407 results about "Beta-Glucosidases" patented technology

The invention relates to a natural tea essence prepared by using tea and a preparation method thereof. The tea is taken as a raw material; high-purity natural tea essences with different tea aroma characteristics are prepared through compound enzymolysis, releasing, steam distillation under reduced pressure, cold trap recycling, low temperature freezing concentration, centrifugal oil-water separation, and trace moisture adsorption by using anhydrous sodium sulfate; and the compound enzymolysis and the releasing are realized by adding pectinase, cellulase or pectinase and cellulose complex enzyme, and beta-glucosidase. In the preparation method, a pectinase and cellulose complex enzyme method is adopted for enzymolysis to facilitate cracking cell walls of the tea so as to facilitate liberation and separation of aroma components from the tea, and improve the yield of the essence; therefore, the method is a method for industrially producing the natural tea essence which is energy-saving, low in cost, environmentally friendly and high in efficiency.

The invention discloses a making method of heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase, which comprises the following steps: adopting agricultural waste as carbon source; fermenting Paecilomyces thermophila J18 at 40-60 deg.c to obtain the ferment liquid with heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase; purifying; obtaining electrophoretic grade product.

The invention belongs to the biotechnology field and relates to a preparation method of genipin. The method comprises a preparation process of immobilized enzyme, a transformation process of gardenoside and a separation and purification process of genipin and is characterized by fermenting the strains for producing beta-glucosidase, collecting the fermentation liquor after reaching the enzyme producing peak, co-immobilizing enzyme and cells or enzyme and hyphae by combining embedding with crosslinking to prepare the immobilized enzyme, utilizing a packed-bed or stirred reactor to carry out catalyzed hydrolysis on the gardenoside and obtaining genipin after purification. The invention dispenses with separation and purification of the fermentation liquor, the immobilized enzyme obtained by preparation has high activity still keeping over 50% after operation for 960h and produces less pollution to the environment, genipin has high yield and the method has obvious advantages compared with other methods.

The invention provides a production method of a natural blue pigment, which comprises genipin prepared by reaction of beta-glucosidase catalyzing gargenoside, and natural gardenia blue pigment prepared by reaction between the genipin and amino acid in a proper condition. The production method is characterized in that metalloenzyme accelerator is added in the reaction of beta-glucosidase catalyzing gargenoside. Because the metalloenzyme accelerator is added in the reaction, the speed of enzymatic reaction can be accelerated and the reaction time is shortened, so that the production cost is saved, which is helpful for industrialized production. Meanwhile, metal ions added are all the trace elements that the human body needs, so the prepared pigment not only does not have the potential harm that the artificial pigment has, but oppositely has good health function, therefore the pigment can be applied in various fields like food industry.

Disclosed is a method for preparing tobacco extractive by biological enzyme, which comprises using minced tobacco, low grade tobacco leaves as raw material, charging pectolase, cellulose and hemicellulase, or the complex enzyme of enzymes selected from above for enzymolysis, thus hydrolyzing macromolecules of the pectolase or cellulose into micromolecular substances helpful for enhancing and improving scent for tobacco extracts, a second enzymolysis can also be carried out by charging aliphatic acid enzyme, cigarette alkali degradation enzyme or beta-glucosidase.

Isolation plating medium of a first color for the identification of Listeria sp. and Listeria monocytogenes and Listeria ivanovii containing a first chromogenic substrate that responds to phosphatidylinositol-specific phospholipase C enzymes to release precipitate of a second color into the medium and a second chromogenic substrate that responds to beta-glucosidase enzymes to release precipitate of a third color into the medium, and said first, second and third colors contrasting with each other.

The invention discloses a fermentation tea juice and a preparation method thereof. The preparation method comprises the following steps: taking tea leaves, crushing, adding water to extract, adding beta-glucosidase to the obtained extract liquid in order to carry out enzymatic hydrolysis, and filtering the above obtained enzymatic hydrolysis liquid to obtain a tea juice; concentrating the tea juice until the concentration of tea polyphenol is 10-120g / L, and adding a carbon source and a plant source oligopeptide into the tea juice in order to disinfect; inoculating Leuconostoc mesenteroides into the tea juice after the disinfection in order to ferment, and inoculating yeast and lactic acid bacteria into the above obtained fermentation liquid when the pH value of the fermentation liquid is 4.0-4.5 in order to carry out mixed fermentation; and collecting the obtained fermentation liquid, and post-processing the fermentation liquid to prepare the fermentation tea juice. In the invention, the glucoside modification of catechin is carried out in the fermentation process, so the flavor quality and the bioavailability of the above tea juice fermentation beverage are improved, the stability of functional components is improved, and the overall coordination effect of bioactive components in the tea leaves are comprehensively performed.

