46 results about "Extracellular proteins" patented technology

The invention discloses a lysosome targeted chimera based on Sortilin and application thereof. The lysosome targeted chimera comprises a ligand molecule of a lysosome targeted receptor and a ligand molecule of a targeted protein to be degraded, wherein the ligand molecule and the ligand molecule are connected through a linker; the lysosome targeting receptor is Sortilin, and the ligand molecule of the lysosome targeting receptor is a polypeptide targeting the Sortilin; the protein to be degraded is membrane protein or extracellular protein. Furthermore, the polypeptide of the targeted Sortilin is connected with an enzyme response sequence and an integrin receptor targeting sequence. According to the invention, the Sortilin-combined ligand neurotensin is functionally coupled with the neutralizing antibody or polypeptide of overexpression protein in targeted tumor or inflammatory diseases, and the Sortilin-combined ligand neurotensin has obvious treatment gain in malignant solid tumor and psoriasis models. The discovery promotes the transformation potential of the lysosome participating in the biological agent, and provides a dual strategy for realizing accurate degradation through receptor recruitment and microenvironment induction.

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

The application provides a WNT5B-promoting tooth differentiation biological product and an application thereof. Compared with the prior art, the application has the following advantages: the patent integrates human embryonic tooth germ spatial transcriptome sequencing and epithelial cell and interstitial cell extracellular protein group sequencing data, screens signal molecules WNT5B and CTNNB1 secreted in the development process of tooth germ epithelium, and confirms the important role of WNT5B protein in tooth germ development for the first time. Compared with other WNT family members, WNT5B has stronger tooth-forming ability of promoting tooth-derived stem cells; the effective concentration of WNT5B is low, and 10 ng / ml is the optimal concentration of WNT5B for promoting tooth-derived stem cells to form teeth; and the role of WNT5B and CTNNB1 in extracellular secretion of tooth germ epithelial cells and the synergistic effect of WNT5B and CTNNB1 in promoting tooth differentiation of tooth germ mesenchymal cells are further determined, that is, the combined low-concentration use effect of the two is better than the single high-concentration use.

To provide bifunctional compounds that can be used to promote or enhance the degradation of certain circulating proteins.SOLUTION: What is claimed is: A compound comprising Formula (I): [Ab] k' - [CON] h - [Linker] I - [CON] h' - [CRBM] j' (I) wherein: Ab is an antibody that binds to an extracellular protein; CRBM is a cell receptor binding moiety that binds to at least one receptor on the surface of a degradative cell in a subject; (I) thereby results in endocytosis and degradation of the extracellular protein; each CON is independently a bond or a group that covalently links the Ab to the CRBM, the Ab to the linker, and / or the linker to the CRBM; the linker is a group having a valence ranging from 1 to 15; k ' is an integer ranging from 1 to 15; h is an integer ranging from 0 to 15; I is an integer ranging from 0 to 15; h ' is an integer ranging from 0 to 15; and j is an integer ranging from 1 to 15.SELECTED DRAWING: Figure 1

Extracellular protein degraders and compositions with improved pharmacokinetic properties are provided that have a potent asialoglycoprotein receptor (ASGPR) Binding Ligand bound to an Extracellular Protein Targeting Ligand for the selective degradation of the Target Extracellular Protein, for example immunoglobulin in vivo to treat disorders mediated by the extracellular protein.

This invention provides extracellular protein degraders and compositions that have an asialoglycoprotein receptor (ASGPR) Binding Ligand bound to an Extracellular Protein Targeting Ligand for the selective degradation of the Target Extracellular Protein for example an immunoglobulin or other extracellular protein in vivo to treat disorders mediated by that protein.

The invention discloses a method for secretory expression of recombinant mechanical structural protein by using escherichia coli and application, and relates to the field of bioengineering. According to the method, signal peptides are introduced to the N end of recombinant mechanical structural proteins, and the signal peptides, namely OmpA, PelB, PhoA, DsbA and STII, capable of guiding different recombinant mechanical structural proteins to secrete and express are screened from six natural signal peptides from escherichia coli and other species. Meanwhile, the yield of extracellular protein is further improved by combining an amino acid tRNA supplement strategy. According to the method, the recombinant escherichia coli strain capable of efficiently secreting and expressing the mechanical structure protein can be obtained, the downstream purification process is simplified, and the method has important application value.

