109 results about "Incubation temperature" patented technology

The invention discloses a liquid detection device. A reagent card management and transmission mechanism (U) is used for orderly outputting and transporting reagent cards; a buffer liquid cup management and transmission mechanism (V) is used for orderly outputting and transporting buffer liquid cups to a rotary vibration plate (W); a mechanical arm (X) is used for carrying out pipetting, liquid extracting and liquid adding operations; an incubation bin (Y) mainly comprises a temperature control module for providing an appropriate incubation temperature for a charged reagent card, thereby making preparation for accurate detection of an optical detection zone (Z) in the next step; and the optical detection zone (Z) comprises an excitation light source module, a photoelectric conversion module, a control analysis module and a software system. Compared with the prior art, the liquid detection device disclosed by the invention is used for automatically detecting a to-be-detected liquid sample, the detection process is mechanically and automatically finished, and the operation in each step is realized without manpower, so that the efficiency is improved, the fussy manual operation is omitted, the automatic detection is realized, and the detection time is shortened.

The invention discloses a simple method for culturing haematococcus pluvialis to produce astaxanthin; the method comprises three culturing processes of narrow neck flask, plastic cask or Nylon bag, cement pit; each step passes through various physical and chemical environmental parameters, such as nitrogen and phosphorus contents, pH value, incubation temperature, illumination intensity, illumination time, exact adjusting and controlling measure of adding with halite or halogeno salt, H2O2 and FeCl2 and cell state, etc; each step has definite object, and reasonable control such that the haematococcus pluvialis has excellent vegetative growth, fast conversion of vegetative growth to non-vegetative growth, and accumulates much astaxanthin, thereby improving the efficiency and stability of culturing, converting and accumulating, and effectively preventing the pollution of mixed algae; because the invention is an open culturing and does not need closed photobioreactor, thereby greatly reducing the production cost and obviously improving the productivity, and open culturing haematococcus pluvialis to produce astaxanthin in large scale.

The invention discloses a method for screening an antithrombotic drug based on magnetic bead separation. According to the method, magnetic separation and chemical information collection (liquid chromatography-mass spectrometry) techniques are adopted; a target protein bonder is analyzed; the method is used for screening and evaluating the antithrombotic action of a drug. According to the method for screening the antithrombotic drug based on the magnetic bead separation, an optimal incubation temperature, an incubation time, a pH (potential of Hydrogen) value of a solution during incubation, ionic strength and a denaturizing cleaning solution are screened out through lots of experiments. A screening and evaluating method provided by the invention can be used for screening a large-scale natural product library or a combinatorial chemical library; in comparison with a conventional ultraviolet screening method, the screening efficiency can be obviously improved; a screening time is shortened; moreover, a synergistic effect between compounds can be discovered; the method for screening the antithrombotic drug based on the magnetic bead separation has the advantages of strong operability, quickness, high efficiency, accuracy, good repeatability and the like.

The invention relates to incubation and cultivation methods of agkistrodon acutus. An incubation method comprises the following steps of: egg selection and incubation, wherein the incubation comprises the following technical points of: a, controlling the incubation temperature to be 26-30 DEG C; b, controlling the relative incubation humidity to be 88-96 percent; c, incubating for about 23 days; and d, checking eggs timely, and processing mucid eggs: checking the eggs through lighting once a day to see whether mildew or fertilization occurs or not, and processing timely so as to avoid affecting other eggs. A cultivation method comprises the following steps of: feeding of the eggs, overwinter cultivation of the of agkistrodon acutus, and feeding and management of the of agkistrodon acutus. The incubation and cultivation methods of the agkistrodon acutus have high snakelet incubation survival rate, and fast agkistrodon acutus growth, and are suitable for large-scale artificial breeding.

The invention discloses a basal layer of a soft-shelled turtle incubator, and relates to the technical field of aquaculture. The basal layer comprises the incubator used for incubating soft-shelled turtles and is characterized in that a basal layer body is laid at the bottom of the interior of the incubator and comprises a cobblestone layer at the bottommost, a saw dust layer is laid on the cobblestone layer, an incubation sand layer is laid at the upper portion of the saw dust layer, a covering layer is laid at the upper portion of the incubation sand layer, soft-shelled turtle eggs are embedded in the incubation sand layer, the upper ends of the soft-shelled turtle eggs are flush with the upper end of the incubation sand layer, the covering layer is a river sand layer, and a constant-temperature heater is arranged between the saw dust layer and the incubation sand layer. According to the basal layer, due to the control over the incubation temperature, the humidity of the incubator and the humidity of incubation sand, the incubation rate of the male soft-shelled turtles is 100%, and the incubation cost is reduced; operation is easy and convenient, control is easy, the male rate is high, the incubated soft-shelled turtle survival rate is high, the temperature in the incubator is convenient to control, and the air permeability is good.

