58 results about "Peroxynitrite" patented technology

Reduction of the likelihood of adverse inflammatory reaction to an implant or a transplant is achieved through several mechanisms including the catalysis of isomerization of peroxynitrite by a hydrogel-bound peroxynitrite isomerization catalysts. A second mechanism controls acceptable and unacceptable dimensions of surface features of implants, such as vascular stents. A third mechanism fabricates implants from materials which are substantially free from alloys transition metals which produce ions of which catalyze cell killing radical formation.

This invention provides a novel class of substituted macrocyclic porphyrin compounds. The compounds are useful as peroxynitrite decomposition catalysts. Pharmaceutical compositions, and methods of making and using the compounds, or a pharmaceutically acceptable salt, hydrate, or prodrug thereof are also described.

Provided herein are compounds or fluorogenic probes which can be used as reagents for measuring, detecting and / or screening ROS or RNS such as peroxynitrite or hypochlorite. Provided also herein are methods that can be used to measure, directly or indirectly, the amount of peroxynitrite or hypochlorite in chemical samples and biological samples such as cells and tissues in living organisms. Specifically, the methods include the steps of contacting the fluorogenic probes disclosed herein with the samples to form one or more fluorescent compounds, and measuring fluorescence properties of the fluorescent compounds. Provided also herein are high-throughput screening fluorescent methods for detecting or screening peroxynitrite or compounds that can increase or decrease the level of peroxynitrite or hypochlorite in chemical and biological samples.

A compound represented by the following general formula (I):{R1, R3, R6 and R8 are methyl groups and the like, R2 and R7 are cyano groups and the like, R4 and R5 are fluorine atoms and the like, and X is a group represented by the formula (A) [R11 is a monocarboxy-substituted C1-8 alkoxy group and the like, R12, R14 and R15 are hydrogen atoms and the like, and R13 is hydroxy group and the like],provided that the combination of the aforementioned substituents is such a combination that the compound represented by the formula (I) can be substantially non-fluorescent before reacting with peroxynitrite, and the compound represented by the formula (I) in which the group represented by the formula (A) is nitrated after reacting with peroxynitrite can be substantially highly fluorescent}, or a salt thereof, which is useful as a fluorescent probe for peroxynitrite measurement.

This invention provides compositions which specifically reacts with peroxynitrite rather than other reactive oxygen species and reactive nitrogen species. This invention also provides related agents for measuring peroxynitrite. This invention also provides related methods for measuring peroxynitrite in a sample, high-throughput screening fluorescent methods for detecting peroxynitrite and high-throughput methods for screening compounds that increase or decrease the production of peroxynitrite comprising using such compositions and agents.

This invention provides a novel class of substituted macrocyclic metallic complexes. The complexes are useful as peroxynitrite decomposition catalysts. Pharmaceutical compositions, and methods of making and using the compounds, or a pharmaceutically acceptable salt, hydrate, prodrug, or mixture thereof are also described.

Provided herein are improved fluorogenic compounds and probes that can be used as reagents for measuring, detecting and / or screening peroxynitrite. The fluorogenic compounds of the invention can produce fluorescence colors, such as green, yellow, red, or far-red. Also provided herein are fluorogenic compounds for selectively staining peroxynitrite in the mitochondria of living cells. Provided also herein are methods that can be used to measure, directly or indirectly, the presence and / or amount of peroxynitrite in chemical samples and biological samples such as cells and tissues in living organisms. Also provided are high-throughput screening methods for detecting or screening peroxynitrite or compounds that can increase or decrease the level of peroxynitrite in chemical and biological samples.

The present invention is directed to a composition and method of preserving organs comprising exposing said organs to a preservation solution that includes at least an inhibitor or quencher of peroxynitrite. In its most easily formulated embodiment the present invention is a modified Wisconsin solution which includes the inhibitor or quencher as described herein.

The invention discloses a two-photon fluorescence probe for detecting peroxynitrite as well as a preparation method and application of the two-photon fluorescence probe. The two-photon fluorescence probe has the structural formula as shown in the description. The two-photon fluorescence probe is named as TPNIR-FP. The two-photon fluorescence probe adopts a Nile red derivative as a fluorescent group and an alpha-keto-amide functional group as an ONOO-reaction group, can rapidly respond to ONOO- (within 10 seconds), and in addition is high in sensitivity and good in selectivity. The two-photon fluorescence probe can be applied to imaging research on ONOO- in antharcycline antibiotic induced cardiac muscle cell and cardiac muscle tissue damage.

