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164 results about "Intestinal contents" patented technology
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The consistency of the intestinal contents changes gradually as the contents travel through the small intestine. In the duodenum, food is diluted with pancreatic enzymes and bile, which decrease stomach acidity.
Method for controlling flow of intestinal contents in a patient's intestines
ActiveUS20090240100A1Eliminate the problemAnti-incontinence devicesSurgical needlesSurgeryBlood circulating
There is provided a method for controlling a flow of intestinal contents in the intestinal passageway of a patient's intestines. The method comprises gently constricting (i.e., without substantially hampering the blood circulation in the intestinal tissue wall) at least one portion of the intestinal tissue wall to influence the flow in the intestinal passageway, and stimulating the constricted wall portion to cause contraction of the wall portion to further influence the flow in the intestinal passageway. The method can be used for restricting or stopping the flow in the intestinal passageway, or for actively moving the fluid in the intestinal passageway, with a low risk of injuring the intestines.
Owner:FORSELL PETER
Preparation method of novel protein feed additive
ActiveCN101530162AIncrease egg productionExtend peak egg productionAnimal feeding stuffAccessory food factorsBiotechnologyAnimal science
The invention relates to a preparation method of a hydrolyzed protein mixture and a protein peptide powder feed additive, wherein a multienzyme complex enzyme method is used for hydrolyzing vegetable protein to prepare the hydrolyzed protein mixture and protein peptide powder. The technology of the method is simple, and a non-starch polysaccharide enzyme and a proteinase are compounded so as to be capable of improving the hydrolyzation rate and degree of protein as well as the utilization rate of feed and reducing the production cost. Products prepared by the preparation method can be used as the feed additive to be capable of significantly improving the laying rate of egg-laying poultry, improving the weight increment of meat poultry, reducing the feed-meat rate and improving the production performance of table poultry as well as reducing the incidence rate of diarrhea in young animals and the feed-meat rate of piggy; and the preparation method is applied to aquatic livestock to be capable of improving the weight increment of fish and reducing the bait coefficient and content viscosity in the intestinal tract.
Owner:BEIJING DABEINONG TECH GRP CO LTD +1
GC-MS (gas chromatography-mass spectrometer)-based method for quantifying eleven types of short-chain fatty acids in intestinal contents and fecal samples
InactiveCN105651908AHigh extraction recoveryImprove securityComponent separationGas phasePotential biomarkers
The invention belongs to the technical field of endogenous substance detection and discloses a GC-MS (gas chromatography-mass spectrometer)-based method for quantifying eleven types of short-chain fatty acids in intestinal contents and fecal samples. By adoption of a hydrochloric acid solution prepared from saturated salt water for acidification, high-stability ethyl acetate for extraction and N-tert-butyldimethylsilyl-N-methyl trifluoroacetamide MTBSTFA for derivatization, the eleven short-chain fatty acids in mouse intestinal contents and fecal samples are quantified by a gas chromatography-mass spectrometer. The method is high in accuracy and precision and low in detection limit, can be used for quantitative detection of eleven types of short-chain fatty acids and can meet detection requirements on determination of major short-chain fatty acids in the mouse intestinal contents and the fecal samples. Moreover, the method is conducive to analysis and comparison of metabolic disposition laws of the short-chain fatty acids under normal and enteritis conditions and observation whether changes of the short-chain fatty acids can represent dynamic conditions of enteritis or not, thereby providing significant bases for potential biomarkers under the enteritis condition.
Owner:CHINA PHARM UNIV
Device for extracting target component of intestinal content
ActiveCN104212708AQuality improvementImprove the effect of treatmentApparatus sterilizationBiomass after-treatmentIntensive care medicineIntestino-intestinal
The invention provides a device for extracting the target component of an intestinal content. The device comprises a base, a support frame, a vibration device, an extraction container and a negative pressure device, wherein the vibration device is arranged on the support frame; the extraction container is movably connected with the vibration device; and the negative pressure device is connected with the extraction container. The device provided by the invention is convenient to operate and easy to accept by medical staff by integrating fecal suspension liquid preparation and caprophyl extraction and enhances the caprophyl quality and the disease treatment effect.
