40 results about "Defatting" patented technology

The present invention relates to a method for preparing an acellular dermal matrix derived from a human body, in which the acellular dermal matrix is washed for the second time with an alcohol-based degreasing solution, thereby not only having excellent safety against cytotoxicity, but also being able to effectively remove residual fat.

The invention belongs to the technical field of daily chemicals, and particularly relates to high-temperature and neutral alkyl glycoside sulfonate as well as a preparation method and application thereof. The alkyl glucoside sulfonate provided by the invention is obtained by utilizing hydroxyl activity on a glucoside sugar unit to perform chemical modification, and specifically, 1, 3-dichloro-2-propanol is firstly adopted to perform intramolecular crosslinking on alkyl glucoside, and then 3-chloro-2-hydroxy sodium propanesulfonate is adopted to perform anionic modification. The prepared alkyl glycoside sulfonate has the advantages of being mild, low in irritation, high in hard water resistance, good in foaming performance, low in permeation amount on skin and small in residual amount, and can effectively overcome the defects that alkyl glucoside is high in permeability, high in deproteinization and degreasing capacity, large in damage to skin barriers, weak in thickening effect and the like in personal cleaning products; the method is very suitable for being widely used and popularized in daily chemicals.

The invention relates to a citrus peel extract with a soothing effect and a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a preparation method of a citrus peel extract, which comprises the following steps: degreasing citrus peel by using a supercritical CO2 extraction technology to obtain degreased citrus peel; mixing the degreased citrus peel with water to obtain a citrus peel suspension, and mixing the citrus peel suspension with a compound enzyme preparation for enzymolysis to obtain the citrus peel subjected to enzymolysis; and filtering the orange peel after enzymolysis, collecting filtrate, performing ultrafiltration treatment, and collecting fractions with the molecular weight of less than 10kDa to obtain the orange peel extract. The citrus peel is degreased through a supercritical CO2 extraction technology, volatile grease and fat-soluble impurities in the citrus peel can be removed, the components in the citrus peel are further enzymatically hydrolyzed into small-molecule active substances through enzymolysis with a compound enzyme preparation, skin absorption is facilitated, and the soothing and whitening effects of the citrus peel extract are improved.

The invention relates to the technical field of cotton fabric manufacturing, in particular to a charge transfer and transition bacteriostatic pure cotton non-woven fabric and a manufacturing method thereof.The manufacturing method includes the steps that raw cotton is degreased, opened and carded, base cloth is manufactured through a spunlace method, and the pretreated pure cotton non-woven fabric is obtained after drying and impurity removing; the preparation method comprises the following steps: soaking cotton fibers into a charge transfer transition reagent containing alizarin and waterborne polyurethane, accurately loading through a four-roller unit, and curing through 254nm-365nm ultraviolet light, so that the reagent and the cotton fibers form covalent bonds to realize charge transfer transition. An ultrathin polymer film layer is formed on the cloth cover of the product, million-level electron-hole pairs are contained, the initial bacteriostasis rate exceeds 99%, and a good bacteriostasis effect is still kept after the product is washed with water for 150 times. The antibacterial non-woven fabric adopts a natural antibacterial agent, is free of skin irritation and toxic residues, is environment-friendly in production process, has skin friendliness and mechanical strength, is suitable for medical and hygienic products such as surgical gown and medical dressing, and solves the problems of poor washability, potential safety hazards and the like of the traditional antibacterial non-woven fabric.

The application belongs to the technical field of daily chemicals, and particularly relates to a high-temperature and mild alkyl glucoside sulfonate as well as a preparation method and application thereof. The alkyl glucoside sulfonate provided by the application is obtained by using the hydroxyl activity on the glucose unit of glucoside to perform chemical modification, and specifically, the alkyl glucoside is first subjected to intramolecular cross-linking by using 1,3-dichloro-2-propanol, and then subjected to anionization modification by using 3-chloro-2-hydroxypropanesulfonate sodium. The alkyl glucoside sulfonate prepared by the application has the advantages of mildness, low irritation, strong hard water resistance, good foaming performance, low skin penetration and small residual amount, can effectively solve the defects of strong penetration, strong deproteinization and delipidation, great damage to skin barrier and weak thickening effect of alkyl glucoside in personal cleaning products, and is very suitable for being widely used and popularized in daily chemicals.

