64 results about "Taproot" patented technology

The invention relates to the technical field of soil improvement and ecological restoration, and discloses a liquorice planting method based on synergistic utilization of coal-based solid waste and desertified land, which comprises the following steps: crushing alkaline coal-based solid waste and acidic coal-based solid waste, mixing according to a mass ratio of 2: 8-4: 6, adding a modified humic acid-based heavy metal passivator and water, and uniformly mixing to obtain a mixture; uniformly mixing and aging to obtain a coal-based solid waste mixture; spreading the mixture on the surface layer of the desertification land, performing rotary tillage and deep tillage to mix the mixture with the sandy soil, and adjusting the pH value to be stable at 7.5-8.5; the treated liquorice seeds are sown, main roots are harvested in autumn of the third year, deep root systems and lateral root bud points are reserved, and overground straw is smashed and returned to the field; soil nutrition indexes are monitored regularly, other commercial crops are planted when the soil nutrition indexes reach a set threshold value, and if not, regeneration planting of the liquorice is maintained. Through synergistic compounding of acid-base solid waste and chelating of the passivating agent, substrate pH self-regulation and heavy metal resistance control are achieved, and the physicochemical property of sandy soil is improved.

The invention belongs to the technical field of plant genetic engineering and disease-resistant breeding, and particularly relates to a construction method of soybean GmLNK2 gene four mutants and application of the soybean GmLNK2 gene four mutants in soybean disease-resistant breeding, by means of a CRISPR / Cas9 technology, wild soybean WS82 is used as a material, three target spots are designed for GmLNK2a, GmLNK2b, GmLNK2c and GmLNK2d genes of a GmLNK2 gene family, and simultaneous knockout is performed to obtain a mutant plant. Compared with a wild type WS82, the plant height of a mutant plant is obviously reduced, the length of a main root is obviously shortened, and the leaf area of three-out compound leaves is obviously reduced, which indicates that the mutant plant is more sensitive to pathogenic bacteria in a root rot disease nursery, the susceptibility degree is more serious, and growth and development are obviously inhibited, the key effect of the GmLNK2 gene family in soybean root rot resistance is clarified, and the application of the GmLNK2 gene family in soybean root rot resistance is developed. Important gene resources and technical support are provided for soybean disease-resistant molecular mechanism research and disease-resistant variety cultivation.

The invention relates to the technical field of fruit tree cultivation, and discloses an apple deep-rooted cultivation method suitable for M9T337 rootstock, the apple deep-rooted cultivation method comprises the steps of nursery stock pretreatment, ditching and field planting, staged soil covering and chemical control management, rootstock cortex rooting is induced by combining layer-by-layer soil covering and chemical control measures, and the problems of shallow root system and poor stress resistance of the M9T337 rootstock are solved. According to the method, a complete technical chain of pretreatment, field planting, root inducing, regulation and maintenance is constructed, a continuous deep root induction environment is created through staged soil covering, and a deep root cultivation mode from passive adaptation to active induction is realized by matching with pretreatment of a composite agent to induce rooting and chemical control management to coordinate nutrient distribution. Practice shows that after M9T337 rootstock apple seedlings cultivated through the method are planted for one year, the proportion of deep root systems below 30 cm is increased to 45-50% from 15-20% of traditional cultivation, the length of main roots can reach 50-60 cm, root system distribution is more reasonable, and the deep water and fertilizer absorption capacity is remarkably improved.

The invention discloses an application of an OsCFIm68b gene in regulation and control of Cd resistance of rice and Cd accumulation of grains. The OsCFIm68b gene in rice is knocked out through gene editing to obtain a knockout mutant, and it is found that under the Cd polluted soil culture condition, the length of a main root is shortened, the number of lateral roots is reduced, the length of a total root is shortened, and the Cd concentration of grains is reduced to 1 / 3 of that of a wild type. Therefore, the OsCFIm68b gene is a negative regulatory factor for encoding Cd accumulation of the rice grains, the Cd accumulation of the rice grains can be improved by weakening the expression of the OsCFIm68b gene, the Cd resistance of the rice is improved, and a new gene target is provided for Cd low-accumulation breeding of the rice.