Owner:广州益可医生物科技有限公司
Ostomy sealing device
A disposable closure for an artificial intestinal or urethral opening in the form of a body having a cup form having an internal space being capable of accommodating a stoma, characterised in that the rim of the open end of the cup is provided with a flange part stretching essentially perpendicularly to the longitudinal direction of the closure and that the surface of the flange facing away from the closed end of the cup is provided with a skin-friendly adhesive may be used for safe temporary closing of a stoma without the risk of causing mechanical damage to the stomal wall and which prevents intestinal contents from passing the closure and which is easy to remove after use. It may also be used for protection of the outer surface of a stoma when applying a fresh collecting bag and furthermore as an aid for the user for an easier manner to place a fresh body side member or a one-piece receiving bag "correctly" ensuring a correct coupling and sealing.
Owner:COLOPLAST AS
Medical equipment and application thereof
ActiveCN103315835AAdaptableImprove self-cleaning abilityNon-surgical orthopedic devicesObesity treatmentDiabetes mellitusMedical equipment
Owner:万平
Bifidobacterium animalis with bowel relaxing function as well as application and preparation of bifidobacterium animalis
ActiveCN111826299AGood laxative functionImprove oxygen resistanceOrganic active ingredientsBacteriaBiotechnologyIntestino-intestinal
The invention discloses Bifidobacterium animalis with a bowel relaxing function and application and a preparation of the Bifidobacterium animalis. According to the Bifidobacterium animalis, Bifidobacterium lactis BB-11 is separated from an intestinal content of healthy and long-lived elderly people in Bama, Guangxi, the strain is carried on a spacecraft, finally, a Bifidobacterium animalis MN-Gupwith significantly improved oxygen resistance or significantly enhanced oxygen resisting capacity is screened by space mutagenesis, and evaluation of constipation model mice proves that the strain hasthe good bowel relaxing function and has broad application prospects in bowel relaxing drugs, health foods and foods.
Owner:INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
Continent ostomy system with chemical neuromuscular control
InactiveUS20090275795A1Arresting advancementReduces prograde and retrograde migrationColostomyProsthesisSmooth musclePeristalsis
An apparatus for providing continence to a gastrointestinal ostomy of a patient, wherein the apparatus includes a lumen sealing device, a chemical neuromuscular control agent, and a mechanism for controlled, localized delivery of the neuromuscular control agent. The lumen sealing device is positioned in contact with the mucosal wall of the intestine and the neuromuscular control agent is delivered to the intestine via the mechanism for controlled, localized delivery, such that the neuromuscular control agent provides an inhibitory effect on peristalsis or smooth muscle contraction and relaxation cycles in the intestine to thereby arrest the advancement of the contents of the intestine.
Owner:ZASSI MEDICAL EVOLUTIONS
Negative-pressure automatic sampler of intestinal contents
ActiveCN107970045AImprove mechanical propertiesGood physical propertiesSurgeryVaccination/ovulation diagnosticsEngineeringIntestinal contents
Owner:SHANXI AGRI UNIV
Method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement
InactiveCN105021726AReduced measurement timeAnalysis method is simpleComponent separationAir velocityInjection port
A method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement comprises the following steps: (1) carrying out homogeneous dilution by using intestinal contents or distilled water for excrement at the ratio of 1:9-1:14, uniformly mixing, centrifuging a mixture for 15-20 min, and taking 0.5-1 ml of a supernatant; (2) carrying out chromatographic analysis through Agilent 6890 N gas chromatography and an HP-INNOWAX chromatographic column; gas phase analysis conditions: an FID detector is used and carrier gas is N2; N2 flow velocity is 19.0ml / min, and split ratio is 1:10; air velocity is 300 ml / min, and H2 flow velocity is 30 ml / min; detector temperature is 240 DEG C, and injection port temperature is 240 DEG C; temperature programming: after maintaining 100 DEG C for 0.5 min, heating to 150 DEG C at the temperature rising speed of 4 DEG C / min, and measuring for 13 min; inlet sample quantity is 0.2-0.5 microliter, 3 times of separate repeated measurements are carried out for each sample for each time, and data analysis is carried out by HP Chem workstation software. Only after homogenizing a sample in distilled water and centrifuging the sample, a supernatant is taken to directly carry out gas phase sample-introduction and analysis, and measurement time can be reduced remarkably. In addition, the method is simple, rapid and accurate.
