82 results about "Extracellular proteins" patented technology

The invention discloses a lysosome targeted chimera based on Sortilin and application thereof. The lysosome targeted chimera comprises a ligand molecule of a lysosome targeted receptor and a ligand molecule of a targeted protein to be degraded, wherein the ligand molecule and the ligand molecule are connected through a linker; the lysosome targeting receptor is Sortilin, and the ligand molecule of the lysosome targeting receptor is a polypeptide targeting the Sortilin; the protein to be degraded is membrane protein or extracellular protein. Furthermore, the polypeptide of the targeted Sortilin is connected with an enzyme response sequence and an integrin receptor targeting sequence. According to the invention, the Sortilin-combined ligand neurotensin is functionally coupled with the neutralizing antibody or polypeptide of overexpression protein in targeted tumor or inflammatory diseases, and the Sortilin-combined ligand neurotensin has obvious treatment gain in malignant solid tumor and psoriasis models. The discovery promotes the transformation potential of the lysosome participating in the biological agent, and provides a dual strategy for realizing accurate degradation through receptor recruitment and microenvironment induction.

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

The invention relates to a bifunctional compound and application thereof in targeted degradation of protein, the compound comprises a target protein binding compound and a cell-penetrating peptide which are connected, and the target protein binding compound is a compound capable of binding with cell membrane protein or extracellular protein or a derivative thereof; the target protein binding compound and the cell-penetrating peptide are connected through a linker or directly connected. According to the invention, it is found for the first time that specific extracellular proteins or cell membrane proteins can enter cells in an endocytosis manner and are degraded through lysosomes by using cell-penetrating peptides and small molecule conjugates. The method disclosed by the invention has the characteristic of delivering bioactive macromolecules through endosome wrapping without depending on a single receptor, and the effect of degrading the target protein by using the cells can be realized as long as specific binding micromolecules of the protein to be degraded can be found and coupled with the cell-penetrating peptide; a widely applicable strategy is provided for degradation of extracellular proteins and cell membrane proteins.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

The invention discloses CD44-mediated self-assembled nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of biology. The CD44-mediated self-assembled nanoparticles are formed by self-assembling polylysine with a phenylboronic acid side chain and dopamine-modified hyaluronic acid through electrostatic interaction, and the side-chain phenylboronic acid of the polylysine is connected with the side-chain dopamine on the hyaluronic acid through a covalent bond. The surface of the nanoparticle is connected with a target protein binding unit through a linker, and the target protein binding unit is used for binding a target protein. The synthesis process is simple, the components are safe, the prepared CD44-mediated self-assembled nano material can efficiently degrade tumor cell extracellular proteins such as PD-L1 and HER2, the specificity is high, the degradation rate is high, and the CD44-mediated self-assembled nano material has a very good application prospect in the field of treatment of diseases such as tumors.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

The invention discloses a preparation process of acinetobacter baumannii extracellular protein OmpA, which comprises the following steps: (1) seeds are inoculated into a seed culture medium for primary culture, then secondary culture is carried out, IPTG (isopropyl-beta-d-thiogalactoside) is added, induction is carried out for 3-5 hours, and the culture medium comprises yeast powder, a nitrogen source and salt; (2) centrifuging, collecting thalli, and crushing the thalli; (3) dissolving and solubilizing the inclusion body, namely dissolving 1.0 g of precipitate in 10mL of solution, centrifuging, and collecting supernate; (4) anion amp; carrying out cation chromatography; (5) dilution and renaturation: diluting with a renaturation solution with the pH value of 7.5 by using 20mM PB, renaturating overnight, centrifuging, and waiting for column loading; (6) cation chromatography; and (7) dialyzing and changing the solution. The protein purity (SDS-PAGE) of the acinetobacter baumannii extracellular protein OmpA prepared by the method disclosed by the invention is more than 99%, and the protein yield is 15.4 mg / g inclusion body.

