18 results about "Zeatin" patented technology

The invention discloses a low-molecular-weight buffalo milk polypeptide debitterizing method, and belongs to the technical field of polypeptide processing treatment.The method comprises the steps that S1, buffalo milk is subjected to enzymolysis treatment, and enzymatic hydrolysate is prepared; s2, adding a debitterizing agent into the enzymatic hydrolysate obtained in the S1, and uniformly stirring to obtain a debitterizing solution; s3, sieving and spray drying are performed to prepare the low-molecular-weight buffalo milk polypeptide; wherein the debitterizing agent accounts for 70 to 90 weight percent of the enzymatic hydrolysate; the debitterizing agent is prepared from zein and euglena polysaccharide; the euglena polysaccharide accounts for 3.5 to 15 weight percent of the debitterizing agent; the spray drying conditions are as follows: the air inlet temperature is 175-195 DEG C, the air outlet temperature is 75-90 DEG C, the feeding speed is 10-20 mL / min, and the pressure is 0.8-1.5 MPa. The debitterizing agent is mixed with the enzymatic hydrolysate to achieve a debitterizing effect, and zein can be connected with hydrophobic amino groups generated after enzymolysis, so that the bitterness of polypeptide is greatly reduced; the used euglena polysaccharide can be used as a skeleton to improve the coating of the zein on the polypeptide, so that the debitterizing effect is improved; the added sodium alginate can improve the stability of the polypeptide and has a certain slow-release effect.

The application discloses a production process of trans-zeatin, which comprises the following steps: refluxing E-2-(4-hydroxy-3-methyl-2-butene)-phthalimide with sodium hydroxide in methanol to obtain (E)-4-amino-1-hydroxy-2-methyl-2-butene; filtering and collecting the filtrate and concentrating the filtrate; and reacting the concentrated filtrate with 6-chloropurine in n-propanol and triethylamine as an acid-binding agent to finally obtain trans-zeatin. The application uses E-2-(4-hydroxy-3-methyl-2-butene)-phthalimide as a raw material, and high-purity trans-zeatin is obtained through two steps. The preparation method can effectively improve the yield of trans-zeatin, is simple and convenient to operate, is convenient for industrial popularization, and has good economic and social benefits.

The application discloses high-selenium Drabbea japonica and a culture method thereof. The culture method comprises the following steps: culturing Drabbea japonica seedlings, applying a selenium-containing nutrient solution or a selenium-containing fertilizer after 15 days, and then regularly supplementally applying the selenium-containing nutrient solution or the selenium-containing fertilizer until harvesting; after the first application of the selenium-containing nutrient solution or the selenium-containing fertilizer, a selenium absorption promoter is sprayed every 7-10 days until harvesting. The selenium absorption promoter is a suitable amount of zeatin, abscisic acid or spermidine. In the cultivation process, by adding a suitable compound selenium and strictly limiting the concentration of selenium, high-selenium Drabbea japonica plants can be obtained, and the edible value of the Drabbea japonica plants can be greatly improved. In addition, by adding a suitable amount of zeatin, abscisic acid or spermidine in the cultivation process, the selenium content in the roots of the Drabbea japonica can be greatly improved, and the nutritional value and medicinal value of the Drabbea japonica are greatly improved.

The application discloses a bacillus thuringiensis and application thereof. The bacillus thuringiensis ZF505 provided by the application has a preservation number of CGMCC No.24585 in the China General Microbiological Culture Collection Center. A potting living body test shows that the bacillus thuringiensis can prevent and treat cucumber downy mildew with a highest effect of 73.01%, and the bacillus thuringiensis CGMCC No.24585 can promote the increase of the content of three endogenous hormones of cucumber, wherein gibberellin is increased by 15.61%, indole acetic acid is increased by 5.05%, and zeatin is increased by 5.12%.