The invention discloses a saccharifying method of xylon cellulose through ultrasonic coordinated modified cellulose enzyme, which comprises the following steps: grinding and predisposoing xylon in the raw material through alkaline; reacting activated methoxy carbowax and cellulose enzyme in the citrate-sodium citrate buffer solution to obtain modified cellulose enzyme; blending modified cellulose enzyme, beta-glucosidase, amylase and pectase to obtain composite liquid; adding composite enzyme into predisposed raw material according to corresponding proportion; proceeding enzyme catalytic reaction through ultrasound; filtering; decompressing; evaporating; obtaining condensed sugar liquid.

The invention provides a preparation method and an application of a discarded tobacco leaf fermentation extract. Discarded tobacco leaves are fermented with beta-glucosidase, a tobacco biotransformation liquid containing various increased small-molecular aroma substances is obtained through enzymatic curing, then amino acid and reducing sugar in certain proportion are added for a high-temperature Maillard reaction under an alkaline condition, a natural tobacco Maillard reaction liquid is obtained and subjected to pumping filtration, a filtrate and filter residues are obtained, the filtrate is concentrated and mixed with propylene glycol and the like, further separation and purification are performed through molecular distillation, and light components are collected and taken as the discarded tobacco leaf fermentation extract. Reconstituted tobacco substrates with low irritation, low miscellaneous gas, low nicotine content and low harm are prepared from the filter residues with a paper-making process, the quality of the thin discarded tobacco leaf substrates is improved, the use effect of the discarded tobacco leaves on cigarette products is improved, the adding quantity of the extract is increased, the extract can be applied to an atomization filler material for novel carbon heating tobacco, and the quality of the atomization filler material is improved.

The invention discloses a method for preparing gentio-oligosaccharide by using immobilized beta-glucosidase. The method for preparing the gentio-oligosaccharide by using the immobilized beta-glucosidase comprises the following steps of: 1) preparing beta-glucosidase through solid-state fermentation of Aspergillus niger; 2) immobilizing the beta-glucosidase by a crosslinking-embedding method; and 3) producing the gentio-oligosaccharide by transforming glucose by using the immobilized beta-glucosidase. The recovery rate of enzyme activity of an immobilized enzyme prepared by the method is up to 60 percent, and the conversion rate of a substrate, namely the glucose converted by the immobilized enzyme is 45 percent; and the stability and pH tolerance of the immobilized enzyme are remarkably improved, the mechanical strength is high, the immobilized enzyme can be continuously and repeatedly used for 4 to 6 bathes, and the enzyme activity is kept unchanged. The utilization rate of the gentio-oligosaccharide produced by an enzyme method is improved, the production cost and difficulty in separation and purification of the gentio-oligosaccharide are reduced, and the method is suitable for large-scale continuous production and has a great significance for improving the technical level of the production of the gentio-oligosaccharide in China.

The invention discloses a penicillium deumbens engineered strain containing over-expressed beta-glucosidase, which contains over-expressed beta-glucosidase genes, is named penicillium deumbens Gi31-2 and is preserved in China Center for Type Culture Collection on May 7, 2010 with a preservation number of CCTCC M 2010111. The invention also discloses the application of the strain in production of the beta-glucosidase and degradation of cellulose. The highest activity of the beta-glucosidase of the engineered strain of the invention reaches 3.47 IU / ml and is 2.90 times as high as that of an original host strain, while highest filter paper activity is 3.32 IU / ml and is 1.23 times as high as that of the original host strain. Therefore, the strain is widely applied to the production of the beta-glucosidase.

The invention discloses a method for producing soybean isoflavone aglycone through microbe's enzyme method, and is culturing Aspergillus oryzae with soybean isoflavone powder as raw materials for fermentation to produce beta-glucosidase, and converting soybean isoflavone to soybean isoflavone aglycone. The method is characterized in comprising the following preparation steps: the said raw materials are 2-90% soybean isoflavone powder, the said strain is Aspergillus oryzae 3042, preparing seed culture liquid with the strain, preparing solid fermentation medium, fermenting to obtain fermenting product containing beta-glucosidase, adding soybean isoflavone powder for preparing enzyme converting solution, centrifuging for separation to obtain deposit, drying at low temperature to obtain soybean isoflavone aglycone. The invention avoids the restriction of the raw materials purity, converting rata can reach to over 90%. The invention has the advantages of short procedure, low cost, high bioavailability.