The invention provides bright and smooth fragrant bath oil and a preparation method thereof. The bright and smooth fragrant bath oil comprises the following components in parts by weight: 0.05-0.5 part of a chelating agent, 10-20 parts of a humectant, 15-25 parts of a surfactant, 0.1-1 part of a thickening agent, 0.1-1 part of an antioxidant, 0.1-2 parts of an aromatic, 0.1-5 parts of a skin conditioner and 50-75 parts of water. The surfactant is a combination of C14-16 sodium olefin sulfonate, cocamidopropyl betaine, decyl glucoside, lauryl betaine, sodium lauroyl methyl amino propionate and PEG-40 (Polyethylene Glycol) hydrogenated castor oil. The bath oil disclosed by the invention not only has the characteristic of long-acting fragrance, but also has dual effects of cleaning, moistening and repairing, can be quickly emulsified to form a fine and smooth system when encountering water, mildly dissolves grease and dirt on the surface of skin, avoids the problem of excessive degreasing of a traditional cleaning product, and cares a skin sebum film. Meanwhile, dry skin can be deeply moistened, skin barrier lipid can be supplemented, the problems of rough skin and dry itching can be relieved, and the washed skin is kept moist, soft and smooth and does not have a tight and dry feeling.

This invention discloses a method for preparing acellular dermal matrix, belonging to the field of tissue engineering technology. The preparation method includes: selecting pelts from traceable animals aged one to three months, purifying them through steps such as soaking, fleshing, defatting, hair removal, liming, deliming, and softening in a tanning process to obtain acellular dermal matrix. The acellular dermal matrix obtained by this invention removes antigenic components such as cells, hair follicles, and extracellular proteins during the preparation process, minimizing its immunogenicity while preserving the collagen fiber structure in its extracellular matrix. Simultaneously, the preparation method of this invention moderately loosens the collagen fiber bundles. This process not only effectively alleviates the entanglement between fiber bundles but also successfully increases the exposure of hydrophilic groups, giving the acellular dermal matrix excellent hydrophilicity, which is more conducive to cell proliferation and migration.

The invention provides a preparation method of SPF (specific pathogen free) pigskin extracellular matrix (ECM) hydrogel. The preparation method comprises the following steps: S1, collecting SPF pigskin and pretreating; s2, the pretreated SPF pigskin is subjected to degreasing treatment; s3, sterilizing and disinfecting the freeze-dried powder, and then carrying out enzymolysis; s4, adjusting the pH and osmotic pressure of supernate after enzymolysis to obtain a hydrogel product. The source of the hydrogel product does not contain non-biological source materials at all, establishment of a three-dimensional cell culture environment and growth of cultured cells, especially organoids are facilitated, and meanwhile the problems of tumor sources, batch differences, high cost, potential safety hazards and the like of existing hydrogel products such as Matrigel and the like are solved. Experimental results show that the pigskin ECM hydrogel with different concentrations and from different ages can support the growth of intestinal organs, and the number of the organs cultured by the SPF pigskin ECM hydrogel with the concentration of 3 mg / mL is even more than that of Matrigel.

Cosmetic use of defatted almond extracts of Carapa procera from the Meliaceae family intended to be placed in contact with the various superficial parts of the human body in order to reduce pigmentation and signs of aging such as lack of radiance, wrinkles, loss of firmness and elasticity.

The application discloses a rapid detection method of neutral defatting agent, acid solution is quantitatively added in a certain volume of defatting liquid, the higher the defatting agent concentration is, the more acid is consumed, that is, the higher the pH value is, and vice versa, then the measured pH value is nonlinearly polynomially fitted with the defatting agent concentration to obtain a relationship function of both, and then the defatting agent concentration in the defatting liquid on site is calculated by using the relationship function; the application adopts common laboratory equipment and vessels such as a pH meter, an adjustable pipette and a volumetric flask, does not need to use special equipment and devices, is low in cost and simple in operation; the operation process of the application is short, the requirement for the operation experience of personnel is not high, the application is easy to master, the defatting liquid concentration adjustment time is effectively shortened, the work efficiency is improved, and the problem of low accuracy of the neutral defatting agent concentration determined by the weight method and the titration method is solved.

