880results about How to "Realize quantitative detection" patented technology

The invention discloses a test paper reading and analyzing method and system based on a mobile terminal camera, which is used for calculating a concentration value of a to-be-detected substance in a detection sample and analyzing a test result. The method comprises the following steps: collecting, by the mobile terminal camera, a test paper image, analyzing and identifying the edge of a color developing area, and intercepting a required color development area; calculating a color value or a gray value of a test area (T line) and a control area (C line) in the color development area picture, and calculating the concentration ratio of the sample by utilizing the ratio of the color value or the gray value of the test area (T line) and the control area (C line); calibrating the sample concentration ratio in batches, substituting the sample concentration ratio into a standard curve of the test paper of the batch to perform the fitting operation, and calculating the real concentration value of the to-be-detected substance in the sample; looking for a matched detection result according to the real concentration value, and transmitting the detection result to the mobile terminal.

The invention discloses a method for preparing fluorescent microspheres immunochromatographic test paper strip and quantitative detection method. The invention takes the luminous nano-particles of dual-structure silicon dioxide compound organic dye as a marker, uses the immunochromatographic technology for preparing fluorescent microspheres immunochromatographic test paper strip, and then prepares a detection card which consists of a sample pad, a glass fiber membrane, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is fixedly provided with a detection line and a quality control line. In the detection process, the best excitation light souce of fluorescent microspheres is used for excitation; after the emitted fluorescence passes through a filter, a CCD scanning technology or fiber-optic technology is used for collecting, accumulating or multiplicating the emitted spectra which is then converted into a numerical signal; then the measured fluorescence intensity of the detection line is multiplied by a correction coefficient, and later the corrected fluorescence intensity is substituted in a standard curve which is preset in a fluorescence analyzer; and finally, the concentration of an object to be measured in the sample can be automatically calculated and obtained by the fluorescence analyzer. The invention has high sensitivity, accurate quantization and easy operation.

The invention discloses a laser scanning imaging nondestructive inspection method for hot continuous casting blank surface defects, comprising the following steps: 1) transversely irradiating a casting blank by a laser emitter to obtain a laser line; 2) scanning the laser line by utilizing an area array CCD image sensor; 3) extracting information representing the defect depths from the laser line so as to obtain a one-dimensional distance dot matrix formed by assembling the defect depths of the casting blank at the transverse position; and 4) mapping the one-dimensional distance dot matrix into gray level images with different gray levels; 5) splicing the gray level images at different transverse positions of a hot blank so as to obtain a whole gray level image; and 6) identifying the defects on the surface of the casting blank and reconstructing a casting blank surface tri-dimensional topographic image. The intention can effectively inhibit interference from a flaky scale on the high temperature casting blank surface which is not a defect but presents a defect state and water films so as to avoid the pseudo-defects from influencing detection precision, accurately identify defect target images and realize quantized detection of the defect depths, thereby realizing online detection of the defect states and depths of the surface of the casting blank under a high temperature state.

The invention discloses a quantitative detection method for specific nucleic acid fragments based on the CRISPR technology. The quantitative detection method comprises the following steps: acquiring atreated target nucleic acid sample, and adding the target nucleic acid sample and an aqueous-phase solution into a droplet generation device to generate reaction droplets, wherein the reaction droplets comprise at least one nucleic acid fragment to be tested; amplifying the nucleic acid fragment to be tested in the reaction droplets by using a recombinase polymerase amplification technique, and allowing target gene signals in the nucleic acid fragment to be tested to realize cascade amplification by using a gene editing technique; collecting a positive signal corresponding to a target gene, and converting the positive signal into image data; processing a fluorescence threshold in the image data by using the Poisson distribution principle so as to obtain the test corresponding to the number of the target gene, corresponding to the positive signal, in the nucleic acid fragment to be tested. Thus, rapid efficient quantitative detection of the concentration of the target gene is realizedbased on the CRISPR technology.

