164 results about "Intestinal contents" patented technology

There is provided a method for controlling a flow of intestinal contents in the intestinal passageway of a patient's intestines. The method comprises gently constricting (i.e., without substantially hampering the blood circulation in the intestinal tissue wall) at least one portion of the intestinal tissue wall to influence the flow in the intestinal passageway, and stimulating the constricted wall portion to cause contraction of the wall portion to further influence the flow in the intestinal passageway. The method can be used for restricting or stopping the flow in the intestinal passageway, or for actively moving the fluid in the intestinal passageway, with a low risk of injuring the intestines.

The invention belongs to the technical field of endogenous substance detection and discloses a GC-MS (gas chromatography-mass spectrometer)-based method for quantifying eleven types of short-chain fatty acids in intestinal contents and fecal samples. By adoption of a hydrochloric acid solution prepared from saturated salt water for acidification, high-stability ethyl acetate for extraction and N-tert-butyldimethylsilyl-N-methyl trifluoroacetamide MTBSTFA for derivatization, the eleven short-chain fatty acids in mouse intestinal contents and fecal samples are quantified by a gas chromatography-mass spectrometer. The method is high in accuracy and precision and low in detection limit, can be used for quantitative detection of eleven types of short-chain fatty acids and can meet detection requirements on determination of major short-chain fatty acids in the mouse intestinal contents and the fecal samples. Moreover, the method is conducive to analysis and comparison of metabolic disposition laws of the short-chain fatty acids under normal and enteritis conditions and observation whether changes of the short-chain fatty acids can represent dynamic conditions of enteritis or not, thereby providing significant bases for potential biomarkers under the enteritis condition.

A disposable closure for an artificial intestinal or urethral opening in the form of a body having a cup form having an internal space being capable of accommodating a stoma, characterised in that the rim of the open end of the cup is provided with a flange part stretching essentially perpendicularly to the longitudinal direction of the closure and that the surface of the flange facing away from the closed end of the cup is provided with a skin-friendly adhesive may be used for safe temporary closing of a stoma without the risk of causing mechanical damage to the stomal wall and which prevents intestinal contents from passing the closure and which is easy to remove after use. It may also be used for protection of the outer surface of a stoma when applying a fresh collecting bag and furthermore as an aid for the user for an easier manner to place a fresh body side member or a one-piece receiving bag "correctly" ensuring a correct coupling and sealing.

The invention discloses Bifidobacterium animalis with a bowel relaxing function and application and a preparation of the Bifidobacterium animalis. According to the Bifidobacterium animalis, Bifidobacterium lactis BB-11 is separated from an intestinal content of healthy and long-lived elderly people in Bama, Guangxi, the strain is carried on a spacecraft, finally, a Bifidobacterium animalis MN-Gupwith significantly improved oxygen resistance or significantly enhanced oxygen resisting capacity is screened by space mutagenesis, and evaluation of constipation model mice proves that the strain hasthe good bowel relaxing function and has broad application prospects in bowel relaxing drugs, health foods and foods.

The invention discloses a negative-pressure automatic sampler of intestinal contents. The sampler comprises a shell, an inlet surface, a contraction neck, inlet holes, a supporting frame, a sealing resin block, sealing film and a protection support. The shell is in a microcapsule shape, one end of the shell is the inlet surface, and a vacuumizing pipe is arranged on one side of the shell. The diameter of the inlet surface is smaller than that of the shell by 2-4 mm, the shell is connected with the inlet surface through the contraction neck, the inlet holes are formed in the inlet surface, andthe supporting frame is arranged on the periphery of the inlet surface. The supporting frame is of a cylindrical frame structure and is fixedly connected with the contraction neck, the sealing resin block is arranged in the supporting frame, and the sealing film is arranged outside the supporting frame. The sealing resin block is in an oblate cylinder shape, the diameter of the sealing resin blockis smaller than that of the supporting frame by 2-4 mm, the height of the sealing resin block is smaller than that of the supporting frame by 2-4 mm, and the sealing resin block is made of water-absorbent resin. The sealing film is in a semispherical housing shape, and the lower circular frame of the supporting frame is sealed by the circular edge of the sealing film. The protection support is arranged on the outer layer of the sealing film, is composed of a circular bottom edge and 3-8 arc supporting rods, and is fixedly connected with the contraction neck. The vacuumizing pipe is hidden inside, is communicated with the inner cavity of the sampler and is sealed after vacuumizing is conducted.

