91 results about "Intestinal contents" patented technology

The invention provides an intestinal postoperative fistula-proof device which comprises a fistula-proof membrane assembly and a degradable near-end ring mechanism, and the fistula-proof membrane assembly comprises a membrane cylinder and a first storage cavity; wherein the first storage cavity is formed in one end of the thin film cylinder, the first storage cavity is formed by turning over one end of the thin film cylinder, a degradation type near-end ring mechanism is arranged in the first storage cavity, and the end, away from the first storage cavity, of the thin film cylinder is fixedly connected with a far-end supporting ring; the degradable near-end ring mechanism is matched with the film cylinder to be fixed to an intestinal canal in a sewing mode, then the far-end supporting ring is moved to drive the film cylinder to completely cover an anastomotic stoma, the far-end supporting ring is used for supporting the film cylinder so that intestinal contents can be isolated from making contact with the anastomotic stoma, the anastomotic stoma is fully protected, and the anastomotic stoma is prevented from being damaged. And the whole anti-fistula device is a smooth channel, so that the resistance of intestinal contents is reduced, and the risk that the intestinal contents fall off in advance is effectively reduced.

The invention discloses a non-invasive intestinal flora fixed-point sampling and drug release device, and belongs to the technical field of micro-ecological diagnosis and treatment. The device comprises a shell, an internal storage bin, a medicine loading cavity and a channel switching mechanism. The shell adopts a capsule-shaped design, and an enteric coating is arranged on the surface of the shell, so that the function is started after the shell reaches a specified position of an intestinal tract. Through the internal ingenious mechanism linkage design, the device can firstly complete non-invasive fixed-point sampling of intestinal contents, and after the sampling action is completed, a drug release mechanism is automatically triggered, so that accurate drug delivery of the same part is realized. Cooperative operation of sampling and drug release in time sequence and space is achieved, and the problems of pain, disjunction of the diagnosis and treatment process, poor drug targeting and the like caused by traditional invasive operation are effectively solved. The device is flexible in structural design, has the advantages of being noninvasive, efficient, accurate in time sequence control and the like, and is suitable for integrated application of diagnosis and treatment of various digestive tract diseases such as inflammatory bowel disease management, micro-ecological regulation and the like.

The invention provides a preparation method of a Witzia ciliaris IOB502 microbial inoculum and an application of the Witzia ciliaris IOB502 microbial inoculum in the aspect of recovering intestinal motility, the Witzia ciliaris IOB502 microbial inoculum is prepared by adopting a method of carrying out low-oxygen stress staged fermentation on chickpeas by adopting the Witzia ciliaris IOB502, and the generation of vitamins B1 and B2 can be promoted. The microbial inoculum does not affect physiological development of important organs such as liver, spleen and small intestine of mice, can improve defecation symptoms of the mice with constipation, increase defecation times and water content of excrement, improve intestinal movement of the mice with constipation, promote smooth passage of intestinal contents, effectively improve colon injury of the mice with constipation, and improve the constipation rate of the mice with constipation. Comprising mucous membrane layer thinning and mucus secretion reduction.

The invention belongs to the field of microorganisms, and relates to lactobacillus paracasei. In particular discloses a lactobacillus paracasei strain HNGD-AL1, the taxonomic name is Lactobacillus paracasei, the lactobacillus paracasei strain HNGD-AL1 is preserved in China Center for Type Culture Collection on February 27, 2025, the preservation number is CCTCC M 2025303, and the preservation address is No. 299 on eight road in Wuchang District, Wuhan, Hubei. The method for degrading the sulfur-containing amino acid by adopting the lactobacillus paracasei is found for the first time, the effect of degrading 53.39% of the sulfur-containing amino acid can be achieved by culturing the probiotics in a fermentation culture medium for 24 hours, and the probiotics can be colonized in intestinal tracts and play a role in degrading the sulfur-containing amino acid in intestinal contents; the content of metabolite hydrogen sulfide of sulfur-containing amino acid in the intestinal tract is reduced by 60.92%, intestinal tract tissue damage, permeability and inflammatory response are reduced, and the purpose of improving ulcerative colitis is achieved. The preparation method has a wide application prospect in the aspects of preparation of low-sulfur-content amino acid foods and probiotic preparations for improving colitis.

