149 results about "Root number" patented technology

The invention discloses a rapid propagation method of culturing medical anoectochilus roxburghii tissue through which seedling is formed in only one step. The invention mainly consists of the flowing steps: firstly, culture medium is prepared by three steps of preparation, prepackaging and sterilization; secondly, explant is treated and inoculated, which step has two conditions: when a natural growth plant is used as an explant, the step consists of three steps of cleaning, sterilizing and inoculation, and when a tissue culture sterilized seedling is used as an explant, the sterilized inoculation is directly carried out; thirdly, cultivation; and fourthly, transplantation. The invention uses the middle stem of an anoectochilus roxburghii plant, which is produced by subculture, as an explant. A stem section which contains a stem node is inoculated on culture medium under sterile condition. The results show that after the optimum culture medium is cultured for four months, the average seedling number of each explant is 4.8; the average net increased number of the leaves of each plant is 3.6; the average increased root number is 2.5 and the average net increased fresh weight is 525.6mg. The growing condition of the tissue culture seedling is quite good after being transplanted to substrate. The survival rate of plants achieves to above 95 percent after three months. The invention can greatly shorten the tissue culture seedling time of anoectochilus roxburghii, significantly improve the culture efficiency and reduce the seedling cost. The invention can be used as an important method of producing anoectochilus roxburghii seedlings.

The invention discloses a method for tissue culture method of huperizia serrata. The method comprises the following steps of: selecting an explant; killing surface bacteria; performing inoculation and cultivation; killing endophyte; performing propagation cultivation; and performing rooting cultivation. The huperizia serrata is a precious medicinal pteridophyte which has a tough requirement on a habitat, grows slowly under a natural condition and has a long production period. In a conventional method, propagation coefficient and biomass are low and requirements on medicament production cannot be met. Due to the adoption of the method, the differentiation rate of a huperizia serrata test tube plantlet is up to 90 percent, monthly multiplication multiple is up to 2.1 to 3.5, rooting rate is up to 100 percent, a root system grows quickly, root number is up to 1.6 to 3.4 per plant, a regeneration seedling grows healthily and the root system is developed. The method has the advantages of effectively restraining the pollution and browning of a huperizia serrata explant, promoting the survival and growth of the explant and providing important technical support for the realization of manual mass planting of the huperizia serrata along with simpleness, convenience, practicability, high efficiency and low cost.

The invention discloses a dendrobium officinale sprout rapid propagation method with high efficiency. The method comprises the following steps: a) selecting dendrobium officinale shoot tip or aseptic seedling as explants, performing induction and propagation culture of protocorm like bodies; b) performing differentiation and seeding formation on protocorm like bodies; c) culturing strong plantlet and rootage of seedling grown thickly; and d) hardening seedling and transplanting of the aseptic seedling. According to the invention, shoot tip and aseptic seedling are taken as the explants, thereby the problem that stem bud and top bud as the explants to induce the protocorm like bodies, the explant source is limited can be solved, the mature protocorm like bodies can be rapidly and continuously obtained with large amount, the incidence rate of the protocorm like bodies can reach as high as 99%, the seedling differentiation coefficient can reach as high as 50 strains / 0.1g protocorm like bodies, the period for acquiring the seedling is 4 months, the seedling is tidy, the rooting rate is 100%, the root length is about 5cm, and the root number can reach 10 strips / crowd, the transplanting survival rate can reach as high as more than 95%, and the purposes of shortening the seedling period, increasing the propagation coefficient and unlimited germplasm source can be reached. The rapid propagation method can rapidly produce large batch of sprouts, and is suitable for large scale production.

The invention discloses a random access detection device and a detection method in a TD-LTE (Time Division-Long Term Evolution) system. The device comprises a data extraction unit, a Fourier transform unit, a spectrum shifting unit, a local root sequence generating unit, a relative detection unit and a threshold detection unit, wherein the data extraction unit is used for extracting base band data; the Fourier transform unit is used for carrying out discrete Fourier transform on extracted data to obtain a frequency domain signal; the spectrum shifting unit is used for carrying out spectrum shifting on the frequency domain signal to obtain a frequency domain signal after the spectrum shifting; the local root sequence generating unit is used for generating N1 local frequency domain root sequences according to a logical root number broadcasted in broadcast information; the relative detection unit is used for carrying out relative frequency domain detection on the frequency domain signal after the spectrum shifting and the local frequency domain root sequences to obtain N1 time domain relative values; and the threshold detection unit is used for detecting and judging the time domain relative values according to a set threshold value, judging whether a terminal is allowed to be accessed into the system or not and calculating timing advance. The device and the method can be used for quickly detecting a random access precursor in the TD-LTE system, thereby greatly reducing the computation complexity and improving the operation efficiency.