The invention discloses a container seedling raising method using chaff to assist in root cutting, and belongs to the technical field of container seedling raising. Aiming at the technical pain points that root systems easily break through containers, seedlings are difficult to take and the survival rate is low in existing container seedling raising, the method comprises the steps of laying part of chaff in a nursery, adding the chaff into cup filling soil, guiding the root systems to extend in multiple directions by utilizing the chaff after the containers are placed, filling gaps among cups with the chaff and the like, so that the growth of the root systems is physically regulated and controlled. According to the method, more fibrous roots and less main roots can be generated on seedling roots, so that the seedling taking efficiency is remarkably improved (the seedling taking time is shortened by about 30%, and the seedling taking cost is reduced by about 30%), the seedling survival rate is improved (improved by 3%), and the transportation cost is reduced (reduced by about 15%). Although the seedling raising cost is increased by 3-5%, comprehensive benefits are remarkable, good social benefits and economic benefits are achieved, and the method is suitable for large-scale container seedling raising production of various seedlings such as cedarwood and slash pines; the method has particularly obvious social benefits and economic benefits for culturing cup seedlings of tree species with obvious main roots, such as cyclobalanopsis rubra, cyclobalanopsis glauca, liquidambar formosana hance, phoebe zhennan and the like.

The application relates to the technical field of forestry seedling raising and ecological restoration, and discloses a root guiding type full-biodegradable tree planting bag based on hybrid material modification and application, the tree planting bag is an integrally injection molded structure, the inner wall is provided with vertical root guiding edges, and the bottom is provided with a cross-shaped easy-to-crack line; the tree planting bag is longitudinally divided into an upper structure area and a lower degradation promoting area. The upper part is composed of PBAT / PLA and hybrid materials and has high strength and weather resistance; the lower part is composed of PBAT / PPC, hybrid materials and plant fibers and has moisture absorption and rapid degradation characteristics. The hybrid material is a composite in which nano-hydroxyapatite grows in situ on the surface of attapulgite. Through the synergy of the longitudinal gradient material and the physical root guiding structure, long-term shape retention during seedling raising and rapid disintegration of the bottom after planting are realized, the problems of seedling taproot and degradation lag are solved, the degradation products can adjust the pH value of the rhizosphere soil and provide phosphorus and calcium nutrition, and the afforestation survival rate is improved.

This utility model relates to the field of soybean planting technology, specifically disclosing a soybean planting box, including a base, a water collection tank fixedly connected to the upper end of the base, multiple planting box bodies arranged above the water collection tank, an adjustment mechanism arranged between the multiple planting box bodies, multiple evenly distributed conical protrusions arranged at the bottom of the inner wall of each of the multiple planting box bodies, and multiple drainage holes arranged alternately with the conical protrusions through the lower end of each of the multiple planting box bodies, and planting substrate filled in each of the multiple planting box bodies. By setting the conical protrusions at the bottom of the inner wall of the planting box body to form a three-dimensional slope, the main root of soybean is guided to grow down along the sloping surface of the protrusion, while the lateral roots extend laterally from the gaps between the cones, avoiding the root entanglement caused by the traditional flat bottom wall. The conical structure makes the bottom wall surface form a non-planar drainage channel, and excess water flows quickly to the surroundings along the sloping surface of the cone and is discharged from the drainage holes, preventing soybean roots from rotting due to waterlogging.

This invention discloses Chinese kale BoaFLZ35 The application of genes in promoting vegetative growth of plants. This invention utilizes a gene-based approach to construct a *Gynostemma pentaphyllum* plant. BoaFLZ35 Heterologous overexpression of the gene in Arabidopsis thaliana was used to observe and analyze its effects on vegetative growth traits such as root elongation and rosette leaf morphology in transgenic plants. The results showed that heterologous overexpression... BoaFLZ35 This gene can significantly increase root cell length, thereby promoting the elongation of the Arabidopsis taproot; simultaneously, it can also increase leaf cell volume, resulting in significantly larger rosette leaves in transgenic plants. Therefore, it is evident that... BoaFLZ35 This is an important gene in the FLZ family of plants that positively regulates vegetative growth. This invention provides new insights into the function of FLZ family genes in the genus Brassica and also provides important candidate genes for high-yield molecular breeding of Brassica vegetables such as Chinese kale.

