294results about How to "Improve editing efficiency" patented technology

The invention relates to the technical field of artificial intelligence and the field of financial science and technology, and discloses a method for adjusting the playing speed of voice content in a video, and the method comprises the steps: obtaining a video file containing to-be-adjusted voice content, carrying out the voice recognition of an audio track of the video file, and converting the audio data of the voice content into text data; dividing the text data into a plurality of sentences or phrases; identifying keywords in each sentence or phrase, performing emotion content classification on the sentences or phrases containing the keywords, and determining the importance level of each sentence or phrase; according to the emotion content and the importance level, adjusting the playing speed of the corresponding voice content, and generating adjusted audio data; aligning the adjusted audio data with the video image to generate synchronous audio and video data; and a final video is generated. Through automatic voice recognition, sentiment analysis and playing speed adjustment, the steps of manual participation are reduced, and the video editing efficiency is greatly improved.

The invention discloses a multi-angle video automatic editing method based on content defect detection, and relates to the technical field of video processing. According to the method, multi-track video materials are accurately synchronized to the same time axis through cross-correlation calculation, and flaws in video content are analyzed frame by frame and classified and identified by applying fuzzy detection of a dynamic threshold and combining jitter detection and shielding detection of rotation and displacement estimation. And according to a detection result, an editing scheme is intelligently generated through a dynamic programming algorithm of state transition optimization, high-quality fragments are selected, defective parts are automatically removed, the continuity and multi-view diversity of video contents are reserved, and finally, a high-quality recorded video is generated through integration. The video editing efficiency is greatly improved, the manual intervention requirement is reduced, meanwhile, it is ensured that the quality of the output video meets the professional application requirement, and the method is suitable for multiple scenes such as conference recording, educational training and multi-angle video production and has high practical value and popularization prospects.

The invention discloses an optimized NlovFz2-omega RNA (Ribonucleic Acid) editing system, the optimal NlovFz2 system enNlovFz2 is obtained by modifying NlovFz2 protein and an omega RNA skeleton, and on the basis of remarkably improving the editing efficiency compared with a parent NlovFz2 system, the NlovFz2 mutant protein and T5 exonuclease are fused to obtain an enNlovFz2-T5 system with higher editing efficiency. The method has important significance and value for promoting wide application of a gene editing technology in the aspects of functional mechanism research and clinical treatment.

The invention discloses a fusion protein and application thereof. Fusion expression is carried out on R ring binding domains from different species sources and a single gene base editor, and it is found that compared with a base editor before fusion, the base editing efficiency of the obtained fusion protein is improved, and a base editing window is widened. According to the method, the R-ring binding structure domain is fused with the single-gene base editor, so that the base editing efficiency can be improved, and an editing window can be adjusted, so that the method can be suitable for more base editing scenes. The method is beneficial to popularization and application of a single-gene editing technology, and has wide application prospects in prevention and / or treatment of diseases and construction of animal models or plant varieties.

The invention relates to the technical field of three-dimensional scene editing, and discloses a three-dimensional scene editing method, device and equipment and a storage medium, and the method comprises the steps: converting a natural language editing instruction input by a target editing user into a step instruction and an operation instruction, extracting parameter index representation information, and based on an operation parameter standard model of the target editing user, carrying out the editing of a three-dimensional scene. And generating an operation parameter corresponding to the operation instruction under the step instruction, and finally constructing a three-dimensional scene based on the operation instruction and the operation parameter corresponding to each step instruction. According to the method, a three-dimensional editor is replaced by a three-dimensional scene editing framework based on natural language control by utilizing a large language model, a natural language editing instruction input by a user is converted into an operation parameter of a corresponding operation instruction under each step instruction, and an operation parameter standard model is introduced to record parameter index characterization habit characteristics of each user; the three-dimensional scene editing efficiency and the use flexibility are improved, and the three-dimensional scene editing operation threshold is reduced.

