148 results about "Gene synthesis" patented technology

The invention relates to the technical field of bioinformatics and computational biology, and provides an anti-cancer drug target screening method and system based on hypergraph-knowledge graph dual-channel, a hypergraph-knowledge graph dual-channel architecture is constructed through a feature extraction module, a self-adaptive multi-modal fusion module dynamically integrates multi-source heterogeneous data, and the anti-cancer drug target screening method and system based on the hypergraph-knowledge graph dual-channel architecture are obtained. The method comprises the following steps: adaptively fusing high-order interaction features and semantic association features of a gene, generating a high-quality negative sample by using a generative adversarial network to optimize a training process, finally optimizing feature representation through a discriminator, and calculating a synthetic lethal probability score between any gene pair. According to the application, firstly, the extracted multi-dimensional features are normalized through the feature extraction module, then the importance of different features is dynamically weighted based on the attention mechanism, finally, the synthesis lethal relationship prediction probability of the gene pair is output through the multi-layer sensor, and under the theoretical framework of the gene synthesis lethal effect, the synthesis lethal effect of the gene pair is predicted. And an innovative solution is provided for anti-cancer drug target screening.

The invention discloses a preparation method of a recombinant engineering bacterium for expressing XVII type collagen COL17A1, and relates to the technical field of gene engineering, in particular to a preparation method of a recombinant engineering bacterium for expressing XVII type collagen COL17A1, which comprises the following steps: gene synthesis and vector construction: synthesizing an optimized sequence SEQ ID No.1 of human source XVII type collagen COL17A1; adding proper restriction enzyme cutting sites at two ends of the sequence; the synthesized gene sequence is cloned to a pGAPZ A-alpha A vector, and the expression of the gene under the control of a GAP promoter is ensured. According to the preparation method of the recombinant engineering bacterium for expressing the XVII type collagen COL17A1, by using a GAP promoter, the constructed recombinant engineering bacterium can keep a stable expression level under different environmental conditions, the GAP promoter serving as a constitutive promoter can continuously and stably express recombinant protein in pichia pastoris, an inducer does not need to be added, the production process is simplified, and the production cost is reduced. The production cost is reduced, and the production efficiency is improved.

The invention discloses application of an OfTPS7 gene in synthesis of an important terpenoid aroma substance linalool and an oxide thereof, a plant overexpression vector containing the OfTPS7 gene is used for instantaneously converting OfTPS7 gene into OfTPS7 petals and detecting volatile matters of infected petals, compared with a control group, the expression quantity of OfTPS7 is increased by 3.13 times, the content of linalool is increased from 182.25 mu g / gFW to 1851.12 mu g / gFW, and the content of linalool is increased by 3.12 mu g / gFW. And the content of linalool oxide is increased from 452.09 [mu] g / gFW to 3072.41 [mu] g / gFW. The OfTPS7 gene overexpressed in the petals can significantly improve the content of linalool and oxides thereof in the petals, which proves that the OfTPS7 is the linalool synthetase gene and can be used for improving the natural aroma quality of osmanthus fragrans, breeding excellent varieties and the like in osmanthus fragrans genetic engineering.

The invention relates to the technical field of biology, in particular to a gene for increasing the content of resveratrol in grapes and application of the gene. The invention discloses a gene for improving the content of resveratrol in grapes. The DNA sequence of the gene is shown as SEQ ID NO1. The amino acid sequence of the gene synthetic protein disclosed by the invention is as shown in SEQ ID NO2, and the protein is named as VvFHY34D. According to the invention, when four amino acids S124, T128, S156 and T160 of the VvFHY3 are all replaced with aspartic acid to simulate phosphorylation activation, the VvSTS expression quantity in transient over-expression VvFHY34D grapes is higher, and more resveratrol can be synthesized.

The invention belongs to the technical field of genetic engineering, and particularly relates to a CsECT protein related to plant disease resistance, a recombinant vector and application of the CsECT protein. The invention provides a CsECT protein related to plant disease resistance, the CsECT protein is synthesized by gene CsECT coding, it is speculated that the gene CsECT is used as an m6A reader, metabolism of target mRNA of the gene CsECT is affected, the stability of disease resistance related target genes is improved, expression of disease resistance genes is promoted, and then the disease resistance of plants is improved. The result of the embodiment shows that after the CsECT gene is cloned, the CsECT transgenic citrus is constructed, the CsECT gene is excessively expressed by the transgenic citrus, the canker resistance of the citrus can be remarkably improved, and then the disease resistance of the citrus is improved. Therefore, the CsECT protein synthesized by the gene CsECT disclosed by the invention can be used for improving the disease resistance of plants.

