42 results about "American cockroach" patented technology

The invention discloses nitrogen-doped carbon quantum dots derived from American cockroach dregs as well as a preparation method and application of the nitrogen-doped carbon quantum dots, and belongs to the technical field of nitrogen-doped carbon quantum dots. The nitrogen-doped carbon quantum dots are prepared from the American cockroach dregs by a one-step hydrothermal method, optimal synthesis parameters are obtained through experimental optimization of synthesis conditions, and the prepared nitrogen-doped carbon quantum dots have excellent optical characteristics and stability; the invention also provides an application of the nitrogen-doped carbon quantum dot in Ag < + > detection, the nitrogen-doped carbon quantum dot has good selectivity, sensitivity and anti-interference performance on Ag < + >, a detection mechanism is an internal filtering effect and light-induced electron transfer, and the nitrogen-doped carbon quantum dot can be used as a preferred fluorescent material of an Ag < + > fluorescent sensor. The nitrogen-doped carbon quantum dots synthesized by the method not only have an Ag < + > sensing function, but also realize high-valued utilization of medicine residues, and also provide a new thought for self-doping of heteroatoms.

The invention belongs to the technical field of quality detection of traditional Chinese medicinal materials, and particularly relates to a method for determining the content of amino acid in periplaneta americana. The method comprises the following steps: a, preparation of a test solution: taking American cockroach medicinal material powder, adding water, carrying out ultrasonic treatment, carrying out solid-liquid separation to obtain a separation solution, enabling the separation solution to pass through a cation exchange resin column, soaking, eluting, collecting the eluent, drying by distillation, adding a hydrochloric acid solution, dissolving, filtering, and taking a subsequent filtrate to obtain the test solution; b, preparing a reference substance solution; and c, determination: respectively and precisely sucking the reference substance solution and the test solution, injecting into a high performance liquid chromatograph, determining, and calculating the content by using an external standard two-point method logarithmic equation. The method provided by the invention has good linearity, repeatability, stability and recovery rate, and can effectively monitor the content of various amino acids in periplaneta americana.

The invention relates to the technical field of pharmaceutical preparations, in particular to oral in-situ gel and a preparation method and application thereof.The in-situ gel is prepared from, by weight, 0.09-2.5 parts of American cockroach extract, 0.3-3 parts of gel matrix, 86.7-99.4 parts of water and a pH regulator, the in-situ gel is formed by a periplaneta americana extract, a gel matrix and water according to a specific ratio in the presence of a pH regulator, the gel is a clear pale yellow to yellow solution at room temperature, the gel is formed in gastric juice after oral administration as a solution, the gel can be retained in the stomach, more importantly, the burst release of the medicine can be remarkably reduced, and the curative effect of the medicine is improved. The medicine dissolution is improved, and the medicine is ensured to be continuously and slowly released to play a role in treating the peptic ulcer.

The invention relates to an intestinal flora metabolite extract in periplaneta americana excrement and a preparation method and application thereof, and relates to the technical field of coprophilous fungi, and the preparation method comprises the following steps: S1, collecting excrement, pretreating, and preserving the excrement by using an excrement preserving fluid; s2, putting the stored periplaneta americana excrement suspension into a freeze dryer, performing vacuum drying, crushing and sieving, and then performing ethanol reflux extraction; S3, performing reduced pressure concentration on filtrate at 70 DEG C to obtain extract, recovering ethanol, and stirring to prepare dispersion liquid; s4, sequentially extracting the dispersion liquid by using petroleum ether, ethyl acetate and n-butyl alcohol to obtain a powdery metabolite extract; integrity and metabolic activity of intestinal flora preservation are improved through excrement preservation liquid, and then an extract with a high anti-inflammatory effect is obtained through extraction.

The utility model belongs to the technical field of periplaneta americana breeding equipment, and particularly relates to an escape-proof device for breeding periplaneta americana, which comprises a breeding box, a breeding net bin is arranged in the breeding box, two seat holes are formed in the left side of the breeding box, a collecting bin is arranged on the inner wall of the bottom of the breeding box, and limiting mechanisms are arranged among the breeding net bin, the collecting bin and the seat holes. A connecting plate is arranged on the right side of the feeding net bin, a first through hole and a second through hole are formed in the right side of the feeding box, the first through hole is located above the second through hole, the connecting plate is matched with the first through hole, and the collecting bin is matched with the second through hole. The periplaneta americana feeding device is reasonable in design, the aim of conveniently feeding periplaneta americana can be achieved by arranging the feeding mechanism, the aim of preventing the periplaneta americana from escaping and being difficult to control during feeding can be achieved, and the aim of conveniently collecting mature periplaneta americana can be achieved by arranging the reset mechanism.

