74results about How to "Lower conversion costs" patented technology

The invention discloses a method for preparing tobacco activated carbon based on low temperature plasma modification. The method is characterized by comprising the steps of: firstly drying and crushing waste and inferior tobacco into fine particles, and performing high temperature carbonization on the fine particles under nitrogen protection so as to obtain tobacco scorching samples; then putting the tobacco scorching samples into an inorganic acid solution for treatment, then cleaning the treated tobacco scorching samples until the cleaned tobacco scorching samples are neutral, putting the neutral scorching samples into an activator solution, macerating the neutral scorching samples in the activator solution, filtering the macerated scorching samples, and drying the filtered scorching samples; and finally, treating the scorching samples with low temperature plasma under activated gas atmosphere so as to obtain the tobacco activated carbon. According to the method disclosed by the invention, the waste and inferior tobacco in cigarette plants is directly prepared into the high-quality activated carbon, so that the cost of raw materials is low, and the purpose of locally transforming and utilizing the waste and inferior tobacco is realized. In addition, through the treatment of the low temperature plasma and under the joint action of fitting modification conditions, an activator and activated gas, the specific surface area of the tobacco activated carbon is enlarged, and the surface chemistry characteristics of the tobacco activated carbon are enriched.

The invention provides a CRISPR-Cas9 system-based genome editing vector and an application thereof, belongs to the field of plant genetic engineering and particularly relates to a CRISPR-Cas9 system-based wheat genome editing vector and an application thereof. A CRISPR-Cas9 transformation vector with higher editing efficiency in wheat is obtained through comparing the gene editing efficiency of the transformation vector of a CRISPR-Cas9 system when adopting different Cas9 promoters, different sgRNA promoters and different sgRNA sequence structures. Through the optimized vector, the gene editing efficiency of the wheat crop by using CRISPR-Cas9 is improved; the problems of low mutation rate of the CRISPR-Cas9 vector in the crops, such as the wheat after transformation and difficulty in obtaining of the CRISPR-Cas9 transgenic mutant strain are solved; the transformation time is shortened; the transformation cost is reduced; a better foundation is laid for popularization and application of a CRISPR-Cas9 gene editing system in the crop.

The invention relates to a soybean conversion method which comprises the following steps: cotyledon and the first euphylla of germinated soybean seeds are removed, and naked meristem is obtained; the naked meristem is inoculated to a pretreatment culture medium containing cytokinin for pretreatment; meristem blocks after the pretreatment are infected by agrobacteria. According to the soybean conversion method, the soybean meristem blocks after the pretreatment are used as explant materials for the first time; the explant materials are subjected to high-concentration cytokinin treatment before agrobacterium infection so that the meristem of soybeans can be in an active state and exogenous genes can be inserted easily; meanwhile, the meristem is multiplied and enlarged, so that the infection process is more convenient to operate; in the process of agrobacterium infection, ultrasonic treatment is added, so that the efficiency of insertion of the exogenous genes in a plant genome is greatly improved, and the conversion rate of the soybeans can reach 10%; meanwhile, the conversion period is short, the number of chimera is small, and conversion cost is low.

The invention relates to a method for improving the transformation efficiency of soybean. A method for selecting transformed plant cells comprises the following steps: transforming the plant cells by using a recombinant carrier of a gene containing a target gene and a gene for encoding sulfonylurea herbicide hydrolase; carrying out screening culture on the transformed plant cells by externally applying an ALS (Acetolactate Synthetase) inhibitor, and using the gene for encoding the sulfonylurea herbicide hydrolase as a selected marker; selecting the plant cells which are not killed and/or not inhibited. According to the method disclosed by the invention, a method of adding a selective agent into an enrichment culture medium and a differential culture medium by an external application mode (in particular to dropping) in the plant transforming process is put forward for the first time, and effective concentration screening range of the selective agent is optimized, and the proportion and the transformation efficiency of positive plants obtained by an offspring are remarkably improved; meanwhile, transgenic plants obtained by transformation using the sulfonylurea herbicide hydrolase as the selected marker have the advantages of high commercial value, good resistance and genetic stability.

The invention discloses a photocatalytic reactor for increasing the photocatalytic degradation rate of methane. The photocatalytic reactor comprises a reactor body with an upper opening, a reactor cover plate, an ultraviolet light source and a quartz coil capable of loading a photocatalyst for photocatalytic degradation of methane, wherein the reactor cover plate can be movably connected with the upper opening of the reactor body in a sealing way, the bottom of the reactor body with the upper opening is provided with an air inlet, and the reactor cover plate is provided with an air outlet; the quartz coil is vertically arranged inside the reactor body, one end of the quartz coil is connected with a water inlet arranged in the side wall of the reactor body, and the other end of the quartz coil is connected with a water outlet arranged in the side wall of the reactor body; the ultraviolet light source is arranged inside the reactor body, and the quartz coil is used for surrounding the ultraviolet light source. The photocatalytic reactor can realize the methane conversion ratio up to 71.5mg/(m<3>.min) under the condition of continuous light and has the characteristics of high catalytic efficiency and low conversion cost.

