93 results about "Defatting" patented technology

This invention relates to preparations of collagen proteins and collagen peptides. It comprises: 1) mechanically pulverizing alkali leather or raw leather 100, washing at atmosphere and regulating pH 7- 10; 2) adding degreasing enzyme 0.1 - 1.2, stirring for 0.5 - 4 hours at 20 - 45 deg.C, washing; 3) adding surfactant 0.1 - 1.2, stirring for 0.5 - 4 hours at 20 -45 deg.C, washing; 4) adding active biological enzyme 0.1 - 0.8 at 30 - 65 deg.C, degrading for 0.5 - 12 hours; 5) heating to 75 - 90 deg.C for 15 - 30 minutes to inactivating enzyme; 6) filtering and distilling at 40 - 65 deg.C with pressure reduction; and 7) spraying to dry and obtain solid collagen proteins and collagen peptides powders. Owing to alkali degreasing enzyme and surfactant, leather degreasing rate will be up to 95% to produce easily soluble products with low cost and high productivity. The products can be used in skin nourishment and healthy food.

The invention discloses blended non-woven fabric and a preparation technology thereof. The blended non-woven fabric comprises the preparation raw materials of degummed silk protein fiber and degummed natural vegetable fiber, wherein the mass ratio of the degummed silk protein fiber to the degummed natural vegetable fiber is (20:80) to (80:20). The blended non-woven fabric not only has the advantages of softness, skin protection and skin favorableness of silk, but also has the elasticity and water absorption of fiber. The blended non-woven fabric is simple in preparation technology, and can be widely applied to industrials of facial masks, sanitary towels, sanitary pads, paper diapers, cosmetics, respirators, hygienic products and medical supplies.

The invention discloses a fish skin collagen support loading epidermal growth factors and a preparation method thereof. The fish skin collagen support is composed of cross-linked fish skin collagen support bodies and nanometer particles of the load epidermal growth factors. The preparation method comprises the steps that after dewatering, degreasing, enzyme treatment and dialysis is conducted on fish skin, the cross-linked fish skin collagen support bodies are prepared, then the cross-linked fish skin collagen support bodies are soaked in a solution of the nanometer particles of the load epidermal growth factors, and the fish skin collagen support loading the epidermal growth factors is obtained after freezing and drying. The support has a slow releasing effect on the loaded factors, and prolongs the acting time of the epidermal growth factors at a trauma position. The fish skin collagen support obtained through the method has the advantages of being good in biocompatibility and biodegradability, free of immunogenicity and the like, is harmless to a wound surface, can effectively induce wound surface healing and is a good medical material.

The invention discloses a method for preparing donkey skin collagens. In the method, fresh donkey skin is used as a raw material, and modified donkey skin collagen products are modified by steps of unhairing, degreasing, crushing, alkali solution soaking, low-temperature enzymolysis, purification, modification and the like. The donkey skin collagen products prepared by the method is characterized by retaining a triple helical structure, possessing biological activity, being homologous and compatible with human organisms and skin, along with high biological degradability, low antigenicity and the like; and the donkey skin collagens can be diluted by water in any ratio and be used for preparing surgical implants and tissue engineering medical products and developing medicaments, medical preparations, healthcare products and the like.

The invention provides a vaseline oil gauze producing method. The method includes preparing a medical absorbent gauze, vaseline and paraffin oil as raw materials; placing the vaseline and the paraffin oil in proportion into a stainless steel container or a ceramic container to heat and melt, and stirring evenly; sinking the medical absorbent gauze into the heated, melted, diluted and mixed oil; placing the container accommodating the vaseline oil gauze into a dry heat sterilizer to sterilize. The volume proportion of the vaseline and the paraffin oil is 20:1, and weight proportion of the vaseline and the gauze is 2:1. The vaseline oil gauze produced by the method has the advantages of lubricating, anti-adhesion, anti-oxidation and prompting cell growth and wound healing, can be used for covering surgical wounds and wound surfaces, draining wounds and covering normal skin, and plays a role of moisturizing and caring skin, anti-aging for skin cells and the like.