This invention provides a novel class of substituted macrocyclic porphyrin compounds. The compounds are useful as peroxynitrite decomposition catalysts. Pharmaceutical compositions, and methods of making and using the compounds, or a pharmaceutically acceptable salt, hydrate, or prodrug thereof are also described.

Exemplary methods and systems for killing or deactivating spores include applying a fluid to a surface containing a spore; and applying direct or indirect plasma to the surface for a period of time. In some embodiments, the fluid includes water. In some embodiments, the spore is Clostridium difficile and in some is Bacillus Anthracis. In some embodiments, the fluid is in the form of a vapor, a fog, a spray, a mist or an aerosol, which may be activated prior to or after applying the fluid to a surface. In some embodiments, peroxynitrite is created in the fluid during the method. Another exemplary embodiment of killing or deactivating a spore includes treating spores with direct plasma or indirect plasma for a period of time and applying an antimicrobial to the spores. In some embodiments, the antimicrobial is an alcohol, a bleach or an alcohol-based sanitizer.

This invention provides a novel class of substituted macrocyclic metallic complexes. The complexes are useful as peroxynitrite decomposition catalysts. Pharmaceutical compositions, and methods of making and using the compounds, or a pharmaceutically acceptable salt, hydrate, prodrug, or mixture thereof are also described.

The invention provides compositions and formulations comprising catechol and / or acetogenins and various combinations thereof; including various combinations with other ingredients including botanical preparations containing acetogenin, an extract of green tea or a therapeutically active flavanoid contained in green tea; and one or more quinones. The invention includes a pharmaceutical composition including a salt of rhodizonic acid, an OH anion-generating base, a non-toxic acid, a quinone, a salt-containing sulfite, catechol and optionally, an acetogenin and an extract of green tea. The compositions and formulations demonstrate positive effects against a broad variety of cancer, autoimmune diseases, viruses and provides antioxidant protection against peroxyl hydrophilic, peroxyl lipophilic, hydroxyl, peroxynitrite and super oxide radicals. The compositions and formulations can be used for nutritional and nutraceutical uses, and used as dietary supplements.

The invention discloses a preparation method of a fluorescent probe for distinguishing peroxynitrite and hypochlorite on the basis of xanthene and coumarin. According to the method, a xanthene and coumarin dimer is adopted as a fluorescent parent, diphenylphosphinyl chloride which specifically reacts with the peroxynitrite is introduced onto a coumarin parent, and a sulfur-containing five-memberedring which specifically reacts with the hypochlorite is introduced onto the xanthan; different kinds of fluorescence are emitted at different excitation wavelengths when the peroxynitrite and the hypochlorite are present alone or in combination. The fluorescent probe can realize high-selectivity and high-sensitivity detection of the peroxynitrite and hypochlorite and have a broad application prospect in the field of detection of active small molecules.

The invention belongs to the field of medical drugs, and particularly relates to a manganese dioxide coated drug-loaded mesoporous titanium dioxide nanoparticle, and a preparation method and application thereof. The preparation method of the nanoparticles comprises the following steps: firstly, preparing mesoporous titanium dioxide by utilizing a solvothermal method and controlling the reaction temperature and time, then wrapping a manganese dioxide shell layer by utilizing a redox reaction of potassium permanganate, and finally loading a drug through electrostatic adsorption. The nanoparticlehas the advantages of low cost, good stability, good biocompatibility and the like, active oxygen and NO can be generated under action of in-vitro ultrasound, and combined application of sonodynamictherapy and NO therapy is achieved. Peroxynitrite with higher cytotoxicity can be further generated by the active oxygen and NO, and the tumor killing effect is enhanced.

This invention provides a novel class of substituted macrocyclic metallic compounds. The compounds are useful as peroxynitrite decomposition catalysts. Pharmaceutical compositions, and methods of making and using the compounds, or a pharmaceutically acceptable salt, hydrate, or prodrug thereof are also described.

