22 results about "Defatting" patented technology

The invention relates to a citrus peel extract with a soothing effect and a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a preparation method of a citrus peel extract, which comprises the following steps: degreasing citrus peel by using a supercritical CO2 extraction technology to obtain degreased citrus peel; mixing the degreased citrus peel with water to obtain a citrus peel suspension, and mixing the citrus peel suspension with a compound enzyme preparation for enzymolysis to obtain the citrus peel subjected to enzymolysis; and filtering the orange peel after enzymolysis, collecting filtrate, performing ultrafiltration treatment, and collecting fractions with the molecular weight of less than 10kDa to obtain the orange peel extract. The citrus peel is degreased through a supercritical CO2 extraction technology, volatile grease and fat-soluble impurities in the citrus peel can be removed, the components in the citrus peel are further enzymatically hydrolyzed into small-molecule active substances through enzymolysis with a compound enzyme preparation, skin absorption is facilitated, and the soothing and whitening effects of the citrus peel extract are improved.

The invention relates to the technical field of cotton fabric manufacturing, in particular to a charge transfer and transition bacteriostatic pure cotton non-woven fabric and a manufacturing method thereof.The manufacturing method includes the steps that raw cotton is degreased, opened and carded, base cloth is manufactured through a spunlace method, and the pretreated pure cotton non-woven fabric is obtained after drying and impurity removing; the preparation method comprises the following steps: soaking cotton fibers into a charge transfer transition reagent containing alizarin and waterborne polyurethane, accurately loading through a four-roller unit, and curing through 254nm-365nm ultraviolet light, so that the reagent and the cotton fibers form covalent bonds to realize charge transfer transition. An ultrathin polymer film layer is formed on the cloth cover of the product, million-level electron-hole pairs are contained, the initial bacteriostasis rate exceeds 99%, and a good bacteriostasis effect is still kept after the product is washed with water for 150 times. The antibacterial non-woven fabric adopts a natural antibacterial agent, is free of skin irritation and toxic residues, is environment-friendly in production process, has skin friendliness and mechanical strength, is suitable for medical and hygienic products such as surgical gown and medical dressing, and solves the problems of poor washability, potential safety hazards and the like of the traditional antibacterial non-woven fabric.

The application belongs to the technical field of daily chemicals, and particularly relates to a high-temperature and mild alkyl glucoside sulfonate as well as a preparation method and application thereof. The alkyl glucoside sulfonate provided by the application is obtained by using the hydroxyl activity on the glucose unit of glucoside to perform chemical modification, and specifically, the alkyl glucoside is first subjected to intramolecular cross-linking by using 1,3-dichloro-2-propanol, and then subjected to anionization modification by using 3-chloro-2-hydroxypropanesulfonate sodium. The alkyl glucoside sulfonate prepared by the application has the advantages of mildness, low irritation, strong hard water resistance, good foaming performance, low skin penetration and small residual amount, can effectively solve the defects of strong penetration, strong deproteinization and delipidation, great damage to skin barrier and weak thickening effect of alkyl glucoside in personal cleaning products, and is very suitable for being widely used and popularized in daily chemicals.

The invention provides bright and smooth fragrant bath oil and a preparation method thereof. The bright and smooth fragrant bath oil comprises the following components in parts by weight: 0.05-0.5 part of a chelating agent, 10-20 parts of a humectant, 15-25 parts of a surfactant, 0.1-1 part of a thickening agent, 0.1-1 part of an antioxidant, 0.1-2 parts of an aromatic, 0.1-5 parts of a skin conditioner and 50-75 parts of water. The surfactant is a combination of C14-16 sodium olefin sulfonate, cocamidopropyl betaine, decyl glucoside, lauryl betaine, sodium lauroyl methyl amino propionate and PEG-40 (Polyethylene Glycol) hydrogenated castor oil. The bath oil disclosed by the invention not only has the characteristic of long-acting fragrance, but also has dual effects of cleaning, moistening and repairing, can be quickly emulsified to form a fine and smooth system when encountering water, mildly dissolves grease and dirt on the surface of skin, avoids the problem of excessive degreasing of a traditional cleaning product, and cares a skin sebum film. Meanwhile, dry skin can be deeply moistened, skin barrier lipid can be supplemented, the problems of rough skin and dry itching can be relieved, and the washed skin is kept moist, soft and smooth and does not have a tight and dry feeling.