The invention discloses an artificial incubation and artificial brooding method for pelicans. The measures that the incubation temperature and the incubation humidity of pelican eggs and the temperature and the humidity of the growing environment of the young pelicans are controlled, and probiotics, digestive enzymes, vitamins, microelement and the like are added in a feed formula of the young pelicans are taken, so that the incubation rate of fertilized eggs is as high as 90%, the dead egg and developmental malformation rate is reduced to lower than 5%, the survival rate of the young pelicans in sixty days is higher than 80%, the nutritional requirement of the young pelicans is met through feeds, the feeds are easy to digest and absorb, and the survival rate of the newborn young pelicans in 0-20 days is as high as 85%.

The invention relates to a method for injecting to a single bird egg; the operation steps are as follows: the bird egg is positioned at a position with preset direction under incubation temperature; an opening is selected by a position on the egg shell which is to be injected to be disinfected by alcohol; a disinfected solid pin is used to form small openings in the same size on the opening of the egg shell and the outer shell membrane and the inner shell membrane below the opening; the bird egg is slightly rotated, so that the position in the egg to be injected aims to the small openings on the egg shell; a disinfected disposable injection needle is extended into the small openings; materials are injected into the egg by the disposable injection needle; the temperature of the egg is reduced in the environment 1 DEG C to 10 DEG C lower than the incubation temperature; the disposable injection needle is collected; the bird egg rotates for some angles within a longitudinal section, so that the crossing of the injection needle stays from the opening of the egg shell; melted paraffin is used to seal the opening; the paraffin is solidified under the incubation temperature; the bird egg is put to the original place to be incubated. The method has the advantages of low cost, fast operation, single-egg rejection, small simulation and damage, no pollution, fixed point and quantitative injection, and direct view.

The cultivation method of sea urchin tetraploid fingerlings has the steps that the urchin seedling is dried in shade for 0 to 40 minutes. The 0.5 to 2.0 ml 0.5 mol / L KCl solution is injected from the sea urchin membrana peristomialis for the spawing and spermiation. The urchine eggs and sperms are collected, and the hybrid fertilization is implemented. The developmental temperature of fertilized eggs is controlled between 17 to 18 DEG C. The fertilized eggs in concentration with a mix silk net are collected after 55 to 65 minutes of the fertilization, which are packed in a closed bag and disposed in a pressure vessel of a hydrostatic pressure equipment and the ambient temperature is identified between 17 to 18 DEG C. The pressure treatment is implemented after 60 to 70 minutes of the fertilization, in which the pressure is controlled between 70 to 80MPa and the processing time is controlled between 6 to 8 minutes. Afterwards the fertilized eggs are disposed inside a bin for hatching and stirred, and the incubation temperature is controlled between 16 to 18 DEG C. After floated, the embryos are selected with the proper aeration. The bait feeding is implemented during the larvae cultivation period. The water is exchanged 1 to 3 times everyday, and each time the quantity of water exchange is 1 / 3 to 2 / 3. The pond water is exchanged once every three days. The tetraploid fingerlings cultivated with the method can produce the triploid fingerlings, thus the time of processing and the time to the market are extended.

The invention discloses a method for hatching and breeding anser indicus. Aiming at the shortcomings that the hatching rate of the anser indicus by artificial hatching in the prior art is low and the method cannot be popularized in the actual breeding production, the invention provides a method for hatching and breeding the anser indicus of which the incubation hatching rate is over 90% under theconditions that the incubation temperature is 38 degrees centigrade + / - 0.1 degrees centigrade and the relative humidity is 56.0% + / - 2.0%. According to the method for artificial hatching and breeding the adult wild gooses in the invention, the adult wild gooses can be put in the breeding net to breed in open air, wherein the water area in the breeding net is 25% to 40% and the land area is 60% to 75%; the survival rate of the adult wild goose bred by the method reaches over 90%, the wild habits are good, and the ecological quality is high. The invention further provides a stereo breeding method of the anser indicus and plateau fish, which fully realizes the circulation economic model by matching a stereo breeding technology of breeding the wild gooses at the upstream and breeding the fish at the downstream with forage plant and methane utilization, and improves the overall earnings of the plateau breeding industry. The method has rational theory and simple method, and can easily control the artificial implementation conditions, so the method is suitable for popularizing the plateau area economic development.