The invention relates to a novel beta glucosidase / xylosidase difunctional cellulose degradation enzyme RuGBGX2 as well as a coding gene and application thereof. The coding sequence of amino acid of the RuGBGX2 contains 18-755th sites of an SEQ ID NO 2 sequence. The RuGBGX2 is sourced from the rumen microorganism of yak from China, a novel coding gene of the beta glucosidase / xylosidase difunctional cellulose degradation enzyme RuGBGX2 is obtained by function screening and sequencing analysis on a rumen metagenome cosmid library and a subclone library. The beta glucosidase / xylosidase difunctional cellulose degradation enzyme provided by the invention can be widely applied to the degradation of cellulose and the fields such as cellulose biotransformation, chemical industry, spinning, foods, bioenergy, feed additives, medical industry and the like. By utilizing the difunctional enzyme RuGBGX2 to degrade wood fiber, the varieties of added enzymes can be reduced, and an enzymolysis process can be simplified.

The present invention discloses beta-glucosidase, coding gene, a vector, engineering bacteria and an application of the beta-glucosidase in preparing resveratrol through hydrolysis of polydatin. The beta-glucosidase gene shares 70-100% sequence identity with the polynucleotide sequence represented by the SEQ ID NO:2. The beta-glucosidase coded by the beta-glucosidase gene shares 95-100% sequence identity with the amino acid sequence represented by the SEQ ID NO.1. With the beta-glucosidase provided by the present invention, the resveratrol can be prepared through hydrolyzing the polydatin, the preparation process is environmentally-friendly, the yield is high and the application prospect is broad.

The invention relates to a method for preparing rebaudioside through packed bed enzymatic hydrolysis of stevioside. The method comprises the following steps of: dissolving the stevioside or stevia rebaudiana extract in water or phosphate buffer solution; combining the parts containing rebaudioside of the solution through an immobilization beta-glucosidase packed column; and concentrating and recrystallizing to obtain the rebaudioside. The preparation of rebaudioside takes 5 days in the prior art, and the transformation activity of the beta-glucosidase repeatedly used for 4 times is only 57.72% of the original activity. Through the invention, the preparation of rebaudioside using the immobilization beta-glucosidase only takes 12 hours, and the transformation rate of stevioside after 5 times of use still exceeds 84%. Thus, the method provided by the invention is short in production period, high in production efficiency and easy to realize continuous production, and has very broad application prospects.

The invention relates to a centella asiatica triterpenic acid single-glucopyranoside composition which is composed of ursane madecassic acid single-glucopyranoside, madecassic acid single-glucopyranoside and oleanane chebuloside II, wherein the mass ratio of ursane madecassic acid single-glucopyranoside to madecassic acid single-glucopyranoside to oleanane chebuloside II is 1:0.5-2:0.1-1, and the sum of the mass percentage content of three components is not less than 50%. The composition takes a centella asiatica extract as a substrate, the centella asiatica extract is fermented and hydrolyzed by microbes of beta-glucosidase or microbes capable of generating beta-glucosidase, and extracted by n-butanol or separated and purified by macroporous adsorption resin. The invention also provides a quantitative analysis method which is a HPLC quantitative analysis for three components by adding a proper amount of mobile phase of beta-cyclodextrin. Experimental research of pharmacodynamics proves that the composition has substantial activity for inhibiting tumor cells and fibroblast, the composition can be used for treating tumor and scar hyperplasia.

The invention relates to novel beta-glucosidase, and its coding gene and application. The invention further relates to an expression vector and host cells which contain the coding gene. The invention also relates to a method for using the beta-glucosidase to hydrolyze cellobiose generated in the process of cellulose degradation to glucose. The beta-glucosidase disclosed herein has the characteristics of high temperature resistance and good stability under neutral and alkaline conditions, and can be well applied in industrial production.