The invention discloses an SaRNA vaccine for echinococcosis as well as a preparation method and application of the SaRNA vaccine. The preparation method of the SaRNA vaccine comprises the following steps: carrying out codon optimization on a modified target antigen protein through a genetic engineering technology, then assembling the modified target antigen protein with a self-replicating protein sequence, 5 'UTR, 3' UTR and Poly (A) tail, carrying out gene synthesis, then cloning the synthesized gene into a plasmid, and carrying out purification to obtain the SaRNA vaccine. The preparation method comprises the following steps: constructing recombinant plasmids, sequentially carrying out plasmid linearization, in-vitro transcription and purification on the constructed recombinant plasmids to prepare SaRNA molecules, and finally wrapping the SaRNA molecules in lipid nanoparticles to form the SaRNA vaccine for the echinococcosis. Experiments prove that the SaRNA vaccine can activate humoral immunity and cellular immunity of mice at the same time, high-level EG95 specific antibodies and cytokines can be generated through low-dose immunity, and the SaRNA vaccine has wide application prospects in the aspect of preventing and / or treating the echinococcosis.

The invention discloses an rhFN recombinant human chemical fibronectin process of a genetic engineering technology, and particularly relates to the technical field of genetic engineering, and the rhFN recombinant human chemical fibronectin process comprises target gene acquisition, gene cloning and vector construction, host cell selection and transformation, cell culture and screening, protein expression and optimization, and protein separation and purification. According to the rhFN recombinant human chemical fibronectin process of the genetic engineering technology provided by the invention, high-quality target genes are accurately obtained through technologies such as new-generation sequencing and gene synthesis, and the gene expression efficiency and stability are enhanced by virtue of optimized vector construction and regulatory elements; protein expression ability and specificity are improved in host cell selection and transformation, efficient culture and accurate screening are realized in cell culture and screening links, total yield is increased in a protein expression optimization link, purity and efficiency are improved in a protein separation and purification link, and product quality and safety are ensured through comprehensive quality control. The requirements in the fields of medical treatment and scientific research are expected to be better met.

The invention relates to a key gene GqBurscon for wing development of a Qinghai grassland moth and application thereof. The key gene GqBurscon for the wing development of the Qinghai grassland caterpillar has a nucleotide sequence as shown in SEQ ID NO: 1. According to the dsRNA synthesized by the key gene GqBurscon for wing development of the Qinghai grass caterpillar, one chain of the dsRNA is the same as the sequence of SEQ ID NO: 1, and the other chain of the dsRNA is reversely complementary with the sequence of SEQ ID NO: 1. The key gene GqBurscon for the wing development of the Qinghai grassland is used for synthesizing dsRNA, the synthesized dsRNA is used for carrying out RNA interference on a target gene, the gene transcription level is knocked down, the gene is effectively silenced in chest, abdomen, wings and wing base tissues of male adult Qinghai grassland, the length and width of the wings are correspondingly reduced, the development of the wings is limited, and the gene transcription level is reduced. The flying capability is influenced, and the death is caused.

The invention discloses an echinococcosis mRNA (messenger Ribonucleic Acid) vaccine as well as a preparation method and application thereof. The preparation method of the mRNA vaccine for the echinococcosis comprises the following steps: carrying out codon optimization on a modified target antigen protein, then assembling the modified target antigen protein with 5 'UTR, 3' UTR and Poly (A) tail, carrying out gene synthesis, then cloning the synthesized gene into a pUC57 plasmid, and sequentially carrying out plasmid linearization, in-vitro transcription and purification on the constructed recombinant plasmid to prepare an mRNA molecule, thereby obtaining the mRNA vaccine for the echinococcosis. Finally, mRNA molecules are wrapped in lipid nanoparticles through a microfluidic method to form the mRNA vaccine for the echinococcosis, and immune effect evaluation is carried out on the mRNA vaccine for the echinococcosis. Experiments prove that the prepared mRNA vaccine for the echinococcosis can activate humoral immunity and cellular immunity of mice at the same time, can provide an effective protection effect for the mice attacking insects, and has a wide application prospect in the aspect of preventing and / or treating the echinococcosis.