The embodiment of the invention provides a video processing method and device, a storage medium and a program product. In the method, a service platform can provide a service webpage, and a time axis manager and a video editor are embedded in the service webpage; a user can perform time axis editing on the original material based on the display area corresponding to the time axis manager, so that the original material is synchronously edited in the display area corresponding to the video editor. On one hand, the user can efficiently edit the video through the service webpage provided by the service platform, so that the performance requirement on the terminal equipment is reduced; and on the other hand, through cooperation between the time axis manager and the video editor, the user can perform time axis editing more efficiently based on the time axis manager so as to perform synchronous editing operation in the display area corresponding to the video editor more efficiently and view the editing state and the editing result of the edited original material more intuitively, and the user experience is improved. And the video editing efficiency is improved.

The invention discloses a control point parallel manual measurement method for image aerial triangulation. The method comprises the following steps: firstly, performing aerial triangulation on original image data with initial positioning parameters under the condition of no ground control, and optimizing geometric positioning parameters; secondly, dividing the complete measurement area into a plurality of subareas, predicting an image where a control point is located and an image point position of the image according to coordinates of the control point and image positioning parameters, cutting a local image block by taking the position as a center, and storing the local image block into a plurality of integrated image point data files according to the subareas; then, distributing the image point data file to a plurality of operators for parallelized control point measurement; and finally, the measured control point image coordinates are exported and recovered to an original measurement area project, and parallel measurement can be completed. According to the invention, the data portability is improved, the single-point measurement efficiency is improved, and parallel measurement is supported, so that the efficiency of large-data-volume control point measurement is improved to the maximum extent.

The embodiment of the invention provides a video editing method and device, electronic equipment and a storage medium. The method comprises the following steps: determining a target video material specified in a target editing task; generating a target text material corresponding to the target video material in the target editing task based on the related information of the target video material; wherein the editing operation indicated by the target editing task is at least used for presenting the target text material on an image of the target video material; and in response to a video generation instruction, performing editing processing on a material in the target editing task based on an editing operation indicated by the target editing task to obtain a target video. According to the scheme, the text material of the target video can be quickly edited and input under the assistance of text rewriting, and the video editing efficiency is improved.

The invention discloses a movie and television play intelligent shrinking method based on a large model, and the method comprises the steps: S1, carrying out the division processing of a movie and television play video and a plot introduction document, completing the segment division of the movie and television play video, and the plot paragraph division of the plot introduction document; s2, completing matching between the video clip and the plot paragraph by using a large language model; s3, generating a reduced script according to the plot introduction document by using a large language model; s4, the big language model completes the selection of the video clip in the step S1 based on the reduced script obtained in the step S3 and the matching relationship between the plot paragraph and the video clip obtained in the step S2; and S5, performing cross-modal retrieval in the range of the video clip selected in the step S4 by using the reduced story plot content to obtain a video picture. By intelligently generating the reduced script, the automatic editing process is realized, the manual operation is reduced, and the editing efficiency is improved.

The present invention discloses a recombinant glycosylase base editing system and its application. The recombinant glycosylase base editing system of the present invention is an APOBEC-nCas9-Ung1 or APOBEC(R33A)-nCas9-Rad51-Ung1 base editing system; the base editing system APOBEC-nCas9-Ung1 includes cytosine deaminase APOBEC, nuclease nCas9 and uracil DNA glycosylase Ung1; the base editing system APOBEC(R33A)-nCas9-Rad51-Ung1 includes cytosine deaminase APOBEC(R33A), nuclease nCas9, single-stranded DNA binding protein Rad51 and uracil DNA glycosylase Ung1. Experiments have shown that the recombinant glycosylase base editing system of the present invention can improve the editing efficiency of base C to base G in the genome sequence of an organism or a biological cell and the purity of the editing product.