The invention discloses a trichoderma viride W2 capable of producing a thermophilic ethanol-resistant beta-glucosidase and an application thereof. The trichoderma viride W2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 23, 2010, and the preservation number of the trichoderma viride W2 is CGMCC No.4098. The trichoderma viride W2 can produce a new beta-glucosidase, the enzymatic activity of the new beta-glucosidase reaches 346.7U / mL, the optimal reaction pH value is 4.8, the optimal reaction temperature is 70 DEG C, and the new beta-glucosidase is suitable for pyrohydrolysis and has an obvious glucose feedback inhibition effect. The ethanol the concentration of which is 10% has a maximal effect on promoting the enzymatic activity and improves the enzymatic activity of the beta-glucosidase by 1.6 times, and the resistant ability of the ethanol reaches 30%, so that the cellobiose inhibition can be effectively eliminated, the yield of the ethanol is improved by nearly 3 times, and the terminal product inhibition is effectively eliminated. Thus, the beta-glucosidase can be used for simultaneous saccharification and fermentation of lignocellulose raw materials, has a rare promoting effect in China, effectively increases the yield of the cellulosic ethanol, lowers the production cost, and accelerates the industrialized progress of the cellulosic ethanol.

A beta-glucosidese, its encoding gene and use are disclosed. It consists of (a) or (b) protein, (a) protein is made of amino-acid sequence in sequence 3; (b) protein is derived by (a) and substituted and / or lost and / or added by one or several amino acid in sequence 3 and has beta-glucosidese. The process is carried out by: taking cellulose as material and synchronized mashing and common fermenting. It has better enzyme activity and can be used to produce alcohol.

The invention relates to a beta-glucosaccharase and its coding gene. The enzyme can keep high enzyme activity at high temperature and alkalinity condition. Its optimum reaction temperature is about 70centigrade degree; its pH value is about 8.0. It can catalyze and hydrolyze beta-glucose glycoside link to form glucose. It has important application value in foods, medicine, weaving, energy source, and so on.

The invention discloses an ultrafilter washing enzyme and manufacturing method to improve permeabile content through ultrafilter washing enzyme, which is composed of ultrafilter enzyme 1# and 3#, pectase, cellulose enzyme, xylanase, beta-glucanase, beta-glucosidase. The making method comprises the following steps: washing through alkalin; adding 1000-3000ppm / t ultrafilter enzyme 1# and 500-1000ppm / t amylase in the circulating system to circulate 30-60 min; washing through alkali again; adding 1000-3000ppm / t ultrafilter enzyme 3# in the system to circulate 30-60 min; separating retention material on the film surface; improving the effect of chemical abluent; improving membrane permeability.

The invention discloses a celluase producing bacterium. According to the invention, the classification name of the celluase producing bacterium is Penicillium piceum, the 18S rDNA (ribosomal deoxyribonucleic acid) gene sequence of a strain is shown in SEQ ID NO1, and the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preserving number of CGMCC5314 and the preserving date of October 9, 2011, Institute Microbiological Chinese Academy of Sciences, No.3 Beichen West Road 1 Chaoyang district, Beijing city. The celluase producing bacterium disclosed by the invention has good celluase and hemicellulase producing capabilities, and especially has good Beta-glucosidase production capacity. The celluase producing bacterium disclosed by the invention and the produced celluase and hemicellulase have wide application prospects in multiple industries such as biological fuel, medicine, daily chemical industry and food fermentation.

The invention relates to a method for preparing a high-color-value gardenia blue pigment. The invention aims to provide a method for preparing the high-color-value gardenia blue pigment through fully utilizing plenty of gardenoside in gardenia yellow waste liquor. The gardenia blue pigment prepared by adopting the method has the characteristics of high color value, high quality, high purity, environmental friendliness, and the like. The method for preparing the high-color-value gardenia blue pigment comprises the following steps of (1) gardenoside enzyme hydrolysis: carrying out enzyme hydrolysis on the gardenia yellow waste liquor with beta-glucosidase as the hydrolytic enzyme; (2) blue turning of the gardenoside hydrolysate: adding amino acid into the gardenoside hydrolysate obtained in the step (1) to react; (3) ultrafiltration separation of the gardenia blue pigment: carrying out ultrafiltration on the blued solution obtained in the step (2) by using an ultrafiltration membrane; and (4) extraction and purification of the gardenia blue pigment: drying trapped fluid obtained in the step (3) and carrying out extraction through mixing with methyl alcohol or ethyl alcohol to obtain the high-color-value gardenia blue pigment.

