44 results about "Gene synthesis" patented technology

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention discloses an inflorescence gene silencing method which comprises the following steps: firstly, synthesizing a specific nucleotide fragment according to a target gene, and constructing the specific nucleotide fragment to a plant virus vector; introducing the constructed plant virus vector and the corresponding helper plasmid into the inflorescence; soaking the inflorescence in a metal chelating agent salt solution; and finally, screening to obtain plants with inflorescences subjected to target gene silencing. The chrysanthemum capitulum is selected as an infection object, the silencing method is optimized, the target gene silencing efficiency is remarkably improved, the phenotype detection period after infection is remarkably shortened, and the method is efficient, convenient and high in operability. Meanwhile, the method can effectively avoid the cross protection phenomenon of plants, overcomes the obstacle of limited transmission range of viral vectors, does not need to consider the problems of death and growth cycle of tissue culture seedlings or seedlings after infection, and provides a feasible means for rapid identification of chrysanthemum gene functions and large-scale development of chrysanthemum gene function research.

This invention provides a method and kit for enhancing the yield and quality of gene synthesis products, comprising the following steps: providing a total primer mixture, which includes multiple oligonucleotide single strands encoding a target gene sequence / encoding an operational sequence and sterile water; using the primer mixture as a template, performing the first... PCR The reaction yielded the primary spliced ​​product; a second reaction was then performed using start and end primers. PCR The reaction yielded the crude gene synthesis product; nested primers were then used for a third reaction. PCR The reaction yields a nested structure. PCR Products; nested PCR The product was subjected to agarose gel electrophoresis, and the target band was excised and recovered. This application improves upon traditional gene synthesis methods by adding operation sequences to both ends of the synthesized gene sequence and utilizing highly specific nested primers for nested gene synthesis. PCR It can synthesize more than 2000bp in one go. DNA Double-stranded sequences significantly improve the yield and quality of gene synthesis products.

The invention relates to a bemisia tabaci gene BtCOX8 and application thereof, and belongs to the field of biological control. The gene BtCOX8 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtCOX8 is a potential efficient RNAi lethal target. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa and orius minutus which are natural enemies of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a good application prospect.

This invention relates to plant genetic engineering, and more particularly to a variegated Phalaenopsis orchid and its preparation method, the preparation method comprising the following steps: (1) according to the target gene with the base sequence shown in SEQ ID No. 1 PePDS (1) Synthesize specific nucleotide fragments; (2) Construct specific nucleotide fragments into a plant virus vector; (3) Introduce the recombinant plant virus vector obtained in step (2) into Phalaenopsis orchid plant material; (4) Screen to obtain Phalaenopsis orchids with silenced target genes. This invention achieves rapid, efficient, and non-transgenic leaf color variegation creation in commercial Phalaenopsis orchid varieties for the first time through VIGS technology; it not only breaks through the technical bottleneck of difficult and long cycle of genetic manipulation of orchid plants, significantly shortening the trait verification cycle, but also induces diverse and highly ornamental variegated leaf phenotypes such as albino, yellow, or purple in different varieties, and has the advantages of simple operation, low cost, and industrialization feasibility.

The invention discloses a teneplase mRNA (messenger Ribonucleic Acid) medicine and application thereof. The active ingredient of the medicine provided by the invention is mRNA (messenger ribonucleic acid) as shown in SEQ ID NO.5. The invention also protects a recombinant plasmid of the teneplase as shown in SEQ ID NO. 4. Specific DNA molecules are obtained through a series of design and sequence optimization, specific recombinant plasmids are synthesized through gene synthesis, the specific recombinant plasmids are subjected to in-vitro transcription, cell supernatant protein is obtained through transfection of mRNA of different amounts at different time points after transfection, and the cell supernatant protein is used for preparing the recombinant protein. According to the present invention, it is detected through ELISA that the tinipase mRNA can be effectively expressed in different cells of different species, such that the pioneering significance is provided for the development of the tinipase mRNA drug.

