406 results about "Beta-Glucosidases" patented technology

The invention relates to a natural tea essence prepared by using tea and a preparation method thereof. The tea is taken as a raw material; high-purity natural tea essences with different tea aroma characteristics are prepared through compound enzymolysis, releasing, steam distillation under reduced pressure, cold trap recycling, low temperature freezing concentration, centrifugal oil-water separation, and trace moisture adsorption by using anhydrous sodium sulfate; and the compound enzymolysis and the releasing are realized by adding pectinase, cellulase or pectinase and cellulose complex enzyme, and beta-glucosidase. In the preparation method, a pectinase and cellulose complex enzyme method is adopted for enzymolysis to facilitate cracking cell walls of the tea so as to facilitate liberation and separation of aroma components from the tea, and improve the yield of the essence; therefore, the method is a method for industrially producing the natural tea essence which is energy-saving, low in cost, environmentally friendly and high in efficiency.

The invention belongs to the biotechnology field and relates to a preparation method of genipin. The method comprises a preparation process of immobilized enzyme, a transformation process of gardenoside and a separation and purification process of genipin and is characterized by fermenting the strains for producing beta-glucosidase, collecting the fermentation liquor after reaching the enzyme producing peak, co-immobilizing enzyme and cells or enzyme and hyphae by combining embedding with crosslinking to prepare the immobilized enzyme, utilizing a packed-bed or stirred reactor to carry out catalyzed hydrolysis on the gardenoside and obtaining genipin after purification. The invention dispenses with separation and purification of the fermentation liquor, the immobilized enzyme obtained by preparation has high activity still keeping over 50% after operation for 960h and produces less pollution to the environment, genipin has high yield and the method has obvious advantages compared with other methods.

The invention provides a production method of a natural blue pigment, which comprises genipin prepared by reaction of beta-glucosidase catalyzing gargenoside, and natural gardenia blue pigment prepared by reaction between the genipin and amino acid in a proper condition. The production method is characterized in that metalloenzyme accelerator is added in the reaction of beta-glucosidase catalyzing gargenoside. Because the metalloenzyme accelerator is added in the reaction, the speed of enzymatic reaction can be accelerated and the reaction time is shortened, so that the production cost is saved, which is helpful for industrialized production. Meanwhile, metal ions added are all the trace elements that the human body needs, so the prepared pigment not only does not have the potential harm that the artificial pigment has, but oppositely has good health function, therefore the pigment can be applied in various fields like food industry.

Isolation plating medium of a first color for the identification of Listeria sp. and Listeria monocytogenes and Listeria ivanovii containing a first chromogenic substrate that responds to phosphatidylinositol-specific phospholipase C enzymes to release precipitate of a second color into the medium and a second chromogenic substrate that responds to beta-glucosidase enzymes to release precipitate of a third color into the medium, and said first, second and third colors contrasting with each other.

The invention discloses a saccharifying method of xylon cellulose through ultrasonic coordinated modified cellulose enzyme, which comprises the following steps: grinding and predisposoing xylon in the raw material through alkaline; reacting activated methoxy carbowax and cellulose enzyme in the citrate-sodium citrate buffer solution to obtain modified cellulose enzyme; blending modified cellulose enzyme, beta-glucosidase, amylase and pectase to obtain composite liquid; adding composite enzyme into predisposed raw material according to corresponding proportion; proceeding enzyme catalytic reaction through ultrasound; filtering; decompressing; evaporating; obtaining condensed sugar liquid.

The invention provides a preparation method and an application of a discarded tobacco leaf fermentation extract. Discarded tobacco leaves are fermented with beta-glucosidase, a tobacco biotransformation liquid containing various increased small-molecular aroma substances is obtained through enzymatic curing, then amino acid and reducing sugar in certain proportion are added for a high-temperature Maillard reaction under an alkaline condition, a natural tobacco Maillard reaction liquid is obtained and subjected to pumping filtration, a filtrate and filter residues are obtained, the filtrate is concentrated and mixed with propylene glycol and the like, further separation and purification are performed through molecular distillation, and light components are collected and taken as the discarded tobacco leaf fermentation extract. Reconstituted tobacco substrates with low irritation, low miscellaneous gas, low nicotine content and low harm are prepared from the filter residues with a paper-making process, the quality of the thin discarded tobacco leaf substrates is improved, the use effect of the discarded tobacco leaves on cigarette products is improved, the adding quantity of the extract is increased, the extract can be applied to an atomization filler material for novel carbon heating tobacco, and the quality of the atomization filler material is improved.

A plating media for identifying Yersinia pestis bacteria having nutrients for promoting growth of Yersinia pestis thereby producing beta-glucosidase, carbohydrate that is incapable of reacting with Yersinia pestis, a pH indicator dye, a chromogenic substrate that reacts to beta-glucosidase to form precipitate, and an agent to solidify the mixture, whereby a microorganism which ferments the carbohydrate but does not produce beta-glucosidase will produce colonies of the color determined by the pH indicator dye, Yersinia pestis and other microorganisms that do not ferment the carbohydrate but produce beta-glucosidase activate the substrate to color their colonies with the color of precipitate released by the substrate, and other bacteria which ferment the carbohydrate and produce beta-glucosidase produce colonies of the color that results from mixing the colors described above.

