83results about How to "Improve editing efficiency" patented technology

The invention discloses an optimized NlovFz2-omega RNA (Ribonucleic Acid) editing system, the optimal NlovFz2 system enNlovFz2 is obtained by modifying NlovFz2 protein and an omega RNA skeleton, and on the basis of remarkably improving the editing efficiency compared with a parent NlovFz2 system, the NlovFz2 mutant protein and T5 exonuclease are fused to obtain an enNlovFz2-T5 system with higher editing efficiency. The method has important significance and value for promoting wide application of a gene editing technology in the aspects of functional mechanism research and clinical treatment.

The embodiment of the invention provides a video editing method and device, electronic equipment and a storage medium. The method comprises the following steps: determining a target video material specified in a target editing task; generating a target text material corresponding to the target video material in the target editing task based on the related information of the target video material; wherein the editing operation indicated by the target editing task is at least used for presenting the target text material on an image of the target video material; and in response to a video generation instruction, performing editing processing on a material in the target editing task based on an editing operation indicated by the target editing task to obtain a target video. According to the scheme, the text material of the target video can be quickly edited and input under the assistance of text rewriting, and the video editing efficiency is improved.

The present invention discloses a recombinant glycosylase base editing system and its application. The recombinant glycosylase base editing system of the present invention is an APOBEC-nCas9-Ung1 or APOBEC(R33A)-nCas9-Rad51-Ung1 base editing system; the base editing system APOBEC-nCas9-Ung1 includes cytosine deaminase APOBEC, nuclease nCas9 and uracil DNA glycosylase Ung1; the base editing system APOBEC(R33A)-nCas9-Rad51-Ung1 includes cytosine deaminase APOBEC(R33A), nuclease nCas9, single-stranded DNA binding protein Rad51 and uracil DNA glycosylase Ung1. Experiments have shown that the recombinant glycosylase base editing system of the present invention can improve the editing efficiency of base C to base G in the genome sequence of an organism or a biological cell and the purity of the editing product.

The invention relates to an editing interaction method and device and a computer readable storage medium, and relates to the technical field of terminals. The editing interaction method comprises the steps that a layer menu is displayed on an editing interface of the multimedia content, and the layer menu comprises at least one object entry corresponding to the multimedia content; in response to a touch operation of a user on the target object entry, displaying a submenu of the target object entry, the submenu including an operation function; and under the condition that the touch operation comprises a dragging operation and the target object entry is dragged to a trigger area of the operation function, responding to the user to stop the touch operation, and executing the operation function on an object corresponding to the target object entry. According to the method and the device, the operation convenience of the user in the editing process of the multimedia content is improved. And moreover, the probability of mistaken touch of the user is reduced through the simplified operation. Therefore, the editing efficiency can be improved on the whole.

The invention relates to an auxiliary editing method and system based on a rich text editor, and the method comprises the steps: selecting a text in response to the rich text editor, obtaining the selected text, adding a label, and displaying operation options, including "searching through a learning library" and "intelligently generating a picture"; in response to the operation option selected by the user, executing a corresponding auxiliary editing process; when the user selects'retrieval through the learning library ', calling a learning library service interface, querying by taking the tag text as a keyword, outputting a matched field, and displaying a'replacement' operation option; when the user selects'intelligent picture generation ', calling an AIGC question and answer interface to generate a picture, and displaying an'insertion' operation option; and in response to a replacement or insertion option selected by the user, matching the position information of the tag text through the regular expression, and replacing the tag text with the to-be-replaced field or inserting a picture behind the tag text. According to the method, the accuracy and flexibility of content editing are improved, and the method is suitable for various editing scenes.

The invention belongs to the field of gene engineering. Specifically, the invention relates to a system, a tool and a method for modifying or editing target nucleotide bases, and more specifically, the invention relates to an optimized QBE base editing system and application thereof. The base editing system disclosed by the invention has the advantages of high efficiency of a gene editing tool, high product purity, low Indel frequency, low off-target frequency and abundant gene editing windows, and has important value for expanding application scenes of a base editing technology in the fields of life science basic research, agriculture, biological medicine and the like.