The invention relates to the technical field of recombinant protein, in particular to self-assembled calcium chelate keratin RK35DE and a preparation method thereof. According to the invention, the self-assembled calcium-chelated keratin RK35DE is obtained through protein design, whole-gene synthesis, vector construction and escherichia coli expression. The keratin RK35DE is subjected to self-assembly research, and the TEM image of the keratin RK35DE shows that the keratin RK35DE has self-assembly capability. In a calcium chelating ability experiment, compared with that before recombination, the self-assembled calcium-chelated keratin RK35DE disclosed by the invention has a remarkable chelating effect on calcium ions. In the prior art, keratin with self-assembly capability lacks effective coordination capability of forming stable chelates with calcium ions in hydroxyapatite, so that stable compounds are difficult to form and exert effects. The self-assembled calcium chelate keratin researched and developed by the scheme provides a new thought for researching a new hydroxyapatite tooth restoration material, and has an ideal popularization and application prospect.

This invention discloses a method for constructing a multi-target amplicon abundance standard for microorganisms and its application, belonging to the fields of molecular biology detection and microbiome analysis. This standard material screens sequences of common human gut microbiota strains, designs and adds universal primers, and obtains 14 DNA fragments of different lengths through gene synthesis, cloning, and purification, which are then mixed according to a preset abundance. It retains natural characteristics such as primer binding sites and GC content, and can systematically correct technical deviations in amplicon sequencing, solving the problem of species abundance distortion. This standard material functions as both a non-homologous internal reference and a homologous external reference, and can be used for laboratory proficiency testing, reagent kit performance evaluation, and cross-platform data calibration, promoting the leap from relative qualitative to absolute quantitative research in microbiome studies and providing metrological support for the standardization and precision of detection results.

The invention discloses a sulfanilamide drug-resistant gene standard substance as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) gene synthesis and clone construction; (2) preparing plasmid DNA (deoxyribonucleic acid); (3) DNA standard substance identification, subpackaging and storage; and (4) determining the value of the standard substance. According to the method, two valuing methods with different principles are adopted, a multi-step standard substance candidate purification method is developed by researching factors influencing the consistency of quantitative results between the two valuing methods, and impurities influencing the valuing results are removed. By optimizing preparation and valuing of the standard substance, equivalence and consistency of two valuing methods with different principles are achieved, and the problem that valuing can only be carried out through cooperation of multiple laboratories is solved.

The application discloses a method for constructing an animal model infected with hepatitis A virus and application thereof. Specifically disclosed is a method for constructing an animal model infected with hepatitis A virus, which comprises the following steps: encapsulating a complete genome RNA of hepatitis A virus in LNP to obtain LNP-encapsulated hepatitis A virus genome RNA; inoculating the LNP-encapsulated hepatitis A virus genome RNA into a non-human mammal to obtain the animal model infected with the hepatitis A virus. The LNP-encapsulated HAV genome RNA is obtained through gene synthesis, and is used as inoculating material of the animal infection model, so that the LNP-encapsulated HAV genome RNA is convenient to obtain and has good uniformity and repeatability. The application first establishes an animal model infected with hepatitis A virus without isolation and culture of the virus, and the virus can be directly established and amplified, and compared with a strain, the virus genome RNA is easier to obtain and safer to operate.

The application belongs to the technical field of genetic engineering, and particularly relates to a CsECT protein related to the disease resistance of plants, a recombinant vector and application thereof. 6 The CsECT protein provided by the application is synthesized by the gene CsECT, and the gene CsECT is speculated to be an m A reader, which affects the metabolism of target mRNAs, improves the stability of disease resistance related target genes, promotes the expression of disease resistance genes, and further improves the disease resistance of plants. The results of the examples show that the CsECT gene is cloned to construct a CsECT transgenic citrus, the transgenic citrus overexpresses the CsECT gene, and the disease resistance of the citrus to bacterial wilt can be significantly improved, thereby improving the disease resistance of the citrus. It can be seen that the CsECT protein synthesized by the gene CsECT can improve the disease resistance of plants.

