13 results about "Peroxynitrite" patented technology

The application discloses a dual-response fluorescent probe and a preparation method and detection application thereof, and belongs to the technical field of fluorescent probes. The probe is prepared by condensation reaction of 4-methyl-7-hydroxy-8-aldehyde coumarin and 4-morpholine aniline, and can simultaneously and specifically respond to peroxynitrite and viscosity. ‑ The probe specifically reacts with ONOO to generate a concentration-dependent fluorescence enhancement, has high selectivity, fast response and good linearity, and the fluorescence is significantly enhanced with the increase of viscosity. The probe has lysosome targeting properties, and can realize real-time imaging and dynamic monitoring of endogenous and exogenous ONOO ‑ in living cells, and presents a high-contrast fluorescent signal in a non-alcoholic fatty liver model mouse liver tissue slice, so that ONOO ‑ overproduction and viscosity increase in a pathological state can be accurately identified.

This invention discloses a fluorescent probe for peroxynitrite, its preparation method, and its application, belonging to the field of analytical detection technology. The probe uses 4-bromo-1,8-naphthalenedicarboxylic anhydride and 4-(2-aminoethyl)morpholine as starting materials, and through a multi-step reaction, connects a 4-phenylboronic acid pinacol ester responsive group to obtain a benzisoquinoline-based fluorescent probe. The probe is a yellow solid, readily soluble in dimethyl sulfoxide, and exhibits no fluorescence before reacting with peroxynitrite. After the reaction, it emits strong yellow fluorescence at 575 nm under 500 nm excitation. The fluorescence intensity increases with increasing peroxynitrite concentration, with a detection limit as low as 0.13 μM. It exhibits high selectivity and good photostability. This probe can achieve real-time fluorescence imaging of endogenous peroxynitrite in hepatocytes and liver tissue of mice with acute liver injury. It can specifically identify peroxynitrite and is unaffected by thiols, metal ions, common enzymes, and other reactive oxygen species / reactive nitrogen species. It provides a highly sensitive and specific novel detection tool for the early diagnosis and molecular mechanism research of acute liver injury, and has significant scientific research and clinical application value.

The invention discloses a fluorescent probe for detecting peroxynitrite ions as well as a preparation method and application thereof, and relates to the technical field of biomedical detection, and the structural formula of the fluorescent probe is shown in the specification. The prepared fluorescent probe for detecting the peroxynitrite ions has high selectivity and high sensitivity on the peroxynitrite ions, the response speed is high, fluorescence stability can be achieved within about 30 minutes, ratio type fluorescent quantitative detection of the peroxynitrite ions in an aqueous solution is achieved, the linear correlation is good, and the detection sensitivity is high. The rapid detection of the peroxynitrite ion level in an oxidation modified low-density lipoprotein induced atherosclerosis cell model is also realized, and good biocompatibility and application potential are realized.

Provided is a method for spraying a mist containing peroxynitrous acid, which can be used in a wide range of applications such as finger disinfection applications and indoor spraying applications, and which is capable of changing the peak value of the concentration of peroxynitrous acid contained in the mist and the half-decay time of the concentration decay in accordance with the applications. In the spraying method, peroxynitrous acid is continuously generated by a spraying device from a plasma-activated mist having a pH in the range of 2 to 5, the plasma-activated mist being generated from an aqueous solution containing a nitrite salt having a concentration in the range of 10 micromoles to 10 mmoles.

This invention discloses a fluorescent probe for detecting peroxynitrite in living cells and its synthesis method. The fluorescent probe comprises an amphiphilic group, 4-amino-2-methoxyphenol as a recognition unit, and a rhodamine derivative fluorophore, wherein the recognition unit can interact with ONOO. ‑ A specific oxidation reaction occurs, causing significant changes in fluorescence intensity and emission wavelength, thereby enabling real-time imaging and quantitative analysis of peroxynitrite in the cellular microenvironment. The fluorescent probe of this invention possesses high selectivity, strong anti-interference capabilities, and rapid response, making it suitable for the detection of ONOO at the live-cell level. ‑ Dynamic observation.

This invention provides a 2-pyridinone-based afterglow chemiluminescent molecule, its preparation method, and its application. The Chinese name of the afterglow chemiluminescent molecule is (…). E )-2-(2-(6-((4-boronic acid benzyl)oxy)-7-fluoro-2,3-dihydro-1 H -oxanthracene-4-yl)vinyl)-3,3-dimethyl-1-(4-(3-methyl-2-oxopyridine-1-(2-yl)- ... H )-butyl)-3 H -Indole-1-onium. This invention also provides a method for preparing this afterglow chemiluminescent molecule. After being embedded in Pluronic F127 to form nanoparticles, the molecule exhibits excellent afterglow luminescence performance upon activation with oxynitrite in PBS buffer. This invention overcomes the challenges of irreversible substrate consumption, blue shift of emission, and low energy transfer efficiency in traditional organic afterglow systems by using 2-pyridone as a stable, non-consumable substrate and covalently linking it to the luminescent unit, providing a powerful new platform for precise molecular sensing and further disease diagnosis.