Biogenous artificial bone tissue is set inside the solution of serial surfactants for full wetting and penetration, the solution enters to the bone tissue and the fat tissue falls down to disperse inthe surfactant solution and to be eliminated via water rinsing. The surfactant used may be biodegraded inside body and is non-toxic and harmless. The said process has high defatting effect and efficient antigenicity reducing effect. The processed artificial bone has even high mechanical performance, the production process has no pollution, and the product has no residual toxic matter and is convenient in storing and transportation.

The invention discloses an energy-saving and environment-friendly mink skin tanning method, which comprises the following working procedures of: A) carrying out a soaking working procedure in soaking solution with the solution formula of 25g/L of table salt and 1g/L of soaking enzyme100-c; B) carrying out a degreasing and re-soaking working procedure in degreasing and re-soaking solution with the solution formula of 1ml/L of degreasing agent (Supralan80), 1ml/L of wetter ethanoic acid (HAC) and 0.5ml/L of degreasing enzyme200; C) carrying out a softening and picking working procedure in softening and picking solution with the solution formula of 40g/L of table salt, 2-3ml/L of Super lotan A, 0.5ml/L of Sinxit and 6g/L of glycolic acid; and D) carrying out a tanning working procedure in tanning solution with the solution formula of 40g/L of table salt, 10g/L of aluminite powder (Novaltan Al) and 4-5g/L of Tanning assist B. The energy-saving and environment-friendly mink skin tanning method disclosed by the invention has the advantages of simpleness in manufacturing and processing the mink skin, small water consumption and pollution, and higher energy saving and environment protection effect.

The invention discloses a weakly-acidic high transparent soap which is mainly prepared from amino acid surfactant, humectant, neutralizer and deionized water, wherein the amino acid surfactant comprises glutamic acid surfactant, glycine surfactant and sarcosine surfactant; and based on the total mass of the weakly-acidic high transparent soap, the mass percent of the glutamic acid surfactant is 25-30%, the mass percent of the glycine surfactant is 8-10%, and the mass percent of the sarcosine surfactant is 5-8%. The invention also discloses a preparation method of the transparent soap. The pH value of the weakly-acidic high transparent soap is close to that of the skin; and the weakly-acidic high transparent soap has moderate degreasing power, does not hurt the skin, is easy to foam and can produce abundant foam.

The invention discloses a preparation method of an alginate dressing for stopping bleeding. The preparation method comprises the following steps of placing alginate and pectin into a stirrer according to the part weight, and heating to obtain a mixture I; mixing diatomite, sodium pyrophosphate and sodium polyacrylate, then, heating, preserving heat, cooling and sieving to obtain a mixture II; adding the mixture II into the mixture I, then, adding sorbitol, hyaluronic acid, aminohexanoic acid and linoleic acid, and regulating the pH value after uniformly stirring at room temperature to obtain a mixture III; and soaking absorbent gauze into the mixture III, drying, sealing, packaging and irradiating to obtain the alginate dressing. The dressing prepared by using the preparation method not only has a bacterium inhibiting effect, but also can achieve a bleeding stopping effect so as to be widely applied to clinical treatment.

The invention discloses a composite facial mask nutrient solution. The solution is prepared from, by weight, 8-20 parts of tomatoes, 12-30 parts of cucumbers, 4-10 parts of bitter gourds, 4-8 parts of lemons, 3-6 parts of vitamin A, 2-4 parts of vitamin E, 4-8 parts of olive oil, 0.5-2 parts of hyaluronic acid, 0.5-2 parts of water-soluble collagen, 1-3 parts of pearl powder, 5-10 parts of skim milk, 0.8-1.5 parts of honey, 0.02-0.06 part of ethyl alcohol, 0.05-0.15 parts of sodium citrate and 20-50 parts of deionized water. The provided composite facial mask nutrient solution has no additive, and has no stimulation on the skin, has good functions of detoxifying and beautifying as well as whitening and moisturizing, can effectively clean and lubricate the skin, has a good function of maintaining facial and cervical skin and good effects of removing freckles and wrinkles, has no toxic and side effects, and is worth popularizing.