The application discloses a tobacco protein disulfide isomerase NbPDI and application thereof in regulation of plant immunity. The application obtains an anti-disease related gene NbPDI01 and a susceptible gene NbPDI23 from tobacco. NbPDI01 and NbPDI23 are overexpressed in tobacco through a transient expression technology, and pepper Phytophthora is inoculated after 2 days. The results show that, compared with a negative control, the disease spot area of the tobacco leaf expressing NbPDI01 is significantly reduced, while the disease spot area of the tobacco leaf expressing NbPDI23 is significantly increased, and biological statistics analysis shows that the difference between the two is significant. It is shown that NbPDI01 can specifically improve the disease resistance of tobacco, and therefore, the NbPDI01 gene provided by the application can be applied to the fields of plant disease resistance breeding, disease resistance gene synthesis and genetic engineering improvement, and provides a new gene resource for improving the disease resistance of crops, and has important theoretical significance and application prospect.

The utility model provides a kind of preparation device of fixed phase chip for enzyme method gene synthesis, belong to enzymatic method gene synthesis technical field, including shell, chip fixing frame and upper cover, shell top is open and inside forms chip reaction pool, shell side portion has liquid inlet hole and liquid outlet hole, liquid inlet hole and liquid outlet hole are respectively used to pass pipeline communication peristaltic pump, peristaltic pump is used to push reaction liquid flow through chip reaction pool and form circulating flow;Chip fixing frame is placed in the inside of shell chip reaction pool, and chip fixing frame has multiple chip fixing slots suitable for accommodating multiple phase chips;Upper cover is sealingly connected to the top of shell and is suitable for covering top opening, so that chip reaction pool forms closed space.The preparation device of fixed phase chip for enzyme method gene synthesis provided by the utility model can produce medium batch chips, meet the use, improve yield and save cost.

The application discloses a method for rapidly and non-traceably preparing mRNA containing long polyadenine and application thereof. Through modular design and an IIS type restriction DNA endonuclease system, complex gene synthesis containing long poly(A) is decomposed into two steps of rapid synthesis of simple target genes and non-traceable connection with a previously prepared leading plasmid. The mRNA synthesized based on the method can carry a fixed length of poly(A) without tailing operation, and since the target gene synthesis does not involve a long poly(A) complex fragment, the speed and efficiency of the template plasmid preparation are greatly improved. The plasmid can be directly used as a linearized template for in vitro transcription synthesis of long polyadenosine deoxyribonucleic acid poly(A) mRNA, the IIS type restriction DNA endonuclease system does not need to introduce an additional splicing sequence between the target gene and poly(A), and the transcribed mRNA sequence completely meets the expectation.

The application belongs to but is not limited to the technical field of gene synthesis, and discloses a preparation method of a red blood cell membrane coated framework nucleic acid nanogel, which forms NG through base complementary pairing, and realizes the compounding of RBCm and NG by using an Avanti small extruder.SiRNA is combined and cross-linked with TDN through base complementary to form NG, and the TDN provides the first layer of protection for the siRNA, enhances the stability and drug efficacy of the siRNA, and the red blood cell membrane provides the second layer of protection to prevent enzyme degradation and protein adsorption.M@NG combines the bionics and biocompatibility of RBCm and the high drug loading and stability of NG, and has the characteristics of targeted drug delivery, immune escape and the like.The system is suitable for the treatment of various diseases, and expands the application field of RNA interference technology.

The invention provides an anti-eperythrozoon ovine HSP70 protein polyclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The polyclonal antibody disclosed by the invention is prepared by immunizing an animal with an immunogen containing 109th-266th amino acids of the eperythrozoon ovine HSP70 protein, and has the characteristics of high titer and strong specificity. The preparation method of the polyclonal antibody comprises the steps of gene synthesis, protein expression and purification, animal immunization, antibody purification and the like. The invention also provides an application of the polyclonal antibody in immunological detection such as Western Blot, ELISA (Enzyme-Linked Immunosorbent Assay) and the like, and a detection kit containing the polyclonal antibody. The polyclonal antibody provides a core reagent for serological diagnosis of sheep eperythrozoonosis, and has important application value.