The invention discloses a cutting cultivation method for acer palmatum, which belongs to the field of horticultural production. The method comprises the following steps: inserting the cutting slips of the acer palmatum into a rooting agent cutting block which is dipped with a para-hydroxybenzoic acid containing 150 mg / l of IBA and having a mass ratio 0.001; and managing the cutting slips of the acer palmatum inserted into the rooting agent cutting block according to a full exposure cutting seedling cultivation mode, wherein calluses are formed after about 45 days; and the cutting slips of the acer palmatum inserted into the rooting agent cutting block can be transplanted after about 70 days. Tests show that the root-forming rate of the acer palmatum reaches 92 percent, the average formed root number of the cutting slips is 3.8 and an average root length is 3.5 cm. The cutting cultivation method for acer palmatum not only can quickly cultivate rare acer palmatum varieties in large scale under the condition of keeping the intrinsic excellent property of the original varieties, but also can provide a reference for plant varieties which are difficult to be cultivated by cutting, thereby having great popularization value.

The invention relates to a method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo, comprising the following steps of: (1) culturing mycorrhizal fungi by utilizing a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP2 deuteromycotina Fusariumsp, mycorrhizal fungi LP3, or mixed strain deuteromycotina Verticilliumsp of the mycorrhizal fungi LP2 deuteromycotina Fusariumsp and the mycorrhizal fungi LP3; (2) culturing tissue culture seedlings of the dendrobium officinale kimura et migo; (3) preparing a nutrient solution; (4) carrying out water culture and training the tissue culture seedlings; and (5) carrying out planting and mycorrhizalculturing of the dendrobium officinale kimura et migo. The method for culturing, planting and mycorrhizal production of the dendrobium officinale kimura et migo, provided by the invention, can promote growth of dendrobium officinale kimura et migo seedlings obviously; and compared with a contrast plant, the dendrobium officinale kimura et migo cultured by inoculating pathogen has increased rooting numbers, high survival rate, longer plant length, better growth vigor, obviously expanded internodes, obviously big stems, improved fresh weight and dry weight and obviously enhanced stress resistance of a pathogen-inoculated plant.

The invention proposes a high-speed target Doppler velocity measurement method based on the least square method, and the main idea of the method is that the method comprises the steps: determining a radar, supposing that there is a high-speed target in a radar detection range, and calculating the fuzziness of the high-speed target in the radar detection range and the blind speed of the high-speedtarget in the radar detection range; calculating a rough measurement value of the radial speed of the high-speed target in the radar detection range according to the fuzziness of the high-speed targetin the radar detection range and the blind speed of the high-speed target in the radar detection range; calculating a blind speed matrix of the high-speed target in the radar detection range according to the rough measurement value of the radial speed of the high-speed target in the radar detection range, and calculating the Doppler fuzziness root number vector of the high-speed target in the radar detection range based on the least square method; and calculating the real radial speed of the high-speed target in the radar detection range based on the Doppler fuzziness root number vector of the high-speed target in the radar detection range and the blind speed matrix of the high-speed target in the radar detection range.

The invention discloses a regeneration gardening seedling nursing medium suitable for plant seedling nursing cultivation. The medium is obtained by fully mixing the components in percentage by volume: 45-50 percent of forestry and agricultural residues, 20-25 percent of rotted animal excrement, 5-10 percent of humic acid, 2-5 percent of rare earth, 3-5 percent of wood vinegar, 2-5 percent of an enzymic preparation and 2-5 percent of an active substance. The invention also discloses a preparation method for the regeneration gardening seedling nursing medium suitable for plant seedling nursing cultivation. The regeneration gardening seedling nursing medium suitable for plant seedling nursing cultivation, which is provided by the invention is large in rooting number and has regeneration property and recycling performance; nutrition can be stabilized, the activity of the medium is improved, the disease resistance and the stress resistance of plants are enhanced, and the survival rate is increased.

