37results about How to "Improve editing efficiency" patented technology

The present application relates to the field of protein engineering, in particular to a fusion protein with an expanded PAM recognition range and application thereof. The fusion protein provided by the present application comprises: a) an amino acid sequence from a non-PI domain of xCas9; b) an amino acid sequence from a PI domain of a SpCas9 variant. The fusion protein has a more relaxed PAM restriction and significantly improved editing efficiency, and is a novel editing tool with an expanded targeting range.

This application discloses a view editing method, device, storage medium, and program product, relating to the field of smart home / intelligent home technology. The view editing method includes: determining the target snapping position of the target object in the floor plan based on the movement position of the target object and the floor plan; the movement position is generated by the user triggering the movement of the target object in the view editing interface; the target object includes: a device model or a furniture model; obtaining a target space view based on the floor plan, the target snapping position, and the target object; in the target space view, the target object is displayed at the target snapping position of the floor plan; this method adopts a drag-and-drop editing method, which is simple to operate, improves editing efficiency, and effectively ensures the geometric accuracy of the target object's spatial placement and the rationality of the view layout through automatic snapping.

This invention involves co-transfecting cells with an AAV vector plasmid carrying the target gene, a packaging plasmid providing Rep / Cap proteins, and a helper plasmid providing adenovirus assistance. The transfected cells are cultured under suitable conditions to produce viral particles containing recombinant AAV. Cells and culture supernatant are collected, cells are lysed to release the virus, and the viral particles are purified by centrifugation. A membrane-penetrating peptides or their derivatives are then modified onto the AAV capsid protein. Results show that the gene editing system combined with membrane-penetrating peptides or their derivatives enhances editing efficiency, effectively penetrates ocular surface tissues, and inhibits ocular neovascularization.

This application belongs to the field of biotechnology and relates to an efficient and specific editing system, method, and application for A-G base substitution. The fusion protein described in this application comprises, from the N-terminus to the C-terminus, a first SaCas9 nickase fragment, a chimeric deaminase fragment, and a second SaCas9 nickase fragment. The deaminase is selected from adenosine deaminase or a variant thereof, specifically ecTadA8e. The amino acid sequence of ecTadA8e is shown in SEQ ID NO. 20, or has more than 80% sequence identity with the amino acid sequence shown in SEQ ID NO. 20, and possesses the function or activity of ecTadA8e. The fusion protein provided in this application, combined with the corresponding guide RNA, can efficiently and specifically replace the base A with G at the target site, providing an effective tool for repairing pathogenic mutations, studying gene function, and improving cell function, and has promising application prospects.

The application discloses a method for creating non-transgenic mutant strains of Chinese cabbage. The method for creating non-transgenic mutant strains of Chinese cabbage provided by the application is realized by combining CRISPR / Cas9 technology and Chinese cabbage in situ transformation technology, and specifically comprises the following steps: introducing a CRIPSR / Cas9 gene editing vector carrying an sgRNA coding sequence of a target gene in a Chinese cabbage genome into a receptor Chinese cabbage by a vacuum infiltration in situ transformation method. Two PDS allele knockouts are obtained in 2032 seeds, and the non-transgenic editing mutant strains are obtained by the in situ transformation technology which is independent of tissue culture. The editing efficiency is greatly improved compared with the previously reported in situ transgenic efficiency, and the application provides a good research idea for transgenic safety and marker-free gene editing.

The invention discloses a model training method and device, an image processing method and device and electronic equipment, and belongs to the technical field of image processing. The method comprises the steps that a first training sample set is acquired, the first training sample set comprises multiple groups of training samples, and one group of training samples comprises a first face sample image, a first face reference image and sample editing information, editing the first face sample image based on the sample editing information to obtain the first face reference image; inputting the first training sample set into a first image processing model to obtain a first predicted face image output by the first image processing model, the first image processing model comprising a low-rank adaptive model; and according to the first predicted face image and the first face reference image, adjusting model parameters of the first image processing model to obtain a second image processing model.

