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Plate count. noun. : a determination of the degree of bacterial contamination of a sample (as of milk or semen) made by enumeration after a period of incubation of the colonies appearing in a plate that has been inoculated with a suitable dilution of the sample.
Electrochemical sensor for detecting total number of colonies in water body and preparation method and application thereof
The electrochemical sensor for detecting the total number of water bacteria colonies and its preparation method and application belong to the technical field of water detection. In order to solve the technical problems of long time consumption, inability to detect VBNC bacteria, lack of general target for detection, etc. in the existing water bacteria colony number detection method, the present application constructs an ultra-sensitive electrochemical sensor based on a triple signal amplification strategy by screening a general nucleic acid target fragment for water bacteria colony number detection, designing a corresponding hairpin probe and a hairpin structure capture probe. The sensor can realize rapid determination of the total number of water bacteria colonies, has good anti-interference, reproducibility and stability, can realize high-sensitivity detection of bacterial 16S rRNA gene, the linear range is 0.1 fM to 100 pM, the detection limit is as low as 1.0 aM; at the same time, it can realize actual detection of bacteria as low as 1 CFU / mL, and the detection result is highly consistent with the plate counting method, which is the "gold standard" for water bacteria colony number detection.
Owner:JILIN UNIVERSITY +1