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Method of efficiently converting algal polysaccharides to prepare bioethanol

A seaweed polysaccharide and bioethanol technology, which is applied in the field of high-efficiency conversion of seaweed polysaccharides to prepare bioethanol, can solve the problems of not being able to actually increase the sugar concentration of fermentation raw liquid, high ethanol impurity content, and high condition requirements, so as to improve industrial economic benefits and improve conversion The effect of high efficiency and simple technical process

Inactive Publication Date: 2017-06-13
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods use seaweed raw materials to ferment bioethanol, the operation is complex, the condition requirements are high, the pollution to the environment is great, and the impurity content in the obtained ethanol is high and the concentration is low.
In addition, there are many methods that often use a relatively simple enzymatic hydrolysis, and add protease and pectinase to remove obstacles such as protein and pectin, which cannot actually increase the sugar concentration of the fermentation stock solution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Weigh 10g of dried Ulva powder into a 500ml flask, add 300ml of distilled water, cook for 5h in a water bath at 90℃; adjust the pH to 4.0 with HCl, add 30mg of cellulase and 15mg of β-glucosidase, and enzymatically digest at 40℃ for 8h. Use Ca(OH) 2 Adjust the pH to 5.0, add 600IU of liquefied enzyme, heat the hydrolysis in a water bath at 50℃ for 48h, adjust the pH to 4.0 with HCl, add 300IU of glucoamylase, and heat it in a water bath at 50℃ for 24h. After enzymatic hydrolysis, it was acid hydrolyzed with 2.0% sulfuric acid and treated at 100°C for 1 h. Centrifuge at 3000r for 10 min, and collect the supernatant. After sterilization at 100°C for 40 minutes, the activated Saccharomyces cerevisiae was inserted in the supernatant at a volume ratio of 15%, and then placed in a constant temperature water bath shaker set at 30°C for anaerobic fermentation. After 48 hours of fermentation , The ethanol is distilled out through the method of vacuum distillation, the yield of e...

Embodiment 2

[0067] Weigh 15g of dry Enteromorpha powder into a 500ml flask, add 450ml of distilled water, cook for 3h in a 95℃ water bath; adjust the pH to 4.5 with HCl, add 40mg of cellulase and 20mg of β-glucosidase, and enzymatically digest at 50℃ for 10h. Use Ca(OH) 2 Adjust the pH to 5.5, add 600IU of liquefaction enzyme, heat the enzymolysis in a water bath at 50℃ for 48h, adjust the pH to 4.5 with HCl, add 400IU of glucoamylase, and heat the enzymolysis in a water bath at 60℃ for 24h. After enzymatic hydrolysis, it was acid hydrolyzed with 2.5% sulfuric acid and treated at 105°C for 2h. Centrifuge at 3000r for 15min, and collect the supernatant. After sterilization at 105°C for 30 minutes, the activated Saccharomyces cerevisiae was inserted in the supernatant at a volume ratio of 20%, and then placed in a constant temperature water bath shaker set at 30°C for anaerobic fermentation. After 36 hours of fermentation The ethanol is distilled out through a vacuum distillation method, an...

Embodiment 3

[0069] Weigh 10g of dry Gelidium agaricula powder in a 500ml flask, add 350ml of distilled water, cook for 5h in a 95°C water bath; adjust the pH to 4.5 with HCl, add 40mg of cellulase and 20mg of β-glucosidase, and hydrolyze at 60°C for 10h. Use Ca(OH) 2 Adjust the pH to 6, add 800IU of liquefaction enzyme, heat the enzymolysis in a 60℃ water bath for 36h, adjust the pH to 5.5 with HCl, then add 600IU of glucoamylase, and heat the enzymolysis in a water bath at 70℃ for 24h. After enzymatic hydrolysis, it was acid hydrolyzed with 2.0% sulfuric acid and treated at 110°C for 2h. Centrifuge at 4000 r for 10 min, and collect the supernatant. After sterilization at 110°C for 40 minutes, the activated Saccharomyces cerevisiae was inserted into the supernatant at a volume ratio of 25%, and then placed in a constant temperature water bath shaker set at 35°C for anaerobic fermentation. After 24 hours of fermentation The ethanol is distilled out through the method of vacuum distillation...

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PUM

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Abstract

The invention discloses a method of efficiently converting algal polysaccharides to prepare bioethanol, comprising the steps of (1) pretreating materials by a physical method; (2) boiling in water bath; (3) enzymatically hydrolyzing with cellulase; (4) separating and filtering; (5) treating with glucoamylase and Alpha-amylase; (6) acid-hydrolyzing; (7) fermenting and distilling. The Alpha-amylase, glucoamylase, cellulase and Beta-glucosidase are added at a scientific ratio to pretreat the raw materials, and the conversion rate of algal polysaccharides can be increased; the converted algal polysaccharides are used to prepare fuel ethanol, and the yield of ethanol is increased; algal polysaccharides are utilized under high value, and marine biological energy sources are developed.

Description

Technical field [0001] The invention belongs to the field of seaweed utilization, and specifically relates to a method for efficiently transforming seaweed polysaccharides to prepare bioethanol. Background technique [0002] Energy shortage and environmental pollution are two major problems that the world must face today. Reducing the use of fossil fuels and developing clean renewable energy have become the only way to solve these two problems. Bioethanol has been widely studied in recent years because of its clean and renewable characteristics, especially its advantages in partially replacing fossil fuels. So far, many countries have achieved large-scale production of bioethanol, but most of these countries use corn, wheat, sugarcane and other food crops and sugar crops as raw materials to produce the first generation of bioethanol, which is a serious violation of The principle of energy development is to compete for food, not for land. Later, the second-generation bioethanol ...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12P19/20C12P19/14C12P19/04C12R1/865
CPCC12P7/06C12P19/04C12P19/14C12P19/20Y02E50/10
Inventor 李金花王吉萍王宗花
Owner QINGDAO UNIV
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