The present application relates to the technical field of biotechnology, and particularly relates to a CRISPR / SuCas12a2-based gene editing system and application thereof. The present application constructs a high-efficiency counter-selection recombination editing tool by exploring the counter-selection potential of CRISPR-SuCas12a2 and combining it with lambda recombinase. The method can complete high-efficiency recombination editing of genes through a double-plasmid and single-plasmid system carrying SuCas12a2 and lambda recombinase, and the efficiency is greatly improved compared with the current recombination editing method based on lambda recombinase. Compared with the previous dependence on the specific cleavage of target double-stranded DNA by CRISPR-Cas protein to promote the effect of recombination editing, the efficiency is also obviously improved, and the method has the advantages of more transformants and low probability of immune escape. The plasmid construction, recombination editing and plasmid elimination operations can be completed within one week. The above functions supplement a powerful tool for gene recombination editing.

This invention discloses the application of chloroquine in improving gene editing efficiency. Specifically, it provides a novel use for chloroquine: enhancing the gene editing efficiency of gene editing reagents on recipient biological materials. The gene editing reagent is a gene editing tool plasmid. No prior art studies have combined chloroquine with gene editing efficiency. The inventors of this invention have discovered that treating recipient biological materials with chloroquine before transfecting them with gene editing reagents can significantly improve gene editing efficiency. This invention is simple to operate, widely applicable, and opens up a new avenue for improving editing efficiency by introducing exogenous small molecules rather than directly modifying gene editing tools.

The invention discloses application of hydroxychloroquine to improvement of gene editing efficiency. The invention provides novel application of hydroxychloroquine, namely application of hydroxychloroquine in improving gene editing efficiency of a gene editing reagent on a receptor biological material. In the prior art, a research of combining hydroxychloroquine and gene editing efficiency is not seen. The inventor of the invention finds that the gene editing efficiency can be remarkably improved by treating a receptor biological material with hydroxychloroquine and then transfecting a gene editing reagent. The method is easy and convenient to operate and wide in applicability, and a new way for improving the editing efficiency is developed by introducing exogenous small molecules instead of directly modifying a gene editing tool. The invention provides a basis for exploring an editing efficiency improvement strategy by adding exogenous molecules instead of modifying an editor mutant.

The invention relates to a frame selection interaction-based video element elimination method and device, equipment and a medium, and the method comprises the steps: responding to a frame selection operation of a user in a video preview region, generating a rectangular selection region covering a target elimination element, and obtaining the coordinate information of the rectangular selection region in an original video frame coordinate system; generating a binary mask image with the same resolution as the original video frame in the client based on the coordinate information; sending the original video frame and the binary mask image to a cloud server; performing content restoration on a corresponding region in the original video frame based on the binary mask image through an image elimination model to generate elimination result data; and returning the elimination result data to the client, and rendering and displaying the elimination result data in the video preview area to replace the original picture. According to the method, the target element area is accurately specified through the frame selection interaction mode, mask defects are reduced, the content repairing quality is improved, and meanwhile operation stability and editing efficiency are improved.

This invention relates to the field of gene editing technology, specifically to an Mb4Cas12a mutant protein, a CRISPR / Cas12a gene editing system using this mutant protein, and its applications. The complex formed by the Mb4Cas12a mutant protein and crRNA of this invention can precisely target DNA sequences and induce cleavage, causing double-strand breaks in the target sequence. It exhibits high specificity, reduces off-target rates in gene editing performed in cells or in vitro, and can distinguish single-base differences at target sites, thus possessing broad application prospects.

The invention provides a plasmid tool for simulating Kras random evolution and application thereof, and the plasmid tool comprises at least two guide RNA expression cassettes driven by a first promoter, which respectively target a Pten gene and a Trp53 gene; the pilot editing guide RNA expression box is driven by a second promoter, targets the 12th codon of the Kras gene, and comprises a repair template sequence for editing the codon into G12D; the fusion protein expression cassette is driven by a third promoter and comprises SpCas9 protein and MMLV reverse transcriptase; and at least one eukaryotic selection marker gene expression cassette. The invention also discloses a method for constructing a simulated Kras random evolution model by adopting the plasmid tool, and application of the plasmid tool in screening targeted drugs aiming at KRAS mutation tumors. The plasmid tool provided by the invention can realize cell multi-gene locus editing, construct a Kras locus random evolution model and simulate a Kras random evolution process in ovarian cancer.

This paper provides a table processing method, apparatus, electronic device, storage medium, and program product, applied in the field of human-computer interaction. The method includes: responding to receiving user input in a first table, outputting response information, whereby the user input includes table editing instructions composed of natural language, and the response information includes planning information generated based on the user input; and generating table content on a first page and / or a second page other than the first page of the first table based on the response information, whereby the table content includes at least one of the following: fields, row records, views, charts, workflows, and permission configurations. This paper solves the problems of cumbersome operation processes, low processing efficiency, and poor versatility. The corresponding beneficial effect is that it processes relevant tables based on received user input, thereby achieving intelligent table processing.