A method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement comprises the following steps: (1) carrying out homogeneous dilution by using intestinal contents or distilled water for excrement at the ratio of 1:9-1:14, uniformly mixing, centrifuging a mixture for 15-20 min, and taking 0.5-1 ml of a supernatant; (2) carrying out chromatographic analysis through Agilent 6890 N gas chromatography and an HP-INNOWAX chromatographic column; gas phase analysis conditions: an FID detector is used and carrier gas is N2; N2 flow velocity is 19.0ml/min, and split ratio is 1:10; air velocity is 300 ml/min, and H2 flow velocity is 30 ml/min; detector temperature is 240 DEG C, and injection port temperature is 240 DEG C; temperature programming: after maintaining 100 DEG C for 0.5 min, heating to 150 DEG C at the temperature rising speed of 4 DEG C/min, and measuring for 13 min; inlet sample quantity is 0.2-0.5 microliter, 3 times of separate repeated measurements are carried out for each sample for each time, and data analysis is carried out by HP Chem workstation software. Only after homogenizing a sample in distilled water and centrifuging the sample, a supernatant is taken to directly carry out gas phase sample-introduction and analysis, and measurement time can be reduced remarkably. In addition, the method is simple, rapid and accurate.

The invention relates to a specific probe and a method thereof for rapid and sensitive quantification of staphylococcus aureus. On the basis of the sequence of a 16s-rRNA conservation terminal of the staphylococcus aureus, a specific complementary nucleic acid probe is designed, the probe is named as SA-1 and 5' terminal is labeled with fluorescein isothiocyanate FITC; and the sequence number of the SA-1 is shown as 5'-GCCGGTGGAGTAACCTTTTAGGAGC-3'. According to the invention, the specific complementary nucleic acid probe is designed on the basis of the sequence of the 16s-rRNA conservation terminal of the staphylococcus aureus and is named as SA-1, the 5' terminal is labeled with fluorescein isothiocyanate FITC, and the FIFC-labeled SA-1 can achieve specific combination with the processed staphylococcus aureus so as to excite fluorescent light from the staphylococcus aureus; and on the basis, propidium iodide PI is adopted for overall dyeing treatment on bacteria, sarcoid cells or intestinal contents, and fluorescence parameters are analyzed by virtue of a flow cytometry by taking a fluorescent microsphere with known concentration as internal reference, so that the purpose of quantifying bacteria is achieved.

The invention discloses an intraoperative intestinal decompression suction device, which comprises a hose, a hard tube joint arranged at the inlet end of the hose, a tube sleeve, a sewage collecting bottle, an exhaust tube and a negative pressure suction unit, wherein one end of the tube sleeve is fixedly sleeved on the hard tube joint, and the other end of the tube sleeve is arranged freely; the tube sleeve can be folded towards two ends of the hard tube joint; a joint I connected with the hose and a joint II connected with the exhaust tube are arranged on the sewage collecting bottle; and the exhaust tube is connected with an air inlet of the negative pressure suction unit. According to the intraoperative intestinal decompression suction device, the hard tube joint is connected with an intestinal tube, the intestinal tube is bound on the hard tube joint, and the tube sleeve is folded to be bound on the intestinal tube; the tube sleeve can collect intestinal contents leaked from the juncture of the intestinal tube and the hard tube joint; the hose is relatively long, and sewage is convenient to discharge, so that the intestinal contents can be prevented from infecting abdominal cavities and operative incisions of patients; and the negative pressure suction unit can suck odor in the sewage collecting bottle and make the inside of the sewage collecting bottle be in the negative pressure state, so that the intestinal contents enter the sewage collecting bottle smoothly.

The invention belongs to the technical field of preparation of veterinary microbial additives and particularly relates to screening and application of Clostridium butyricum for balancing intestinal flora of livestock and poultry. The Clostridium butyricum is characterized in that the Clostridium butyricum HDRyYB1 is isolated from intestinal contents of goats, and experiments show that the Clostridium butyricum has an evident function of inhibiting intestinal pathogenic bacteria and is capable of promoting proliferation of beneficial bacteria; the Clostridium butyricum is preserved in China Center for Type Culture Collection under CCTCC NO: M2015200. The Clostridium butyricum has the advantages of high growth speed, high stress resistance, safety, disease resistance, capability of improving growth performance and the like; the Clostridium butyricum is applicable to the preparation of microbial additives for livestock and poultry.

There is provided a method for controlling a flow of intestinal contents in the intestinal passageway of a patient's intestines. The method comprises gently constricting (i.e., without substantially hampering the blood circulation in the intestinal tissue wall) at least one portion of the intestinal tissue wall to influence the flow in the intestinal passageway, and stimulating the constricted wall portion to cause contraction of the wall portion to further influence the flow in the intestinal passageway. The method can be used for restricting or stopping the flow in the intestinal passageway, or for actively moving the fluid in the intestinal passageway, with a low risk of injuring the intestines.