The invention discloses a rhodamine structure-based ONOOfluorescent probe as well as a preparation method and application thereof, a rhodamine skeleton is taken as a fluorescent chromophore, and free rotation of an aromatic amine unit is limited by constructing a rigid conjugate plane, so that the fluorescence quantum yield and the signal stability are improved; a quaternary ammonium salt type side chain is introduced to a rhodamine skeleton, and a cationic group is used for enhancing water solubility; an aryl borate recognition group is connected to a rhodamine parent nucleus through an aromatic bridging unit, and when ONOO is subjected to specific oxidation, a spontaneous deprotection process is carried out, so that ring opening of rhodamine and'lightening 'of fluorescence are triggered. The preparation method of the peroxynitrite fluorescent probe based on the rhodamine structure, disclosed by the invention, has the advantages of simple steps, mild conditions, easily available raw materials and higher yield, and is suitable for large-scale industrialization. The reagent is used as a high-sensitivity quantitative detection reagent for ONOO in a solution system; the fluorescent probe is used for fluorescence imaging monitoring of ONOO in living cells; the method is used for ONOO living body imaging and early evaluation in a drug-induced liver injury animal model.

The application discloses a hyaluronic acid modified co-loaded all-trans retinoic acid and arginine liposome and a preparation method and application thereof, and belongs to the field of pharmaceutical preparations.The liposome components comprise phospholipid, cholesterol, hyaluronic acid, all-trans retinoic acid and arginine.The prepared liposome can generate nitric oxide in response to a large amount of active oxygen existing in a liver fibrosis microenvironment, the generated nitric oxide is further oxidized into peroxynitrite by ROS, matrix metalloproteinase is activated, an extracellular matrix barrier is reduced, and nanoparticles are promoted to penetrate into a perisinusoidal space of the liver.The liver stellate cells are targeted through receptor-ligand specific combination, and the uptake efficiency of the liver stellate cells is improved.The released all-trans retinoic acid in cells is converted into a resting state by inducing activated liver stellate cells, and the regression of liver fibrosis diseases is promoted.The liposome penetrates through an extracellular matrix to promote nanoparticle penetration, enhances drug delivery, and provides a new way and strategy for efficient delivery of therapeutic drugs.

Selective probes provide tandem activity-based sensing and labeling of reactive oxygen species (ROS), such as hydrogen peroxide, peroxynitrite, and organic peroxides.

The invention relates to the technical field of fluorescent probes, and particularly discloses a peroxynitroso ion and viscosity difunctional fluorescent probe based on a cyanopyridine structure as well as a preparation method and application of the peroxynitroso ion and viscosity difunctional fluorescent probe based on the cyanopyridine structure, and the probe is formed by connecting cyanopyridine salt serving as a strong electron withdrawing group and naphthyl methyl ether serving as an electron donating group through an unsaturated C = C bond. When the viscosity changes, the probe realizes green fluorescence response to the viscosity by regulating and controlling a T-ICT mechanism. In the presence of ONOO <->, a C = C bond is subjected to oxidative cleavage, and a blue fluorescence signal is released, so that quantitative detection of ONOO <-> is realized. The probe has the advantages of large Stokes shift, high response speed, strong anti-interference capability, simple and efficient preparation method and the like, is successfully used for dual-channel fluorescence imaging of ONOO <-> and viscosity in cells, and provides an effective detection tool for researching a correlation mechanism of oxidative stress and microenvironment change.

The invention belongs to the technical field of fluorescent sensing detection, and particularly relates to an indole sulfonic acid-phenothiazine fluorescent probe as well as a preparation method and application thereof. The structural formula of the indole sulfonic acid-phenothiazine fluorescent probe is shown in the specification. The probe has a remarkable fluorescence enhancement change on peroxynitrite in a DMSO / water (7 / 3) solution (10mM, pH 7.4), is rapid in detection response (within 5 minutes), low in detection limit (29.19 nM) and excellent in detection selectivity, and is applied to fluorescence imaging detection of exogenous and endogenous peroxynitrite in living cells.

The application discloses a ratio fluorescent probe for detecting peroxynitrite and a preparation method and use method thereof. The application uses (E)-2-(3-(4-aminostyryl)-5,5-dimethylcyclohexyl-2-en-1-alkyl malonitrile to construct a classic intramolecular charge transfer system. When peroxynitrite exists, the peroxynitrite and an amide part on the probe molecule are oxidized to break and generate a strong electron donor-amino, and then the ICT effect of the molecule is enhanced, the emission wavelength is red-shifted, and the probe molecule emits strong red fluorescence. The ratio peroxynitrite probe of the dicyanisophorone dye provided by the application has good response performance to a peroxynitrite solution, can realize sensitive, rapid and quantitative detection of trace peroxynitrite in a sample, and has the advantages of simple operation, low cost, good water solubility and sensitive response.

The invention provides a nitrite peroxide responsive near-infrared ratiometric fluorescent probe as well as a preparation method and application thereof. The molecular structure of the fluorescent probe consists of a donor-pi-receptor type near-infrared fluorophore constructed by taking xanthene and cyano isophorone as mother nucleuses and a benzyl borate recognition group connected with a phenolic hydroxyl group of the fluorophore through a carbonate connecting bond. The probe is prepared through multi-step condensation and nucleophilic substitution reaction; the structure is clear, and synthesis conditions are mild. The fluorescent probe has concentration-dependent cascade activation response on nitrite peroxide: under low-concentration ONOO <->, a benzyl borate recognition group is hydrolyzed to generate a near-infrared emission intermediate CX-OH; under high-concentration ONOO <->, the intermediate is further oxidized to generate an emission blue shift product X-OH, so that ratio detection of red light and yellow light wave bands is realized; the probe provided by the invention has the advantages of high reaction rate, high selectivity and strong anti-interference capability, and can be used for dynamic ratio fluorescence imaging of peroxynitrite in cell and living animal models.