This invention discloses a deep-sea Bacillus strain and its applications, belonging to the field of agricultural microbiology and biocontrol technology. This invention screened and identified a deep-sea Bacillus strain NT-3-7. Experimental verification showed that the deep-sea Bacillus strain NT-3-7 provided by this invention is tolerant of saline-alkali environments (it can grow in 15% NaCl and pH 9.0 environments) and possesses good nitrogen-fixing, phosphorus-solubilizing, and potassium-solubilizing abilities; it has excellent growth-promoting effects on plants, increasing plant height, taproot length, root dry weight, and stem and leaf weight of chili pepper plants. Using the deep-sea Bacillus strain NT-3-7 provided by this invention to prepare products that promote plant growth can help promote sustainable agricultural development while significantly reducing environmental pollution, showing promising application prospects.

This utility model discloses a layered mesh integrated cultivation pot, relating to the field of cultivation pot technology. This layered mesh integrated cultivation pot includes a substrate pot, a lower trapezoidal sand storage pot, and an integrated structure. The lower trapezoidal sand storage pot is fixedly connected to the bottom of the substrate pot, with a ratio of 1:2 between the substrate pot and the lower trapezoidal sand storage pot. The integrated structure includes a mesh, a bottom plate, a feeding port, a rectangular support plate, a cultivation frame, and a spacing plate. The cultivation frame is mounted on the substrate pot, and the bottom of the cultivation frame is integrally formed with a spacing plate. Multiple sets of horizontal holes are provided on the spacing plate to facilitate the growth of seedling roots. Compared with traditional root-promoting cultivation methods, this utility model rationally separates the upper and lower layers. The substrate pot has ideal bulk density and good physicochemical properties, which is beneficial for fixing seedlings or miniature potted plants to maintain upright growth, ensuring normal root development, and achieving simultaneous root strengthening, root promotion, and root nourishment, reducing manpower, material resources, and financial resources during the root promotion period.

The invention discloses an intelligent control method and system for water and fertilizer of under-forest crops based on Internet of Things sensing, and relates to the field of agricultural intelligent equipment. The system comprises a sensing layer, a transmission layer, a data processing layer, an execution layer and an interaction layer, the sensing layer collects soil nutrients, humidity and environmental parameters by deploying multi-parameter sensing nodes (avoiding a tree main root area) in a matrix manner, and transmits the soil nutrients, humidity and environmental parameters to a cloud platform through dual-mode communication; the data processing layer calls a water and fertilizer regulation and control model fusing an expert rule base and an improved BP neural network, and generates a regulation and control scheme in combination with a crop growth stage; the execution layer achieves accurate application through zoned drip irrigation and flow feedback, and closed-loop adjustment is formed. The system solves the problems of monitoring distortion, extensive decision, unstable communication and the like in the prior art, realizes intelligent and accurate regulation and control of water and fertilizer of understory crops, improves the utilization efficiency of water and fertilizer, and adapts to a complex understory environment.

This application discloses an ecological slope protection fiber blanket that combines root-based biomimetic anchoring with microbial grouting, relating to the field of slope ecological restoration technology. It includes: a stress-resistant seed water-retaining layer, a microbial microcapsule slow-release layer, and a biomimetic fiber mesh layer. The stress-resistant seed water-retaining layer comprises an upper protective net, a water-retaining nutrient layer, and a bonding layer, arranged sequentially from top to bottom. The microbial microcapsule slow-release layer is located below the stress-resistant seed water-retaining layer and includes a solidified microbial community and a microcapsule embedding layer, with the solidified microbial community filling the microcapsule embedding layer. The biomimetic fiber mesh layer is located below the microbial microcapsule slow-release layer and includes a taproot, fibrous roots, and microroots, which work together to form a biomimetic fiber mesh structure. This application achieves both short-term ecological restoration and long-term stability balance of slopes.

