471 results about "Lactobacillus acidipiscis" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu/g; lactobacillus acidophilus is not less than 1.0*10cfu/g; lactobacillus plantarum is not less than 1.0-10cfu/g; bacillus natto is not less than 1.0*10cfu/g; aspergillus niger is not less than 1.0*10cfu/g; and candida utili is not less than 1.0*10cfu/g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The invention discloses a method for preparing complete feed through probiotics asynchronous high-low-moisture fermentation and belongs to the technical field of microbial feed. The method comprises the following concrete steps that: firstly, protein feed is subjected to impurity removal and crushing, nutrition substances are added, mixed and stirred with clean water, and the moisture content is regulated to 60 to 70 percent; then, yeast, bacillus licheniformis and bacillus subtilis strain liquid is inoculated, high-moisture-content aerobic fermentation is carried out for 12 to 96 hours, and the fermentation temperature is 35 to 45 DEG C; then, energy feed is added, mixing and stirring are carried out, and the moisture content is regulated to 25 to 35 percent; next, lactobacillus lactis, enterococcus faecium, lactobacillus acidophilus, enterococcus faecalis and lactobacillus plantarum strain liquid is added, complex enzyme is added, the low-moisture-content anaerobic fermentation is carried out for 24 to 120 hours, and the fermentation temperature is 25 to 35 DEG C; and finally, the complete feed finished product is prepared through rolling tube drying, mixing and stirring, granulation and package. The method has the beneficial effect that the production efficiency and the feed nutrition value are improved.

The invention relates to a complex microorganism additive as well as a preparation method and application thereof, belonging to the biological material technical field of application of a microecological modulator in cultivating domestic animals. The preparation method thereof comprises the following steps: taking 30-60% of Saccharomyces cervisiae, 5-30% of Candida utilis, 5-30% of Rhodopseudom onas Palustris, 5-30% of Lactobacillus plantarum, 5-40% of Leatobacillus acidophilus and 5-25% of Bacillus subtilis; and sequentially carrying out primary bacteria strain culture, secondary fermentation and tertiary fermentation on the bacteria strains so that the bacterial number in fermentation liquor reaches 8-10*108/mL, thus obtaining the complex microorganism additive 808. Long-term on-site experiments prove that the product has reasonable formula, stable efficacy, disease prevention and resistance, improved quality and capability of promoting growth of animals and improving yield of animal products, survival rate and feed conversion rate.

The invention relates to the field of microbiology, and specifically relates to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is composed of a composite microbial fermentation broth and an embedding medium, wherein a volume ratio of the composite microbial fermentation broth to the embedding medium are in a range of 1:5 to 1:4. The compositemicrobial fermentation broth is compounded of a co-culture comprising Candida utilis, lactic streptococci, lactobacillus acidophilus and bacillus subtilis, and a single nutrient solution comprising Bradyrhizobium japonicum, Azospirillum lipoferum, Rhodopseudomonas palustris, Bacillus megaterium, Bacillus mucilaginosus and Azotobacter chrococcum. In the invention, composite microbes processed by embedding can be stored stably for more than 60 days and a count of the composite microbes is always more than 2 billion per gram, thus the composite microbial fertilizer comprising the composite microbes has a long effective time and can improve a planting capacity of soil.

The invention relates to a preparation method of a high-efficiency compound microorganism soybean meal leaven, belonging to the technical field of biological enzymolysis and fermentation. The preparation method comprises the following steps of: respectively carrying out optimal screening on lactobacillus acidophilus, lactobacillus lactis, saccharomyces cerevisiae, Candida utilis, bacillus subtilis and bacillus licheniformis; mixing and culturing by shaking a bottle; culturing by using a 10L fermentation tank; manufacturing a microorganism compound protective agent; spray drying in low temperature; preparing the soybean meal leaven and the like. The high-efficiency compound microorganism soybean meal leaven can efficiently inhibit the growth of mixed bacterium and eliminate or remarkably lower anti-nutritional factors and flatulent factors in soybean meal, enhance the protein utilization ratio and strengthen the immunity and has the excellent characteristics of acid resistance, salt resistance, temperature resistance and bile resistance. The invention has the advantages of simple preparation method, low cost, stable and reliable produced product quality and wide application range.

