22results about How to "Realize quantitative detection" patented technology

The utility model belongs to superconducting technical field discloses a kind of high-temperature superconducting tape wrapping and detection system, including pay-off device, cleaning device, wrapping device, pressing device, traction device, meter device, visual identification device and take-up device sequentially arranged along the output direction of superconducting tape, the high-temperature superconducting tape wrapping and detection system can effectively remove the dirt and metal chips and other pollutants on the surface of tape, pretreat before wrapping high-temperature superconducting tape, ensure insulation wrapping effect, and the stacking distance of insulating tape can be detected, the quantitative detection of insulation effect after superconducting tape wrapping is realized, which is beneficial to improve wrapping effect.

This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.

The invention discloses a preparation method and application of a high-stability hydrogen sulfide detection card, and the preparation method at least comprises the following steps: step I, dispersing dithizone in ethanol to obtain a dithizone solution; iI, dissolving lead acetate in water to obtain a lead acetate solution; iII, under the alkaline condition, mixing the dithizone solution obtained in the step I with the lead acetate solution obtained in the step II to obtain a dithizone-Pb complex; step IV, mixing the methanol solution containing tetrabutyl ammonium hydroxide, N, N-dimethylformamide, water and ethanol, and carrying out water bath reaction to obtain an alkaline silica gel solid carrier; v, dispersing the dithizone-Pb complex obtained in the step III into the alkaline silica gel solid carrier obtained in the step IV to obtain a dithizone-Pb complex solid carrier; and step VI, dispensing the dithizone-Pb complex solid carrier obtained in the step V on a thin film to obtain the hydrogen sulfide detection card. The detection card can be used for quantitative detection of hydrogen sulfide and drug sensitivity analysis of bacteria.

The invention provides a nitrite quantitative detection method based on surface enhanced Raman spectroscopy, relates to the technical field of surface enhanced Raman spectroscopy, and constructs a self-correcting SERS substrate through a three-phase liquid-liquid interface co-assembly technology. In the experiment process, a sample to be detected is mixed with a thiobarbituric acid solution, the mixture is adjusted to be acidic, NO2 <-> and thiobarbituric acid are subjected to an S-nitrosation reaction, and S-nitroso mercaptan is generated. And then, immersing the self-correcting SERS substrate into a reaction solution, so that the substrate adsorbs the generated S-nitroso mercaptan, and further, a Raman signal is enhanced. After soaking is completed, the substrate is taken out and dried, finally, a Raman signal is collected through a Raman spectrometer, and the concentration of NO2 <-> is accurately analyzed through the change of the signal intensity. According to the technical scheme, rapid and high-sensitivity detection of NO2 <-> is achieved, good selectivity and anti-interference capacity are achieved, and the method is widely suitable for detection requirements of multiple fields such as environment monitoring and food safety.

The application relates to the technical field of oral cavity equipment, and discloses a dental plaque quantitative analysis system, a method and a storage medium. The system comprises a dental plaque observation instrument and an upper computer. The dental plaque observation instrument is used for collecting a two-dimensional fluorescence image of teeth in an oral cavity area of a to-be-measured object. The upper computer is in communication connection with the dental plaque observation instrument. The upper computer is configured to: receive the two-dimensional fluorescence image of teeth sent by the dental plaque observation instrument; perform image segmentation and image depth estimation based on the two-dimensional fluorescence image of teeth, perform three-dimensional reconstruction, and obtain a three-dimensional tooth model containing a plaque area and a tooth area; then calculate the surface area of the plaque area and the surface area of the tooth area respectively, obtain an area ratio of the surface area of the plaque area to the surface area of the tooth area, and take the area ratio as a quantitative analysis result of dental plaque. The application can accurately calculate the area of the plaque area and realize high-precision quantitative detection of dental plaque.