The invention provides a method for efficiently preparing FGF5 homozygous knockout cashmere goats by combining a base editor with a somatic cell cloning technology. And an sgRNA site is designed. An sgRNA expression vector is constructed, the sgRNA expression vector and pCMV-AncBE4max are respectively transferred into fibroblasts of a down producing goat fetus, and the editing efficiency is analyzed. And optimizing an electrotransfection condition, and simultaneously transfecting the screened sgRNA expression vector and pCMV-AncBE4max into a down producing goat fetal fibroblast to obtain the FGF5 homozygous knockout cell strain. And preparing reconstructed embryos and FGF5 homozygous knockout cashmere goats. The average editing efficiency of the sgRNA site disclosed by the invention can reach 64.3 + / -8.1%. The average probability of FGF5 homozygous knockout cells is 69.0%. Wherein the homozygous probability is 58.6% on average. The lambing rate is 7%. The invention provides a theoretical basis and a research basis for cultivating high-yield and high-quality new varieties.

The invention provides a plant cytosine single-base editor as well as a construction method and application thereof, and relates to the technical field of plant genetic engineering. The single-base editor provided by the invention is named as hyPopCBE, and has a structural formula shown in the description, the editor comprises hA3A / Y130F cytosine deaminase, an optimized human-derived Rab51 single-stranded DNA binding protein structural domain, a fusion protein of nCas9 nuclease and a uracil glycosylase inhibitor, a fusion protein expression unit of phage MS2 coat protein and the uracil glycosylase inhibitor, and a guide RNA transcription expression unit integrated with an MS2 binding sequence. Compared with an existing plant cytosine single-base editor, the single-base editor constructed by the method can remarkably improve the editing efficiency of plant alleles, and the stability is higher; when the herbicide-resistant poplar gene is applied to poplar, a new herbicide-resistant poplar germplasm can be successfully created, and a technical support is provided for research and molecular breeding of a poplar functional genome.

The invention discloses a method for improving the editing efficiency of an apple CRISPR / Cas9 system by using an improved leaf disc conversion method, and belongs to the technical field of biology. The invention provides a culture medium composition according to the requirement of woody plant tissue culture nutrients, the requirement of apple leaf regeneration can be met, calluses and regenerated buds with high differentiation capacity are induced, and the problem of low genetic transformation efficiency of apples is solved. An improved leaf disc conversion method based on the culture medium package can significantly improve the editing efficiency of an apple CRISPR / Cas9 system, can efficiently deliver a CRISPR / Cas9 knockout carrier, obtains homozygote and heterozygote plants of different mutation types including deletion, replacement, insertion and the like, and has the editing efficiency as high as 92.3%. The method provided by the invention provides a new thought for analyzing genetic mechanisms of apples, improving agronomic characters and performing germplasm innovation by utilizing a gene editing technology.

The invention provides sgRNA and CRISPR / Cas9 vectors, a construction method of the sgRNA and CRISPR / Cas9 vectors and application of the sgRNA and CRISPR / Cas9 vectors in poplar gene editing, and relates to the technical field of plant genetic engineering. The specific sgRNA is obtained by optimizing the length and the repetition number of the sgRNA, the CRISPR / Cas9 system is constructed on the basis of the sgRNA, and the CRISPR / Cas9 system can remarkably improve the editing efficiency of the poplar endogenous genes PagRbcS and PagHEMA, and particularly improve the editing efficiency of the monoalleles and dialleles of the poplar endogenous genes.

The present application relates to the field of protein engineering, in particular to a fusion protein with an expanded PAM recognition range and application thereof. The fusion protein provided by the present application comprises: a) an amino acid sequence from a non-PI domain of xCas9; b) an amino acid sequence from a PI domain of a SpCas9 variant. The fusion protein has a more relaxed PAM restriction and significantly improved editing efficiency, and is a novel editing tool with an expanded targeting range.

The invention discloses a dynamic adjustment method for virtual reality fracturing field simulation. The method comprises the following steps: constructing a scene function setting component; constructing a placement scene function component, loading the placed fracturing equipment file by using the placement scene function component, obtaining the position of the fracturing equipment, dynamically adjusting the fracturing equipment, and storing the dynamically adjusted fracturing equipment file; constructing an observation scene function component, and based on the setting scene function component, the placing scene function component and the observation scene function component, adopting a UGUI framework in Unity to construct a virtual reality fracturing field simulation user interface; the invention relates to a virtual reality fracturing field simulated user interface. According to the method, dynamic adjustment of virtual reality fracturing field simulation can be achieved, changes of models and positions of different fracturing field devices are reflected in a model scene, the sense of substitution of operators is enhanced, and the editing efficiency of the fracturing devices can be improved.