The invention provides a preparation method of SPF (specific pathogen free) pigskin extracellular matrix (ECM) hydrogel. The preparation method comprises the following steps: S1, collecting SPF pigskin and pretreating; s2, the pretreated SPF pigskin is subjected to degreasing treatment; s3, sterilizing and disinfecting the freeze-dried powder, and then carrying out enzymolysis; s4, adjusting the pH and osmotic pressure of supernate after enzymolysis to obtain a hydrogel product. The source of the hydrogel product does not contain non-biological source materials at all, establishment of a three-dimensional cell culture environment and growth of cultured cells, especially organoids are facilitated, and meanwhile the problems of tumor sources, batch differences, high cost, potential safety hazards and the like of existing hydrogel products such as Matrigel and the like are solved. Experimental results show that the pigskin ECM hydrogel with different concentrations and from different ages can support the growth of intestinal organs, and the number of the organs cultured by the SPF pigskin ECM hydrogel with the concentration of 3 mg / mL is even more than that of Matrigel.

Cosmetic use of defatted almond extracts of Carapa procera from the Meliaceae family intended to be placed in contact with the various superficial parts of the human body in order to reduce pigmentation and signs of aging such as lack of radiance, wrinkles, loss of firmness and elasticity.

The application discloses a rapid detection method of neutral defatting agent, acid solution is quantitatively added in a certain volume of defatting liquid, the higher the defatting agent concentration is, the more acid is consumed, that is, the higher the pH value is, and vice versa, then the measured pH value is nonlinearly polynomially fitted with the defatting agent concentration to obtain a relationship function of both, and then the defatting agent concentration in the defatting liquid on site is calculated by using the relationship function; the application adopts common laboratory equipment and vessels such as a pH meter, an adjustable pipette and a volumetric flask, does not need to use special equipment and devices, is low in cost and simple in operation; the operation process of the application is short, the requirement for the operation experience of personnel is not high, the application is easy to master, the defatting liquid concentration adjustment time is effectively shortened, the work efficiency is improved, and the problem of low accuracy of the neutral defatting agent concentration determined by the weight method and the titration method is solved.

The invention relates to the technical field of collagen peptide extraction, and particularly discloses a preparation method of fresh bovine bone collagen peptide for increasing bone mineral density, which comprises the following steps: step S1, degreasing treatment; step S2, crude extraction; step S3, performing enzymolysis; step S4, performing supercritical extraction; according to the technical scheme, the extraction promoting liquid is added in the crude extraction process, meanwhile, ultrasonic-assisted synchronous microwave radiation is matched, calcium lactate, lecithin, chlorogenic acid and compound enzyme are added in the enzymolysis process, and the purity and yield of the fresh bovine bone collagen peptide are improved through the synergistic effect of all the procedures.

The utility model discloses a degreasing auxiliary tank structure for quickly cleaning floating foam, which comprises an auxiliary tank body, an overflow component is arranged in the auxiliary tank body, the overflow component comprises two overflow plates arranged between opposite side walls of the auxiliary tank body, the lower ends of the two overflow plates are connected with a bottom plate, and the bottom plate is connected with a bottom plate. The overflow plate, the side wall of the auxiliary groove body and the bottom plate jointly define an overflow groove with the top open, and a discharging opening communicated with the overflow groove is formed in the side wall of the auxiliary groove body. According to the degreasing auxiliary tank, the floating foam in the degreasing auxiliary tank can be removed, the floating foam is prevented from being accumulated too high and overflowing out of the degreasing auxiliary tank, and the working environment is prevented from being polluted by the floating foam.

The application discloses a preparation process of efficient acellular dermal matrix, and the acellular dermal matrix is prepared through the following steps: obtaining dermal tissue through pretreatment of animal fetal skin, and then performing specific acellular treatment and defatting treatment processes, and finally performing crosslinking, freeze-drying and sterilization. The acellular dermal matrix preparation process can effectively remove impurities and immunogenic substances, and at the same time, the activity of extracellular matrix is maximally protected. The obtained acellular dermal matrix product has complete triple-helix structure of collagen, low residues of DNA and alpha-Gal antigen, extremely low fat content, no cytotoxicity and high safety.

The invention discloses a preparation method of peony seed peptide, which comprises the following steps: (1) mixing degreased peony seed meal with water, adjusting the pH value to alkalinity, heating, stirring and extracting to obtain an alkali extract; (2) adjusting the pH value of the alkali extract back to be alkaline, and adding alkaline protease for enzymolysis to obtain enzymatic hydrolysate; (3) carrying out enzyme deactivation treatment on the enzymatic hydrolysate, adjusting the pH value, centrifuging, and collecting supernate; (4) filtering the supernate to obtain clear peptide liquid; (5) performing ultrafiltration treatment and desalination treatment on the clear peptide liquid, and enriching components to obtain a peony seed peptide solution; and (6) drying and sterilizing the peony seed peptide solution to obtain the peony seed peptide. Reliable technical support is provided for industrial application of the peony seed peptide, and deep development of the peony seed peptide in the fields of high-end medicines for resisting light aging and the like and / or skin care is expected to be promoted.

