Alternaria radicina bacterial strain for producing alpha-L-rhamnoside enzyme and cultivating method as well as application thereof

A technology of rhamnosidase and Alternaria, which is applied to Alternaria strains and its cultivation method and its application in the hydrolysis of naringin, and can solve the problems of limited species

Active Publication Date: 2012-01-04
SHANDONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the limited species of fungal microorganisms producing α-L-rhamnosidase in the industrial application of existing microbial enzymatic methods, especially the limited species of microorganisms that produce α-L-rhamnosidase with strong hydrolytic activity and good stability , the object of the present invention is to provide a new isolated Alternaria strain that can produce strong hydrolytic activity and good stability of α-L-rhamnosidase, the α-L-rhamnosidase produced by this bacteria can be Efficiently catalyzes the hydrolysis of naringin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alternaria radicina bacterial strain for producing alpha-L-rhamnoside enzyme and cultivating method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] Alternaria sp.L1 strain producing α-L-rhamnosidase, the strain was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection on March 25, 2010, and the preservation number is CGMCC No.3688 .

[0007] Alternaria L1 strain grows rapidly on the potato medium, and the colony spreads in 2 to 3 days, producing a large amount of mycelium. The colony is white at first, and turns greenish brown in about 3 days, and gradually turns brown to dark with the extension of culture time. Brown, the hyphae in the base produce a water-soluble brown pigment. The conidiophores are born on the side and top of the aerial hyphae, and the separation is constricted. The conidia are solitary or in chains on the conidiophores, which are inverted pear-shaped or ovoid, with horizontal, vertical, Oblique septum, separated in a brick lattice, with verrucous protrusions on the surface, brown or dark brown in color. The accession number of the 18S rDNA of this st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

Description

technical field [0001] The present invention relates to Alternaria strain and its culture method and application, particularly relate to a kind of Alternaria strain that can produce α-L-rhamnosidase (EC 3.2.1.40) and its culture method and its use in the hydrolysis of naringin application. technical background [0002] Naringin is the main bitter substance in the processing of citrus fruit juices. The removal of naringin is mainly divided into physical method and enzymatic method. The physical method has disadvantages such as poor controllability, affecting product quality and flavor, and complicated process, while the enzymatic method has attracted more and more attention due to its advantages of simple operation, easy control, and better retention of product nutrition and flavor. α-L-rhamnosidase can hydrolyze naringin, and the bitterness of the hydrolyzed product is much smaller than that of naringin, thus improving the taste of fruit juice. [0003] Most of the reporte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/14C12R1/645
Inventor 肖敏刘倩卢丽丽
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap