32 results about "Hereditary property" patented technology

The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seed storage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-tea camellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.

The invention relates to a breeding method of a common mudflat shellfish with stress resistance and high yield, particularly a method of adopting second instar wild type mudflat shellfish as parent to establish a sib family and selecting one family with fastest growth, highest survival rate and highest rate from the first filial generation. By choosing excellent ones for breeding from the first filial generation, the groups of two families with fastest growth and highest survival rate are carried out cross-breeding, the fine strain with highest yield is chosen which is carried out the above breeding, and finally the fine variety with stress resistance and high yield is obtained. The advantage of the method is that the genes with economic character in the family can be purely combined fast, thus obtaining a new variety with high yield, stable yield and stress resistance. The family material can be used for study the hereditary property of mudflat shellfish so as to construct the gene linkage map of the mudflat shellfish.

The invention relates to a method for the quick seedling raising of the seeds of shinyleaf yellowhorn, which belongs to the planting of fruit trees. The method for sowing seeds and raising seedlings in nursery lands with sand soil comprises the following breeding steps: seed collection, seed selection, stratification, nursery land selection, spring sowing and nursery management. The invention hassuperior biological hereditary property, no virus accumulation, flourishing root system, robust tree seedlings and high survival rate. The plant height of the tree seedlings of the raised shinyleaf yellowhorn out of the nursery is 70-100 centimeters, the length of taproots is 20-30 centimeters and the survival rate is more than or equal to 90%. The invention provides enough tree seedlings for functions in the aspects of planting shinyleaf yellowhorn trees in a large amount, exerting the economic benefit, the social health-care benefit and the medical treatment benefit of the shinyleaf yellowhorn trees, preventing sand, consolidating soil and protecting the environment. The invention is suitable for the quick seedling raising of the seeds of various yellowhorn variation varieties.

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

The invention discloses a Pseudomonas aeruginosa strain SWJSS3 and application thereof in producing proteinase. The strain is collected by General Microorganism Center of China Committee for Culture Collection of Microorganisms on June 10th, 2015; the collection address is Institute of Microbiology in Chinese Academy of Sciences, Yard 1, Beichenxi Road, Chaoyang District, Beijing City; and the collection number is CGMCC No.10973. The application method of the strain for producing proteinase comprises the following steps: adding a fermentation culture medium into a fermentation tank, wherein the liquid filling amount is 8-12%, the initial pH value is 7.0-7.5, the strain inoculum size is 0.8-1.2%, and the initial temperature in the tank is 20-40 DEG C; and fermenting under oscillating conditions for 30-80 hours to generate abundant proteinase in the tank, and separating and extracting to obtain the proteinase. The strain SWJSS3 has high proteinase production capacity; and the obtained proteinase has the characteristics of high enzyme activity and high salt tolerance. The fermentation method disclosed by the invention has the advantages of simple conditions and stable hereditary property, and is suitable for industrial production.

The invention relates to a susceptibility gene region closely related to psoriasis and a method for detecting psoriasis susceptible population. The susceptibility gene region contains 7 genes, that is, LCE3A, LCE3B, LCE3C, LCE3D, LCE3E, LCE2C and LCE2D. The method for detecting psoriasis susceptible population by the region is realized by the following steps: extracting genome DNA; amplifying PCR; purifying by SAP alkaline phosphatase; allowing single base extension reaction of SNP sites according to a UEP primer designed by the SNP sites in the region; scanning by a mass spectrometer, judging difference of the SNP sites in the susceptibility gene region according to different values of molecular weights of various single base extension products; and judging results. The invention discloses the susceptibility gene region closely related to psoriasis, and provides the method for accurately and effectively determining the psoriasis susceptible population according to hereditary properties of the susceptibility gene region.

The invention discloses a molecular biological method for identifying goose variety. Based on the known research reports, polymorphic abundant microsatellite primers are screened, the individual of different goose varieties is scanned by an ABI 3730XLDNA full-automatic gene analyzer serving as a platform by combining the fluorescence-multiplex PCR technology and adopting the GeneScan-500 standard so as to obtain specific alleles of different varieties and construct a goose STR standard genetic map; and the variety authenticity of a sample to be detected is identified by comparing the STR map of the sample to be detected with a standard map. The method has the advantages of high yield, low cost, quickness, convenience and the like, is a reliable technical approach for scientifically evaluating and identifying the hereditary property of goose variety, and provides technical support for protecting goose breeding property and standardizing the breeding goose market.