The invention discloses a dust collection device. The problems of poor collection effect and high electricity consumption amount of a dust collection device in the prior art are solved. A method adopted by the dust collection device for controlling an air suction motor to work comprises the following steps that 1, data is initialized; 2, when a waste sensor senses waste, a pulse number M, a pulsewidth value R and a pulse width change value delta R start to be calculated; 3, the pulse number M at the moment when preset time T is up is recorded, and a first pulse threshold value M1, a second pulse threshold value M2 and a pulse width change threshold value S are preset in a control circuit, wherein M1 is smaller than or equal to M2, and if the pulse number value M is smaller than or equal to M1 and the pulse width change value delta R is smaller than or equal to S, the first step is returned to; if M is larger than or equal to M1 and smaller than or equal to M2, the pulse width change value delta R is smaller than or equal to S, and the pulse width value R is decreased, the firs step is returned to; if the pulse number value M is smaller than or equal to M1, the pulse width change value delta R is smaller than or equal to S, and the pulse width value R is decreased, a PWM value output by the control circuit is decreased, and the rotation speed of the air suction motor is lowered; otherwise, the PWM value output by the control circuit is increased, and the rotation speed of the air suction motor is increased.

The invention discloses a preparation method of a deoxy analog of Echinocandin B. The method comprises the following steps of: (1) mixing a fermenting mixture of Echinocandin B with an organic solvent to obtain a solution 1; (2) mixing the solution 1 with an acid water solution to obtain a solution 2; and (3) standing the solution 2 at the temperature of 30-50 DEG C for 12-60h to obtain the deoxy analog of a compound shown as formula I, in the formula, R is linolenic acyl, R1=OH, R2=OH, and R3=OH; the organic solvent is selected from methanol, ethanol, ethyl acetate, acetone or chloroform; acid in the acid water solution is selected from hydrochloric acid, acetic acid or citric acid, wherein the concentration of the acid is 1-3mol/L; and pH of the solution 2 is 1-6.

The invention discloses a multi-core-shell structure nickel-based catalyst applied to the technical field of preparation of synthesis gas by reforming carbon dioxide, and a preparation method and a use method of the multi-core-shell structure nickel-based catalyst. According to the catalyst, bagasse subjected to hydro-thermal treatment is used as a carrier, nickel is used as an active component, magnesium is used as an auxiliary agent, the required catalyst is obtained after isopyknic impregnation, and the catalyst comprises, by the mass percentage, 15% of Ni, 5-15% of MgO and 70-80% of carbon. The prepared catalyst has high activity and stability, and can be kept active for at least 800 h in a carbon dioxide reforming stability experiment. The carrier of the catalyst uses low-cost bagasseas a raw material, hydrothermal carbon is prepared through a hydrothermal synthesis method, comprehensive utilization of the bagasse can be achieved, nickel and magnesium metals can be recycled through simple calcination of the used catalyst, cyclic utilization of the metals is achieved, and thus the purposes of reducing cost, saving resources and protecting the environment are achieved.

The invention discloses a universal expression vector for a large transgenic green alga and a transformation expression method thereof. Through studying the universality of a resistant screening marker and a resistant screening marker gene of large transgenic green algae, such as Enteromorpha prolifera, long Ulva, sea lettuce, and the like, corresponding resistant screening marker gene is cloned,the traditional carrier of the higher plant is adequately used on the basis or a homologous recombination technology is utilized to reconstruct the vector, and a universal carrier pSVB suitable for the large transgenic green alga is constructed. In the invention, on the basis of a cell regeneration system and a gametic parthenogenesis system, the efficiently transformed and stably expressed universal carrier is utilized to finally establish a large alga transformation and expression system so that the exogenous gene can be efficiently and stably expressed in the large algae, and the foundation for establishing an alga reactor and producing an important medicament and the alga energy sources in future is established.

A flow channel switching valve is provided that includes a stator and a rotor inside a housing, and rotates the rotor by a rotor drive portion while sliding the rotor on the stator, wherein the stator is fixed to a housing top by a stator fixing member. The stator fixing member is configured so as to have a portion detachably mounted on the housing top from a stator side, and a portion holding the stator.

The invention relates to transgenic maize, in particular to an agrobacterium-mediated mature maize embryo shoot apex transformation method. The method, which adopts maize shoot apex as a receptor, is not limited by genotype; and a set of strict culture medium system which is suitable for using the maize shoot apex as the transformation receptor is also developed, so that the efficient genetic transformation of the maize is achieved. The method disclosed by the invention shortens a maize transformation cycle, avoids genotype limitation by using the shoot apex as the receptor and also avoid such links as plant regeneration and the like in a transgenosis process. The method breaks through the limitation of explants transformation and improves the transformation efficiency of the maize. The method is simple and convenient to operate, simple and easy to operate, short in transformation cycle, high in transformation efficiency and reliable in result, and a transgenic maize plant can be rapidly obtained.

