51 results about "Atp bioluminescence" patented technology

The invention relates to a method for detecting antibacterial performance of polyurethane synthetic leather. The method for detecting the antibacterial performance of the polyurethane synthetic leather comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation, excitation and bacterium suspension preparation are carried out; an OD value-viable bacterium content log value lgC standard curve is established and an inoculating bacterium solution C is calibrated; an lgC relative fluorescence intensity log value lgI standard curve is established; sample inoculating, cultivating, eluting and cultivating are carried out; recycled solution I measurement and variable bacterium content C and T calculation are carried out; an antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the antibacterial performance of the polyurethane synthetic leather is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the antibacterial performance of the synthetic leather represented by R or A. According to the method for detecting the antibacterial performance of the polyurethane synthetic leather, it is stipulated that control samples and antibacterial samples are recovered by elution after contact for 0h and cultivation for 24h and cycled, and a recycled solution I is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting antibacterial performance of the polyurethane synthetic leather, an advanced detection technology can be used for supporting product quality improvement.

The invention provides the tools needed for a dental patient's caries risk assessment. Included is a complete turnkey system for caries risk assessment and treatment, including an ATP test and a plurality of rinses and educational and diagnostic materials. The system for the detection and treatment of the bacterial infection that causes dental caries in a patient comprises a disposable ATP Bioluminescence sampler to swab areas of the patient's mouth, a bioluminescent light meter which measures the amount of ATP present in the patient's mouth, a rapid culture test for the detection of Mutans streptococci and lactobacilli, a diagnostic testing component that includes a caries risk assessment questionnaire, a treatment component that includes a short term therapeutic oral rinse of at least two components, and a long term maintenance rinse.

The invention relates to the technical field of air microorganism monitoring, and discloses a bioaerosol early warning device based on an ATP bioluminescence technology. The device comprises a reagent conveying module used for containing a reaction reagent and conveying the reaction reagent to a fluorescence reaction module; a sampling module used for collecting and conveying microorganisms in ambient air to the fluorescence reaction module; the fluorescence reaction module used for mixing the reaction reagent with the microorganisms and generating fluorescence; a monitoring and processing module used for detecting the fluorescence change, converting the fluorescence change into an electric signal, processing the electric signal to obtain the concentration of microorganisms, and sending an early warning signal when the concentration exceeds a preset value; and a waste liquid collection module used for collecting waste liquid after reaction of the fluorescence reaction module. The change of the concentration of microorganisms in air is obtained based on the ATP bioluminescence technology, an early warning signal is sent out when the concentration exceeds a preset value, the overall structure is simple, the function is reliable, the implementation is easy, and the working efficiency of the whole device can be improved.

The invention discloses a method for making firefly luciferase, comprising the following steps of: inserting the firefly luciferase gene into multiple cloning sites of a prokaryotic expression vector; obtaining a recombinant expression vector containing the firefly luciferase gene; introducing the recombinant expression vector into a colon bacillus cell to obtain a recombinant cell containing therecombinant expression vector; culturing the recombinant cell to obtain the firefly luciferase from the expression. The method for making the firefly luciferase has the advantages of simple process, short growth period, low cost, easy purification, high enzyme activity and stable performance and so on. The method can relieve a situation that the firefly luciferase mainly depends on import, and can promote the development of a fluorescent microorganism rapid detection system and the application of the firefly luciferase in medicine and environment science and so on, thereby providing excellentenzyme preparation for the rapid detection and applications in other aspects of ATP bioluminescence microorganism.