This invention belongs to the field of microbial technology, specifically relating to a Brucella-deficient strain 104MΔBioZ, its construction method, and its applications. The Brucella-deficient strain 104MΔBioZ is formed by knocking out a gene involved in biotin synthesis in the Brucella 104M genome. BioZ The strain obtained after gene sequencing; the strain involved in biotin synthesis BioZ The nucleotide sequence of the gene is shown in SEQ ID NO:1. This invention utilizes the knockout of biotin synthesis in Brucella 104M. BioZ A mutant strain was obtained through gene synthesis. The resulting mutant strain exhibited significantly reduced virulence compared to Brucella 104M and demonstrated highly effective resistance to infection by the parent Brucella. Results showed that immunizing healthy mice with the 104MΔBioZ strain described in this invention for brucellosis prevention is safe and provides high protection. The Brucella-deficient strain 104MΔBioZ has broad application prospects in the field of brucellosis prevention and control.

The application belongs to the technical field of biological enzymes, and particularly relates to an expression and purification method of a chitin synthase from a virus and application thereof. The expression and purification method of the chitin synthase from the virus comprises the following steps: a chitin synthase gene sequence from a virus is codon-optimized according to the codon preference of Escherichia coli, so that a nucleotide sequence as shown in the gene sequence of SEQ ID NO. 1 is obtained; the optimized gene sequence is synthesized into an expression vector; a host containing the expression vector is cultured, induced, lysed and centrifuged, so that a membrane component containing the chitin synthase from the virus is obtained. The application provides a purification method of the chitin synthase from the virus, utilizes the strategy of heterologous expression of Escherichia coli and separation and purification of a biomembrane-like system, and through the strategy, the chitin synthase from the virus with high purity can be obtained. A new method for synthesizing oligochitooligosaccharide is provided, and the reaction system is simple in composition, and the product is easy to separate and purify.

The invention discloses a recombinant rabies virus as well as a construction method and application thereof, and belongs to the technical field of reverse genetics. The technical problem to be solved is to provide a construction method of a recombinant rabies virus with a visual virus position and to realize efficient packaging of the recombinant virus, and the key point of the technical scheme is that the construction method of the recombinant rabies virus comprises the following steps: S1, constructing a recombinant plasmid containing recombinant rabies virus cDNA through whole-genome synthesis; s2, respectively amplifying N, P, M, G and L genes from the recombinant plasmid, and respectively constructing auxiliary plasmids containing the N, P, M, G and L genes; s3, transfecting host cells with the recombinant plasmids and helper plasmids corresponding to the N, P, M, G and L genes according to the mass ratio of 5: (0.2-1): (0.2-1): (0.2-1): (0.2-1): (0.2-1): (0.2-1) to obtain the recombinant rabies virus.

The invention relates to methods and compositions useful for routing and tracking multiple mobile units within a microfluidic device. Mobile units may be routed through a plurality of chemical environments, and the mobile units may be tracked to determine the path and / or environments that the mobile units have routed through. Mobile units may be routed in accordance with a predetermined algorithm. Mobile units may be routed through microfluidic devices in ordered flow. Mobile units routed through the microfluidic device can be used to perform various chemical reactions uniquely associated to the units, including without limitation peptide synthesis, enzymatic gene synthesis and gene assembly.

The application discloses a novel coordination peptide QEY127 and application thereof in assembling a luminescent material with a rare earth ion. The coordination peptide QEY127 is composed of one start codon coding methionine (M), one 6-polyhistidine tag (6X His) and 10 QSEPGDPGEPSY (QEY12), and has a length of 127 amino acid residues; the coordination peptide QEY127 gene is obtained through a whole gene synthesis method, and the codon composition is optimized. The yield of the coordination peptide QEY127 is 100-1200 mg / L of fermentation liquor, and the purity is 82-95%. The coordination peptide QEY127 has the characteristics of glutamine transaminase (TG enzyme) catalyzed crosslinking, high elasticity and formation of a luminescent coordination compound with a rare earth metal, and has a wide application prospect in the fields of biological medical treatment and luminescent materials.