Owner:NANCHANG UNIV
Disposable extraction device of target components of intestinal contents
InactiveCN104212707AIntegrity guaranteedSimple structureApparatus sterilizationBiomass after-treatmentBiotechnologyMedicine
The invention discloses a disposable extraction device of target components of intestinal contents. The disposable extraction device comprises an extraction pipe, wherein the extraction pipe comprises a pipe body and a plurality of stages of filtering nets, wherein the upper end of the pipe body is provided with an opening and can be subjected to heat seal; the filtering nets are connected with the inner wall of the pipe body in a sealing manner; the pipe body also comprises liquid injection ends and liquid suction ends which are arranged on the lateral walls of the pipe body, wherein the liquid injection ends are positioned at the opening in the upper end and the first stage of filtering net, and the liquid suction ends are positioned between every two adjacent stages of filtering nets; the apertures of each filtering net from top to bottom have the trend of reducing stage by stage; the pipe body is made of a flexible high polymer material. The disposable extraction device disclosed by the invention has the beneficial effects that the structure is simple, the operation is convenient, an oxygen-free environment is provided for coprophilous fungi, the integrity of the coprophilous fungi can be guaranteed and the effect of the transplanted coprophilous fungi for treating diseases can be improved.
Owner:QINGDAO MUNICIPAL HOSPITAL
Specific probe and method thereof for rapid and sensitive quantification of staphylococcus aureus
InactiveCN105506070AImprove work efficiencyReduce distractionsMicrobiological testing/measurementMicroorganism based processesNucleic Acid ProbesStaphylococcus aureus
The invention relates to a specific probe and a method thereof for rapid and sensitive quantification of staphylococcus aureus. On the basis of the sequence of a 16s-rRNA conservation terminal of the staphylococcus aureus, a specific complementary nucleic acid probe is designed, the probe is named as SA-1 and 5' terminal is labeled with fluorescein isothiocyanate FITC; and the sequence number of the SA-1 is shown as 5'-GCCGGTGGAGTAACCTTTTAGGAGC-3'. According to the invention, the specific complementary nucleic acid probe is designed on the basis of the sequence of the 16s-rRNA conservation terminal of the staphylococcus aureus and is named as SA-1, the 5' terminal is labeled with fluorescein isothiocyanate FITC, and the FIFC-labeled SA-1 can achieve specific combination with the processed staphylococcus aureus so as to excite fluorescent light from the staphylococcus aureus; and on the basis, propidium iodide PI is adopted for overall dyeing treatment on bacteria, sarcoid cells or intestinal contents, and fluorescence parameters are analyzed by virtue of a flow cytometry by taking a fluorescent microsphere with known concentration as internal reference, so that the purpose of quantifying bacteria is achieved.
Owner:JIANGSU INST OF POULTRY SCI
Intraoperative intestinal decompression suction device
The invention discloses an intraoperative intestinal decompression suction device, which comprises a hose, a hard tube joint arranged at the inlet end of the hose, a tube sleeve, a sewage collecting bottle, an exhaust tube and a negative pressure suction unit, wherein one end of the tube sleeve is fixedly sleeved on the hard tube joint, and the other end of the tube sleeve is arranged freely; the tube sleeve can be folded towards two ends of the hard tube joint; a joint I connected with the hose and a joint II connected with the exhaust tube are arranged on the sewage collecting bottle; and the exhaust tube is connected with an air inlet of the negative pressure suction unit. According to the intraoperative intestinal decompression suction device, the hard tube joint is connected with an intestinal tube, the intestinal tube is bound on the hard tube joint, and the tube sleeve is folded to be bound on the intestinal tube; the tube sleeve can collect intestinal contents leaked from the juncture of the intestinal tube and the hard tube joint; the hose is relatively long, and sewage is convenient to discharge, so that the intestinal contents can be prevented from infecting abdominal cavities and operative incisions of patients; and the negative pressure suction unit can suck odor in the sewage collecting bottle and make the inside of the sewage collecting bottle be in the negative pressure state, so that the intestinal contents enter the sewage collecting bottle smoothly.