The invention discloses a probiotic composition for relieving anxiety or depression and an application thereof. The probiotic composition comprises prebiotics, a K-KLJ probiotic combination and functional substances. The K-KLJ probiotic composition contains at least one of lactobacillus plantarum, lactobacillus reuteri, lactobacillus casei, and lactobacillus helveticus, or also contains at least one of lactobacillus rhamnosus, lactobacillus acidophilus, bifidobacterium animalis and bifidobacterium longum. The probiotic composition of the present invention can significantly increase quantity ofbeneficial intestinal flora, improve intestinal discomfort, and reduce chronic inflammation, thereby reducing unhealthy emotion, and preventing emotional diseases such as depression and anxiety. Moreover, according to the invention, the combination of probiotic strains, the prebiotics, the probiotic strains and the functional substances have effect of significant synergistic enhancement.

The invention provides lactobacillus acidophilus freeze-drying composite protectant, and ingredients thereof includes: 9-11 parts by weight of dry buttermilk, 10-12 parts by weight of lactose, 0.3-0.6 part by weight of glycerol, 1.5-2.5 parts by weight of monosodium glutamate and 0.15-0.25 part by weight of Vitamin C. A freeze-drying protection method utilizing the lactobacillus acidophilus freeze-drying composite protectant includes steps of adding any type of lactobacillus acidophilus freeze-drying composite protectant into lactobacillus acidophilus liquor to generate protective liquor with the total weight of 130-180 parts by weight, and then freezing and drying. By utilizing the lactobacillus acidophilus freeze-drying composite protectant, higher freeze-driving survival rate of lactobacillus acidophilus is achieved with longer preservation, and the survival rate thereof is not easy to be reduced.

The invention discloses a preparation method of Se-enriched nutrient corn beverage, which is characterized by comprising the following steps of: using fresh sweet corn as raw material, conducting controlled hydrolyzing on fresh sweet corn steep liquor starch liquid by using amylase and maltogenic amylase, then using Lactobacillus acidophilus and Se-enriched beer yeast to carry two-stage fermentation on corn liquid, and obtaining a novel Se-enriched natural corn beverage product with rich health care functional factors and good taste.

The invention provides a method for preparing daily chemical microencapsulated probiotic. The method comprises the steps that: first, three probiotics which are lactobacillus acidophilus, lactobacillus plantarum and lactobacillus rhamnosus are fermented and centrifuged; such that wet cells are obtained; emulsifying, pre-freezing and freeze-drying are carried out; First embedding is carried out upon the obtained probiotic powder by using a crosslinked product of gelatin and TG enzyme; and second embedding is carried out by using a mixture of soluble starch, maltose and maltodextrin. With the method, the prepared microencapsulated probiotic has an embedding rate reaching 87%. The microencapsulated probiotic can be used in daily chemicals used for inhibiting acnes, relieving allergy symptoms, and lightening spots.

The invention discloses a micro-ecological composition for improving constipation by targeting intestinal flora and a preparation of the micro-ecological composition. The micro-ecological compositioncomprises probiotics, prebiotics and medicinal and edible traditional Chinese medicines, wherein the probiotics are selected from at least one of bifidobacterium animalis, bifidobacterium adolescentis, bifidobacterium longum, bifidobacterium breve, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus helveticus, lactobacillus plantarum, lactobacillus reuteri, lactobacillus paracasei and bacillus coagulans; the prebiotics are selected from at least one of stachyose, fructo-oligosaccharide, isomalto-oligosaccharide, galacto-oligosaccharide, inulin, resistant dextrin and xylooligosaccharide; and the medicinal and edible traditional Chinese medicines are selected from at least one of semen sesami nigri, semen cassiae, fructus cannabis, honey, semen lablab album, semen coicis, poria cocos and lotus leaves. The micro-ecological composition can effectively regulate intestinal tract micro-ecology in a targeted mode, and finally the purpose of improving constipation is achieved.