The invention provides a mammal protein efficient expression system based on a prokaryotic two-component signal transduction system (TCS, two-component system) and application of the mammal protein efficient expression system, and the mammal protein efficient expression system comprises the TCS capable of efficiently recruiting transcription factors and a carrier capable of being induced by the TCS to express a target gene. By transfecting mammalian cells with the system, a protein of interest can be produced in the cells or extracellular proteins can be produced in a secreted form. Based on the mammal protein high-efficiency expression system provided by the invention, stable and high-level expression of the target protein can be realized. The invention provides a new method for production of therapeutic proteins and antibodies, provides key technical support for preparation of mammalian protein samples in the research fields of structural biology and the like, and has important scientific significance for development of life science, diagnosis and treatment of diseases and research and development of medicines.

The invention belongs to the fields of bioengineering and animal nutrition, and relates to recombinant lactobacillus plantarum for secretory expression of chicken IGF-1 (Insulin-like Growth Factor-1) as well as a construction method and application thereof. The recombinant lactobacillus plantarum takes lactobacillus plantarum as a host, the host contains a recombinant expression vector, and the recombinant expression vector contains an SlpA promoter, a lactobacillus plantarum extracellular protein Lp2007 signal peptide gene and a chicken IGF-1 gene; the sequence of the chicken IGF-1 gene is as shown in SEQ ID NO: 1, the sequence of the SlpA promoter is as shown in SEQ ID NO: 2, and the sequence of the lactobacillus plantarum extracellular protein Lp2007 signal peptide is as shown in SEQ ID NO: 3. The recombinant lactobacillus plantarum can secrete IGF-1 outside cells of the recombinant lactobacillus plantarum. When the recombinant lactobacillus plantarum for secreting and expressing the chicken IGF-1 is used for feeding chickens, the growth speed of the chickens can be remarkably increased, cost reduction and efficiency improvement of the poultry raising industry are realized, and the recombinant lactobacillus plantarum has important economic and social values.

The present disclosure relates to novel nucleic acids for immune cell adapters, comprising a ribonucleic acid of formula I: R1-SP-R2-R3-R4-R5 (I) wherein R1 is a 5'non-transcriptional region (UTR); sP encodes a signal peptide; r2 encodes a first single chain variable fragment (scFv) that binds to a first extracellular protein or a heavy chain variable region (VH only) that binds to a first extracellular protein; r3 encodes a second scFv that binds to a second extracellular protein; r4 encodes a half-life extender; r < 5 > is 3 'UTR wherein R < 1 > to R < 5 > are in the 5' to 3 'direction for use in the treatment of cancer, including but not limited to hematological cancers, including multiple myeloma.

This invention discloses a method for preparing acellular dermal matrix, belonging to the field of tissue engineering technology. The preparation method includes: selecting pelts from traceable animals aged one to three months, purifying them through steps such as soaking, fleshing, defatting, hair removal, liming, deliming, and softening in a tanning process to obtain acellular dermal matrix. The acellular dermal matrix obtained by this invention removes antigenic components such as cells, hair follicles, and extracellular proteins during the preparation process, minimizing its immunogenicity while preserving the collagen fiber structure in its extracellular matrix. Simultaneously, the preparation method of this invention moderately loosens the collagen fiber bundles. This process not only effectively alleviates the entanglement between fiber bundles but also successfully increases the exposure of hydrophilic groups, giving the acellular dermal matrix excellent hydrophilicity, which is more conducive to cell proliferation and migration.

This invention relates to the field of microbial technology, specifically to a method for the production and extraction of N-acetylmuroic acid-L-alanine amidase via microbial fermentation. The method for preparing N-acetylmuroic acid-L-alanine amidase provided by this invention utilizes *Clostridium beijerinckii* XH0906 for fermentation to produce N-acetylmuroic acid-L-alanine amidase. As the main extracellular protein of this bacterium, N-acetylmuroic acid-L-alanine amidase has a high yield and is beneficial for subsequent separation and purification. This method has advantages such as simplicity, efficiency, and low cost, providing an effective method for the preparation of N-acetylmuroic acid-L-alanine amidase, and has good application prospects in the fields of microbial food, biomedicine, and antibacterial applications.

