100results about How to "Realize quantitative detection" patented technology

The invention provides a single-particle transient pulse detection circuit in a nanometer integrated circuit, and relates to the technical field of single-particle transient effect detection, and the circuit comprises a chain-type to-be-detected circuit, a single-particle transient pulse capture circuit and a single-particle transient pulse number counting circuit. Wherein the single-particle transient pulse capture circuit and the chain type circuit to be detected are tightly coupled circuits, the single-particle transient pulse capture circuit comprises a plurality of D-type latches with reset, and when the width of a single-particle transient pulse is larger than the propagation delay time of the buffer, the single-particle transient pulse is captured and stored by the D-type latches; therefore, the width of the single-particle transient pulse is detected. The single-particle transient pulse number counting circuit comprises a plurality of D-type trigger groups with reset and a counter group; when the D-type trigger group detects a single-particle transient pulse signal entering the input end of the D-type trigger group, a counting signal is input to the counter group, and counting is completed by the counter group. According to the invention, specific quantitative detection of the single-particle transient effect can be realized.

The invention discloses a method for extracting a p-phenylenediamine antioxidant in a liquid sample and application of the p-phenylenediamine antioxidant. The p-phenylenediamine antioxidant comprises N-(1, 2, 4-trimethyl-1, 3-pentanediol) the organic solvent is at least one of N, N '-diphenyl-1, 3-dimethyl butyl)-N'-phenyl p-phenylenediamine, N-cyclohexyl-N '-phenyl p-phenylenediamine, N-isopropyl-N'-phenyl-p-phenylenediamine, N, N '-diphenyl-1, 4-phenylenediamine, N, N'-di-2-naphthyl p-phenylenediamine and 2-((4-methylpentane-2-yl) amino)-5-(phenyl amino) cyclohex-2, 5-diene-1, 4-diketone. According to the method, liquid-liquid extraction which is simple and efficient to operate, low in cost and good in repeatability is adopted as a sample pretreatment method, and is combined with an isotope label method and an ultra-high performance liquid chromatography-tandem mass spectrometry technology, so that separation and qualitative and quantitative detection of six p-phenylenediamine antioxidants and quinone derivatives in a sample are realized; meanwhile, the sensitivity is high, the quantity of isotope internal standards is small, the solvent loss is small, and the actual detection requirement is met.

The invention discloses a self-powered sensor for detecting sugarcane smut pathogenic bacteria and a preparation method thereof, the self-powered biosensor comprises an anode, a cathode and an electrolyte, the anode is an Au / N-GDY / GOD / BSA biological anode, the cathode is an Au / N-GDY / DNA chain biosensor, and the electrolyte comprises glucose, [Ru (NH3) 6] 3 + and a PBS buffer solution. The sensor provided by the invention is used for portable detection of pathogenic bacteria of sugarcane smut, and quantitative analysis of a target analyte is realized through change of power output of the enzyme biological fuel cell. The target object can be simply, quickly, sensitively and efficiently detected.

The invention discloses an immunodetection kit based on a bimetal nano-bomb biological probe, and relates to the technical field of gene detection, the immunodetection kit comprises a test strip and an immunoprobe, the immunoprobe is a suspension of a bimetal nano-bomb and a monoclonal antibody, and the mixing ratio of the bimetal nano-bomb to the monoclonal antibody is 0.1-0.3 mg: 5 [mu] g. The bimetal nano-bomb has a symmetrical hollow nano-bomb structure, so that the bimetal nano-bomb has excellent colorimetric and photo-thermal properties, and the detection sensitivity of salmonella typhimurium is greatly improved.

The invention relates to a kit and a method for simultaneously detecting IL-6, IL-21 and T cell CD4 surface molecules in lymphatic tissue, and belongs to the field of biomedicine. The kit comprises a quantum dot marked with an IL-6 antibody, a quantum dot marked with an IL-21 antibody and a quantum dot marked with a CD4 antibody. The antibodies marked by the quantum dots are specifically combined with antigens in lymphatic tissues respectively; the CD4T cells have the capacity of secreting IL-6 and IL-21, and the mutual relation between the CD4T cells and IL-6 and IL-21 reflects the tissue immunocompetence. The invention provides a kit for simultaneously detecting IL-6, IL-21 and T cell surface molecules in lymphatic tissues, which positions the spatial relationship between two cell factors and T cells, calculates and analyzes corresponding fluorescence intensity and evaluates immune conditions. The kit has the advantages of high fluorescence intensity, accurate detection result, wide spectrum selection, wide applicability, stable fluorescence structure, long preservation time, high sensitivity, good specificity and the like.