The invention discloses preparation and application of PT-IRAKH-1 anti-inflammatory recombinant protein, and relates to the technical field of medicinal chemistry, and the key points of the technical scheme are as follows: a PT-IRAKH-1 active peptide coding sequence is a sequence which is derived from circular RNA (Ribonucleic Acid) and has coding potential, the size of the PT-IRAKH-1 active peptide coding sequence is 324 basic groups, and the nucleotide sequence of the PT-IRAKH-1 active peptide coding sequence is shown as SEQ ID NO: 1. A section of sequence with polypeptide coding potential is found in circular RNA, plasmids for expressing the polypeptide sequence are constructed through gene synthesis and plasmids, the protein is successfully expressed through a prokaryotic expression system to be prepared into recombinant protein, meanwhile, a lipopolysaccharide-induced mouse lung tissue inflammation model is constructed, and the mouse lung tissue inflammation model can be used for treating the lung tissue inflammation. The PT-IRAKH-1 recombinant protein is applied to the model, and evaluation is performed through methods of HE dyeing, lung tissue Smith scoring, flow cytometry and the like to obtain a conclusion that the PT-IRAKH-1 recombinant protein can relieve inflammation and injury of mouse lung tissue.

The application discloses a soluble expression vector of a P450 enzyme for producing indole-3-methylamine and a construction method and application thereof. The construction method is that a nucleotide sequence for coding a P450 enzyme tAMIS protein and a nucleic acid sequence for coding a NADPH-cytochrome reductase CPR protein are expressed in a vector, so that a soluble expression vector of a plant-derived P450 enzyme in Escherichia coli is obtained. The expression vector solves the problem that most plant-derived P450 enzymes cannot exhibit biological activity in the Escherichia coli, and compared with using plants or yeasts as hosts, the time consumed for expressing proteins is reduced. A method for detecting indole-3-methylamine synthesized by the Escherichia coli through an exogenous gene is provided.

The invention belongs to the technical field of gene engineering and protein modification, and discloses a high-thermal-stability series sweet protein mutant and a preparation method thereof, the method comprises the following steps: S1, gene construction and expression, whole-gene synthesis of mutant DNA, and conversion; s2, purification and characterization; and S3, an amplification process: under the condition suitable for expressing the sweet protein mutant, carrying out amplification culture on the recombinant microbial host cell, and then recovering the sweet protein mutant from the recombinant microbial host cell. The series of sweet protein mutants are all based on wild type sweet protein, substitution mutation occurs at one site or multiple sites in the wild type sweet protein, the denaturation rate of the wild type sweet protein is reduced, and the thermal stability of each mutant is higher than that of the wild type sweet protein. On the basis of protein structure characteristics constructed in a diversified mode and a synergistic mechanism, the denaturation rate is remarkably reduced, the HTST technology can be adapted, the industrial production condition is met, and the application field of the protein is greatly expanded.

The application belongs to the technical field of genetic engineering, and particularly relates to application of a transcription factor MdWRKY71 or a MdWRKY71 gene in promoting anthocyanin accumulation in plants, wherein an amino acid sequence of the transcription factor MdWRKY71 is shown as SEQ ID NO:1, and a nucleotide sequence of the MdWRKY71 gene is shown as SEQ ID NO:2. The transcription factor MdWRKY71 is synthesized by the MdWRKY71 gene, and it is proved by transgenic and functional identification that the transcription factor MdWRKY71 synthesized by the MdWRKY71 gene as a new transcription factor for regulating anthocyanin accumulation in apples can positively regulate expression of key anthocyanin synthesis enzyme genes MdANS and MdDFR, and promote 5-amino levulinic acid (ALA)-induced anthocyanin accumulation in apples, and can play an important role in agricultural and forestry production and scientific research.

The application belongs to the technical field of biological medicine, and particularly relates to a circular RNA vaccine against porcine epidemic diarrhea virus and a construction method and application thereof. The circular PEDV-COE1 RNA vaccine is obtained through gene synthesis, in-vitro transcription, circularization and liposome nanoparticle wrapping. The DNA molecule, the circular RNA molecule, the protein sequence and the gene sequence involved in the application can be used for preparing various porcine diarrhea virus vaccines. After porcine epidemic diarrhea virus circular RNA is injected into the muscle of a pig, a high level of porcine epidemic diarrhea virus neutralizing antibody can be generated in the pig, and the occurrence of piglet diarrhea can be effectively reduced, so that the application has great popularization value for the prevention and control of porcine epidemic diarrhea.

