to both side edges of the bottom of the placement plate, and the sliding rails are slidably connected in the chute.

[0008] Further, the two sets of support frames are symmetrically arranged with respect to the vertical plane where the axis of the Helmholtz coil is located, and a limiting plate is connected to one end of the chute away from the door of the incubator body.

[0009] Further, the cross-sectional shapes of the chute and the sliding rail are both "convex" shaped.

[0010] Further, a locking assembly for restricting the sliding of the placement plate is provided at one end of any chute close to the door of the incubator body.

[0011] Further, a plurality of through holes are evenly spaced on the placement plate.

[0012] Further, the electromagnetic field parameters generated by the low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator are: the waveform width is 5 ms, the pulse width is 0.2 ms, the pulse interval is 0.02 ms, and the pulse group interval is 60 ms.

[0013] The principle and beneficial effects of the technical solution are:

[0014] 1. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator provided by the present invention. The incubator body is used to provide the temperature, carbon dioxide concentration and humidity suitable for cell growth and differentiation, and eliminates the heat generated by different magnetic field intensities of the electromagnetic field through the cell life maintenance and constant temperature system of the carbon dioxide constant temperature incubator body, so as to eliminate variable factors within a certain period of time and focus on the scientific research on cell growth and differentiation under the action of the electromagnetic field; A Helmholtz coil is connected inside the incubator body, and a low-frequency pulsed electromagnetic field generator and an oscilloscope are integrated on the upper part of the incubator body. The Helmholtz coil is connected to the control panel on the upper part of the incubator body through internal wires. The Helmholtz coil is used to generate a uniform magnetic field and is composed of a pair of completely identical circular conductive coils. These two coils are placed parallel and coaxial, and the distance between them is equal to half of their respective radii. When the same-direction current passes through the two coils, a relatively uniform magnetic field region can be formed near the midpoint of the center connection line of the two coils; The low-frequency pulsed electromagnetic field generator is integrated in the control panel on the upper part of the incubator. The low-frequency pulsed electromagnetic field generator is used to generate an electromagnetic field with a specific frequency, intensity, rise time and pulse width. As a physical adjuvant therapy to promote bone tissue formation and increase bone density, its mechanism principle and working parameters still have a large amount of research and exploration in the field of cell biology. Setting different parameters has different effects on different cells in promoting or inhibiting osteogenesis and cell proliferation; The oscilloscope is integrated in the control panel on the upper part of the incubator and is used to convert the electrical signal into a visual waveform image, enabling users to intuitively observe the change process of the signal, and can also accurately measure various parameters of the signal, such as voltage amplitude, frequency, period, phase difference, etc., and has a storage and playback function, enabling users to save the waveform data for subsequent analysis or reproduce the captured waveform. The incubator body is connected with a support component, and the support component is slidably connected with a placement plate, so that the placement plate can slide out or into the incubator body, and the placement plate is located between the two coils of the Helmholtz coil. The placement plate is used to place culture dishes.

[0015] By adjusting the parameters of the control panel, set the culture environment of the incubator body, the preset electromagnetic field parameters generated by the low-frequency pulsed electromagnetic field generator, and the radiation time of the electromagnetic field. Place the cell culture dish for the required experiment on the placement board inside the Helmholtz coil of the incubator. The control panel of the incubator will turn on the pulsed electromagnetic field at the set time, irradiate the cells according to the preset electromagnetic field parameters and preset time, turn off the pulsed electromagnetic field at the end time, and wait for the next turn-on. During this period, the carbon dioxide cell incubator continues to work and maintains the temperature stability inside the box, constantly monitors the temperature on the placement board inside the Helmholtz coil, and automatically adjusts to offset the heat generated when the pulsed electromagnetic field works. By treating the culture medium with the pulsed electromagnetic field, the activity of the culture medium can be effectively improved, promoting the growth and differentiation of cells. The pulsed electromagnetic field has a bactericidal effect, which can effectively inhibit bacteria and fungi in the culture medium and improve the hygiene quality of the culture medium. Moreover, the pulsed electromagnetic field has penetrability and can uniformly treat the culture medium, improving the uniformity of the culture medium. In addition, this device avoids repeated cell transfer, thus avoiding cell infection by bacteria and fungi, and does not cause the cells in the culture dish to repeatedly enter the dormant and resuscitation stages, enabling the cells to grow continuously and increasing the experimental accuracy. The irradiation time of the electromagnetic field on the cell culture dish can be precisely controlled, reducing the error of manual timing and saving manpower and material resources. The carbon dioxide cell incubator continues to work and maintains the temperature stability inside the box, constantly monitors the temperature on the placement board inside the Helmholtz coil, and automatically adjusts to offset the heat generated when the pulsed electromagnetic field works, avoiding the influence of different electromagnetic field parameters on the surrounding environmental temperature, reducing interference factors, and being beneficial to the experiment.

