826results about How to "Improve editing efficiency" patented technology

A plurality of video materials specified out of video materials is managed as one group, information on each video material belonging to the group is displayed on a graphical user interface according to first operation, and according to second operation, a process to add a video material to the group or delete a video material specified out of the video materials belonging to the group is performed and the contents of a special effect set to a video material specified out of the video materials belonging to the group are managed in correspondence with only the video material.

The present invention provides a snap function that can move an object smoothly, without deteriorating the user operability. According to the present invention, there is provided an object editing system, including: an arrangement system for arranging an object and a snap target in a given display area; a moving system for moving the object in the display area; a snap system for causing the object to snap the snap target on a basis of a distance between the object and the snap target; and a restriction system for inhibiting the object from snapping the snap target on a basis of a direction in which the object is moving. According to the object editing system of the invention, the snap system causes the object to snap the snap target once the distance between the object and the snap target has become smaller than a predetermined threshold value.

A computer-implemented method for providing a candidate list of alternatives for a text selection containing text from multiple input sources, each of which can be stochastic (such as a speech recognition unit, handwriting recognition unit, or input method editor) or non-stochastic (such as a keyboard and mouse). A text component of the text selection may be the result of data processed through a series of stochastic input sources, such as speech input that is converted to text by a speech recognition unit before being used as input into an input method editor. To determine alternatives for the text selection, a stochastic input combiner parses the text selection into text components from different input sources. For each stochastic text component, the combiner retrieves a stochastic model containing alternatives for the text component. If the stochastic text component is the result of a series of stochastic input sources, the combiner derives a stochastic model that accurately reflects the probabilities of the results of the entire series. The combiner creates a list of alternatives for the text selection by combining the stochastic models retrieved. The combiner may revise the list of alternatives by applying natural language principles to the text selection as a whole. The list of alternatives for the text selection is then presented to the user. If the user chooses one of the alternatives, then the word processor replaces the text selection with the chosen candidate.

The invention relates to a method for preparing a CAR-T cell by CRISPR / Cas9. The method comprises integrating a CAR molecule into a first intron of a human 19th chromosome AAVS1 site by a CRISPR / Cas9 technology, and integrating antibody molecules of various tumor surface antigens into a human T cell genome AAVS1 site by CRISPR / Cas9. The method realizes accurate integration of the CAR molecule into a human T cell genome specific safe port site, does not influence human normal gene functions, and prevents a series of clinical risks such as viral vector safety hidden trouble and genetic toxicity and immunogenicity caused by insertion of an exogenous gene into a genome.

A computer program product for use with text transcribed from audio information, the computer program product residing on a computer-readable medium, includes computer-readable instructions for causing a computer to: enable defined keystroke combinations for use in editing the text transcribed from audio, the defined keystroke combinations corresponding to formatting functions identified as frequently-used functions during editing of a transcribed text; and respond to an activated keystroke combination by replacing a first format in the text document with a second format corresponding to the chosen keystroke combination.

A recording apparatus for recording video data to a rewritable optical disc is disclosed, that comprises an encoding means for encoding video data corresponding to a compression-encoding process, a converting means for converting the data structure of the encoded video data received from the encoding means into a file structure that allows a moving picture to be synchronously reproduced by computer software without need to use specially dedicated hardware, and a recording means for recording data having the file structure to an optical disc, wherein the file structure has a first data unit and a second data unit, the second data unit being a set of the first data units, and wherein a plurality of the second data units is matched with a successive record length of which data is written to the optical disc.

Provides subtitle generation methods and apparatus which recognizes voice in a presentation to generate subtitles thereof, and retrieval apparatus for retrieving character strings by use of the subtitles. An apparatus of the present invention includes: a extraction unit for extracting text from presentation documents; an analysis unit for morphologically analyzing text to decompose it into words; a generation unit for generating common keywords by assigning weights to words; a registration unit for adding common keywords to a voice recognition dictionary; a recognition unit for recognizing voice in a presentation; a record unit for recording the correspondence between page and time by detecting page switching events; a regeneration unit for regenerating common keywords by further referring to the correspondence between page and time; a control unit for controlling the display of subtitles, common keywords, text and master subtitles; and a note generation unit for generating speaker notes from subtitles.

The embodiment of the invention provides a video material processing method and device and a storage medium. The video material processing method comprises the steps that at least one to-be-edited video matched with a preset video editing template is determined in at least one video material according to the preset video editing template; and the at least one to-be-edited video is processed according to a preset video editing template to obtain a composite video. According to the preset video editing template, the at least one to-be-edited video matched with the preset video editing template is determined in the at least one video material, so that the style of the to-be-edited video is matched with the style of the preset video editing template, and the synthesized video obtained by processing is better in editing effect and higher in efficiency.

