31 results about "Zeatin" patented technology

The invention belongs to the technical field of plant tissue culture, and particularly relates to an induction culture medium for embryogenic calluses of rubber tree variety thermal reclamation 628, a method for inducing somatic embryogenesis by using the induction culture medium and application of the induction culture medium. According to the induction culture medium, an improved MS culture medium is used as a basic culture medium, and the induction culture medium further comprises 0.1 g / L to 1 g / L of proline, 0.1 g / L to 1 g / L of arginine, 0.1 g / L to 1 g / L of glutamine, 10 mg / L to 70 mg / L of adenine sulfate, 1 mg / L to 10 mg / L of silver nitrate, 0.2 mg / L to 2 mg / L of 2, 4-D, 0.2 mg / L to 2 mg / L of Zetin and 0.2 mg / L to 2 mg / L of Picloram. According to the method, the somatic embryo occurrence rate can be remarkably increased by optimizing the induction culture medium of the embryogenic callus of the heat reclamation 628, the anther somatic embryo occurrence rate of the heat reclamation 628 of the rubber tree variety can be increased to 50% or above by utilizing the induction culture medium, and the cotyledon embryo occurrence rate can be increased to 20% or above.

The invention discloses a novel ginseng stem cell culture system which comprises a basic culture medium and a growth regulation composition, and the basic culture medium comprises ammonium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate and calcium chloride; the growth regulating composition comprises 2, 4-dichlorphenoxyacetic acid, zeatin, salicylic acid and natural polysaccharide. The basic culture medium also comprises casein hydrolysate, coconut water extract and cane sugar. The composition also comprises an anti-oxidation composition. The invention further discloses a disposable flexible bioreactor for ginseng stem cell culture, the bioreactor comprises a tank body made of a flexible material, an air distribution device, a sterile sampling device and an online parameter monitoring device, and the top of the tank body structure is a cylindrical body and is provided with an inoculation opening, a culture medium liquid supplementing opening, an air outlet and a sampling opening; the bottom is an inverted cone provided with a parameter monitoring device interface and an air inlet, and the air inlet is connected with a ventilation device and an air flow adjusting device. The invention also discloses application of the culture system and the bioreactor.

The invention provides a tissue culture and rapid propagation method for dendrobium nobile, and belongs to the technical field of plant tissue culture. Comprising the following steps: taking a middle stem section of a pretreated new lateral bud as an explant, and sequentially inoculating into a bud induction culture medium for induction culture, a bud proliferation culture medium for proliferation culture and a rooting and seedling strengthening culture medium for rooting culture, the bud induction culture medium comprises the following components: plant hormones, potato powder, zeatin and activated carbon; the bud proliferation culture medium is prepared from the following components: plant hormones, potato powder, mashed apples and trehalose. The rooting and seedling strengthening culture medium comprises the following components: plant hormones, potato powder, mashed bananas and a yeast extract. According to the method disclosed by the invention, the 'Hooke summer end' is taken as a tissue culture object, the rooting rate can be obviously improved by adjusting the components of the culture medium in different stages, improving the budding rate of the explant and increasing the bud proliferation multiple, and the elongation of roots and the growth of plants are facilitated.

The invention discloses a low-molecular-weight buffalo milk polypeptide debitterizing method, and belongs to the technical field of polypeptide processing treatment.The method comprises the steps that S1, buffalo milk is subjected to enzymolysis treatment, and enzymatic hydrolysate is prepared; s2, adding a debitterizing agent into the enzymatic hydrolysate obtained in the S1, and uniformly stirring to obtain a debitterizing solution; s3, sieving and spray drying are performed to prepare the low-molecular-weight buffalo milk polypeptide; wherein the debitterizing agent accounts for 70 to 90 weight percent of the enzymatic hydrolysate; the debitterizing agent is prepared from zein and euglena polysaccharide; the euglena polysaccharide accounts for 3.5 to 15 weight percent of the debitterizing agent; the spray drying conditions are as follows: the air inlet temperature is 175-195 DEG C, the air outlet temperature is 75-90 DEG C, the feeding speed is 10-20 mL / min, and the pressure is 0.8-1.5 MPa. The debitterizing agent is mixed with the enzymatic hydrolysate to achieve a debitterizing effect, and zein can be connected with hydrophobic amino groups generated after enzymolysis, so that the bitterness of polypeptide is greatly reduced; the used euglena polysaccharide can be used as a skeleton to improve the coating of the zein on the polypeptide, so that the debitterizing effect is improved; the added sodium alginate can improve the stability of the polypeptide and has a certain slow-release effect.