The invention discloses a composite solution for treating reflux esophagitis and a preparation method and application thereof, and relates to the technical field of pharmaceutical preparations, the composite solution is formed by physically mixing drug-loaded microspheres and a gastric buoyant raft matrix solution; wherein the drug-loaded microspheres are solid particles which take thiolated pectin as a carrier, are crosslinked and cured through divalent metal ions and are coated with a periplaneta americana extract; the gastric buoyant raft matrix solution comprises a hydrophilic colloid, a component capable of generating gas when encountering acid and a stabilizer. The magnesium mercapto pectin microspheres provide excellent mucous membrane adhesion, and the acting time of the medicine on the esophageal focus is prolonged; the gastric buoyant raft substrate forms a physical barrier in a gastric acid environment to inhibit regurgitation of gastric contents. Through cooperation of the two, a dual treatment mechanism of local repair and source control is realized. The preparation can remarkably prolong the in-vivo residence time and effectively promote esophageal mucosa healing, and the curative effect is better than that of a pure periplaneta americana extract preparation.

The invention relates to the technical field of grass carp feed, and particularly discloses functional feed based on American cockroach chitin as well as a preparation method and application of the functional feed. The functional feed based on the American cockroach chitin is a grass carp compound feed. Comprising 2.5-20% by weight of American cockroach chitin. The periplaneta americana chitin is added into the grass carp compound feed, so that digestion, absorption and utilization of nutrient substances by juvenile grass carps can be remarkably improved, growth of the juvenile grass carps can be promoted, the disease resistance of the juvenile grass carps can be enhanced, and the conversion rate of the feed can be increased. After a proper amount of the feed is added, the feed does not generate adverse effects on various physiological functions of the grass carp, the formula cost is effectively reduced, and the feed has relatively high cost performance. By using the additive, the culture benefit of the grass carp can be remarkably improved. The problem that in the prior art, no functional feed is prepared from American cockroach chitin as a feed additive is solved.

The invention belongs to the technical field of breeding, and provides a breeding method of periplaneta americana. The breeding method of periplaneta americana comprises the following steps: (1) feeding young nymphs: feeding newly hatched nymphs in a breeding box for 30-40 days by adopting first feed to obtain young nymphs; the first feed comprises the following components in parts by weight: 30-36 parts of basic grains, 4-5 parts of composite acid-base materials and 1.2-1.8 parts of composite Chinese herbal medicines; (2) feeding the young nymphs: continuously feeding the young nymphs in the breeding box for 3-5 months by adopting second feed until adult nymphs are obtained; the second feed comprises the following components in parts by weight: 30-36 parts of basic grains, 2-3 parts of composite acid-base materials and 4-6 parts of composite Chinese herbal medicines. According to the breeding method, the periplaneta americana nymphs are bred under different conditions in stages, and a new feed formula is adopted, so that the requirements of the periplaneta americana nymphs in different growth stages are met, the digestion and absorption functions of the periplaneta americana nymphs in different growth stages are improved, the breeding cycle is shortened, and the survival rate, breeding efficiency and product medicinal quality of periplaneta americana are improved.

This invention discloses a composition and its preparation method for treating human papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN), belonging to the field of traditional Chinese medicine technology. The formula consists of 6-15 parts of American cockroach, 5-11 parts of hawthorn, 5-11 parts of chicory, 3-9 parts of *Scutellaria baicalensis*, 8-20 parts of *Sophora flavescens*, and 6-15 parts of *Cnidium monnieri*, and also contains 0.01-1 parts by weight of toad venom. The dosage form is one or more of the following: external gel, rinse, foam, suppository, and film preparation. It has high safety and is suitable for HPV infection and CIN changes, solving the problem of poor efficacy in existing treatments.

