101 results about "Cell damage" patented technology

A YL1-tiRNA for promoting mouse muscle cell injury repair belongs to the technical field of molecular biology, the YL1-tiRNA is formed by cutting mature mt-Tv, a phosphate group is added at the 5th terminal, phosphorylation modification is carried out on the sequence, and a section of sequence is added at the 5th terminal without changing the function of the sequence, the YL1-tiRNA is characterized in that the nucleotide sequence of the YL1-tiRNA is shown as Seq ID No: 1, and the nucleotide sequence of the YL1-tiRNA is shown as Seq ID No: 1. Experiments prove that the YL1-tiRNA can promote mouse muscle injury repair by regulating proliferation of C2C12 cells.

This invention provides specific sequence nucleic acid molecules and their applications as PDRNs, relating to the field of biotechnology. This invention yields novel specific sequence nucleic acid molecules with deterministic sequences, stable biological activity, and stable product quality. The PDRNs with these specific sequences are non-cytotoxic, exhibit high safety, and have broad application prospects. Furthermore, this invention is the first to discover that specific sequence PDRNs, compared to extracted PDRNs, significantly promote VEGF expression and effectively repair cell damage, providing a novel skincare and repair ingredient for the cosmetics and pharmaceutical industries. This invention also provides a simple method for preparing specific sequence PDRNs, suitable for widespread application and industrial production.

The invention relates to the technical field of aluminum electrolysis, and provides an aluminum electrolysis cell damage inversion method based on a machine learning algorithm, and the method comprises the following steps: obtaining the current data of an aluminum electrolysis cell through an optical fiber current sensor, and obtaining the temperature data of the aluminum electrolysis cell; carrying out blanking area division according to a blanking device of the aluminum electrolysis cell; performing regional division on the current data and the temperature data, and performing feature extraction on the current data and the temperature data; establishing a physical information forward model based on a neural network, and performing association mapping on the damage state vector, the current characteristic data and the temperature characteristic data through the physical information forward model; constructing a cathode joint loss function and an anode joint loss function; and respectively carrying out iterative solution on the cathode joint loss function and the anode joint loss function through an optimization algorithm, outputting an optimal damage state vector, and generating a damage space distribution result of the electrolytic cell. According to the invention, online inversion and spatial distribution visualization of the damage states of the anode and the cathode can be realized.

The invention discloses gamma-aminobutyric acid and application of a pharmaceutical composition thereof in preparation of drugs for preventing or treating aortic dissection and aortic aneurysm. The invention provides the application of gamma-aminobutyric acid in preparation of drugs for preventing or treating aortic dissection for the first time, and verifies the application of gamma-aminobutyric acid in preparation of drugs for preventing and / or treating aortic dissection and aortic aneurysm by inhibiting endothelial cell dysfunction; the application of gamma-aminobutyric acid in preparation of drugs for preventing and / or treating aortic dissection and aortic aneurysm by inhibiting expression of c-FOS to reduce vascular endothelial cell injury is verified; the application of gamma-aminobutyric acid in preparation of drugs for preventing and / or treating aortic dissection and aortic aneurysm by reducing infiltration of macrophages is verified.

The application discloses application of a miR-8069 inhibitor and belongs to the technical field of biological medicines.The inventor of the application finds that the expression of miR-8069 in the plasma of IS patients is abnormally increased in the research on the pathogenesis of IS, and through further cell experiments, it is found that inhibiting the expression of miR-8069 can effectively improve the adverse effects of OGD / R treatment on cell viability, apoptosis and ROS, and has a significant therapeutic effect on IS. Cell damage induced by OGD / R is often used as a model of cerebral stroke in vitro in research. The miR-8069 inhibitor disclosed by the application is used for preparing a medicine for treating cerebral stroke, and the nucleotide sequence is as follows: ACCGCCGACCGCCCCCAACCAUCC.

The application relates to the field of nanobiomedical technology, and particularly relates to application of polyethylene glycol modified black phosphorus nanosheets in prevention and treatment of acute liver injury. The polyethylene glycol modified black phosphorus nanosheets not only have a significant active oxygen scavenging capacity, but also have calcium ion chelation and buffering capacity, can simultaneously regulate intracellular oxidation-reduction homeostasis and calcium homeostasis, inhibit intracellular calcium overload, especially mitochondrial calcium overload, maintain mitochondrial function, and further reduce liver cell damage, apoptosis and inflammatory response, and play a liver protection role. The application finds that the black phosphorus nanosheets are used in calcium ion chelation and inhibition of cell calcium overload, and breaks through the limitation that the black phosphorus nanosheets are mainly used as active oxygen scavenging materials in the prior art. The material can also adsorb copper ions and realize radioactivity copper labeling without chelating agent, and can be used for liver enrichment tracing and integrated diagnosis and treatment application. The material has a significant protective effect on paracetamol-induced acute liver injury.