The present application belongs to the technical field of skin care products, and particularly relates to a plant compound cleansing jelly with oil control and acne removal effects and a preparation method thereof. An amino acid surfactant compound system is adopted to effectively remove excess facial oil and dirt while causing little damage to the skin barrier and having no tight feeling after washing, thus solving the problem of'more oil after washing' caused by excessive defatting of traditional soap-based cleansing products. By compounding Lonicera japonica flower extract with plant extracts such as Sophora flavescens, Scutellaria baicalensis and Hamamelis virginiana and PCA zinc, synergistic effects are produced in terms of inhibiting sebum secretion (oil control), inhibiting Propionibacterium acnes (acne removal) and reducing inflammatory reactions (anti-inflammatory), and compared with single components or simple combinations, the effects are more comprehensive and longer-lasting. In addition, the product provided by the present application is in the form of jelly, is refreshing and not sticky, is easy to rinse, and is suitable for daily use of oily and mixed oily skin.

The invention relates to the technical field of collagen peptide extraction, and particularly discloses a preparation method of fresh bovine bone collagen peptide for increasing bone mineral density, which comprises the following steps: step S1, degreasing treatment; step S2, crude extraction; step S3, performing enzymolysis; step S4, performing supercritical extraction; according to the technical scheme, the extraction promoting liquid is added in the crude extraction process, meanwhile, ultrasonic-assisted synchronous microwave radiation is matched, calcium lactate, lecithin, chlorogenic acid and compound enzyme are added in the enzymolysis process, and the purity and yield of the fresh bovine bone collagen peptide are improved through the synergistic effect of all the procedures.

The application belongs to the technical field of cosmetic preparation, and discloses a micro-nannochloropsis extract for after-sun repair of deep-skinned people, a preparation method and application thereof. The method comprises the following steps: defatting and decolorizing filtration of micro-nannochloropsis powder, enzyme hydrolysis of the filter residue after addition of cellulase liquid, and then water extraction under acidic conditions with pH of 1-3 and at 80 DEG C, cooling and filtration, pH of 8-10 alkaline condition and 80 DEG C extraction of the obtained filter residue, cooling and filtration, combined filtration of the filtrates obtained by the two times of filtration, ultrafiltration treatment, 95% ethanol precipitation, centrifugation to obtain supernatant, reduced pressure concentration, dilution of the obtained concentrated solution, and then loading onto a pre-activated SPE column, elution with 60%, 70% and 80% ethanol in sequence, combined elution, and reduced pressure concentration to obtain the micro-nannochloropsis extract. The extract has the effects of enhancing skin repair and anti-inflammation, is especially suitable for after-sun care of deep-skinned people, limits melanin production, slows down the secretion speed of oil, and repairs the skin barrier.

The utility model discloses a degreasing auxiliary tank structure for quickly cleaning floating foam, which comprises an auxiliary tank body, an overflow component is arranged in the auxiliary tank body, the overflow component comprises two overflow plates arranged between opposite side walls of the auxiliary tank body, the lower ends of the two overflow plates are connected with a bottom plate, and the bottom plate is connected with a bottom plate. The overflow plate, the side wall of the auxiliary groove body and the bottom plate jointly define an overflow groove with the top open, and a discharging opening communicated with the overflow groove is formed in the side wall of the auxiliary groove body. According to the degreasing auxiliary tank, the floating foam in the degreasing auxiliary tank can be removed, the floating foam is prevented from being accumulated too high and overflowing out of the degreasing auxiliary tank, and the working environment is prevented from being polluted by the floating foam.

The invention discloses a preparation process of an efficient acellular dermal matrix, which comprises the following steps: pretreating animal fetal skin to obtain dermal tissue, sequentially carrying out specific acellular treatment and degreasing treatment processes, and subsequently carrying out crosslinking, freeze-drying and sterilization to obtain the acellular dermal matrix. According to the preparation process of the acellular dermal matrix, impurities and immunogenic substances can be effectively removed, and meanwhile, the activity of the extracellular matrix is protected to the maximum extent; the obtained acellular dermal matrix product is complete in collagen triple helix structure, low in residues of DNA, alpha-Gal antigens and the like, extremely low in fat content, free of cytotoxicity and high in safety.

