2 results about "Atp bioluminescence" patented technology

The application discloses a kind of magnetic capture material based on phage display, preparation method and application thereof, through the synergistic effect of specific capture and short-time proliferation, the detection limit of staphylococcus aureus is improved to 10 4 CFU / mL, reaches the detection limit of portable ATP bioluminescence instrument itself, ensures the reliability of detection result. At the same time, the whole detection process can be completed within 1 hour, only needs simple equipment such as magnetic separation frame and portable ATP detector, easy to operate, fast, suitable for food safety on-site detection, medical institution environment monitoring and various scenes. In addition, compared with traditional antibody, phage-derived binding polypeptide can be produced in large quantities by microbial fermentation, with lower cost and better batch consistency, and the directional display strategy based on T4 capsid avoids the activity loss caused by chemical coupling, further improves the stability of reagent, and has good application and promotion prospect.