The invention discloses lactobacillus paracasei CFCFC018, a microbial agent and application of the lactobacillus paracasei CFCFC018, and belongs to the technical field of microorganisms. The lactobacillus paracasei CFCFC018 is separated from intestinal contents of rural cats in half-farming and half-pastoral areas in Inner Mongolia, does not need genetic engineering modification, can highly express amino acids and neurotransmitters, can remarkably relieve mammary gland alveolar structure damage in mammary gland tissues of rats suffering from mastitis induced by staphylococcus aureus, and has the advantages of being high in safety and good in stability. The thickening of a mammary gland interacellular matrix layer is avoided, the infiltration number of neutrophils is reduced, the damage degree of mammary gland tissues is reduced, and a certain protection effect on the mammary gland tissues is achieved. The research also shows that the strain can reduce the abundance of harmful bacteria in excrement, improve intestinal inflammation and relieve edema of intestinal submucosa, which provides a new thought for preventing and treating mastitis by regulating intestinal microbiota.

The invention relates to the field of medical apparatus and instruments in rectum surgery, and particularly provides a novel multifunctional intestinal bypass device for rectum surgery, which comprises a hollow sleeve, a film sleeve, a decompression air bag sleeved outside the hollow sleeve, and an inflation channel, wherein after being inflated, the decompression air bag protrudes in a corrugated or threaded mode in the axial direction of the hollow sleeve, the protruding part makes contact with the intestinal wall to provide friction force, and a decompression gap is formed between the sunken part and the intestinal wall; according to the invention, intestinal contents are discharged from the hollow sleeve and the film sleeve, so that excrement or intestinal juice is prevented from being in contact with an anastomotic stoma, the infection risk of the anastomotic stoma is reduced, and the healing rate of the anastomotic stoma is accelerated; the thin film sleeve is soft and deformable, so that discomfort such as anus falling expansion, pain and tenesmus of a patient can be effectively reduced, and the comfort of the patient is improved; the decompression air bag is expanded through the inflation channel, the hollow sleeve is positioned, meanwhile, blood supply of the intestinal wall is protected, and the reliable effect of replacing preventive fistulization is achieved.

The invention discloses an intestinal tract flow field modeling method and device based on bidirectional fluid-solid coupling. The method comprises the following steps: S1, collecting intestinal tract multi-modal data; wherein the multi-modal data comprises geometric parameters, motion characteristics, content statistics and wall stress strain data of the intestinal tract; s2, performing three-dimensional reconstruction on a human body real intestinal tract geometric model; s3, performing preprocessing of format conversion and simplified modification on the established intestinal tract geometric model; s4, dividing the preprocessed model into a model fluid domain and a solid domain, and establishing a bidirectional fluid-solid coupling model architecture; s5, performing fluid domain simulation setting: simulating multiphase flow contents, and setting a fluid simulation model; s6, performing solid domain simulation setting: setting intestinal wall material parameters, and simulating intestinal movement; and S7, bidirectional fluid-solid coupling is realized, and an intestinal tract flow field is simulated. Bidirectional interaction between an intestinal tract wall solid domain and an intestinal tract content fluid domain is considered, and more accurate intestinal tract flow field simulation is achieved.

The invention belongs to the technical field of fermented feeds, and relates to a method for preparing a fermented feed by using bovine intestinal lactobacillus, and the method specifically comprises the following steps: step 1, mixing intestinal contents with pre-sterilized normal saline, filtering, and adding amino acids; 2, adjusting the pH value of the intestinal tract solution, carrying out anaerobic culture, and then mixing with an adsorption carrier to obtain a solidified microbial inoculum; step 3, mixing the feed, the curing microbial inoculum and the filtrate in the step 2, and fermenting according to three stages; 4, after fermentation is completed, drying is conducted to obtain the fermented feed; through the synergistic effect of various technologies such as specific strain screening, a multi-stage dynamic regulation and control process and compound bacteriostasis, the core problems of poor strain adaptability, low fermentation efficiency, easiness in pollution, high storage cost and the like in the existing fermented feed are systematically solved.