The invention discloses a kiwifruit micro-grafting method and a kiwifruit seedling growing method, and belongs to the technical field of plant grafting. Two kiwifruits which are 'Hongyang' and 'Miliang 1' kiwifruits are adopted as grafted objects, an in-vitro rapid propagation system for grafting the two kiwifruits is established, explants used for grafting, an initial culture medium, a subculturemedium, a rooting medium, and the grafting method and a grafting medium are systematically studied to determine the in-vitro rapid propagation system adopting 'Miliang 1' kiwifruit tissue culture seedlings as rootstocks and the 'Hongyang' kiwifruit as cion, so other parameters facilitating the improvement of the induction rate, the tissue proliferating and rooting number and the grafted seedlingsurvival rate are determined, the propagation time is shortened, and the excellent traits of two kiwifruit varieties are reserved; and the control of environmental parameters of grafting greatly improves the survival rate of the grafted seedlings and improves the efficiency of existing kiwifruit production systems.

The present invention provides a whole arc section satellite remote-control Doppler compensation method by calculating satellite orbit and using DDS to realize the compensation, wherein, the satellite orbits calculation method comprises that input data of earth station location coordinates (latitude, longitude and height), satellite transient Keplerian orbit 6 root number including major semi-axis, eccentricity, obliquity, right ascension of the ascending node and mean anomaly, and time point, earth station remote-control time period and time step, and remote-control signal carrier frequency, are used for performing geometry calculation to obtain accurate orbit location of the satellite within the remote-control time period, furthermore, a Doppler frequency bias value, a Doppler frequency first-order change rate and a Doppler frequency second-order change rate are obtained. The calculated Doppler corresponding data are transmitted to the DDS module to generate a frequency with a sign opposite to that of the Doppler frequency bias value and a same absolute value for modulating remote-control signals and generating Doppler frequency bias compensation. According to the present invention, after receiving remote-control signals, the satellite can realize capture of remote-control signal carriers by using a smaller phase-lock loop bandwidth, therefore, carrier capture time is shortened, carrier capture capability is improved, and interference to useful signals from the noise in the loop bandwidth is reduced.

The invention discloses a small elliptic target reentry forecasting method under a sparse data condition. N circles of data collected in the past 5 days are processed, a numerical method is adopted tocarry out track determination on the single circle of data, the Kepler root number is adopted as a root number system, a semi-numerical method is adopted to carry out track integration, and a least square method is adopted to fit a ballistic coefficient of a reentry target. Compared with the prior art, the invention provides a reentry forecasting method combining the numerical method and the semi-numerical method for the characteristics of the sparse data and the small elliptic orbit. The problems that under the condition of sparse data, the multi-circle data joint orbit determination residual error is too large or not convergent, and the ballistic coefficient is difficult to determine through single-circle data orbit determination are effectively solved.

The invention discloses a greenhouse fig cutting propagation seedling nursing method. A fig current-year semi lignification branch with 15cm long work as a cutting slip; the lower end of the cutting slip is disinfected and immersed into ABT root growth prompting agent with concentration of 800mg / L; in an optimized daylight greenhouse, an electric heating wire is paved on the internal bottom of a bed, so temperature difference of 10 to 15 DEG C can be achieved between the bottom and external environment; the well-processed cutting slips are inserted into cutting slip base material mixed by turf, decomposed cow dung, vermiculites, perlites and bone meal; after the cutting, water content of the greenhouse can reach 20 to 25% due to full-light exposure mist spraying management; after cutting, leaf fertilizer is sprayed once to No.45d, No.60d and No.75d cutting slips; and then normal management is conducted. Survival rate of the fig seedling can reach over 95% by the use of the greenhouse fig cutting propagation seedling nursing method; cutting slip callus and adventitious root apparition is shortened; in particular, the method most favors the cutting slip seedling callus; rooting numbers, root length and rooting rate can be improved; seedling stress resistance capability can be enhanced; and fig seedling healthy growth can be prompted.