The invention provides a biological dressing as well as a preparation method and application thereof. The preparation method comprises the following steps: thawing in still water; unhairing and degreasing; cutting and segmenting; fine trimming and punching; performing iodophor soaking treatment; soaking in purified water; soaking in a mixed solution of protease and alkali metal hydroxide; soaking in purified water; antibacterial agent brushing treatment is carried out; and packaging and irradiating. The product obtained by the invention can be used for purifying, cleaning and protecting wound surfaces, promoting granulation tissue growth, covering granulation wound surfaces, chronic ulcers and bedsores, shortening the healing time and improving the healing effect.

The invention relates to the technical field of biomedical materials, in particular to a collagen preparation method which comprises the following steps: performing non-collagen impurity removal and degreasing treatment on animal skin tissues to obtain pretreated skin tissues; performing enzymolysis and salting-out on the pretreated skin tissue to obtain a collagen solution; diluting the collagen solution through an acid extraction buffer solution to obtain a diluted collagen solution; adding a salt solution at the temperature of 0-4 DEG C into the diluted collagen solution, and stirring to obtain a collagen solution to be purified; and purifying the collagen solution to be purified through the resin filler to obtain the collagen. Under the conditions of no organic solvent and no denaturing agent, the collagen can be efficiently purified on the premise of keeping the natural triple-helix structure of the collagen and ensuring the biological activity by combining the low-temperature low-ionic-strength pretreatment with the resin purification technology, and the long-standing technical bottleneck that the purification efficiency and the biological activity are difficult to consider at the same time in the industry is solved.

The present disclosure relates to a method of obtaining a decellularized dermal matrix from a skin graft of a rabbit, hare or beaver, the method comprising the steps of: degreasing the skin graft using a hydrocarbon solvent, perchloroethylene, a modified alcohol or supercritical carbon dioxide, or a combination thereof; liming the degreased graft by immersing the graft in a solution comprising calcium hydroxide, sodium hydroxide, hydrogen peroxide, or a mixture thereof; fleshing the skin graft; optionally, de-ashing the skin graft; incubating the skin graft in an ethylenediamine tetraacetic acid solution; the skin graft is treated with the detergent solution for 30 minutes to 3 hours to obtain a decellularized dermal matrix. The present disclosure also relates to a decellularized dermal matrix obtained by said method; a composition comprising the decellularized dermal matrix for use in a medicament or veterinary medicament; and a dermal-epidermal skin substitute scaffold comprising the disclosed decellularized dermal matrix.

The application discloses an elastin peptide and a preparation process thereof, and relates to the technical field of deep processing. The elastin peptide and the preparation process thereof are designed in the application. Through specific pretreatment degreasing process and two-stage enzymolysis process, the influence of fat substances in the elastin peptide on the use effect of the elastin peptide is greatly reduced, the biological activity of the elastin peptide is retained, the contents of desmosine and isodesmosine are improved, the elastin peptide is applied to skin anti-wrinkle, the skin elasticity can be obviously enhanced, and good anti-wrinkle effect can be achieved, and the elastin peptide has good application value.

Provided are a biological patch, a preparation method therefor, and use thereof. The biological patch is a fish skin-based biological patch, and contains a first fatty acid and a second fatty acid. A mass percentage of the first fatty acid in the biological patch is greater than or equal to 1.4%, and a mass percentage of the second fatty acid in the biological patch is less than or equal to 2.0%. The first fatty acid includes at least two of butyric acid, caprylic acid, lauric acid, myristic acid, pentadecanoic acid, etc. The second fatty acid includes an ω-6 unsaturated fatty acid. The biological patch includes collagen fibers with a certain diameter, and the biological patch includes micropores with a certain pore size and a certain porosity. The preparation method for the biological patch includes degreasing, pigment removal, and decellularization. The obtained biological patch is used for repairing soft tissue or used as a skin repair dressing. The biological patch can reduce or eliminate inflammatory response of an administration site, and exhibit good mechanical performance and degradation performance.

This invention relates to the field of skincare technology, and in particular to an extraction process for silkworm pupae polypeptides and a repair mask composition. The extraction process includes: defatting silkworm pupae powder using supercritical CO2 extraction to obtain defatted silkworm pupae powder; mixing the defatted silkworm pupae powder, a eutectic solvent, and an antioxidant, and then performing a cell-wall breaking process to obtain a cell-wall broken suspension; adding a complex enzyme to the cell-wall broken suspension and sequentially performing enzymatic hydrolysis, enzyme inactivation, and centrifugation to obtain a supernatant; the complex enzyme includes papain, bromelain, and neutral protease; filtering the supernatant once through a 30kDa filter membrane, collecting the filtrate, and then filtering it a second time through a 10kDa filter membrane to obtain the silkworm pupae polypeptide extract. The silkworm pupae polypeptide extract obtained by the extraction process of this invention has excellent DPPH free radical scavenging rate and no solvent residue, and can be directly used as a component of a repair mask, suitable for sensitive skin and skin damaged after cosmetic procedures.