This application relates to the field of agricultural irrigation and soil water and salt management technology, and discloses a method for coordinated regulation of water and salt in the root zone of jujube trees based on continuous vertical pipe irrigation. The method includes: deploying vertical pipes around the main root zone of the jujube tree to continuously supply slightly saline water; obtaining meteorological theoretical water consumption demand and headstock observation flow rate, identifying effective infiltration flow rate and calculating the total external salt mass; constructing surface state quantities, transient transitional inventory in the main root protection zone, and deep state quantities, disassembling the total external salt mass and updating the above state quantities; calculating the freshwater maintenance capacity factor and isolation integrity index, obtaining surface overload contribution items and deep overload contribution items; when the isolation state of the main root zone is determined to be ineffective, comparing the surface overload contribution item with the deep overload contribution item, and outputting a matching hydraulic regulation command. This invention overcomes the defect of soil sensors being prone to corrosion and failure, improves the accuracy of salt distribution measurement, achieves targeted hydraulic remediation, and avoids secondary hydrological hazards caused by flood irrigation.

This invention provides an indirect tensile strength testing method for plant roots using a splitting method, belonging to the field of plant root strength testing technology. This invention draws on the indirect tensile principle of the Brazilian splitting test for rocks, employing a three-point bending splitting method to replace the traditional direct tensile method. The steps include specimen preparation, sample fixing, loading test, strength calculation, and data screening. A single taproot is selected as the specimen, and the equivalent diameter is determined using a cross-shaped bidirectional diameter measurement method. The specimen is placed horizontally on two concave-facing arc-shaped rubber supports. Vertical pressure is applied to the midpoint of the specimen using a convex-facing indenter. The peak load is used as the failure load, and the test is terminated when the load decreases by 20%. The equivalent tensile strength is calculated using an approximate formula for rectangular cross-sections. This invention is simple to operate, has a high success rate, is suitable for the irregular circular cross-section characteristics of plant roots, allows for batch testing, and the test results closely approximate the actual stress state of the root system. It can be widely applied to root strength testing in engineering and scientific research.

This invention discloses a peony PsLBD12 Genes and their application in regulating root development PsLBD12 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsLBD12 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into the target plant to verify its function. It was found that overexpression... PsLBD12 Compared with wild-type Arabidopsis seedlings, the Arabidopsis seedlings with this gene grew faster, exhibited earlier stem elongation, and showed earlier senescence of basal leaves in the later stages of growth. The taproot length was significantly shortened, the number of lateral roots was significantly increased, and the endogenous hormones IAA and ABA in the leaves were significantly increased. The average number of roots in detached leaves was significantly increased on MS medium containing IAA, and the average root length was significantly increased on MS medium containing IBA. It is evident that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

This invention relates to the field of forest tree cultivation technology, and in particular to an efficient cultivation method and application for oak trees with well-developed taproots, comprising the following steps: S1, Standard seedling cultivation: Oak seedlings are cultivated using a biodegradable non-woven fabric seedling container filled with a seedling substrate composed of a lightweight matrix and a compound controlled-release fertilizer; S2, Mixed gel preparation: A water-retaining agent, microbial agent, and slow-release fertilizer are mixed with water to prepare a water-fertilizer-bacteria mixed gel; S3, Planting operation: Planting holes are dug at the planned location. A predetermined amount of the water-fertilizer-bacteria mixed gel is first applied to the bottom of the hole. Then, the roots of the standard seedlings cultivated in step S1 are dipped in the mixed gel and placed in the planting hole. Soil is backfilled and compacted; a surrounding dike is built around the seedlings. The biodegradable non-woven fabric container of this invention fixes the root system and prevents tangling during the seedling stage. After planting, it degrades without forming an obstacle, achieving truly whole-root, damage-free transplanting. The lightweight matrix creates a loose and breathable ideal environment for root development.

The application discloses a traditional Chinese medicinal material transplanting and fertilizing device with seedling separating and supporting functions, and particularly relates to the technical field of traditional Chinese medicinal material fertilizing, and comprises a groove plate, an electric cylinder is fixedly installed on one side of the outer wall of the groove plate, and a root protection fertilizing assembly is arranged at the output end of the electric cylinder, wherein the root protection fertilizing assembly comprises a connecting block and an arc-shaped inserting plate; the connecting block is fixed at the output end of the electric cylinder, and the electric cylinder is used for pushing the connecting block to move downward; and the arc-shaped inserting plate is fixed on one side of the connecting block. The root protection fertilizing assembly has the advantages of synchronous protection of the main root periphery and the horizontal side part and precise fertilizing, effectively avoids error damage caused by manual operation during multi-dimensional root fertilizing, and greatly reduces the damage rate of root injection fertilizing, so that the problem that the main root area of the traditional Chinese medicinal material is easily damaged due to excessive or wrong deviation insertion and the damage rate of root injection fertilizing is high is solved.

