Experimental method for constructing Hutat2:Fc gene knock-in monocyte by using CRISPR/Cas9 technology
A monocyte and gene knock-in technology, applied in the medical field, can solve the problems of difficult operation of gene-directed insertion technology, unable to express genes stably for a long time, affecting the stability of the genome, etc., to protect the migration ability, wide application range, increase effect of possibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0042]CRISPR / Cas9 technology is a newly discovered gene editing technology in recent years, which can realize gene editing at specific sites in the genome. Compared with ZFN / TALEN-mediated gene-directed insertion technology, CRISPR / Cas9 technology is easy to operate and has high editing efficiency. It can realize site-directed single-copy gene insertion and has little impact on genome stability. Moreover, the inherent AAVS1 site in the human genome sequence is a "safe harbor" site in the human genome sequence, which can realize the insertion of various foreign genes without affecting the normal function of cells. Therefore, the present invention uses this technology to insert the therapeutic gene into the AAVS1 site of human primary mononuclear cells, so that it can pass through the blood-brain barrier and enter the nervous system to exert a therapeutic effect.
[0043] Utilize CRISPR / Cas9 technology to construct Hutat2: the experimental method of Fc gene knocking into monocyt...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com