The invention relates to a high glucose resistance beta-glucosidase-CBD fusion enzyme, and expression gene and application thereof. The amino acid sequence is shown as SEQ ID NO. 1. Compared with the conventional beta-glucosidase enzyme, the fusion enzyme (BGL-CBD) provided by the present invention has the following characteristics: (1) higher cellulose binding ability, wherein the fusion enzyme (1 [mu]g / [mu]L) is incubated with 4 wt% of microcrystalline cellulose for 30min, and 98% of fusion enzyme is adsorbed by cellulose; (2) effectively degrading cellobiose, wherein the degradation rate of 10 wt% of the cellobiose by the fusion enzyme (0.15 [mu]g / [mu]L) within 8 h is 95%; and (3) resistance for high concentration glucose, wherein the glucose tolerance factor Ki is 1000 mmol / L.

The invention provides engineering bacteria based on beta-glucosidase in the technical field of bioengineering and an implementation method thereof. A beta-glucosidase gene is cloned from Streptomyces griseorubens (Streptomyces griseorubens) JSD-1, and then is connected to an expression vector to obtain a recombinant expression vector, and the recombinant expression vector is further introduced into escherichia coli and is induced to synthesize beta-glucosidase. The engineering bacteria provided by the invention overcomes the defects that the beta-glucosidase in the prior art is generally endoenzyme and the expression quantity is very low, and the like, the beta-glucosidase gene of the invention is expressed by genetically engineered bacteria to prepare beta-glucosidase, and a new method is provided for producing beta-glucosidase by industrial fermentation.

The invention discloses a method of efficiently converting algal polysaccharides to prepare bioethanol, comprising the steps of (1) pretreating materials by a physical method; (2) boiling in water bath; (3) enzymatically hydrolyzing with cellulase; (4) separating and filtering; (5) treating with glucoamylase and Alpha-amylase; (6) acid-hydrolyzing; (7) fermenting and distilling. The Alpha-amylase, glucoamylase, cellulase and Beta-glucosidase are added at a scientific ratio to pretreat the raw materials, and the conversion rate of algal polysaccharides can be increased; the converted algal polysaccharides are used to prepare fuel ethanol, and the yield of ethanol is increased; algal polysaccharides are utilized under high value, and marine biological energy sources are developed.

Disclosed is a method for improving tobacco extractive incense by multiple biological enzymes, which comprises charging pectolase, cellulose, hemicellulase and beta-glucosidase for enzymolysis, thus producing many kinds of scent generation constituents originally non-existent in the tobacco extract, as well as a plurality of micromolecular carbohydrates and beta-galacturonic acid.

The invention discloses a trichoderma reesei recombinant strain capable of highly producing cellulase and application thereof. Vlbltr which grows an incompatible repression protein gene is transformed into trichoderma reesei Rut-C30, so as to obtain the trichoderma reesei Vib1 (CCMCC No.13578). Compared with the strain Vib1 and the trichoderma reesei Rut-C30, the trichoderma reesei recombinant strain has the advantages that the excretion of extracellular protein and the production of cellulase are obviously improved; compared with the trichoderma reesei Rut-C30, the activity of cellulase in the Vib1 in cellulose and bran culture mediums is increased by about 200%, and reaches 3.3U / ml; the enzyme activities of cellobiohydrolase, endo-cellulase and beta-glucosidase are respectively improved; the excretion amount of the extracellular protein in the Vib1 strain is equal to 2.19 times of the excretion amount of trichoderma reesei Rut-30; proved by the results of hydrolysis reaction of pretreated corn straw, compared with the Rut-30 strain, the output of glucose in the Vib1 strain cellulase hydrolyte is increased by 20%.

The invention relates to the field of genetic engineering, in particular to medium-temperature beta-glucosidase BglA1, gene of the same and application of the same. The novel medium-temperature beta-glucosidase BglA1 contains amino acid sequence shown like SEQ ID NO.1. The invention further provides the gene for encoding the medium-temperature beta-glucosidase BglA1, recombination carrier containing the gene, recombination bacterial strain containing the gene and application of the medium-temperature beta-glucosidase BglA1, wherein nucleotide sequence of the gene is shown like SEQ ID NO.2. The optimum potential of hydrogen (pH) of the medium-temperature beta-glucosidase BglA1 is 6.0 and has higher enzymatic activity when the pH is from 5.0 to 7.0. Optimum temperature of the medium-temperature beta-glucosidase BglA1 is 50 DEG C, thereby being good in heat resistance. Thus, the medium-temperature beta-glucosidase BglA1 can be applied to industrial production with requirements for heat resistance.