The invention provides glutinous rice dumpling sausage, and relates to sausage, which is characterized in that the glutinous rice dumpling sausage contains rice, animal fat fiber, ice crystal, starch, edible gelatin, added trace element and vitamin as reinforcing agents and proper amount of monosodium glutamate; and the weight percentage of the raw materials is: 20-50 of rice, 10-14 of animal fat fiber, 14-24 of ice crystal, 14-22 of starch, 7-12 of edible gelatin, 5-8 of reinforcing agents and 0.1-1 of monosodium glutamate. The invention has advantages that the glutinous rice dumpling sausage can be produced industrially and has long storage life and novel formula, filling material of the sausage contains a large amount of vitamin, protein, amino acid and mineral matter, and the glutinous rice dumpling sausage has special taste, can keep health, nourish brain and develop intelligence, and is suitable for all people.

The invention relates to an intestinal content separation and recycling device and method and a product thereof. The device comprises an impurity removal module 1 and a target content collection module 2, wherein the target content collection module comprises a micro-pore membrane filtering net 5; after excrement is mixed with a solvent (such as normal saline), impurities are firstly removed by utilizing the impurity removal module 1, filtration is implemented by utilizing the micro-pore membrane filtering net 5 secondly, and target contents are collected from the micro-pore membrane filteringnet 5. By utilizing a micro-pore membrane technique, intestinal microorganisms are separated and extracted, a conventional centrifugal separation technique is replaced, firstly, an extraction processcan be precise and controllable, high in separation efficiency and meanwhile simple, convenient to operate and high in automation degree, secondly, micro-pore membranes of different apertures can beused, due to a size effect, expected intestinal microorganisms can be selectively separated, and relative abundance of different bacteria can be precisely controlled.

The invention discloses a disposable intestinal content extracting device, comprising a support, a filter unit arranged on the support, and a pretreatment device connected with the filter unit. The disposable intestinal content extracting device has the advantages that, feces can be diluted to be feces suspension liquid and the feces bacteria is filtered and separated under the anaerobic condition, operation is convenient, medical staffs are easy to accept, quality of the feces bacteria and the disease treatment effect are improved.

The invention discloses an intestinal double-cavity fistulization excrement transferring device. The device comprises a bent pipe, the bent pipe is made of rubber, and air bags are arranged on the outer sides of two ends of the bent pipe in a surrounding mode; the air bags are connected with inflation pipes, a one-way valve is arranged at one end of the bent pipe, and a transparent cover is arranged on the outer side wall of the corner of the bent pipe. After systematic inspection, an intestinal stoma reduction operation is not conducted vexedly, the intestinal double-cavity fistulization excrement transferring device is used for continuing inspection, and with the assistance of the bridge connection effect of the transferring device, contents of an enteric cavity at the near end of a stoma pass through the transferring device and then enter an enteric cavity at the far end of the stoma; physiological status of an intestinal tract after the stoma reduction operation is simulated, the reaction of the original far-end diseased intestinal tract under the stimulus of the intestinal contents is observed, and when it is determined that pathological factors of the original far-end intestinal tract are completely removed, and then the intestinal stoma reduction operation is conducted. The situation is avoided that a patient is also compelled to be subjected to a near-end intestinal fistulization operation due to original disease recurrence of the far-end intestinal tract after the stoma reduction operation and accordingly the body and spirit of the patient get hurt.

The invention discloses a method for determining short chain fatty acid content in intestinal contents or excrement. A sample is pretreated by diethyl ether, and the short chain fatty acid content inthe sample is determined through a gas chromatographic method; and pretreatment temperature is 2-8 DEG C. In the method provided by the invention, other derivatization pretreatment steps are not needed, pretreatment time is less than 15min, length of determining time is less than 20min; low temperature can effectively reduce volatilization loss of the short chain fatty acid, and recovery rate is up to 98.10 +/-0.29%-101.30 +/-0.27%; with the method provided by the invention, limit of detection can be as low as 0.0036 <mu>L/mL, limit of quantitation can be as low as 0.0041 <mu>L/mL, so the method is more accurate and efficient for detection of the short chain fatty acid.