The invention discloses development and application of a decaploid agropyron elongatum tandem repeat sequence specific probe. According to the development and the application, a specific length amplified fragment sequencing technology (SLAF-seq) is utilized to perform sequencing on common wheat, a common wheat-decaploid agropyron elongatum translocation line and decaploid agropyron elongatum, a decaploid agropyron elongatum specific sequence is acquired through sequence data comparative analysis, and the tandem repeat sequence specific probe is developed based on the decaploid agropyron elongatum specific sequence. The tandem repeat sequence specific probe provided by the invention can be used for rapidly identifying decaploid agropyron elongatum chromosomes and identifying a translocation line in a process that chromosome fragments of agropyron elongatum are transferred into the wheat, providing an important way of molecular marker-assisted selection to improve the wheat hereditary property and also providing an important application basis for wheat molecular breeding, phyletic evolution and germplasm resource identification.

The invention relates to the field of a plant planting technology and particularly relates to a method for artificially cultivating resveratrol-enhanced polygonum cuspidatum through tissue culture. The method comprises the following steps of (1) selecting and neatening a cultivating land; (2) performing ex vitro rooting of polygonum cuspidatum tissue culture seedling; (3) field planting; (4) performing field management; (5) controlling pests; (6) harvesting and processing. Polygonum cuspidatum seedlings mainly prepared by a plant tissue culture technique are regular, excellent in quality, and steady in hereditary property. According to the ex vitro rooting of the tissue culture seedling, the seedling raising period is shortened and the product cost is reduced; in the entire process, pesticides and heavy metals are nearly absent, thereby effectively ensuring the quality of polygonum cuspidatum; for the polygonum cuspidatum planted by the method, the effective active ingredients include 0.464-0.532% of resveratrol and 2.651%-2.853% of polydatin and are obviously increased when being compared with those of the wild polygonum cuspidatum; in comparison with an ordinary artificial cultivation method, the acre yield reaches 2000kg is increased.

The invention discloses a strain of penicillium expansum F3 and the application of the penicillium expansum F3 in preparing oligo-galactose. The preservation number of the strain is CGMCC No. 2352, a bacterial colony thereof grows rapidly with radiation rills and olive green color, the strain is light pink in the late growth stage, a conidiophore is smooth, broom-shaped branches are not symmetrical, a conidia is burn on a little peduncle with a chain shape, and the conidia takes an ellipsoid shape. Immobilized enzyme which is obtained through taking freeze-thaw whole cells of the strain as an enzyme source and adopting an alginate calcium embedding method is very stable and can be repeatedly used to synthesize the oligo-galactose, and the immobilized enzyme reacts for 11 batches and keeps the output more than 20%. The strain of the invention can be simply cultured, the hereditary property is stable, the method for preparing the immobilized enzyme is convenient, the property of enzyme is stable, the advantages for applying method in industrial production of the oligo-galactose are prominent, and the prospect is optimistic.

The invention discloses a zelkova serrata grafting reproduction method, which comprises the following steps that high-quality lignified or semi-lignified branches on clonal mother trees are adopted as scions, the length of the scions is 8 to 10cm, the scions have two to three buds, and then, the scions are cut into wedge-shaped scions with the length being 3.5 to 4.5cm; proper stocks are adopted, an opening is cut in the center, the cut scions are fast inserted into the opening, after the scion insertion, the contact parts of the scions and the stocks are wrapped out by yellow mud, the buds are avoided during the wrapping, and then, the cut opening parts are sealed through film coating or wax coating. According to the zelkova serrata grafting reproduction method, the zelkova serrata branches or the buds with the good property expression are grafted onto the stocks so that the stocks are heeled and survived into an independent plant. The root systems of the grafting seedlings have the hereditary properties of the stock plants, the trunks and the crowns are the continuation of the growth and the development of the scion stock plants, the characteristics of the stock plants can be maintained, and the hereditary is stable. Through the grafting reproduction technology, good characteristics of good seeds are maintained, the survival rate is improved, and the defects of great scion consumption and long seedling forming time of the grafting reproduction are overcome.