This application discloses a view editing method, device, storage medium, and program product, relating to the field of smart home / intelligent home technology. The view editing method includes: determining the target snapping position of the target object in the floor plan based on the movement position of the target object and the floor plan; the movement position is generated by the user triggering the movement of the target object in the view editing interface; the target object includes: a device model or a furniture model; obtaining a target space view based on the floor plan, the target snapping position, and the target object; in the target space view, the target object is displayed at the target snapping position of the floor plan; this method adopts a drag-and-drop editing method, which is simple to operate, improves editing efficiency, and effectively ensures the geometric accuracy of the target object's spatial placement and the rationality of the view layout through automatic snapping.

This invention involves co-transfecting cells with an AAV vector plasmid carrying the target gene, a packaging plasmid providing Rep / Cap proteins, and a helper plasmid providing adenovirus assistance. The transfected cells are cultured under suitable conditions to produce viral particles containing recombinant AAV. Cells and culture supernatant are collected, cells are lysed to release the virus, and the viral particles are purified by centrifugation. A membrane-penetrating peptides or their derivatives are then modified onto the AAV capsid protein. Results show that the gene editing system combined with membrane-penetrating peptides or their derivatives enhances editing efficiency, effectively penetrates ocular surface tissues, and inhibits ocular neovascularization.

The present application discloses a method, device, equipment and storage medium for real-time preview editing of a web page, the method comprising the following steps: creating a blank template at a web page editing end, inserting the HTML source code of a published web page obtained from a remote server into the blank template; after monitoring that the blank template completes the insertion of the HTML source code, instantiating the blank template to preview the corresponding editable web page at the web page editing end; receiving editing data acting on the editable web page, storing the editing data in a global variable; in response to the data update of the global variable, refreshing the preview effect of the editable web page. The present application is suitable for providing an efficient editing solution for large-scale web page special development projects, and can better achieve code isolation and management.

The present disclosure provides a method, device, electronic device and storage medium for editing multimedia resources, and belongs to the field of multimedia technology. The method includes: displaying the target multimedia resources to be edited and the recipe control in the resource editing interface, the recipe control is used to provide an editing recipe for the target multimedia resources, and the editing recipe is generated based on the filter indicated by the filter application operation and the resource processing parameters indicated by the adjustment operation in the historical time period; in response to the triggering operation of the recipe control, at least one editing recipe is displayed in the resource editing interface; in response to the selection operation of any editing recipe, the target multimedia resources are edited based on the filters and resource processing parameters in the editing recipe. The above method can directly edit the target multimedia resources according to the filters and resource processing parameters previously used by the user, which not only meets the user's needs, but also is simple to operate, thereby improving the editing efficiency.

This application belongs to the field of biotechnology and relates to an efficient and specific editing system, method, and application for A-G base substitution. The fusion protein described in this application comprises, from the N-terminus to the C-terminus, a first SaCas9 nickase fragment, a chimeric deaminase fragment, and a second SaCas9 nickase fragment. The deaminase is selected from adenosine deaminase or a variant thereof, specifically ecTadA8e. The amino acid sequence of ecTadA8e is shown in SEQ ID NO. 20, or has more than 80% sequence identity with the amino acid sequence shown in SEQ ID NO. 20, and possesses the function or activity of ecTadA8e. The fusion protein provided in this application, combined with the corresponding guide RNA, can efficiently and specifically replace the base A with G at the target site, providing an effective tool for repairing pathogenic mutations, studying gene function, and improving cell function, and has promising application prospects.

The invention belongs to the field of gene engineering. Specifically, the invention relates to a modular gene editing tool and application thereof. Specifically, the invention provides a modularized gene editing tool protein capable of accurately editing a genome nucleotide sequence of an organism, a gene editing system comprising the protein and a method for performing gene editing by using the system. The modular gene editing tool disclosed by the invention can realize efficient, accurate and universal gene editing in cells of various eukaryotes such as animals and plants. The types of existing editing tools are enriched, and the method has a wide application prospect.

