34 results about "Peroxynitrite" patented technology

The invention provides a probe for simultaneously detecting gamma-glutamyltranspeptidase and peroxynitrite and a preparation method of the probe. The probe provided by the invention can realize the effect of simultaneously detecting gamma-glutamyltranspeptidase and peroxynitrite, and has high sensitivity and specificity; meanwhile, the probe also has the advantages of low cytotoxicity and fast reaction. Therefore, the probe provided by the invention has potential application value in early diagnosis and in-situ monitoring of AKI, and is expected to become a powerful tool for evaluating kidney injury and oxidative stress states.

The present invention belongs to the field of biomedicine, and specifically relates to a light-controlled release of ONOO － Compounds and preparation methods and applications thereof. The present invention provides ONOO ‑ The donor is composed of a diphenyl phenothiazine analogue and arginine covalently linked. Its strong conjugation effect makes it extremely photoresponsive. The polymer has near-infrared light emission and excellent reactive oxygen and reactive nitrogen generation capabilities. When L6 is irradiated with a 660nm laser, it will produce superoxide anions and singlet oxygen. Singlet oxygen oxidizes the guanidine group of arginine to produce NO and ·O2 ‑ and NO to form peroxynitrite (ONOO ‑ ), achieving the goal of highly effective tumor cell killing. Therefore, this compound is a potentially highly effective photosensitizer for tumor killing. Compared to conventional photosensitizers, this killing method has stronger oxidative and nitrative cell damage capabilities, and its development cycle is shorter than that of other photosensitizers, thus possessing great potential application value in clinical treatment.

The invention discloses a dual-function fluorescent probe for simultaneously detecting viscosity and peroxynitrite as well as a preparation method and application of the dual-function fluorescent probe, and belongs to the technical field of fluorescent probes. According to the bifunctional fluorescent probe, naphthalimide and hemicyanine are connected through a carbon-carbon double bond, and viscosity and peroxynitrite are detected at the same time through double recognition sites. Due to free intramolecular rotation between naphthalimide and hemicyanine, an intramolecular charge transfer effect is twisted, and the probe is non-fluorescent. The fluorescence can be enhanced by two different ways: (i) rotation is limited by increasing viscosity, thereby generating strong near-infrared fluorescence; and (ii) carbon-carbon double bonds are destroyed after peroxynitrite is added, so that an intramolecular charge transfer mechanism is caused, and the fluorescence ratio is obviously enhanced. The probe has the advantages of good selectivity, rapid response and the like when being used for detecting viscosity and peroxynitrite, and has a huge application prospect in the technical fields of analytical chemistry, life science, biomedical treatment and the like.

The invention provides a fluorescent probe as well as a preparation method and application thereof, and the structural formula of the fluorescent probe is as follows: # imgabs0 #. The preparation method comprises the following steps: stirring and mixing 7-N, N-diethylamino-2H-chromene-3-aldehyde, brominated 4-methyl-N-(4 '-boronic acid pinacol ester benzyl) pyridine, a catalyst and a solvent, heating, and carrying out reflux reaction, so as to obtain 7-N, N-diethylamino-2H-chromene-3-aldehyde-brominated 4-methyl-N-(4'-boronic acid pinacol ester benzyl) pyridine; and then carrying out suction filtration and recrystallization to obtain a fluorescent probe product. The probe has good water solubility and biocompatibility and is positively charged. The color of the solution is amaranth, the absorption peak is located at 540 nm, the fluorescence peak is located at 720 nm (near-infrared region), and the fluorescent probe can be used for near-infrared region biological fluorescence imaging. The probe has the property of realizing ratio colorimetry and ratio fluorescence response to peroxynitrite. After the probe reacts with peroxynitrite in a CH3CN-pbs (v / v = 1: 1, v / v, pH = 7.4) solution, the color of the probe solution becomes yellowish from amaranth, A < 410 > nm / A < 540 > nm and the concentration of peroxynitrite are in a linear relation within the range of 0-10 [mu] M, and the detection limit is 95 nM. The red fluorescence of the probe is gradually weakened at 720nm, the bright yellow fluorescence of the probe is gradually enhanced at 568nm, the I568nm / I720nm and the peroxynitrite concentration are in a linear relationship in a range of 0-16 [mu] M, and the detection limit is 10nM. The probe has the advantages of high sensitivity, low detection limit and small background fluorescence interference.