The application discloses a production process of trans-zeatin, which comprises the following steps: refluxing E-2-(4-hydroxy-3-methyl-2-butene)-phthalimide with sodium hydroxide in methanol to obtain (E)-4-amino-1-hydroxy-2-methyl-2-butene; filtering and collecting the filtrate and concentrating the filtrate; and reacting the concentrated filtrate with 6-chloropurine in n-propanol and triethylamine as an acid-binding agent to finally obtain trans-zeatin. The application uses E-2-(4-hydroxy-3-methyl-2-butene)-phthalimide as a raw material, and high-purity trans-zeatin is obtained through two steps. The preparation method can effectively improve the yield of trans-zeatin, is simple and convenient to operate, is convenient for industrial popularization, and has good economic and social benefits.

The application discloses high-selenium Drabbea japonica and a culture method thereof. The culture method comprises the following steps: culturing Drabbea japonica seedlings, applying a selenium-containing nutrient solution or a selenium-containing fertilizer after 15 days, and then regularly supplementally applying the selenium-containing nutrient solution or the selenium-containing fertilizer until harvesting; after the first application of the selenium-containing nutrient solution or the selenium-containing fertilizer, a selenium absorption promoter is sprayed every 7-10 days until harvesting. The selenium absorption promoter is a suitable amount of zeatin, abscisic acid or spermidine. In the cultivation process, by adding a suitable compound selenium and strictly limiting the concentration of selenium, high-selenium Drabbea japonica plants can be obtained, and the edible value of the Drabbea japonica plants can be greatly improved. In addition, by adding a suitable amount of zeatin, abscisic acid or spermidine in the cultivation process, the selenium content in the roots of the Drabbea japonica can be greatly improved, and the nutritional value and medicinal value of the Drabbea japonica are greatly improved.

The application discloses a bacillus thuringiensis and application thereof. The bacillus thuringiensis ZF505 provided by the application has a preservation number of CGMCC No.24585 in the China General Microbiological Culture Collection Center. A potting living body test shows that the bacillus thuringiensis can prevent and treat cucumber downy mildew with a highest effect of 73.01%, and the bacillus thuringiensis CGMCC No.24585 can promote the increase of the content of three endogenous hormones of cucumber, wherein gibberellin is increased by 15.61%, indole acetic acid is increased by 5.05%, and zeatin is increased by 5.12%.

The invention discloses a formula of a broad-spectrum culture medium adapted to sterile sowing of multiple species of Chinese orchid, and relates to the technical field of plant tissue culture, the broad-spectrum culture medium adopts a 1 / 2MS basic culture medium, the dosage of a macroelement mother solution is halved, and the dosage of ferric salt, microelement and vitamin mother solution is kept unchanged; 10 mg of modified graphene, 1.5 mg to 2.5 mg of 6-benayl aminopurine (6-BA), 0.3 mg to 0.8 mg of naphthylacetic acid (NAA), 0.2 mg to 0.8 mg of zeatin (ZT), 0.05 mg to 0.2 mg of indolebutyric acid (IBA), 1.2 g to 2.2 g of lactoalbumin hydrolysate, 0.6 g to 1.0 g of activated carbon, 22 g to 32 g of saccharose and 5.5 g to 7.5 g of agar are added into every 1 L of the basic culture medium. The pH value of the culture medium is adjusted to 5.2-5.8. By adding the modified graphene into the culture medium, germination of Chinese orchid seeds is effectively promoted. Meanwhile, a complete nutrition system and a root system induction mechanism enhance the growth vigor of the seedlings and improve the survival rate of the seedlings, and meanwhile the universality of the culture medium is remarkably improved through the formula of the culture medium.