The invention discloses a uniform load surface curvature-based simply supported beam crack damage identification method. The method includes the following steps that: the modal parameters of a simplysupported beam before and after the simply supported beam is damaged are obtained through testing, flexibility matrixes are calculated on the basis of frequencies and vibration modes; a uniform load is applied to the simply supported beam, and the uniform load is multiplied by the difference of the flexibility matrixes which are obtained before and after the simply supported beam is damaged, so that a corresponding displacement difference is obtained; the curvature of the displacement difference is obtained, and a uniform load surface curvature difference is obtained; a relation between the position of a crack and the uniform load surface curvature difference of adjacent measurement points is built based on a linear interpolation theory, so that the accurate positioning of the position ofthe crack can be realized; a uniform load surface curvature-based structural stiffness damage degree identification method is built on the basis of relations between displacement curvatures and structural stiffness and bending moment; and a relation between the height of the crack and a uniform load surface curvature is built through a series equivalent linear stiffness model, so that the quantification of the height of the crack can be realized. With the method of the invention adopted, the position and height of the crack of the simply supported beam can be accurately positioned and quantified. The method is applied to the damage degree evaluation of the structure of a simply supported beam.

A method for nondestructively detecting residual stress on micro region includes utilizing XRD2 device to measure sample with residual stress to be tested to obtain XRD2 surface exploring atlas, then processing obtained atlas for obtaining a set of diffraction angle data varied in flowing Debye field angle variation at diffraction peak, obtaining relevant straight by using linear function to adapt data and obtaining relation of straight line slope to residual stress by adapting so as to calculate out residual stress of micro-region on measured sample.

The invention relates to qualitative and quantitative detection dual-purpose HCG test paper for colloidal gold immunochromatography assay. The test paper comprises a combined pad and an analysis membrane, wherein HCG monoclonal antibody complex labeled by colloidal gold is distributed on the combined pad; and a qualitative line containing HCG monoclonal antibodies or goat-anti-mouse antibodies, a quality control line and a quantitative line are sequentially distributed on the analysis membrane at fixed points according to a chromatographic flow direction. The invention has the advantages that the qualitative detection can be realized, the sensitivity reaches 25IU/L, the quantitative function is obtained, the situation of barbs caused by too high HCG content of the existing detection test paper is overcome, the linearity range can reach 100-15000IU/L through providing the corresponding quantitative detector for detection under the situation that samples to be detected are not diluted, the inconvenience and the error caused by sample pre-dilution can be effectively avoided, the detection precision is high, the accuracy is high and the requirements on clinical examination are satisfied.

The invention relates to an up-conversion fluorescent/magnetic nanomaterial-labeled immune-chromatographic test paper and a making method thereof. The immune-chromatographic test paper is constructed on the basis of optimizing the structure and all composition parts of a test strip through combining the fluorescent and magnetic characteristics of an up-conversion fluorescent/magnetic material with an immune-chromatographic technique. The immune-chromatographic test paper comprises a support material and an upper layer, and the upper layer comprises a sample pad, a binding and releasing pad, a cellulose membrane and a water absorbing material, wherein the cellulose membrane has a detection line and a quality control line, the detection line is coated with an antigen or antibody of an object to be measured, the control line is coated with a secondary antibody, and the binding and the releasing pad is loaded with an up-conversion fluorescent/magnetic nanomaterial-labeled antibody which can be bond to the object to be measured. The invention realizes the fluorescent quantitative and potentially magnetic quantitative detection of analytes in the fields of drug residual and the like based on the up-conversion fluorescent/magnetic nanomaterial-labeled immune-chromatographic technique, and can significantly improve the detection sensitivity of a traditional colloidal gold-labeled immune-chromatographic analysis method. The above test strip has the advantages of good specificity, high sensitivity, simple, intuitive and accurate detection, low cost, wide application range, and easy popularization and application.

The invention discloses a palladium nano particle dot matrix hydrogen sensor with controllable sensing parameters. The sensor comprises a pair of insulating flexible substrates (101) with metal electrodes (102), a Pd nano particle dot matrix (103) arranged between the electrodes, a deformation control device (105) for deformation of the flexible substrates, and a corresponding signal monitoring device (104) also arranged between the electrodes. According to the sensor, the work distance of a palladium nano particle dot matrix can be controlled to vary in multiple regions by regulation on strain, and thus, the sensing parameters, such as the sensitive pressure and the time, of the hydrogen sensor can be regulated effectively. The hydrogen sensor can regulate the hydrogen gas-sensitive property index of the Pd nano particle dot matrix in real time without changing the Pd nano particle deposit rate and the electrode structure. Further, the application range of the sensor is expanded and the use cost is lowered. The hydrogen sensor provided by the invention can be used in a plurality of fields related to hydrogen usage safety and needing quantitative detection on hydrogen in industrial and scientific research.