[0016] 2. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator provided by the present invention. The support assembly includes a plurality of support rods and chutes, which can avoid the support assembly from excessively occupying the space inside the incubator body and improve the circulation of carbon dioxide and moisture inside the incubator body, promoting the growth and differentiation of cells. And the placement board is evenly spaced with a plurality of through holes, further ensuring that the culture dish is in a suitable carbon dioxide and moisture environment. The two side edges at the bottom of the placement board are connected with slide rails, and the slide rails are slidably connected to the chutes. The cross-sectional shapes of the chutes and the slide rails are both "convex" shapes. The connection of the "convex" shaped slide rails and chutes can ensure the smoothness of the sliding and also play a guiding role, making the placement board always located in the middle of the coil. One end of any chute close to the door of the incubator body is provided with a locking component, which can prevent the placement board from sliding out of the chute during the process of culturing cells.

[0017] 3. The low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator provided by the present invention is such that one end of the chute away from the door of the incubator body is connected with a limiting plate, which can prevent the placement plate from sliding out of the two coils. The two sets of support frames are symmetrically arranged with respect to the vertical plane where the axis of the Helmholtz coils is located, which can ensure that the placement plate is in the middle position between the coils, increasing the magnetic field radiation effect and enabling the culture dishes on the placement plate to be in a uniform magnetic field, further promoting the growth and differentiation of cells. The wire passes through the inner wall of the incubator body and is connected to the control panel, and the wire is hermetically connected to the incubator body, which is convenient to operate and has a good effect. If the wire passes through the sealed door gap of the incubator body, it may cause abnormalities in the air pressure, temperature, humidity, and carbon dioxide concentration inside the incubator body. During actual cultivation, the incubator body may emit a beeping alarm sound, affecting the experimental accuracy. The electromagnetic field parameters generated by the low-frequency pulsed electromagnetic field generator are: waveform width is 5 ms, pulse width is 0.2 ms, pulse interval is 0.02 ms, and pulse group interval is 60 ms. By designing reasonable low-frequency pulsed electromagnetic field parameters, cell proliferation and stem cell differentiation can be better promoted. Description of the Drawings

[0018] Figure 1 is a schematic structural diagram of a low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator of the present invention;

[0019] Figure 2 is Figure 1 an enlarged structural diagram at A in

[0020] Figure 3 is a top view of a low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator of the present invention;

[0021] Figure 4 is Figure 3 a partial cross-sectional view taken along B-B in

[0022] Figure 5 is a magnetic field waveform diagram generated by the low-frequency pulsed electromagnetic field generator of the present invention.

[0023] The names of the corresponding marks in the drawings are:

[0024] 1. Incubator body; 2. Helmholtz coils; 3. Support rod; 4. Chute; 5. Placement plate; 6. Through hole; 7. Culture dish; 8. Slide rail; 9. Limiting plate; 10. Hook; 11. Stop bar; 12. Low-frequency pulsed electromagnetic field generator; 13. Oscilloscope. Detailed Embodiment

[0025] The present invention will be further described in detail below in conjunction with the drawings and embodiments:

[0026] As Figures 1 to 5As shown in the figure, a low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator includes an incubator body 1. Inside the incubator body 1, a Helmholtz coil 2 is connected, and the incubator body 1 is connected with a support component. The support component is slidably connected with a placement plate 5, so that the placement plate 5 can slide out or into the incubator body 1; a number of through holes 6 are evenly spaced on the placement plate 5; specifically, the support component includes two sets of oppositely arranged support frames, and the two support frames are symmetrically arranged with respect to the vertical plane where the axis of the Helmholtz coil 2 is located; the support frame includes two support rods 3. One end of the two support rods 3 is connected to the bottom of the incubator body 1, and the other end of the support rod 3 is connected with a chute 4 with a "convex" cross-sectional shape. On both sides of the bottom of the placement plate 5, edges are connected with sliding rails 8 with a "convex" cross-sectional shape. The sliding rails 8 are slidably connected in the chute 4. At one end of the right chute 4 close to the door of the incubator body 1, a locking component is arranged. The locking component includes a hook 10 rotatably connected to the end of the chute 4. A stop rod 11 is connected to the position of the chute 4 corresponding to the hook 10; a limiting plate 9 is connected to the end of the chute 4 away from the door of the incubator body 1; the placement plate 5 is located between the two coils of the Helmholtz coil 2, and the placement plate 5 is used for placing culture dishes 7; on the upper part of the incubator body 1, a low-frequency pulsed electromagnetic field generator 12 and an oscilloscope 13 are integrated. The Helmholtz coil 2 is connected to the control panel on the upper part of the incubator body 1 through internal wires, and the internal wires are hermetically connected to the inner wall of the incubator body 1. The electromagnetic field parameters generated by this low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator are: waveform width is 5 ms, pulse width is 0.2 ms, pulse interval is 0.02 ms, and pulse train interval is 60 ms.

[0027] The specific implementation process is as follows:

[0028] When using this incubator, disinfect each component, adjust the culture environment parameters (temperature, humidity, carbon dioxide concentration) of the incubator body 1 and the electromagnetic field parameters generated by the low-frequency pulsed electromagnetic field generator 12, and set the start and end times of electromagnetic field radiation. Pull out the placement plate 5, evenly and spacedly place the culture dishes 7 on the placement plate 5, avoiding the position where the sliding rail 8 is located during placement. Push the placement plate 5 into the incubator body 1, rotate the hook 10 to make it buckle on the stop rod 11, close the door of the incubator body 1, start the incubator body 1, and carry out cell culture.

[0029] Among them, the present invention provides a low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator. The incubator body 1 is used to provide a temperature, carbon dioxide concentration, and humidity suitable for cell growth and differentiation, and eliminates the heat generated by different magnetic field intensities of the electromagnetic field through the cell life support and constant temperature system of the carbon dioxide constant temperature incubator body 1, so as to eliminate variable factors within a certain period of time and focus on the scientific research of cell growth and differentiation under the action of the electromagnetic field; a Helmholtz coil 2 is connected inside the incubator body 1, and a low-frequency pulsed electromagnetic field generator 12 and an oscilloscope 13 are integrated on the top control panel of the incubator body 1. The Helmholtz coil 2 is connected to the control panel of the incubator body 1; the incubator body 1 is connected with a support assembly, and the support assembly is slidably connected with a placement plate 5, so that the placement plate 5 can slide out or into the incubator body 1, and the placement plate 5 is located between the two coils of the Helmholtz coil 2. The placement plate 5 is used to place the culture dish 7. By adjusting the parameters of the control panel, the culture environment of the incubator body 1, the preset electromagnetic field parameters generated by the low-frequency pulsed electromagnetic field generator 12, and the radiation time of the electromagnetic field are set. The cell culture dish 7 for the required experiment is placed on the placement plate 5 inside the Helmholtz coil 2 of the incubator. The control panel of the incubator will turn on the pulsed electromagnetic field at the set time, irradiate the cells according to the preset electromagnetic field parameters and preset time, turn off the pulsed electromagnetic field at the end time, and wait for the next turn-on. During this period, the carbon dioxide cell incubator continuously works and maintains the temperature of the internal environment stable, constantly monitors the temperature on the placement plate 5 inside the Helmholtz coil 2, and automatically adjusts to offset the heat generated during the operation of the pulsed electromagnetic field. By treating the culture medium with the pulsed electromagnetic field, the activity of the culture medium can be effectively improved, promoting cell growth and differentiation. The pulsed electromagnetic field has a bactericidal effect, which can effectively inhibit bacteria and fungi in the culture medium and improve the hygienic quality of the culture medium; moreover, the pulsed electromagnetic field has penetrability and can uniformly treat the culture medium, improving the uniformity of the culture medium; in addition, this device also avoids repeatedly transferring cells, thereby avoiding cell infection by bacteria and fungi, and will not cause the cells in the culture dish 7 to repeatedly enter the dormant and resuscitation stages, enabling the cells to grow continuously and increasing the experimental accuracy; the irradiation time of the electromagnetic field on the cell culture dish 7 can be precisely controlled, reducing the error of manual timing and saving manpower and material resources; the carbon dioxide cell incubator continuously works and maintains the temperature of the internal environment stable, constantly monitors the temperature on the placement plate 5 inside the Helmholtz coil 2, and automatically adjusts to offset the heat generated during the operation of the pulsed electromagnetic field, avoiding the influence of different electromagnetic field parameters on the surrounding environment temperature, reducing interference factors, and being beneficial to the experiment.