The invention provides an efficient rapid propagation method for blueberry mycorrhizal seedlings, and relates to the technical field of blueberry mycorrhizal seedling propagation. The efficient rapid propagation method of the blueberry mycorrhizal seedlings comprises the following specific steps: S1, selection of explants, S2, disinfection of the explants, S3, bud induction of the explants, S4, subculture multiplication culture, and S5, rooting of tissue culture seedlings outside a bottle and inoculation of blueberry mycorrhizal fungi. In the disinfection process, when the explant is soaked in 70% ethyl alcohol for disinfection for 3 min and soaked in 0.1% mercury bichloride for disinfection for 7 min, the pollution rate of the Lackxi explant is low, the survival rate is high, in the explant bud induction process, when the concentration of zeatin hormone (ZT) is 1.5 mg / L, the bud induction rate, the proliferation multiple and the bud growth vigor reach the best, and the survival rate is high. In the subculture multiplication culture process, when the concentration of zeatin hormone (ZT) is 0.6 mg / L, the Lackxi plant has many clumpy buds, and the plant is high and robust and grows fast.

The application provides an induction method and an induction medium for inducing adventitious buds of leaf petioles of Asparagus cochinchinensis, and solves the technical problem of low efficiency of inducing germination of adventitious buds of rhizomes or aerial stems in the prior art. The induction method of the adventitious buds of the leaf petioles of the Asparagus cochinchinensis comprises the following steps: sterilizing and disinfecting leaf petiole explants and transplanting and transferring the leaf petiole explants to an induction medium of the adventitious buds, wherein the induction medium of the adventitious buds comprises 6-benzylaminopurine, forchlorfenuron, zeatin, 6-furfurylaminopurine, indole-3-butyric acid, a multi-vitamin tablet, a coagulant, sucrose and a nutrient medium. The method uses the leaf petiole of the Asparagus cochinchinensis as the explant, can efficiently induce the adventitious buds, establishes a plant regeneration technical system of the Asparagus cochinchinensis, realizes rapid propagation, has high regeneration efficiency, and has abundant sources of the explants. Meanwhile, the method establishes a mature, stable and efficient regeneration system of the Asparagus cochinchinensis, and has important significance for transgenic breeding of the Asparagus cochinchinensis.

The application discloses application of a tobacco zeatin glucosyltransferase gene in regulating tobacco seed germination time. The gene is NtZOG, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the amino acid sequence of which is shown as SEQ ID NO. 2. Research finds that by knocking out the tobacco zeatin glucosyltransferase gene NtZOG from wild tobacco K326, a tobacco mutant with the NtZOG gene knocked out is constructed. By comparing the germination of the wild tobacco K326 and the mutant, it is found that the germination rate of the mutant is significantly lower than that of the wild tobacco at 4.5 days of development, and is as low as about 50%, so it can be seen that the tobacco zeatin glucosyltransferase gene NtZOG has an important regulating effect on tobacco seed germination, and the expression of the gene can be regulated to regulate the germination time of tobacco, and the application has a potential application prospect in the tobacco planting field.

This invention provides a method for inducing callus and seedling formation using *Amorphophallus konjac* leaves as explants, and its application. The method for inducing callus using *Amorphophallus konjac* leaves as explants includes: inoculating *Amorphophallus konjac* leaves into a callus induction medium and culturing to obtain callus; the callus induction medium includes: 1 / 2 MS basal medium, naphthaleneacetic acid, 6-benzylaminopurine, thidiazuron, zeatin, sucrose, and agar. This invention uses *Amorphophallus konjac* leaves as explants for callus induction, which is convenient to obtain, and *Amorphophallus konjac* leaves are abundant, resulting in rapid propagation and high efficiency, greatly improving the quality and induction rate of callus.