The invention provides an efficient rapid propagation method for blueberry mycorrhizal seedlings, and relates to the technical field of blueberry mycorrhizal seedling propagation. The efficient rapid propagation method of the blueberry mycorrhizal seedlings comprises the following specific steps: S1, selection of explants, S2, disinfection of the explants, S3, bud induction of the explants, S4, subculture multiplication culture, and S5, rooting of tissue culture seedlings outside a bottle and inoculation of blueberry mycorrhizal fungi. In the disinfection process, when the explant is soaked in 70% ethyl alcohol for disinfection for 3 min and soaked in 0.1% mercury bichloride for disinfection for 7 min, the pollution rate of the Lackxi explant is low, the survival rate is high, in the explant bud induction process, when the concentration of zeatin hormone (ZT) is 1.5 mg / L, the bud induction rate, the proliferation multiple and the bud growth vigor reach the best, and the survival rate is high. In the subculture multiplication culture process, when the concentration of zeatin hormone (ZT) is 0.6 mg / L, the Lackxi plant has many clumpy buds, and the plant is high and robust and grows fast.

The application provides an induction method and an induction medium for inducing adventitious buds of leaf petioles of Asparagus cochinchinensis, and solves the technical problem of low efficiency of inducing germination of adventitious buds of rhizomes or aerial stems in the prior art. The induction method of the adventitious buds of the leaf petioles of the Asparagus cochinchinensis comprises the following steps: sterilizing and disinfecting leaf petiole explants and transplanting and transferring the leaf petiole explants to an induction medium of the adventitious buds, wherein the induction medium of the adventitious buds comprises 6-benzylaminopurine, forchlorfenuron, zeatin, 6-furfurylaminopurine, indole-3-butyric acid, a multi-vitamin tablet, a coagulant, sucrose and a nutrient medium. The method uses the leaf petiole of the Asparagus cochinchinensis as the explant, can efficiently induce the adventitious buds, establishes a plant regeneration technical system of the Asparagus cochinchinensis, realizes rapid propagation, has high regeneration efficiency, and has abundant sources of the explants. Meanwhile, the method establishes a mature, stable and efficient regeneration system of the Asparagus cochinchinensis, and has important significance for transgenic breeding of the Asparagus cochinchinensis.

The application discloses application of a tobacco zeatin glucosyltransferase gene in regulating tobacco seed germination time. The gene is NtZOG, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the amino acid sequence of which is shown as SEQ ID NO. 2. Research finds that by knocking out the tobacco zeatin glucosyltransferase gene NtZOG from wild tobacco K326, a tobacco mutant with the NtZOG gene knocked out is constructed. By comparing the germination of the wild tobacco K326 and the mutant, it is found that the germination rate of the mutant is significantly lower than that of the wild tobacco at 4.5 days of development, and is as low as about 50%, so it can be seen that the tobacco zeatin glucosyltransferase gene NtZOG has an important regulating effect on tobacco seed germination, and the expression of the gene can be regulated to regulate the germination time of tobacco, and the application has a potential application prospect in the tobacco planting field.

This invention provides a method for inducing callus and seedling formation using *Amorphophallus konjac* leaves as explants, and its application. The method for inducing callus using *Amorphophallus konjac* leaves as explants includes: inoculating *Amorphophallus konjac* leaves into a callus induction medium and culturing to obtain callus; the callus induction medium includes: 1 / 2 MS basal medium, naphthaleneacetic acid, 6-benzylaminopurine, thidiazuron, zeatin, sucrose, and agar. This invention uses *Amorphophallus konjac* leaves as explants for callus induction, which is convenient to obtain, and *Amorphophallus konjac* leaves are abundant, resulting in rapid propagation and high efficiency, greatly improving the quality and induction rate of callus.

The application provides a use of a banana tissue endogenous hormone zeatin in resisting banana wilt, and belongs to the technical field of plant protection. The banana tissue endogenous hormone is zeatin, and experiments prove that in vitro tests (tissue culture bottles and greenhouses) of the zeatin or lutein show that the zeatin can effectively inhibit the growth of TR4 mycelium, exhibits good anti-banana wilt fungus activity, has a significant prevention and control effect on the banana wilt fungus (TR4), and does not affect the growth of seedlings. The application provides a new idea and method for preventing and controlling TR4, and lays a foundation for in-depth exploration of the prevention and control of the banana wilt disease TR4.

