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Blueberry softwood cutting seedling method

A green branch cutting and blueberry technology, which is applied in the field of blueberry green branch cutting and seedling cultivation, can solve the problems of unsatisfactory rooting effect, failure to meet the market demand for large-scale blueberry propagation, low rooting rate, etc., and achieve callus formation rate and The number of roots is increased, the technology is simple and easy to learn, and the rooting effect is good

Inactive Publication Date: 2016-02-03
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In terms of production, the rapid breeding of blueberries mainly adopts the method of tissue culture propagation, and a small part adopts blueberry hard branch and green branch cutting technology, but mainly focuses on the separate use of various exogenous hormones (IAA, IBA, etc.) Promote the rooting of blueberry twig cuttings, but the rooting rate is low, and the rooting effect is not ideal. These technologies can not meet the market demand for blueberry large-scale propagation.

Method used

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  • Blueberry softwood cutting seedling method
  • Blueberry softwood cutting seedling method
  • Blueberry softwood cutting seedling method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The experiment was carried out in the greenhouse of the blueberry demonstration base in Gongcun, Baima Town, Meishan City, Sichuan Province, and the green branches of two varieties of Nangaobush blueberry, Lanfeng and Hebert, were selected from the blueberry planting base in Gongcun, Baima Town, Meishan City, Sichuan Province.

[0022] From March to April 2013, select southern highbush blueberry Herbert and Lanfeng's healthy green branches of the year and bring them back to the laboratory. The cuttings should be cut from trees that grow vigorously and are free of diseases and insect pests (it is better to choose hard, mature branches If there is a virus disease in the orchard, the cutting tree should be more than 15cm away from the diseased tree. Cutting branches are the best For one-year-old vegetative branches, the middle and lower parts of the branches should be selected for cutting as much as possible, and the branches should be trimmed into 7-10cm cuttings with 4-5 ...

Embodiment 2

[0062] Example 2 Effects of different self-made rooting agents on the rooting rate and rooting efficiency of southern highbush blueberry at different rooting stages

[0063] Table 6 and Table 7 show the change of rooting rate of southern highbush blueberries Herbert and Lanfeng at different rooting stages. It can be seen from Table 6 that when the Herbert variety was cut for 23 days, the 200mg·L -1 The rooting rate of cuttings treated with boron compound IBA was 11.67%, which was significantly higher than other treatments (P1 The rooting rate increased by 10.00%, and rooting did not appear in CK2 treatment; at 30 days, 200mg·L -1 The rooting rate of boron compound IBA was still significantly higher than that of other treatments, compared with CK 1 Increased by 26.66%; on the 37th day of cutting, 300mg·L -1 Boron compound IBA has the highest rooting rate (with 200mg·L -1 The difference of boron complex IBA treatment was not significant, respectively 46.67% and 43.33%), compa...

Embodiment 3

[0071] Example 3 Effects of different self-made rooting agents on the final rooting quality of southern highbush blueberry

[0072] Table 8 and Table 9 show the final rooting conditions of blueberry Hebert and Lanfeng cuttings treated with different rooting agents. It can be seen from Table 8 that the rooting statistics of the Herbert variety after 70 days of cuttings (full cycle) show that the rooting rate from high to low is in order: 5% sucrose>100mg L -1 Boron>10% sucrose>200mgL -1 Boron>2% sucrose>300mg·L -1 Boron>CK 1 >50mg·L -1 Vitamin B>200mg·L -1 Vitamin B>100mg·L -1 Vitamin B. Among them, 5% sucrose compound IBA had the highest rooting rate, reaching 91.18%, and its average rooting number also reached the highest, which was 16.40; 100mg·L -1 The rooting rate of boron compound IBA was the second, reaching 86.84%, and the third was treated by 10% sucrose compounding, and the rooting rate reached 86.49%. Cuttings treated with sucrose compounded with a certain co...

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Abstract

The present invention discloses a blueberry softwood cutting seedling method. The method comprises the following steps: selecting and trimming cutting woods, soaking the trimmed woods with a rooting agent containing boron and IBA for 8-10 hours, and inserting the woods into a matrix, wherein the humidity of the matrix is kept at 80% or above, and the temperature is controlled at 20-28 DEG C. The method of the present invention is simple to study, high in operability, good in rooting effect and high in utility value, wherein the rooting rate reaches up to 91.18%, so that the callus formation rate and the rooting number can be significantly improved.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a method for growing blueberry green branch cutting seedlings. Background technique [0002] At present, my country's blueberry planting industry is developing rapidly, the planting area continues to expand, the demand for seedlings is large, and the insufficient supply of seedlings has become a bottleneck hindering the development of my country's blueberry industry. However, because blueberries are shallow-rooted plants with underdeveloped root fibers and no root hairs, the requirements for soil pH, soil moisture, air permeability, drainage, climatic conditions, and management methods are relatively strict, so rooting is extremely difficult. [0003] At present, tissue culture technology is mainly used for rapid propagation of blueberries in China, but some microorganisms are often carried on the surface and inside of explants. At the same time,...

Claims

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Application Information

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IPC IPC(8): A01G1/00
CPCA01G22/00
Inventor 汪志辉郭健熊博余锋廖明安龚荣高汤浩茹张小艾邓群仙吕秀兰廖玲曹淑燕古咸杰李清南叶霜邱霞高婧斐石冬冬张婷婷曾海琼荣毅代琳李立佼
Owner SICHUAN AGRI UNIV
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