The invention discloses a document collaborative editing method and device, a storage medium and a terminal, and the method comprises the steps: in response to an editing operation for a target document, determining first document data of the target document before the editing operation and second document data of the target document after the editing operation; splitting the second document data based on the first document data to obtain editing data corresponding to the editing operation; and sending the editing data to a collaboration server, so that the collaboration server issues the editing data to at least one collaboration terminal which is synchronizing the target document, and the collaboration terminal is used for updating local document data of the target document in the collaboration terminal according to the editing data. In the embodiment of the invention, the terminal splits the editing data corresponding to the editing operation according to the change of the overall document data of the target document before and after the editing operation, and then only sends the editing data to other collaborative terminals in real time, so that the collaborative terminals update the target document which is being displayed based on the editing data, and collaborative editing of the document is realized.

The invention relates to the technical field of biotechnology and plant genetic engineering, in particular to a rice two-way single-base edited co-transcription unit gene ABE-CBE system and application of the rice two-way single-base edited co-transcription unit gene ABE-CBE system. The rice bidirectional single base edited co-transcription unit gene ABE-CBE system provided by the invention comprises a co-transcription unit gene ABE-CBE, and the co-transcription unit gene ABE-CBE comprises an encoding gene ABE8e of adenine deaminase and an encoding gene Sdd7 of cytosine deaminase, and the coding gene ABE8e and the coding gene Sdd7 in the co-transcription gene ABE-CBE are constructed in the same expression vector. Through the designed ABE-CBE system, the two editors can be mediated at the same site to play a role at the same time, generation of unexpected base substitution can be further reduced, the editing efficiency of the base editors is improved, and wide application value is achieved.

The invention belongs to the technical field of gene engineering, and relates to application of an artemisia apiacea AaU6 promoter in CRISPR / Cas9 gene editing and a gene editing method, the promoter comprises AaU6-1, AaU6-4 and AaU6-8, the nucleotide sequence of the AaU6-1 is as shown in SEQ ID NO.1, the nucleotide sequence of the AaU6-4 is as shown in SEQ ID NO.2, and the nucleotide sequence of the AaU6-8 is as shown in SEQ ID NO.3. The invention also relates to an application of the artemisia apiacea AaU6 promoter in CRISPR / Cas9 gene editing. The promoter AaU6-1, the promoter AaU6-4 and the promoter AaU6-8 are utilized, sgRNA expression can be efficiently driven, the editing efficiency of a CRISPR / Cas9 gene editing system is improved, and great significance is achieved for breeding of sweet wormwood.

The invention provides a game scene editing method and device and electronic equipment, a graphical user interface for generating a content editor by a user is provided through terminal equipment, a game test scene is provided on the graphical user interface in response to a game trial playing instruction, and the game test scene is edited in response to a marking operation for the game test scene. Adding a target label to a target position of the game test scene, wherein the target label comprises editing guide information for the target position; and in response to the game editing instruction, providing a game editing scene in the graphical user interface, and displaying a target label at a target position in the game editing scene. According to the mode, by setting the label in the game test scene, the problems occurring in the game test scene can be marked, and the editing guide information can be set, so that a player can adjust the scene component in the game editing scene based on the label, the operation cost of game debugging and the problem memory cost of the player are reduced, and the user experience is improved. And the game editing and debugging efficiency is improved.

The invention relates to an editing interaction method and device and a computer readable storage medium, and relates to the technical field of terminals. The editing interaction method comprises the steps that a layer menu is displayed on an editing interface of the multimedia content, and the layer menu comprises at least one object entry corresponding to the multimedia content; in response to a touch operation of a user on the target object entry, displaying a submenu of the target object entry, the submenu including an operation function; and under the condition that the touch operation comprises a dragging operation and the target object entry is dragged to a trigger area of the operation function, responding to the user to stop the touch operation, and executing the operation function on an object corresponding to the target object entry. According to the method and the device, the operation convenience of the user in the editing process of the multimedia content is improved. And moreover, the probability of mistaken touch of the user is reduced through the simplified operation. Therefore, the editing efficiency can be improved on the whole.