The invention discloses a composite medicine coating for treating pulmonary arterial hypertension, and belongs to the technical field of medical instruments. The coating is composed of an inert protective layer and a composite drug coating, the inert protective layer is a polyamino acid polymer, responds to pH change and is rapidly disintegrated only in the pH environment of a target site, and the loss of the composite drug coating in the delivery process is reduced; the composite drug coating is a composite component containing two or more gene regulatory factors and / or drugs, achieves a synergistic effect through a signal path, cells and a gene level, more efficiently regulates pulmonary artery endothelial cell dysfunction and smooth muscle cell injury, and has precise targeting and irreplaceability.

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

Systems and methods of the present disclosure provide a service loop for a pouch cell tab by bending the service loop into the cord length at the cell-level. Embodiments of the present disclosure reduces the risk of cell damage or open circuit welds. Both failure modes have the potential to open circuit a battery mid-operation or trigger a thermal runaway. According to various embodiments, the service loop is integral to the pouch such that no additional parts are used to form the pouch cell.

The application discloses an application of a miR-4680-3p inhibitor and belongs to the technical field of biological medicines.The inventor of the application finds that the expression of miR-4680-3p in the plasma of IS patients is abnormally increased in the research on the pathogenesis of IS, and through further cell experiments, it is found that inhibiting the expression of miR-4680-3p can effectively improve the adverse effects of OGD / R treatment on cell viability, apoptosis and ROS, and has a significant therapeutic effect on IS. Cell damage induced by OGD / R is often used as a classic model of cerebral apoplexy in vitro in research. The miR-4680-3p inhibitor disclosed by the application is used for preparing a medicine for treating ischemic apoplexy, and the nucleotide sequence of the miR-4680-3p inhibitor is as follows: UAACAACUCUUACAAUUCAGA.

The invention relates to the technical field of biomedical detection, and discloses an umbilical cord blood unfreezing reference specimen cell counting and flow cytometry dilution method which comprises the following steps: S1, preparing an unfreezing solution which is composed of HES, AB plasma and PBS, and placing the unfreezing solution at room temperature for later use; s2, taking the unfrozen umbilical cord blood unfreezing reference specimen, adding an unfreezing solution into the umbilical cord blood, firstly gently and uniformly mixing, then standing at room temperature, then continuing to add the unfreezing solution, gently and uniformly mixing again, then standing at room temperature, and stabilizing; s3, taking the diluted umbilical cord blood, and carrying out cell counting by adopting a counting instrument; and S4, taking the diluted umbilical cord blood again, and dyeing. The unfreezing solution composed of HES, AB plasma and PBS synergistically regulates osmotic pressure, provides cell nutrition, stabilizes the pH value, reduces the damage of the cryoprotectant to cord blood cells, maintains the cell activity, and improves the problem of result deviation caused by direct detection of cell damage in the prior art.

The present application relates to the technical field of aluminum electrolysis, and provides an aluminum electrolysis cell damage inversion method based on a machine learning algorithm, comprising the following steps: obtaining current data of an aluminum electrolysis cell through a fiber-optic current sensor, and obtaining temperature data of the aluminum electrolysis cell; performing discharge area division according to a discharger of the aluminum electrolysis cell; performing area division on the current data and the temperature data, and performing feature extraction on the current data and the temperature data; establishing a physical information forward model based on a neural network, and associating and mapping a damage state vector with current feature data and temperature feature data through the physical information forward model; constructing a cathode joint loss function and an anode joint loss function; respectively performing iterative solution on the cathode joint loss function and the anode joint loss function through an optimization algorithm, outputting an optimal damage state vector, and generating a damage space distribution result of the electrolysis cell. The present application can realize online inversion and spatial distribution visualization of anode and cathode damage states.