The application discloses a plant gene editing vector and a method and application of editing genes, and particularly provides a nucleotide sequence for encoding a CasWM protein after codon optimization, and a nucleic acid construct and a kit for gene editing. The optimized gene editing vector is introduced into plant cells, and high gene editing efficiency can be obtained, so that the plant traits can be improved, and new plant varieties can be cultivated.

The present disclosure relates to a thumbnail display method and device, electronic equipment, storage medium and program product. The method comprises: displaying a first track segment corresponding to a target video material on a video editing track, wherein a thumbnail of a first video frame in the target video material is presented in the first track segment; in response to a target operation, generating a thumbnail refresh request, wherein the target operation is used to trigger a display area of the target video material on the video editing track to change from the first track segment to a second track segment, and the thumbnail refresh request is used to obtain a thumbnail of a second video frame in the target video material; based on the thumbnail refresh request, generating the thumbnail of the second video frame by decoding the target video material; and displaying the thumbnail of the second video frame in the second track segment presented on the video editing track.

The application provides a bar screen integrated machine, which comprises a host computer and a plurality of displays. At least one integrated display card and a plurality of independent display cards are installed in the host computer, and the at least one integrated display card and each independent display card are connected with each display one by one. In this way, after the display connected with the at least one integrated display card is used as an editing end, the user can trigger an editing instruction at the editing end. After the editing end receives the editing instruction, the editing end can edit a plurality of bar screen contents to be displayed in one or more display ends in turn, and send the edited plurality of bar screen contents to the corresponding display end. After the display connected with each independent display card is used as a display end, the display end can be connected with an LED display screen through a sending card. In this way, the display end can send the received plurality of bar screen contents to the LED display screen for display in turn, or directly display the plurality of bar screen contents, thereby enriching the application scenarios.

The application discloses a video clip method and device based on ball hitting detection and a storage medium, relates to the technical field of videos, and comprises the following steps: in response to a clip trigger instruction, determining a core frequency response range according to a ball game scene recognition result of a to-be-processed video; performing frequency domain enhancement processing on original audio of the to-be-processed video to obtain audio data; extracting audio features of each evaluation dimension and determining whether the audio features all meet corresponding audio quality standards; if yes, determining a ball hitting moment in the to-be-processed video; aligning the ball hitting moment with the beat of background music and adjusting the playing speed of a ball hitting segment to obtain a target video. According to the application, the core frequency response range is determined according to the ball game scene, the audio is enhanced in the frequency domain, the ball hitting moment is recognized, and then the ball hitting moment is aligned with the beat of the background music and the speed is adjusted, so that the problem that the effect of ball hitting video clipping is poor is solved, and the to-be-processed video clipping efficiency, ball hitting recognition accuracy and finished product audio-visual adaptation effect are improved.

This application discloses a video editing method, apparatus, device, storage medium, and computer program product to address the problems of existing video editing solutions that rely heavily on manual operation, resulting in low editing efficiency, inability to handle the real-time editing needs of massive live videos, and highly subjective and inconsistent editing output. The method includes: sequentially extracting semantic features from each candidate video segment in an acquired set of candidate video segments to obtain a first semantic feature corresponding to each candidate video segment; determining a second semantic feature corresponding to the acquired editing requirements; selecting an editable video segment from the set of candidate video segments based on the feature similarity between the second semantic feature and the first semantic feature; and performing video editing based on the editable video segment.

This invention belongs to the fields of plant genetic engineering and biotechnology, and discloses a peanut endogenous strong promoter P. AhEF1α Its preparation methods and applications. Promoters include P AhEF1α‑1 P AhEF1α‑2 P AhEF1α‑3 and P AhEF1α‑4 This invention uses the Arabidopsis EF1α gene amino acid sequence as a reference, identifies candidate peanut EF1α genes through homology search, screens high-expression genes by expression level analysis, and clones the aforementioned promoter from the peanut genome of Yuhua 9326 using PCR technology. A recombinant vector containing this promoter is constructed and transformed into peanut embryogenic callus tissue. Functional verification shows that the promoter can efficiently drive the expression of downstream genes in peanut callus. This invention solves the technical bottleneck of existing peanut gene editing technologies that rely on exogenous promoters and have poor adaptability, fills the gap in the application of strong endogenous promoters in peanuts, and can be used to construct a precise gene editing system for peanuts, promoting stress-resistant, high-quality, and grade-equal peanut varieties. It has significant practical value and industrialization prospects.