The invention relates to a method for controlling saprolegniasis of odontobutis obscura germ cells in incubation period. The method comprises the steps: 1, firstly, cleaning and disinfecting an artificial fish spawning nest containing germ cells, and then placing the artificial fish spawning nest into a cleaned and disinfected incubation bucket; 2, adding the aerated tap water into the incubation bucket as incubation water for incubation, controlling the incubation temperature to be 20-28 DEG C in incubation, controlling the air oxygen flow rate to be 3.5-20L / min, replacing one third to half incubation water every 4 to 7 days; and entirely covering the incubation bucket with a covering object in days, and removing the covering object at night; and 3, breaking the membrane and emerging from the germ cell after 30-40 days. Before the germ cells are transferred into the incubation bucket, the germ cells and the incubation bucket are disinfected, and incubation water used in incubation process is disinfected; and the generation of saprolegniasis of odontobutis obscura germ cells under low-temperature condition can be effectively controlled by utilizing the biological characteristic that the odontobutis obscura germ cells has larger stickiness, and through controlling the output of an aerated pump, and proper incubation conditions are created as far as possible in the incubation process.

The invention discloses a sex control method for Chinese soft-shelled turtles, which includes: firstly, controlling the incubation temperature to be from 23 DEG C to 35 DEG C; and secondly, dropwise adding methyltestosterone to an animal pole of each fertilized egg. By means of temperature control and application of the methyltestosterone, the sex control method evidently increases a male proportion of Chinese soft-shelled turtles and meets market requirements.

The invention relates to a method of measuring transformation temperature of liposome, belonging to the analysis measuring field. The method is that while transformation temperature of some liposome is measured by DSC the relation curve of drug loading efficiency and incubation temperature; according to the incubation temperature corresponding to the highest peak of drug loading efficiency, actual transformation peak, namely accurate transformation temperature of the liposome can be determined in many heat absorption peaks in DSC map. The method can determine transformation temperature of liposome more accurately. If transformation point of some liposome is adjusted furthermore, only DSC is used to track the change of transformation peak and interference from other mixed peaks is avoided to measure the phase transformation of liposome quickly, conveniently and accurately.

The invention provides an artificial carassius auratus propagation method. The method is creatively improved in the aspects from the source of parent selection and parent fish rearing to the following aphrodisiac drug preference, incubation method design and the like, a complete technological method for artificially propagating carassius auratus is formed, and the propagation rate, the spawning rate and the incubation rate are remarkably increased. By the adoption of the preferred technical scheme, vitamin is added to fodder before parturition to promote gonad development of parent fish; the preferred temperature of water where the parent fish injected with aphrodisiac drug are located ranges from 21 DEG C to 22 DEG C, so that parent fish rutting and egg laying efficiency is further improved; the preferred incubation temperature ranges from 20 DEG C to 23 DEG C so that fertile eggs can be incubated. By the adoption of the technical scheme, a series of creative design is conducted on the technological conditions, the remarkable technical effect is achieved, the condition control angle and the condition control depth are remarkably improved or optimized compared with those in the prior art, and meanwhile the method is easy to implement, low in cost and remarkable in large-scale application prospect.

The invention discloses an intelligent poultry breeding disinfection incubator, which includes an air-drying box, a box body, a fan, a heater, a water tank, a camera, a partition board, a microprocessor, an ultraviolet light lamp, a hatching board, a groove and a partition bar. The box is provided with a guide plate, a heater is provided under the guide plate, an air-drying box is provided at the bottom of the side wall of the box body, a timer is provided on the outer wall of the box body, a radio transmitting device and a microprocessor are provided on the box body, The inner wall of the top of the box is equipped with ultraviolet light lamps. There are atomizing nozzles in the box. Divider, divider The Jiangnan Incubation Board is divided into multiple empty cabinets. There are grooves in the empty cabinets, and a camera is installed on one side of the partition board. The invention avoids the breeding of harmful bacteria from affecting the hatching rate and the health of chickens, ducks and other poultry, ensures suitable incubation humidity and temperature, has simple structure, is convenient to use, and is beneficial to popularization.

