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Protein derived from Trichoderma reesei and gene application thereof

A technology of Trichoderma reesei and genes, applied in the direction of microorganism-based methods, using vectors to introduce foreign genetic material, cells modified by introducing foreign genetic material, etc., can solve the problem of regulatory factors not being discovered

Inactive Publication Date: 2012-11-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the complexity of the expression regulation of Trichoderma reesei cellulase, there should be many key regulatory factors in the process of its transcriptional regulation that have not yet been discovered

Method used

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  • Protein derived from Trichoderma reesei and gene application thereof
  • Protein derived from Trichoderma reesei and gene application thereof
  • Protein derived from Trichoderma reesei and gene application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1, Knockout of Trichoderma reesei Trfog1 Gene Construction of Recombinant Trichoderma reesei with Increased Extracellular Protein Secretion

[0063] In this example, the Trfog1 gene was knocked out by homologous recombination by introducing the DNA fragment (knockout component) shown in sequence 4 in the sequence listing into the starting strain of Trichoderma reesei - Trichoderma reesei strain TU6Δtku70 Extracellular protein secretion was significantly increased, especially the Trfog1 gene knockout strain (recombinant Trichoderma reesei) with significantly increased cellulase secretion.

[0064] 1. PCR amplification of Trfog1 gene (including homology arms on both sides) and pyr4 gene expression cassette

[0065] With Trichoderma reesei (Trichoderma reesei) strain QM9414 (ATCC 26921) genomic DNA as a template, primers Fog1-F, Fog1-R amplify the Trfog1 gene fragment comprising the homology arms on both sides of Trfog1 (upstream homology arm 1395bp, Trfog1 gene 1...

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Abstract

The invention discloses a protein derived from Trichoderma reesei and application the gene of the protein. The application is the application of the Trfogl to controlling the extracellular protein secretion amount of the Trichoderma reesei, and the Trfogl is the protein shown by an amino acid sequence such as a sequence 2 in a sequence table. According to the invention, a technical base is provided for constructing Trichoderma reesei host bacteria and engineering bacteria with an increased extracellular protein secretion amount.

Description

technical field [0001] The present invention relates to the use of a protein derived from Trichoderma reesei and its gene, in particular to the application of Trfog1 derived from Trichoderma reesei in regulating the secretion of extracellular protein of Trichoderma reesei. Background technique [0002] The filamentous fungus Trichoderma reesei (Trichoderma reesei) is a mesophilic saprophytic fungus, which is widely used in the production of cellulose and hemicellulase for food, feed, textile, pulp and paper industries. At present, it has become an important model strain for the study of cellulose degradation. Trichoderma reesei is an FDA-certified safe production strain with strong protein secretion ability and a glycosylation system similar to higher mammals, so Trichoderma reesei is also an ideal host for recombinant protein expression. In order to further increase the production of cellulase hemicellulase of Trichoderma reesei and at the same time increase the secretion ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N15/09C12N1/20C12N15/11C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C07K14/37C12R1/885
Inventor 董志扬秦丽娜
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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