The invention relates to an azolla germplasm resource preservation device, which comprises a base and a cultivation layer frame, wherein preservation boxes for persevering azolla germplasm resources are placed on the cultivation layer frame; a lamp plate component is arranged above the various preservation boxes; water inlet hoses and water outlet hoses all of the various preservation boxes are mutually connected in parallel onto water inlet branch pipes and a return water branch pipe respectively; the water inlet branch pipes and the return water branch pipe are mutually connected in parallel onto a water inlet main pipe and a return water main pipe respectively; a liquid storage tank is arranged in the base, and is connected with the water inlet main pipe and the return water main pipe respectively to form a preservative fluid closed loop; an PLC (Programmable Logic Controller) is arranged in the base, the input end of the PLC is connected with a sensor monitoring unit, the output end of the PLC is connected with the preservative fluid closed loop and an environment control unit to form a closed loop control system, and the PLC is also connected with a GPRS (General Packet Radio Service) communication module. The device has the advantages that independent preservation is realized to prevent the mixing of the azolla resources and the cross contamination of pest and disease damage, a preservation quantity of germplasm resources can be flexibly adjusted, the functions of growth and integrated environment monitoring, adjustment and control are achieved, and the excellent character and hereditary property of the azolla varieties can be preserved permanently.

The invention relates to anthurium SSR (Simple Sequence Repeat) primer pairs and a kit based on transcriptome sequencing development. The invention provides sequences of 22 anthurium SSR primer pairs and a kit for identifying genetic relationship and hereditary properties of anthurium. The 22 primer pairs have polymorphism in 50 anthurium species, and match the hereditary diversity analysis on the 50 anthurium species with common knowledge of plant classification. The 22 primer pairs can be directly applied to more anthurium plant species to perform hereditary diversity analysis on germ plasm resources.

The invention relates to an excellent microorganism strain for space mutation, in particular to a good microorganism strain for space mutation, and the preparation method and the application thereof. The discoveries that the bump of a cancer patient infected by hemolytic streptococcus can subsidise naturally and the hemolytic streptococcus and the preparation thereof have a certain of biological activities are made is as early as in the 19th century. In the invention, the special environment of the outer space is utilized to deliver the strains to the outer space through carrying the 22nd recoverable experimental science satellite, the 22nd recoverable experimental science satellite safely returns after traveling for a certain period of time, the suppresormutation strains are eliminated, the forward mutation strains with excellent features are selected out for re-screening, and ultimately the excellent microorganism strains with vigorous life, quick growth speed, high fermentation yield, short fermentation period, high polypeptide content, stable hereditary property, strong productive adaptability and space cumulative effect. The microorganism strain can be applied to the strains for industrialization production, and has the advantages of high output, wide application range, low cost and high effective component content.

The invention discloses bacillus amyloliquefaciens capable of producing glutaminase and the application thereof and belongs to the field of microorganism screening and fermentation.The strain bacillus amyloliquefaciens BOSAR-X6 is preserved in the China General Microbiological Culture Collection Center on November 30, 2015 with the preservation number of CGMCC No.11760.The culture and fermentation conditions of the strain are simple, hereditary property is stable, the enzyme generated through fermentation has glutamine activity, liquid fermentation efficiency is high, operation is easy, and industrial production can be achieved more easily.Due to the fact that the strain comes from high-temperature hot springs, secreted glutaminase has special characters of salt resistance and high temperature resistance and has important industrial utilization value.

The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seedstorage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-teacamellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

The invention discloses a method for realizing in-vitro preservation of cattleya germplasm resources and recovering the growth after the preservation. The method comprises the following steps: stripping a stem tip from a new growing pseudobulb, putting the stem tip on a surface of a protocorm induction culture medium and culturing to obtain a protocorm; carrying out repeated cutting and subculture on the protocorm, so as to reproduce the protocorms with the needed quantity; culturing by adopting an in-vitro preservation culture medium; after the protocorms enter a development and growth phase, culturing in a dark place at 0 to 5 DEG C; transferring the protocorms which are cultured in the dark place into a differentiation culture medium; differentiating the protocorms to generate seedlings. According to the method disclosed by the invention, a cattleya stem tip culture technology is utilized to induce the protocorms and the protocorms are differentiated to obtain the cattleya seedlings. The protocorms are preserved at low temperature and a proper amount of paclobutrazol is added, so that the preservation time can be greatly prolonged and the number of times of subculture is reduced; the hereditary properties of the germplasm resources are effectively kept. After the protocorms are preserved for certain time, the protocorms can be cultured at normal temprature and under illumination conditions, and the growth of the protocorms can be rapidly recovered and the seedlings are differentiated.