Owner:THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
Screening and application of Clostridium butyricum for balancing intestinal flora of livestock
ActiveCN105062914AIncrease production capacityStrong stress resistanceBacteriaMicroorganism based processesDiseaseMicroorganism
The invention belongs to the technical field of preparation of veterinary microbial additives and particularly relates to screening and application of Clostridium butyricum for balancing intestinal flora of livestock and poultry. The Clostridium butyricum is characterized in that the Clostridium butyricum HDRyYB1 is isolated from intestinal contents of goats, and experiments show that the Clostridium butyricum has an evident function of inhibiting intestinal pathogenic bacteria and is capable of promoting proliferation of beneficial bacteria; the Clostridium butyricum is preserved in China Center for Type Culture Collection under CCTCC NO: M2015200. The Clostridium butyricum has the advantages of high growth speed, high stress resistance, safety, disease resistance, capability of improving growth performance and the like; the Clostridium butyricum is applicable to the preparation of microbial additives for livestock and poultry.
Owner:HUAZHONG AGRI UNIV +2
Method for controlling flow of intestinal contents in a patient's intestines
ActiveUS20150359617A1Eliminate the problemAnti-incontinence devicesSurgical needlesSurgeryBlood circulating
There is provided a method for controlling a flow of intestinal contents in the intestinal passageway of a patient's intestines. The method comprises gently constricting (i.e., without substantially hampering the blood circulation in the intestinal tissue wall) at least one portion of the intestinal tissue wall to influence the flow in the intestinal passageway, and stimulating the constricted wall portion to cause contraction of the wall portion to further influence the flow in the intestinal passageway. The method can be used for restricting or stopping the flow in the intestinal passageway, or for actively moving the fluid in the intestinal passageway, with a low risk of injuring the intestines.
Owner:FORSELL PETER
Sausage filled with glutinous rice and meat
InactiveCN101449835AFit for consumptionExtended shelf lifeFood preparationMonosodium glutamateFilling materials
The invention provides glutinous rice dumpling sausage, and relates to sausage, which is characterized in that the glutinous rice dumpling sausage contains rice, animal fat fiber, ice crystal, starch, edible gelatin, added trace element and vitamin as reinforcing agents and proper amount of monosodium glutamate; and the weight percentage of the raw materials is: 20-50 of rice, 10-14 of animal fat fiber, 14-24 of ice crystal, 14-22 of starch, 7-12 of edible gelatin, 5-8 of reinforcing agents and 0.1-1 of monosodium glutamate. The invention has advantages that the glutinous rice dumpling sausage can be produced industrially and has long storage life and novel formula, filling material of the sausage contains a large amount of vitamin, protein, amino acid and mineral matter, and the glutinous rice dumpling sausage has special taste, can keep health, nourish brain and develop intelligence, and is suitable for all people.
Owner:TIANJIN ZHONGYING HEALTH FOOD
Intestinal content separation and recycling device and method and product thereof
PendingCN108676704AAvoid cloggingExtend your lifeBioreactor/fermenter combinationsBiological substance pretreatmentsFiltrationIntestinal microorganisms
The invention relates to an intestinal content separation and recycling device and method and a product thereof. The device comprises an impurity removal module 1 and a target content collection module 2, wherein the target content collection module comprises a micro-pore membrane filtering net 5; after excrement is mixed with a solvent (such as normal saline), impurities are firstly removed by utilizing the impurity removal module 1, filtration is implemented by utilizing the micro-pore membrane filtering net 5 secondly, and target contents are collected from the micro-pore membrane filteringnet 5. By utilizing a micro-pore membrane technique, intestinal microorganisms are separated and extracted, a conventional centrifugal separation technique is replaced, firstly, an extraction processcan be precise and controllable, high in separation efficiency and meanwhile simple, convenient to operate and high in automation degree, secondly, micro-pore membranes of different apertures can beused, due to a size effect, expected intestinal microorganisms can be selectively separated, and relative abundance of different bacteria can be precisely controlled.
