160 results about "Callus formation" patented technology

The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.

The invention relates to a novel technology for raising hard-rooting fruit tree cutting seedlings, in particular to a jujube tree hardwood cutting seedling raising method. The method mainly comprises the following steps: cutting acquisition and shearing, cutting sand storage, field selection and land preparation, plastic house establishment, high ridge bedding, cutting treatment and cutting, ground film covering, seedling management strengthening and finished product seedling obtaining. By adopting the plastic house, the covering ground film for temperature rise and hormone treatment, the invention has the advantages that the cutting callus formation and rooting are promoted, the survival rate is high, the seedlings can be obtained in the year of cutting, the labor and the time are saved, the period is short, the cost is low, the effect is taken quickly, the yield is high, the jujube tree seedling raising technology is advanced and the rapid development of Chinese jujube trees is certainly promoted.

The invention discloses a method for quick rooting through cuttage of chrysanthemums. Specifically, the method includes the following steps of 1 preparing a cuttage bed, 2 preparing cuttings, 3 carrying out cuttage and 4 carrying out seedling growing and management, wherein in the step 1, matrixes sprayed with sterilizing agents are placed in a plurality of seedling growing hole trays; in the step 2, the cuttings are cleared up, the lower portions of the cuttings are cut and sequentially placed in the sterilizing agents and rooting agent solutions, and the rooting agent solutions are a mixed solution composed of indolebutyric acid, a multiwalled carbon nanotube and water; in the step 3, the center of each hole of each seedling growing hole tray is vertically provided with a hole in an inserted mode with a bamboo stick, and then the processed cuttings are vertically cut in the holes and watered; in the step 4, all-day shading treatment is conducted on chrysanthemum seedlings at the callus forming period of seedling stages, shading treatment only needs to be carried out when sunlight is intensive at the callus complete forming period of the seedling stages, and the sterilizing agents need to be sprayed onto the chrysanthemum seedlings at the root system forming period of the seedling stages. The method has the advantages that the cuttings can be promoted to quickly root, rooting periods are short, orderly rooting is achieved and rooting quality is good.

The invention provides a taxus chinensis propagating method. The method is characterized by comprising the following steps that 1, taxus chinensis branches are selected; 2, bactericide is used for treating the branches; 3, a plant growth regulator is used for treating the branches; 4, water culture is adopted for inducing the branches to take root; 5, a nutrient solution is added for curing. By the adoption of the method for propagating taxus chinensis, restriction of cultivated land resources can be gotten rid of, and the propagation area is increased; meanwhile, the influence on the survival rate from external environment is reduced to the maximum, time for callus forming and rooting is shortened, the stem cutting survival rate is remarkably increased, the restriction that due to poor environmental factors such as temperature, humidity, soil and water quality, the stem cutting survival rate is influenced by an original propagation means is broken through, the current situation that taxus chinensis genetic resources are scanty can be relieved, and great economic value can be created.

The invention discloses a method for rapidly breeding fritillaria pallidiflora by using a tissue culture technique. The method mainly comprises the steps of selecting and disinfecting explants, treating materials, inducing callus, carrying out subculture, carrying out rooting culture, seedling hardening, transplanting and the like, wherein the formation rate of the callus of the fritillaria pallidiflora is 93%, the proliferation rate is 6 times, the rooting percentage is 92%, the final survival rate is 90%, and the quality and the output of the fritillaria pallidiflora are both greatly improved; and moreover, the method simulates the growing environment of the fritillaria pallidiflora, the temperature is low in the culture process, chernozem is mainly taken as the culture medium, and the quality is greatly improved as the fritillaria pallidiflora grows in such a simulated wild environment.

Osteosynthesis constructs for treating bone fractures and bone screws for use in such systems are disclosed. The bone screws include a threaded front section configured for engagement with cortical bone, a threaded mid-section and an unthreaded neck section configured for limited movement within the near cortex of the bone. Osteosynthesis constructs of the type disclosed promote secondary healing by allowing for substantially parallel motion at the near and far cortex within the effective motion range for callus formation.