The present invention relates to RSV F protein mutants and their applications, belonging to the field of vaccines. Based on the amino acid sequence of wild-type RSV F protein, the present invention has carried out modifications in multiple aspects and in various ways, including disulfide bond mutations, proline mutations, cavity filling mutations, electrostatic mutations, p27 sequence modifications, and / or trimerization domain modifications, and designed a variety of RSV F protein mutants. Then, through gene synthesis, expression, and ELISA detection, the present invention tested the pre-fusion conformation (pre-F) ratio and stability under high and low temperature conditions of the RSV F protein mutants, and screened out several RSV F protein mutants with a relatively high pre-fusion conformation ratio and stable structure. Finally, candidate antigen representatives were selected for immunogenicity detection, which proved that they have good immunogenicity and can be used for the development of RSV vaccines, the preparation of RSV-specific antibodies, or RSV detection reagents.

Provided are an RNA vaccine for porcine epidemic diarrhea and a preparation method therefor. The RNA vaccine is obtained by means of gene synthesis, in-vitro transcription, and lipid nanoparticle encapsulation, which can rapidly respond to the currently prevalent new PEDV strains, reduces the research and production cycle of new vaccine development, and provides an effective technical approach for the prevention and control of newly mutated highly virulent strains. The prepared RNA vaccine for porcine epidemic diarrhea has high production efficiency, can induce relatively high levels of porcine epidemic diarrhea virus neutralizing antibodies and binding antibodies in vivo, effectively reducing the occurrence of diarrhea in piglets, and thus holds great promotional value for the prevention and control of porcine epidemic diarrhea.

The invention discloses a construction and identification method of an influenza B DNA vaccine, which comprises the following steps: step 1, downloading sequence information of an influenza B recommended strain B / Austria / 1359417 / 2021 in vaccine strain recommended components in 2022-2023, carrying out codon optimization on an HA gene of the strain, and then carrying out gene synthesis, and naming the gene as BHA; 2, highly conservative sequences of HA protein, M2e protein and NA protein are selected for series combination, gene synthesis is performed after codon optimization, and the gene is named as G3; 3, respectively extracting plasmid DNAs of the synthetic fragments for later use; 4, amplifying a target fragment by using a PCR technology so as to connect a carrier; and 5, carrying out a target gene and vector connection experiment. According to the present invention, the HA protein sequence of the DNA vaccine can be rapidly replaced by using the restriction enzyme cutting site, the preparation method is simple, the immunogenicity is good, the cross protection is good, the preparation cost is low, the preparation requirement of the large-scale influenza vaccine can be met, and the important scientific significance and the important social value are provided.

According to the RNA vaccine for porcine epidemic diarrhea and the preparation method of the RNA vaccine, the RNA vaccine is obtained through gene synthesis, in-vitro transcription and lipid nanoparticle wrapping, the current epidemic new PEDV strain can be rapidly dealt with, the research and development and production cycle of new vaccine development is shortened, and an effective technical means is provided for prevention and control of new variant virulent strains. The RNA vaccine for porcine epidemic diarrhea prepared by the invention is high in production efficiency, can generate a high-level porcine epidemic diarrhea virus neutralizing antibody and binding antibody in vivo, can effectively reduce the occurrence of piglet diarrhea, and has great popularization value for prevention and control of porcine epidemic diarrhea.