[0030] The support component package adopts several support rods 3 and chutes 4, which can avoid the excessive occupation of the space inside the incubator body 1 by the support components, and improve the circulation of carbon dioxide and moisture inside the incubator body 1, promoting the growth and differentiation of cells; and the placing plate 5 is evenly spaced with several through holes 6, further ensuring that the culture dish 7 is in a suitable carbon dioxide and moisture environment. The connection between the "convex"-shaped slide rail 8 and the chute 4 can ensure the smoothness of the sliding and also play a guiding role, making the placing plate 5 always located in the middle of the coil; the locking component can prevent the placing plate 5 from sliding out of the chute 4 during the process of culturing cells.

[0031] The limiting plate 9 can prevent the placing plate 5 from sliding out of the two coils. The two groups of support frames are symmetrically arranged with respect to the vertical plane where the axis of the Helmholtz coil 2 is located, which can ensure that the placing plate 5 is located in the middle position between the coils, increasing the magnetic field radiation effect, and making the culture dishes 7 on the placing plate 5 all in a uniform magnetic field, further promoting the growth and differentiation of cells. The electromagnetic field parameters generated by the low-frequency pulsed electromagnetic field carbon dioxide cell incubator are: the waveform width is 5 ms, the pulse width is 0.2 ms, the pulse interval is 0.02 ms, and the pulse group interval is 60 ms. By designing reasonable low-frequency pulsed electromagnetic field parameters, it can better promote cell proliferation and stem cell differentiation.

[0032] The above are only the embodiments of the present invention. Specific technical solutions or common knowledge such as characteristics known in the art are not described in detail here. It should be noted that for those skilled in the art, without departing from the technical solution of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the implementation effect of the present invention and the practicality of the patent. The protection scope required by this application should be based on the content of its claims, and the specific implementation manners described in the specification can be used to explain the content of the claims.

Claims

1. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator, characterized in that, It includes an incubator body, a Helmholtz coil is connected inside the incubator body, and the incubator body is connected with a support assembly; the support assembly is slidably connected with a storage plate; and the storage plate is located between the two coils of the Helmholtz coil, and the storage plate is used for placing culture dishes; a low-frequency pulsed electromagnetic field generator and an oscilloscope are integrated on the upper part of the incubator body, and the Helmholtz coil is connected to the control panel on the upper part of the incubator body through internal wires.

2. The low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator according to claim 1, wherein, The support assembly includes two sets of oppositely arranged support frames, each support frame includes a plurality of support rods, one end of the support rod is connected to the bottom of the incubator body, and the other end of the support rod is connected with a chute, and sliding rails are respectively connected to the two side edges of the bottom of the storage plate, and the sliding rails are slidably connected in the chutes.

3. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator according to claim 2, characterized in that, The two sets of support frames are symmetrically arranged with respect to the vertical plane where the axis of the Helmholtz coil is located, and a limiting plate is connected to one end of the chute away from the door of the incubator body.

4. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator according to claim 2, characterized in that, The cross-sectional shapes of the chute and the sliding rail are both "convex" shaped.

5. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator according to claim 2, characterized in that, A locking assembly for restricting the sliding of the storage plate is provided at one end of any one of the chutes close to the door of the incubator body.

6. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator according to claim 1, characterized in that, The storage plate is evenly spaced with a plurality of through holes.

7. A low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator according to claim 1, characterized in that, The electromagnetic field parameters generated by the low-frequency pulsed electromagnetic field carbon dioxide constant temperature cell incubator are: the waveform width is 5 ms, the pulse width is 0.2 ms, the pulse interval is 0.02 ms, and the pulse group interval is 60 ms.

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