The invention relates to an auxiliary editing method and system based on a rich text editor, and the method comprises the steps: selecting a text in response to the rich text editor, obtaining the selected text, adding a label, and displaying operation options, including "searching through a learning library" and "intelligently generating a picture"; in response to the operation option selected by the user, executing a corresponding auxiliary editing process; when the user selects'retrieval through the learning library ', calling a learning library service interface, querying by taking the tag text as a keyword, outputting a matched field, and displaying a'replacement' operation option; when the user selects'intelligent picture generation ', calling an AIGC question and answer interface to generate a picture, and displaying an'insertion' operation option; and in response to a replacement or insertion option selected by the user, matching the position information of the tag text through the regular expression, and replacing the tag text with the to-be-replaced field or inserting a picture behind the tag text. According to the method, the accuracy and flexibility of content editing are improved, and the method is suitable for various editing scenes.

The invention discloses application of a fusion protein KSa-SspCas9 and a mutant thereof in gene editing, and belongs to the field of genetic engineering. SspCas9 protein in Staphyloccus sp.17KM0847 bacteria is excavated through analysis of bioinformatics, the protein has PAM recognition capability of NNG, a PI structural domain of the SspCas9 protein is fused with a non-PI structural domain of the SaCas9 protein to obtain the fusion protein KSa-SspCas9, and the fusion protein KSa-SspCas9 is used for gene editing. The fusion protein has higher base editing efficiency. Meanwhile, the specific site of the fusion protein is mutated, and the PAM recognition range is greatly widened through PAM sequences of NRN and NYN which can be recognized by mutants. Through discovery of the Cas9 protein and the gene editing system, the variety of gene editing tools is expanded, and the Cas9 protein and the gene editing system have wide application prospects in the aspects of disease treatment, animal model preparation, plant breeding and the like.

The invention belongs to the field of gene engineering. Specifically, the invention relates to a system, a tool and a method for modifying or editing target nucleotide bases, and more specifically, the invention relates to an optimized QBE base editing system and application thereof. The base editing system disclosed by the invention has the advantages of high efficiency of a gene editing tool, high product purity, low Indel frequency, low off-target frequency and abundant gene editing windows, and has important value for expanding application scenes of a base editing technology in the fields of life science basic research, agriculture, biological medicine and the like.

The invention provides a method for efficiently preparing FGF5 homozygous knockout cashmere goats by combining a base editor with a somatic cell cloning technology. And an sgRNA site is designed. An sgRNA expression vector is constructed, the sgRNA expression vector and pCMV-AncBE4max are respectively transferred into fibroblasts of a down producing goat fetus, and the editing efficiency is analyzed. And optimizing an electrotransfection condition, and simultaneously transfecting the screened sgRNA expression vector and pCMV-AncBE4max into a down producing goat fetal fibroblast to obtain the FGF5 homozygous knockout cell strain. And preparing reconstructed embryos and FGF5 homozygous knockout cashmere goats. The average editing efficiency of the sgRNA site disclosed by the invention can reach 64.3 + / -8.1%. The average probability of FGF5 homozygous knockout cells is 69.0%. Wherein the homozygous probability is 58.6% on average. The lambing rate is 7%. The invention provides a theoretical basis and a research basis for cultivating high-yield and high-quality new varieties.

The embodiment of the invention provides a video generation method and device, a storage medium and a program product. In the method, a video editor can be used for analyzing multimedia elements of file pages with data formats supported by the video editor and original attribute information of the multimedia elements, and a time axis corresponding to each multimedia element can be edited on a canvas page corresponding to each file page. And adjusting the display time of each multimedia element to obtain target attribute information of each original material, and generating a video corresponding to any file page based on each original material corresponding to any file page and the target attribute information thereof. On one hand, the user makes the video through the video editor, and the efficiency of converting the PPT file into the video is improved; and on the other hand, the time editing operation is synchronized in the display area corresponding to the video editor more efficiently through the time axis, and the editing state of the edited original material is viewed more intuitively, so that the video editing efficiency is improved.