A method is disclosed for producing a transgenic cotton plant comprising the steps of (a) obtaining cottonfibrous root explants, (b) culturing the fibrous root explants to induce callus formation, (c) exposing root callus to a culture of Agrobacterium tumefaciens that harbors a vector comprising an exogenous gene and a selectable marker, the Agrobacterium being capable of effecting the stable transfer of the exogenous gene and selection agent resistance gene to the genome of the cells of the explant, (d) culturing the callus in the presence of the selection agent to which the selection agent resistance gene confers resistance so as to select for transformed cells, (e) inducing somatic embryo formation in the selected callus culture, and (f) regenerating the induced somatic embryos into whole transgenic cotton plants.

The invention relates to a pawpaw cutting processing method. The method includes the following specific steps that 1, a cutting is selected; 2, the tail end of the cutting is peeled and incised and the cutting is soaked in an auxin solution; 3, the cutting is inserted into a nutrient solution with the cooperation of electric field treatment and magnetic field treatment; 4, after callus tissue is formed, the cutting can be cut into soil. Through experiments, the cutting rooting rate is increased by means of the pawpaw cutting processing method, meanwhile, after the cutting grows up to a seedling, the disease resistance of the seedling is obviously improved, the incidence rate of various diseases is greatly reduced, and the method is worthy of application and popularization.

The invention belongs to the technical field of plant biology, in particular relates to a method of tissue culture of Medicago sativa L. By way of developing a novel tissue culture technical method, the invention focuses on improvement and innovation of two technical nodes (seed disinfection and callus formation and differentiation) in culture steps. With adoption of a mercury bichloride and hydrogen peroxide compound disinfection method, the invention realizes the purposes of thorough disinfection effect and low pollution rate and minimum damage to seeds. Finally, the invention solves the problem of severe pollution to current explants. Meanwhile, a method of TDZ and 6-BA is adopted, thereby, the callus induction rate and the formation rate are remarkably increased, the callus quality is improved, the differentiation capacity of the callus is reserved to a maximum extent, the formation of embryoid sprouts is promoted so that the novel technical method of the tissue culture of the Medicago sativa L. which is efficient, saves time, and has low toxicity and low browning, is obtained.

The invention discloses a gardenia multi-branch floating bed water planting cuttage method. The gardenia multi-branch floating bed water planting cuttage method is characterized by comprising the following steps that a large branch with 3-5 shoots is selected, the diameter of the root of the branch is larger than 0.5cm, terminal buds grow on the two sections of the shoots, and 3-5 leaves are left for each shoot; the root part, with the length ranging from 2cm to 5cm, of the branch is immersed into the mixed solution of alpha-naphthylacetic acid and indolebutyric acid to be dipped for 2-3 seconds; a sunshade net shed with the 50% sunshade ratio is established on the outdoor cleaning water surface, and cutting slips are inserted into plastic foam which serves as a cuttage support to be cultured and protected. The gardenia multi-branch floating bed water planting cuttage method has the advantages that the water surface floating bed cuttage is adopted, the oxygen in the water is sufficient, the forming of the callus is facilitated, the water temperature is stable, the temperature difference between day and night is small, the air humidity on the water surface is high, the branch absorbs water easily, the leaves cannot wither and fall even when encountering water loss and can carry out the photosynthesis to synthesize nutrient substances, the root system is well developed, the ability of absorbing nutrients is strong, the survival rate in the process of cultivation is high, and the growing speed is high; frequent spraying and water changing are omitted, managing links such as intertillage and weeding in a nursery garden land and workloads are removed, the gardenia multi-branch floating bed water planting cuttage method is simple and easy to implement, and mass propagation can be achieved.