The present invention provides a fluorescent probe and its preparation method and application. The structural formula of the fluorescent probe is as follows: The preparation method is: 7-N,N-diethylamino-2H-chromene-3-aldehyde, 4-methyl-N-(4'-boronic acid pinacol benzyl) pyridine bromide, a catalyst and a solvent are stirred, mixed and heated under reflux for reaction, and then filtered and recrystallized to obtain a fluorescent probe product. The probe has good water solubility, biocompatibility and is positively charged. The solution color is purple-red, the absorption peak is at 540nm, and the fluorescence peak is at 720nm (near-infrared region), which can be used for near-infrared biological fluorescence imaging. The probe has the property of realizing ratiometric colorimetry and ratiometric fluorescence response to peroxynitrite. After reacting with peroxynitrite in a CH3CN-pbs (v / v=1:1, v / v, pH=7.4) solution, the color of the probe solution changes from purple-red to slightly yellow, A 410nm / A 540nm The probe has a linear relationship with the concentration of peroxynitrite in the range of 0 to 10 μM, with a detection limit of 95 nM. The red fluorescence of the probe gradually weakens at 720 nm and the bright yellow fluorescence gradually increases at 568 nm. 568nm / I 720nm The probe has a linear relationship with peroxynitrite concentration in the range of 0 to 16 μM, with a detection limit of 10 nM. The probe has the advantages of high sensitivity, low detection limit, and low background fluorescence interference.

The application discloses a dual-response fluorescent probe and a preparation method and detection application thereof, and belongs to the technical field of fluorescent probes. The probe is prepared by condensation reaction of 4-methyl-7-hydroxy-8-aldehyde coumarin and 4-morpholine aniline, and can simultaneously and specifically respond to peroxynitrite and viscosity. ‑ The probe specifically reacts with ONOO to generate a concentration-dependent fluorescence enhancement, has high selectivity, fast response and good linearity, and the fluorescence is significantly enhanced with the increase of viscosity. The probe has lysosome targeting properties, and can realize real-time imaging and dynamic monitoring of endogenous and exogenous ONOO ‑ in living cells, and presents a high-contrast fluorescent signal in a non-alcoholic fatty liver model mouse liver tissue slice, so that ONOO ‑ overproduction and viscosity increase in a pathological state can be accurately identified.

The present invention discloses a method for detecting ONOO ‑ The near-infrared fluorescent probe and its preparation method and application belong to the field of peroxynitrite ion detection technology. ‑ The near-infrared fluorescent probe has a fast detection speed and can reach the highest fluorescence intensity within 60 seconds; the reaction is obvious and easy to observe. ‑ After the reaction occurs, strong red fluorescence is generated, and the color change of the solution can be observed with the naked eye; it has strong selectivity and high sensitivity, which can effectively avoid interference from other biological molecules and active substances, ensuring the accuracy and reliability of the detection results, and the minimum detection limit can reach 107nM; it has good biocompatibility and low toxicity in the body, and will not adversely affect the normal physiological functions of biological samples; the preparation method provided is simple and easy to operate, the raw materials are low in cost and readily available, the synthesis efficiency is high, it can be used for large-scale industrial production, and has broad application prospects.

This invention discloses a fluorescent probe for peroxynitrite, its preparation method, and its application, belonging to the field of analytical detection technology. The probe uses 4-bromo-1,8-naphthalenedicarboxylic anhydride and 4-(2-aminoethyl)morpholine as starting materials, and through a multi-step reaction, connects a 4-phenylboronic acid pinacol ester responsive group to obtain a benzisoquinoline-based fluorescent probe. The probe is a yellow solid, readily soluble in dimethyl sulfoxide, and exhibits no fluorescence before reacting with peroxynitrite. After the reaction, it emits strong yellow fluorescence at 575 nm under 500 nm excitation. The fluorescence intensity increases with increasing peroxynitrite concentration, with a detection limit as low as 0.13 μM. It exhibits high selectivity and good photostability. This probe can achieve real-time fluorescence imaging of endogenous peroxynitrite in hepatocytes and liver tissue of mice with acute liver injury. It can specifically identify peroxynitrite and is unaffected by thiols, metal ions, common enzymes, and other reactive oxygen species / reactive nitrogen species. It provides a highly sensitive and specific novel detection tool for the early diagnosis and molecular mechanism research of acute liver injury, and has significant scientific research and clinical application value.