The present invention belongs to the technical field of fermented feed and relates to a method for preparing fermented feed by using bovine intestinal lactobacilli. The method specifically comprises the following steps: step 1, mixing intestinal contents with pre-sterilized physiological saline, filtering and adding amino acids; step 2, adjusting the pH of the intestinal solution, anaerobic culturing, and then mixing with an adsorption carrier to obtain a solidified bacterial agent; step 3, mixing the feed, the solidified bacterial agent and the filtrate of step 2, and fermenting in three stages; step 4, drying after the fermentation is completed to obtain the fermented feed. The present invention systematically solves the core problems of existing fermented feed, such as poor strain adaptability, low fermentation efficiency, easy contamination and high storage cost, by synergistically acting through multiple technologies such as specific strain screening, multi-stage dynamic control process and composite antibacterial.

The utility model discloses a neonatal closed enemator, and particularly relates to the technical field of medical auxiliary instruments, the neonatal closed enemator comprises an enema mechanism, the upper side of the rear end of the enema mechanism is fixedly connected with a flushing mechanism, and the lower side of the rear end of the enema mechanism is fixedly connected with a waste liquid collecting mechanism; the upper end of the inner surface of the flushing mechanism is fixedly connected with a temperature monitoring mechanism, and the rear ends of the flushing mechanism and the waste liquid collecting mechanism are jointly connected with a supporting mechanism in a clamped mode. When the neonatal closed enemator is used, a flushing bag is used for storing flushing fluid, the flushing fluid can be injected into a system through a flushing pipe and an adjusting valve seat, and a drainage anal tube is used for conveying the flushing fluid to the intestinal tract of the neonatal from the flushing bag and the flushing pipe. Meanwhile, intestinal contents are drained to the collecting tube and the waste liquid bag, liquid and excrement discharged from the drainage anal tube are collected and stored, and the temperature monitor monitors the temperature of enema liquid in the flushing bag through the monitoring probe.

The invention belongs to the technical field of soil remediation, and relates to a method for remediating norfloxacin contaminated soil by using earthworm intestinal contents, which comprises the following steps: inducing earthworms to empty the intestinal contents on quartz sand by illumination, extracting by using a phosphate buffer solution, activating nutrient solution and treating by using a modifier to obtain high-activity modified earthworm intestinal contents, and granulating with activated carbon, organic acid and mineral materials according to a specific ratio, and embedding and curing to prepare the repairing agent. After the remediation agent is applied to the contaminated soil, efficient decomposition of norfloxacin and synchronous restoration of the ecological function of the soil are achieved through the degradation effect of earthworm intestinal microorganisms, the adsorption effect of activated carbon, the complex reaction of organic acid and physical regulation and control of mineral materials. The method creatively utilizes natural microbial communities in the intestinal tract of the earthworm, has the advantages of thorough degradation, environmental compatibility, low cost, simplicity and convenience in operation and the like, and effectively overcomes the problems of chemical residues, poor microbial adaptability, long repair period and the like in a traditional repair method.

The invention discloses an intestinal microneedle anastomosis stent with an antibacterial function and a preparation method and application thereof, which can effectively and physically isolate an anastomotic stoma from intestinal contents and provide a relatively clean local environment for healing of the anastomotic stoma. The microneedle anastomotic stent is fixed at the anastomotic stoma, so that the tension at the anastomotic stoma can be further reduced, and the occurrence of anastomotic stoma leakage caused by overlarge tension is avoided; the microneedle layer on the surface of the stent can realize in-situ long-acting slow release of berberine hydrochloride (BBH) on the superficial layer of an anastomotic stoma, so that the drug utilization rate of the BBH is improved, the initial inflammation of the anastomotic stoma can be effectively reduced, and the anastomotic stoma can be regulated and controlled to be better healed. Animal experiment results prove that the implantation of the microneedle anastomosis stent can effectively reduce inflammatory cell infiltration of local tissues, reduce anastomotic stoma arrangement and regulate the anastomotic stoma to heal smoothly.