The present invention discloses a prevention and treatment method of saprolegniasis in an incubation period of hucho bleekeri fertilized eggs. The method comprises the following steps: from the second day of incubation of the fertilized eggs, the fertilized eggs are soaked using running water containing formaldehyde every day; soaking liquid enters form the bottom of an incubator and is discharged out from the top of the incubator, a flow rate is controlled, so that the fertilized eggs do not roll over due to the excessive flow rate, the operation is continuously conducted for 30 min each time, and in the soaking process, the operation is conducted in dark place without vibration. After 30 min, normal incubation water is used to conduct running water incubation, and in the stages from the development of the fertilized eggs to early incubation (at an average incubation temperature of 7.5 DEG C and an accumulated temperature reaching 127 DEG C-170 DEG C), formaldehyde disinfection is stopped. Artificial methods are used to remove dead eggs until the fertilized eggs come out from membranes. The method is used to conduct the disinfection of the hucho bleekeri fertilized eggs, the incidence of the saprolegniasis can be reduced by 80% or more, and the deformity rate can be reduced to be 3% or below after emergence by using the method,.

The invention discloses a partridge hatching egg incubating method at a constant temperature. The partridge hatching egg incubating method includes the steps of egg collection, storage, inspection, incubation and hatching. In the step of egg incubation, incubation temperature is controlled to be 37.7 DEG C in the 1st-21st days and 37.5 DEG C in the 22rd-25th days, and relative humidity is controlled to be 65%-70% in the 1st-21st days and 72%-75% in the 22rd-25th days; in the 1st-18th days, from 6 o'clock to 18 o'clock each day, eggs are turned one time every three hours, and from 18 o'clock to next 6 o'clock each day, the eggs are turned one time every four hours. The partridge hatching egg incubating method is beneficial to incubation of hatching eggs, adaptability of young birds is improved, survival rate of the young birds is increased, raising scale of partridges is guaranteed, and income of farmers is increased.

The invention provides a cationic polypeptide nano-aggregate and antibacterial application thereof, belonging to the field of preparation and application of antibacterial peptide. According to the invention, cationic polypeptide is dispersed in a solution; the cationic polypeptide nano-aggregate is formed through self-assembling by changing the concentration of the cationic peptide, the pH value of the solution, incubation temperature T and incubation time T; and then, the nano-aggregate is applied to sterilization. A solution with a cationic polypeptide concentration of 100 to 480 [mu]g / mL is subjected to inoculation on an oscillator at 35 to 38 DEG C (wherein a pH value is 6.5 to 8.5) for 30 to 60 min (360 rpm) for formation of the nano-aggregate through self-assembling. The nano-aggregate is mixed with a bacterial solution; and the nano-aggregate can kill a variety of bacteria efficiently and quickly, and bactericidal efficiency is up to 100% within 4 h.

A continuous temperature-gradient incubation apparatus designed to solve the problem of moving of liquid vapor generated on the high-temperature side toward the low-temperature side to thereby cause condensation.There is provided a multiwell incubation apparatus having a well-housing vessel made of a heat conducting material and, detachably housed therein, liquid-storing wells, the wells being arranged in transverse rows and longitudinal rows, the multiwell incubation apparatus being also provided with a liquid or gas flow channel or bath for supply of a gas saturated with vapor of the liquid, the multiwell incubation apparatus being capable of maintaining incubation temperatures differing between individual transverse rows of wells with a predetermined temperature difference, so as to realize a temperature series which forms a predetermined temperature difference profile along the longitudinal rows, characterized in that the multiwell incubation apparatus includes a heat source for realizing a temperature which is the lowest in the given temperature series, the heat source being disposed outside the well rows and along the transversely lined up wells close to a first side of the housing vessel; another heat source for realizing a temperature which is the highest in the given temperature series, the heat source being disposed outside the well rows and along a side opposite to the first side; and a separator provided for each transverse row of a certain temperature.