The invention discloses a soilless straw matrix of rice dry-raising water pipe and a seedling culture method. The method includes the following steps that a seedbed and the soilless straw matrix are manufactured; by means of the deep-ditch and high-box mode, water and fertilizer are controlled and growth of rice seedlings is regulated through a seedling box, wherein the seedling age of seedlings transplanted by machines is prolonged by 5-10 days, and the crop rotation contradiction of rice transplantation is effectively relieved; meanwhile, the soilless straw matrix matched with seedling culture of the method is formed by smashing oilseed rape straw serving as the main raw material and adding disinfectant, a water-retaining agent, modifier, an acid regulating agent, fertilizer and the like; a plastic tray with the specification of 58.8 cm*28 cm*2 cm is used for seedling culture; seedlings grow in the disinfected and appropriate acid condition; growth of seedlings is regulated through the water control and fertilizer control measures in the seedling culture process, and therefore the aims of being strong in seedling, large in root number, thick in rod and green in leaf are achieved. Straw is directly used as the soilless straw matrix after being smashed, the production processes of soil taking, soil screening and the like are reduced, and the labor intensity of rice farmers is lowered.

The invention provides a tissue culture method for an early-maturing pear stem section and a culture medium, and belongs to the technical field of plant cell molecule biology. The tissue culture method comprises the following steps of performing induced differentiation culture on an explant through an initial inoculation culture medium to obtain a primary tissue culture seedling; performing multiplication culture on the primary tissue culture seedling through a multiplication culture medium, then cutting off new branches, transferring into a seedling enhancing culture medium for subculture, and transferring into a rooting culture medium A and a rooting culture medium B for rooting culture. The culture medium for the tissue culture of the early-maturing pear stem section is formed by one or more than two of the initial inoculation culture medium, the multiplication culture medium, the seedling enhancing culture medium and the rooting culture medium. According to the tissue culture method disclosed by the invention, a disinfection method is safe and short in time; the multiplication coefficient, the rooting rate, the average root length and the average root number of the tissue culture seedlings are obviously increased.

The invention provides a method for enhancing the root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet. In the production process, MS solid medium is utilized in the production process as the basic medium, and the Grosvener Siraitia single link stems with buds are taken as explants. The method is characterized in that Angustmycin and spermine are added into the MS solid medium, and regenerated plants with complete roots and seedlings, few callus and developed and robust root system can be obtained in the two production processes of starting cultivating of explants and subculture enrichment culture, which can be transplanted out of bottles and can also be propagated and enlarged continuously. Conventional cultivation needs 25 days, the number of roots of each plant is great than or equal to 8, and the process of generating roots in the traditional method can be completely omitted.

The invention discloses a tissue-culturing and rapid-propagating method of dragon fruits, and in particular relates to the technical field of plant propagation. According to the invention, dragon fruit stems are used as explants; multiplication culture, elongation culture and rooting culture are respectively carried out. A multiplication culture method comprises the following steps of wetting the explants by using 70% alcohol, sterilizing and washing, and putting the washed dragon fruit explants on a 1 / 2 MS culture medium to pre-culture for one week; putting the pre-cultured dragon fruit explants in a culture medium A to culture for 28-32 d so as to obtain dragon fruit sprouts, wherein the culture temperature is controlled within (25+ / -2) DEG C; the illumination time is 12h.d<-1>; and the illumination intensity is 20001x. According to the invention, the dragon fruit sprouts are robust; regeneration seedlings are rapid in growth speed; the rooting number is high; generated roots are robust; the tissue-culturing and rapid-propagating method is a rapid-propagating technology of dragon fruits; raw materials used in the invention are wide in source and low in application cost; and industrialized, scale and intensive production of dragon fruit seedlings can be realized easily.

The present invention discloses a tea tree tissue-cultured seedling directly-rooting method capable of raising survival rate of tea tree tissue-cultured seedlings, simplifying rooting operation program and shortening culture period. Said method includes the following steps: using tea tree tissue-cultured seedling as experimental material, utilizing a hormone with a certain concentration to make treatment, after 60 days, the survival rate of tea tree tissue-cultured seedlings can be up to above 70%, the rooting rate of survival seedling is up to 90%, and the average root number and root length are respectively 9.4 and 3.64cm.

The invention relates to a breeding technology of improved varieties, in particular to a breeding technology of improved varieties of plum and apricot seedlings. The improved varieties of seedlings are cultivated by the following steps: breeding field selection, pre-sowing treatment and germination cultivation, scion grafting, post-grafting treatment and seedling outplanting. The pre-sowing treatment is sand storage lamination. The breeding technology of improved varieties of plum and apricot seedlings provided by the invention has the advantages of disinfecting through pre-sowing treatment, preserving the good and rouging and preventing and controlling diseases and insect pests in germination cultivation and post-scion treatment, and ensuring no virus in seedling production; by raising seedlings in nutrition pockets, the root systems of young seedlings are all in the nutrition pockets, and the whole pockets with soil are transplanted while transplanting, so that the survival rate is improved, and the average survival rate is 96% or more; the main root number of the root systems of the seedlings obtained by breeding is at least five, the average length of main root and lateral root is at least 5cm greater than that of the conventional seedling breeding, the stem width is 0.8 to 1.2cm, and the full bud number of a training belt is more than 10. The plum and apricot seedlings bred by the invention have developed root systems, are robust and non-toxic, and have high survival rate and early production period.