Owner:QIHUI BIOTECHNOLOGY YANGZHOU CO LTD
Disposable intestinal content extracting device
InactiveCN104814767AQuality improvementImprove the effect of treatmentSurgeryVaccination/ovulation diagnosticsFecesDisease treatment
The invention discloses a disposable intestinal content extracting device, comprising a support, a filter unit arranged on the support, and a pretreatment device connected with the filter unit. The disposable intestinal content extracting device has the advantages that, feces can be diluted to be feces suspension liquid and the feces bacteria is filtered and separated under the anaerobic condition, operation is convenient, medical staffs are easy to accept, quality of the feces bacteria and the disease treatment effect are improved.
Owner:卢士海
Cascade analysis for intestinal contraction detection
Owner:GIVEN IMAGING LTD
Bacillus and application thereof
ActiveCN113652373AAdjustable micro-ecological balancePromote digestion and absorptionBacteriaDigestive systemBiotechnologyAmylase
The invention relates to a bacillus and application thereof. The bacillus coagulans is screened from intestinal contents of healthy piglets, the preservation number of the bacillus coagulans is CGMCC 22951, and the bacillus coagulans can produce acid, amylase and protease, is high in gastric acid resistance and cholate resistance and good in thermal stability, and can grow rapidly at the intestinal temperature of -39 DEG C of livestock and poultry. The invention further provides an application of the bacillus in facultative co-fermentation with clostridium butyricum. Through a high-temperature (39 DEG C) co-fermentation system, the cooling cost is reduced on one hand, and the growth of impurities are effectively inhibited on the other hand, so that the fermentation process is simplified; the newly screened strains grow faster under the facultative oxygen condition, so that the viable bacterium concentration and the spore concentration of the clostridium butyricum in a co-fermentation system are higher, and a new energy-saving and environment-friendly mode is provided for production of the clostridium butyricum.
Owner:HENAN JINBAIHE BIOTECH CO LTD +1
Intestinal double-cavity fistulization excrement transferring device
The invention discloses an intestinal double-cavity fistulization excrement transferring device. The device comprises a bent pipe, the bent pipe is made of rubber, and air bags are arranged on the outer sides of two ends of the bent pipe in a surrounding mode; the air bags are connected with inflation pipes, a one-way valve is arranged at one end of the bent pipe, and a transparent cover is arranged on the outer side wall of the corner of the bent pipe. After systematic inspection, an intestinal stoma reduction operation is not conducted vexedly, the intestinal double-cavity fistulization excrement transferring device is used for continuing inspection, and with the assistance of the bridge connection effect of the transferring device, contents of an enteric cavity at the near end of a stoma pass through the transferring device and then enter an enteric cavity at the far end of the stoma; physiological status of an intestinal tract after the stoma reduction operation is simulated, the reaction of the original far-end diseased intestinal tract under the stimulus of the intestinal contents is observed, and when it is determined that pathological factors of the original far-end intestinal tract are completely removed, and then the intestinal stoma reduction operation is conducted. The situation is avoided that a patient is also compelled to be subjected to a near-end intestinal fistulization operation due to original disease recurrence of the far-end intestinal tract after the stoma reduction operation and accordingly the body and spirit of the patient get hurt.
Owner:SICHUAN CANCER HOSPITAL
Method for determining short chain fatty acid content in intestinal contents or excrement
InactiveCN110045040AReduce volatile lossLow detection limitComponent separationDiethyl etherDerivatization
The invention discloses a method for determining short chain fatty acid content in intestinal contents or excrement. A sample is pretreated by diethyl ether, and the short chain fatty acid content inthe sample is determined through a gas chromatographic method; and pretreatment temperature is 2-8 DEG C. In the method provided by the invention, other derivatization pretreatment steps are not needed, pretreatment time is less than 15min, length of determining time is less than 20min; low temperature can effectively reduce volatilization loss of the short chain fatty acid, and recovery rate is up to 98.10 + / -0.29%-101.30 + / -0.27%; with the method provided by the invention, limit of detection can be as low as 0.0036 <mu>L / mL, limit of quantitation can be as low as 0.0041 <mu>L / mL, so the method is more accurate and efficient for detection of the short chain fatty acid.