The invention relates to the field of medical detection and discloses a colorectal cancer detection kit as well as a preparation method and a use method thereof. The colorectal cancer detection kit comprises a fluorescence labeling material and a lateral chromatography test strip, the fluorescent labeling material comprises fluorescent microspheres, an antibody corresponding to the colorectal cancer exosome marker and a primary antibody of an independent C line, wherein the antibody and the primary antibody are connected with the fluorescent microspheres; the lateral chromatography test strip comprises a composite pad, a detection membrane and a water absorption pad; one end of the detection film is lapped with the composite pad, and the other end is lapped with the water absorption pad; a T line close to the composite pad and a C line close to the water absorption pad are arranged on the detection film; the T line is coated with an exosome marker protein corresponding antibody; the C line is coated with a secondary antibody of an independent C line; the fluorescence labeling material and the lateral chromatography test strip are independently stored, or the fluorescence labeling material is attached to a composite pad of the lateral chromatography test strip. The colorectal cancer detection kit can realize detection of colorectal cancer marker protein, and further realizes detection of colorectal cancer.

This invention belongs to the field of electrochemiluminescence analysis and detection technology, and relates to an electrochemiluminescence sensor based on a defective titanium metal-organic framework enhanced with a pyrene-based self-assembled hydrogen-bonded organic framework, its preparation method, and its application. The process includes: drop-coating a PSA-HOF / D-Ti MOF dispersion onto a GCE electrode, drying it, adding a methamidophos aptamer, and allowing the reaction to complete at room temperature. The resulting aptamer-modified PSA-HOF / D-Ti MOF electrode is used as the working electrode, Ag / AgCl as the reference electrode, and a Pt electrode as the counter electrode. The electrode is then passed through a PBS phosphate buffer solution containing potassium persulfate, thus forming the PSA-HOF / D-Ti MOF system electrochemiluminescence sensor for the detection of methamidophos. The prepared PSA-HOF / D-Ti MOF system electrochemiluminescence sensor features simpler and more flexible operation, a wide detection range, a low detection limit, and low detection cost.

The application discloses a heat-resistant and wear-resistant elbow and straight pipe angle detection device and a use method thereof, and belongs to the technical field of pipe detection. The heat-resistant and wear-resistant elbow and straight pipe angle detection device comprises a bottom plate, and further comprises a detection seat, wherein the detection seat is vertically arranged on the bottom plate, and an arc-shaped groove is formed in the detection seat; a track frame is arranged on the outer side of the detection seat; a pushing assembly is arranged on one side of the detection seat; and a blanking channel is arranged on the lower side of the track frame; wherein the detection seat is provided with an adjusting assembly for adjusting the containing size of the track frame; the application integrates feeding, detection and blanking into an efficient single action through linkage pushing and an adjustable track; the deflection side plate and the displacement scale line are used to objectively quantify the elbow flatness error and replace subjective judgment; in combination with a measuring plate, the length of a straight pipe is synchronously detected, and efficient, accurate and adjustable continuous detection and automatic sorting are realized.

The invention discloses a cationic fluorescent probe with long wavelength emission as well as a preparation method and application of the cationic fluorescent probe. The fluorescent probe disclosed by the invention has the characteristic of emitting red fluorescence, the probe is combined with heparin through electrostatic interaction, and the fluorescence is quenched, so that the heparin is detected; furthermore, the protamine is specifically combined with the heparin, probe molecules are released, fluorescence is recovered, and the protamine is detected; the long-wavelength fluorescent probe provided by the invention has good selectivity and sensitivity on heparin and protamine, and can be applied to a serum dilution environment.

This invention relates to the field of health monitoring technology, specifically disclosing a method for health monitoring of photovoltaic modules and supports in mountainous photovoltaic power stations. The method involves connecting a capacitor and inductor in series between the positive and negative buses after disconnecting the inverter at night, injecting multiple sets of test voltages with superimposed dual frequencies and increasing amplitudes, and collecting the response signal on the negative terminal's ground sampling resistor. The amplitudes of the dual-frequency components and differential frequency intermodulation components are extracted through quadrature phase-locked loop (PLL). The ground parameters and differential frequency ground impedance modulus are solved by combining calibration coefficients and a parallel impedance model. Based on this, an equivalent nonlinear coefficient is calculated and fitted with the logarithm of the voltage amplitude. The nonlinear growth exponent is obtained by subtracting one from the slope to quantify the degree of micro-motion wear at the connection interface, achieving non-invasive online early warning.

