The invention relates to a preparation method for eggs rich in omega-3 polyunsaturated fatty acids. The preparation method comprises the following steps of: (1) adding screened rubber seed kernel or rubber seed oil cake into non-gm feed for laying hens, with the additive amount of the rubber seed kernel being 10-20% of the total weight of the feed for laying hens, and the additive amount of the rubber seed oil cake being 15-25% of the total weight of the feed for laying hens; (2) adjusting ingredients of the feed to enable the feed to comprise the following ingredients: 42.89 of corn, 17-32 of soybean meal, 0.59 of wheat bran, 2-3 of lard, 10-20 of rubber seed kernel or 15-25 of rubber seed oil cake, 0.35 of salt, 8.38 of stone powder, 1.8 of calcium hydrogen phosphate, 0.1 of lysine, 0.14 of methionine, 1 of premix for laying hens, and 5 international units of vitamin E per kg of feed; (3) feeding 130-140-day laying hens by using the feed for laying eggs rich in omega-3 polyunsaturated fatty acids after feeding for 2-4 weeks. The preparation method is low in cost, stable in content of the omega-3 polyunsaturated fatty acids, and safe without peculiar smell, and effectively and sufficiently utilizes rubber seed resources.