The invention relates to an excellent microorganism strain for space mutation, in particular to a good microorganism strain for space mutation, and the preparation method and the application thereof. The discoveries that the bump of the cancer patient infected by hemolytic streptococcus can subsidise naturally and the hemolytic streptococcus and the preparation thereof have a certain of biological activities are made is as early as in the 19th century. The invention utilizes the special environment of the outer space to deliver the strains to the outer space through carrying the 20th recoverable experimental science satellite, the 20th recoverable experimental science satellite safely returns after traveling for a certain period of time, the suppresormutation strains are eliminated, the forward mutation strains with excellent features are selected out for re-screening, and ultimately the excellent microorganism strains with vigorous life, quick growth speed, high fermentation yield, short fermentation period, high polypeptide content, stable hereditary property, strong productive adaptability and space cumulative effect. The microorganism strain can be applied to the strains for industrialization production, and has the advantages of high output, wide application range, low cost and high effective component content.

The invention provides a chimeric virus RvHBJX of porcine reproductive and respiratory syndrome and an application of the chimeric virus and belongs to the technical field of genetic engineering in virus. The chimeric virus RvHBJX of porcine reproductive and respiratory syndrome provided by the invention is a chimeric virus which comprises a JXwn06 structural protein coding region of a high pathogenicity PRRSV strain by taking a low pathogenicity PRRSV strain HB-1 / 3.9 as a framework. After the chimeric virus is continuously passed for 80 times in vitro through an MARC-145 cell, the hereditary property of the virus is stable. The adaptability of the virus on a host cell is gradually enhanced along increase of the passage numbers. After the chimeric virus is passed for 60 times (P60) or over 60 times, the animal body is relatively good in safety, and the inoculated virus can provide 100% immune protection for high pathogenicity PRRSV strain and can be used as a vaccine candidate for the porcine reproductive and respiratory syndrome. The chimeric virus for preventing and treating porcine reproductive and respiratory syndrome has a good application prospect.

The invention provides a chimeric virus RvHBJX of porcine reproductive and respiratory syndrome and an application of the chimeric virus and belongs to the technical field of genetic engineering in virus. The chimeric virus RvHBJX of porcine reproductive and respiratory syndrome provided by the invention is a chimeric virus which comprises a JXwn06 structural protein coding region of a high pathogenicity PRRSV strain by taking a low pathogenicity PRRSV strain HB-1 / 3.9 as a framework. After the chimeric virus is continuously passed for 80 times in vitro through an MARC-145 cell, the hereditary property of the virus is stable. The adaptability of the virus on a host cell is gradually enhanced along increase of the passage numbers. After the chimeric virus is passed for 60 times (P60) or over 60 times, the animal body is relatively good in safety, and the inoculated virus can provide 100% immune protection for high pathogenicity PRRSV strain and can be used as a vaccine candidate for the porcine reproductive and respiratory syndrome. The chimeric virus for preventing and treating porcine reproductive and respiratory syndrome has a good application prospect.

The invention relates to anthurium SSR (Simple Sequence Repeat) primer pairs and a kit based on transcriptome sequencing development. The invention provides sequences of 22 anthurium SSR primer pairs and a kit for identifying genetic relationship and hereditary properties of anthurium. The 22 primer pairs have polymorphism in 50 anthurium species, and match the hereditary diversity analysis on the 50 anthurium species with common knowledge of plant classification. The 22 primer pairs can be directly applied to more anthurium plant species to perform hereditary diversity analysis on germ plasm resources.

The invention relates to a breeding method of a common mudflat shellfish with stress resistance and high yield, particularly a method of adopting second instar wild type mudflat shellfish as parent to establish a sib family and selecting one family with fastest growth, highest survival rate and highest rate from the first filial generation. By choosing excellent ones for breeding from the first filial generation, the groups of two families with fastest growth and highest survival rate are carried out cross-breeding, the fine strain with highest yield is chosen which is carried out the above breeding, and finally the fine variety with stress resistance and high yield is obtained. The advantage of the method is that the genes with economic character in the family can be purely combined fast, thus obtaining a new variety with high yield, stable yield and stress resistance. The family material can be used for study the hereditary property of mudflat shellfish so as to construct the gene linkage map of the mudflat shellfish.

