Nanoparticles capable of controllably preparing ONOO<-> as well as preparation method and application of nanoparticles

A technology of nanoparticles and reaction solution, applied in the field of controllable preparation of ONOO- nanoparticles and preparation thereof, can solve the problems of large deviation of production efficiency and increased operation complexity, etc.

Active Publication Date: 2021-12-24
WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This increases the complexity of the operation, and due to factors such as the sequence of operations of different stimuli and the individual differences of operators, it will cause ONOO ─ The production efficiency has a large deviation

Method used

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  • Nanoparticles capable of controllably preparing ONOO&lt;-&gt; as well as preparation method and application of nanoparticles
  • Nanoparticles capable of controllably preparing ONOO&lt;-&gt; as well as preparation method and application of nanoparticles
  • Nanoparticles capable of controllably preparing ONOO&lt;-&gt; as well as preparation method and application of nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: 5 g of polyethylene glycol monomethyl ether (Mn = 5000) was dissolved in 50 mL of anhydrous dichloromethane, and 202 mg of triethylamine was added. Under ice-bath conditions, 460 mg of bromoisobutyryl bromide was slowly added dropwise to the mixture. Under rapid stirring, react for 24 hours. The reaction solution was washed with saturated brine, extracted with dichloromethane, precipitated with n-hexane (500 mL, 3 times), filtered with suction, and dried in vacuo to obtain 5.1 g of PEG-Br sample. Adopt nuclear magnetic resonance spectrometer to test gained PEG-Br sample, test result is as follows figure 2 shown.

Embodiment 2

[0056] Embodiment 2: Take PEG-Br sample 2 g and dissolve in 10 mL of N, N - In dimethylformamide (DMF), add 2.5 g of tert-butyl acrylate, 135 mg of pentamethyldiethylenetriamine (PMDETA), and add 112 mg of cuprous bromide under nitrogen protection. Under rapid stirring, heat in an oil bath at 65°C for 24 hours. Pass through a neutral alumina column three times to remove metallic copper in the reaction solution, then dialyze with ultrapure water for 48 hours, and freeze-dry to PEG- b - PtBA sample. The obtained PEG- b -PtBA samples were tested and the test results were as follows image 3 shown.

Embodiment 3

[0057] Embodiment 3: take PEG- b - Dissolve 2 g of PtBA sample in 30 mL of dichloromethane, and add 5 mL of trifluoroacetic acid. Subsequently, under rapid stirring, the reaction was carried out for 48 hours. Spin to remove most of the solvent, and then precipitate three times with glacial ether to obtain PEG- b - PAA samples. The obtained PEG- b -PAA samples are tested, the test results are as follows Figure 4 shown.

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Abstract

The invention relates to nanoparticles capable of controllably preparing ONOO<-> as well as a preparation method and application of the nanoparticles. Novel photosensitizer PEG-b-PAA-g-SNO@IR780 nanoparticles are designed and prepared based on the synergistic effect between nitric oxide and photodynamic therapy, and simple, efficient and controllable release of ONOO<-> is achieved based on single 808 nm laser stimulation. Under irradiation of single 808 nm laser, simple, efficient and controllable release of ONOO<-> is realized under single near-infrared light stimulation, and the nanoparticles are expected to provide a novel tool for simple, efficient and controllable release of peroxynitrite, and are used for exploring in-situ release of the peroxynitrite and physiological and pathological interaction thereof and used in the fields of antibacterial and anti-tumor treatment.

Description

technical field [0001] The invention relates to the technical field of controlled release of peroxynitrite, in particular to a controllable preparation of ONOO - Nanoparticles and their preparation methods and applications. Background technique [0002] Peroxynitrite is a short-lived endogenous substance. It plays a vital role in physiological and pathological processes such as inflammatory response, cancer, cardiovascular disease, and neurodegenerative diseases. Due to the strong oxidation and nitrification activity (higher than nitric oxide, singlet oxygen, hydroxyl radicals, etc.), overexpressed peroxynitrite in organisms irreversibly damages biological molecules such as proteins, nucleic acids, and liposomes. destruction. In recent years, a small number of pioneering scientific works have shown that the exogenous release of ONOO — The nanomaterials have shown good therapeutic effects in both antitumor therapy and antibacterial therapy. However, simple, efficient and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K33/40A61K9/51A61K47/60A61P35/00A61P31/04A61P31/10
CPCA61K41/0057A61K33/40A61K9/5146A61P35/00A61P31/04A61P31/10A61K2300/00Y02A50/30
Inventor 石长灿姜大伟潘璐琪杨啸季志孝李徐坚
Owner WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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