The application provides a use of a banana tissue endogenous hormone zeatin in resisting banana wilt, and belongs to the technical field of plant protection. The banana tissue endogenous hormone is zeatin, and experiments prove that in vitro tests (tissue culture bottles and greenhouses) of the zeatin or lutein show that the zeatin can effectively inhibit the growth of TR4 mycelium, exhibits good anti-banana wilt fungus activity, has a significant prevention and control effect on the banana wilt fungus (TR4), and does not affect the growth of seedlings. The application provides a new idea and method for preventing and controlling TR4, and lays a foundation for in-depth exploration of the prevention and control of the banana wilt disease TR4.

The invention discloses a method for inducing virus-free sweet potato matrix culture, which relates to the technical field of plant propagation. The method comprises the following steps: S1, selecting virus-free test tube seedlings of 8-10 cm, training the seedlings in a plant growth room until the growth state is stable to obtain virus-free seedlings, and transferring the virus-free seedlings to a matrix culture device; S2, using zeatin ribosyl monophosphate (ZR) as a solute and 1 / 2 Hoagland nutrient solution as a solvent to prepare a nutrient solution with a ZR concentration of 0.8-16 mg / L, and adding the prepared nutrient solution to the matrix culture device; S3, adding 1 / 2 Hoagland nutrient solution every 40 days after transplanting the seedlings, and cultivating the culture room temperature at 24±2°C, a photoperiod of 14 / 10 h, and a light intensity of 80-100 µmol•m‑2•s‑1. The present invention provides a matrix cultivation method for virus-free seed potatoes, which cultivates virus-free seed potatoes and supplies them to growers instead of the traditional method of supplying virus-free potato seedlings. The growers can use the virus-free seed potatoes to cultivate virus-free seedlings in the production base according to their needs, so as to better meet the market demand for sweet potato production.

The application belongs to the technical field of biological genetic engineering, and particularly relates to application of a ZmDIR4 gene in regulation of drought sensitivity of corn. The application finds through experiments that after the ZmDIR4 gene is knocked out, the dry matter accumulation of mutant corn plants after drought stress, the activity of antioxidant enzymes in the antioxidant metabolic pathway, the accumulation of proline, chlorophyll and lignan, and the activation degree of the hormone metabolic pathway (zeatin and abscisic acid) are all significantly higher than those of wild type and overexpression materials, proving that the ZmDIR4 gene has a negative regulation function in drought stress resistance of corn. The ZmDIR4 gene can be used to create new corn germplasm with improved drought resistance, and provides a new idea for corn drought resistance.

The invention belongs to the technical field of plant growth regulators, and particularly relates to a medicament and a method for promoting litchi flowering. The invention provides a medicament for promoting litchi to flower. The medicament is prepared from ethephon, paclobutrazol, zeatin, trans-zeatin, 1-aminocyclopropane carboxylic acid, indole-3-methyl acetate and N6-isopentene adenine. The zeatin, the trans-zeatin, the 1-aminocyclopropanecarboxylic acid, the indole-3-methyl acetate and the N6-isopenteneadenine in the insecticide are all beneficial to formation and differentiation of litchi flower buds, and the ethephon and the paclobutrazol have a plant shoot control effect. Results of the embodiment show that the agent can improve the white point emergence rate and the flowering rate of the litchis, and the problem that the yield of the litchis exceeds the year is fundamentally solved. The agent provided by the invention can improve the flowering rate of litchi trees, is safer and more efficient, and can save the labor cost at the same time.