This invention belongs to the field of gene editing technology, specifically relating to IscB / ωRNA gene editing mutants, editing systems, and applications. This invention truncates both the wild-type IscB protein and the wild-type ωRNA backbone to obtain IscB mutants and ωRNA backbone mutants. Compared to the wild-type IscB protein, the IscB mutants provided by this invention are smaller, have higher editing efficiency, and lower off-target efficiency; compared to the wild-type ωRNA backbone, the ωRNA backbone mutants provided by this invention are more compact while maintaining high editing capability. An IscB / ωRNA gene editing system (IscB) is established based on the ωRNA backbone mutants and IscB mutants of this invention. # / ωRNA # It has high editing efficiency and low off-target efficiency, and has been validated in different cell lines in vitro.

The invention discloses an automatic test method and device for an image editing task and electronic equipment, and relates to the technical field of computer vision, and the method comprises the steps: obtaining an input image and a target region mask, and enabling the target region mask to be located in the input image; generating a target editing instruction set based on the input image and the target area mask; editing the input image and the target area mask according to at least one editing instruction in the target editing instruction set to obtain an edited image; and performing dual quality evaluation on the editing area and the non-editing area to obtain a test result. Through dual quality evaluation, the problem that quality evaluation is incomplete due to the fact that a traditional evaluation method neglects regional differences is solved, the whole processes of preparation, execution and verification of the image editing test are connected into an automatic assembly line in series, manual intervention and waiting in all links are eliminated, and the test efficiency is remarkably improved.

The invention discloses a plant gene single base editing mutant genetic transformation efficient screening method, which realizes co-expression through P2A self-cleavage peptide fusion screening gene and base editing effector fusion gene, prolongs the window for playing the role, improves the base editing efficiency, and further obtains more target base editing mutant plants. Besides, a reconstructed vector skeleton contains a newly added screening gene and an original screening gene, and corresponding screening agents are sequentially used in a plant tissue culture process, so that the tissue culture burden caused by screening of a base editing effector protein high-expression transformation material is overcome.

The invention discloses an OpenCRISPR fusion protein for gene editing of cyprinid fishes and application of the OpenCRISPR fusion protein, and belongs to the technical field of fish gene editing. In order to solve the technical problem that a gene editor of cyprinid fishes is low in efficiency and the like, OpenCRISPR protein is designed by utilizing artificial intelligence, c-myc-NLS nuclear signal peptide is fused on the basis, the OpenCRISPR fusion protein is obtained, the editing efficiency of the OpenCRISPR fusion protein in the cyprinid fishes (such as zebra fishes) can be improved, and the OpenCRISPR fusion protein has an application prospect in gene editing of the cyprinid fishes.

The invention discloses a method for delaying senescence of leaves of non-heading Chinese cabbages by gene-edited persistent green genes SGR1 and SGR2, and belongs to the technical field of crop breeding. The method comprises the following steps: designing specific sgRNA according to coding region sequences of non-heading Chinese cabbage green-keeping genes SGR1 and SGR2, constructing a CRISPR / Cas9 gene editing vector, transforming a non-heading Chinese cabbage receptor material in a manner of infecting cotyledons with stems and hypocotyls by agrobacterium tumefaciens, regenerating adventitious buds, and screening to obtain a transformed plant containing a gene editing element, and screening out the SGR1 and / or SGR2 gene site-directed mutagenesis plants through molecular identification and phenotype observation. The staying green gene is accurately modified through a gene editing technology, the leaf senescence process of the non-heading Chinese cabbage is remarkably delayed, the leaf staying green property is improved, the fresh-keeping period is prolonged, excellent agronomic characters are kept, a new way is provided for genetic improvement and fresh-keeping technology development of the non-heading Chinese cabbage, and the method has important application value in the fields of vegetable production, fresh keeping and breeding.