The invention discloses an electrical impedance-based high-pressure injector intravenous injection leakage detection alarm device, which comprises a measuring electrode band, a high-pressure injector and a detection alarm device, and is characterized in that the measuring electrode band comprises a flexible ring band, a measuring electrode and a data line, and the detection alarm device is integrated with a power supply module, a data processing module and an alarm module. The device has the beneficial effects that the device is small in cell damage, low in price, high in usability, safe, reliable and small in size, and can be integrated in a high-pressure injector to be used in linkage with the high-pressure injector; high-pressure injection liquid leakage electrical impedance data and normal data are learned in advance through a trained improved support vector regression model prediction algorithm model, and an alarm threshold point is predicted, so that the accuracy of an alarm threshold can be effectively improved, injection liquid leakage of a high-pressure injector can be well detected, an alarm can be automatically given, and injection operation can be stopped. Various complications and sequelae caused by liquid leakage can be well prevented, and double guarantees are provided for patients and hospitals.

Systems and methods of the present disclosure provide a service loop for a pouch cell tab by bending the service loop into the cord length at the cell-level. Embodiments of the present disclosure reduces the risk of cell damage or open circuit welds. Both failure modes have the potential to open circuit a battery mid-operation or trigger a thermal runaway. According to various embodiments, the service loop is integral to the pouch such that no additional parts are used to form the pouch cell.

The present invention relates to a method for attenuating retinal cell damage and other neuronal cell damage outside the central nervous system associated with reduced supply of neurotrophin, i.e., nerve growth factor (NGF), induced by glaucoma and other forms of neurodegeneration by orally administering a therapeutically effective amount of an aqueous extract of spearmint to a subject.

The present application relates to the technical field of probiotic composition, and discloses a bacterial agent for regulating vaginal microbial flora and a preparation method thereof, which comprises the following steps: preparing Lactobacillus paracasei KFY202407 powder into a seed solution; performing segmented fermentation culture on the seed solution; obtaining bacterial slurry after centrifugal enrichment of the fermentation product; mixing the bacterial slurry with a composite freeze-drying protective agent and then performing freeze-drying to obtain freeze-dried bacterial powder; and performing gelatinization treatment on the freeze-dried bacterial powder to obtain the bacterial agent. The present application realizes rapid proliferation of bacterial cells and promotes accumulation of active metabolites by adopting a two-stage fermentation process of liquid-state fermentation followed by solid-state fermentation, thereby solving the technical problems of low active bacterial proliferation efficiency and insufficient metabolites in the prior art. Meanwhile, the present application adopts a freeze-drying process of two-stage pre-freezing and drying sublimation, so that ice crystals are removed in the form of sublimation instead of melting, thereby solving the technical problem of bacterial cell damage and uncontrollable inactivation due to ice crystals in the traditional freeze-drying technology.

The application belongs to the field of treating blood vessel-bone coupling imbalance diseases in traditional Chinese medicine, and particularly relates to a composition for treating blood vessel-bone coupling imbalance diseases, a pharmaceutical preparation and application. Geniposide alone or in combination with crocin I can effectively reduce endothelial cell damage under pathological stimulation conditions, reduce the level of cell apoptosis and the degree of oxidative stress, improve the vascular endothelial function state, and can also significantly inhibit osteoclast differentiation, reduce the number of mature osteoclasts, and reduce bone resorption activity, thereby being conducive to restoring the dynamic balance between bone formation and bone resorption.

The invention relates to the technical field of pulse output, and provides a high-voltage pulse output method, a high-voltage pulse output circuit and electric transduction equipment. The method comprises the steps that based on a preset output mode, a first high-voltage pulse output module and a second high-voltage pulse output module are controlled to output high-voltage pulses in a target mode; the preset output mode comprises a double-voltage pulse output mode and a single-voltage pulse output mode, the target mode comprises an alternate output mode and a simultaneous output mode, and the first high-voltage pulse output module and the second high-voltage pulse output module are connected in parallel. According to the application, the two high-voltage pulse output modules are connected in parallel, and the two high-voltage pulse output modules can output alternately or simultaneously, so that the adjustment range of the high-voltage pulse width can be effectively expanded, high-voltage perforation of cells and low-voltage introduction of allogenic materials are realized, and the application can be suitable for transduction of various types of cells; and the adjustment efficiency of the high-voltage pulse voltage is effectively improved, cell damage is avoided, and the transconductance is improved.

The invention belongs to the technical field of biological detection, and particularly relates to a multichannel circulating epithelial cell separation and enrichment device based on microfluidic immunocapture and application. The device comprises a fluid injection unit, a switching valve, a three-layer structure chip body, a reagent bearing module, a bar code scanning module, a temperature control module and an exhaust air cooling system, wherein a multi-channel micro-fluidic structure, an interface micro-column regulation and control region and a three-dimensional structure enrichment cavity are integrated in the chip body. A switching valve is controlled to automatically switch a sample injection channel through a dynamic scoring algorithm, and precise capture of target cells in a multi-channel enrichment cavity is realized by combining microstructure regulation and control and a temperature difference induction mechanism. The device has the advantages of high capture efficiency, low cell damage, large flux and high intelligent degree, and is suitable for enrichment detection of rare cells such as circulating tumor cells.