The present invention relates to the technical field of three-dimensional scene editing, and discloses a three-dimensional scene editing method, device, equipment and storage medium, which converts the natural language editing instructions input by the target editing user into step instructions and operation instructions, extracts parameter index characterization information, generates operation parameters corresponding to the operation instructions under the step instructions based on the operation parameter standard model of the target editing user, and finally constructs a three-dimensional scene based on the operation instructions and operation parameters corresponding to each step instruction. The present invention uses a large language model to replace the three-dimensional editor with a three-dimensional scene editing framework based on natural language control, converts the natural language editing instructions input by the user into the operation parameters of the corresponding operation instructions under each step instruction, introduces the operation parameter standard model to record the parameter index characterization habit characteristics of each user, improves the efficiency and flexibility of three-dimensional scene editing, and reduces the threshold of three-dimensional scene editing operations.

The application discloses a method for creating non-transgenic mutant strains of Chinese cabbage. The method for creating non-transgenic mutant strains of Chinese cabbage provided by the application is realized by combining CRISPR / Cas9 technology and Chinese cabbage in situ transformation technology, and specifically comprises the following steps: introducing a CRIPSR / Cas9 gene editing vector carrying an sgRNA coding sequence of a target gene in a Chinese cabbage genome into a receptor Chinese cabbage by a vacuum infiltration in situ transformation method. Two PDS allele knockouts are obtained in 2032 seeds, and the non-transgenic editing mutant strains are obtained by the in situ transformation technology which is independent of tissue culture. The editing efficiency is greatly improved compared with the previously reported in situ transgenic efficiency, and the application provides a good research idea for transgenic safety and marker-free gene editing.

The embodiment of the invention discloses a virtual scene editing method and device, computer equipment and a storage medium. According to the scheme, the game editing scene is displayed through the graphical user interface, and the game editing scene is configured to generate the corresponding game operation scene in the game operation stage; in response to the scene editing instruction, displaying a scene environment editing page corresponding to the target virtual object; in response to a scene editing operation for the scene environment editing page, determining environment special effect information corresponding to the target virtual object; in response to the game operation instruction, generating a game operation scene corresponding to the game editing scene; and according to the position of the target virtual object and the environment special effect information, rendering the environment special effect in the game operation scene. Therefore, by setting the display range of the environment special effect corresponding to the virtual object, the display area of the environment special effect can be controlled according to the position of the virtual object in the virtual scene, the operation is convenient, and the editing efficiency of the virtual scene can be improved.

The invention relates to an image local editing method and device, a readable storage medium and a program product. The method comprises the steps of obtaining a to-be-edited image, wherein the to-be-edited image comprises an editable area and a content retention area; obtaining a description text for the editable area; performing down-sampling on the to-be-edited image to obtain a down-sampled image; performing image generation according to the down-sampling image and the description text to obtain an intermediate image; the content of an image block corresponding to the position of the editable region in the intermediate image is matched with the description text; performing up-sampling on the intermediate image to the image size of the to-be-edited image to obtain an up-sampled image; and fusing an image block corresponding to the position of the editable region in the up-sampling image with the content retention region to obtain a target image. By adopting the method, the image local editing efficiency can be improved.

The invention discloses a model training method and device, an image processing method and device and electronic equipment, and belongs to the technical field of image processing. The method comprises the steps that a first training sample set is acquired, the first training sample set comprises multiple groups of training samples, and one group of training samples comprises a first face sample image, a first face reference image and sample editing information, editing the first face sample image based on the sample editing information to obtain the first face reference image; inputting the first training sample set into a first image processing model to obtain a first predicted face image output by the first image processing model, the first image processing model comprising a low-rank adaptive model; and according to the first predicted face image and the first face reference image, adjusting model parameters of the first image processing model to obtain a second image processing model.

The present application relates to the technical field of biotechnology, and particularly relates to a CRISPR / SuCas12a2-based gene editing system and application thereof. The present application constructs a high-efficiency counter-selection recombination editing tool by exploring the counter-selection potential of CRISPR-SuCas12a2 and combining it with lambda recombinase. The method can complete high-efficiency recombination editing of genes through a double-plasmid and single-plasmid system carrying SuCas12a2 and lambda recombinase, and the efficiency is greatly improved compared with the current recombination editing method based on lambda recombinase. Compared with the previous dependence on the specific cleavage of target double-stranded DNA by CRISPR-Cas protein to promote the effect of recombination editing, the efficiency is also obviously improved, and the method has the advantages of more transformants and low probability of immune escape. The plasmid construction, recombination editing and plasmid elimination operations can be completed within one week. The above functions supplement a powerful tool for gene recombination editing.

