29 results about "Liana" patented technology

A system for forming trunks of trees, plants, and vines into predetermined letter, word, numerical, and shape configurations having a wire assembly with a guide wire having a predetermined configuration; and a trunk of a predetermined thickness, whereby the trunk is secured to the guide wire, wherein the trunk adopts the configuration of the guide wire as it grows, thickens, and matures. The guide wire is a single continuous wire and is of a predetermined gauge to be shaped into said predetermined configuration. The configuration is a letter, letters, a word, words, a number, numbers, and / or a geometric shape, shapes. The trunk is secured to said guide wire with zip ties that are positioned separated a predetermined distance from each other allowing the trunk to align with the guide wire to adopt to the predetermined configuration.

This invention relates to the field of cultivation technology for specific crops or plants not mentioned above, and discloses a method for creating vegetation on alluvial and rockfall riverbanks. The method involves identifying weak erosion zones less affected by unstable landslides and alluvial deposits left by flash floods through exploration. Perennial vines are then planted at these locations to cover the remaining riverbank area, preventing damage to the vegetation from alluvial deposits and fallen rocks. Microenvironmental wells are dug within the weak erosion zones, and the perennial vines are planted inside to block flash floods from damaging the vegetation and surrounding soil. The soil within the wells is rotated, with topsoil containing a large amount of sand and gravel, being infertile and unable to retain water, being turned to the bottom of the well, thus no longer hindering vegetation growth and facilitating groundwater infiltration. The deeper soil, free of sand and gravel, relatively fertile, and with better water retention, is turned to the top layer, allowing the vegetation within the well to grow better. The combination of these methods overcomes the damage and stress to vegetation, enabling the creation of vegetation on riverbanks where normal growth is impossible.

A system and a method for protecting a single-row planting from insects. The plants may be trees, shrubs or vines. The system comprises a row of posts aligned with the single-row of plants, a shield extending over a length of the row of posts, a tray below branches of the plants and two end covers. The bottom tray, which extends along the length of the row of plants, has apertures to accommodate and snugly seal against the posts and the stems of the plants. In a deployed position, the shield is operable to engage the bottom tray to seal against it. The two end covers seal against the shield and against the tray, thereby protecting the branches of the plants from insects. The shield may use netting material.

The present invention relates to fungicidal combinations and compositions comprising two or more fungicidal compounds for controlling fungal diseases in plants. In particular, the present invention provides bactericidal products for controlling fungal diseases of woody liana such as grapes. The invention also relates to a corresponding method and to the use thereof.

The invention particularly relates to a seawall slope protection ecological reconstruction structure and a construction and maintenance method thereof.The seawall slope protection ecological reconstruction structure comprises a dam body, transverse concrete retaining walls distributed in a stepped mode are arranged on the dam body, and a plurality of planting pit grooves are formed in the dam body in combination with longitudinal concrete retaining walls; the planting pit slots can be rapidly filled with organic fertilizer soil through mixed feeding equipment so as to efficiently achieve the purpose of planting plants, the slope of the whole dam body is slowed down in a stepped mode after soil filling, and under the action of the retaining walls, water and soil loss can be effectively prevented, the survival rate of vegetation can be effectively increased, and the service life of the dam is prolonged. Planting pits below the high tide line of the dam body are close to the sea and used for planting plants with high salt tolerance, such as cypress, mirabilis, suaeda glauca and ipomoea pes-caprae, and shrubs and grasses (vines) plants with high drought resistance, such as vitex rotundifolia, zoysia japonica, oleander, ruellia delavayi and agave angustifolia, are planted in the planting pits when the middle-upper part of the dam body is far away from the sea. Sustainable development of the seawall is achieved, and the ecological function of the seawall is improved.

The utility model discloses a soilless culture device, which relates to the technical field of soilless culture and comprises a culture frame, culture pipes arranged on the culture frame and a liquid storage tank. A top ring used for supporting the liquid storage tank is arranged at the top of the cultivation frame, and an outer ring used for supporting the cultivation pipes is arranged on the outer circle of the cultivation frame; the cultivation pipes are vertically arranged, a plurality of seedling raising openings are formed in the upper surfaces of the cultivation pipes, liquid conveying pipes are arranged on inner rings of the cultivation pipes, the upper ends of the liquid conveying pipes are communicated with the liquid storage tank through hoses, and one sides of the liquid conveying pipes are communicated with the cultivation pipes through branch pipes; and a ball valve for controlling the pipeline to be opened and closed is arranged on the branch pipe. According to the utility model, the plurality of cultivation pipes arranged up and down are placed on the cultivation frame, so that vines can climb and grow along the cultivation frame more conveniently; by means of the annular cultivation pipes arranged on the upper layer and the lower layer, the occupied space is reduced, and meanwhile the cultivation number of plants is increased.