Owner:JIANGNAN UNIV
Sow feed additive for promoting growth and health of weaning pigs
InactiveCN103750052APromote proliferationReduce in quantityAnimal feeding stuffFood additiveEscherichia coli
The invention discloses a sow feed additive for promoting growth and health of weaning pigs. The feed additive disclosed by the invention is prepared by mixing all substances in parts by weight: 2 parts of enterococcus faecium, 2-3 parts of lysozyme and 95-96 parts of a carrier, wherein the carrier is starch; the viable count in the enterococcus faecium is 1.0*10<11>cfu / g; the enzyme activity of the lysozyme is 5,000,000IU / g. According to the sow feed additive, probiotics and the lysozyme serving as a natural immune component of an organism are combined so as to cooperatively promote multiplication of beneficial bacteria in the intestinal tracts of sows, reduce the amount of harmful bacteria such as escherichia coli, maintain and regulate the balance of intestinal flora of the sows, increase the number of beneficial bacteria in intestinal contents and excrements, alleviate the action caused by the harmful bacteria, purify the environments in vivo and in vitro, create a good flora environment for new-born piglets and achieve the effects of increasing the weaning weights of the piglets, increasing the weights of the pigs weaned for 3 weeks and reducing the diarrhea occurrence rate. Furthermore, the formula of the feed additive does not contain medicaments, so that drug resistance and drug residues can be avoided.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Method for controlling flow of intestinal contents in a patient's intestines
ActiveUS8696543B2Eliminate the problemAnti-incontinence devicesSurgical needlesLower riskGeneral surgery
There is provided a method for controlling a flow of intestinal contents in the intestinal passageway of a patient's intestines. The method comprises gently constricting (i.e., without substantially hampering the blood circulation in the intestinal tissue wall) at least one portion of the intestinal tissue wall to influence the flow in the intestinal passageway, and stimulating the constricted wall portion to cause contraction of the wall portion to further influence the flow in the intestinal passageway. The method can be used for restricting or stopping the flow in the intestinal passageway, or for actively moving the fluid in the intestinal passageway, with a low risk of injuring the intestines.
Owner:FORSELL PETER
Pure Chinese traditional medicine granular preparation for treating gastroesophageal reflux disease
InactiveCN101138603AGood curative effectNo adverse side effectsHeavy metal active ingredientsDigestive systemSide effectAdditive ingredient
The present invention relates to a pure Chinese medicine granule preparation for treating gastroesophageal reflux disease. The gastroesophageal reflux disease is a common clinical disease, referring to a syndrome caused by the reflux of excessive stomach and duodeno contents into oesophagus. The present invention includes the following ingredients: ruddle, radix bupleuri, Chinese goldthread, evodia rutaecarpa, largehead atractylode, pachyma cocos, hovenia dulcis and zhibanxia, which according to a certain proportion are processed into a pure Chinese medicine granule preparation through certain procedures for treating gastroesophageal reflux disease. The present invention has the advantages of good curative effect, convenience and low price, no adverse side effects.
Owner:祁宏
Compound neomycin sulphate solution and preparation method thereof
InactiveCN101628109AAvoid drug resistanceInhibition of secretionAntibacterial agentsDigestive systemAdditive ingredientGlandular secretion
The invention discloses a compound neomycin sulphate solution and a preparation method thereof. The invention aims to provide a compound neomycin sulphate solution which has obvious effect on treating gastrointestinal infection of birds, treats symptoms and a root and reduces the medicine application frequency and a preparation method thereof. 100 L of solution comprises 2-15 kg of neomycin sulphate, 3-15 kg of colistin sulphate, 0.01-0.05 kg of scopolamine methobromide, 10 kg of alcohol with concentration of 95 percent and the balance of purified water. The invention adopts the neomycin sulphate and the colistin sulphate as antibacterial components, has double effects, can effectively reduce the medicine resistant rate of bacteria and has effect on common Gram negative bacteria causing intestinal infection; and the scopolamine methobromide inhibits glandular secretion, dries intestinal contents and lightens a dehydration symptom so as to have a rapid therapeutic effect.