This invention discloses the application of chloroquine in improving gene editing efficiency. Specifically, it provides a novel use for chloroquine: enhancing the gene editing efficiency of gene editing reagents on recipient biological materials. The gene editing reagent is a gene editing tool plasmid. No prior art studies have combined chloroquine with gene editing efficiency. The inventors of this invention have discovered that treating recipient biological materials with chloroquine before transfecting them with gene editing reagents can significantly improve gene editing efficiency. This invention is simple to operate, widely applicable, and opens up a new avenue for improving editing efficiency by introducing exogenous small molecules rather than directly modifying gene editing tools.

The invention discloses application of hydroxychloroquine to improvement of gene editing efficiency. The invention provides novel application of hydroxychloroquine, namely application of hydroxychloroquine in improving gene editing efficiency of a gene editing reagent on a receptor biological material. In the prior art, a research of combining hydroxychloroquine and gene editing efficiency is not seen. The inventor of the invention finds that the gene editing efficiency can be remarkably improved by treating a receptor biological material with hydroxychloroquine and then transfecting a gene editing reagent. The method is easy and convenient to operate and wide in applicability, and a new way for improving the editing efficiency is developed by introducing exogenous small molecules instead of directly modifying a gene editing tool. The invention provides a basis for exploring an editing efficiency improvement strategy by adding exogenous molecules instead of modifying an editor mutant.

The present application relates to the field of genetic engineering, in particular to a pig cell model of NF2 gene mutation and a construction method and application thereof. The construction method comprises the following steps: 1) designing and synthesizing pegRNA and Nick sgRNA according to the pig NF2 gene; 2) constructing a vector: taking an sgRNA expression plasmid as a skeleton, inserting pegRNA into a BbsI enzyme cutting site of the plasmid to construct a pCL108PE1 vector; taking the sgRNA expression plasmid as the skeleton, inserting Nick sgRNA into the BbsI enzyme cutting site of the plasmid to construct a pCL108NICK1 vector; 3) electrotransferring cells: simultaneously electrotransferring PE protease plasmid, the pCL108PE1 vector and the pCL108NICK1 vector into pig cells; 4) obtaining the pig cell model of NF2 gene mutation: culturing the electrotransferred pig cells, screening out positive clone cells and obtaining the pig cell model of NF2 gene mutation.

The invention relates to a frame selection interaction-based video element elimination method and device, equipment and a medium, and the method comprises the steps: responding to a frame selection operation of a user in a video preview region, generating a rectangular selection region covering a target elimination element, and obtaining the coordinate information of the rectangular selection region in an original video frame coordinate system; generating a binary mask image with the same resolution as the original video frame in the client based on the coordinate information; sending the original video frame and the binary mask image to a cloud server; performing content restoration on a corresponding region in the original video frame based on the binary mask image through an image elimination model to generate elimination result data; and returning the elimination result data to the client, and rendering and displaying the elimination result data in the video preview area to replace the original picture. According to the method, the target element area is accurately specified through the frame selection interaction mode, mask defects are reduced, the content repairing quality is improved, and meanwhile operation stability and editing efficiency are improved.

This invention relates to the field of gene editing technology, specifically to an Mb4Cas12a mutant protein, a CRISPR / Cas12a gene editing system using this mutant protein, and its applications. The complex formed by the Mb4Cas12a mutant protein and crRNA of this invention can precisely target DNA sequences and induce cleavage, causing double-strand breaks in the target sequence. It exhibits high specificity, reduces off-target rates in gene editing performed in cells or in vitro, and can distinguish single-base differences at target sites, thus possessing broad application prospects.

The invention provides a video dubbing method and device, computer equipment and a storage medium, and the method comprises the steps: obtaining a video to be dubbed with music, and obtaining the video beat data, video emotion data and video content data of the video to be dubbed with music according to the video to be dubbed with music; obtaining a plurality of pieces of music to be matched, obtaining music beat data, music style data and music content data of each piece of music to be matched according to the plurality of pieces of music to be matched, and comparing the music beat data, the music style data and the music content data with video beat data, video emotion data and video content data corresponding to the video to be matched with music; and according to a comparison result, determining candidate matching music from the plurality of pieces of to-be-matched music. According to the invention, music matching can be rapidly carried out on the video, and the video music matching efficiency is effectively improved.