The invention discloses a planting method of lithocarpus polystachyus. The planting method comprises the following steps of soil preparation, tillage of planting rows, lifting of seedlings, planting, young forest management, pruning and forest establishment management. After lifting of seedlings, a nutrient solution is used for being mixed with red-yellow adhesive to be slurry for root dipping, moisture is preserved, and survival of seedlings is ensured; before planting, fermented biological organic fertilizer is applied so as to provide comprehensive nutritive elements for the seedlings before planting, wherein the biological organic fertilizer is fermented chicken manure and pig manure, contains no harmful bacteria, is easy to absorb and does not burn seedlings; during planting, seedlings with the large root number and obvious side roots are selected, and the well-developed side roots are beneficial to rapid recovery and development of root systems of the seedlings and beneficial to improvement of the survival rate of afforestation; and during topdressing, biological organic fertilizer special for tea leaves is used, soil hardening is avoided, tender leaves of tea trees are increased, and the yield and quality of tea leaves are effectively improved.

The invention belongs to the technical field of vegetative propagation of plants and particularly relates to a rapid propagation method of callicarpa nudiflora. The method comprises the following steps of 1 preparing ear seedlings; 2 preparing a mixed matrix; 3 preparing and cutting cuttings; 4 performing later period management and maintenance. By means of the rapid propagation method, the time required for rooting can be effectively shortened, the rooting percentage is obviously improved, the root number and the maximum root length are obviously increased, and the root slanting rate is reduced. The seedling emergency percentage of produced nursery stocks and survival rate of transplanted nursery stocks are high. Good characters of stock plants can be kept stable and reliable by means of the rapid propagation method. The rapid propagation method is low in cost and easy to popularize technologically and has wide application prospect.

The present invention discloses a blueberry softwood cutting seedling method. The method comprises the following steps: selecting and trimming cutting woods, soaking the trimmed woods with a rooting agent containing boron and IBA for 8-10 hours, and inserting the woods into a matrix, wherein the humidity of the matrix is kept at 80% or above, and the temperature is controlled at 20-28 DEG C. The method of the present invention is simple to study, high in operability, good in rooting effect and high in utility value, wherein the rooting rate reaches up to 91.18%, so that the callus formation rate and the rooting number can be significantly improved.

The invention provides a new method for tissue culturing chrysanthemum. It employs cold light source (light-emitting diode) as light source, and determines the optimum ratio of red light to blue light and the best light emitting strength. It takes poromeric fluoroethylene resin film as culturing container for tissue culture sprout; and employs rock fiber block as culture medium. The growing speed for tissue culture sprout is one time faster than that with normal culture method. The tissue culture sprout and replanting sprout are characterized by largest plant height, largest leaf number, root number, longest root length and largest dry net weight of haulm and root, high rate of emergence and little pollution rate.

The invention discloses application of amino acid transport gene OsAAP1to promotion of rice growth in low nitrogen, belonging to the field of plant genetic engineering. Amino acid of the OsAAP1 gene coding protein and cDNA sequence thereof is shown as following SEQID NO.1, 2. The rice root length, root number, plant height and tiller number can be increased and biomass and yield can be improved by improving the OsAAP1 gene expression in low nitrogen through establishing the rice OsAAP1 overexpression plant. The rice root length, root number, plant height and tiller number can be reduced by decreasing the OsAAP1gene expression through establishing interfering plants. Thereby, the OsAAP1gene can be applied in improving the biomass and the yield of the rice in low nitrogen. The OsAAP1 gene has important application in the respect of rice tolerance to low or poor soil or farmland, reduction of chemical fertilizer usage amount and the rice breed improvement.