The invention discloses a fluorescent probe for detecting peroxynitrite ions as well as a preparation method and application thereof, and relates to the technical field of biomedical detection, and the structural formula of the fluorescent probe is shown in the specification. The prepared fluorescent probe for detecting the peroxynitrite ions has high selectivity and high sensitivity on the peroxynitrite ions, the response speed is high, fluorescence stability can be achieved within about 30 minutes, ratio type fluorescent quantitative detection of the peroxynitrite ions in an aqueous solution is achieved, the linear correlation is good, and the detection sensitivity is high. The rapid detection of the peroxynitrite ion level in an oxidation modified low-density lipoprotein induced atherosclerosis cell model is also realized, and good biocompatibility and application potential are realized.

The invention provides a tumor-targeting nanometer generator for optically-controlled chemical generation of peroxynitrite and a preparation method and application thereof.The tumor-targeting nanometer generator for optically-controlled chemical generation of peroxynitrite comprises an inner core and a coating layer coating the inner core, the inner core comprises BNN-6-loaded Prussian blue, and the coating layer comprises a BNN-6-loaded Prussian blue and a BNN-6-loaded Prussian blue. The coating layer comprises a tumor cell membrane. According to the present invention, the specific generation of ONOO <-> on tumor cells under the irradiation of the near-infrared I region light can be achieved, the activity of pyruvate kinase (PK) and glutaminase (GLS) can be inhibited, and the glycolysis and the glutamine metabolism of the tumor cells can be destroyed;

The invention belongs to the field of organic molecular fluorescent probes, and particularly discloses a molecular fluorescent probe for detecting peroxynitrite in a mouse brain and a preparation method of the molecular fluorescent probe. The structural formula of the molecular fluorescent probe capable of detecting peroxynitrite is shown in the specification. Compared with other probes, the probe is simple in synthesis method, suitable for industrial production, high in sensitivity, high in selectivity and good in biocompatibility in peroxynitrite detection, and capable of accurately detecting the content of peroxynitrite in cells and avoiding interference of other substances in the cells. The nitrite peroxide level change can be detected in a Parkinson caenorhabditis elegans model. The probe is remarkably characterized in that the probe can efficiently penetrate through the blood-brain barrier (BBB) of the mouse brain, high-sensitivity detection of peroxynitrite on the brain is achieved, and the probe has remarkable brain targeting characteristics. The invention provides a simple and effective method for researching the action mechanism of the peroxynitrite in the brain in the development of the disease course of the Parkinson patient. # imgabs0 #

Provided is a method for spraying a mist containing peroxynitrous acid, which can be used in a wide range of applications such as finger disinfection applications and indoor spraying applications, and which is capable of changing the peak value of the concentration of peroxynitrous acid contained in the mist and the half-decay time of the concentration decay in accordance with the applications. In the spraying method, peroxynitrous acid is continuously generated by a spraying device from a plasma-activated mist having a pH in the range of 2 to 5, the plasma-activated mist being generated from an aqueous solution containing a nitrite salt having a concentration in the range of 10 micromoles to 10 mmoles.

This invention discloses a fluorescent probe for detecting peroxynitrite in living cells and its synthesis method. The fluorescent probe comprises an amphiphilic group, 4-amino-2-methoxyphenol as a recognition unit, and a rhodamine derivative fluorophore, wherein the recognition unit can interact with ONOO. ‑ A specific oxidation reaction occurs, causing significant changes in fluorescence intensity and emission wavelength, thereby enabling real-time imaging and quantitative analysis of peroxynitrite in the cellular microenvironment. The fluorescent probe of this invention possesses high selectivity, strong anti-interference capabilities, and rapid response, making it suitable for the detection of ONOO at the live-cell level. ‑ Dynamic observation.

This invention provides a 2-pyridinone-based afterglow chemiluminescent molecule, its preparation method, and its application. The Chinese name of the afterglow chemiluminescent molecule is (…). E )-2-(2-(6-((4-boronic acid benzyl)oxy)-7-fluoro-2,3-dihydro-1 H -oxanthracene-4-yl)vinyl)-3,3-dimethyl-1-(4-(3-methyl-2-oxopyridine-1-(2-yl)- ... H )-butyl)-3 H -Indole-1-onium. This invention also provides a method for preparing this afterglow chemiluminescent molecule. After being embedded in Pluronic F127 to form nanoparticles, the molecule exhibits excellent afterglow luminescence performance upon activation with oxynitrite in PBS buffer. This invention overcomes the challenges of irreversible substrate consumption, blue shift of emission, and low energy transfer efficiency in traditional organic afterglow systems by using 2-pyridone as a stable, non-consumable substrate and covalently linking it to the luminescent unit, providing a powerful new platform for precise molecular sensing and further disease diagnosis.