The invention discloses a rejuvenation technology of agricultural microorganism strains by using chlorine dioxide, which comprises the following steps: firstly, microscopic examination is conducted on a shaking flask culture solution of bacteria to be rejuvenated; when in logarithmic growth phase, adding CLO2 solution so as to cause the concentration of CLO2 in the whole culture solution to reach 40 to 120ppm and diluting with aseptic water after shaking treatment are conducted; bacteria suspension containing remained vigorous thalli is coated on a flat plate for culturing and the remained vigorous thalli is examined and enters a culture medium inclined surface until fully distributing the inclined surface; the specific function detection of the strains is conducted and the strains to be improved in respect of specific function to an expected value enter the culture medium inclined surface for culturing and are put in a fridge for later use. The rejuvenation technology adopts chlorine dioxide as a declining individual eliminating agent, is characterized by being safe, changing no hereditary property of the strains, leaving no potential risk and quite obvious restorative improvement on specific function, and has large screening quantity, short and less working procedures, high effectiveness and quite good operational performance.

Disclosed is a species specificity primer used for amplifying Scutellospora calospora, which relates to a specific primer used for amplifying the Scutellospora calospora. The primer solves the problem that the existing primer can not directly amplify the sequence at the 25S rDNA D1 / D2 variable area part having hereditary properties of Scutellospora calospora from a mixed DNA sample. The species specificity primer for amplifying the Scutellospora calospora is 5`-TTAAAGCCATTACGTCAGCG-3`. The matching of the primer of the invention and an LR1 primer in a general matched primer can accurately amplify target fragments of Scutellospora calospora.

A species-specific primer pair for detecting glomus versiforme relates to a primer pair for detecting glomus versiforme, and is used for solving the problems that the existing primer pair cannot accurately and directly detect the partial sequence of a 25S rDNA (recombinant deoxyribonucleic acid) D1 / D2 variable region with the glomus versiforme hereditary property in a sample which contains active microorganism (AM) fungi, and the existing primer pair LR1 / G.ver cannot specifically amplify the corresponding specific DNA fragment from a hybrid sample. The species-specific primer pair for detecting glomus versiforme is named as G.ver F / G.ver R, and is composed by a forward primer G.ver F and a reverse primer G.ver R. The species-specific primer pair is suitable for detecting the glomus versiforme.

The invention discloses a strain of penicillium expansum F3 and the application of the penicillium expansum F3 in preparing oligo-galactose. The preservation number of the strain is CGMCC No. 2352, abacterial colony thereof grows rapidly with radiation rills and olive green color, the strain is light pink in the late growth stage, a conidiophore is smooth, broom-shaped branches are not symmetrical, a conidia is burn on a little peduncle with a chain shape, and the conidia takes an ellipsoid shape. Immobilized enzyme which is obtained through taking freeze-thaw whole cells of the strain as anenzyme source and adopting an alginate calcium embedding method is very stable and can be repeatedly used to synthesize the oligo-galactose, and the immobilized enzyme reacts for 11 batches and keepsthe output more than 20%. The strain of the invention can be simply cultured, the hereditary property is stable, the method for preparing the immobilized enzyme is convenient, the property of enzyme is stable, the advantages for applying method in industrial production of the oligo-galactose are prominent, and the prospect is optimistic.

The invention relates to an aspergillus strain, in particular to the aspergillus oryzae for efficiently degrading nicotine and a culturing method and application thereof, and belongs to the field of microbiological technology. The aspergillus oryzae 112822 strain has been preserved in the normal microorganism centre of China Committee for Culture Collection of Microorganisms on march 29, 2010 with a preservation number as CGMCC No. 3687. The strain has the advantages of simple culturing, stable hereditary property, the capability of degrading the nicotine in the tobacco leach liquor to be 7.8g / L when applied to the degradation of the nicotine and having a broad industrial application prospect.

The invention discloses a tissue culture method of green chrysanthemums. The tissue culture method includes explant sterilization, induced culture of calluses, bud differentiation and subculture enrichment culture, rooting and seedling hardening culture, and acclimatization and transplant. The tissue culture method has the advantages that culture time for green chrysanthemum seedlings is short, the obtained green chrysanthemum seedlings have few viruses and stable hereditary properties, the obtained green chrysanthemum branches are thick, wind resistance is high, and the green chrysanthemums have stronger vitality and higher survival rate than green chrysanthemums subjected to tissue culture in the prior art; active oligopeptides with the amino acid sequences of SCASVCKAHCYCRCGSVFHRGCRCLRC are added into a bud differential medium, and can induce the calluses to re-differentiate, regulate dormancy process, break axillary bud dormancy and shorten budding time, and accordingly, budding efficiency is high; multiplication speed of differentiated cells can be increased obviously, the quantity of multiple shoots is high, and the obtained multiple shoots have large and green leaves and strong vitality.