The invention discloses a quinoa pre-harvest sprouting resistance molecular marker system and application, and the system screens out key genes and key metabolites significantly related to pre-harvest sprouting resistance in a quinoa pustulation period through conjoint analysis of transcriptome and metabolome. The key gene relates to synthesis, metabolism and signal transduction pathways of eight hormones, namely ABA, GA, IAA, JA, ETH, BR, CTK and SA; the key metabolites comprise IAA, L-tryptophan, zeatin, SA and the like. The molecular marker system can be used for carrying out resistance identification and screening on chenopodium quinoa germplasm resources by detecting the expression level or content of the key genes and metabolites, and is applied to molecular marker-assisted breeding and genetic engineering breeding, so that a new preharvest sprouting-resistant chenopodium quinoa variety is rapidly and accurately cultivated, and the application prospect is wide. And the pre-harvest sprouting problem in quinoa production is effectively solved.

This invention relates to a recombinant photosynthetic bacterial strain with high tocopherol production, its construction method, and its applications. The recombinant strain knocks out the photosynthetic gene repressor transcription factor PpsR and integrates the mevalonate MEV pathway gene from *Paragonimococcus zeatans*, the p-hydroxyphenylpyruvate dioxygenase (HPPD) gene from *Pseudomonas putida*, the urokinase chlorophyllase (HPT) gene from *Synostemma pentaphyllum*, the tocopherol cyclase (TC) gene from *Arabidopsis thaliana*, and feedback-resistant 3-deoxy-D-arabinohepenoyl-7-phosphate synthase (AroG*). D146N Genes and prephenyl acid dehydrogenase TyrA* M53I Genes. This recombinant photosynthetic bacterium strain achieves highly efficient heterologous synthesis of tocopherol by knocking out regulatory genes and integrating multiple metabolic modules, with a fermentation yield of up to 3.51 g / L, showing broad prospects for industrial application.

The invention relates to a photosynthetic bacterium recombinant strain with high yield of tocopherol as well as a construction method and application of the photosynthetic bacterium recombinant strain. The photosynthetic bacterium recombinant strain knocks out a photosynthetic gene inhibition transcription factor PpsR of photosynthetic bacteria; a mevalonic acid MEV pathway gene for producing paracoccus zeatin, a p-hydroxyphenylpyruvate dioxidase HPPD gene from pseudomonas putida, a uromelanic acid chlorophyllin transferase HPT gene from synechocystis, a tocopherol cyclase TC gene from arabidopsis thaliana, a recombinant plasmid and a recombinant plasmid are integrated; the invention also discloses a 3-deoxy-D-arabinoheptulose-7-phosphate synthase AroG * D146N gene resistant to feedback and a pre-benzoate dehydrogenase TyrA * M53I gene resistant to feedback. According to the photosynthetic bacterium recombinant strain, efficient heterologous synthesis of tocopherol is achieved by knocking out regulation genes and integrating multi-way metabolism modules, the fermentation yield can reach 3.51 g / L, and the photosynthetic bacterium recombinant strain has wide industrial application prospects.

This invention discloses a wearable electrochemical sensor, its preparation method, and its applications. The wearable electrochemical sensor uses an ultrathin flexible stainless steel sheet modified with conductive carbon adhesive as the working electrode, combined with a counter electrode and a reference electrode to form a three-electrode system. It is used to detect plant hormones in microregions of plant tissue, achieving simultaneous detection of three plant hormones—indole-3-acetic acid, salicylic acid, and zeatin—in plant tissue using differential pulse voltammetry. This sensor exhibits good electrode stability during the detection of plant hormones in microregions of plant tissue, has a large detection range, and the detection method is convenient and simple. In addition to detecting plant hormones in microregions of plant tissue, it also enables in-situ detection of plants.

The present application belongs to the field of woody plant callus culture and differentiation, and particularly relates to a culture medium combination and method for inducing the germination of woody plant callus. The present application provides a culture medium combination and method for inducing the germination of woody plant callus. In the combined culture medium, NAA, 2,4-D, 6-BA, gibberellin (GA3) GA3, glutamine, extract of Scutellaria baicalensis Georgi, hydrolyzed milk protein, zeatin, and sucrose are conducive to the induction of callus formation and differentiation into buds, and effectively prevent callus browning. The results of examples show that the culture medium combination of the present application can induce the germination of woody plant callus in a short time, reduce the browning rate, and the total flavonoid content of the obtained seedlings is high.