The invention discloses a method, a device and a system for online document edition. The method, the device and the system are applied to a process in which one same document is edited by at least two terminals by virtue of a server online; the method comprises the steps that the server receiving document edition operation information sent by each terminal, the server detects whether an edition operation conflict is present according to the document edition operation information sent by each terminal, and when the server finds out the edition operation conflict, the server sends the edition operation conflict information to the terminal where the document is edited so as to notice the terminal where the document is edited of the occurrence of the edition operation conflict and to notice the terminal having the edition operation conflict of processing the edition operation conflict. By use of the method, the device and the system, a plurality of terminals are enabled to edit a same paragraph simultaneously, and therefore, the edition efficiency is improved on the basis of avoiding the edition conflict.

The invention discloses a method for eliminating mecA plasmids based on CRISPR / Cas9 technology. The method comprises: selecting an MRSA strain to perform PCR amplification to a DNA sequence of a C terminal of mecA gene coding transpeptidase; carrying out gel extraction for mecA genes; connecting the mecA genes with a T-pMD19 (simple) carrier and preparing DH5[alpha] competent cells; electro-transforming the DH5[alpha] competent cells through the obtained T-pMD19-mecA plasmids; extracting the T-pMD19-mecA plasmids and performing sequencing and verification; performing double digestion treatment for the T-pMD19-mecA plasmids and performing gel extraction to the mecA genes; connecting pET-21a (+) plasmids with the mecA genes; designing and synthesizing oligos; constructing pCas9 :: mecA plasmids; electro-transforming the obtained pET-21a (+)-mecA plasmids into an escherichia coli expression strain BL21 (D3); and electro-transforming the pCas9 :: mecA plasmids and the pCas9 plasmids into BL21 (D3)+pET-21a(+)-mecA competent bacteria and BL21 (D3)+pET-21a(+) competent bacteria. The method is simple to operate and good in specificity, and can effectively block spread of mecA to eliminating the mecA strain.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 system and application thereof to construction of swine-derived recombinant cells with insulin receptor substrate gene defects. The invention provides an sgRNA (ribonucleic acid) combination which is composed of sgRNAIRS1-1, sgRNAIRS1-3, sgRNAIRS2-2 and sgRNAIRS2-3. The invention provides a plasmid combination. The plasmid combination is composed of four plasmids, and the four plasmids are respectively subjected to transcription to obtain sgRNAIRS1-1, sgRNAIRS1-3, sgRNAIRS2-2 and sgRNAIRS2-3. Theapplication of the sgRNA combination or the plasmid combination is as follows: preparation of recombinant cells; preparation of a diabetic cell model; preparation of a diabetic animal model. Accordingto the invention, sgRNAIRS1-1 is as shown in SEQ ID NO:8; sgRNAIRS1-3 is as shown in SEQ ID NO:10; sgRNAIRS2-2 is shown as SEQ ID NO:14; and sgRNAIRS2-3 is as shown in SEQ ID NO:15. A solid foundation is laid for the preparation of diabetic pig models, and important application value for the research and development of diabetic medicaments is achieved.

A method for editing an image on a display unit, an apparatus for executing the method and a computer program product for the method, wherein a predetermined CG object is designated in an image on the display unit, and in order to edit the image, a first command list for speech, motion, moving image reproduction or audio reproduction is displayed on the display unit, a command for editing the designated CG object is selected from the first command list and executed for the designated CG object. In the method of editing the image on the display screen and the apparatus for executing the method, the position information of the CG object located in the CG studio on the display unit and the information on the position and orientation of the camera for imaging the CG studio are read from a memory, it is determined whether the CG object selected by the pointing device is a CG character or a property, the information on the position to which the pointing icon has moved on the display unit by manipulating the pointing device is acquired, and the selected CG object is moved to the position to which the pointing icon has moved. Based on the information on the position to which the CG object has moved, the data on the character set-up window is updated in the case where the CG object is a CG character, and the data on the studio set-up window is updated in the case where the CG object is a property. The selected CG object can be dragged to the desired position by the pointing device.

The invention discloses a construction method and an application of the high-efficiency soybean CRISPR / Cas9 system. Staphylococus aureus Cas9 protein undergoes split expression; and in order to improve editing efficiency and broad spectrum, a soybean SaCas9 editing system is constructed. According to the construction method provided by the invention, a low-efficiency editing system is transformedinto the high-efficiency editing system, so that the application scope of editing soybean genome is widened, and meanwhile, a new thought is provided for solving a problem on crop genome editing efficiency in the future.