The invention discloses an electrochemical detection method for a DNA three-dimensional nanostructure probe, which sequentially comprises the following steps of: 1) synthesizing the DNA three-dimensional nanostructure probe by a self-assembly method, wherein the probe comprises an extended section of recognition sequence; 2) assembling the nanostructure probe onto the surface of a working electrode of an electrochemical device; 3) hybridizing target DNA and the probe on the surface of the working electrode; and 4) adding enzyme and a corresponding substrate for redox reaction and performing electrochemical detection by using the electrochemical device.

The invention discloses an immunochromatographic test strip, a detection method by using the immunochromatographic test strip, and application of the immunochromatographic test strip; the immunochromatographic test strip comprises a conjugate cushion, a testing line and a quality control line; the conjugate cushion is coated with Janus nano-particles marked by a detection antibody; the testing line is coated with a capture antibody or antigen; and the quality control line is coated with an antibody of the detection antibody. On the basis of the Janus nano-particles, double functions including visual read-out and fluorescent read-out of a target object can be realized; compared with a traditional colloidal gold immunochromatographic test strip, for the immunochromatographic test strip, the quantitative detection can be realized; and the relatively high detection sensitivity can be obtained.

The invention discloses a novel SERS substrate-based method for quantitatively testing pathogenic bacteria, and belongs to the technical field of biological detection. Silicon dioxide treated by an immunospectral marker is coated with gold nanoparticles for quantitative test of the pathogenic bacteria. Raman spectra signal monitoring data are more stable and reliable and can be greatly repeated. The method is good in test stability, high in sensitivity, high in specificity, the detection process is simple, fast, accurate and quantitative, and a wide detection interval and a low detection limit are obtained. The method is simple in operation, an SERS immune signal probe and a capture probe can be prepared in advance, the capture probe and the signal probe only need to be sequentially added to a to-be-detected sample for reaction during operation and the test is carried out at once through magnetic separation after the reaction is completed: the collection time is 30s, and then the concentration is immediately read from a standard curve, thereby achieving fast quantitatively testing. The method can meet the requirements of food safety and environment monitoring departments, and has wide practicability.

The invention provides a tumor exosome nanometer fluorescence sensor based on magnetic separation and DNA self-assembling. The sensor comprises a functionalized magnetic bead coupled with a GPC-1 antibody, a trigger chain, and three stem circular nucleic acid probes. According to the invention, quantitative detection of the GPC-1(+) exosome subgroup of the breast cancer is realized; and the detection specificity and sensitivity are high and the detection range is wide. The clinical application value of the method is evaluated by detecting the breast cancer GPC-1(+) exosome subgroup in the clinical plasma source specimen. The method having high universality not only can be applied to other biomarkers with specific aptamers screened out but also can be applied to detection of other exosome subgroups, proteins, cells and metal ions and the like.

The invention discloses a magnetic fluorescent microsphere immunochromatography quantitative detection method. In the method, respective excellent characteristics of magnetic nano particles and quantum dots are fully utilized, and an immunochromatography technology is combined to realize fluorescent quantitative detection on the basis of optimizing the structure and ingredients of a test strip. The method has a function of amplifying signals; and compared with the conventional colloidal gold immunochromatography method, the method has the advantages of high mark stability, low non-specificity, high sensitivity, wide linear range and accurate quantification. The invention provides a simple, accurate, specific and cheap detection tool for blood samples, urine samples, spittle, excrement and the like, so the method can be widely applied to the fields of medical technology, food safety, veterinary drug residues, environmental monitoring, drug detection and the like.