The application discloses a construction method and application of an animal model infected with hepatitis E virus. Specifically disclosed is a construction method of an animal model infected with hepatitis E virus, which comprises the following steps: encapsulating complete genome RNA of hepatitis E virus in LNP to obtain LNP-encapsulated hepatitis E virus genome RNA; inoculating the LNP-encapsulated hepatitis E virus genome RNA into a non-human mammal to obtain an animal model infected with hepatitis E virus. The RNA dosage is as low as 1 ug, and the animal infection model can be established. The application solves the technical barrier that the hepatitis E virus is not suitable for in-vitro culture, and the virus does not need to be isolated from nature or clinical specimens. The LNP-encapsulated HEV genome RNA can be obtained through gene synthesis, and is used as an inoculation material of the animal infection model, so that the LNP-encapsulated HEV genome RNA is convenient to obtain, has good uniformity and repeatability, and is safer to operate.

The invention discloses targeted selenium-coupled anti-inflammatory fusion protein and application thereof in acute coronary syndrome reperfusion injury repair, and belongs to the technical field of biomedicine. A GPIb-Annexin A1 fusion protein is constructed firstly, specifically, a human GPIb active fragment serving as an N-terminal targeting element and a human Annexin A1 active fragment serving as a C-terminal anti-inflammatory element are connected in series through a (Gly4Ser) 3 flexible linker, the fusion protein sequence is shown as SEQ ID NO: 3, and the GPIb-Annexin A1 fusion protein is obtained through gene synthesis, vector construction, prokaryotic expression and Ni-NTA purification; and chelating selenomethionine serving as a selenium source with the fusion protein according to the mass ratio of 3: 1 in a phosphate buffer solution with the pH value of 7.5, and dialyzing, freezing and drying to obtain the selenium chelate. Experiments prove that the selenium chelate can significantly improve the left ventricular ejection fraction and the left ventricular shortening fraction of MI / RI mice, reduce the myocardial infarction area, inhibit the expression of TNF-alpha, IL-1beta and IL-6, and reduce the long-term myocardial collagen volume fraction, and can be used for preparing acute coronary syndrome reperfusion injury repair drugs.

The invention relates to the field of PDRN preparation in the technical field of biology, in particular to a rapid preparation method of sequence-controllable PDRN. The invention discloses a rapid preparation method of sequence-controllable PDRN, which comprises the following steps: firstly, establishing a PDRN library, selecting a PDRN sequence from the library, performing gene synthesis, and connecting the PDRN sequence into a cloning vector; and carrying out amplification by using a Taq enzyme and a specific primer pair. By optimizing and adjusting a reaction system, microgram-level, milligram-level and gram-level amplification reactions are realized, and amplification can be completed within 4 hours. And then carrying out ultrafiltration, alcohol precipitation, drying and the like to obtain a PDRN product. The PDRN prepared by the method is single in length, controllable in sequence and high in base composition consistency, breaks through the tradition that the PDRN is a mixture, is high in safety, simple in production process and high in preparation speed, greatly improves the production efficiency, and can remarkably improve the economic benefit.

The invention belongs to the technical field of biological medicines, and relates to a monomeric protein and an application thereof in preparation of a medicament for treating cerebral ischemia. The monomeric protein is a monomeric protein Tat-Mo01 with a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID NO. 1. The recombinant monomeric protein Tat-Mo01 is obtained by the following steps: synthesizing a target protein coding sequence through a gene, carrying out PCR (Polymerase Chain Reaction) amplification, carrying out double enzyme digestion, connecting with a pcDNA3.4 vector to construct a recombinant plasmid, carrying out screening, sequencing and verification through escherichia coli JM108, transfecting CHO-S cells for expression, carrying out affinity purification through Protein A / G coupled magnetic beads, and adding guanidine hydrochloride. The invention also provides an application of the recombinant monomer protein Tat-Mo01 in preparation of an anti-cerebral ischemia drug.