The present invention relates to an intraoperative gastrointestinal decompressor, which includes a locator and a suction device detachably arranged inside the locator; the locator includes a vertically arranged positioning tube, a positioning ring sleeved and fixed outside the positioning tube, and at least two arc-shaped airbags arranged on the outer peripheral surface of the positioning tube and evenly distributed at equal angles along the circumferential direction; the suction device includes a vertically arranged hard tube, a base that is sealed and detachably fixed at the lower opening of the hard tube and located outside the lower end of the positioning tube, a hose that is hermetically connected to the upper opening of the hard tube and communicates with the inside of the hard tube, and a drainage tube that is hermetically inserted into the side surface of the lower end of the hard tube and communicates with the inside of the hard tube; the present invention reduces the operation difficulty and complexity, can effectively avoid intestinal injury, expands the applicable range and greatly improves the decompression effect; can effectively prevent the reflux of intestinal contents, effectively speeds up the operation progress, and improves the safety and success rate of the operation.

The present invention relates to methods of treating animals with an antimicrobial growth promoter (AGP) to promote a healthy microbiome, the method comprising: a) providing test organisms and a control organism, b) administering a regular regimen of a different AGP to each test organism for about two months, c) collecting DNA from the intestinal content of the test organisms and of the control organism, d) sequencing the DNA of the intestinal contents of the organisms to obtain taxonomic and functional profiles, thereby defining core intestinal microbiomes of the test and control organisms, e) comparing the core intestinal microbiomes of the test organisms and of the control organism, thereby determining which test organism has a healthy microbiome thereby determining which AGP provides the most healthy microbiome, and f) treating the domesticated organism with the AGP which provides the healthy microbiome.

The present invention discloses an intestinal flow field modeling method and device based on bidirectional fluid-solid coupling. The method comprises: S1, collecting intestinal multimodal data; wherein the multimodal data includes geometric parameters, motion characteristics, content statistics and wall stress and strain data of the intestine; S2, reconstructing a three-dimensional geometric model of the human intestine; S3, preprocessing the established intestinal geometric model by format conversion and simplification and modification; S4, dividing the preprocessed model into a fluid domain and a solid domain, and establishing a bidirectional fluid-solid coupling model architecture; S5, performing fluid domain simulation settings: simulating multiphase flow contents and setting a fluid simulation model; S6, performing solid domain simulation settings: setting intestinal wall material parameters and simulating intestinal movement; S7, achieving bidirectional fluid-solid coupling and simulating the intestinal flow field. The method takes into account the bidirectional interaction between the intestinal wall solid domain and the intestinal content fluid domain, achieving more accurate intestinal flow field simulation.

This utility model discloses a protective anal tube for rectal anastomosis after surgery, relating to the field of medical device technology. It solves the initial problem of the need to develop a protective anal tube for rectal anastomosis after surgery that has three-dimensional isolation, facilitates negative pressure drainage, and has a biological barrier function. The utility model includes a tube body with two support members on it for supporting the intestine. Both support members are capable of mechanical deformation, and the contact surfaces between the two support members and the intestine are annular, which can support both sides of the intestinal anastomosis. Thus, a cavity is formed between the two support members and the anastomotic segment of the intestine. The purpose is that the two support members form a physical barrier through the annular contact surfaces, completely preventing the leakage of intestinal contents into the anastomotic area, avoiding contamination of the wound by feces and digestive juices, maintaining a moist environment, and promoting the migration of epithelial cells and the growth of granulation tissue.