The invention belongs to the field of biomedical materials, and particularly relates to an NO slow-release dressing and a preparation method thereof. The preparation method comprises the following steps: firstly, incubating MIL-100 (Fe), amino-polyethylene glycol 3400-polyarginine and inducible nitric oxide synthase (NOS2) recombinant protein together, then dispersing solid particles into a sodium alginate solution, and cross-linking with calcium ions to prepare alginic acid hydrogel, namely the NO slow-release dressing. The dressing not only can realize antibiosis by generating peroxynitrite, but also can generate a proper amount of NO to stimulate cell proliferation, enhance synthesis and deposition of collagen, promote angiogenesis and accelerate wound healing.

The present invention discloses a fluorescent probe for dual detection of superoxide anions and peroxynitrosyl ions by targeting the Golgi apparatus, and its preparation method and application, belonging to the technical field of active oxygen probes. The fluorescent probe of the present invention has the molecular formula: C 42 H 54 F3N2O5S + The structural formula is: After the probe reacts with superoxide anions, the fluorescence intensity at 720 nm increases. Subsequently, after reacting with peroxynitrosyl ions, the fluorescence at 720 nm decreases and the fluorescence at 460 nm increases. The probe can also be used to detect superoxide anions and peroxynitrosyl ions in the Golgi apparatus of living cells, generating fluorescence images using confocal fluorescence microscopy. This suggests that the probe has potential practical value.

The invention discloses a rhodamine structure-based ONOOfluorescent probe as well as a preparation method and application thereof, a rhodamine skeleton is taken as a fluorescent chromophore, and free rotation of an aromatic amine unit is limited by constructing a rigid conjugate plane, so that the fluorescence quantum yield and the signal stability are improved; a quaternary ammonium salt type side chain is introduced to a rhodamine skeleton, and a cationic group is used for enhancing water solubility; an aryl borate recognition group is connected to a rhodamine parent nucleus through an aromatic bridging unit, and when ONOO is subjected to specific oxidation, a spontaneous deprotection process is carried out, so that ring opening of rhodamine and'lightening 'of fluorescence are triggered. The preparation method of the peroxynitrite fluorescent probe based on the rhodamine structure, disclosed by the invention, has the advantages of simple steps, mild conditions, easily available raw materials and higher yield, and is suitable for large-scale industrialization. The reagent is used as a high-sensitivity quantitative detection reagent for ONOO in a solution system; the fluorescent probe is used for fluorescence imaging monitoring of ONOO in living cells; the method is used for ONOO living body imaging and early evaluation in a drug-induced liver injury animal model.

The application discloses a hyaluronic acid modified co-loaded all-trans retinoic acid and arginine liposome and a preparation method and application thereof, and belongs to the field of pharmaceutical preparations.The liposome components comprise phospholipid, cholesterol, hyaluronic acid, all-trans retinoic acid and arginine.The prepared liposome can generate nitric oxide in response to a large amount of active oxygen existing in a liver fibrosis microenvironment, the generated nitric oxide is further oxidized into peroxynitrite by ROS, matrix metalloproteinase is activated, an extracellular matrix barrier is reduced, and nanoparticles are promoted to penetrate into a perisinusoidal space of the liver.The liver stellate cells are targeted through receptor-ligand specific combination, and the uptake efficiency of the liver stellate cells is improved.The released all-trans retinoic acid in cells is converted into a resting state by inducing activated liver stellate cells, and the regression of liver fibrosis diseases is promoted.The liposome penetrates through an extracellular matrix to promote nanoparticle penetration, enhances drug delivery, and provides a new way and strategy for efficient delivery of therapeutic drugs.

Selective probes provide tandem activity-based sensing and labeling of reactive oxygen species (ROS), such as hydrogen peroxide, peroxynitrite, and organic peroxides.