This invention discloses a protease-responsive GPCR receptor and its applications. The protease-responsive GPCR receptor of this invention comprises an extracellular protease recognition domain, a transmembrane domain, and an intracellular signaling domain; the extracellular protease recognition domain contains a peptide hydrolyzed by the protease; the intracellular signaling domain contains an HCV enzyme hydrolyzed peptide, a nuclear localization sequence (NLS), a Cas protease, and a coupled nuclear transcription factor. This protease-responsive GPCR receptor can induce endogenous dCas9 assembly into the nucleus and activate the chimeric antigen receptor signaling pathway in natural killer cells in response to extracellular proteases, providing a new solution for developing novel precision immunotherapy.

The present invention provides a gene for increasing glucose oxidase (GOD) production in Pichia pastoris and its use. Specifically, the present invention provides a method for constructing a glucose oxidase (GOD)-producing strain, wherein the activity of a vesicle secretion-promoting factor is enhanced or a vesicle secretion-promoting factor is introduced into the starting strain. Experiments have confirmed that co-expression of the VAMP4 gene, which is involved in the vesicle transport pathway from the Golgi apparatus to the plasma membrane, significantly increases extracellular GOD production in Pichia pastoris.

The application discloses a method for treating antibiotic wastewater by using high-concentration ammonia-nitrogen wastewater and application thereof, and belongs to the technical field of purification treatment. The method is characterized in that: microalgae cells cultured to the logarithmic phase are inoculated into sterilized high-concentration ammonia-nitrogen wastewater for culture, and then the microalgae cells are used for treating antibiotic wastewater. The method provided by the application has remarkable economic benefits, and the microalgae are widely distributed in nature, so that no additional carbon source is needed, and the cost is low. Moreover, more extracellular proteins are secreted by using high-concentration ammonia-nitrogen, and the microalgae remove the antibiotics while achieving the purpose of treating ammonia-nitrogen.

The present disclosure relates, inter alia, to compositions and methods, including chimeric proteins, and nucleic acids encoding the chimeric proteins having a first domain comprising an extracellular domain of a first transmembrane protein, a first secreted protein, or a first membrane-anchored extracellular protein and a second domain comprising an extracellular domain of a second transmembrane protein, a second secreted protein, or a second membrane-anchored extracellular protein, in which either or both of the first domain and the second domain decreases self-directed immune system activity when bound to its ligand / receptor. Accordingly, the present disclosure find use in the treatment of autoimmune diseases, and particularly, inflammatory bowel diseases.

This invention discloses a method for efficiently extracting and enriching sludge-based antimicrobial peptides from activated sludge, belonging to the field of activated sludge resource recycling and utilization. The invention removes impurities from the activated sludge by releasing intracellular and extracellular proteins through low-temperature hot alkaline hydrolysis, followed by enzymatic hydrolysis with proteases to obtain a crude extract containing peptides of different molecular weights. Further purification involves primary purification to remove impurities and secondary purification to separate amino acids and peptides from the organic matter mixture, yielding purified antimicrobial peptide products of different molecular weights. Antimicrobial activity is assessed using an inhibition zone assay, resulting in a highly effective antimicrobial sludge-based antimicrobial peptide molecular template. This template is used to prepare a magnetic molecularly imprinted polymer (Fe3O4@MIPs). Fe3O4@MIPs are then used to adsorb and enrich the highly effective antimicrobial sludge-based antimicrobial peptides in the crude extract. After elution with an eluent, highly effective antimicrobial sludge-based antimicrobial peptides are obtained.

The invention discloses lactobacillus gasseri BSJM25L011, lactobacillus gasseri extracellular protein with an oil control effect as well as a preparation method and application of the lactobacillus gasseri extracellular protein. The lactobacillus gasseri has the taxonomic name of Lactobacillus gasseri, is preserved in the China General Microbiological Culture Collection Center on June 16, 2025, has the preservation number of CGMCC No.34898, has the preservation address of No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and has the postcode of 100101, and the lactobacillus gasseri extracellular protein with the oil control effect is obtained by fermenting and culturing the lactobacillus gasseri strain BSJM25L011. The lactobacillus gasseri BSJM25L011 can generate a novel, mild and efficient natural biological oil control active component, and the lactobacillus gasseri extracellular protein disclosed by the invention has an excellent oil control effect, can remarkably regulate skin sebum secretion, can be widely applied to the field of oil control skin external preparations, expands the application field of the lactobacillus gasseri, and has a good application prospect. And the use safety is high.