The invention discloses a penicillium deumbens engineered strain containing over-expressed beta-glucosidase, which contains over-expressed beta-glucosidase genes, is named penicillium deumbens Gi31-2 and is preserved in China Center for Type Culture Collection on May 7, 2010 with a preservation number of CCTCC M 2010111. The invention also discloses the application of the strain in production of the beta-glucosidase and degradation of cellulose. The highest activity of the beta-glucosidase of the engineered strain of the invention reaches 3.47 IU/ml and is 2.90 times as high as that of an original host strain, while highest filter paper activity is 3.32 IU/ml and is 1.23 times as high as that of the original host strain. Therefore, the strain is widely applied to the production of the beta-glucosidase.

The invention discloses a method for producing soybean isoflavone aglycone through microbe's enzyme method, and is culturing Aspergillus oryzae with soybean isoflavone powder as raw materials for fermentation to produce beta-glucosidase, and converting soybean isoflavone to soybean isoflavone aglycone. The method is characterized in comprising the following preparation steps: the said raw materials are 2-90% soybean isoflavone powder, the said strain is Aspergillus oryzae 3042, preparing seed culture liquid with the strain, preparing solid fermentation medium, fermenting to obtain fermenting product containing beta-glucosidase, adding soybean isoflavone powder for preparing enzyme converting solution, centrifuging for separation to obtain deposit, drying at low temperature to obtain soybean isoflavone aglycone. The invention avoids the restriction of the raw materials purity, converting rata can reach to over 90%. The invention has the advantages of short procedure, low cost, high bioavailability.

The invention relates to a novel beta glucosidase/xylosidase difunctional cellulose degradation enzyme RuGBGX2 as well as a coding gene and application thereof. The coding sequence of amino acid of the RuGBGX2 contains 18-755th sites of an SEQ ID NO 2 sequence. The RuGBGX2 is sourced from the rumen microorganism of yak from China, a novel coding gene of the beta glucosidase/xylosidase difunctional cellulose degradation enzyme RuGBGX2 is obtained by function screening and sequencing analysis on a rumen metagenome cosmid library and a subclone library. The beta glucosidase/xylosidase difunctional cellulose degradation enzyme provided by the invention can be widely applied to the degradation of cellulose and the fields such as cellulose biotransformation, chemical industry, spinning, foods, bioenergy, feed additives, medical industry and the like. By utilizing the difunctional enzyme RuGBGX2 to degrade wood fiber, the varieties of added enzymes can be reduced, and an enzymolysis process can be simplified.

The invention relates to a method for preparing rebaudioside through packed bed enzymatic hydrolysis of stevioside. The method comprises the following steps of: dissolving the stevioside or stevia rebaudiana extract in water or phosphate buffer solution; combining the parts containing rebaudioside of the solution through an immobilization beta-glucosidase packed column; and concentrating and recrystallizing to obtain the rebaudioside. The preparation of rebaudioside takes 5 days in the prior art, and the transformation activity of the beta-glucosidase repeatedly used for 4 times is only 57.72% of the original activity. Through the invention, the preparation of rebaudioside using the immobilization beta-glucosidase only takes 12 hours, and the transformation rate of stevioside after 5 times of use still exceeds 84%. Thus, the method provided by the invention is short in production period, high in production efficiency and easy to realize continuous production, and has very broad application prospects.

The invention relates to novel beta-glucosidase, and its coding gene and application. The invention further relates to an expression vector and host cells which contain the coding gene. The invention also relates to a method for using the beta-glucosidase to hydrolyze cellobiose generated in the process of cellulose degradation to glucose. The beta-glucosidase disclosed herein has the characteristics of high temperature resistance and good stability under neutral and alkaline conditions, and can be well applied in industrial production.

The invention discloses a trichoderma viride W2 capable of producing a thermophilic ethanol-resistant beta-glucosidase and an application thereof. The trichoderma viride W2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 23, 2010, and the preservation number of the trichoderma viride W2 is CGMCC No.4098. The trichoderma viride W2 can produce a new beta-glucosidase, the enzymatic activity of the new beta-glucosidase reaches 346.7U/mL, the optimal reaction pH value is 4.8, the optimal reaction temperature is 70 DEG C, and the new beta-glucosidase is suitable for pyrohydrolysis and has an obvious glucose feedback inhibition effect. The ethanol the concentration of which is 10% has a maximal effect on promoting the enzymatic activity and improves the enzymatic activity of the beta-glucosidase by 1.6 times, and the resistant ability of the ethanol reaches 30%, so that the cellobiose inhibition can be effectively eliminated, the yield of the ethanol is improved by nearly 3 times, and the terminal product inhibition is effectively eliminated. Thus, the beta-glucosidase can be used for simultaneous saccharification and fermentation of lignocellulose raw materials, has a rare promoting effect in China, effectively increases the yield of the cellulosic ethanol, lowers the production cost, and accelerates the industrialized progress of the cellulosic ethanol.