The invention aims at the technical defects of off-target effects of an existing CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated protein 9) gene editing technology to carry out site-directed mutation of an amino acid site on a site combined with a DNA (Deoxyribonucleic Acid) sequence of Cas9 protein to obtain the Cas9 protein with low off-target efficiency,so as to realize a gene editing unction with higher targeting performance and higher efficiency. Meanwhile, the invention further provides a method for carrying out HEK293T cell gene editing by adopting a gene editing method.

The invention discloses a video editing method and an electronic device. The method comprises the following steps: displaying a content label set and a style label set of the first video to be edited,wherein the content label set comprises at least one content label, each content label corresponds to at least one video clip in the first video, the style label set comprises at least one style label, each style label corresponds to at least one material combination, and each material combination comprises at least one edited material; receiving a first input of a user to the set of content tags; in response to the first input, intercepting a target video clip corresponding to the target content tag selected by the first input from the first video; receiving a second input of the user to thestyle label set; in response to the second input, obtaining a target material combination corresponding to the target style label selected by the second input; and synthesizing the target video clipand the editing material in the target material combination to generate a second video.

The invention provides specific synthetic chimeric xenonucleic acid guide RNA; s(XNA-gRNA) for enhancing crispr mediated genome editing efficiency. The invention also provides methods and compositions for inducing CRISPR / Cas-based gene editing / regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using single guide RNAs (sgRNAs) that have been chemically modified with xeno nucleic acids which enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a sgRNA that has been chemically modified with xeno nucleic acids to correct a mutation in a target gene associated with the genetic disease.

The invention provides a cotton promoter GbU6-7PS and application. The nucleotide sequence of the promoter is as shown in SEQ ID NO.1, and the nucleotide sequence of a complementary strand of the promoter is as shown in SEQ ID NO.2. The cotton promoter GbU6-7PS is obtained by conducting the first turn of PCR amplification so as to obtain a segment which covers full-length GbU6-7 promoter sequence, so that GbU6-7P is obtained; and then, conducting the second turn of PCR amplification, taking the GbU6-7P as a donor plasmid template and GbU6-5P::GUS as an acceptor plasmid template, and truncating and cloning the GbU6-7 promoter by virtue of Transfer PCR method, so that the cotton promoter GbU6-7PS is obtained. The cotton promoter GbU6-7PS disclosed by the invention is not only applicable to cotton, but also quite short in segment which is just 190bp long, conforming to the requirement of a CRISPR / Cas9 genome editing vector, the transcriptional level of sgRNA is significantly improved, and subsequently a genome editing efficiency may be increased.

The invention provides a novel enhancin CREnhancer 1.0 capable of increasing in vivo CRISPR / Cas9 gene editing efficiency obviously, provides a novel approach used for increasing gene editing efficiency in vivo, and provides a genome editing system with higher efficiency. When the enhancing and CRISPR / Cas9 are used together, CRISPR / Cas9 genome editing efficiency can be increased obviously.

The application relates to a set of artificial systm applied for site-specific alternation of rice genome basic group and a site-specific mutagenesis method. The artificial system comprises a I adjusting element and a II adjusting element; the I adjusting element comprises a nucleotide sequence capable of encoding an amino acid sequence I; the amino acid sequence I is selected from one of SEQ ID Nos. 1-6; the II adjusting element comprises a II-1 nucleotide sequence and a II-2 nucleotide sequence from 5' terminal to 3' terminal; the II-1 nucleotide sequence comprises a target nucleotide sequence; the II-2 nucleotide sequence comprises sgRNA nucleotide sequence originated from Streptococcus pyogenes; the II nucleotide sequence is transcribed and fused with the II-2 nucleotide sequence, and its product can guide the protein encoded by the I adjusting element to a target site to be mutated in the target biological genome, and induce and mutate C at the target site to be one of T, A and G, or G is mutated to be one of A, T and C.

The invention relates to an experimental method for constructing a Hutat2:Fc gene knock-in monocyte by using a CRISPR / Cas9 technology. The experimental method is characterized by comprising the following steps of (1) CRISPR / Cas9 targeting plasmid construction; (2) donor plasmid construction; (3) primary generation monocyte sorting; (4) monocyte transfection through electroporation; and (5) knock-in efficiency identification. The experimental method for constructing the Hutat2:Fc gene knock-in monocyte by using the CRISPR / Cas9 technology has the advantages that the method is simple and implemented easily, subsequent cell screening is not needed, the higher gene editing efficiency can be obtained, the feasibility and application prospect of the experiment are greatly increased, and the application range is wide.

