Soybean conversion method

A technology for soybeans and soybean plants, applied in biochemical equipment and methods, botany equipment and methods, angiosperms/flowering plants, etc., can solve the problems of difficult emergence and low transformation efficiency, and achieve simple and convenient Agrobacterium infection process , high conversion efficiency and increased efficiency
CN103525864AActive Publication Date: 2014-01-22BEIJING DABEINONG BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
Publication Date
2014-01-22

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Abstract

The invention relates to a soybean conversion method which comprises the following steps: cotyledon and the first euphylla of germinated soybean seeds are removed, and naked meristem is obtained; the naked meristem is inoculated to a pretreatment culture medium containing cytokinin for pretreatment; meristem blocks after the pretreatment are infected by agrobacteria. According to the soybean conversion method, the soybean meristem blocks after the pretreatment are used as explant materials for the first time; the explant materials are subjected to high-concentration cytokinin treatment before agrobacterium infection so that the meristem of soybeans can be in an active state and exogenous genes can be inserted easily; meanwhile, the meristem is multiplied and enlarged, so that the infection process is more convenient to operate; in the process of agrobacterium infection, ultrasonic treatment is added, so that the efficiency of insertion of the exogenous genes in a plant genome is greatly improved, and the conversion rate of the soybeans can reach 10%; meanwhile, the conversion period is short, the number of chimera is small, and conversion cost is low.
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Description

technical field

[0001] The invention relates to a method for plant transformation, in particular to a method for transforming soybean tissue. Background technique

[0002] Soybean (Glycine max) originated in China and is the main source of plant protein. It is also an important oil crop and high-protein grain and feed crop in China. The planting area is second only to rice, corn and wheat, ranking fourth. In order to improve soybean herbicide tolerance, resistance to pests and diseases, and physiological adversity, improve soybean quality, and increase soybean yield, in addition to traditional breeding methods, genetic engineering improvement can be achieved through genetic engineering.

[0003] The genetic transformation methods of soybean mainly include Agrobacterium-mediated method, gene gun bombardment method, electric shock method, PEG method, microinjection method, ultrasonic-assisted method, and pollen tube introduction method, among which Agrobacterium-mediated metho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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