A beta-glucosidese, its encoding gene and use are disclosed. It consists of (a) or (b) protein, (a) protein is made of amino-acid sequence in sequence 3; (b) protein is derived by (a) and substituted and/or lost and/or added by one or several amino acid in sequence 3 and has beta-glucosidese. The process is carried out by: taking cellulose as material and synchronized mashing and common fermenting. It has better enzyme activity and can be used to produce alcohol.

The invention relates to a beta-glucosaccharase and its coding gene. The enzyme can keep high enzyme activity at high temperature and alkalinity condition. Its optimum reaction temperature is about 70centigrade degree; its pH value is about 8.0. It can catalyze and hydrolyze beta-glucose glycoside link to form glucose. It has important application value in foods, medicine, weaving, energy source, and so on.

The invention discloses an ultrafilter washing enzyme and manufacturing method to improve permeabile content through ultrafilter washing enzyme, which is composed of ultrafilter enzyme 1# and 3#, pectase, cellulose enzyme, xylanase, beta-glucanase, beta-glucosidase. The making method comprises the following steps: washing through alkalin; adding 1000-3000ppm/t ultrafilter enzyme 1# and 500-1000ppm/t amylase in the circulating system to circulate 30-60 min; washing through alkali again; adding 1000-3000ppm/t ultrafilter enzyme 3# in the system to circulate 30-60 min; separating retention material on the film surface; improving the effect of chemical abluent; improving membrane permeability.

The invention relates to a high glucose resistance beta-glucosidase-CBD fusion enzyme, and expression gene and application thereof. The amino acid sequence is shown as SEQ ID NO. 1. Compared with the conventional beta-glucosidase enzyme, the fusion enzyme (BGL-CBD) provided by the present invention has the following characteristics: (1) higher cellulose binding ability, wherein the fusion enzyme (1 [mu]g/[mu]L) is incubated with 4 wt% of microcrystalline cellulose for 30min, and 98% of fusion enzyme is adsorbed by cellulose; (2) effectively degrading cellobiose, wherein the degradation rate of 10 wt% of the cellobiose by the fusion enzyme (0.15 [mu]g/[mu]L) within 8 h is 95%; and (3) resistance for high concentration glucose, wherein the glucose tolerance factor Ki is 1000 mmol/L.

The invention provides engineering bacteria based on beta-glucosidase in the technical field of bioengineering and an implementation method thereof. A beta-glucosidase gene is cloned from Streptomyces griseorubens (Streptomyces griseorubens) JSD-1, and then is connected to an expression vector to obtain a recombinant expression vector, and the recombinant expression vector is further introduced into escherichia coli and is induced to synthesize beta-glucosidase. The engineering bacteria provided by the invention overcomes the defects that the beta-glucosidase in the prior art is generally endoenzyme and the expression quantity is very low, and the like, the beta-glucosidase gene of the invention is expressed by genetically engineered bacteria to prepare beta-glucosidase, and a new method is provided for producing beta-glucosidase by industrial fermentation.

The invention discloses a method of efficiently converting algal polysaccharides to prepare bioethanol, comprising the steps of (1) pretreating materials by a physical method; (2) boiling in water bath; (3) enzymatically hydrolyzing with cellulase; (4) separating and filtering; (5) treating with glucoamylase and Alpha-amylase; (6) acid-hydrolyzing; (7) fermenting and distilling. The Alpha-amylase, glucoamylase, cellulase and Beta-glucosidase are added at a scientific ratio to pretreat the raw materials, and the conversion rate of algal polysaccharides can be increased; the converted algal polysaccharides are used to prepare fuel ethanol, and the yield of ethanol is increased; algal polysaccharides are utilized under high value, and marine biological energy sources are developed.

Disclosed is a method for improving tobacco extractive incense by multiple biological enzymes, which comprises charging pectolase, cellulose, hemicellulase and beta-glucosidase for enzymolysis, thus producing many kinds of scent generation constituents originally non-existent in the tobacco extract, as well as a plurality of micromolecular carbohydrates and beta-galacturonic acid.

The invention discloses a trichoderma reesei recombinant strain capable of highly producing cellulase and application thereof. Vlbltr which grows an incompatible repression protein gene is transformed into trichoderma reesei Rut-C30, so as to obtain the trichoderma reesei Vib1 (CCMCC No.13578). Compared with the strain Vib1 and the trichoderma reesei Rut-C30, the trichoderma reesei recombinant strain has the advantages that the excretion of extracellular protein and the production of cellulase are obviously improved; compared with the trichoderma reesei Rut-C30, the activity of cellulase in the Vib1 in cellulose and bran culture mediums is increased by about 200%, and reaches 3.3U/ml; the enzyme activities of cellobiohydrolase, endo-cellulase and beta-glucosidase are respectively improved; the excretion amount of the extracellular protein in the Vib1 strain is equal to 2.19 times of the excretion amount of trichoderma reesei Rut-30; proved by the results of hydrolysis reaction of pretreated corn straw, compared with the Rut-30 strain, the output of glucose in the Vib1 strain cellulase hydrolyte is increased by 20%.