The invention discloses application of a ratiometric fluorescence sensor based on a fluorescein isothiocyanate labeled metal organic framework in escherichia coli detection. The method comprises the following steps: synthesizing ZTMs by taking TPPS as a ligand, and then fully incubating with FITC to prepare ZTMs (at) FITC; according to the present invention, Escherichia coli is used to reduce Cu < 2 + > to Cu < + >, such that the fluorescence intensity of ZTMs (at) FITC is changed, the fluorescence intensity of the emission peak at the 515 nm position is reduced, the fluorescence intensity of the emission peak at the 683 nm position is increased, and the color of the solution is observed to gradually change from yellow to orange and finally to red under the 365 nm ultraviolet light; and rapid and sensitive detection of the escherichia coli is realized through the ratio of the fluorescence intensities of the two emission peaks. According to the detection method, the accuracy of a detection result is improved, errors caused by environment, instruments and manual operation can be avoided, professional operators are not needed, the method has the advantages of being rapid, simple and independent of expensive instruments and equipment, and the detection cost is reduced.

The invention discloses a method for detecting homocysteine by a nano-pore sensor based on silver-sulfur polymerization, which comprises the following steps: pretreating a solution containing the homocysteine by 4-dimethylamino cinnamyl aldehyde, and then putting a sensor with a cylindrical nano-pore structure into the solution containing the homocysteine to carry out polymerization reaction; establishing a linear equation of the ratio of the conductivity before and after the polymerization reaction and the concentration of the homocysteine, and calculating the concentration of the homocysteine in the liquid to be detected through the linear equation. The liquid to be detected contains homocysteine and cysteine, and is pretreated by 4-dimethyl amino cinnamyl aldehyde before detection; and an Ag (I) probe is modified in a cylindrical nanopore channel of the sensor. According to the method, a special pretreatment method is utilized to cooperate with a constructed functional probe and a special nanopore structure, and current signal change is amplified based on silver-sulfur polymerization reaction, so that rapid and accurate detection of Hcy is realized.

The invention discloses a primer group and a kit for detecting spinal muscular atrophy related genes and a kit using method, the primer group comprises an amplification primer group and an extension primer group, primer nucleotide sequences in the amplification primer group are as shown in SEQ ID NO: 1-14, and primer nucleotide sequences in the extension primer group are as shown in SEQ ID NO: 15-26. The kit provided by the invention comprises the primer group, a PCR reaction reagent, an SAP reaction reagent and an extension reaction reagent, and can simultaneously detect SMN1 and SMN2 gene copy numbers, 7 hotspot mutations in people and c.* 3 + 80Tgt closely related to silent carriers in one reaction; the qualitative detection of the G site has a wider detection range for spinal muscular atrophy. Meanwhile, the amount of sample templates required by nucleic acid detection of the kit is small, the detection limit of the nucleic acid templates can be as low as 1 ng / mu L, and the kit has good sensitivity, specificity, stability and accuracy and can meet the current needs of screening patients with spinal muscular atrophy and silent carriers and prenatal diagnosis of infants.

The utility model provides an air tightness detection device for a copper bar injection molding part, and the device comprises a fixed plate, the fixed plate is provided with a sealing part, the sealing part is provided with two sealing cavities matched with a sealing detection part of the copper bar injection molding part, the interiors of the two sealing cavities are communicated, and one side of the sealing part is provided with an air source device communicated with the sealing cavities; the method has the advantages of being high in detection efficiency, more sensitive to tiny leakage, wider in application range, capable of achieving quantitative detection, low in use cost, operation cost and maintenance cost, easy to operate, low in environment requirement and the like.

The present invention relates to the field of electrochemical detection technology, and relates to an Ag3PO4-UiO-66 / apt / GO / GCE electrochemiluminescence sensor and its application. The sensor comprises a glassy carbon electrode, the outer surface of which is modified in sequence by Ag3PO4-UiO-66 as a resonance energy donor, apt, a ligand adapted to an object to be detected, and GO as a resonance energy receptor, and Ag3PO4-UiO-66 and GO are connected by apt; Ag3PO4-UiO-66 is a UiO-66 material loaded by Ag3PO4. The sensor has the advantages of high detection sensitivity, fast detection speed, strong specificity, wide linear range, and easy use.