The invention discloses a method for crude extraction of periplaneta americana breeding waste and application of the periplaneta americana breeding waste, and belongs to the technical field of periplaneta americana breeding waste extraction. The method for coarsely extracting the American cockroach breeding waste comprises the following steps: (1) grinding the American cockroach breeding waste to obtain American cockroach breeding waste powder, weighing the American cockroach breeding waste powder, and putting the American cockroach breeding waste powder into a container; and (2) preparing a leaching solution, namely adding the American cockroach breeding waste powder into absolute ethyl alcohol, fully and uniformly mixing, sealing by using a preservative film, leaching and filtering at room temperature to obtain the leaching solution, and storing in a refrigerator. The American cockroach breeding waste excrement, shell and ootheca crude extract have different degrees of bacteriostatic activity on fusarium verticillium and alternaria alternata; the method has the advantages of small environmental pollution, difficult generation of drug resistance and the like, and can avoid the problems caused by the use of chemical bactericides; a new thought is provided for innocent treatment and recycling of the American cockroach breeding waste.

The invention discloses a functional beverage produced by compounding insects and plants. The functional beverage is prepared from insect raw materials, plant raw materials and a solvent, the insect raw material is periplaneta americana, the plant raw material is sweet-scented osmanthus, and the solvent is distilled liquor; the material ratio of the periplaneta americana to the sweet-scented osmanthus is 1-2, and the material-liquid ratio of the total mass of the periplaneta americana and the sweet-scented osmanthus to the distilled liquor is 1: (15-30) g / mL. The functional beverage is prepared by scientific compatibility of periplaneta americana and sweet-scented osmanthus.

The utility model belongs to the field of periplaneta americana breeding, particularly relates to a cleaning and drying device for periplaneta americana breeding, and aims to solve the problems that when an existing cleaning and drying device for periplaneta americana breeding is used, periplaneta americana in a porous iron box can be turned only by manual operation of a worker, time and labor are wasted, and working efficiency is high. And when periplaneta americana is taken out from the cleaning liquid, cleaning liquid is left on a porous iron box and periplaneta americana, the drying time is prolonged, and the efficiency is low, so that the following scheme is provided: the periplaneta americana cleaning device comprises a shell, a driving mechanism, a drying mechanism and a porous box. According to the periplaneta americana cleaning and drying device, periplaneta americana can be automatically cleaned and dried through cooperation of all the components, the processing time of the periplaneta americana is shortened, the structure is simple, operation is easy, and the processing efficiency and practicability are improved.

The present application relates to the technical field of medicine, and in particular to Periplaneta americana L. extract, a preparation thereof, a preparation method and application thereof. The method for preparing Periplaneta americana L. extract includes the steps: 1) soaking fresh Periplaneta americana L. extract with ethanol; 2) reflux extracting with ethanol, and then filtering and combining the filtrates; and 3) concentrating the filtrate into extractum. The method uses environmental protection reagents such as ethanol, increases the content of bioactive substances of Periplaneta americana L. extract, saves time, human and material resources, saves energy and reduces production cost. The Periplaneta americana L. extract obtained by the present method has better anti-inflammatory activity and higher content of amino acids.

The invention relates to the technical field of biological medicine, and discloses a preparation method for improving wound scar repair effect, which comprises the following steps: crushing dried periplaneta americana, and soaking with ethanol; then carrying out reflux extraction, filtration, concentration and drying to obtain a dried periplaneta americana insect extract; dissolving collagen and hyaluronic acid in glycerol, adding the dried periplaneta americana insect extract, uniformly stirring, and preparing a medicine storage layer; wherein the collagen accounts for 5% of the total amount, the hyaluronic acid accounts for 1% of the total amount, the glycerol accounts for 89% of the total amount, and the American cockroach dried insect extract accounts for 5% of the total amount. The medicine storage layer is arranged on the backing layer in a stepping mode and covered with the anti-sticking layer, so that the scar patch capable of improving the wound scar repairing effect is manufactured. According to the preparation method for improving the wound scar repair effect, wound healing is promoted, scar hyperplasia is inhibited, the anti-inflammatory effect can be achieved, the moisturizing effect is good, natural components are adopted, safety is high, and the scar repair effect is improved.