The invention provides a method and device for detecting hydrogen leakage of a vehicle-mounted hydrogen supply system and a fuel cell vehicle. The method comprises the steps: determining the current working condition of the fuel cell vehicle; determining a detection time period according to the current working condition; obtaining the hydrogen leakage quality of the vehicle-mounted hydrogen supplysystem in the detection time period; calculating the hydrogen leakage rate according to the hydrogen leakage quality and the detection time period; and when it is judged that the hydrogen leakage rate is larger than a preset value, determining that the hydrogen leakage occurs in the vehicle-mounted hydrogen supply system. According to the technical scheme provided by the invention, hydrogen leakage of the vehicle-mounted hydrogen supply system of the fuel cell vehicle can be quantitatively detected by calculating the hydrogen leakage rate, the accuracy and timeliness of hydrogen leakage detection are improved, and the problems of potential safety hazards and waste caused by hydrogen leakage are solved.

The invention discloses a fluorescence microballoon immunochromatography testing card for quantitatively testing HIV and preparation method. The testing card comprises A and B test paper, a sample cushion, a glass fibrous membrane, a nitrocellulose membrane and drinking paper, wherein, the nitrocellulose membrane is thereon fixed with a test line and a quality control line for realizing the simultaneous test of HIV antibody and HIV p24 antigen. The invention takes nucleocapsid dual-structural light-emitting nano-particles compounded by silicon dioxide and fluorescent substance as marks and adopts immunochromatographic technique to realize quantitative immunoassay of HIV. In the testing process, fluorescence microballoon excitation light source is adopted to carry out excitation, after the emitted fluorescence passes through an optical filter device, all emission spectra are collected, aggregated and multiplied by CCD scanning technique or optical fiber technique, and are then converted into numerical signals, the concentration of the substance to be measured is automatically calculated by using the built-in analysis software in a fluorescence analyzer. The invention has the advantages of high sensitivity, precise quota, fast detection, convenient operation, and economy and practicality.

The invention discloses an immunosensor based on hybrid chain reaction and single molecule counting. The immunosensor consists of a capturing antibody, a detection antibody, streptavidin, a biotinylated primer and a hairpin probe, wherein the capturing antibody is anti-TNF-alpha; the detection antibody is Bio-anti-TNF-alpha; a base sequence of the biotinylated primer (Bio-I) is 5'-AGT CTA GGA TTC GGC GTG GGT TAA TTT TTT TTT-biotin-3'; the hairpin probe consists of a hairpin probe H1 and a hairpin probe H2; a base sequence of the hairpin probe H1 is 5'-TTA ACC CAC GCC GAA TCC TAG ACT CAA AGT AGT CTA GGA TTC GGC GTG-3'; a base sequence of the hairpin probe H2 is 5'-AGT CTA GGA TTC GGC GTG GGT TAA CAC GCC GAA TCC TAG ACT ACT TTG-3'. The invention also provides a preparation method of the immunosensor and application of the immunosensor in detection of the TNF-alpha. The immunosensor disclosed by the invention has the advantages of high detection sensitivity, small sample use amount, no need of enzyme amplification and the like, and quantitative detection on low-concentration TNF-alpha can be realized.

The present invention relates to an electrochemical biosensor for detecting hydrogen peroxide, a preparation method and an application thereof. The preparation method comprises the steps of (1) synthesizing the silica-three-dimensional graphene composite material; (2) doping nitrogen and carbon into the synthesized silica-three-dimensional graphene composite material; (3) pretreating a glassy carbon electrode; (4) preparing the glassy carbon electrode modified by the nitrogen-carbon-doped silica-three-dimensional graphene composite material through the dispensing method; (5) putting the glassy carbon electrode into a chloroauric acid solution to prepare gold nanoparticle-nitrogen-carbon-doped silica-three-dimensional graphene glassy carbon electrode through chronoamperometry; (6) dipping the glassy carbon electrode into a double-sulfhydryl-modified GDNA solution and cultivating for 2-4 hours; (7) dipping the composite electrode into a hemin solution for 10-14 hours. The electrochemical biosensor is good in stability and reproducibility, high in specificity and sensitivity, fast and simple and convenient to operate. The electrochemical biosensor can be used for detecting the content of hydrogen peroxide in biological samples, cosmetics and the like.