The invention relates to a method of cultivating biological flocs and an application thereof in breeding litopenaeus vannamei and relates to cultivation of biological flocs. The cultivated biological flocs comprise bacillus pumilus, lactobacillus acidophilus and pichia guilliermondii. The method comprises the following steps: preparing a bacillus pumilus inoculant, a lactobacillus acidophilus inoculant and pichia guilliermondii powder; adopting a way of splashing bacillus pumilus powder, saccharomycetes powder and a lactic acid bacteria inoculant for a water body per mu every day, and arranging an nano aerating tube in the lower layer around a cultivation pond to aerate to form the flocs; adjusting the C/N weight of a cultivating water body and controlling the C/N at (12-14): 1; and after the biological flocs are cultivated, splashing the bacillus pumilus inoculant, pichia guilliermondii powder and a lactobacillus acidophilus inoculant to the water body per mu every day. The cultivated biological flocs can be applied to breeding litopenaeus vannamei.

The invention aims to provide a production method for a compound microbial preparation for aquatic products. The production method comprises the following steps: respectively carrying out high-density liquid fermentation on strains of bacillus cereus, bacillus licheniformis, halophilic pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium to obtain ultra-concentrated bacterium powder; and then preparing according to a ratio to obtain the compound microbial preparation. The production process is simple in process, is applicable to most existing fermentation processes and equipment and is good for being popularized and applied in a large scale.

The invention provides a preparation method of lactobacillus acidophilus. The preparation method comprises the steps of: inoculating lactobacillus acidophilus of CGMCC No.2122; performing liquid state fermentation; performing solid fermentation; drying; and drying. The lactobacillus acidophilus prepared by the method is accompanied with other beneficial metabolites comprising lactein and organic acids such as L-lactic acid, n-butyric acid, acetic acid, formic acid and the like. The products are important energy sources of animal intestinal tract and can cooperate with probiotics to act on animals, so that the gastroenteric function of the animals can be obviously improved, the immunity is improved and growth is promoted. The products prepared by the method are used for preparing feed, so the digestion and absorption rate of the animals on the feed is increased.

The invention discloses a lactobacillus acidophilus composite freeze-drying protective agent and a preparation method and a usage method thereof. The protective agent comprises lactose, galactose, trehalose, Na2HPO4, sodium ascorbate and MgSO4. Compared with the prior art, the protective agent don not contain glycerol, so that the livability (above 85%) and the stability of freeze drying preservation of the lactobacillus acidophilus, the storage period (the period is 1 year, mycelia powder viable count is more than 1011cfu/g) of the lactobacillus acidophilus is prolonged, and no milk composition is included, and the agent can be used widely in non-dairy products. The preparation method and the usage method are simple, easy to operate and low in cost.

The invention provides a preparation method of compound lactobacillus-fermented sauerkraut, comprising the following steps of: firstly preparing three strains to produce leavening agents, i.e., starterleuconostoc pseudomesenteroides, lactobacillus plantarum and lactobacillus acidophilus, wherein the viable count of the leavening agent produced by each strain is greater than or equal to 109 viable count/ml; drying the leavening agents produced by three strains in a freezing way; then according to freeze-dried strain powder, weighing 3-5 parts of starterleuconostoc pseudomesenteroides A, 2-3 parts of lactobacillus plantarum B and 1-2 parts of lactobacillus acidophilus C, sufficiently mixing to prepare leavening agent freeze-dried powder; subsequently, selecting and washing Chinese cabbage, adding the leavening agent freeze-dried powder of which the weight is 0.012% as heavy as the weight of the Chinese cabbage, carrying out heat preservation and standing sealing and fermenting for 120-125hours at 30 DEG C; and finally, draining moisture, cutting into shreds, and carrying out vacuum packing to prepare a finished product.

The invention belongs to the technical field of biology and particularly relates to probiotics traditional Chinese medicine preparation for relieving yin-deficiency type constipation of elder people and a preparing method and application of the probiotics traditional Chinese medicine preparation. The probiotics traditional Chinese medicine preparation comprises traditional Chinese medicine and probiotics, wherein the traditional Chinese medicine comprises, by weight, 30-70 parts of rhizoma polygonati, 1-15 parts of radix polygonati officinalis and 10-25 parts of semen cassia; the probiotics are one or more of lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus and bifidobacterium adolescentis, and 108-109CFU of probiotics is mixed into each gram of the probiotics traditional Chinese medicine preparation. The preparing method of the probiotics traditional Chinese medicine preparation is simple to operate and easy in industrial production. The probiotics traditional Chinese medicine preparation is capable of relieving the yin-deficiency type constipation of elder people and capable of adjusting intestinal flora balance and increasing immunity at the same time, safe, long in storage life, and capable of being made into powder, tablets, capsules and the like.