Owner:TIANJIN SHENGJI GRP CO LTD
Method for separating, screening and identifying earthworm gut culturable antibiotic resistant bacteria
InactiveCN109182127AEasy to controlComprehensiveMicrobiological testing/measurementMicroorganism separationSequence analysisLiquid medium
The invention discloses a method for separating, screening and identifying earthworm gut culturable antibiotic resistant bacteria. The method comprises the following steps: selecting fresh earthwormswhich have obvious clitellum and of which the weights are in a certain range, cleaning the body surface, performing anesthesia, dissecting, and taking intestinal contents; diluting the intestinal contents into a certain concentration gradient with sterile water, coating the diluents of different concentrations on an improved LB solid plate containing antibiotics of different types and contents, and culturing, screening and purifying the bacteria; culturing the purified bacterial colony in an improved LB liquid medium, and then extracting DNA by using a bacterial DNA extraction kit; performing16S rRNA amplification on the extracted DNA, and detecting the amplification product by using gel electrophoresis having the mass fraction of 2%; and performing sequencing analysis on the amplification product, and carrying out comparison on the NCBI after obtaining the sequence.
Owner:NANJING AGRICULTURAL UNIVERSITY
Pretreatment method for extracting metagenome DNA of intestinal contents
ActiveCN107475250AHigh purityPracticalMicrobiological testing/measurementDNA preparationOmnivorePretreatment method
The invention discloses a pretreatment method for extracting metagenome DNA of intestinal contents. The pretreatment method comprises the following steps of: adding a necrotic-tissue removing agent into the intestinal contents to wash for twice, then adding nuclease eliminating solution for washing, cracking cells by adopting a freezing-thawing method and a cracking method, fully releasing DNA in the cells, finally adding protease K, SDS and RNA enzyme to remove protein, fiber and RNA in the cells, and treating products by adopting a conventional method to obtain the metagenome DNA of the intestinal contents. The pretreatment method disclosed by the invention has the advantages that the effect for extracting the DNA of the metagenome DNA of the intestinal contents of omnivorous animals is obvious, and both the extracting purity and the extracting amount can meet downstream experiments such as PCR augmentation and high-throughput sequencing and the like, so that the practicability is very strong.
Owner:广东美格基因科技有限公司
Pressure-reducing collecting device
InactiveCN103768668AEasy dischargeEasily brokenCannulasEnemata/irrigatorsIntestinal ObstructionsEngineering
The invention discloses a pressure-reducing collecting device used in intestinal obstruction operations. The pressure-reducing collecting device comprises a tubular body, the body comprises a first channel, the front end of the body is fixed inside an intestinal tube, the rear end of the body is fixedly connected with a waste containing bag, the body is further provided with a second channel, the first channel is communicated with the second channel, a flushing pipe can be inserted into the second channel selectively, the front end of the flushing pipe sequentially penetrates through the second channel and the first channel to stretch out of the front end of the first channel, and the front end of the flushing pipe is inserted into the obstruction part suffering from intestinal obstruction. The pressure-reducing collecting device mainly has the advantages that when the pressure-reducing collecting device which can be selectively provided with the flushing pipe is in use, the flushing pipe is inserted into the content with obstruction, then irrigation flushing is conducted, the intestinal content with obstruction can be smashed and diluted better, and therefore the content can be discharged out conveniently.
Owner:TOUCHSTONE INTERNATIONAL MEDICAL SCIENCE CO LTD
Quick detection technology for H9 type avian influenza virus
InactiveCN101575649AMicrobiological testing/measurementMaterial analysis by electric/magnetic meansPoultry diseaseIntestinal structure
The invention discloses a method for detecting virus from a poultry disease material, and application of the method. The method comprises the following steps: sterilely acquiring tissues of detected dead poultry such as intestinal contents, lung, air chamber, intestines, spleen, liver or heart, or sterilely acquiring blood, trachea swab, cloacae swab or excrement of detected living poultry; extracting RNA from a sample by adopting a Trizol method or a Mini BEST virus RNA extraction kit; using the extracted RNA as a template to carry out an RT-LAMP reaction with upstream and downstream primers at the outside and upstream and downstream primers at the inside, sampling the reactant after the RT-LAMP reaction is finished to carry out agarose gel electrophoresis detection, and judging whether the detected poultry are infected by H9 type avian influenza virus by a way that whether a ladder type band with RT-LAMP reaction specificity appears.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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