A preparation method of a titanium diboride composite material comprises: (1) mixing SiC powder, TiO2 powder, B4C powder, carbon powder, and TiB2 power with a resin binder; (2) putting the obtained mixture in a ball mill, and adding an organic solvent as a ball milling medium for ball milling; and (3) compacting the obtained product to obtain a blank material, raising the temperature to 1350+ / -20DEG C in a vacuum condition, performing thermal insulation, raising the temperature to 1850+ / -10 DEG C in an inert atmosphere, and performing thermal insulation sintering. TiB2 is introduced in the raw materials of the titanium diboride compact composite material, so that the problem TiB2 is generated due to probable halfway reaction of raw materials is overcome. The preparation method is simple,performance of the obtained composite material is excellent, and the composite material has bright prospects.

The invention belongs to the technical field of medicines, and discloses a preparation method of an oxymatrine active targeting lipidosome. The active targeting oxymatrine lipidosome is prepared by a pH gradient method. The optimal prescription for preparing the oxymatrine active targeting lipidosome is as follows: the ratio of medicine to lipidosome is 1:10, the ratio of phospholipid to cholesterol is 3:1 with additionally 10% mol of Lac-PE, the pH value of the outer aqueous phase is 6.8, the incubation temperature is 60 DEG C, and the average encapsulation efficiency is 55.48% under the optimal prescription. The oxymatrine active targeting lipidosome prepared by the method provided by the invention is relatively stable. In vitro drug release experiments of the active targeting lipidosome show that oxymatrine wrapped by the glycosylated lipidosome has a slow release effect. Through observation by a transmission electron microscope, the oxymatrine active targeting lipidosome prepared by the method provided by the invention is a single chamber lipidosome with the average grain size of 80nm, and meets the demand of lipidosome. In vitro dissolution experiments show that the oxymatrine active targeting lipidosome wrapped has a certain slow release feature.

The invention discloses a method for screening melanin formation resistant medicines on the basis of magnetic bead separation. Target protein conjugates are analyzed by the aid of magnetic separation and chemical information acquisition (liquid chromatography-mass spectrometry) technologies, and the method can be used for screening and evaluating melanin formation resisting effects of the medicines. The optimal incubation temperatures, the optimal incubation time, the optimal pH (potential of hydrogen) values of solution during incubation, the optimal ion strength and the optimal denaturation cleaning solution are screened by the aid of large quantities of experiments. Compared with the traditional methods for screening melanin formation resistant medicines, the method has the advantages that the screening efficiency can be obviously improved, and the screening time can be shortened; synergistic effects among compounds can be discovered, the method can be used for screening large-scale natural product libraries or combinatory chemical libraries, is suitable for screening the melanin formation resistant medicines (including chemically synthetic medicines and natural products) and discovering lead compounds at early stages, is speedy and accurate and is good in repeatability, and the like.

The invention discloses temperature-controllable incubation equipment. The temperature-controllable incubation equipment comprises an incubator body, a young output basket is arranged at the bottom end inside the incubator body, a temperature control box is fixedly installed at the portion, close to one side of the young output basket, at the bottom end inside the incubator body, air inlets are formed in the two sides of the incubator body, anti-dust devices are fixedly installed at the portions, close to the outer sides of the air inlets, of the two sides of the incubator body, and a containing groove is formed in the inner wall of the incubator body. The temperature control box is arranged and used for controlling the temperature in the incubator body; a temperature sensor is arranged and used for detecting the temperature inside the incubator body; through a refrigeration pipe, an effect of reducing the temperature is achieved; heating pipes are arranged and have the temperature increasing effect, the temperature inside the incubator body is kept constant accordingly, the suitable incubation temperature is provided for bird eggs, and embryonic development of the bird eggs is facilitated; by arranging the anti-dust devices, it is avoided that impurities such as dust enter the incubator body.

The sex of embryos in eggs is influenced or controlled through the application of light having selected wavelengths and heat in order to promote the development of embryos of a selected sex. An incubating device is provided having an interior cavity that can be sealed from an outside, and having a plurality of lighting elements disposed in the interior cavity. Eggs are disposed on trays, and pre-determined environmental conditions are applied to the interior cavity to promote hatching of the eggs. Concurrently with the application of the environmental conditions, the eggs are irradiated according to pre-determined lighting conditions having wavelengths substantially concentrated in selected ranges In some embodiments, the temperature of the incubator is kept above or below the optimal incubation temperature.