The application discloses a non-woven fabric light substrate deep-root seedling raising method, which comprises the following steps: step 1, wrapping plant seedlings and a second substrate layer in an inner covering film to form an insertion core; the inner covering film is wood pulp non-woven fabric or cotton pulp non-woven fabric; step 2, using a perforating tool to form an insertion hole in the middle of a first substrate layer of a non-woven fabric seedling raising container, and inserting the insertion core prepared in step 1 into the insertion hole, so that the non-woven fabric seedling raising container and the insertion core form a seedling raising unit; the non-woven fabric seedling raising container comprises an outer covering film and a first substrate layer located in the inner part of the outer covering film, and the outer covering film is non-woven fabric treated by an organic silicon hydrophobic agent; step 3, transporting the prepared seedling raising unit to a cultivation tray in a nursery; and performing water and fertilizer management on the plant seedlings according to set water and fertilizer conditions. The non-woven fabric light substrate deep-root seedling raising method can avoid the problems of root spiral and burrowing, promote vertical deepening of taproots, and improve the afforestation survival rate in arid, semi-arid and desert areas.

The application belongs to the technical field of plant genetic engineering, and particularly relates to application of a PoPILS2a gene in promoting plant root development. The application finds that overexpression of the PoPILS2a gene can significantly promote the main root elongation and lateral root development of peony, and improve the overall root architecture, indicating that the PoPILS2a gene plays an important positive regulation role in the root development of peony. The application lays a solid theoretical foundation for future directional improvement of the peony root through genetic engineering means and improvement of the medicinal and ornamental values of the peony.

This invention discloses a method and application for promoting root bending and lateral root formation in seed-sown persimmon (Diospyros kaki). This invention belongs to the technical field of persimmon seedling cultivation and bonsai material cultivation. The method includes low-temperature stratification treatment of persimmon seeds; disinfection and imbibition treatment of the stratified seeds followed by sowing in a gibberellin-containing GA3 medium; periodic artificial adjustment of the radicle growth direction when the radicle reaches 1 cm in length to induce taproot bending; removal of 1 cm of root tip when the taproot reaches 4 cm in length to promote lateral root and fibrous root germination; and subsequent hardening-off and transplanting. This invention, by regulating early geotropism of the radicle and taproot apical dominance, enables the taproot of persimmon seed-sown seedlings to form a natural bending shape and significantly promotes lateral root germination, resulting in good ornamental value, growth vitality, and promising application prospects.

The application belongs to the field of biotechnology and particularly relates to ABC110 gene and application thereof in improving natural rubber yield of guayule. A nucleic acid molecule is provided, and the nucleotide sequence of the nucleic acid molecule is shown in SEQ ID NO:1 or SEQ ID NO:2. The application also provides application of the nucleic acid molecule in cultivating guayule plants with improved root biomass and natural rubber content. Overexpression of the nucleic acid molecule in guayule can significantly increase the taproot diameter of guayule, increase the dry root weight of each guayule plant and improve the natural rubber content of the roots of guayule. The application provides a new gene target and method for genetic improvement of guayule and has a wide application prospect in cultivation of high-yield guayule varieties.

The application relates to a method for efficiently propagating plants through long-beaked thick-embryonic cells, and belongs to the technical field of plant tissue culture. The method comprises five steps of embryonic cell induction, proliferation and maintenance, somatic embryo differentiation and maturation, germination and seedling growth and domestication and transplanting. By optimizing the sucrose concentration in the induction medium to 50 g / L and adopting the technology of combining cell cluster sorting with suspension culture, the induction rate and the proliferation speed of the embryonic cells are significantly improved; by controlling the inoculation density and adding glutamine and abscisic acid in combination during the somatic embryo differentiation stage, about 67 cotyledon embryos can be generated from 200 cell clusters; during the germination stage, by synergistically using gibberellin and Karrikin 1, the synchronous development of roots and stems is promoted, and robust seedlings with multiple true leaves and multiple main roots are obtained. The application first realizes the large-scale propagation of about 6800 somatic embryo seedlings from 1 gram of embryonic cells, and the transplanting survival rate reaches 96.2%, thereby providing an innovative technical scheme for the germplasm preservation and efficient propagation of long-beaked Magnolia officinalis.