The present invention provides a magnetically controlled fish pathogenic microorganism microfluidic chip detection platform with an ultra-smooth surface and a method of use, which belongs to the field of microbial detection technology. The microfluidic chip detection platform described in the present invention is an application of the integration of three research direction technologies: super-hydrophobic anti-biological adhesion materials, magnetically controlled separation and sorting, and electrochemical microbial Coulter counting detection sensors. The super-hydrophobic anti-biological adhesion material can increase liquid flux and avoid sample residue. The present invention combines immune nano-magnetic beads with magnetically controlled sorting functions to improve sorting efficiency; the present invention adopts a labeled fish pathogenic microorganism Coulter counting technology, and uses a Coulter counting method that generates instantaneous fluctuation pulses of current due to conductivity changes to achieve quantitative detection of fish pathogenic microorganisms. Compared with traditional laboratory incubation methods, microscope counting methods, etc., it has the advantages of less sample consumption and shorter time consumption.

The invention relates to a method for constructing a label-free immune platform through enzyme / antibody non-covalent modification of ZnNFs and ZnNPs, and belongs to the technical field of nano biosensing. The method comprises the following steps: respectively synthesizing ZnNFs and ZnNPs under the conditions of 25 DEG C and 4 DEG C based on a zinc nitrate and 3-methyl-1H-1, 2, 4-triazole (Hmtz) one-pot synthesis method, and constructing a completely non-labeled enzyme-linked immunosorbent assay platform to realize the detection of cardiac troponin I. The ZnNFs and the ZnNPs have the advantages of simple synthesis, uniform size, strong adsorption capacity and the like, the newly constructed magnetic separation complete non-labeled enzyme-linked immunosorbent assay platform successfully realizes the detection of cardiac troponin I and new coronavirus N protein in a complex environment, and the standard recovery rate experiment is within the standard error range. The sensing platform has huge potential in the aspect of disease diagnosis.

The embodiment of the invention provides a chemiluminescence immunodetection method and system combined with an electroluminescent compound label, and belongs to the technical field of data processing. The method comprises the following steps: performing optical stimulation on a target compound corresponding to a target antigen by using a photoelectric detector to obtain a target stimulation result; wherein the target compound is obtained by combining an electroluminescent compound with an initial compound, and the initial compound is obtained by combining a primary antibody with a target antigen; performing image acquisition on the target stimulation result by using an image sensor to obtain an initial optical image corresponding to the target antigen; performing image processing on the initial optical image to obtain a target optical image; performing image segmentation on the target optical image to obtain a target area, and determining brightness intensity corresponding to the target area according to the target optical image; and determining the antigen concentration corresponding to the target antigen according to the brightness intensity.

The invention relates to the technical field of durability detection, in particular to an automobile lightweight pedal assembly durability detection device which is characterized in that a box base is fixedly connected to the interior of an outer box body, a rotating motor is mounted at the top of the box base, and a rotating gear is fixedly connected to the output end of the rotating motor; an installation top ring is fixedly connected to the top of the outer box body, an installation top plate is fixedly connected to the inner side of the installation top ring, and an inner gear ring is fixedly connected to the bottom of the installation top plate. According to the pedal assembly durability testing device, simulation of different treading forces is achieved, the durability of the pedal assembly is comprehensively tested, meanwhile, the treading end is located between the two pushing devices by adjusting the height, and a denser treading mode and a mode combining heavy treading and light treading are achieved.

The present invention relates to a power cable extruded semi-conductive shielding sharp corner detection and evaluation system and method thereof. The present invention constructs an evaluation system including an image acquisition and display module, an arc coordinate positioning module, a sharp corner top coordinate positioning module and an evaluation calculation module. At the same time, the arc coordinate positioning module calculates the center and radius of the arc point set fitting, and the sharp corner top coordinate positioning module calculates the center and radius of the sharp corner top point set fitting. The evaluation calculation module quantitatively evaluates the semi-conductive shielding sharp corners to construct an evaluation method of the evaluation system. The present invention realizes the quantitative detection of the sharp corners of the extruded semi-conductive shielding of power cables. At the same time, the digital level of power cable network access detection is improved, the problem of sharp corners in semi-conductive shielding is eliminated, and the network access and laying of cables are improved, thereby improving the safety and reliability of the power grid.