The invention provides a periplaneta americana glucan Pa1-B1 as well as a preparation method and application of the periplaneta americana glucan Pa1-B1. Belongs to the technical field of biological medicine. The American cockroach polysaccharide Pa1-B1 is glucan of which the main chain is connected by alpha (1-> 4), glucose on a side chain is connected to the main chain by alpha (1-> 6), and the weight-average molecular weight is 36000-42000 kDa. The periplaneta americana glucan Pa1-B1 is obtained by the following steps: crushing a periplaneta americana scorpion, degreasing with 95% ethanol, carrying out enzyme hydrolysis extraction under an alkaline condition, and separating and purifying through alcohol precipitation, ion exchange column chromatography and gel column chromatography. The American cockroach glucan Pa1-B1 disclosed by the invention is a polysaccharide which is found in American cockroaches for the first time and is novel in structure, and has the activity of enhancing immune regulation. After in-vivo advanced administration, periplaneta americana glucan Pa1-B1 can powerfully train body immunity, significantly inhibit malignant tumor metastasis and prolong the lifetime of tumor-bearing mice, and has an anti-tumor effect for a long time after training and immunization. Therefore, the American cockroach glucan Pa1-B1 disclosed by the invention can be used for preparing anti-tumor or anti-tumor metastasis medicines for enhancing the immune function and training immunity.

The invention belongs to the technical field of biomedicine, and particularly relates to application of periplaneta americana polypeptide and periplaneta americana peptide B serving as an immunologic adjuvant. The amino acid sequence of the periplaneta americana polypeptide and periplaneta americana peptide B is shown as SEQ ID NO.1. According to the invention, influenza virus H1N1RBD protein is combined with periplaneta americana peptide B, mice can be induced to generate an anti-RBD protein specific IgG antibody with higher titer, and the titer of the antibody can be increased along with the increase of immunization times. Meanwhile, the level of T subsets of lymphocytes of a mouse body can be changed, so that the T subsets can resist pathogen invasion to a certain extent. The periplaneta americana polypeptide periplaneta americana peptide B provided by the invention can be conveniently obtained through mass synthesis, can be used as a novel micromolecular immunologic adjuvant, and is beneficial to enhancing the specific immune response of an organism.

The application provides an American cockroach glucan Pa1-B1 and a preparation method and application thereof, and belongs to the technical field of biological medicines. The American cockroach glucan Pa1-B1 is a glucan with an alpha (1→4) linked main chain, and glucose on the side chain is alpha (1→6) connected to the main chain, and the weight average molecular weight is 36000 kDa to 42000 kDa. The American cockroach glucan Pa1-B1 is obtained by crushing whole American cockroaches, defatting with 95% ethanol, extracting under alkaline conditions by enzymatic hydrolysis, and then alcohol precipitation, ion exchange column chromatography and gel column chromatography separation and purification. The American cockroach glucan Pa1-B1 is a novel polysaccharide first discovered in American cockroaches, has the activity of enhancing immune regulation, and can effectively train the body immunity, significantly inhibit malignant tumor metastasis, prolong the survival period of tumor-bearing mice, and has long-term anti-tumor effect after training immunity. Therefore, the American cockroach glucan Pa1-B1 can be used for preparing an immune function enhancing, training immunity anti-tumor or anti-tumor metastasis drug.

The invention provides a zinc ion chelating modified peptide derived from the traditional Chinese medicine American cockroach and its application. The sequence of the zinc ion chelating modified peptide is: GHCSPPFAPRL, the three amino acids GHC from the 1st to the 3rd position have a coordination chelating effect on divalent metal zinc ions, and the eight amino acids SPPFAPRL from the 4th to the 11th position are natural polypeptide sequences derived from American cockroach. The metal zinc ion chelating modified peptide adopts a polypeptide solid phase synthesis technology to prepare a linear polypeptide GHCSPPFAPRL, and after purification, a microwave synthesis method is used to chelate the metal zinc ions with the three amino acid residues of GHC; and then an ethanol precipitation method is used to precipitate it from the reaction system. The zinc ion chelating modified peptide has the functions of anti-oxidation, promoting cell migration and / or proliferation, promoting wound healing, etc., and also has low cytotoxicity to red blood cells, and the preparation process is simple and easy to be industrialized on a large scale.

The invention discloses a preparation method and application of American cockroach ground powder, and belongs to the technical field of medicinal material processing. After being degreased, the periplaneta americana medicinal material is crushed and sieved to obtain the periplaneta americana ground powder. The content of chitin in the periplaneta americana raw medicinal material is about 15-20%, the content of chitin in the periplaneta americana powder after partial chitin is removed by a physical method is less than or equal to 6%, the removal rate of chitin is greater than 50%, the yield of the powder is greater than 70%, and the problem of stomach fullness and discomfort of a patient after the patient takes the periplaneta americana powder is solved. The content of effective components in the American cockroach ground powder and the adaptability of patients are improved, and the effects of reducing toxicity and enhancing efficiency of traditional Chinese medicine processing are achieved.