The invention discloses a picture and text joint editing and correcting method and system for publications, and belongs to the technical field of publication. The method comprises the steps that all pictures and picture names in the publications are read; matching the picture with the picture name; and taking the picture and the picture name which are matched with each other as input, taking related information of the publication as a prompt condition, and performing joint editing and correction on the picture and the picture name which are matched with each other through the multi-modal large model to obtain a joint editing and correction result. Compared with an existing method, the method has the advantages that the editing and correcting efficiency of the publication can be obviously improved, pictures and picture name contents in the publication can be edited and corrected in a combined mode, missing pictures and picture names in the publication can be supplemented, and the supplemented pictures and picture name contents can be edited and corrected in a combined mode. According to the method, the labor cost of publication editing work can be reduced, and harmful information can be automatically screened and prevented from flowing into the publishing market.

The invention provides a component editing method and device in a game and electronic equipment, and the method comprises the steps: responding to an editing operation for a target scene component in a game editing scene, determining a target editing mode, displaying an operation control corresponding to the target editing mode in a graphical user interface, and enabling the operation control to comprise triggering areas corresponding to a plurality of preset axial directions; and in response to a sliding operation aiming at a triggering area corresponding to a target preset axial direction in the operation control, controlling the target scene component to be adjusted along the target preset axial direction based on the target editing mode and the sliding operation so as to adjust a display form or a display position of the target scene component in the game editing scene. According to the mode, when component editing is carried out in the game editing scene, the operation control can be displayed in the interface, so that a player can edit the display form and the display position of the component by operating the rocker, the situation that the hand moves into the game editing scene to edit the component is avoided, mistaken touch in the component editing process is reduced, and the component editing efficiency is improved.

The invention relates to the field of biotechnology and biological breeding, and particularly relates to a method for constructing a single-chain guide RNA (sgRNA) vector for delivering CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) by utilizing a cucumber mosaic virus (CMV), carrying out site-specific editing on a target gene in a Cas9 transgenic plant, causing site-specific mutagenesis of plant genome DNA (Deoxyribose Nucleic Acid) by the vector to be inherited to a progeny, and not carrying the CMV in the progeny. The invention discloses a construction method of a vector pCB301R3-sgPDS and an application of the vector pCB301R3-sgPDS. The pCB301R3-sgPDS is a genetic gene editing vector based on mediation of a cucumber mosaic virus. According to the invention, sgRNA is delivered through CMV for the first time, so that fixed-point editing and seed transmission of plant target genes are efficiently realized in crops.

The invention relates to the technical field of urinary tract infection detection, and discloses a phage-based rapid and accurate urinary tract infection detection method, which comprises the following steps: S1, constructing a CRISPR / Cas9 system; s2, constructing a homologous recombinant plasmid; s3, constructing three plasmids BL21; s4, constructing, screening, verifying and reporting a phage T4:: Nluc; according to the detection method, a CRISPR / Cas9 system is combined with a homologous recombination technology, so that the efficiency of bacteriophage gene editing is remarkably improved, based on the report bacteriophage T4:: Nluc, rapid, sensitive, specific, accurate, simple and convenient detection on clinical urinary tract infection escherichia coli is realized, and the method can be used for detecting an escherichia coli urinary tract infection sample which is not pretreated, and has a wide application prospect. The kit shows excellent detection timeliness and specificity, is a powerful tool for identifying clinical urinary tract infection escherichia coli, and provides a new technical path for timely and accurate diagnosis and effective treatment of clinical urinary tract infection.