The invention discloses a method for interfering expression of a target gene of fall webworm, and belongs to the technical field of agricultural biology.The method comprises the steps that the target gene is screened according to transcriptome data of fall webworm, dsRNA of the target gene is synthesized, and the dsRNA is diluted into a solution with the concentration of 0.6 ng / mu L; fresh mulberry leaves are soaked in the dsRNA solution and refrigerated at 4 DEG C for 24 hours, so that the dsRNA solution is fully absorbed by the mulberry leaves; feeding the hyphantria cunea with the treated mulberry leaves for 7-10 days continuously, collecting larvae, detecting gene expression through qRT-PCR (quantitative real-time polymerase chain reaction) by taking the uninterfered larvae as a contrast, and calculating the interference efficiency of the mulberry leaves soaked with dsRNA (double-stranded ribonucleic acid) to the target gene of the hyphantria cunea; according to the method, the mulberry leaves are soaked in the low-concentration dsRNA solution, and the hyphantria cunea is fed, so that expression of target genes of the hyphantria cunea is remarkably reduced, tedious injection operation is omitted, additional damage to larvae is avoided, and the method has important significance on research on insect gene functions and development of a new RNAi-based prevention and control technology.

The invention discloses a new enzyme with acylsulfonate transferase activity or a mutant thereof and application thereof, and belongs to the field of enzyme engineering. On the basis of an NCBI (National Center of Biotechnology Information) database, a new enzyme sequence is mined through a bioinformatics means, a new enzyme is obtained through codon optimization, gene synthesis and cell expression, the activity of acylsulfonate transferase is measured, and a corresponding mutant is obtained by using a directed evolution method. Compared with reported mouse acyl sulfonate transferase (ASTIV), the enzyme sequence obtained in the invention has better catalytic activity in a regeneration system of PAPS, and provides a new choice for enzymatic synthesis of PAPS.

The invention discloses acyl sulfonic acid transferase and application thereof, and belongs to the field of enzyme engineering. On the basis of an NCBI (National Center of Biotechnology Information) database, a new enzyme sequence is mined through a bioinformatics means to obtain the acyl sulfonate transferase derived from Podarcismuris, a new enzyme is obtained through codon optimization, gene synthesis and cell expression, and the activity of the acyl sulfonate transferase is measured. Compared with reported mouse acyl sulfonate transferase, the acyl sulfonate transferase provided by the invention has better catalytic activity in a regeneration system of PAPS, and a new choice is provided for enzymatic synthesis of PAPS.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention relates to an RSV F protein mutant and application thereof, and belongs to the field of vaccines. On the basis of the amino acid sequence of wild RSV F protein, multiple aspects and multiple modes of modification are carried out, including disulfide bond mutation, proline mutation, cavity filling mutation, electrostatic mutation, p27 sequence modification and / or trimerization structural domain modification, and multiple RSV F protein mutants are designed. And then, through gene synthesis, expression and ELISA detection, the pre-conformation (pre-F) proportion of the RSV F protein mutants and the stability under high-temperature and low-temperature conditions are tested, and a plurality of RSV F protein mutants with relatively high pre-conformation proportion and stable structures are screened out. Finally, candidate antigen representatives are selected for immunogenicity detection, and it is proved that the candidate antigen representatives have good immunogenicity and can be used for developing RSV vaccines and preparing RSV specific antibodies or RSV detection reagents.

The invention discloses an inflorescence gene silencing method which comprises the following steps: firstly, synthesizing a specific nucleotide fragment according to a target gene, and constructing the specific nucleotide fragment to a plant virus vector; introducing the constructed plant virus vector and the corresponding helper plasmid into the inflorescence; soaking the inflorescence in a metal chelating agent salt solution; and finally, screening to obtain plants with inflorescences subjected to target gene silencing. The chrysanthemum capitulum is selected as an infection object, the silencing method is optimized, the target gene silencing efficiency is remarkably improved, the phenotype detection period after infection is remarkably shortened, and the method is efficient, convenient and high in operability. Meanwhile, the method can effectively avoid the cross protection phenomenon of plants, overcomes the obstacle of limited transmission range of viral vectors, does not need to consider the problems of death and growth cycle of tissue culture seedlings or seedlings after infection, and provides a feasible means for rapid identification of chrysanthemum gene functions and large-scale development of chrysanthemum gene function research.