This invention relates to the field of agricultural planting technology, specifically disclosing a method for increasing quinoa yield by improving drought and lodging resistance. The method includes: acquiring quinoa taproot growth data within a historical planting monitoring period, analyzing the root growth data for obstruction to determine the periods of root obstruction, analyzing changes in soil compaction data within the historical planting monitoring period to determine the periods of soil compaction, and comparing these periods with those of root obstruction to determine whether the obstruction is caused by soil compaction. This method can accurately pinpoint key factors affecting quinoa growth, avoiding unnecessary measures and improving the efficiency and accuracy of planting management. Furthermore, in periods and areas where soil compaction is more pronounced, concentrated human, material, and financial resources can be invested in soil improvement, while in other periods and areas, resources can be used for other aspects beneficial to quinoa growth, rationally allocating planting resources and improving resource utilization efficiency.

The invention relates to the field of food processing, in particular to a preparation method of whole-plant fried ginseng and application of the whole-plant fried ginseng in preparation of drugs or food for resisting oxidation and relieving fatigue. The method comprises the following steps: fresh ginseng is taken and cleaned, rhizome, main roots and fibrous roots are separated, the main roots are cut into slices or segments, the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are respectively placed in oil to be fried, and when the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are golden yellow, the rhizome, the main roots and the fibrous roots are fished out, drained off oil and cooled. The antioxidant activity of the fried ginseng slices and segments is improved. Compared with fresh ginseng, the deep-fried ginseng slices and segments can significantly prolong exhaustive swimming time of load-bearing mice, significantly improve liver / muscle glycogen, lactic dehydrogenase content and superoxide dismutase activity, reduce lactic acid, urea nitrogen and malondialdehyde content, and have a significant anti-fatigue effect. According to the method, dryness of fresh ginseng can be removed, the eating fragrance can be improved, the fried ginseng product is instant and convenient to eat, and the fried ginseng product is matched with barbecue kebabs and stewed together with livestock and poultry meat, so that the fried ginseng product is delicious and health-care.

The invention discloses a transplanting, seedling raising and afforestation method. The method comprises the following steps: S1, selecting seedlings; s2, bag preparation: preparing Meijing bags, wherein the size of the Meijing bags is matched with that of the soil balls after the seedlings are lifted; s3, line drawing, wherein a circle is drawn on the ground around the trunk with the center of the trunk as the circle center and the radius of the beauty-woven bag as the radius; s4, seedling digging, wherein 1-5 main roots of each psammophyte are left as reserved roots; s5, bagging is conducted, specifically, the bottoms of the psammophyte seedlings are sleeved with American woven bags; s6, preparing a seedling raising substrate: sprinkling water on sand, coal gangue powder, mudstone powder and dry branch and fallen leaf powder until dust is not generated, and uniformly stirring to obtain the seedling raising substrate; s7, filling, wherein gaps between the American woven bags and the root systems of the psammophyte seedlings are filled with the seedling raising substrates, and leveling is conducted; s8, supporting is conducted; s9, maintenance is conducted; s10, root cutting, wherein after the psammophyte seedlings are in half of the growing season, part of the psammophyte seedlings are cut off, and roots are reserved; s11, transplanting is carried out; by the adoption of the technical scheme, the cost, the survival rate and the rejuvenation period of the method are far better than those of traditional seedling cultivation and afforestation.

The invention discloses application of bacillus pumilus YYZB12 in growth promoting cultivation of medicinal rhubarb, and belongs to the technical field of microorganisms. The bacillus pumilus YYZB12 strain is separated from rhizosphere soil of medicinal rhubarb in Zhenban county in Han City of Shaanxi Province, namely a genuine production area of medicinal rhubarb, and is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.35249. The strain has efficient phosphate solubilizing capacity, can promote the thickening growth of the main root of the medicinal part of the medicinal rhubarb, and obviously improves the accumulation of anthraquinone secondary metabolites at the root of the medicinal rhubarb. An excellent microbial strain resource is provided for ecological planting of the medicinal rhubarb, the method can be applied to artificial cultivation of the medicinal rhubarb, the medicinal material quality and the commodity value of the medicinal rhubarb are effectively improved, and the method has important significance in promoting reasonable application of a microbial technology in standardized planting of medicinal plants.