The present invention provides an IGBT defect detection method and device based on the gate current signal of the turn-on process, including obtaining the current waveform of the target stage when the IGBT is actually working based on the gate current signal of the IGBT turn-on process; calculating the gate capacitance charging charge of the target stage when the IGBT is actually working according to the current waveform of the target stage when the IGBT is actually working; and analyzing and judging whether the current IGBT has a gate-emitter loop defect according to the gate capacitance charging charge of the target stage when the IGBT is actually working. The present invention analyzes the change of the gate charging charge through the gate current waveform of the IGBT turn-on process, and then judges whether the IGBT has a gate-emitter loop defect, thereby improving the detection efficiency. When there is a defect in the IGBT, the technical solution of the present invention can also be used to know the number of packaged chips with specific defects in the IGBT, and quantitative detection can be achieved.

The utility model belongs to superconducting technical field discloses a kind of high-temperature superconducting tape wrapping and detection system, including pay-off device, cleaning device, wrapping device, pressing device, traction device, meter device, visual identification device and take-up device sequentially arranged along the output direction of superconducting tape, the high-temperature superconducting tape wrapping and detection system can effectively remove the dirt and metal chips and other pollutants on the surface of tape, pretreat before wrapping high-temperature superconducting tape, ensure insulation wrapping effect, and the stacking distance of insulating tape can be detected, the quantitative detection of insulation effect after superconducting tape wrapping is realized, which is beneficial to improve wrapping effect.

This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.

The invention provides an IGBT defect detection method and device based on a gate voltage signal in an opening process, and the method comprises the steps: obtaining a voltage waveform of a target stage during the actual work of an IGBT based on the gate voltage signal in the opening process of the IGBT; according to the voltage waveform of the target stage when the IGBT actually works, calculating to obtain the gate loop discharge time of the target stage when the IGBT actually works; and analyzing and judging whether the IGBT to be detected has gate and emitter loop defects or not according to the gate loop discharge time of the target stage during the actual work of the IGBT. According to the invention, the change of the discharge time of the gate loop is analyzed through the gate voltage waveform in the opening process of the IGBT, so that whether the IGBT has gate emitter loop defects is judged, and the detection efficiency is improved. When gate emitter loop defects exist in the IGBT, the number of packaging chips, specifically having the gate emitter loop defects, of the IGBT can be known by adopting the technical scheme of the invention, and quantitative detection can be realized.

The utility model discloses a linear machine for spring production relates to the field of steel wire straightening machine, the utility model discloses a linear machine main part, the detection component includes the mounting panel, the mounting panel is threadedly connected with the adjusting screw rod, the end of adjusting screw rod is rotatably connected with the moving frame through the thrust bearing, the moving frame is close to the one side of steel wire and is provided with the mounting frame, the pressure sensor is fixedly arranged between the mounting frame and moving frame, the utility model discloses a detection component, sets up four detection components with pressure sensor at the linear machine main part export, realizes the quantitative detection of steel wire straightening precision, and the system utilizes the contact of pressure wheel and steel wire, and the bending degree is converted into pressure signal, and the pressure sensor measures and transmits to the control screen display, replaces the traditional manual visual judgment, makes the detection result have objectivity and repeatability, and the quality consistency and control precision of spring production have been improved significantly.

The invention discloses application of a fomesafen specific fluorescent probe based on rhodamine skeleton fluorene. The fomesafen specific fluorescent probe can be used for detecting fomesafen in agricultural products, environmental samples and biological samples. The method specifically comprises the following steps: immersing test paper into a 40 [mu] M probe 4 solution for 1 h, airing at room temperature, cutting the test paper into a plurality of love hearts, placing the love hearts on a glass slide, dripping fomesafen with different concentrations on the test paper to form an experimental group, performing water treatment to form a control group, enabling liquid drops to completely cover the surface of the test paper, airing at room temperature, and determining the fomesafen content of the test paper. A fluorescent probe 4 is arranged on a paper strip, a fluorescent image of the paper strip is shot under irradiation of a 365 nm ultraviolet lamp in a natural light and dark environment, the fluorescent probe 4 shows excellent performance, the fluorescent probe 4 is integrated into test paper and hydrogel, and portable and instantaneous detection in an actual sample is facilitated in combination with a platform based on a smart phone. The method is a portable, simple and accurate method for detecting the fomesafen content in real time.