This invention belongs to the field of pharmaceutical technology, specifically relating to the preparation of liposomes based on American cockroach oil and their application as delivery carriers for American cockroach extracts. This invention is the first to introduce active oils from American cockroaches as a functional membrane component into a liposome construction system, successfully achieving partial substitution of traditional cholesterol and reducing the amount of cholesterol used in traditional liposomes. Simultaneously, the introduction of active oils may endow liposomes with additional biological functions, such as anti-inflammatory and repair-promoting effects, while also increasing the stability of the liposomes. This lays the foundation for the subsequent development of multifunctional carriers with synergistic therapeutic effects.

The invention relates to the technical field of periplaneta americana powder microscopic flaking, and discloses a periplaneta americana powder microscopic identification method which comprises the following steps: S1, sample degreasing: dividing a periplaneta americana adult into five parts, namely a head part, a chest back plate, wings, an abdomen part and chest feet by using tweezers, respectively grinding into powder, taking 100g of powder of each part of the periplaneta americana, and uniformly mixing the powder with the powder of each part of the periplaneta americana; respectively filling the materials into a filter paper tube, putting the filter paper tube into an extraction tube of a Soxhlet extractor, soaking the American cockroach powder with an extracting solution, and removing grease; s2, sample tabletting: taking the degreased powder, respectively adding distilled water, chloral hydrate and dilute glycerol for tabletting, and preparing a water-loaded tablet, a chloral hydrate cold-loaded tablet and a chloral hydrate permeabilized tablet; s3, microscopic observation: placing the prepared loading sheet under an optical microscope, and carrying out image acquisition and analysis on powder characteristics by using image processing software equipped for the microscope; and S4, identification standard. Compared with the prior art, the method has the advantages that grease is convenient to remove, the observation definition and accuracy are improved, a microscopic identification standard is established, and an identification system is perfected.

The invention belongs to the technical field of aquaculture, pet breeding and animal protection, and discloses an atail frog anesthesia ointment, a preparation method and application, every 100 g of the atail frog anesthesia ointment is prepared from the following components by weight: 50-80 g of vaseline, 5-10 g of beeswax, 5-10 g of a periplaneta americana extract, 1-5 ml of eugenol and sterile plaster cloth. According to the preparation method of the anesthetic ointment, the anesthetic efficiency is improved, and the anesthetic risk is reduced. And the blank of the antail frog anesthetic ointment in the market is filled. Effective animal welfare is provided for surgery, treatment, collection and protection of the frogs without tail eyes in the future.

The present invention provides a polyarginine modified peptide derived from the traditional Chinese medicine American cockroach and its application. The nucleotide sequence of the polyarginine modified peptide derived from the traditional Chinese medicine American cockroach is shown in SEQ ID NO.1. The modified peptide can be prepared by using a polypeptide solid phase synthesis technology, and has the characteristics and advantages of mature preparation technology, high yield, good purity, strong stability, etc. At the same time, it has a wide antibacterial spectrum and has good antibacterial (including Gram-positive cocci and Gram-negative bacilli) and antifungal (Candida fungi) activities. MTT experiments and hemolytic activity studies show that it also has low cytotoxicity and has good application prospects and development value.

The utility model discloses a periplaneta americana breeding device which is provided with an oxygen concentration sensor, the oxygen concentration sensor is in signal connection with a controller, an oxygen concentration signal in a box body is transmitted to the controller, and the controller controls a temperature control ventilation assembly to adjust the oxygen concentration in the box body. The temperature control ventilation assembly is provided with a fresh air valve, an air inlet fan, an air return valve and an air exhaust fan, and fresh air with high oxygen concentration enters the box body through an air inlet pipe by closing the air return valve and opening the fresh air valve and the air inlet fan; and the exhaust fan is started, so that the air with lower oxygen concentration in the box body is discharged out of the box body through the exhaust pipe, and external circulation of the air is realized. When the oxygen concentration sensor detects that the oxygen concentration in the box body meets the requirement, the fresh air valve and the air exhaust fan are closed, the air return valve is opened, the air exhaust pipe is communicated with the air inlet fan, air is fed into the box body again through the air inlet pipe from the air exhaust pipe, air internal circulation is formed, and heat loss in the box body is reduced.