The invention relates to a preparation method of a freeze-dried powder preparation of Lactobacillus acidophilus, Lactobacillus paracasei or lactobacillus rhamnosus, and provides a culture medium used in the freeze-drying process of Lactobacillus acidophilus, Lactobacillus paracasei or lactobacillus rhamnosus products. The inventor combines two critical factors influencing bacterial fermentation together, and creatively carries out fermentation in a high-density fermentation process by means of combination of two-sectional pH and three-sectional temperature variation, so that the relatively single form of the maximum cell biomass and the highest fermentation activity is improved remarkably, meanwhile, the obtained freeze-dried preparation is high in viable count and excellent in stability. The invention also provides an excellent freeze-drying protective additive.

The invention discloses a protein feed raw material prepared from Asian carps and is used for animal breeding and a preparation method for the protein feed raw material. The preparation method comprises the steps of performing smashing pretreatment on the Asian carps, mixing the smashed Asian carps with a microorganism preparation, and then performing fermentation so as to obtain the high-quality protein feed raw material with balanced protein and amino acid. The protein feed raw material can be widely applied to livestock breeding and animal aquaculture. Strains of a microorganism leavening agent comprise at least three of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, lactobacillus acidophilus and pediococcus pentosaceus. A method which is low in energy consumption and low in cost and does not have any waste emission is supplied to deep processing of the Asian carps, so that the ecological problem caused by the Asian carps in America can be effectively controlled, and protein resources of the Asian carps are fully used; the prepared protein feed raw material can guarantee no loss of nutrition of the raw material, and polypeptides, cellulose, oligopeptide, oligopeptide mixtures, trace elements and the like can be generated; the optimal nutrition is supplied to animal breeding.

The invention provides a compound microorganism preparation. The compound microorganism preparation is composed of Bacillus cereus, bacillus licheniformis, halophilic pseudomonades, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium. The viable count of the compound microorganism preparation is as high as 8*1011CFU/g, and the compound microorganism preparation can be widely applied to the aquaculture water areas of fresh water and seawater. Ammonia nitrogen and nitrite in the water can be effectively degraded and water blooms can be restrained under the synergistic effect of the bacterial strains, therefore, the aquatic water can be purified, growing environment for breeding animals can be improved, and productivity can be improved.

The invention relates to an escherichia coli genetically engineered bacterium and a method for efficiently producing L-tryptophan by utilizing the escherichia coli genetically engineered bacterium. The genetically engineered bacterium is obtained by correspondingly transforming and combining L-tryptophan synthesis-related genes in escherichia coli through a metabolic engineering method (includinggene integration, point mutation and promoter substitution) and introducing a glutamine synthetase coding gene glnA from lactobacillus acidophilus on genome of the escherichia coli. The strain is usedfor carrying out shake-flask fermentation; 10 to 12g/L of L-tryptophan can be accumulated within 24 hours, reaches the maximum value reported in China and is improved by 15.1 percent compared with that of an existing L-tryptophan industrial strain carrying plasmid, which shows that the strain has good potential for industrially producing the L-tryptophan.

The invention relates to a lactobacillus acidophilus preparation and application thereof, in particular to a micro-ecological preparation using lactobacillus acidophilus as a main active ingredient and application of the micro-ecological preparation in treatment of irritability and other related diseases and belongs to the field of biological medicines. The lactobacillus acidophilus is lactobacillus acidophilus DH213, and the preservation number is CGMCC No.11228.

The invention relates to lactobacillus acidophilus with high gastrointestinal tract stress resistance, wherein the preservation number of the lactobacillus acidophilus is CGMCC No.10436. The screened lactobacillus acidophilus has high gastrointestinal tract stress resistance, and can be applied to fields of food industry, medicine and the like, and thus a theoretical basis is laid for the further research on the gastrointestinal tract stress resisting mechanism of the lactobacillus acidophilus.