The invention discloses a cosmetic containing a vinasse extracting solution as well as a preparation method and application of the cosmetic. The composition comprises the following components in parts by weight: 10 parts of a vinasse extracting solution, 0.05-1 part of sodium hyaluronate, 12-22 parts of a humectant and 0.1-1 part of a thickening agent, wherein the thickening agent is selected from tremella aurantialba polysaccharide. According to the method, the vinasse powder is subjected to degreasing treatment and ultrasonic extraction, so that the content of active ingredients such as polysaccharides, vitamins, polyphenols and amino acids in the vinasse extracting solution is increased. A composition or a cosmetic obtained by mixing the vinasse extracting solution, sodium hyaluronate, a humectant and a thickening agent not only can remarkably enhance the water locking capacity of skin and achieve efficient and long-acting moisturizing, but also can repair a skin barrier, and has a remarkable oil control effect at the same time. In addition, the composition or the cosmetic of the present invention has excellent stability and is easy to store and use. According to the method, high-value utilization of vinasse resources is realized, a new way is provided for development of green skin care products, and the method has a wide application prospect.

The invention discloses a preparation method of portulaca oleracea polysaccharide. The preparation method comprises the steps of degreasing, water extraction, deproteinization, alcohol precipitation, drying, crushing and the like. The preparation method of the portulaca oleracea polysaccharide has the remarkable advantages of simple and convenient process, low cost, safety, high efficiency and the like. Pharmacological experiments prove that the portulaca oleracea polysaccharide prepared by the preparation method disclosed by the invention has obvious effects of resisting ulcerative colitis and secondary osteoporosis, and can be used for preparing healthy products, including medicines, health foods and foods, for resisting ulcerative colitis and secondary osteoporosis.

The application discloses a preparation process of efficient acellular dermal matrix, and the acellular dermal matrix is prepared through the following steps: obtaining dermal tissue through pretreatment of animal fetal skin, and then performing specific acellular treatment and defatting treatment processes, and finally performing crosslinking, freeze-drying and sterilization. The acellular dermal matrix preparation process can effectively remove impurities and immunogenic substances, and at the same time, the activity of extracellular matrix is maximally protected. The obtained acellular dermal matrix product has complete triple-helix structure of collagen, low residues of DNA and alpha-Gal antigen, extremely low fat content, no cytotoxicity and high safety.

The invention discloses a preparation method of peony seed peptide, which comprises the following steps: (1) mixing degreased peony seed meal with water, adjusting the pH value to alkalinity, heating, stirring and extracting to obtain an alkali extract; (2) adjusting the pH value of the alkali extract back to be alkaline, and adding alkaline protease for enzymolysis to obtain enzymatic hydrolysate; (3) carrying out enzyme deactivation treatment on the enzymatic hydrolysate, adjusting the pH value, centrifuging, and collecting supernate; (4) filtering the supernate to obtain clear peptide liquid; (5) performing ultrafiltration treatment and desalination treatment on the clear peptide liquid, and enriching components to obtain a peony seed peptide solution; and (6) drying and sterilizing the peony seed peptide solution to obtain the peony seed peptide. Reliable technical support is provided for industrial application of the peony seed peptide, and deep development of the peony seed peptide in the fields of high-end medicines for resisting light aging and the like and / or skin care is expected to be promoted.

The invention provides a biological dressing as well as a preparation method and application thereof. The preparation method comprises the following steps: thawing in still water; unhairing and degreasing; cutting and segmenting; fine trimming and punching; performing iodophor soaking treatment; soaking in purified water; soaking in a mixed solution of protease and alkali metal hydroxide; soaking in purified water; antibacterial agent brushing treatment is carried out; and packaging and irradiating. The product obtained by the invention can be used for purifying, cleaning and protecting wound surfaces, promoting granulation tissue growth, covering granulation wound surfaces, chronic ulcers and bedsores, shortening the healing time and improving the healing effect.

