The invention relates to an up-conversion fluorescent / magnetic nanomaterial-labeled immune-chromatographic test paper and a making method thereof. The immune-chromatographic test paper is constructed on the basis of optimizing the structure and all composition parts of a test strip through combining the fluorescent and magnetic characteristics of an up-conversion fluorescent / magnetic material with an immune-chromatographic technique. The immune-chromatographic test paper comprises a support material and an upper layer, and the upper layer comprises a sample pad, a binding and releasing pad, a 
cellulose membrane and a water absorbing material, wherein the 
cellulose membrane has a detection line and a 
quality control line, the detection line is coated with an 
antigen or 
antibody of an object to be measured, the 
control line is coated with a secondary 
antibody, and the binding and the releasing pad is loaded with an up-conversion fluorescent / magnetic nanomaterial-labeled 
antibody which can be bond to the object to be measured. The invention realizes the fluorescent quantitative and potentially magnetic quantitative detection of analytes in the fields of 
drug residual and the like based on the up-conversion fluorescent / magnetic nanomaterial-labeled immune-chromatographic technique, and can significantly improve the detection sensitivity of a traditional 
colloidal gold-labeled immune-chromatographic 
analysis method. The above test strip has the advantages of good specificity, high sensitivity, simple, intuitive and accurate detection, low cost, wide application range, and easy popularization and application.