The invention belongs to the field of plant tissue culture of plant cell engineering techniques, and in particular relates to a tissue culture propagation method for reducing the tissue culture browning rate of traxacum koksaghyz. The tissue culture propagation method comprises the following steps: inoculating, namely, selecting mature seeds of a traxacum koksaghyz strain; performing induced differentiation on calluses; inducing callus formation; proliferating adventitious buds; performing rooting culture; separating multiple shoots into single plants, and transferring to a rooting culture medium for rooting culture; performing acclimatization and transplantation, thereby obtaining regenerated plants of traxacum koksaghyz. By adopting the tissue culture propagation method, a method for effectively reducing the tissue culture browning rate of traxacum koksaghyz and rapidly propagating traxacum koksaghyz is provided, the tissue culture browning rate of traxacum koksaghyz is greatly reduced through technical measures such as adjusting components of culture mediums used in different stages and adding an antioxidant, the propagation coefficient of traxacum koksaghyz is improved, the browning rate can be reduced to be less than 35% when the culture mediums provided by the invention is adopted, and the browning rate can be further reduced to be less than 10% when Na2S2O3 is added.

The invention discloses a peach tree green stock-green branch cut grafting rapid seedling growing method. A half-lignification green branch is used as a scion to be grafted to a green stock of a nutrition bag in a cut grafting mode, leaves below the grafted port of the green stock are kept, the grafted port and the scion are bound by plastic strips in a sealed mode, only bud eyes are exposed, as the lignification degree of the green stock and the scion is low, tissue cells are active, the leaves left on the green stock can continue to conduct photosynthesis to manufacture nutrient substances which are supplied to calluses, coalescence of the grafted port is achieved rapidly in a growing period, and therefore the rate of survival of grafting of the lignification scion in a resting period is improved by 20%-30% compared with annual rootstock seedlings . Grafted seedlings can be conveyed out of a flower nursery for garden building from August to September, the seedlings of the nutrition bag do not need pruning, leaf cutting and watering, the root caps are not damaged after the seedlings are planted, a seedling buffering period is avoided after planting, and the leaves fall down and dormancy occurs after the seedlings sprout and slightly mature. The rate of survival of planting of garden building is high, the seedlings grow rapidly, fructification is achieved early, the yield is high, and the peach tree green stock-green branch cut grafting rapid seedling growing method is particularly suitable for garden building of mountainous regions where are dry and have no irrigation conditions in winter and spring.

The present invention discloses a blueberry softwood cutting seedling method. The method comprises the following steps: selecting and trimming cutting woods, soaking the trimmed woods with a rooting agent containing boron and IBA for 8-10 hours, and inserting the woods into a matrix, wherein the humidity of the matrix is kept at 80% or above, and the temperature is controlled at 20-28 DEG C. The method of the present invention is simple to study, high in operability, good in rooting effect and high in utility value, wherein the rooting rate reaches up to 91.18%, so that the callus formation rate and the rooting number can be significantly improved.

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising twoAP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence ofexogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The invention discloses a method of fast breeding peony, comprising: a) getting axillary bud bulk tip or tender leaf stalk from plants and culturing them in the culture medium until the callus formation and germination, b) cutting off the clump bud from the callus and culturing them until the bud grows to a height of 3-5 cm; c) inserting the bud in step b) into solutions containing indolebutyric acid, then culturing in the MS solid culture medium added with indolebutyric acid, benzyl amino adenine, active carbon, saccharose and macronutrient whose concentration reduced halves to form small plants; d) replanting the small plants. The invention develops a method of mass fast breeding peony by employing technique of plant cell tissue and forms a mass production process which produces tube sprout of peony ten thousand plants per year.

The invention discloses a mist cutting cultivation method using blueleaf honeysuckle shoots. The method mainly includes: setting a seedbed and supporting facilities, taking and treating cuttings, performing cuttage in May to July, managing the seedbed and the like, and specially includes: before calluses of the cuttings form, intermittently spraying mist to the cuttings for multiple times each day during seedbed management; after the calluses of the cuttings form, spraying the mist for less times; and maintaining cuttage substrate moist, and controlling the temperature of the seedbed at 18-26 DEG C till seedlings emerge after two to three months. By the method, the survival rate of the shoots subject to cuttage is high, cultivation cycle is short, and cultivation cost is low. Important technical basis is provided for large-area popularization of blueleaf honeysuckle in Shandong even in north China by the method.