The invention belongs to the technical field of biological materials of tissue engineering and specifically relates to an artificial skin prepared by collagen and a polymer material through ultrasonic treatment. The preparation method of the artificial skin comprises the following steps of performing washing, cutting, sterilization treatment, degreasing treatment, tissue mashing treatment, acidic enzymolysis, salting-out and dialysis treatment, defoaming treatment, crosslinking treatment, treatment by a modifier, ultrasonic treatment, freeze-drying and vacuum drying on a tendon or dermis tissue. The artificial skin provided by the invention can not only protect a wound as a temporary wound cover for a human body, but also repair the wound by permanently retaining growth of a dermal scaffold, thereby overcoming the shortcomings of expensive price, limited sources, long manufacturing time, short preservation time, injuries to an organism caused by secondary skin grafting and the like of an existing artificial skin product. The product prepared by the preparation method provided by the invention has the advantages of extensive materials, short production cycle, long preservation time and low price, and is suitable for clinical extensive application.

The invention discloses a preparation method of non-denatured collagen from yak skin. The preparation method comprises the following steps: (1) pretreatment; (2) degreasing and impurity removal; (3) acidification and homogenization; (4) enzymolysis; (5) salting-out purification: adding saturated saline to supernatant obtained from step (4), continuously performing stirring until no white flocculesare continuously separated out, and then conducting standing and centrifuging and collecting precipitates; (6) isoelectric point purification: dissolving the precipitates obtained in step (5) with anacetic acid solution, then adjusting the pH of the solution to 6.5-7.0 by using NaOH, conducting standing and centrifuging, and collecting precipitates; (7) desalting; and (8) drying. The method of the present invention overcomes the disadvantages of damage on the triple helix structure of collagen caused by traditional collagen preparation processes. By adopting low-temperature preparation in entire process, the acetic acid, enzymatic hydrolysis, freeze drying and other processes are used to obtain the non-denatured collagen from yak skin. The process of the present invention is simple in operations and capable of achieving large-scale industrial production. The prepared non-denatured collagen from yak skin has good development and utilization prospects in the biomedical fields such as biological dressings and drug delivery carriers.

The invention discloses a method for preparing fishskin source acellular dermal matrix. The method comprises the following steps of (1) fishskin pretreatment; (2) sterilization and disinfection treatment; (3) ungreasing treatment; (4) decoloring treatment; (5) high and low permeability treatment; (6) strong base and ablation treatment, (7) multigelation treatment; and (8) freeze-drying and sizingtreatment. According to the method, osmotic pressure is used to alternatively process lytic cells, at the same time, the combination of strong base ablation and multigelation methods removes cellularconstituents to achieve a more ideal effect of removing cells, the prepared fishskin source acellular dermal matrix has a double-deck structure, the treatment process has little damage on a dermal supporting structure, the integrity of a timbering material can be saved well, and the adhesion and growth of the cells in a later stage is facilitated.

The invention relates to a collagen multifunctional absorbable medical suture line and a preparation method thereof. The preparation method of the collagen multifunctional absorbable medical suture line comprises the following steps of: (1) compound lipase degreasing; (2) ultrasonic enhanced degreasing; (3) compound catalase decoloration; (4) ultrasonic enhanced decolorization; (5) nano and biological function composite tanning; and (6) drying and sterilization. The collagen multifunctional absorbable medical suture line prepared with the method has the advantages of being high in tensile strength, controllable in absorption time, firm to knot, good in biocompatibility, free of adverse reactions in the human body and lasting in antibacterial performance. According to the preparation method, degreasing and decolorization are performed by using a composite biological enzyme process and an ultrasonic enhanced process, so that the method is environment-friendly and high in degreasing and decolorization efficiency; the tensile strength of the collagen medical suture line is greatly enhanced through nano and biological function composite tanning; the absorption time of the suture line inthe body is controllable; and the collagen medical suture line has the lasting antibacterial performance.