The invention discloses a sow feed additive for promoting growth and health of weaning pigs. The feed additive disclosed by the invention is prepared by mixing all substances in parts by weight: 2 parts of enterococcus faecium, 2-3 parts of lysozyme and 95-96 parts of a carrier, wherein the carrier is starch; the viable count in the enterococcus faecium is 1.0*10<11>cfu/g; the enzyme activity of the lysozyme is 5,000,000IU/g. According to the sow feed additive, probiotics and the lysozyme serving as a natural immune component of an organism are combined so as to cooperatively promote multiplication of beneficial bacteria in the intestinal tracts of sows, reduce the amount of harmful bacteria such as escherichia coli, maintain and regulate the balance of intestinal flora of the sows, increase the number of beneficial bacteria in intestinal contents and excrements, alleviate the action caused by the harmful bacteria, purify the environments in vivo and in vitro, create a good flora environment for new-born piglets and achieve the effects of increasing the weaning weights of the piglets, increasing the weights of the pigs weaned for 3 weeks and reducing the diarrhea occurrence rate. Furthermore, the formula of the feed additive does not contain medicaments, so that drug resistance and drug residues can be avoided.

The present invention relates to a pure Chinese medicine granule preparation for treating gastroesophageal reflux disease. The gastroesophageal reflux disease is a common clinical disease, referring to a syndrome caused by the reflux of excessive stomach and duodeno contents into oesophagus. The present invention includes the following ingredients: ruddle, radix bupleuri, Chinese goldthread, evodia rutaecarpa, largehead atractylode, pachyma cocos, hovenia dulcis and zhibanxia, which according to a certain proportion are processed into a pure Chinese medicine granule preparation through certain procedures for treating gastroesophageal reflux disease. The present invention has the advantages of good curative effect, convenience and low price, no adverse side effects.

The invention discloses a method for separating, screening and identifying earthworm gut culturable antibiotic resistant bacteria. The method comprises the following steps: selecting fresh earthwormswhich have obvious clitellum and of which the weights are in a certain range, cleaning the body surface, performing anesthesia, dissecting, and taking intestinal contents; diluting the intestinal contents into a certain concentration gradient with sterile water, coating the diluents of different concentrations on an improved LB solid plate containing antibiotics of different types and contents, and culturing, screening and purifying the bacteria; culturing the purified bacterial colony in an improved LB liquid medium, and then extracting DNA by using a bacterial DNA extraction kit; performing16S rRNA amplification on the extracted DNA, and detecting the amplification product by using gel electrophoresis having the mass fraction of 2%; and performing sequencing analysis on the amplification product, and carrying out comparison on the NCBI after obtaining the sequence.

The invention discloses a pretreatment method for extracting metagenome DNA of intestinal contents. The pretreatment method comprises the following steps of: adding a necrotic-tissue removing agent into the intestinal contents to wash for twice, then adding nuclease eliminating solution for washing, cracking cells by adopting a freezing-thawing method and a cracking method, fully releasing DNA in the cells, finally adding protease K, SDS and RNA enzyme to remove protein, fiber and RNA in the cells, and treating products by adopting a conventional method to obtain the metagenome DNA of the intestinal contents. The pretreatment method disclosed by the invention has the advantages that the effect for extracting the DNA of the metagenome DNA of the intestinal contents of omnivorous animals is obvious, and both the extracting purity and the extracting amount can meet downstream experiments such as PCR augmentation and high-throughput sequencing and the like, so that the practicability is very strong.

The invention discloses a pressure-reducing collecting device used in intestinal obstruction operations. The pressure-reducing collecting device comprises a tubular body, the body comprises a first channel, the front end of the body is fixed inside an intestinal tube, the rear end of the body is fixedly connected with a waste containing bag, the body is further provided with a second channel, the first channel is communicated with the second channel, a flushing pipe can be inserted into the second channel selectively, the front end of the flushing pipe sequentially penetrates through the second channel and the first channel to stretch out of the front end of the first channel, and the front end of the flushing pipe is inserted into the obstruction part suffering from intestinal obstruction. The pressure-reducing collecting device mainly has the advantages that when the pressure-reducing collecting device which can be selectively provided with the flushing pipe is in use, the flushing pipe is inserted into the content with obstruction, then irrigation flushing is conducted, the intestinal content with obstruction can be smashed and diluted better, and therefore the content can be discharged out conveniently.

The invention discloses a method for detecting virus from a poultry disease material, and application of the method. The method comprises the following steps: sterilely acquiring tissues of detected dead poultry such as intestinal contents, lung, air chamber, intestines, spleen, liver or heart, or sterilely acquiring blood, trachea swab, cloacae swab or excrement of detected living poultry; extracting RNA from a sample by adopting a Trizol method or a Mini BEST virus RNA extraction kit; using the extracted RNA as a template to carry out an RT-LAMP reaction with upstream and downstream primers at the outside and upstream and downstream primers at the inside, sampling the reactant after the RT-LAMP reaction is finished to carry out agarose gel electrophoresis detection, and judging whether the detected poultry are infected by H9 type avian influenza virus by a way that whether a ladder type band with RT-LAMP reaction specificity appears.