Extracellular protein degraders and compositions are provided that have a potent asialoglycoprotein receptor (ASGPR) Binding Ligand bound to an Extracellular Protein Targeting Ligand for the selective degradation of the Target Extracellular Protein, for example immunoglobulin in vivo to treat disorders mediated by the extracellular protein.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

Disclosed herein are methods for preparing a biological sample for single-cell analysis which will simultaneously provide single-cell RNA, single-cell extracellular protein, single-cell cytoplasmic protein, and single-cell transcription factor readouts while preserving cellular heterogeneity. Some methods disclosed herein allow for the detection of proteins including the detection of the level of proteins and / or the detection of post translational modifications of proteins.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

The application provides a WNT5B-promoting tooth differentiation biological product and an application thereof. Compared with the prior art, the application has the following advantages: the patent integrates human embryonic tooth germ spatial transcriptome sequencing and epithelial cell and interstitial cell extracellular protein group sequencing data, screens signal molecules WNT5B and CTNNB1 secreted in the development process of tooth germ epithelium, and confirms the important role of WNT5B protein in tooth germ development for the first time. Compared with other WNT family members, WNT5B has stronger tooth-forming ability of promoting tooth-derived stem cells; the effective concentration of WNT5B is low, and 10 ng / ml is the optimal concentration of WNT5B for promoting tooth-derived stem cells to form teeth; and the role of WNT5B and CTNNB1 in extracellular secretion of tooth germ epithelial cells and the synergistic effect of WNT5B and CTNNB1 in promoting tooth differentiation of tooth germ mesenchymal cells are further determined, that is, the combined low-concentration use effect of the two is better than the single high-concentration use.

To provide bifunctional compounds that can be used to promote or enhance the degradation of certain circulating proteins.SOLUTION: What is claimed is: A compound comprising Formula (I): [Ab] k' - [CON] h - [Linker] I - [CON] h' - [CRBM] j' (I) wherein: Ab is an antibody that binds to an extracellular protein; CRBM is a cell receptor binding moiety that binds to at least one receptor on the surface of a degradative cell in a subject; (I) thereby results in endocytosis and degradation of the extracellular protein; each CON is independently a bond or a group that covalently links the Ab to the CRBM, the Ab to the linker, and / or the linker to the CRBM; the linker is a group having a valence ranging from 1 to 15; k ' is an integer ranging from 1 to 15; h is an integer ranging from 0 to 15; I is an integer ranging from 0 to 15; h ' is an integer ranging from 0 to 15; and j is an integer ranging from 1 to 15.SELECTED DRAWING: Figure 1

Provided herein are bifunctional lysosomal targeting degraders that comprise folate or a folate analog configured to bind to folate receptor as a shuttle molecule for lysosome degradation, and a protein binder configured to bind a membrane or extracellular protein of interest. The bifunctional degraders find use, e.g., for selectively targeted degradation of membrane and extracellular proteins via the endosomal / lysosomal pathway. Also provided herein are compositions comprising the bifunctional degraders, as well as methods of using the bifunctional degraders.

Extracellular protein degraders and compositions with improved pharmacokinetic properties are provided that have a potent asialoglycoprotein receptor (ASGPR) Binding Ligand bound to an Extracellular Protein Targeting Ligand for the selective degradation of the Target Extracellular Protein, for example immunoglobulin in vivo to treat disorders mediated by the extracellular protein.

This invention provides extracellular protein degraders and compositions that have an asialoglycoprotein receptor (ASGPR) Binding Ligand bound to an Extracellular Protein Targeting Ligand for the selective degradation of the Target Extracellular Protein for example an immunoglobulin or other extracellular protein in vivo to treat disorders mediated by that protein.