An eupolyphaga sinensis production and cultivation process comprises measures of storage of seed eggs, incubation of seed eggs, raising of larvas, management of adults and the like. The storage of seed eggs comprises putting the seed eggs in humid soil and controlling the temperature at 0-15 DEG C; the incubation of seed eggs comprises adding water into rice hull ash and fine earth which are mixed and proportioned in a ratio of 1: 1 to blend incubation soil; and putting the seed eggs in the incubation soil to be incubated, wherein the volume ratio of the seed eggs and the incubation soil is controlled at 2: 1 to 1: 1 and the incubation temperature is controlled at 28-30 DEG C; the raising of larvas comprises screening hatched larvas, putting in the larvas in an eupolyphaga sinensis pool, and adding raising soil to cover and raise the eupolyphaga sinensis once every evening till the eupolyphaga sinensis grows to adults; and the management of adults comprises selecting most male eupolyphaga sinensis to be dried in the sun after the larvas grow to adults, then adding materials to raise the eupolyphaga sinensis, screening a first batch of seed eggs three months later, screening the seed eggs once every month, and scalding female eupolyphaga sinensis by boiled water to be dried in the sun after the seed eggs are screened three times. According to the method for cultivating eupolyphaga sinensis, the eupolyphaga sinensis is high in yield and good in quality.

The invention discloses a heatable mushroom cultivation device, which comprises a cultivation box and a water tank arranged above the cultivation box. The heating chamber is connected with a blower device, the heating chamber is provided with a gas outlet communicating with the airflow chamber, and the inner wall of the airflow chamber is provided with a number of ventilation holes communicating with the cultivation chamber. The solution provided by the invention can control the temperature in the cultivation cavity, so that the temperature can always be adapted to the growth requirements of the mushrooms, making the survival and growth of the mushrooms easier, and increasing the output of the mushrooms.

The invention discloses an economical high-strength hot-rolled substrate galvanized sheet, C0.15%-0.65%, Si0.01%-3.50%, Mn1.10-5.00%, P≤0.50%, S≤0.030%, Al0 .02%~3.00%, V≤0.50%, Ti≤0.20%, Nb≤0.20%. The finishing temperature of hot rolling is controlled in the austenite zone, the cooling start temperature is controlled in the austenite zone or the two-phase zone of austenite and ferrite, and the M of the steel at the end of cooling is s Point and M f The temperature between the points, and then coiled; the hot-rolled sheet is immediately hot-dip galvanized after pickling, and the steel sheet is heated to 450-600°C in the continuous hot-dip galvanizing production line, and the holding temperature is 450-600°C, and then hot-dip galvanized.

The invention discloses a method for detecting the purity of a protein sample containing polyethylene glycol by using a capillary electrophoresis technology. The method comprises the following steps of (1) adding urea and related reagents into the protein sample for pretreatment; (2) incubating the sample at a proper temperature; (3) carrying out capillary electrophoresis analysis; and (4) calculating the purity of the protein sample according to the peak area ratio of different components, wherein the final concentration of urea and the incubation temperature are determined by a model. By adding urea and optimizing the incubation temperature, the problems of peak shape deformation, difficulty in accurate quantification and the like when the purity of a drug is measured by the traditionalcapillary electrophoresis are solved, and the accuracy and precision of the purity measurement are guaranteed.

The invention belongs to the technical field of breeding equipment, and relates to a cicada egg hatching device. It solves the technical problems such as the unreasonable design of the prior art. It includes an incubation box body, a water leakage hole, a water absorption layer and a heating mechanism. A ring-shaped egg branch positioning mechanism is arranged in the incubation box body, and an outer ring spray component located at the circumferential periphery of the egg branch positioning mechanism is arranged in the incubation box body. The ovum branch positioning mechanism is provided with an inner ring spray component in the circumferential direction, and the outer ring spray component and the inner ring spray component are fixedly connected, and the outer ring spray component is connected with a drive capable of driving the outer ring spray component and / or the inner ring spray component. A spray lift drive structure in which the shower assembly rises and falls in the vertical direction. The advantages are: adjustable incubation temperature, uniform temperature distribution in the incubator, convenient positioning of egg branches, uniform distribution of egg branches, uniform temperature and humidity around each egg branch, and improved hatching rate of cicada larvae on egg branches.