The invention provides a plant cytosine single-base editor as well as a construction method and application thereof, and relates to the technical field of plant genetic engineering. The single-base editor provided by the invention is named as hyPopCBE, and has a structural formula shown in the description, the editor comprises hA3A / Y130F cytosine deaminase, an optimized human-derived Rab51 single-stranded DNA binding protein structural domain, a fusion protein of nCas9 nuclease and a uracil glycosylase inhibitor, a fusion protein expression unit of phage MS2 coat protein and the uracil glycosylase inhibitor, and a guide RNA transcription expression unit integrated with an MS2 binding sequence. Compared with an existing plant cytosine single-base editor, the single-base editor constructed by the method can remarkably improve the editing efficiency of plant alleles, and the stability is higher; when the herbicide-resistant poplar gene is applied to poplar, a new herbicide-resistant poplar germplasm can be successfully created, and a technical support is provided for research and molecular breeding of a poplar functional genome.

The invention discloses a method for improving the editing efficiency of an apple CRISPR / Cas9 system by using an improved leaf disc conversion method, and belongs to the technical field of biology. The invention provides a culture medium composition according to the requirement of woody plant tissue culture nutrients, the requirement of apple leaf regeneration can be met, calluses and regenerated buds with high differentiation capacity are induced, and the problem of low genetic transformation efficiency of apples is solved. An improved leaf disc conversion method based on the culture medium package can significantly improve the editing efficiency of an apple CRISPR / Cas9 system, can efficiently deliver a CRISPR / Cas9 knockout carrier, obtains homozygote and heterozygote plants of different mutation types including deletion, replacement, insertion and the like, and has the editing efficiency as high as 92.3%. The method provided by the invention provides a new thought for analyzing genetic mechanisms of apples, improving agronomic characters and performing germplasm innovation by utilizing a gene editing technology.

The invention provides sgRNA and CRISPR / Cas9 vectors, a construction method of the sgRNA and CRISPR / Cas9 vectors and application of the sgRNA and CRISPR / Cas9 vectors in poplar gene editing, and relates to the technical field of plant genetic engineering. The specific sgRNA is obtained by optimizing the length and the repetition number of the sgRNA, the CRISPR / Cas9 system is constructed on the basis of the sgRNA, and the CRISPR / Cas9 system can remarkably improve the editing efficiency of the poplar endogenous genes PagRbcS and PagHEMA, and particularly improve the editing efficiency of the monoalleles and dialleles of the poplar endogenous genes.

The invention relates to the technical field of gene engineering and cell engineering, in particular to an sgRNA combination for precisely editing a dairy cow BLG gene, a primer and application. The sgRNA combination comprises: sgRNA1, the sequence of which is as shown in SEQ ID NO. 1; the sequence of the sgRNA2 is as shown in SEQ ID NO. 2; the sequence of the sgRNA3 is as shown in SEQ ID NO. 3; and the sequence of the sgRNA4 is as shown in SEQ ID NO. 4. The BLG gene edited mammary epithelial cells and the BLG gene single base edited dairy cow can be successfully obtained, and the method has a wide application prospect in the aspect of dairy cow breeding.

The invention is suitable for the technical field of block chains, and provides a byte code editing method and device, electronic equipment and a program product. The byte code editing method comprises the following steps: acquiring a first byte code to be edited; editing the first byte code to obtain a second byte code; according to the first byte code and the second byte code, determining whether the length of the byte code is changed or not; and if it is determined that the length of the byte code changes, adjusting the second byte code to obtain a target byte code, and outputting the target byte code. Through the editing method provided by the invention, after the byte code is edited, the edited byte code can be automatically checked and adjusted through the electronic equipment, so that the editing efficiency of the byte code editing method is improved.

The present application relates to the field of protein engineering, in particular to a fusion protein with an expanded PAM recognition range and application thereof. The fusion protein provided by the present application comprises: a) an amino acid sequence from a non-PI domain of xCas9; b) an amino acid sequence from a PI domain of a SpCas9 variant. The fusion protein has a more relaxed PAM restriction and significantly improved editing efficiency, and is a novel editing tool with an expanded targeting range.