The invention discloses a preparation method and application of a high-stability hydrogen sulfide detection card, and the preparation method at least comprises the following steps: step I, dispersing dithizone in ethanol to obtain a dithizone solution; iI, dissolving lead acetate in water to obtain a lead acetate solution; iII, under the alkaline condition, mixing the dithizone solution obtained in the step I with the lead acetate solution obtained in the step II to obtain a dithizone-Pb complex; step IV, mixing the methanol solution containing tetrabutyl ammonium hydroxide, N, N-dimethylformamide, water and ethanol, and carrying out water bath reaction to obtain an alkaline silica gel solid carrier; v, dispersing the dithizone-Pb complex obtained in the step III into the alkaline silica gel solid carrier obtained in the step IV to obtain a dithizone-Pb complex solid carrier; and step VI, dispensing the dithizone-Pb complex solid carrier obtained in the step V on a thin film to obtain the hydrogen sulfide detection card. The detection card can be used for quantitative detection of hydrogen sulfide and drug sensitivity analysis of bacteria.

The invention provides a nitrite quantitative detection method based on surface enhanced Raman spectroscopy, relates to the technical field of surface enhanced Raman spectroscopy, and constructs a self-correcting SERS substrate through a three-phase liquid-liquid interface co-assembly technology. In the experiment process, a sample to be detected is mixed with a thiobarbituric acid solution, the mixture is adjusted to be acidic, NO2 <-> and thiobarbituric acid are subjected to an S-nitrosation reaction, and S-nitroso mercaptan is generated. And then, immersing the self-correcting SERS substrate into a reaction solution, so that the substrate adsorbs the generated S-nitroso mercaptan, and further, a Raman signal is enhanced. After soaking is completed, the substrate is taken out and dried, finally, a Raman signal is collected through a Raman spectrometer, and the concentration of NO2 <-> is accurately analyzed through the change of the signal intensity. According to the technical scheme, rapid and high-sensitivity detection of NO2 <-> is achieved, good selectivity and anti-interference capacity are achieved, and the method is widely suitable for detection requirements of multiple fields such as environment monitoring and food safety.

The invention discloses fluorescent hydrogel, a kit for detecting biogenic amines in aquatic products and application of the fluorescent hydrogel, and belongs to the technical field of biological detection.The fluorescent hydrogel comprises agar and nitrogen-sulfur co-doped carbon dots coated with manganese dioxide nanoflowers, the fluorescent hydrogel is matched with a DAO solution and an OPD solution to achieve visual detection of biogenic amines in aquatic products, the invention further provides a fluorescent hydrogel kit for detecting the content of target biogenic amines (including histamine, cadaverine and putrescine), the fluorescent hydrogel kit has the advantages of being convenient to carry, rapid in detection and capable of achieving visual analysis, the detection method is sensitive and accurate, and the detection cost is low. The method can be applied to rapid and portable detection of biogenic amines in aquatic products, and is expected to play a role in the field of food quality control.

The application relates to the technical field of oral cavity equipment, and discloses a dental plaque quantitative analysis system, a method and a storage medium. The system comprises a dental plaque observation instrument and an upper computer. The dental plaque observation instrument is used for collecting a two-dimensional fluorescence image of teeth in an oral cavity area of a to-be-measured object. The upper computer is in communication connection with the dental plaque observation instrument. The upper computer is configured to: receive the two-dimensional fluorescence image of teeth sent by the dental plaque observation instrument; perform image segmentation and image depth estimation based on the two-dimensional fluorescence image of teeth, perform three-dimensional reconstruction, and obtain a three-dimensional tooth model containing a plaque area and a tooth area; then calculate the surface area of the plaque area and the surface area of the tooth area respectively, obtain an area ratio of the surface area of the plaque area to the surface area of the tooth area, and take the area ratio as a quantitative analysis result of dental plaque. The application can accurately calculate the area of the plaque area and realize high-precision quantitative detection of dental plaque.