An ostomy bag device (102) includes an expandable reservoir (104) extending from a first end (110) to a second end (112) and defining a space therewithin. The device also includes first and second connectors (106, 108). The first connector is connected to the first end and engages an opening extending through a wall at an end of an intestine so that intestinal contents flowing from the end of the intestine are received and collected within the reservoir. The second connector is connected to the second end and engages an exit location via which contents collected within the reservoir are to be drained to an exterior of a patient body. The second connector includes a control element (122) movable between a closed configuration, in which contents of the reservoir are prevented from flowing therepast, and an open configuration, permitting contents to flow therepast to drain the reservoir.

The invention discloses an auxiliary device for jejunostomy nursing. The auxiliary device comprises an embedded air bag, a transition section and an outer side fixing disc. One end of the transition section is fixedly connected with the embedded air bag, and the other end of the transition section is fixedly connected with the outer side fixing disc; the embedded air bag is of an internally hollow inflatable air bag structure; the device can be embedded into the stoma of a patient to communicate the intestinal tract with the outside, the device can be expanded by adding gas or liquid so as to firmly fix the device and open the enteric cavity at the same time, and the device can also lift the opening of the stoma and turn intestinal contents, so that the nursing operation of the stoma is facilitated; and an ostomy bag can be better adhered, so that the skin of a patient is protected, and the risks of intestinal fistula and anastomotic fistula are reduced.

The invention provides pediococcus pentosaceus Su and application thereof, and belongs to the technical field of microorganisms. According to the invention, intestinal contents of horses and donkeys are screened to obtain a strain, the strain is identified as pediococcus pentosaceus, and the strain is mixed with lactobacillus plantarum to obtain the microbial inoculum. The microbial agent obtained by the invention can be used as an additive to improve the lactation yield of postpartum female donkeys and the nutritional quality of breast milk, increase the content of fat and improve the immunocompetence of donkey foals; the microbial inoculum can serve as a leavening agent to be fermented with motherwort, the obtained fermented motherwort serves as an additive, the optimized female donkey feed is obtained by optimizing a feed formula, the feed is used for feeding female donkeys, and the action effect of the microbial inoculum fermented motherwort in animal bodies is preliminarily understood.

The invention relates to the technical field of medical instruments, in particular to an intestinal obstruction catheter anti-reflux drainage device. The device comprises a three-way connector, a first drainage catheter communicated with a port in one end of the three-way connector, a spiral drainage tube communicated with a port in one end of the three-way connector, a one-way valve communicated with a port in one end of the three-way connector and a multi-cavity collection bag communicated with the spiral drainage tube. A plurality of elastic valves are arranged on the inner tube wall of the first drainage catheter in the circumferential direction of the first drainage catheter, each valve is provided with a pair of side edges and a lower edge, and the lower edges of the valves are fixedly connected with the inner wall of the first drainage catheter. According to the intestinal obstruction catheter anti-reflux drainage device, the problems that in existing intestinal obstruction drainage, intestinal contents in a drainage tube are prone to reflux, and the reflux solving operation is tedious are solved.

The invention discloses a closed enema system and an application method thereof, and belongs to the field of medical instruments, and the closed enema system comprises a perfusion pipeline used for being connected with a perfusion device for conveying enema liquid; the liquid discharging pipeline is used for being connected with a liquid discharging device for sucking intestinal contents; the outer wall of the drainage pipeline is sleeved with a turbidity sensor which is used for monitoring the turbidity of drainage liquid in real time; the filling pipeline and the liquid discharging pipeline are respectively communicated with the confluence pipeline; the controller is electrically connected with the turbidity sensor, the filling device and the liquid discharging device respectively; the controller is configured to receive monitoring data of the turbidity sensor in a liquid discharge stage and determine whether to start a next lavage cycle or not according to a comparison result of the data and a preset threshold value; and meanwhile, the filling device and the liquid discharging device are controlled to operate in a time-sharing manner. By means of the arrangement, preoperative clysis operation can be efficiently and rapidly completed in a patient-friendly mode.