The invention belongs to the field of chemical analysis and testing, and particularly relates to a resorufin fluorescent probe for specifically recognizing peroxynitrite (ONOO) based on 4-(trifluoromethyl) benzyl bromide and application of the resorufin fluorescent probe. Resorufin and 4-(trifluoromethyl) benzyl bromide are dissolved in dimethylformamide (DMF), and reaction liquid is subjected to a water bath reaction for 8-10 hours at the temperature of 50-60 DEG C under the alkaline condition of potassium carbonate. An obtained crude product is separated and purified through thin layer chromatography, and the resorufin fluorescent probe HRA-1 based on 4-(trifluoromethyl) benzyl bromide is obtained. The resorufin fluorescent probe HRA-1 based on 4-(trifluoromethyl) benzyl bromide can realize high-sensitivity and high-specificity fluorescent response to peroxynitrite (ONOO-) in a dimethyl sulfoxide (DMSO) solution, the response time is short, and the operation is simple and convenient.

The present invention discloses a dual-response fluorescent probe, a preparation method and an application thereof, and relates to the technical field of small molecule probes. The chemical structure, preparation method and application of the dual-response fluorescent probe are provided. The background fluorescence of the fluorescent probe P2 prepared by the present invention itself is negligible. If the probe is in a high-viscosity medium, it can emit near-infrared fluorescence at 819nm, thereby realizing viscosity detection; when peroxynitrite is present in the system, the conjugated structure of the probe is destroyed, and the probe emits green fluorescence at 511nm, thereby achieving the recognition of peroxynitrite. Cell experiments found that the probe can be used for fluorescent imaging of intracellular viscosity and peroxynitrite. The probe can also be used for viscosity imaging analysis of inflammatory and diabetic mice. In addition, we can simultaneously monitor changes in viscosity and peroxynitrite levels during ferroptosis.

The application provides a probe for simultaneously detecting gamma-glutamyl transpeptidase and peroxynitrite and a preparation method thereof. The probe can realize the effect of simultaneously detecting gamma-glutamyl transpeptidase and peroxynitrite, has high sensitivity and specificity, and has the advantages of low cytotoxicity and fast reaction. Therefore, the probe has potential application value in early diagnosis and in-situ monitoring of AKI, and is expected to become a powerful tool for evaluating kidney injury and oxidative stress.

The invention relates to a multi-response type nano gas therapeutic agent as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The gas therapeutic agent comprises MnCO nanoparticles coated with a PLGA-SNAP polymer, the MnCO nanoparticles can respond to GSH and H2O2 which are overexpressed in tumor cells to generate double gases (NO and CO), and NO and CO can effectively induce mitochondrial damage through a synergistic effect and generate peroxynitrite (ONOO-) through a cascade reaction to further induce cell nucleus damage. Meanwhile, mtDNA and gDNA released by damaged mitochondria and cell nucleuses can activate cGAS / STING pathways in DCs cells, induce the cGAS / STING pathways to be mature and stimulate secretion of IFN-beta, and start of effector T cells in the tumor microenvironment is enhanced. In addition, the gas therapeutic agent does not cause side effects on normal cells or tissues.

The invention relates to the technical field of fluorescent probes, and particularly discloses a peroxynitroso ion and viscosity difunctional fluorescent probe based on a cyanopyridine structure as well as a preparation method and application of the peroxynitroso ion and viscosity difunctional fluorescent probe based on the cyanopyridine structure, and the probe is formed by connecting cyanopyridine salt serving as a strong electron withdrawing group and naphthyl methyl ether serving as an electron donating group through an unsaturated C = C bond. When the viscosity changes, the probe realizes green fluorescence response to the viscosity by regulating and controlling a T-ICT mechanism. In the presence of ONOO <->, a C = C bond is subjected to oxidative cleavage, and a blue fluorescence signal is released, so that quantitative detection of ONOO <-> is realized. The probe has the advantages of large Stokes shift, high response speed, strong anti-interference capability, simple and efficient preparation method and the like, is successfully used for dual-channel fluorescence imaging of ONOO <-> and viscosity in cells, and provides an effective detection tool for researching a correlation mechanism of oxidative stress and microenvironment change.