The invention relates to the technical field of biomedical materials, in particular to a collagen preparation method which comprises the following steps: performing non-collagen impurity removal and degreasing treatment on animal skin tissues to obtain pretreated skin tissues; performing enzymolysis and salting-out on the pretreated skin tissue to obtain a collagen solution; diluting the collagen solution through an acid extraction buffer solution to obtain a diluted collagen solution; adding a salt solution at the temperature of 0-4 DEG C into the diluted collagen solution, and stirring to obtain a collagen solution to be purified; and purifying the collagen solution to be purified through the resin filler to obtain the collagen. Under the conditions of no organic solvent and no denaturing agent, the collagen can be efficiently purified on the premise of keeping the natural triple-helix structure of the collagen and ensuring the biological activity by combining the low-temperature low-ionic-strength pretreatment with the resin purification technology, and the long-standing technical bottleneck that the purification efficiency and the biological activity are difficult to consider at the same time in the industry is solved.

The present disclosure relates to a method of obtaining a decellularized dermal matrix from a skin graft of a rabbit, hare or beaver, the method comprising the steps of: degreasing the skin graft using a hydrocarbon solvent, perchloroethylene, a modified alcohol or supercritical carbon dioxide, or a combination thereof; liming the degreased graft by immersing the graft in a solution comprising calcium hydroxide, sodium hydroxide, hydrogen peroxide, or a mixture thereof; fleshing the skin graft; optionally, de-ashing the skin graft; incubating the skin graft in an ethylenediamine tetraacetic acid solution; the skin graft is treated with the detergent solution for 30 minutes to 3 hours to obtain a decellularized dermal matrix. The present disclosure also relates to a decellularized dermal matrix obtained by said method; a composition comprising the decellularized dermal matrix for use in a medicament or veterinary medicament; and a dermal-epidermal skin substitute scaffold comprising the disclosed decellularized dermal matrix.

This invention belongs to the field of bioactive peptide preparation technology, specifically relating to a method for preparing frog oil polypeptides for preventing skin aging. The method includes: pretreating dried frog oil through segmented hydration and rehydration, defatting and deodorization, and calcium salt confinement; then adding a complex protease for segmented pulsed ultrasonic enzymatic hydrolysis; after enzymatic hydrolysis, the mixture undergoes enzyme inactivation, centrifugation to remove impurities, and dual-stage ultrafiltration at 2kDa and 500Da to collect a 0.5–2kDa frog oil polypeptide initial enrichment solution, which is then purified by macroporous adsorption resin and separated by gel chromatography, followed by freeze-drying to obtain the frog oil polypeptides. This invention, through the combination of calcium salt confinement pretreatment and segmented pulsed ultrasonic enzymatic hydrolysis, allows frog oil proteins to undergo controlled enzymatic cleavage under moderate expansion conditions, which is beneficial for improving the formation stability and enrichment consistency of the 0.5–2kDa target polypeptide component. The obtained frog oil polypeptides can serve as active components for preventing skin aging.

The application provides a giant salamander skin non-denatured collagen hydrogel dressing and a preparation method thereof. The giant salamander skin is used as a raw material, only sodium carbonate degreasing and mild pepsin extraction are performed, and then the giant salamander skin non-denatured collagen is obtained. The giant salamander skin non-denatured collagen is compounded with acceptable hydrogel accessories to form a hydrogel dressing with suitable swelling rate and degradability, so that excellent skin repair and anti-aging effects can be achieved without adding exogenous growth factors, and the dressing formula is greatly simplified. Compared with a traditional collagen extraction process, the process is simple, the cost is low, the collagen yield is much higher than that of traditional acid extraction and enzyme extraction methods, and the collagen retention rate can reach more than 90%. Furthermore, the high-value utilization of the giant salamander processing by-products is realized, the raw material source of the functional collagen is expanded, and the economic and environmental values are double.

The application discloses an elastin peptide and a preparation process thereof, and relates to the technical field of deep processing. The elastin peptide and the preparation process thereof are designed in the application. Through specific pretreatment degreasing process and two-stage enzymolysis process, the influence of fat substances in the elastin peptide on the use effect of the elastin peptide is greatly reduced, the biological activity of the elastin peptide is retained, the contents of desmosine and isodesmosine are improved, the elastin peptide is applied to skin anti-wrinkle, the skin elasticity can be obviously enhanced, and good anti-wrinkle effect can be achieved, and the elastin peptide has good application value.