The invention discloses a tunchang pig fat deposition related gene and microbial marker screening method and application, the method comprises the following steps: collecting fresh liver tissues and colon intestinal contents of high / low backfat thickness tunchang pigs, respectively carrying out liver transcriptome sequencing and intestinal microbial sequencing, and finding that the expression of CYP7A1 gene in the backfat pigs is obviously reduced, and the expression of CYP7A1 gene in the backfat pigs is obviously reduced; the gene is determined to be an important gene influencing the thickness of the Tonchang pig backfat by combining related analysis; the abundance of the Desulfovibrio flora in the intestinal tract of the high-backfat pig is obviously increased, and the Desulfovibrio flora is in obvious positive correlation with butyric acid. The invention discloses the effect of the CYP7A1 gene and Desulfovibrio flora abundance in regulating and controlling the fat deposition of the Trenchang pigs for the first time, provides candidate genes for molecular marker-assisted breeding of local pigs, and also provides a new idea for regulating and improving the fat deposition of the Trenchang pigs through microorganisms.

To provide an apparatus for treating a patient suffering from a disorder related to the passageway of the patient's intestines, such as constipation or incontinence.SOLUTION: An apparatus for treatment includes a pump to be implanted in a patient, where the pump is operable on at least one selected portion of the intestines to pump intestinal contents through the passageway of the intestines. The pump includes a constriction device adapted to alternately constrict the selected portion to at least substantially reduce the volume of the passageway of the intestines along the selected portion and release the selected portion to increase the volume of the passageway of the intestines along the selected portion, so that the intestinal contents are displaced through the passageway of the intestines.SELECTED DRAWING: Figure 2A

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The application discloses an intestinal micro-needle anastomosis stent with an antibacterial function and a preparation method and application thereof, which can effectively physically isolate an anastomotic stoma from intestinal contents, provides a relatively clean local environment for healing of the anastomotic stoma, and further reduces tension at the anastomotic stoma by fixing the micro-needle anastomosis stent at the anastomotic stoma, so that anastomotic leakage caused by excessive tension is avoided; a micro-needle layer on a surface of the stent can realize in-situ long-acting slow release of berberine hydrochloride (BBH) on a superficial layer of the anastomotic stoma, so that drug utilization of the BBH is improved, and occurrence of initial inflammation of the anastomotic stoma is effectively reduced, and healing of the anastomotic stoma is better regulated. Animal experiment results prove that implantation of the micro-needle anastomosis stent can effectively reduce inflammatory cell infiltration of local tissues, reduce anastomotic arrangement, and regulate smooth healing of the anastomotic stoma.

The invention relates to the technical field of stoma nursing, and discloses a stoma nursing assembly and a using method thereof.The stoma nursing assembly comprises a cleaning unit, an infusion tube and a pipette, the cleaning unit comprises buffer cotton, an infusion tube and a pipette, the buffer cotton completely covers a stoma wound surface, a through hole corresponding to an intestinal fistula opening is formed in the buffer cotton, and one end of the infusion tube and one end of the pipette are both inserted into the buffer cotton; the blocking and flow guiding unit is connected to the cleaning unit and used for isolating the intestinal fistula and the stoma wound surface; and the ostomy bag is connected to the plugging unit and used for collecting intestinal contents. According to the stoma nursing assembly and the using method thereof, the buffer cotton is attached to the wound surface of the stoma to fill a pit at the stoma, the buffer cotton and the skin jointly form a good installation base face, the ostomy bag is convenient to install, the ostomy bag is not prone to falling off after being installed, normal saline is injected into the buffer cotton through the infusion tube, and therefore the stoma nursing assembly is convenient to use. Normal saline in the buffer cotton is absorbed through the pipette, the wound surface of the stoma can be continuously cleaned, and the problem that the wound surface of the stoma is inconvenient to clean is solved.