This invention provides a method and kit for enhancing the yield and quality of gene synthesis products, comprising the following steps: providing a total primer mixture, which includes multiple oligonucleotide single strands encoding a target gene sequence / encoding an operational sequence and sterile water; using the primer mixture as a template, performing the first... PCR The reaction yielded the primary spliced ​​product; a second reaction was then performed using start and end primers. PCR The reaction yielded the crude gene synthesis product; nested primers were then used for a third reaction. PCR The reaction yields a nested structure. PCR Products; nested PCR The product was subjected to agarose gel electrophoresis, and the target band was excised and recovered. This application improves upon traditional gene synthesis methods by adding operation sequences to both ends of the synthesized gene sequence and utilizing highly specific nested primers for nested gene synthesis. PCR It can synthesize more than 2000bp in one go. DNA Double-stranded sequences significantly improve the yield and quality of gene synthesis products.

1. Name of the product in this design: Graphical user interface for displaying gene synthesis operation function information of electronic device. 2. Purpose of this design: An electronic device. 3. The key design feature of this product is its graphical user interface. 4. The image or photograph that best illustrates the design's key features: the front view. 5. Purpose of the graphical user interface: The interface of this product is a graphical user interface for displaying operation information for manual liquid flushing and reagent bottle replacement in a biological gene short chain synthesizer. 6. Human-computer interaction method of graphical user interface: The main view is a graphical user interface that displays information on the manual liquid flow function of the synthesizer; clicking the corresponding icon button in the main view can enter the corresponding interface. In the interface, ×××× represents text and / or numbers and / or letters and / or symbols.

The invention relates to a gene synthesis device and a gene synthesis method. The gene synthesis device comprises a controller, a bearing assembly, a first air pressure adjusting assembly, an upper cover assembly and a second air pressure adjusting assembly. The bearing assembly is provided with a first bearing cavity and used for bearing a composite plate. The first air pressure adjusting assembly is electrically connected with the controller, the first air pressure adjusting assembly communicates with the first bearing cavity, and the controller is used for controlling the first air pressure adjusting assembly to adjust the air pressure in the first bearing cavity; the upper cover assembly is provided with a second bearing cavity; the upper cover assembly is arranged on the side, away from the first bearing cavity, of the composite plate. The first bearing cavity and the second bearing cavity are separated through a composite plate; the second air pressure adjusting assembly is electrically connected with the controller and communicates with the second bearing cavity, and the controller is used for controlling the second air pressure adjusting assembly to adjust the air pressure in the second bearing cavity. When the gene synthesis device and method are used for synthesizing the gene, the synthesis cost is low, and the deviation of the synthesis result is small.

The present invention relates to methods of synthesising nucleic acids, in particular to methods of extending an overlapping primer oligonucleotide to generate an oligonucleotide, polynucleotide, gene fragment or gene comprising repeating sequences. The present invention also relates to overlapping primer oligonucleotides for use in nucleic acid synthesis reactions.

The present invention discloses a recombinant plasmid, a construction method and a prokaryotic expression method of μ-conotoxin peptide. A construction method of a recombinant plasmid for prokaryotic expression of μ-conotoxin peptide comprises the following steps: 1) modifying the amino acid sequence of μ-conotoxin peptide; 2) codon optimizing the modified amino acid sequence to obtain an optimized base sequence; 3) performing gene synthesis according to the base sequence and cloning it into a vector to obtain a synthetic plasmid; 4) transforming the synthetic plasmid through Escherichia coli to obtain a transformed plasmid; 5) amplifying the transformed plasmid through a first primer, a second primer and a third primer to obtain an amplified target product; 6) homologously recombine the linearized synthetic plasmid and the amplified target product to obtain a homologous recombination product; transforming the homologous recombination product into Escherichia coli to obtain a recombinant plasmid. The recombinant plasmid of the present invention is conducive to the prokaryotic expression of μ-conotoxin peptide.

The invention relates to a bemisia tabaci gene BtCOX8 and application thereof, and belongs to the field of biological control. The gene BtCOX8 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtCOX8 is a potential efficient RNAi lethal target. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa and orius minutus which are natural enemies of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a good application prospect.