The invention belongs to fire safety detecting equipment, relating to a smoke detector sensitivity detecting device based on the obscuration principle. The device provided by the invention is structurally characterized by comprising a testing loop, an aerosol generator, an air flow control fan and a processor, wherein the testing loop is composed of a testing barrel, a reflow tube, a testing room and a transparent smoke cup; and a smoke concentration detecting system based on the dimming principle is arranged in the testing room, and is composed of an alignment optical system, an absorption pool, a photoelectric detector and a galvanometer. When the device provided by the invention is used for detecting, aerosol smoke enters the absorption pool, the photoelectric detector receives the absorbed light intensity information after an incident light is absorbed and attenuated by the aerosol in the absorption pool, an obscuration coefficient can be obtained through the data processing, then the detected smoke concentration is obtained to be displayed on the liquid crystal display of the processor and compared with a standard concentration of a smoke detector alarm, the quantitative detection on the flexibility of the smoke detector can be realized depends on the alarming of an audible and visible alarm.

The invention relates to a potassium ion concentration detection method. According to the method, characteristics comprising that potassium ion regulation allows G-quadruplex DNA structure transformation to be formed and cyanine dye supermolecular aggregates identify the G-quadruplex DNA structure transformation are utilized, a sample to be detected is added to a mixed solution of G-quadruplex DNA and the cyanine dye, the absorbance value at 560-590nm, 500-540nm or 610-670nm or the fluorescence intensity value at 580-640nm is determined, and the corresponding potassium ion concentration value can be obtained through finding the value corresponding to the absorbance value or the fluorescence intensity value on a standard curve. The method has a high specificity, so the method is not affected by sodium ions in the sample; and reagent components is simple, and the reaction process is simple, so errors generated by operations can be effectively reduced, and the test accuracy is high. The method can be rapidly realized through a common ultraviolet-visible absorption detector, a spectrophotometer or a fluorescent spectrometer without special or extra instruments, so the detection cost is low, thereby the popularization and the application of the method in industries are convenient.

The invention discloses a magnetic nanoparticle biological probe, a preparation method and application thereof, in particular to a magnetic nanoparticle biological probe for detecting HBV preS1 antigen, a magnetic immunochromatography test paper strip, a preparation method and a use method thereof. According to the invention, magnetic nanoparticles with carboxyl modified surfaces are adopted as the carrier, the antibody of HBV preS1 is connected to the magnetic nanoparticles' surfaces through EDC and HBV to establish the preparation method of the magnetic nanoparticle biological probe for detecting the HBV preS1 antigen, and the biological probe is adopted as the marker and the antibody of the targeted marker is taken as the capture antibody to establish a sandwich type immunochromatography detection system. The magnetic nanoparticle biological probe has strong specificity, and has good signal sensitivity and stability. The magnetic immunochromatography test paper strip provided by the invetnion on the one hand can realize rapid qualitative detection of the HBV preS1 antigen through visual inspection, and on the other hand can realize quantitative detection of the HBV preS1 antigen through the quantitative magnetic signal of the captured probe.

The invention provides a preparation method of a fluorescence resonance system for rapid detection of ATP. The method comprises the following steps: step 1, preparing a Cy3 fluorescence-labeled ATP-aptamer; step 2, performing a coupling reaction for QDs with a complementary strand of the ATP-aptamer to obtain QDs molecular probes; and step 3, reacting the QDs molecular probes obtained in step 2 with the Cy3 fluorescence-labeled ATP-aptamer to obtain the fluorescence resonance system for detecting ATP after interaction of the ATP aptamer and the complementary strand. According to the invention, the method utilizes characteristics of specific binding of the ATP and the aptamer thereof as well as a resonance energy transfer technology, and thus realizes rapid detection of the ATP and real-time monitoring for changes of ATP concentration in mitochondria, thereby providing a novel method for detection and monitoring of the ATP in vitro and in vivo.

The invention relates to a method for detecting l,5-AG based on a persimmon tannin composite nano material. The method comprises the steps of the preparation of a composite nano material, the activation and modification of a silk-screen printing electrode, and the construction of a biological sensing interface. According to the method, H2O2 is generated by means of the signal amplification and excellent electron transfer effect of RGO/PT/Pt-Pd NPs, and the specific catalysis effect of PROD on the l,5-AG; the H2O2 is subjected to the catalytic decomposition of the RGO/PT/Pt-Pd NPs; generated electrons are transferred onto the surface of the electrode through the RGO/PT/Pt-Pd NP composite nano film; current response signals are measured through DPV; a working curve is drawn according to a relation between the concentration of the l,5-AG and the response current of a sensor; and the detection of the l,5-AG is realized.