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a pharmaceutical composition for treating oral inflammation and a preparation method and application thereof. The invention provides a pharmaceutical composition for treating oral inflammation. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 30-70 parts of American cockroach, 30-50 parts of emblic leafflower fruit, 20-30 parts of dragon's blood, 20-30 parts of dandelion, 10-20 parts of red paeony root and 2-8 parts of liquorice. The composition disclosed by the invention has a good treatment effect on oral inflammation, especially has a remarkable treatment effect on oral ulcer, and can obviously relieve the injury degree of an oral ulcer wound.

This invention discloses the application of methyl N-hydroxybenzoxime acid in the preparation of cockroach attractants, and also provides a composition for attracting American cockroaches, comprising an effective amount of methyl N-hydroxybenzoxime acid as an attractant, wherein the mass percentage of methyl N-hydroxybenzoxime acid is 0.01% to 10%. The composition may further include auxiliary attractants selected from 3-hydroxy-2-butanone, butyric acid, isovaleric acid, oleic acid, linoleic acid, nonanal, decanal, hexadecanoic acid, and stearic acid, or mixtures thereof. The composition also contains an attractant-acceptable carrier, such as at least one of food substrates, solvents, and solid inert materials, and can be formulated into liquid, gel, granule, or aerosol formulations for monitoring American cockroach population dynamics and / or trapping American cockroaches. This attractant component is derived from the insect itself, exhibiting good biocompatibility and environmental friendliness, providing a new technical means for the green control of American cockroaches.

This invention discloses a modified American cockroach polypeptide derivative, its preparation method, polypeptide composition, and applications. The amino acid sequence of the polypeptide derivative is shown in SEQ ID NO: 1 (PA1702); it is modified by replacing the asparagine at position 13 of the natural polypeptide PA1701 with glutamine, and can be prepared by Fmoc solid-phase polypeptide synthesis. Network pharmacology predicts that it acts on 59 core anti-inflammatory targets, enriched in pathways such as PI3K-Akt and MAPK. Molecular docking shows that PA1702 has a strong binding affinity for TNF-α and AKT1. In vitro experiments confirm that PA1702 is non-cytotoxic, can inhibit LPS-induced release of NO, TNF-α, IL-6, and IL-1β, and promote cell migration. The above-mentioned polypeptide derivative can be used as an active ingredient in cosmetics or pharmaceutical compositions; it has anti-inflammatory, skin barrier repair, and wound healing promotion effects, and can be used to prepare anti-inflammatory, skin barrier repair, and wound healing-promoting cosmetics or drugs.

The invention provides a powerful hemostatic adhesive prepared by taking American cockroach dregs as a main raw material. The preparation method comprises the following steps: firstly, extracting and preparing chitin whiskers from waste American cockroach dregs, taking the chitin whiskers as a polymer matrix of the adhesive, taking an aqueous solution of a deep eutectic solvent as a system medium, taking thrombin as a hemostatic functional substance, and improving the overall mechanical properties by gelatin, thereby finally obtaining the biological adhesive with good adhesion and hemostatic performance. According to the product, under the condition that a cross-linking agent does not need to be used, through heating-cooling operation, a polymer skeleton network is constructed through interaction between chitin whiskers and gelatin, a channel is provided for transmitting moisture and nutrient substances to damaged tissues, and the product has degradability while achieving an ideal adhesion effect; the whole system effectively fuses the components, is friendly and easy to prepare, and integrates respective advantages. In addition, the activity and the stability of the thrombin in the eutectic solvent are both in ideal states, so that the adhesive can play a powerful hemostatic role.

The invention discloses the technical field of biology, and particularly relates to a CYP9E22 gene and application of the CYP9E22 gene in regulation and control of resistance of periplaneta americana to cyhalothrin, and the nucleotide sequence of the CYP9E22 gene is shown as SEQ ID NO.1.

The invention provides the method for identifying the polypeptide and the protein in the active extract CII-3 of the periplaneta americana, the components such as the polypeptide and the protein can be analyzed through a nano-liter liquid phase and high-resolution mass spectrum tandem technology, and the identification work of corresponding macromolecular compounds is completed in combination with database matching. The invention fills the blank of CII-3 macromolecular substance research, enriches the material basis of CII-3, and provides macromolecular compound information for further development of related researches such as pharmacodynamic material basis, action target and the like of periplaneta americana series products taking CII-3 as a raw material.