The invention relates to a method for obtaining the monoploid plant of Zantedeschia aethiopica by anther culture, belonging to the technical field of flower culture. The method comprises the steps that the young bud of Zantedeschia aethiopica is taken out in the bud stage for sterilization and the anther is taken out and placed on the callus inducement culture medium for culturing and inducing the microspores into callus; the callus is put on the bud inducement culture medium for culturing and inducing the callus to form bud; the bud is put on the radication culture medium for culturing and inducing the bud to radicate and the monoploid plant of Zantedeschia aethiopica is obtained. The method is applied to isolated culture of anther of Zantedeschia aethiopica; on average, 2.4 percent of the anther can induce the microspores to form the callus and each callus can differentiate 6.8 buds and the rate of monoploid plant is 69.6 percent. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.

The invention relates to a method for gardenia tissue culture, which comprises the following steps: (a), gardenia seeds are used as explants so as to carry out callus induction; (b), the callus is split in liquid differential medium so as to obtain callus with buds; (c), the callus with buds is cultivated in bud multiplication solid medium so as to obtain more regeneration buds; (d), all the buds are cut off from base parts and transferred to rooting medium for root induction. According to the invention, the gardenia seeds are used as the explants, the callus is induced to form on MS culture medium with different hormone combination; a method of combining solid culture and liquid culture is adopted to induce the differentiation of buds and roots, so as to obtain gardenia regeneration seedlings. In the invention, a tissue culture system taking gardenia reproductive organs as the explants is built, and an experimental platform for further obtaining high effective-component content of gardenia through cell culture and the research of gardenia transgenosis is built.

The invention belongs to the technical field of traditional Chinese medicine tissue culture breeding seedling growing, and discloses a shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema. The shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema comprises following steps: material selection; culture medium preparation; tender shoot disinfection; tender shoot inoculation; culture management; and hardening seedling and transplanting. The shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema is simple, and isconvenient in operation; the rooting rate is 94%; the survival rate after transplanting is 96%; seedling growing cost is reduced greatly; tender shoots are taken as a tissue culture material, so thatphotosynthesis can be realized, and dependence on nutrients in the culture medium is reduced; after harvesting, the tender shoots are immersed in a low temperature tranexamic acid solution, so that tender shoot cell division is accelerated, and tender shoot rooting rate is increased; mecobalamin and sodium selenite are added into a MS culture medium, so that growth of competitive bacteria is inhibited, the activity of protoplasm of cells at wounds is increased, callus formation is accelerated, and seedling growing efficiency is increased.

A method is disclosed for producing a transgenic cotton plant by Agrobacterium-mediated transformation of petiole tissue. The method comprises the steps of (a) obtaining cotton petiole explants, (b) exposing the petiole explants to a culture of Agrobacterium tumefaciens that harbors a vector comprising an exogenous gene and a selectable marker, the Agrobacterium being capable of effecting the stable transfer of the exogenous gene and selection agent resistance gene to the genome of the cells of the petiole explant, (c) culturing the petiole explants to induce callus formation, (d) selecting transformed callus that expresses the exogenous gene, (e) culturing the selected callus in suspension culture to induce formation of embryoids, (f) regenerating the embryoids into whole transgenic cotton plants.

The invention belongs to the technical field of agricultural planting, and particularly relates to a plant grafting repair agent. The plant grafting repair agent is prepared from the following raw materials in parts by weight: 6-10 parts of indoleacetic acid, 6-13 parts of 10% coconut juice, 7-13 parts of 0.5% yeast extract and 3-7 parts of honey, 15-20 parts of willow extract, 15-20 parts of a disease-resistant bactericide and 5-9 parts of a nutrition agent. The plant grafting repair agent comprises the disease-resistant bactericide, a growth-promoting agent, the nutrition agent and the like,wherein the disease-resistant bactericide can prevent a grafting joint from breeding bacterium and infection; the nutrition agent can improve the healing efficiency, and the honey used can not only inhibit the breeding of bacteria, but also enhance the moisture degree of the grafting joint to prevent chapping, and simultaneously can lock moisture by acting together with super absorbent resin, sothat the water resistance is effectively improved; the indoleacetic acid, the coconut juice, the yeast extract and the willow extract can promote callus formation and accelerate the healing of grafting joint on the one hand, and willow extract contains more rooting elements, so that the survival rate of grafting can be improved.