The invention discloses a peroxynitrite near infrared fluorescent probe ONP and a preparation method and an application thereof. The near infrared fluorescent probe ONP is obtained by combining boratewith a methylene blue framework. The structure is as shown in a formula I. The near infrared fluorescent probe ONP can trace an endogenous ONOO-signal in KA-induced epilepsy. The probe ONP can imageONOO- effectively and selectively in vitro and in vivo, can penetrate blood brain Barrier (BBB) effectively, and has a brain targeting characteristic. By means of the characteristics of the probe, up-regulation of ONOO- in a KA-induced epilepsy period is observed directly in vivo and in vitro. In addition, the invention discloses a method of screening an anti-epilepsy inhibitor by combining high connotation analysis and ONP, thereby providing simple and effective product and method for researching ONOO- in a biological system and screening an anti-epilepsy drug.

A reagent for measuring peroxynitrite comprising a compound represented by the following general formula (I): wherein R1 represents an amino group, or hydroxy group; R2 represents a 2-carboxyphenyl group; and X1 and X2 independently represent hydrogen atom, or a halogen atom (e.g., 2-[6-(4′-hydroxy)phenoxy -3H-xanthen-3-on-9-yl]benzoic acid, or 2-[6-(4′-amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid) or a salt thereof, which does not react with superoxide or nitrogen monoxide, a precursor of peroxynitrite, and specifically reacts with peroxynitrite.

The invention provides a fluorescent probe for detecting peroxynitrite. The probe has a general structure formula Ia or Ib. The probe is prepared by: (1) stirring R4-substituted o-aminothiophenol or R4-substituted o-aminophenol and R1 / R2-substituted benzaldehyde in a methanol solution at a room temperature for two hours to obtain Ia; and (2) performing oxidative condensation of R4-substituted o-aminothiophenol or o-aminophenol and R2-substituted o-hydroxybenzaldehyde to obtain a benzothiazole or benzoxazole intermediate, coupling triflate converted by the phenolic hydroxyl group of the intermediate and R3-substituted p-hydroxybenzene tert-butyldimethylsilylester / p-aminobenzene tert-butyldimethylsilylester, and performing methylation of aniline nitrogen atoms and hydroxy elimination of silylester protecting group to obtain Ib. The fluorescent probe provided in the invention can have fast specific reaction with peroxynitrite to generate a product with strong fluorescence, so that specific detection of peroxynitrite is realized. The general structure formula of the probe in the invention is shown in the description.

Methods for treating sexual dysfunction are disclosed. The methods generally comprise the administration of an NO donor and an antioxidant; the active ingredients can be administered topically to the genitals of the patient. The methods allow for localized NO delivery through topical application of the present compounds, while minimizing, if not preventing, damage associated with peroxynitrite formation. Compositions comprising L-arginine, or a derivative thereof and an antioxidant in a pharmaceutical carrier suitable for application to the genitals is also disclosed.

The present invention is a novel method using a MegaRibavirin aerosol or a MegaRibavirin combination of therapeutics for the treatment of viral disease particularly the pandemic influenza strains “swine” 2009 H1N1 and H5N1. This invention utilizes Ribavirin in an aerosol Mega Dose (61-161 mg / ml) alone or combined with or without other antivirals, a perfluorocarbon emulsion and anti-inflammatory / anti-oxidants. Where applicable, the perfluorocarbon emulsion may dissolve these agents enabling a depot effect and possible protracted delivery. In addition perfluorocarbon emulsions have the possible added benefit of oxygen carrying capacity and alveolar nitric oxide sequestration, which may reduce peroxynitrite formation and decrease Influenza severity.

The present invention relates to methods of preventing contrast-induced nephropathy including the step of administering an effective amount of a compound (e.g., a peroxynitrite decomposition agent, a PARP inhibitor or a superoxide dismutase mimic) to a subject to be administered a contrast agent.

The invention provides compositions and formulations comprising catechol and / or acetogenins and various combinations thereof; including various combinations with other ingredients including botanical preparations containing acetogenin, an extract of green tea or a therapeutically active flavanoid contained in green tea; and one or more quinones. The invention includes a pharmaceutical composition including a salt of rhodizonic acid, an OH anion-generating base, a non-toxic acid, a quinone, a salt-containing sulfite, catechol and optionally, an acetogenin and an extract of green tea. The compositions and formulations demonstrate positive effects against a broad variety of cancer, autoimmune diseases, viruses and provides antioxidant protection against peroxyl hydrophilic, peroxyl lipophilic, hydroxyl, peroxynitrite and super oxide radicals. The compositions and formulations can be used for nutritional and nutraceutical uses, and used as dietary supplements.