The invention relates to industrial by-product gypsum calcining equipment, which comprises a sting-out furnace, a kiln head, a roller, a feed hopper, an anhydrous gypsum discharge hole, a semi-hydrated gypsum discharge hole, a power transmission unit and a waste elevator. According to the invention, a temperature sensor is arranged at the discharge hole of a roller of an existing gypsum drying calcinator, and the discharge hole is also provided with a reversing valve and a discharge pipe. The temperature sensor detects the temperature of a material discharged out of the discharge hole of the roller. When the temperature in the roller is lower than the temperature of qualified gypsum, the temperature sensor controls the reversing valve for reversing, and unqualified gypsum products are conveyed into a waste gypsum storage tank. The waste elevator lifts and conveys unqualified gypsum into the feed hopper of the gypsum drying calcinatory, and then the unqualified gypsum is once again calcined and dried. Therefore, output of unqualified gypsum is reduced, output of qualified gypsum is raised, and raw materials and energy are saved.

The invention discloses a novel method for exchanging virtual currency for fishing commodities, comprising the following steps: a user wins gold coins in a game or gets diamonds through top-up; the user purchases a virtual fishing rod, bait, a fishing line and a fishing hook in the game with gold coins or diamonds, and participates in matches in the game to get fish bones; the method jumps to a real fishing online shopping mall through an exchange button in the game; stock commodities are displayed; the user exchanges the fish bones with a commodity displayed; the user gets a real commodity displayed in the online shopping mall from a local member center and a fishing shop through an order number, and exchange success is displayed; and the platform, the user, the member center and the fishing shop update data synchronously, and recheck and correct the number of fish bones and the number of real commodities. Through a mode of intelligent matching between user location and redeem point location, a nearby ground shop matching a user is found through screening, and the cost of exchange is reduced greatly. The value in the game tends to be equivalent to the commodity value in the real world, which contributes to exchange of virtual value for physical commodities.

The invention provides a data processing method and system. The method comprises the steps of obtaining a model exploration code corresponding to a preset format of an operator; and converting the model exploration code into a workflow corresponding to engineering according to a preset format of an operator. According to the embodiment of the invention, the code is directly converted into the workflow corresponding to the model project through the preset format of the operator without recoding, debugging and publishing the code in the model exploration process, so that conversion cost is reduced, and model exploration efficiency is improved.

The present invention relates to a corn transforming method, which comprises: obtaining primary callus by using a corn callus inducing method, obtaining a corn suspending cell line by using a corn suspending cell line obtaining method, and infecting the corn suspending cell aggregate in the corn suspending cell line through Agrobacterium. According to the present invention, the suspending cell line is rapidly obtained firstly from the primary callus induced by the corm immature embryo, such that the experimental period is shortened to 40-50 days, the operation is simple and convenient, the reproducibility is good, the genetic transformation of the corn is not affected by the season, and the annual stable transforming requirements are met; the corn suspending cell line is infected and transformed by using the Agrobacterium and then the seedling is generated, such that the transforming efficiency can achieve about 30%, the transgene positive rate can achieve about 90%, and the single copy rate is about 50%; and the corn explant (immature embryo) supply pressure is significantly reduced so as to substantially reduce the manpower and material resource consumption required by the explants culture and achieve the large-scale genetic transformation.

The invention discloses a die-free spinning machining clamp. The die-free spinning machining clamp comprises a clamp disc. The clamp disc is divided into a plurality of clamp petals; a plurality of clamping assemblies are uniformly distributed on clamping surfaces of the clamp petals around the center of the clamp disc; a rotary power transmission assembly is arranged in the center of the clamp disc and used for being connected with a spinning spindle and transmitting power to drive the clamp disc to rotate; a clamp petal traction assembly is further arranged in the center of the clamp disc; the clamp petal traction assembly comprises outer supporting guide rods penetrating through the rotary power transmission assembly; the outer supporting guide rods are connected with the clamp petals through outer supporting pull rods; and the plurality of clamp petals can be folded or separated along the radial direction along with the axial movement of the outer supporting guide rods. The die-free spinning machining clamp is high in universality, wide in size range and variety range of clamped machined parts, free of assistance of a die or a tail ejector, capable of effectively reducing the production cost and improving the spinning production efficiency and suitable for fixing various hollow thin-wall materials.

The invention relates to a method for transforming rice. The method comprises the following steps: obtaining callus by using a method for inducing rice callus or a method for greatly amplifying the rice callus, and infecting the callus by using agrobacterium. By adopting the method for transforming the rice, nonglutinous rice varieties which are not easy to have genetic transformation can obtain embryogenic callus, primary callus is firstly induced by using a solid culture medium and subsequently suspension culture is performed by using a liquid culture medium, so that a great deal of rice embryogenic callus with good quality and rapid growth can be amplified so as to be subjected to infection transformation by the agrobacterium; the transformation frequency can be greater than 10%, the pressure in nonglutinous rice explant supply is remarkably alleviated, and the time and the labor consumption in nonglutinous rice tissue cultivation are greatly reduced.