The invention discloses a quinoa pre-harvest sprouting resistance molecular marker system and application, and the system screens out key genes and key metabolites significantly related to pre-harvest sprouting resistance in a quinoa pustulation period through conjoint analysis of transcriptome and metabolome. The key gene relates to synthesis, metabolism and signal transduction pathways of eight hormones, namely ABA, GA, IAA, JA, ETH, BR, CTK and SA; the key metabolites comprise IAA, L-tryptophan, zeatin, SA and the like. The molecular marker system can be used for carrying out resistance identification and screening on chenopodium quinoa germplasm resources by detecting the expression level or content of the key genes and metabolites, and is applied to molecular marker-assisted breeding and genetic engineering breeding, so that a new preharvest sprouting-resistant chenopodium quinoa variety is rapidly and accurately cultivated, and the application prospect is wide. And the pre-harvest sprouting problem in quinoa production is effectively solved.

The present invention discloses a method for inducing callus of Clivia leaves, which includes: culturing sterile Clivia leaves in a hormone medium to obtain callus of Clivia leaves; the hormone medium includes an MS medium with a pH value of 5.8 and a hormone solution; the hormone solution is indoleacetic acid and zeatin with a volume ratio of 2:1, or naphthaleneacetic acid and zeatin with a volume ratio of 2:1, or naphthaleneacetic acid and 6-benzylaminopurine with a volume ratio of 1:1, or naphthaleneacetic acid and zeatin with a volume ratio of 1:6; wherein, the concentrations of indoleacetic acid, zeatin, naphthaleneacetic acid, and 6-benzylaminopurine are all 1 g / L. The method for inducing callus of Clivia leaves in the present invention provides suitable conditions for inducing callus of Clivia leaves by preparing a suitable medium, and can quickly induce the generation of callus on Clivia leaves.

The invention relates to the technical field of crop planting, and particularly provides a method for improving the salt tolerance of rice in a seedling stage by using trans-zeatin. The trans-zeatin (CAS: 1637-39-4) provided by the invention can effectively improve the salt tolerance of rice in a seedling stage only through simple spraying under a specific concentration, and has important agricultural significance for planting crops in a saline-alkaline environment.

This invention relates to a recombinant photosynthetic bacterial strain with high tocopherol production, its construction method, and its applications. The recombinant strain knocks out the photosynthetic gene repressor transcription factor PpsR and integrates the mevalonate MEV pathway gene from *Paragonimococcus zeatans*, the p-hydroxyphenylpyruvate dioxygenase (HPPD) gene from *Pseudomonas putida*, the urokinase chlorophyllase (HPT) gene from *Synostemma pentaphyllum*, the tocopherol cyclase (TC) gene from *Arabidopsis thaliana*, and feedback-resistant 3-deoxy-D-arabinohepenoyl-7-phosphate synthase (AroG*). D146N Genes and prephenyl acid dehydrogenase TyrA* M53I Genes. This recombinant photosynthetic bacterium strain achieves highly efficient heterologous synthesis of tocopherol by knocking out regulatory genes and integrating multiple metabolic modules, with a fermentation yield of up to 3.51 g / L, showing broad prospects for industrial application.

The invention relates to a photosynthetic bacterium recombinant strain with high yield of tocopherol as well as a construction method and application of the photosynthetic bacterium recombinant strain. The photosynthetic bacterium recombinant strain knocks out a photosynthetic gene inhibition transcription factor PpsR of photosynthetic bacteria; a mevalonic acid MEV pathway gene for producing paracoccus zeatin, a p-hydroxyphenylpyruvate dioxidase HPPD gene from pseudomonas putida, a uromelanic acid chlorophyllin transferase HPT gene from synechocystis, a tocopherol cyclase TC gene from arabidopsis thaliana, a recombinant plasmid and a recombinant plasmid are integrated; the invention also discloses a 3-deoxy-D-arabinoheptulose-7-phosphate synthase AroG * D146N gene resistant to feedback and a pre-benzoate dehydrogenase TyrA * M53I gene resistant to feedback. According to the photosynthetic bacterium recombinant strain, efficient heterologous synthesis of tocopherol is achieved by knocking out regulation genes and integrating multi-way metabolism modules, the fermentation yield can reach 3.51 g / L, and the photosynthetic bacterium recombinant strain has wide industrial application prospects.