The invention provides application of cytokinin glycosyltransferase, and belongs to the technical field of genetic engineering. According to the invention, a novel gene (the nucleotide sequence is as shown in SEQ ID NO.3) in highland barley is found, and the protein expressed by the gene (the amino acid sequence is as shown in SEQ ID NO.4) can convert trans-zeatin into trans-zeatin 3-O-glucoside, so that the stress resistance level of the highland barley is improved. According to the invention, the gene segment is subjected to in-vitro expression to obtain cytokinin glycosyl transferase, and in an in-vitro reaction, glucose is successfully used as a glycosyl donor and trans-zeatin is successfully used as a receptor to prepare the trans-zeatin 3-O-glucoside. The gene is also transferred into tobacco, so that cytokinin glycosyltransferase is also expressed in a tobacco plant, transzeatin 3-O-glucoside is further generated, and the value of transzeatin 3-O-glucoside is improved. The novel gene, the recombinant vector, the recombinant bacteria, the protein and the transgenic plant provided by the invention have good application prospects.

The invention provides an induction method and application of tea tree leaf calluses. A method for inducing calluses of tea tree leaves comprises the following steps: selecting mature leaves of a tea tree, reserving petiole parts, cleaning for later use without disinfection, inoculating an obtained explant to an induction culture medium for induction culture, the induction culture medium being selected from any one of an MS culture medium, 2.5 mg / L of 6-BA and 0.1 mg / L of NAA; the MS culture medium is prepared from 1.5 mg / L of 6-BA, 0.5 mg / L of zeatin and 0.1 mg / L of IBA; the MS culture medium is prepared from 2.0 mg / L of zeatin and 0.1 mg / L of IBA. According to the method, it is found for the first time that cane sugar is a key cause of infectious microbe breeding in tea tree tissue culture, by removing cane sugar in the culture medium, the culture medium can be kept sterile for a long time even in an open environment (no bacteria grow for five months), the industrial problem of tea tree endophyte and infectious microbe pollution is successfully solved, and the pollution rate is almost zero.

The invention belongs to the technical field of plant molecular biology and crop genetic breeding, and discloses DNA (deoxyribonucleic acid) methylated transferase ItCMT2 related to sweet potato tuberous root development as well as an encoding gene and application of the DNA methylated transferase ItCMT2. According to the invention, the ItCMT2 gene is cloned and identified from a sweet potato diploid related species Ipomoeatrifidavar.Y22, and the ItCMT2 gene is used for encoding a protein containing BAH and C5MTase structural domains. By constructing an ItCMT2 gene knock-down (KD) transgenic sweet potato model and combining agronomic character identification, methylation analysis, hormone quantification and omics conjoint analysis, it is proved that ItCMT2 can maintain sweet potato genome methylation, the root weight of a single sweet potato plant can be reduced by 51.3%-75.7% when expression of ItCMT2 is reduced, and a unique hormone spectrum of zeatin, auxin and jasmonic acid is presented; meanwhile, the starch is reduced and the starch grain shape is abnormal along with the increase of root tuber lignin.

This invention discloses a rapid propagation method for *Nitraria tangutorum* seedlings induced from leaves, belonging to the field of tissue culture. The method includes: rooting culture of explant leaves, with a rooting medium of 1 / 2 MS + 0.5 mg / L indolebutyric acid; proliferation culture, with a proliferation medium of MS + 2.0 mg / L 6-benzylaminopurine + 1.0 mg / L zeatin + 2.0 mg / L indolebutyric acid; and rooting culture, with a rooting medium of 1 / 3 MS + 2.0 mg / L indolebutyric acid. This invention achieves rapid propagation of *Nitraria tangutorum* under artificially controlled conditions using tissue culture (from explant culture to seedling rooting takes only 35-45 days). Compared with previous tissue culture methods, this invention eliminates the callus culture step, avoiding browning, vitrification, and contamination that occur in callus culture, thus significantly accelerating the seedling formation rate and greatly improving seedling quality.