The invention discloses a method for secretory expression of recombinant mechanical structural protein by using escherichia coli and application, and relates to the field of bioengineering. According to the method, signal peptides are introduced to the N end of recombinant mechanical structural proteins, and the signal peptides, namely OmpA, PelB, PhoA, DsbA and STII, capable of guiding different recombinant mechanical structural proteins to secrete and express are screened from six natural signal peptides from escherichia coli and other species. Meanwhile, the yield of extracellular protein is further improved by combining an amino acid tRNA supplement strategy. According to the method, the recombinant escherichia coli strain capable of efficiently secreting and expressing the mechanical structure protein can be obtained, the downstream purification process is simplified, and the method has important application value.

The invention provides a mammal protein efficient expression system based on a prokaryotic two-component signal transduction system (TCS, two-component system) and application of the mammal protein efficient expression system, and the mammal protein efficient expression system comprises the TCS capable of efficiently recruiting transcription factors and a carrier capable of being induced by the TCS to express a target gene. By transfecting mammalian cells with the system, a protein of interest can be produced in the cells or extracellular proteins can be produced in a secreted form. Based on the mammal protein high-efficiency expression system provided by the invention, stable and high-level expression of the target protein can be realized. The invention provides a new method for production of therapeutic proteins and antibodies, provides key technical support for preparation of mammalian protein samples in the research fields of structural biology and the like, and has important scientific significance for development of life science, diagnosis and treatment of diseases and research and development of medicines.

Provided herein are bifunctional lysosomal targeting degraders including a ligand configured to bind to transferrin receptor as a shuttle molecule for lysosome degradation, and a protein-binding moiety configured to bind a membrane or extracellular protein of interest, wherein the bifunctional lysosome targeting degraders can be recycled back out of cells and be catalytic. The bifunctional degraders find use, e.g., for selectively targeted degradation of membrane or extracellular proteins via the endosomal / lysosomal pathway. Also provided herein are compositions comprising the bifunctional degraders, as well as methods of using the bifunctional degraders.

Polypeptides that bind to asialoglycoprotein receptor (ASGPR), IGF-2R domain 11, IGF-2R, and fusion proteins thereof, that can promote extracellular protein degradation are provided, and methods for their use.

The invention belongs to the fields of bioengineering and animal nutrition, and relates to recombinant lactobacillus plantarum for secretory expression of chicken IGF-1 (Insulin-like Growth Factor-1) as well as a construction method and application thereof. The recombinant lactobacillus plantarum takes lactobacillus plantarum as a host, the host contains a recombinant expression vector, and the recombinant expression vector contains an SlpA promoter, a lactobacillus plantarum extracellular protein Lp2007 signal peptide gene and a chicken IGF-1 gene; the sequence of the chicken IGF-1 gene is as shown in SEQ ID NO: 1, the sequence of the SlpA promoter is as shown in SEQ ID NO: 2, and the sequence of the lactobacillus plantarum extracellular protein Lp2007 signal peptide is as shown in SEQ ID NO: 3. The recombinant lactobacillus plantarum can secrete IGF-1 outside cells of the recombinant lactobacillus plantarum. When the recombinant lactobacillus plantarum for secreting and expressing the chicken IGF-1 is used for feeding chickens, the growth speed of the chickens can be remarkably increased, cost reduction and efficiency improvement of the poultry raising industry are realized, and the recombinant lactobacillus plantarum has important economic and social values.

The present invention belongs to the field of biological fermentation technology, and specifically relates to Kluyveromyces lactis and its use. The present invention provides a Kluyveromyces lactis, which is deposited in the China Center for Type Culture Collection, and its deposit number is CCTCC NO: M 20241460. The Kluyveromyces lactis significantly improves the problem of poor extracellular protein secretion and is a good host choice for protein expression.