The embodiment of the invention provides a video editing method and device specific to video materials of a plurality of lenses. The method comprises the following steps: creating an editing sequence in a first display window, wherein the editing sequence comprises editing tracks, and the quantity of the editing tracks is the same as the quantity of the lenses; adding the video materials of the lenses onto different editing tracks respectively; playing the video materials on the editing tracks at the same time in the first display window at a first preset playing speed; during playing of the video materials, extracting one or more video segments specified by a user; and splicing the extracted one or more video segments into a complete video according to a sequence of shooting times. Through the embodiment of the invention, the video editing efficiency can be increased greatly.

The invention discloses a video editing method and system, and a storage medium. The method comprises the steps of obtaining to-be-edited video data; editing the video data to be edited according to the index of the video playing time axis; wherein the index of the video playing time axis is generated according to at least one of a voice recognition result, a character recognition result and an image recognition result of the video data; wherein the index of the video playing time axis comprises at least one of a text index and an image index, and a one-to-one correspondence relationship is also established between the index of the video playing time axis and the video frame of the video data. According to the invention, video editing is assisted through the indexes of the video playing time axis generated by the voice recognition result, the character recognition result and the image recognition result, so that the video editing efficiency is improved. The corresponding video frames are directly positioned through the indexes of the video playing time axes in one-to-one correspondence with the video frames of the video data, so that editing is facilitated, the video editing efficiency is improved, and the operation experience of editing personnel is improved.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

The invention relates to rich text editing method and device based on an Android system. The method includes: 1, setting an operating menu and a test input box for the rich text editing device; 2, initializing the operating menu of the rich text editing device by Button monitoring events, and binding handling events for monitoring user operations; 3, responding to the handling events, receiving texts input by a user through the text input box, and sequentially bearing the texts to layers of a content container in a hierarchical manner; 4, etching the texts borne to the layers of the content container, and combining the edited layers to generate content satisfying a layout strategy; and 5, converting the edited data in the layers into character strings mapped from mapping relation of the layout strategy, and displaying the character strings and the text content in each layer by the text input box. The rich text editing method and device have the advantages that editing efficiency of rich texts is improved, the style of the edited rich texts is enriched, and personal needs of users are met.

The invention provides some new enhancins ESCS-higher, which can obviously improve the gene editing efficiency of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas9 in an epidermal stem cell. The invention provides a new way to improve the gene editing efficiency in the cell, and further provides a more efficient genome editing system. When the enhancin is used with the CRISPR / Cas9, the genome editing efficiency of the CRISPR / Cas9 in the epidermal stem cell can be obviously improved.

The invention provides a data processing method and device for a spreadsheet. The method comprises the following steps that: receiving a version comparison indication input by a user on the first version, which is edited at present, of the spreadsheet; according to the version comparison indication, canceling the editing of the user for the first version, and updating the first version into a second version, which is stored by a version management server at present, of the spreadsheet; determining the difference cell of the first version and the second version; and combining data in the difference cell, and submitting a combined version. Since the first version is updated into the second version before data is combined, the version number of the combined version is a second version number,the version number of the combined version is almost consistent with the version number of the version on the version management server so as to avoid the conflict of the spreadsheet, in addition, aplurality of users can carry out parallel execution, and the editing efficiency of the spreadsheet is improved.

The invention provides a multi-channel video and audio online-type playing and editing system which comprises a computer hardware system as an operation platform and a software system. The software system comprises a video and audio collecting module, a video and audio monitoring module, a video and audio marking and switching module, a video timeline module, an audio timeline module, a video and audio image-text composite file output module, a caption editing module, a caption listing module, a caption image-text timeline module and a video and audio image-text project file output module, wherein the video and audio collecting module is used for collecting signal sources, the video and audio monitoring module is used for monitoring the signal sources, the video and audio marking and the switching module is used for marking the collected signal sources, sending marked videos to the video timeline module to obtain editable video project files, sending marked audios to the audio timeline module to obtain editable audio project files and sending the videos and the audios to the video and audio image-text composite file output module to obtain composite finished video and audio files, the caption editing module, the caption listing module and the caption image-text timeline module are connected in sequence to form editable caption image-text project files, and the video and audio image-text project file output module is used for forming editable video and audio image-text project files. The multi-channel video and audio online-type playing and editing system can form finished files and editable project files and can achieve the functions of broadcasting director recall, simultaneous recording and editing, one-way recording and multi-way recording.