Method for specifically improving gene editing efficiency of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-CAS system in epidermal stem cell

An epidermal stem cell and gene editing technology, applied in the field of improving the gene editing efficiency of CRISPR-cas system in epidermal stem cells, can solve the problem of low homologous recombination probability, and achieve the effect of improving gene editing efficiency and efficiency
CN108610399AActive Publication Date: 2018-10-02河北万玛生物医药有限公司
5 Cites 23 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
河北万玛生物医药有限公司
Publication Date
2018-10-02

Smart Images

  • Figure 1
    Figure 1
Patent Text Reader

Abstract

The invention provides some new enhancins ESCS-higher, which can obviously improve the gene editing efficiency of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas9 in an epidermal stem cell. The invention provides a new way to improve the gene editing efficiency in the cell, and further provides a more efficient genome editing system. When the enhancin is used with the CRISPR / Cas9, the genome editing efficiency of the CRISPR / Cas9 in the epidermal stem cell can be obviously improved.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention provides a method for improving the gene editing efficiency of the CRISPR-cas system in epidermal stem cells, and in particular relates to a new synergistic factor that can significantly increase the probability of homologous recombination, combining the synergistic factor with the CRISPR-cas system Can significantly improve the efficiency of genome editing. Background technique

[0002] Epidermal stem cells (Epidermal stem cells, EpiSCS) are stem cells with self-proliferation and multi-lineage differentiation potential. Its normal proliferation and differentiation are the basic requirements for maintaining the structural and functional integrity of the skin and its appendages (sweat glands, hair, sebaceous glands). Under physiological conditions, epidermal stem cells differentiate into a stem cell and a transit amplifying cell (TA cell) through asymmetric division, and the TA cell differentiates into post-mitotic cells (Post-mito...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More