The present disclosure relates to novel nucleic acids for immune cell adapters, comprising a ribonucleic acid of formula I: R1-SP-R2-R3-R4-R5 (I) wherein R1 is a 5'non-transcriptional region (UTR); sP encodes a signal peptide; r2 encodes a first single chain variable fragment (scFv) that binds to a first extracellular protein or a heavy chain variable region (VH only) that binds to a first extracellular protein; r3 encodes a second scFv that binds to a second extracellular protein; r4 encodes a half-life extender; r < 5 > is 3 'UTR wherein R < 1 > to R < 5 > are in the 5' to 3 'direction for use in the treatment of cancer, including but not limited to hematological cancers, including multiple myeloma.

This invention discloses a method for preparing acellular dermal matrix, belonging to the field of tissue engineering technology. The preparation method includes: selecting pelts from traceable animals aged one to three months, purifying them through steps such as soaking, fleshing, defatting, hair removal, liming, deliming, and softening in a tanning process to obtain acellular dermal matrix. The acellular dermal matrix obtained by this invention removes antigenic components such as cells, hair follicles, and extracellular proteins during the preparation process, minimizing its immunogenicity while preserving the collagen fiber structure in its extracellular matrix. Simultaneously, the preparation method of this invention moderately loosens the collagen fiber bundles. This process not only effectively alleviates the entanglement between fiber bundles but also successfully increases the exposure of hydrophilic groups, giving the acellular dermal matrix excellent hydrophilicity, which is more conducive to cell proliferation and migration.

This invention relates to the field of microbial technology, specifically to a method for the production and extraction of N-acetylmuroic acid-L-alanine amidase via microbial fermentation. The method for preparing N-acetylmuroic acid-L-alanine amidase provided by this invention utilizes *Clostridium beijerinckii* XH0906 for fermentation to produce N-acetylmuroic acid-L-alanine amidase. As the main extracellular protein of this bacterium, N-acetylmuroic acid-L-alanine amidase has a high yield and is beneficial for subsequent separation and purification. This method has advantages such as simplicity, efficiency, and low cost, providing an effective method for the preparation of N-acetylmuroic acid-L-alanine amidase, and has good application prospects in the fields of microbial food, biomedicine, and antibacterial applications.

This invention discloses a protease-responsive GPCR receptor and its applications. The protease-responsive GPCR receptor of this invention comprises an extracellular protease recognition domain, a transmembrane domain, and an intracellular signaling domain; the extracellular protease recognition domain contains a peptide hydrolyzed by the protease; the intracellular signaling domain contains an HCV enzyme hydrolyzed peptide, a nuclear localization sequence (NLS), a Cas protease, and a coupled nuclear transcription factor. This protease-responsive GPCR receptor can induce endogenous dCas9 assembly into the nucleus and activate the chimeric antigen receptor signaling pathway in natural killer cells in response to extracellular proteases, providing a new solution for developing novel precision immunotherapy.

The present invention provides a gene for increasing glucose oxidase (GOD) production in Pichia pastoris and its use. Specifically, the present invention provides a method for constructing a glucose oxidase (GOD)-producing strain, wherein the activity of a vesicle secretion-promoting factor is enhanced or a vesicle secretion-promoting factor is introduced into the starting strain. Experiments have confirmed that co-expression of the VAMP4 gene, which is involved in the vesicle transport pathway from the Golgi apparatus to the plasma membrane, significantly increases extracellular GOD production in Pichia pastoris.

The application discloses a method for treating antibiotic wastewater by using high-concentration ammonia-nitrogen wastewater and application thereof, and belongs to the technical field of purification treatment. The method is characterized in that: microalgae cells cultured to the logarithmic phase are inoculated into sterilized high-concentration ammonia-nitrogen wastewater for culture, and then the microalgae cells are used for treating antibiotic wastewater. The method provided by the application has remarkable economic benefits, and the microalgae are widely distributed in nature, so that no additional carbon source is needed, and the cost is low. Moreover, more extracellular proteins are secreted by using high-concentration ammonia-nitrogen, and the microalgae remove the antibiotics while achieving the purpose of treating ammonia-nitrogen.