The invention relates to a composition with anti-sugar and anti-aging effects and application thereof. The composition is prepared from the following components in parts by mass: 10 to 30 parts of plukenetia volubilis linneo protein fermentation peptide, 20 to 60 parts of guava extract and 5 to 20 parts of pyrroloquinoline quinone disodium salt. The plukenetia volubilis linneo protein fermentation peptide is prepared by a method comprising the following steps: mixing plukenetia volubilis linneo meal with an organic solvent, and performing degreasing treatment under a subcritical condition to obtain degreased plukenetia volubilis linneo; mixing the degreased plukenetia volubilis linneo with water, and performing micro-jet pretreatment to obtain pretreated plukenetia volubilis linneo; sequentially performing ultrasonic extraction, water bath extraction and centrifugation on the pretreated sacha inchi, taking supernate and settling to obtain sacha inchi protein; performing mixed fermentation on the sacha inchi protein and bacillus subtilis to obtain fermentation liquor; and performing ultrasonic extraction, filtration and drying on the fermentation liquor to obtain the traditional Chinese medicine composition. The composition prepared by the invention has anti-oxidation, anti-saccharification and anti-aging effects, and provides a functional component source for the field of skin care products.

This invention discloses a method for preparing fish bones for fishbone painting, comprising: cooking the fish to separate the flesh from the bones; peeling the muscle tissue using a sterilized fine peeling tool in a sterile environment; sequentially performing degreasing and bleaching treatments; and washing and drying to obtain fish bones for fishbone painting. This invention avoids bone contamination and structural damage through fine peeling in a sterile environment. Combined with multi-stage degreasing and controlled bleaching processes, it effectively removes muscle, lipids, and odors, resulting in fish bones with high whiteness, intact structure, strong antioxidant properties, and resistance to yellowing and mold. Optionally, they may also have a natural floral fragrance. This invention provides high-quality raw materials for fishbone painting and other bone art pieces, suitable for industrial production.

The application discloses a kind of bacteriostatic leather fabric and preparation method thereof, first animal raw hide is treated with degreasing agent solution and is degreased, and the degreasing skin is obtained;First washing, neutralization, second washing, and the second washing skin is obtained;Then the second washing skin is completely immersed in bacteriostatic solution, and the bacteriostatic treatment skin is obtained by impregnation treatment;The bacteriostatic treatment skin is retanned using retanning agent, and the retanned treatment skin is obtained;Finally, the retanned treatment skin is fatliquored using fatliquoring agent, dyed, and the bacteriostatic leather fabric is obtained by fixing.The leather fabric obtained by the application has excellent bacteriostatic effect, and prolongs the service life of leather fabric.

The application provides a medical grade tissue-like natural collagen and a preparation method and application thereof, and relates to biomedical materials. The application provides a preparation method of the medical grade tissue-like natural collagen, which comprises the following steps: removing subcutaneous tissue of animal skin, soaking in physiological saline, sequentially performing defatting treatment, hair removal treatment, swelling removal treatment, acid swelling treatment, ball milling, adjusting pH value to 7-7.4, performing solid-liquid separation, and drying the obtained solid component to obtain the medical grade tissue-like natural collagen. The medical grade tissue-like natural collagen prepared by the application has good biocompatibility, suitable biodegradability, good mechanical properties, excellent thermal stability and structural stability, low antigenicity and easy preservation, can quickly and effectively stop bleeding, promote wound healing and repair, and is an ideal material of biomedical materials.

The invention relates to the technical field of biology, and particularly discloses wood frog collagen peptide as well as a preparation method and application thereof. The wood frog collagen peptide is extracted from wood frog skin and prepared through the steps of degreasing, ultrasonic treatment, enzymolysis, purification and the like, three polypeptide components I, II and III are separated through size exclusion chromatography, and the amino acid sequences of the three polypeptide components I, II and III are SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 respectively. Experiments show that the polypeptides have remarkable antioxidant activity, the activity of the component II is equivalent to that of ascorbic acid, the activity of the component III is superior to that of ascorbic acid, and the polypeptides have application potential in the fields of medicines, nutrition and health care and cosmetics. The preparation method is simple and efficient, the obtained polypeptide is high in purity, and a new way is provided for high-value utilization of wood frog resources.