The embodiment of the invention provides an electrochemical device and electric equipment. The electrochemical device comprises an electrode assembly, the electrode assembly comprises a first pole piece, a diaphragm and a second pole piece which are sequentially stacked in the first direction, the pole piece on the outermost layer of the electrode assembly in the first direction is a first single-face pole piece, and the first single-face pole piece comprises a second current collector and a second active layer; the second current collector comprises a first foil layer, a polymer layer and a second foil layer which are stacked in sequence, in the orthographic projection of the plane where the first foil layer is located, the polymer layer and the second foil layer are both located in the first foil layer, the elastic modulus of the first foil layer is P1, the elastic modulus of the second foil layer is P2, P1 is smaller than P2, and P2 is larger than or equal to 70 Gpa and smaller than or equal to 500 Gpa. Therefore, the electrochemical device disclosed by the embodiment of the invention has the advantages of effectively avoiding interface debonding, cell damage or shell cracking and being good in safety and durability.

The invention discloses a fucoidin-marine mineral composition for promoting skin cell repair and application thereof, and belongs to the technical field of skin care products. The composition provided by the invention is derived from food or small-pollution deep sea, and is high in safety; various mineral substances and active ingredients are contained, so that the nutrition is rich; the components have a synergistic effect, can effectively reduce damage of light damage to cells and promote fibroblasts to secrete collagen, and have good effects on repairing cell damage, delaying skin aging and reducing damage caused by external and internal factors.

The utility model provides an immune cell long-term storage activity maintaining device, which relates to the technical field of cell storage and comprises a storage box, a storage cavity is arranged in the storage box, an arc cavity is arranged on the surface of the storage box, and four liquid nitrogen tank bodies are arranged in the arc cavity at equal intervals. According to the liquid nitrogen tank, the plurality of liquid nitrogen tank bodies are mounted in the arc cavity, so that the effect that a low-temperature environment in the storage cavity cannot be maintained due to the damage of one liquid nitrogen tank body can be prevented, and the effect of preventing the damage of cells due to the damage of the liquid nitrogen tank bodies can be achieved; when the liquid nitrogen in one liquid nitrogen tank body is exhausted, the other liquid nitrogen tank body can operate again, the effect that the low-temperature environment is not stable in the liquid nitrogen supplementing process due to the fact that the liquid nitrogen in the liquid nitrogen tank body is exhausted can be prevented, and therefore the effect of improving the stability of the low-temperature environment can be achieved.

The present application relates to the yunnanense alkaloid J extracted from the stem and leaf of yunnanense and its extraction method and application, which can effectively solve the drug problem of Alzheimer's disease, and the technical solution is that the yunnanense alkaloid J extracted from the stem and leaf of yunnanense has the following chemical molecular structure formula: the present application effectively extracts the alkaloid J (Pedatisectamine J) from the stem and leaf of yunnanense, and the compound can significantly improve Aβ 25‑35 Inducing PC-12 cell damage, effectively used for preparing AD treating drugs, enriching the new use of active ingredients in the stem and leaf of yunnanense, developing the medicinal value and commercial value of the stem and leaf of yunnanense, and having significant economic and social benefits.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to application of a related biological product of an StIQD20 gene to improvement of plant salt tolerance. The nucleotide sequence of the StIQD20 gene is shown as SEQ ID NO.1, the StIQD20 gene can regulate and control the influence of salt stress on the growth and development of potato seedlings, and a potato strain which is over-expressed with the StIQD20 gene and is cultivated by using related biological products of the StIQD20 gene shows remarkable agronomic advantages under the salt stress: under the treatment condition of 100 mmol / L NaCl, the salt stress of the potato strain is reduced, and the salt stress of the potato strain is reduced. The plant height, the leaf number and the fresh weight of a potato plant over-expressed with the StIQD20 gene are 1.2-3.8 times of those of a wild type, the activity of superoxide dismutase and peroxidase is improved by 1.25-3.3 times, and the malondialdehyde content is reduced by 10-70%. By using related biological products of the StIQD20 gene, the anti-oxidation defense capability of plants to salt stress can be improved, and cell damage is reduced.

