6 results about "Atp bioluminescence" patented technology

The application discloses a kind of magnetic capture material based on phage display, preparation method and application thereof, through the synergistic effect of specific capture and short-time proliferation, the detection limit of staphylococcus aureus is improved to 10 4 CFU / mL, reaches the detection limit of portable ATP bioluminescence instrument itself, ensures the reliability of detection result. At the same time, the whole detection process can be completed within 1 hour, only needs simple equipment such as magnetic separation frame and portable ATP detector, easy to operate, fast, suitable for food safety on-site detection, medical institution environment monitoring and various scenes. In addition, compared with traditional antibody, phage-derived binding polypeptide can be produced in large quantities by microbial fermentation, with lower cost and better batch consistency, and the directional display strategy based on T4 capsid avoids the activity loss caused by chemical coupling, further improves the stability of reagent, and has good application and promotion prospect.

The invention relates to the technical field of food safety, and particularly discloses a food safety detection method for food production, food safety can be evaluated from different angles through comprehensive judgment of two methods of real-time fluorescent quantitative PCR detection and ATP bioluminescence detection, the real-time fluorescent quantitative PCR detection has high sensitivity and specificity, and the real-time fluorescent quantitative PCR detection has high sensitivity and specificity. Low-concentration pathogens can be accurately detected, the ATP bioluminescence detection is used for evaluating the microbial contamination degree by detecting ATP generated by microbial metabolism in food, the two methods are combined, the miss detection risk possibly existing in a single detection method is avoided, when the production time is an odd number, the intermediate number of products in all products are sampled, and the detection accuracy is improved. And the intermediate products are located at relatively key positions in the production process, so that the reliability of safety evaluation of the whole batch of food by the detection result is improved, the detection efficiency can be improved and the detection period can be shortened while the detection accuracy is ensured.

The application provides a rapid detection method for total amount of microorganisms in traditional Chinese medicinal materials based on ATP bioluminescence, wherein the calibration set samples of the traditional Chinese medicinal materials to be detected are added into a buffer, pre-inoculation treatment is performed, the bacterial liquid, an additive and luciferase preparation are uniformly mixed, and then the relative light intensity is detected; the average relative light intensity value or the logarithmic value of the average relative light intensity value is used as the ordinate, the logarithmic value of the total amount of microorganisms is used as the abscissa, and a microorganism content distribution graph is drawn; the longitudinal qualified limit is delimited according to the microbial limit, the early warning limit is delimited according to the misjudgment rate, and partition is performed; whether the detection result is qualified is judged by the position of the average relative light intensity in the microorganism content distribution graph. The rapid detection method for total amount of microorganisms in traditional Chinese medicinal materials based on ATP bioluminescence has the advantages of fast testing speed, low detection cost, high sensitivity and the like, and is suitable for on-site detection of total amount of microorganisms of various traditional Chinese medicinal material varieties.

The invention discloses a cryptosporidium parvum sporozoites activity detection method and device and application, and belongs to the technical field of biological detection and drug screening. The method comprises the following steps: providing a to-be-detected sample containing dead or live cryptosporidium parvum sporozoites; diluting the to-be-detected sample in a multiple ratio gradient manner and / or performing freeze thawing treatment, and adding the to-be-detected sample into a detection container; an ATP bioluminescence detection reagent is added into the detection container for oscillation treatment, so that cryptosporidium parvum sporozoite is split to release ATP, and a reaction is carried out to generate a luminescence signal; collecting the light-emitting signal, and quantitatively evaluating the survival rate or activity of the cryptosporidium parvum sporozoite according to the intensity of the light-emitting signal. The method provided by the invention is based on the ATP bioluminescence principle, can directly, quickly and quantitatively evaluate the activity of the sporozoite stage in a short time, and can be used for high-throughput screening of large-scale compound drug libraries.

The invention discloses sgRNA, an editing plasmid, a recombinant plasmid, an AviTag engineered bacteriophage, a magnetic bacteriophage probe, and a construction method and application thereof. The invention further discloses an enrichment separation and quantitative detection method for the pseudomonas aeruginosa. The magnetic bacteriophage probe can rapidly and specifically enrich pseudomonas aeruginosa in complex matrixes such as serum, infectious microbes and interference components are removed through magnetic separation, and sensitive quantitative detection of target bacteria is achieved in combination with an ATP bioluminescence system. According to the invention, by optimizing the AviTag-linker configuration, the display and biotinylation efficiency of the AviTag tag on the surface of the bacteriophage is remarkably improved, on the premise of keeping the titer of the bacteriophage and the stability of a host spectrum, the capture capability of the magnetic bacteriophage probe is enhanced, and low detection limit, short detection time and good repeatability are realized.