The invention discloses a soilless culture substrate for balsamine, which belongs to the field of plant culture substrates. The soilless culture substrate for balsamine is characterized by consisting of pindstrup seedling culture substrate soil (0 to 10 millimeters) and perlite in a volume ratio of 2.5:1 to 3.5:1. Compared with the prior art, the substrate has the advantages that the defects of poor moisture retention of vermiculite in the common culture substrate and insufficient absorption of nutrient fluid are overcome, and the plant height, the root length, the root number and the stalk thickness of the balsamine are remarkably increased.

The invention discloses a soilless culture substrate for violet, which belongs to the field of plant culture substrates, in particular to the field of soilless culture substrates for violet. The substrate is characterized by consisting of peat cube, perlite and coconut husk in the volume ratio of (2-3.5):1:1. The soilless culture substrate is suitable for growing violet, makes a seedling grow strongly with a large amount of large and order leaves, meets the growing requirement of violet to a large extent, and remarkably promotes the remarkable increase of violet in the aspects of plant height, root length and root number.

The invention discloses a Nuccio's bella rosa camellia cutting seedling-raising method. The method includes the following steps: selecting red soil as a cuttage medium; selecting annual strong healthy semi-lignified branches without disease and pests as scions in the middle-last ten days of June, and processing the scions with a rooting agent, and performing cuttage, wherein the cuttage density is 4 cm X 8 cm; covering a greenhouse with a sunshade net after cuttage, controlling the temperature of the greenhouse at 20-30 DEG C and the humidity of the green house at 70-80% by folding and unfolding the sunshade net and ejecting water, and performing disease and pest control; and allowing the scions to take roots in 1 month after cuttage, transplanting the rooted seedlings to nutriment pots after 2 months, performing seedling training for 3 months, and transplanting the seedlings to a field. According to the characteristics of the Nuccio's bella rosa camellia, the red soil is used as the cuttage medium, 600-1000 ppm of 3-Indolybutyric acid is used to process the scions, twig cuttage seedling raising is performed in a small arc shed, the average root length and the root number of the cuttage seedlings of the Nuccio's bella rosa camellia can be remarkably increased, and the survival rate of the transplanted seedlings is greatly improved.

The invention discloses an application of an amino acid transport gene OsAAP5 to rice breeding, and belongs to the field of plant gene engineering. An amino acid sequence of a protein encoded by the gene OsAAP5 is shown in SEQ ID NO.1, and a cDNA sequence is shown in SEQ ID NO.2. By constructing the rice gene OsAAP5 for plant interference, it is discovered that the root length, root number, plant height and fresh weight of normal rice can be increased by reducing expression of the gene OsAAP5, so that the gene OsAAP5 can be used in the rice breeding to facilitate rice growth and increase rice biomass. The gene OsAAP5 has an important application value in the aspect of describing the influence of amino acid transport on the plant growth and development process.

The invention discloses a preparation method suitable for a gardenia cutting rooting agent. The preparation method comprises the steps: eggshell pretreatment, juice calcium preparation and gel rooting agent compounding. The preparation method has the beneficial effects that domestic garbage eggshells serve as a calcium source to be processed and utilized, so that not only is the environment protected, but also the full utilization of a material is realized; the rooting agent contains medium trace elements and nutrient substances such as auxin, a microbial inhibitor, organic matters, nitrogen, phosphorus, potassium, calcium, magnesium and zinc so as to be beneficial to cutting rooting and growth; and a gel rooting agent wrapped on the surface of a whole cutting forms a layer of rooting agent film which has water retention and moisture retention effects, the gel rooting agent film on the ground can be used for reducing evaporated water of the cutting and preventing the cutting from withering and having lesions, and the gel rooting agent film under the ground is kept in a wet state because of absorbing water from a cutting substrate and also slowly releases a rooting agent to stimulate the cutting to root, so that the rooting number, root length and robustness of the cuttings is increased, and furthermore, the survival rate of the cuttings is increased.

The invention discloses a method for tissue culture and rapid propagation of cortex acanthopanacis, namely a method for obtaining a large number of good cortex acanthopanacis nursery stocks within a short period of time by means of the plant tissue culture technique. A cortex acanthopanacis stem with buds is used as explant, in-vitro regeneration of cortex acanthopanacis is achieved through shoot induction, shoot enrichment culture, rooting culture, seedling hardening, transplantation and the like, and technical support is provided for mass breeding, rapid propagation and new species popularization. The method has the advantages that propagation and growth are fast, root number is large, roots are dense, seedlings are robust, drug property is strong and efficacy is excellent.