The invention relates to a high-selectivity and ultra-sensitive liver cancer cell specific peroxynitrite probe. Specifically, the probe is a naphthalimide compound and can be taken as a peroxynitritefluorescence probe to be used for detecting peroxynitrite in liver cancer cells. The probe can realize at least one of the following technical effects: peroxynitrite can be recognized with high selectivity; response to peroxynitrite can be rapidly realized; ultra-sensitive analysis for peroxynitrite can be realized; quantitative analysis for peroxynitrite can be realized; peroxynitrite in the liver cancer cells can be specifically detected; the property is stable, and the probe can be stored for a long time for use.

The invention relates to nanoparticles capable of controllably preparing ONOO<-> as well as a preparation method and application of the nanoparticles. Novel photosensitizer PEG-b-PAA-g-SNO@IR780 nanoparticles are designed and prepared based on the synergistic effect between nitric oxide and photodynamic therapy, and simple, efficient and controllable release of ONOO<-> is achieved based on single 808 nm laser stimulation. Under irradiation of single 808 nm laser, simple, efficient and controllable release of ONOO<-> is realized under single near-infrared light stimulation, and the nanoparticles are expected to provide a novel tool for simple, efficient and controllable release of peroxynitrite, and are used for exploring in-situ release of the peroxynitrite and physiological and pathological interaction thereof and used in the fields of antibacterial and anti-tumor treatment.

An anti-aging composition comprising a skin-beneficial amount of actives identified in Menyanthes trifoliata leaf, wherein the actives are inhibitors of one or more of MMP-1, 2 and 9 and / or scavengers of peroxynitrite. Also disclosed are methods of using such a composition, which include treating the skin for signs of chronological or pre-mature aging.

A compound represented by the following general formula (I):{R1, R3, R6 and R8 are methyl groups and the like, R2 and R7 are cyano groups and the like, R4 and R5 are fluorine atoms and the like, and X is a group represented by the formula (A) [R11 is a monocarboxy-substituted C1-8 alkoxy group and the like, R12, R14 and R15 are hydrogen atoms and the like, and R13 is hydroxy group and the like], provided that the combination of the aforementioned substituents is such a combination that the compound represented by the formula (I) can be substantially non-fluorescent before reacting with peroxynitrite, and the compound represented by the formula (I) in which the group represented by the formula (A) is nitrated after reacting with peroxynitrite can be substantially highly fluorescent}, or a salt thereof, which is useful as a fluorescent probe for peroxynitrite measurement.

Exemplary methods and systems for killing or deactivating spores include applying a fluid to a surface containing a spore; and applying direct or indirect plasma to the surface for a period of time. In some embodiments, the fluid includes water. In some embodiments, the spore is Clostridium difficile and in some is Bacillus Anthracis. In some embodiments, the fluid is in the form of a mist and in some is in the form of a vapor. In some embodiments, peroxynitrite is created in the fluid during the method. Another exemplary embodiment of killing or deactivating a spore includes treating spores with direct plasma or an indirect plasma for a period of time and applying an antimicrobial to the spores. In some embodiments, the antimicrobial is an alcohol, a bleach or an alcohol-based sanitizer.

The invention belongs to the field of fluorescent molecular probes, and discloses a coumarin fluorescent probe and a preparation method and application thereof. The preparation method comprises the following steps: synthesizing an intermediate 7-hydroxy-8-coumarin aldehyde by taking 7-hydroxycoumarin as a raw material, and synthesizing a second intermediate product 8-(benzo[d]thiazolyl-2-yl)-7-hydroxy-2H-benzopyran-2-one by taking the intermediate 7-hydroxy-8-coumarin aldehyde as a raw material, and performing substitution reaction on the second intermediate product 8-(benzo[d]thiazolyl-2-yl)-7-hydroxy-2H-benzopyran-2-one and 2, 4-dinitrobenzenesulfonyl chloride to synthesize the coumarin fluorescent probe. The fluorescent probe disclosed by the invention has specific selectivity on ONOO<-> and can be applied to in-vitro fluorescence detection of peroxynitrite.