This invention discloses a method for detecting the quality of power pole splices based on the back-transmission ratio of elastic wave signals, belonging to the technical field of power facility safety evaluation. The method includes: applying vibration to the pole and acquiring the elastic wave response signal; extracting the reflection amplitude A at the splice. b And the amplitude of the reflection from the top of the pole A t This invention introduces a distance attenuation coefficient κ to construct a back-transmission ratio ζ that eliminates the influence of excitation force. Based on the elastic wave multilayer medium propagation model, a nonlinear mapping relationship between the impedance ratio η of the joint and the rod and ζ is established, and η is obtained by solving the problem. The tightness of the joint is evaluated based on the value of η; the closer η is to 1, the tighter the connection. This invention eliminates the influence of impact force by introducing a back-transmission ratio ζ and compensates for the difference in propagation distance by using an attenuation coefficient κ, thus achieving quantitative and robust detection of the joint quality and overcoming the shortcomings of traditional methods that rely on the absolute amplitude of the signal and are easily affected by medium attenuation.

This invention discloses a metal nanoparticle reagent kit, its preparation method, and its application in the detection of eczema biomarkers. The metal nanoparticle reagent kit utilizes a chemical self-assembly method to deposit metal nanoparticles onto the surface of the kit's well plates. ADNA is modified onto the surface of the metal nanoparticles through Ag-S bonds. Subsequently, through a complementary pairing process between the ADNA and cDNA chains, a cDNA chain with a Cy3 terminus at one end is brought close to the metal nanoparticles to synthesize a nucleic acid aptamer sensor. The metal nanoparticle reagent kit can specifically recognize and rapidly capture eczema biomarkers using nucleic acid aptamers. Combined with surface-enhanced Raman spectroscopy, it achieves rapid, sensitive, and accurate qualitative and quantitative detection of eczema biomarkers. Simultaneously, the internally encapsulated internal standard molecule signal can dynamically correct the Raman signal in the metal nanoparticle reagent kit.

The present application relates to blood potassium detection test paper, preparation method and blood potassium detection device and working method belong to blood potassium detection technical field, solve the defects existing in current detection device, including the base, the middle layer, the blood cell filter layer and the hydrophobic layer which are arranged in turn, further including working electrode, reference electrode and thermistor, the working electrode, the reference electrode and the thermistor are installed on the base through the first groove of the base, and the first flexible pad layer is arranged between the working electrode, the reference electrode, the thermistor and the bottom groove surface of the first groove, the second flexible pad layer is arranged between the working electrode, the reference electrode, the thermistor and the middle layer, the cavity above the detection part of the working electrode, the reference electrode and the thermistor is arranged on the bottom surface of the middle layer, the middle layer and the hydrophobic layer are provided with blood flow channel, and the blood flow channel is communicated with the cavity, and the test paper of the application realizes quantitative and accurate detection of blood potassium concentration.

The invention discloses a metal organic framework immobilized enzyme based on three-enzyme cascade, a preparation method and application of the metal organic framework immobilized enzyme in alpha-Amy activity detection, and belongs to the technical field of biological engineering. According to the invention, alpha-Glu is taken as a core catalytic element, GOx and HRP are subjected to cascade immobilization, a three-enzyme cascade metal organic framework immobilized enzyme is constructed, and quantitative detection of alpha-Amy activity is realized. According to the method, a forward correlation model between the alpha-Amy activity and the TMB oxidation degree is constructed through the ratio of 2-methylimidazole to zinc acetate, optimization of synthesis time, the concentration ratio of alpha-Glu, GOx and HRP, the incubation temperature, the pH value and other key parameters of a reaction system, and a strategy is provided for accurate detection of the alpha-Amy activity. The detection system has the advantages of simplicity and convenience in operation, rapidness in detection, low detection limit, excellent stability, high specificity and the like, and has an important application prospect in accurate detection of alpha-Amy activity and clinical diagnosis of related diseases.