The invention provides a magnetic medium performance detection device and method, and the device comprises a rotating assembly which is used for driving a magnetic medium to rotate; the magnetizing assembly is located on the outer side of the rotating circumference of the rotating assembly, and the magnetizing assembly is used for generating a plurality of magnetizing fields with different magnetic field intensities, so that when the magnetic medium rotates to the magnetizing range of the magnetizing assembly, the magnetizing assembly quantitatively magnetizes the magnetic medium; the detection assembly is located on the outer side of the rotating circumference of the rotating assembly, the detection assembly and the magnetization assembly are arranged at intervals, and when the magnetic medium rotates to the detection range of the detection assembly, the detection assembly quantitatively detects the performance of the magnetic medium and generates a detection signal; in each rotating period of the rotating assembly, the magnetic medium is magnetized by the magnetizing assembly and detected by the detecting assembly in sequence, so that one-time quantitative magnetization of the magnetizing assembly and one-time quantitative detection of the detecting assembly are completed. The problem that in the prior art, nondestructive quantitative detection cannot be carried out on the performance of the magnetic medium is solved.

The invention relates to the field of nanopores, in particular to a method for detecting a detection object in a sample based on nanopores. The method comprises the following steps: S101, mixing a sample with a detection system to form first annular nucleic acid with a gap; s102, adding ligase into the first annular nucleic acid with the gap, and incubating to obtain a first annular nucleic acid without a gap; s103, performing nanopore sequencing on the first circular nucleic acid product and the internal reference nucleic acid product; and S104, reading and comparing the first circular nucleic acid product and an internal reference nucleic acid product, and determining the existence and / or content of the detection substance in the sample based on a predetermined concentration-dependent curve of the detection substance. By utilizing a nanopore sequencing technology and introducing the designed circular nucleic acid, quantitative detection of miRNA and protein is realized, and the kit is suitable for clinical diagnosis.

The present invention relates to a kit and method for simultaneously detecting IL-6, IL-21 and T cell CD4 surface molecules in lymphoid tissue, belonging to the field of biomedicine; the kit comprises quantum dots for labeling IL-6 antibodies, quantum dots for labeling IL-21 antibodies, and quantum dots for labeling CD4 antibodies; the antibodies labeled by quantum dots specifically bind to antigens in lymphoid tissue respectively; CD4T cells have the ability to secrete IL-6 and IL-21, and the relationship between them and IL-6 and IL-21 reflects the tissue immune activity. The present invention provides a kit for simultaneously detecting IL-6, IL-21 and T cell surface molecules in lymphoid tissue, locating the spatial relationship between two cytokines and T cells, calculating and analyzing the corresponding fluorescence intensity, and evaluating the immune situation; the kit has the advantages of high fluorescence intensity, accurate detection results, wide spectrum selection, wide applicability, stable fluorescence structure, long storage time, high sensitivity, good specificity, etc.

The invention discloses a nitrofurantoin and adriamycin therapeutic drug concentration monitoring (TDM) electrochemical sensor and a preparation method and application thereof.The sensor is composed of a basic electrode and a composite material modified on the surface of the electrode, the composite material takes nitrogen-doped graded porous carbon as a substrate, and gold nanoflowers are generated on the surface in situ; the method comprises the following steps: preparing a CuNC material; uniformly coating the CuNC on the surface of an electrode; gold nanoflowers are electrically reduced on the surface; the electrochemical sensor is used for detecting nitrofurantoin and adriamycin; the method has the advantages of low detection limit and high sensitivity; the potential of medical and practical application is realized; the preparation and detection are simple and feasible.

The invention relates to the field of medical detection and discloses a colorectal cancer detection kit as well as a preparation method and a use method thereof. The colorectal cancer detection kit comprises a fluorescence labeling material and a lateral chromatography test strip, the fluorescent labeling material comprises fluorescent microspheres, an antibody corresponding to the colorectal cancer exosome marker and a primary antibody of an independent C line, wherein the antibody and the primary antibody are connected with the fluorescent microspheres; the lateral chromatography test strip comprises a composite pad, a detection membrane and a water absorption pad; one end of the detection film is lapped with the composite pad, and the other end is lapped with the water absorption pad; a T line close to the composite pad and a C line close to the water absorption pad are arranged on the detection film; the T line is coated with an exosome marker protein corresponding antibody; the C line is coated with a secondary antibody of an independent C line; the fluorescence labeling material and the lateral chromatography test strip are independently stored, or the fluorescence labeling material is attached to a composite pad of the lateral chromatography test strip. The colorectal cancer detection kit can realize detection of colorectal cancer marker protein, and further realizes detection of colorectal cancer.