Trichoderma reesei recombinant strain capable of highly producing cellulase and application thereof

Abstract

The invention discloses a trichoderma reesei recombinant strain capable of highly producing cellulase and application thereof. Vlbltr which grows an incompatible repression protein gene is transformed into trichoderma reesei Rut-C30, so as to obtain the trichoderma reesei Vib1 (CCMCC No.13578). Compared with the strain Vib1 and the trichoderma reesei Rut-C30, the trichoderma reesei recombinant strain has the advantages that the excretion of extracellular protein and the production of cellulase are obviously improved; compared with the trichoderma reesei Rut-C30, the activity of cellulase in the Vib1 in cellulose and bran culture mediums is increased by about 200%, and reaches 3.3U / ml; the enzyme activities of cellobiohydrolase, endo-cellulase and beta-glucosidase are respectively improved; the excretion amount of the extracellular protein in the Vib1 strain is equal to 2.19 times of the excretion amount of trichoderma reesei Rut-30; proved by the results of hydrolysis reaction of pretreated corn straw, compared with the Rut-30 strain, the output of glucose in the Vib1 strain cellulase hydrolyte is increased by 20%.

Description

technical field

[0001] The invention belongs to the field of microbial biotechnology; in particular, it relates to a recombinant bacterial strain of Trichoderma reesei with high cellulase production and application thereof. Background technique

[0002] With the continuous consumption of fossil energy, the development of renewable biomass energy has attracted widespread attention at home and abroad. Lignocellulosic biomass resources are abundant and renewable, and are potential alternative energy sources for fossil energy. Cellulase and hemicellulase can be used to hydrolyze them into glucose and xylose, and further ferment them to produce renewable bioenergy and Wide range of bio-based chemicals. However, in the bioconversion process of lignocellulosic biomass, the high production cost of cellulase is one of the main bottlenecks restricting its industrial application. Therefore, it is very important to improve the production efficiency of strain cellulase and reduce the p...

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