The invention provides a method for detecting copper metal particles in lithium carbonate powder. According to the detection method, a screen with a proper size is selected according to the particle size of the lithium carbonate, and the lithium carbonate can be effectively dispersed by utilizing the physical property that the lithium carbonate is slightly soluble in water and the wettability of an alcohol organic solvent, so that uniform and dispersed slurry is formed. According to the step, lithium carbonate can be rapidly filtered, copper metal particles are intercepted on the screen and do not react with metal, and rapid and effective separation is achieved. Furthermore, element composition can be accurately identified and the copper metal particles can be discriminated by performing energy spectrum analysis through quantitative analysis equipment, such as a flying-nano electron microscope, and then the size and the number of the copper metal particles can be accurately measured by utilizing an automatic size and number analysis program, so that quantitative detection is realized.

The application relates to the fields of material preparation and chemical analysis, in particular to a kind of preparation of MnO2 / AuOs composite material and method for detecting Pb 2+ Using MnO2 / AuOs. MnO2 / AuOs preparation includes MnO2 nanosheet, Au seed, AuOs, MnO2 / AuOs preparation. NaOH is added to manganese acetate solution, stirred, centrifuged, and the precipitate is dispersed in 2-mercaptoethanol aqueous solution to obtain MnO2 nanosheet. Gold seed is obtained by the reaction of tetrachloroauric acid and NaBH4, K2OsCl6 and ascorbic acid are added to the gold seed solution to obtain AuOs. AuOs is added to the MnO2 nanosheet solution, stirred to obtain MnO2 / AuOs composite material. Different concentrations of Pb 2+ are added to the MnO2 / AuOs composite material, acetic acid-sodium acetate buffer solution, 3,3',5,5'-tetramethylbenzidine solution and H2O2 solution are added, and Pb 2+ is detected by ultraviolet spectrophotometer. The MnO2 / AuOs composite material has peroxidase-like activity, Pb 2+ can form an alloy with gold in the material in the presence of 2-mercaptoethanol, thereby enhancing the peroxidase-like activity, so the composite nanomaterial can be used for detecting Pb 2+ .

This invention belongs to the field of chloride detection technology, specifically relating to a perovskite-based nanomaterial, its preparation method, and its application. The perovskite-based nanomaterial includes upconversion nanomaterials and Ag₂S quantum dots, with the upconversion nanomaterials and Ag₂S quantum dots linked by chemical bonds. The upconversion nanomaterials have a core-shell structure, with Yb 3+ / Er 3+ The perovskite-based nanomaterials form the core, which is then coated with a Cs₂NaYF₆ shell. The perovskite-based nanomaterials are added to the sample, and the emission intensity of the sample is measured in the 300–750 nm range under 980 nm laser excitation. The chloride concentration is calculated based on a standard curve. The perovskite-based nanomaterials prepared in this invention exhibit high detection accuracy in chloride detection, enabling quantitative detection of chloride at the micromolar level. Furthermore, the preparation method is simple and inexpensive.

The utility model relates to the field of product waterproof performance detection, in particular to a loudspeaker waterproof performance detection device. The device comprises a detection tool and a locking assembly, the detection tool is provided with an accommodating groove for placing a to-be-detected loudspeaker, a sealing cavity is formed between the bottom of the accommodating groove and the loudspeaker, an inflation channel communicated with the sealing cavity is arranged in the detection tool and is connected with an air tightness detector, and a pressure sensor electrically connected with the detector is arranged in the channel; the locking assembly is used for fixing the loudspeaker to be detected in the containing groove during detection. In addition, the utility model further relates to a bearing table of the groove corner of the containing groove, the specific composition of the locking assembly, the composition structure of the detection jig, a supporting frame and other related devices. The technical effects that the waterproof performance of the loudspeaker can be accurately detected, the detection stability and reliability are improved through cooperation of all the assemblies, and the loudspeakers of different specifications can be conveniently detected are achieved.