This invention relates to plant genetic engineering, and more particularly to a variegated Phalaenopsis orchid and its preparation method, the preparation method comprising the following steps: (1) according to the target gene with the base sequence shown in SEQ ID No. 1 PePDS (1) Synthesize specific nucleotide fragments; (2) Construct specific nucleotide fragments into a plant virus vector; (3) Introduce the recombinant plant virus vector obtained in step (2) into Phalaenopsis orchid plant material; (4) Screen to obtain Phalaenopsis orchids with silenced target genes. This invention achieves rapid, efficient, and non-transgenic leaf color variegation creation in commercial Phalaenopsis orchid varieties for the first time through VIGS technology; it not only breaks through the technical bottleneck of difficult and long cycle of genetic manipulation of orchid plants, significantly shortening the trait verification cycle, but also induces diverse and highly ornamental variegated leaf phenotypes such as albino, yellow, or purple in different varieties, and has the advantages of simple operation, low cost, and industrialization feasibility.

The invention discloses an expression vector of cyclodextrin glucosyltransferase and construction and application of bacillus subtilis, belongs to the technical field of enzyme engineering, and is characterized in that the amino acid sequence of the cyclodextrin glucosyltransferase is shown as SEQ ID NO. 1; the nucleotide sequence for coding the amino acid sequence of the cyclodextrin glucosyltransferase is as shown in SEQ ID NO. 2; the construction method of the expression vector of the cyclodextrin glucosyltransferase comprises the following steps: carrying out whole gene synthesis on an amino acid sequence as shown in SEQ ID NO.1 to obtain a target gene, and connecting the target gene with a vector plasmid pWB980-ori to obtain an expression vector pWB980-ori-CGT; the method has the beneficial effects that the expression vector pWB980-orii-CGT is obtained, the bacillus subtilis is constructed, the cyclodextrin glucosyltransferase can be stably and efficiently expressed, the bacillus subtilis can be directly treated by full-fermentation reaction enzyme liquid without separation, and the constructed genetically engineered bacterium is shortened in fermentation period and has high enzyme activity and high conversion rate.

The invention discloses a teneplase mRNA (messenger Ribonucleic Acid) medicine and application thereof. The active ingredient of the medicine provided by the invention is mRNA (messenger ribonucleic acid) as shown in SEQ ID NO.5. The invention also protects a recombinant plasmid of the teneplase as shown in SEQ ID NO. 4. Specific DNA molecules are obtained through a series of design and sequence optimization, specific recombinant plasmids are synthesized through gene synthesis, the specific recombinant plasmids are subjected to in-vitro transcription, cell supernatant protein is obtained through transfection of mRNA of different amounts at different time points after transfection, and the cell supernatant protein is used for preparing the recombinant protein. According to the present invention, it is detected through ELISA that the tinipase mRNA can be effectively expressed in different cells of different species, such that the pioneering significance is provided for the development of the tinipase mRNA drug.

The invention relates to the technical field of recombinant protein, in particular to self-assembled calcium chelate keratin RK35DE and a preparation method thereof. According to the invention, the self-assembled calcium-chelated keratin RK35DE is obtained through protein design, whole-gene synthesis, vector construction and escherichia coli expression. The keratin RK35DE is subjected to self-assembly research, and the TEM image of the keratin RK35DE shows that the keratin RK35DE has self-assembly capability. In a calcium chelating ability experiment, compared with that before recombination, the self-assembled calcium-chelated keratin RK35DE disclosed by the invention has a remarkable chelating effect on calcium ions. In the prior art, keratin with self-assembly capability lacks effective coordination capability of forming stable chelates with calcium ions in hydroxyapatite, so that stable compounds are difficult to form and exert effects. The self-assembled calcium chelate keratin researched and developed by the scheme provides a new thought for researching a new hydroxyapatite tooth restoration material, and has an ideal popularization and application prospect.

This invention discloses a method for constructing a multi-target amplicon abundance standard for microorganisms and its application, belonging to the fields of molecular biology detection and microbiome analysis. This standard material screens sequences of common human gut microbiota strains, designs and adds universal primers, and obtains 14 DNA fragments of different lengths through gene synthesis, cloning, and purification, which are then mixed according to a preset abundance. It retains natural characteristics such as primer binding sites and GC content, and can systematically correct technical deviations in amplicon sequencing, solving the problem of species abundance distortion. This standard material functions as both a non-homologous internal reference and a homologous external reference, and can be used for laboratory proficiency testing, reagent kit performance evaluation, and cross-platform data calibration, promoting the leap from relative qualitative to absolute quantitative research in microbiome studies and providing metrological support for the standardization and precision of detection results.