The invention relates to the technical field of food processing, and discloses a method for extracting collagen in a fish skin by low-dose irradiation. Main processes comprise the following steps of making the fish skin into fish-skin minced meat, soaking the fish-skin minced meat in a fishiness removing solution, then making the fish-skin minced meat into fish-skin material liquid, afterwards, adding glyceride hydrolase, a flavor enzyme and a compound catabolic enzyme into the fish-skin material liquid, putting an obtained mixture in a cobalt-60 irradiation field to carry out irradiation, making the irradiated liquid material into a concentrated solution through heating inactivation, filtration, discoloration and concentration, and subjecting the concentrated solution to spray drying to make the collagen. According to the method for extracting the collagen by the low-dose irradiation, which is provided by the invention, through applying the irradiation to the material liquid in enzymolysis and degreasing processes, the enzymolysis rate can be improved; the hydrolyzation time is decreased; the degreasing effect is intensified; the yield and the quality of the collagen are improved; moreover, a microorganism can be effectively killed; the corrosion and deterioration prevention effects of the collagen are promoted; the preservation capacity of the collagen is enhanced; further, the molecular chain of the collagen becomes short; the average molecular weight is 1,500 to 3,500 daltons; the collagen is more suitably absorbed by a human body.

The invention discloses a method for preparing collagen oligopeptide by hydrolyzing animal skin by using bacillus subtilis. The method comprises the following steps: carrying out fermentation cultureon bacillus subtilis to obtain bacillus subtilis fermentation liquor, and hydrolyzing animal skin by using the bacillus subtilis fermentation liquor to obtain the collagen oligopeptide which is smallin molecular weight, high in purity and easy to absorb by a human body. In the method provided by the invention, the pretreatment of the animal skin only needs simple mechanical treatment for removingsurface impurities, and does not need chemical pretreatment for degreasing and removing impure protein by using chemical reagents such as salt, alkali, acid and the like, so that the problem of environmental pollution caused by the use of the chemical reagents in the process of preparing the collagen oligopeptide is avoided; and meanwhile, in the method provided by the invention, expensive commercial protease does not need to be added, so that the production cost is remarkably reduced.

A making technology of a dog salmon skin picture comprises the following steps: 1, raw dog salmon surface skin making: cutting the skin of dog salmon from the back, and nondestructively stripping to obtain a dog salmon surface skin; 2, carrying out salt preserving anticorrosion treatment of the raw dog salmon surface skin, immersing the dog salmon surface skin in water, degreasing, liming the degreased dog salmon surface skin, pickling, allowing the internal surface of the dog salmon surface skin to face an acupuncturing template, softly pressing the internal surface of the dog salmon surface skin by acupunctures of the acupuncturing template, and processing by using a tanning agent to form leather; and 3, finishing to obtain dog salmon skin leather, sewing the dog salmon skin leather onto a lining surface through a natural silk thread, and allowing the bone plate of the dog salmon skin leather to face outside to obtain the dog salmon skin picture. The dog salmon skin picture produced in the invention has the characteristics of strong stereo perception, simple and elegant feeling, no deformation, no shedding or fading, easy long-term preservation, high class, and high acceptance by consumers, and the making technology has the advantages of simplicity and low production cost. The making technology can be widely used a production technology of dog salmon skin pictures.

The invention provides a method for preparing a special high-performance acellular dermal matrix dressing. The method specifically comprises the steps of preparation of an acellular dermal matrix and modification of the acellular dermal matrix. Preparation of the acellular dermal matrix comprises the following steps of dermis taking, degreasing, keratinase treatment and cell extraction. According to the method for preparing the special high-performance acellular dermal matrix dressing, cellular constituents are thoroughly washed away, cells are removed through a physical method, and the structure of an extracellular matrix is not substantially damaged; due to the fact that the treatment modes of keratinase and microwave unfreezing are adopted, the spatial arrangement of collagenous fibers in the matrix is looser, and the porosity of the material is improved. Meanwhile, a crosslinking mode is adopted to generate an amidation reaction, antibacterial moisture absorption groups are introduced into the dressing, and the dressing achieves more effects. The product is simple in process, industrial production can be easily achieved, and good economic and social benefits are achieved.