This invention discloses a wearable electrochemical sensor, its preparation method, and its applications. The wearable electrochemical sensor uses an ultrathin flexible stainless steel sheet modified with conductive carbon adhesive as the working electrode, combined with a counter electrode and a reference electrode to form a three-electrode system. It is used to detect plant hormones in microregions of plant tissue, achieving simultaneous detection of three plant hormones—indole-3-acetic acid, salicylic acid, and zeatin—in plant tissue using differential pulse voltammetry. This sensor exhibits good electrode stability during the detection of plant hormones in microregions of plant tissue, has a large detection range, and the detection method is convenient and simple. In addition to detecting plant hormones in microregions of plant tissue, it also enables in-situ detection of plants.

The invention discloses bacillus velezensis CMF18 and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251028. The strain disclosed by the invention has phosphorus dissolving capacity, potassium dissolving capacity and medium element magnesium dissolving capacity. The strain can generate xylanase and has the function of promoting organic matter decomposition. The strain of the invention has the ability to synthesize and secrete plant growth promoting substances, including auxin hormone gibberellin (GA3) and cytokinin plant hormone zeatin (Z). And the microbial fertilizer has excellent comprehensive capability and can be used for preparing microbial fertilizer strains for promoting the growth of crops.

The invention belongs to the technical field of litchi breeding, and particularly relates to a litchi seedling breeding method. According to the method, IBA, KT and the litchi pulp extract are combined, so that the explants are favorably induced to germinate; 6-BA, IBA and natrolite powder are added into the second culture medium, on the basis of reducing tissue culture browning, germination differentiation and development of litchi stems are promoted, and high-vitality plantlets can be obtained; 2, 4-D, zeatin, mannitol and activated carbon are added for rooting culture, so that the rooting rate of tender shoots (light green seedlings) is increased, and the survival rate of subsequent litchi seedlings is increased. Therefore, the technical scheme provided by the invention can be applied to breeding of litchi seedlings, and is simple, convenient and rapid, and the survival rate is high.

The present application belongs to the field of woody plant callus culture and differentiation, and particularly relates to a culture medium combination and method for inducing the germination of woody plant callus. The present application provides a culture medium combination and method for inducing the germination of woody plant callus. In the combined culture medium, NAA, 2,4-D, 6-BA, gibberellin (GA3) GA3, glutamine, extract of Scutellaria baicalensis Georgi, hydrolyzed milk protein, zeatin, and sucrose are conducive to the induction of callus formation and differentiation into buds, and effectively prevent callus browning. The results of examples show that the culture medium combination of the present application can induce the germination of woody plant callus in a short time, reduce the browning rate, and the total flavonoid content of the obtained seedlings is high.

The invention provides application of cytokinin glycosyltransferase, and belongs to the technical field of genetic engineering. According to the invention, a novel gene (the nucleotide sequence is as shown in SEQ ID NO.3) in highland barley is found, and the protein expressed by the gene (the amino acid sequence is as shown in SEQ ID NO.4) can convert trans-zeatin into trans-zeatin 3-O-glucoside, so that the stress resistance level of the highland barley is improved. According to the invention, the gene segment is subjected to in-vitro expression to obtain cytokinin glycosyl transferase, and in an in-vitro reaction, glucose is successfully used as a glycosyl donor and trans-zeatin is successfully used as a receptor to prepare the trans-zeatin 3-O-glucoside. The gene is also transferred into tobacco, so that cytokinin glycosyltransferase is also expressed in a tobacco plant, transzeatin 3-O-glucoside is further generated, and the value of transzeatin 3-O-glucoside is improved. The novel gene, the recombinant vector, the recombinant bacteria, the protein and the transgenic plant provided by the invention have good application prospects.

