Method for producing diosgenin through microbial fermentation of peltate yam rhizome-yellow ginger

Abstract

The invention discloses a method for producing diosgenin through microbial conversion of peltate yam rhizome-yellow ginger. Yellow ginger dry powder and tap water are mixed according to a massic volume ratio of 1:5-12, urea which is 4.0% of mass of the yellow ginger dry powder, yeast extract which is 1.0% of mass of the yellow ginger dry powder and inorganic salt which is 0.2% of volume of the tap water are supplemented to prepare conversion slurry, the conversion slurry is introduced in bacillus bacterial strain XBT 2011 seed liquid which is 5% of the volume of the tap water, under the condition that the culture temperature is 40 DEG C, the pH is controlled to be about 6.0, the stirring speed is 300-400rpm and the ventilation condition is 2.5-4.0l / min, the conversion is 56-72hrs, conversion slurry is filtered and dried to obtain hydrolysate, the hydrolysate is extracted for 12-16hrs by using 120# gasoline, the volume of the 120# gasoline is one time of that of the hydrolysate, and diosgenin end products are obtained through repeated crystallization. A preservation number of the bacterial strain XBT 2011 is CGMCCNo.6301. The dry weight of the hydrolysate is 28-33% of that of the added yellow ginger dry powder, total saponins of the peltate yam rhizome is approximately converted into diosgenin completely, handling capacity is big, no sugar contains, the pH is close to neutral, and environmental pollution is reduced.

Description

technical field

[0001] The invention relates to a method for preparing diosgenin, in particular to a method for producing diosgenin from dioscia scutellaria-turmeric through microbial transformation. It belongs to the technical field of biological extraction. Background technique

[0002] The most commonly used method to extract diosgenin (saponin) from turmeric is acid hydrolysis. This earliest production process has incomplete hydrolysis, low yield (yield is generally only about 1.7%), huge amount of waste water and serious environmental pollution and many other issues. At present, various enterprises adopt the method of coupling the improved natural fermentation and acid hydrolysis. Natural fermentation, through the enzymatic hydrolysis of turmeric's own endogenous enzymes, converts a part of furostyl alcohol saponin into spiroginyl saponin, which is more easily converted into diosgenin. Therefore, this coupling method produces saponin, and its yield can be increased ...