Compounds and compositions that have a Mannose 6-Phosphate Receptor Ligand bound to an Extracellular Protein Binding Ligand are provided for the selective degradation of a target extracellular protein in vivo to treat disorders mediated by the extracellular protein.

The present application belongs to the field of agricultural biotechnology, and through a large number of sequence analysis and tests, a short peptide with a function of antagonizing fungi in an extracellular protein Atp2 beta subunit protein of Rhodopseudomonas palustris is found. Tests show that the short peptide with the function of antagonizing fungi has low production cost, is non-toxic and environmentally friendly, can significantly inhibit the formation of appressorium of rice blast fungus and the pathogenicity on the host, and has potential broad-spectrum antagonistic function activity.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

The invention discloses a sludge dewatering agent based on regulation and control of a hydrogen bond network structure as well as a preparation method and application thereof, relates to the field of environmental engineering, and aims to solve the problems of increase of subsequent treatment difficulty, long time consumption and high cost caused by excessive addition of an existing chemical conditioning agent in a sludge dewatering process. The sludge dewatering agent comprises ferric chloride hexahydrate and choline chloride. Mixing ferric trichloride hexahydrate and choline chloride, heating and stirring until a homogeneous phase is formed, so as to obtain the sludge dewatering agent. And a complex hydrogen bond network is formed between the ferric chloride hexahydrate and the choline chloride, so that the ferric chloride hexahydrate and the choline chloride have relatively strong hydrogen bond coordination capability, namely, the capability of reconstructing the hydrogen bond network. According to the method, the EPS structure is damaged by changing the hydrogen bond network structure in the extracellular protein of the sludge, so that bound water is released, and the solid-liquid separation effect of the sludge is improved. The device is used for sludge dewatering.

Provided herein is a bifunctional lysosomal targeted degradation agent comprising: a peptide ligand that binds to an RGD binding integrin as a shuttle molecule for lysosomal degradation; and a protein binding agent that binds to a membrane of interest or an extracellular protein. The bifunctional degradation agents are useful, for example, for selective targeted degradation of membranes and extracellular proteins through endosomal / lysosomal pathways. Also provided herein are compositions comprising the bifunctional degradation agents and methods of using the bifunctional degradation agents.

Compounds and compositions are described that have an asialoglycoprotein receptor (ASGPR) binding ligand conjugated to an extracellular protein binding ligand that selectively degrades target extracellular proteins in vivo to treat disorders mediated by the extracellular proteins.

The disclosure describes compounds of Formula Ia, which in non-limiting aspects contain an asialoglycoprotein receptor (ASGPR) binding moiety and an anti-β1AR binding moiety. Compounds of Formula Ia are useful in preventing, treating, and / or ameliorating heart failure in a subject when administered in therapeutically effective amounts.

The invention discloses a method for improving extracellular protein yield and hemicellulase activity of filamentous fungi, and belongs to the field of gene engineering. According to the method, production of extracellular protein and hemicellulase of filamentous fungi is improved by knocking out or mutating a part of basic groups of a transcription factor or weakening expression of the transcription factor, and a transcription factor coding gene is NCU04001. The recombinant bacteria obtained by knocking out or mutating partial bases or weakening filamentous fungus transcription factor gene expression can be applied to degrading cellulose or improving cellulase expression or expression of other extracellular proteins.

The invention relates to the technical field of biology, in particular to a trichoderma reesei engineering strain for high yield of cellulase and xylanase and a construction method and application of the trichoderma reesei engineering strain. The regulatory gene Tr100508 is knocked out from the gene knockout engineering strain, and the filter paper enzyme activity, the endo-cellulase activity, the xylanase enzyme activity and the extracellular protein content of the trichoderma reesei engineering strain are remarkably improved, so that the trichoderma reesei engineering strain shows good industrial development and application prospects.