The invention provides an induction method and application of tea tree leaf calluses. A method for inducing calluses of tea tree leaves comprises the following steps: selecting mature leaves of a tea tree, reserving petiole parts, cleaning for later use without disinfection, inoculating an obtained explant to an induction culture medium for induction culture, the induction culture medium being selected from any one of an MS culture medium, 2.5 mg / L of 6-BA and 0.1 mg / L of NAA; the MS culture medium is prepared from 1.5 mg / L of 6-BA, 0.5 mg / L of zeatin and 0.1 mg / L of IBA; the MS culture medium is prepared from 2.0 mg / L of zeatin and 0.1 mg / L of IBA. According to the method, it is found for the first time that cane sugar is a key cause of infectious microbe breeding in tea tree tissue culture, by removing cane sugar in the culture medium, the culture medium can be kept sterile for a long time even in an open environment (no bacteria grow for five months), the industrial problem of tea tree endophyte and infectious microbe pollution is successfully solved, and the pollution rate is almost zero.

The invention belongs to the technical field of plant molecular biology and crop genetic breeding, and discloses DNA (deoxyribonucleic acid) methylated transferase ItCMT2 related to sweet potato tuberous root development as well as an encoding gene and application of the DNA methylated transferase ItCMT2. According to the invention, the ItCMT2 gene is cloned and identified from a sweet potato diploid related species Ipomoeatrifidavar.Y22, and the ItCMT2 gene is used for encoding a protein containing BAH and C5MTase structural domains. By constructing an ItCMT2 gene knock-down (KD) transgenic sweet potato model and combining agronomic character identification, methylation analysis, hormone quantification and omics conjoint analysis, it is proved that ItCMT2 can maintain sweet potato genome methylation, the root weight of a single sweet potato plant can be reduced by 51.3%-75.7% when expression of ItCMT2 is reduced, and a unique hormone spectrum of zeatin, auxin and jasmonic acid is presented; meanwhile, the starch is reduced and the starch grain shape is abnormal along with the increase of root tuber lignin.

The present invention discloses a method for culturing nitrogen-fixing Anabaena, which comprises the following steps: preparing a culture medium and culturing under light; sterilizing leucine and arginine at high temperature separately and adding them to the sterilized basal culture medium; sterilizing zeatin by membrane filtration and adding it to the sterilized basal culture medium; inoculating nitrogen-fixing Anabaena seed liquid; culturing under light and shaking for 7 to 9 days, thereby obtaining a microalgae culture solution having an algae cell density of 3.12×10 7 ~5.35×10 7 The present invention can make nitrogen-fixing Anabaena grow rapidly, increase the number of algal cells, shorten the culture time, and reduce the production cost. The nitrogen-fixing Anabaena culture solution can be used as a microbial fertilizer.

The present application provides a method for blueberry seedling cultivation and its application. The method includes: selection and treatment of plants and explants, induction culture of axillary buds in an improved culture medium, and zeatin (ZT) decreasing subculture culture, and further cultivation of subculture seedlings into commercial seedlings. The method provided in the present application uses a basic culture medium prepared from improved MS and WPM culture media, so that the explants germinate quickly, the subculture cycle is short, the seedlings grow well, the branches are reasonable, and the buds are thick. The induced budding rate of blueberries is increased to 92.5%, and the proliferation rate after five subcultures reaches 404.8 times. The one-step seedling cultivation method used in commercial seedling cultivation has a rooting rate of 98.2% after 25 days of cultivation, shortens the seedling cultivation time by an average of 19.4 days, and the root system is dispersed and uniform. This solution effectively improves seedling cultivation efficiency, seedling quality and plant growth after planting and reduces the risk of variation. It fundamentally solves various root system problems that seriously affect survival, growth and yield after planting.