The invention belongs to the field of gene engineering, and particularly relates to application of a rice gene to improvement of acid-resistant planting of rice. According to the invention, the acid resistance of rice is improved by adopting the OsSTOP1 gene or the encoded protein thereof; wherein the OsSTOP1 gene belongs to an STOP1 transcription factor family, the homology of the OsSTOP1 gene and AtSTOP1 of arabidopsis thaliana reaches 54.8%, and the OsSTOP1 gene contains a conservative C2H2 zinc finger structural domain. According to the invention, an overexpression vector driven by an OsSTOP1 self promoter is constructed, and a rice Nipponbare variety is transformed by using an agrobacterium-mediated method, so that a T0-generation transgenic positive plant is obtained. QRT-PCR (quantitative reverse transcription-polymerase chain reaction) and Westernblot verification show that the OsSTOP1 is remarkably overexpressed in a transgenic plant. Acid resistance experiments show that OsSTOP1 enhanced expression can significantly relieve the inhibition effect of acid stress with the pH value of 4.8 on growth of main roots of rice, the length of the main roots of a wild type is reduced by 26.8%, and OE-OsSTOP1-6 and OE-OsSTOP1-8 are only reduced by 18.7% and 20.1% respectively. The invention provides a key gene resource and an efficient screening method for acid-resistant molecular breeding of rice, and has important agricultural application value.

The application provides a rapid and high-quality breeding method of Vitis quinquangularis, which comprises six steps of selection and treatment of cuttings, disinfection of cuttings, root induction in an electric hotbed, preparation of a substrate, cutting seedling and transplanting. The single-bud pruning method of the application can obtain the most cuttings, the use of a compound solution of indole acetic acid and indole butyric acid as a rooting agent can make the taproot and lateral roots germinate at the same time, and the growth speed is increased by more than 50%, the application also uses a fermentation substrate nutrient bag for cutting seedling, which ensures balanced nutrition supply, the seedling rate can reach more than 98%, the survival rate can reach more than 95%, and the breeding cycle is short.

This invention relates to the field of plant root division technology, and more particularly to a root division method for root zone-root zone communication research and its application. The root division method of this invention includes the following steps: (1) Seedling cultivation: germinating seeds are hydroponically cultured to obtain seedlings; (2) Main root pruning of seedlings: when the lateral roots of the seedlings described in step (1) grow to 3-4 cm, the main root is pruned to obtain seedlings with pruned main roots; (3) Double rootstock grafting of seedlings: two seedlings with pruned main roots of the same growth are selected for double rootstock grafting to obtain grafted seedlings; (4) Grafting of grafted seedlings: the roots of the grafted seedlings are completely immersed in the culture solution, with the grafting interface outside the culture solution, and cultured until the grafting interface is completely healed and both rootstock A and rootstock B have grown new roots, to obtain seedlings to be divided; (5) Root division: the seedlings to be divided are transferred to a root division box for root division treatment. The root division method of this invention can more accurately and intuitively reflect the exchange of substances between roots.

The invention discloses a speed-controllable and passively-driven self-adaptive plant-root-sheath-imitating anchoring device, and relates to the technical field of planetary detection, and the device comprises a detector main body, a buffer landing leg is installed on the circumference of the middle part of the detector main body, a foot pad is installed at one end, away from the detector main body, of the buffer landing leg, and a main root drill bit is arranged in the middle of the foot pad; the speed-controllable and passively-driven self-adaptive plant-root-sheath-imitating anchoring device further comprises a dynamic regulation and control mechanical structure and a multifunctional stretching control mechanism. The dynamic regulation and control mechanical structure is arranged in the main root drill bit and is used for fixing and adjusting the foot pad; the device adopts a plant root sheath imitating design, adapts to weak-gravity loose surfaces such as asteroid gravel piles and the like, is high in anchoring force, light in weight and good in controllability, can ensure that a detector stably executes in-situ detection and sampling tasks, and provides a reliable anchoring scheme for planetary detection.