The application discloses a method for improving the anti-cellular fibrosis of intestinal flora based on polysaccharides, and the steps of the method include: preparing Yunnan rhizoma polygonati mutant polysaccharide PKVP-1; and placing the Yunnan rhizoma polygonati mutant polysaccharide PKVP-1 in an intestinal environment to improve the anti-cellular fibrosis of intestinal flora. The steps of an experimental method for improving the anti-cellular fibrosis of intestinal flora based on polysaccharides include: selecting experimental animals and performing random grouping, and then performing drug administration; collecting the intestinal contents and kidney tissues of the experimental animals; taking the kidney tissues of the experimental animals to prepare paraffin sections for trichrome staining, and evaluating the inflammation degree of the kidney tissues according to the inflammatory cell infiltration; performing sequencing; detecting metabolites and obtaining a conclusion according to metabolite analysis. The method for improving the anti-cellular fibrosis of intestinal flora based on polysaccharides provided by the application improves the anti-cellular fibrosis ability of intestinal flora by placing the mutant polysaccharide in the intestinal environment of the experimental animals.

The invention discloses application of apolipoprotein H in preparation of drugs or health care products for preventing and / or treating alcoholic diseases. Researches show that an alcoholic hepatitis model is constructed by taking 10-week-old ApoH gene knockout mice (C57BL / 6ApoH- / -) as a background, a wild C57BL / 6 mouse alcoholic hepatitis model is taken as a contrast, peripheral blood transaminase and blood fat level, liver tissue fat content and liver tissue immunohistochemical staining are detected, and a result shows that the TC content of the liver tissue of the ApoH- / -mouse is obviously increased. The composition and content of bile acid in liver tissues and intestinal contents and the composition and abundance of intestinal flora are further detected, and results indicate that metabolic disorder of bile acid and disorder of intestinal flora exist. Based on the mechanism that low expression of alcoholic apolipoprotein H causes disorder of intestinal flora, affects bile acid metabolism and aggravates hepatic cell adiposis, the application is an accurate targeted therapeutic drug for treating alcoholic multi-organ injury.

The invention relates to lactobacillus plantarum and application thereof in laying hen fatty liver, the lactobacillus plantarum CK3 is obtained by being separated and screened from intestinal contents and excrement samples of Wenchang chicken in Hainan, is preserved in China Center for Type Culture Collection (CCTCC) on December 23, 2024, has the preservation number of CCTCC NO: M20242886, and has the preservation number of CCTCC NO: M20242886, the preservation number of CCTCC NO: M20242886, the preservation number of CCTCC NO: M20242886, the preservation number of CCTCC NO: M20242886, and the preservation number of CCTCC NO: M20242886. The laying hen feed additive has good tolerance in the environment with the temperature of 40-55 DEG C, the pH value of 2-4, 0.3% cholate and 1.4% trypsin, and can relieve laying hen liver lipid accumulation, liver inflammation and liver injury caused by high-energy feed, reduce liver antioxidant stress, remarkably improve laying hen fatty liver and improve laying hen immunity. Meanwhile, intestinal injury, intestinal inflammation and oxidative stress of the laying hens with the fatty liver caused by high-energy feed can be improved, and the feed has important application significance and value.

The present application relates to a kind of reagent and nanozyme immunochromatography test strip for detecting porcine rotavirus and its application, belong to biotechnology field, the present application is aimed at solving the technical problem of low sensitivity and poor specificity of existing porcine rotavirus detection method, its technical scheme main points are in containing capture antibody and detection antibody, the capture antibody is monoclonal antibody one, and the detection antibody is monoclonal antibody two, vice versa;Wherein the heavy chain variable region of monoclonal antibody one includes the sequence shown in SEQ ID NO:1, and the light chain variable region includes the sequence shown in SEQ ID NO:2;The heavy chain variable region of monoclonal antibody two includes the sequence shown in SEQ ID NO:5, and the light chain variable region includes the sequence shown in SEQ ID NO:6.The reagent can be used for preparing nanozyme immunochromatography test strip, realize the high sensitivity, high specificity detection of porcine rotavirus, suitable for the rapid detection of pig anal swab, feces or intestinal contents sample.