A norfloxacin sensor and its preparation and detection methods are disclosed, relating to the field of electrochemical sensor technology. The preparation method includes: S1 preparing a CTS / Ag-NiWO4 composite material; S2 preparing a multi-walled carbon nanotube solution; S3 polishing and activating the surface of a base electrode; S4 preparing a first solid film on the surface of the base electrode using the multi-walled carbon nanotube solution; and S5 preparing a second solid film on the surface of the first solid film using the CTS / Ag-NiWO4 composite material. This invention utilizes multi-walled carbon nanotubes, Ag-NiWO4, and chitosan to construct the sensor interface, enabling quantitative detection of norfloxacin concentration. It not only exhibits strong adsorption, catalytic, and conductivity capabilities, but also boasts a simple and easy-to-implement preparation and detection method, high sensitivity, and a low detection limit.

The invention discloses sgRNA, an editing plasmid, a recombinant plasmid, an AviTag engineered bacteriophage, a magnetic bacteriophage probe, and a construction method and application thereof. The invention further discloses an enrichment separation and quantitative detection method for the pseudomonas aeruginosa. The magnetic bacteriophage probe can rapidly and specifically enrich pseudomonas aeruginosa in complex matrixes such as serum, infectious microbes and interference components are removed through magnetic separation, and sensitive quantitative detection of target bacteria is achieved in combination with an ATP bioluminescence system. According to the invention, by optimizing the AviTag-linker configuration, the display and biotinylation efficiency of the AviTag tag on the surface of the bacteriophage is remarkably improved, on the premise of keeping the titer of the bacteriophage and the stability of a host spectrum, the capture capability of the magnetic bacteriophage probe is enhanced, and low detection limit, short detection time and good repeatability are realized.

The invention discloses a pipeline design and stress analysis bidirectional data integration verification method and system, and relates to the technical field of computer aided design. The method comprises the following steps: performing forward data conversion, extracting three-dimensional model data and generating a stress analysis model; an analysis result is returned, and a final model neutral file and multi-working-condition node displacement data are exported; dual-node matching and consistency verification are carried out, and a stable node corresponding relation is established through node identification and spatial distance; running state geometric reconstruction and preview verification: carrying out envelope calculation on multi-working-condition displacement to generate a running state model, and carrying out overlapping display on the running state model and the cold state model; automatic space interference detection and model updating are carried out, risk judgment is carried out through space index and bounding box pre-screening, and the three-dimensional model is updated after confirmation. According to the method, closed-loop verification between the design model and the analysis model is realized, the problems that data of a heterogeneous system are asynchronous and thermal deformation cannot be verified are solved, and the design consistency and convenience are improved.

The invention belongs to the technical field of light-emitting functional materials and temperature sensing, and particularly relates to a fluorescent temperature sensing material based on a 3d / 4f complex and a preparation method and application of the fluorescent temperature sensing material. The fluorescent temperature sensing material based on the 3d / 4f complex provided by the invention is prepared by taking a Schiff base ligand H2L, samarium nitrate hexahydrate and anhydrous zinc acetate as reaction raw materials through an evaporative crystallization method; the structural formula of the Schiff base ligand H2L is shown in the specification; the molecular structural formula of the fluorescent temperature sensing material based on the 3d / 4f complex is [ZnLSm (CH3OH) (NO3) 2]. The fluorescent temperature sensing material based on the 3d / 4f complex provided by the invention can keep stable in a wide temperature range, has a linear fluorescence intensity ratio, can realize quantitative detection or visual detection of temperature, is high in detection sensitivity, can provide a technical basis and a research and development direction for development of the temperature sensing material, and has a wide application prospect. And the method has excellent application value and prospect.