The invention discloses targeted nano-liposomes (SPL NPs) capable of releasing nitric oxide (NO) through ultrasonic triggering and application of the targeted nano-liposomes (SPL NPs) combined with a PD-L1 antibody in pancreatic cancer treatment. The nano-liposome is formed by self-assembly of DSPE-PEG2000-SNO (which is used as a NO prodrug), pyropheophorbide acid (PPAL (which is used as a sound-sensitive agent) and DSPE-PEG2000-LFC131 (which is used as a pancreatic cancer targeting ligand), and has good stability and targeting property. Under an ultrasonic irradiation condition, the nano system can generate active oxygen at a tumor part through the sonodynamic effect of PPAL, and can induce obvious immunogenic cell death. In addition, quick release of NO of an NO prodrug DSPE-PEG2000-SNO at a tumor site can be induced, and peroxynitrite with higher activity is generated through combined action of active oxygen and NO, so that an efficient tumor cell killing effect is realized. Meanwhile, the released NO can effectively release a pancreatic cancer compact matrix, improve the tumor microenvironment and enhance the permeability of drugs and the infiltration capacity of immune cells. The invention also discloses an application of the nano-liposome system in pancreatic cancer treatment.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a fluorescent probe for sequentially activating and detecting hydrogen sulfide and peroxynitrite as well as a preparation method and application of the fluorescent probe. The structural formula of the fluorescent probe is shown as a formula (I). When the # imgabs0 # reacts with hydrogen sulfide, the sulfonic acid bond of the # imgabs0 # can be broken by H2S to generate a red fluorescent substance. When the peroxynitrite is continuously added, the peroxynitrite can selectively oxidize the carbon-carbon double bond of the red fluorescent substance to generate a blue fluorescent substance, so that sequential activation detection is realized. The fluorescent probe provided by the invention has the advantages of double-recognition function, high sensitivity, low detection limit, high selectivity, low biotoxicity and the like. In addition, the probe can sequentially activate and detect exogenous and endogenous hydrogen sulfide and peroxynitrite of cells and organisms, and has wide application prospects in the technical fields of analytical chemistry, life science, biomedical treatment and the like.

The present invention relates to an artificial cornea capable of light-controlled release of nitric oxide, and belongs to the field of medical devices. A carboxymethyl chitosan and sodium alginate gel coating is prepared on the surface of the artificial cornea, and the coating contains liposome nanoparticles encapsulating NO donors, and the NO donors can release NO in response to light, and play the role of regulating intraocular pressure, antibacterial and anti-inflammatory. At the same time, EDC and NHS are used to connect metal porphyrins as a peroxynitrite scavenging layer to remove highly toxic peroxynitrite produced by the reaction of NO with endogenous superoxide anions, and prevent the damage of peroxynitrite caused by NO release. The artificial cornea can reduce complications such as glaucoma, infection and inflammation caused by artificial corneal transplantation.

A method for preparing chiral silicon quantum dots for detecting peroxynitrite concentration, which relates to a method for preparing chiral silicon quantum dots. The present invention aims to solve the problem that the fluorescence of melanin is partially quenched by aqueous solution, resulting in a low fluorescence intensity; solve the problem that existing SiQDs cannot detect ONOO ‑ The specific detection is carried out, and the emission range, fluorescence lifetime and quantum yield are low, which makes it impossible to fully utilize the emission light of quantum dots, resulting in poor detection effect and the inability to achieve endogenous detection of cells. Method: 1. Preparation of chiral melanin particles (K / P) by oxidation method ox Solution; 2. Preparation of SO3‑(K / P) by stirring method ox ;3. Preparation of SiQDs-(K / P) by hydrothermal method ox The present invention is used to prepare chiral silicon quantum dots for detecting the concentration of peroxynitrite.

The invention belongs to the technical field of fluorescent sensing detection, and particularly relates to an indole sulfonic acid-phenothiazine fluorescent probe as well as a preparation method and application thereof. The structural formula of the indole sulfonic acid-phenothiazine fluorescent probe is shown in the specification. The probe has a remarkable fluorescence enhancement change on peroxynitrite in a DMSO / water (7 / 3) solution (10mM, pH 7.4), is rapid in detection response (within 5 minutes), low in detection limit (29.19 nM) and excellent in detection selectivity, and is applied to fluorescence imaging detection of exogenous and endogenous peroxynitrite in living cells.

The application discloses a ratio fluorescent probe for detecting peroxynitrite and a preparation method and use method thereof. The application uses (E)-2-(3-(4-aminostyryl)-5,5-dimethylcyclohexyl-2-en-1-alkyl malonitrile to construct a classic intramolecular charge transfer system. When peroxynitrite exists, the peroxynitrite and an amide part on the probe molecule are oxidized to break and generate a strong electron donor-amino, and then the ICT effect of the molecule is enhanced, the emission wavelength is red-shifted, and the probe molecule emits strong red fluorescence. The ratio peroxynitrite probe of the dicyanisophorone dye provided by the application has good response performance to a peroxynitrite solution, can realize sensitive, rapid and quantitative detection of trace peroxynitrite in a sample, and has the advantages of simple operation, low cost, good water solubility and sensitive response.