Provided are a biological patch, a preparation method therefor, and use thereof. The biological patch is a fish skin-based biological patch, and contains a first fatty acid and a second fatty acid. A mass percentage of the first fatty acid in the biological patch is greater than or equal to 1.4%, and a mass percentage of the second fatty acid in the biological patch is less than or equal to 2.0%. The first fatty acid includes at least two of butyric acid, caprylic acid, lauric acid, myristic acid, pentadecanoic acid, etc. The second fatty acid includes an ω-6 unsaturated fatty acid. The biological patch includes collagen fibers with a certain diameter, and the biological patch includes micropores with a certain pore size and a certain porosity. The preparation method for the biological patch includes degreasing, pigment removal, and decellularization. The obtained biological patch is used for repairing soft tissue or used as a skin repair dressing. The biological patch can reduce or eliminate inflammatory response of an administration site, and exhibit good mechanical performance and degradation performance.

The application provides a preparation method of giant salamander skin in-situ collagen hydrogel dressing and application thereof in promoting burn wound healing, and belongs to the technical field of wound repair dressing. The in-situ collagen hydrogel dressing prepared from 1-3-year-old giant salamander skin through pretreatment, decolorization, removal of impure protein, degreasing and other steps has a natural three-dimensional honeycomb porous structure. The water vapor transmission rate of the dressing is 2000-2500 g / (m 2 24h), the dressing has excellent water retention and dynamic fitting properties, can realize efficient drug loading and slow release, has a significant bacteriostatic effect on escherichia coli and staphylococcus aureus, and has no cytotoxicity. Animal experiments show that the dressing can effectively reduce wound inflammation, promote granulation tissue growth and re-epithelialization, and significantly improve the healing speed and quality of deep Ⅱ degree burn wounds and other wounds. The application has simple process, good biocompatibility, good mechanical and functional adaptability, and is suitable for the repair and nursing of infected wounds and burn wounds, and has good clinical transformation and application prospect.

The invention provides an artificial dermis material and a preparation method thereof.The preparation method comprises the following steps that pig dermis tissue is treated with high-concentration alkali liquor, and porous dermis tissue is obtained; sequentially performing degreasing treatment and drying treatment on the porous dermal tissue to obtain a porous acellular dermal matrix; adding the micronized pig dermal matrix, gelatin and honey into water, and uniformly mixing to obtain a composite adhesive liquid; and laying the composite adhesive liquid on one side of a porous acellular dermal matrix, and sequentially carrying out freezing fixation and vacuum drying to obtain the artificial dermal material. Therefore, the obtained artificial dermis material is closer to a physiological structure, the upper layer of dermis can provide mechanical support and ventilation functions, and the lower layer of dermis can quickly absorb tissue penetrating fluid to form a weakly acidic gel protective layer by utilizing high osmotic pressure of honey at the initial stage of wound formation.

The invention provides an injection for dermis tissue filling and a preparation method and application thereof. The filling injection is prepared from 1 mg / mL to 35 mg / mL of I type collagen and 0.5 mg / mL to 10 mg / mL of silk fibroin; wherein the I-type collagen is extracted from omentum majus suis and is obtained by performing pretreatment, cleaning, virus inactivation, degreasing, decellularization, primary freeze-drying, enzymolysis, purification and secondary freeze-drying on the omentum majus suis. When the injection prepared from the filler is used for filling the dermis part, the regeneration of the autologous collagen can be effectively promoted, and the filling durability is ensured.

This invention relates to the field of skincare technology, and in particular to an extraction process for silkworm pupae polypeptides and a repair mask composition. The extraction process includes: defatting silkworm pupae powder using supercritical CO2 extraction to obtain defatted silkworm pupae powder; mixing the defatted silkworm pupae powder, a eutectic solvent, and an antioxidant, and then performing a cell-wall breaking process to obtain a cell-wall broken suspension; adding a complex enzyme to the cell-wall broken suspension and sequentially performing enzymatic hydrolysis, enzyme inactivation, and centrifugation to obtain a supernatant; the complex enzyme includes papain, bromelain, and neutral protease; filtering the supernatant once through a 30kDa filter membrane, collecting the filtrate, and then filtering it a second time through a 10kDa filter membrane to obtain the silkworm pupae polypeptide extract. The silkworm pupae polypeptide extract obtained by the extraction process of this invention has excellent DPPH free radical scavenging rate and no solvent residue, and can be directly used as a component of a repair mask, suitable for sensitive skin and skin damaged after cosmetic procedures.