The application discloses a vector for editing Nicotiana benthamiana genes based on CRISPR / Cas9 and a construction method and application thereof, and comprises BKG-g10 and / or BKG-g11 vectors constructed for a UbEF1B gene of the Nicotiana benthamiana and / or BKG-g12 and / or BKG-g13 vectors constructed for a CCR4 / NOT gene of the Nicotiana benthamiana. After the UbEF1B gene or the CCR4 / NOT gene is edited by the vectors, the growth state of the Nicotiana benthamiana is normal, and the phenotype does not obviously change compared with a wild type; the vectors do not affect the traits of the Nicotiana benthamiana, and are beneficial to the continuation of offspring. Compared with a traditional breeding method, the CRISPR / Cas9 system can more quickly and effectively obtain disease-resistant materials by editing endogenous genes of the Nicotiana benthamiana.

The invention belongs to the technical field of biological medicine, a gene editing system MMEJ-Exo is constructed through humanized application of exonuclease or key functional domains derived from microorganisms and eukaryotes, spCas9-mediated eukaryote cells are remarkably improved, especially long-fragment editing of primary neuronal cells is remarkably improved, and the gene editing efficiency is improved. And a new technology and a new method are provided for gene therapy and cell therapy transformation application.

The invention discloses a guide screen graph editing system and method based on a Web technology. The guide screen graph editing system comprises a front-end component framework, a front-end component framework and a rear-end component framework, wherein the front-end component framework comprises a main control component, a canvas component, a toolbar component and an attribute panel component; the graphic engine is integrated in the canvas component and is used for managing canvas rendering, a graphic object life cycle and an interaction event; the graphic editing module is used for drawing characters, geometric figures, local pictures and preset traffic symbols through a toolbar, generating a graphic object carrying a unique ID and storing the graphic object to the graphic object management array; the dynamic data binding module is used for dynamically binding the graphic elements with the real-time traffic data source; and the real-time rendering engine is used for responding to graphic attribute change or data binding operation and updating the canvas in real time through a graphic object event driving mechanism. According to the method, dynamic data direct binding is realized, graphic interaction and real-time editing functions are perfected, refined style control is provided, multi-resolution self-adaption is optimized, and the content editing efficiency and specialty of the induction screen are remarkably improved.

This invention belongs to the field of gene editing technology, specifically relating to an adeno-associated virus (AAV) delivery system based on IscB / ωRNA and its applications. This invention engineered IscB and its corresponding ωRNA to develop smaller, more efficient IscB protein and ωRNA backbones. Gene editing elements containing truncated IscB and ωRNA backbones are inserted between two inverted terminal repeat sequences to construct an IscB / ωRNA-based gene editing expression cassette. In the IscB / ωRNA-based gene editing expression cassette provided by this invention, a first promoter drives the expression of ωRNA, and a second promoter drives the expression of the IscB protein-encoding gene. The expressed ωRNA and IscB recognize and cleave the target gene, performing precise gene editing through self-cleaving homologous directed repair of nucleic acid donors, resulting in higher gene editing and delivery efficiency, and better safety.

This disclosure provides a method and system for visual editing and analysis of engineering deformation monitoring data, relating to the field of data processing technology. The method includes: loading an engineering cross-section base map and establishing a geographic coordinate system; responding to a measurement point placement command, setting up measurement points of the monitoring cross-section on the cross-section base map, establishing a mapping relationship between each set measurement point and the corresponding measurement point entity in the monitoring database; obtaining the deformation value under the target measurement based on the mapping relationship; calculating the geometric reference direction of the monitoring cross-section based on the spatial arrangement; calculating the deformation vector based on the geometric reference direction and the deformation value, the deformation vector including the deformation vector direction angle and the length of the deformation vector after scaling; determining the endpoint coordinates of the deformation vector based on the coordinates and the deformation vector, forming the original cross-section outline, the deformed cross-section outline, and the deformation area polygon, and color-filled rendering to generate a deformation distribution map displayed on a web page.

The invention relates to the technical field of gene editing, in particular to a CRISPR / Cas9 gene editing system and application thereof. The gene editing system is a complex formed by wild type or mutant type Hsp8Cas9 protein and sgRNA, and can accurately locate a targeted DNA sequence and cut the targeted DNA sequence, so that the target DNA sequence is subjected to double-strand breakage damage; the gene editing may be performed in a cell or in vitro. The wild type or mutant Hsp8Cas9 protein has a relatively small number of amino acids, and further, the editing specificity of the Hsp8Cas9 protein and the editing efficiency of the enHsp8Cas9 protein are high. The method has a wide application prospect in the field of gene editing.