The invention provides an improved method for separating liver parenchymal cells from non-parenchymal cells of a mouse with a non-alcoholic fatty liver disease induced by a high fat diet. According to the method, the NAFLD mouse model is successfully constructed by feeding a high-fat feed containing 60% of fat for 12-16 weeks. An improved two-step collagenase perfusion technology is adopted, a low-concentration enzyme solution is combined, and digestion time is accurately controlled, so that cell damage is effectively reduced. The efficient separation and purification of parenchymal hepatic cells and non-parenchymal cells are realized through a Percoll solution with a specific proportion by utilizing a density gradient centrifugation method. Flow cytometry and molecular biological technical verification show that the separated cells are high in survival rate and excellent in purity. The method provides a high-quality primary cell material for research on NAFLD pathogenesis and drug target discovery, and has important application value.

A low-cell-breakage-rate centrifugal tube for preparing platelet-rich plasma comprises a tube body, an upper tube cover is arranged on the tube body, an input channel is formed in the upper tube cover, a guide-in tube is arranged at the end, facing the tube body, of the input channel, and the guide-in tube is arranged obliquely towards the inner tube wall of the tube body; the upper pipe cover is further provided with a plurality of taking and conveying channels. The arrangement mode that the ingress pipe inclines towards the pipe body is adopted, drainage can be conducted through the inner wall of the pipe body, a very good buffering effect is achieved, damage to cells in the transferring process is avoided, due to the fact that the ingress pipe is short and does not make contact with blood and blood cells in the centrifugal process, damage to cells related to the catheter cannot be caused, and the cell transferring efficiency is improved. Therefore, the integrity of cells prepared from the obtained platelet-rich plasma is ensured, and the activity and clinical effect of the PRP are improved.

The present invention provides a luminescent nanoparticle. The light-emitting nanoparticles have light-emitting stability and light resistance, and are low in biotoxicity. The light-emitting nanoparticles comprise a parent material and a light-emitting substance contained in the parent material, the parent material containing at least one cationic element selected from the group consisting of Ti, Si, Ca, Al, and Zr, and at least one anionic element selected from the group consisting of O and P, the concentration of the light-emitting substances in the parent material is such that the average distance between the light-emitting substances is 1.2 nm or more.

This invention relates to the field of cell culture technology, specifically to a cell culture tank for producing porcine circovirus vaccine. The tank includes a main body with a feeding port at the top, a fixing box at the top, and a heat dissipation vent at the top of the fixing box. A stirring mechanism is located inside the main body. The first cone design achieves a shearing and flow-diverting effect, the arc surface guides the flow, reducing cell damage, and the spherical protrusions and through-holes further enhance the flow disturbance effect. The row of staggered spherical protrusions further improves the mixing effect. The two flow-dispersing blocks are arranged in opposite directions, achieving mixing in both forward and reverse rotation. The second cone design reduces resistance during reverse rotation without affecting mixing.

This invention relates to the field of microbial culture, and more specifically to a cell culture system and method. Different cell mixtures are stored separately in different culture dishes. Through drive-controlled adjustment, the different culture dishes are brought together. The brought-to-be-jointed cell mixtures fuse through compression. Peristaltic stirring effectively promotes mixing while avoiding cell damage. After resetting, the cell fusion is observed, and the system continues to combine and fuse different cell types through drive control. This promotes cell fusion, reaction, and storage, facilitating the observation of experimental data and preventing contamination. Multiple culture dishes are evenly fixed on a height-adjusting actuator. Height-adjusting actuators are installed in the central support platform and two side sliding platforms. The beneficial effect of this invention is that it ensures automatic fusion and observation of cells without contamination.

The present application relates to a kind of enrichment method of stem cell high purity positive cell group, belong to the field of biotechnology and cell engineering technology.It aims to solve the technical problems such as the cell damage of existing screening technology, low screening efficiency, easy to affect stem cell pluripotency and genome stability, etc.The present application forms 23-cell small mass after H9 human embryonic stem cells infected by lentivirus are digested gently, after gradient dilution static culture, high expression positive clone is screened under fluorescence microscope, and target cell group is directly enriched by visual mechanical scraping.The present application does not change cell genome, and is high in safety;Small mass inoculation significantly improves cell survival and cloning efficiency at low density;Selective scraping operation is accurate, fast and low in cost;The proportion of reporter gene (EYFP) positive cells in the obtained positive cell group can reach 93.2%, and good pluripotency and function are maintained, suitable for efficient enrichment of stem cells and immune cells.