The utility model discloses a directional breeding device. The directional breeding device comprises a first plate body, when the device is used for breeding and culturing plants, if the bred plants are vine plants or tall plants, objects such as bamboo poles are usually needed to enable the plants to climb or be bound manually, so that the telescopic rod head is driven by the electric telescopic rod to drive the second plate body, the movable plate, the second frame body and the like to move synchronously, and the plants can be bred and cultured through the second plate body, the movable plate, the second frame body and the like. The planting table can be synchronously expanded outwards, so that the second rod bodies are pulled out of the inserting grooves, the first rod bodies and the second rod bodies can be popped out through springs installed on the first rod bodies, a bamboo pole frame structure is formed, plants can be supported, vine plants can climb or high-seedling plants can be bound, and the planting table is convenient to use and high in practicability. Meanwhile, sunlight irradiation can be fully received for photosynthesis, development and maturity of the fruits are facilitated, and therefore it is guaranteed that the breeding effect is more ideal.

This invention relates to the field of climbing attachment claw technology, specifically to a vine-like biomimetic self-growing claw, comprising a first gripping part and a second gripping part arranged in opposition, a shell, and a drive motor, a first spiral shell, a first winding shaft, a first torsion spring, a first double-layer gear, an incomplete gear, a first energy storage gear, a second spiral shell, a second winding shaft, a second torsion spring, a second double-layer gear, and a second energy storage gear disposed therein. The first gripping part and the second gripping part are both elastic coil springs, each wound around the first winding shaft and the second winding shaft, which are respectively fixed to the center inside the first spiral shell and the second spiral shell. This invention has a simple structure and a relatively small size, making it easy to carry and install, and can easily achieve variable diameter movement with good adaptability.

The utility model relates to the field of buildings, and discloses an iron pole vine tree climbing device which comprises a base table, an iron frame body and a planting box body. The iron stand main body is fixedly arranged at the top of the base table, and the multiple planting boxes are arranged on the circumference of the top of the base table around the axis of the iron stand main body; the iron stand main body is detachably provided with a spraying assembly, and the spraying assembly is used for spraying drip irrigation on the planting box body; the planting box bodies are arranged at the top of the base platform around the iron stand main body, and meanwhile, the spraying assembly is detachably and fixedly arranged on the iron stand, so that the trouble and inconvenience caused by direct hole planting of vines on the ground are avoided through the arrangement of the planting box bodies; the planting box body can more conveniently adjust and limit the planting position of the liana, a more stable and reliable growth environment is provided for the liana, the spraying assembly can simulate the rainwater dripping environment to provide water for the liana, growth of the liana can be promoted and guided, and the growth efficiency of the liana is improved.

A system for forming trunks of trees, plants, and vines into predetermined letter, word, numerical, and shape configurations having a wire assembly (20) with a guide wire (22) having a predetermined configuration; and a trunk (42) of a predetermined thickness, whereby the trunk (42) is secured to the guide wire (22), wherein the trunk (42) adopts the configuration of the guide wire (22) as it grows, thickens, and matures. The guide wire (22) is a single continuous wire and is of a predetermined gauge to be shaped into said predetermined configuration. The configuration is a letter, letters, a word, words, a number, numbers, and / or a geometric shape, shapes. The trunk (42) is secured to said guide wire (22) with zip ties (24) that are positioned separated a predetermined distance from each other allowing the trunk (42) to align with the guide wire (22) to adopt to the predetermined configuration.

The utility model discloses an economic liana slope protection device based on bamboo tube hole planting, including planting module, the planting module includes a plurality of bamboo tube main part, and the bamboo joint of two is remained on every bamboo tube main part, wherein the bamboo joint top of the position of being located in the upper is equipped with planting hole, and the planting hole is used for embedding the seedling of liana, and the side wall of bamboo tube main part is annularly provided with a plurality of drainage holes on the upper position of the bottom bamboo joint, and the deeply buried bamboo tube itself constitutes micro "pile foundation", can effectively resist the slip of shallow soil body, root system guide and strengthen, and the strong root system of bamboo tube guides the strong root system of yam, kudzu root and other plants vertically downward and deeply digs to stable soil layer, forms "root pile", and the anchoring effect is remarkable, and far more than shallow root plant. The vine covers the slope surface quickly, and reduces the direct scouring of rainwater, initial protection: bamboo tube provides the microenvironment of windbreak, soil conservation, anti -scouring for seedling, improves survival rate and early growth speed, and shortens the effect time of slope protection.

The invention discloses a tree species and vine plant composite cultivation method which comprises the following steps: (1) investigating and surveying a karst landform area, taking the karst landform area as a planting area, selecting and planting specific tree species in the karst area, and selecting and planting sun-resistant vine plants; (2) digging holes in the planting area to plant tree seedlings; (3) building a pergola around the tree species, wherein the height and the width of the pergola are both greater than the increase of the plant height and the crown breadth of the tree species; (4) planting vine medicinal materials along the periphery of the bottom of the pergola, and guiding vines to grow around the pergola; and (5) removing the vine climbing frame after planting for 1-2 years, and erecting vines of the vine plants on trunks of the tree species. According to the cultivation method, the stress resistance of the rare tree seedlings is greatly improved, the rare tree seedlings can adapt to the climatic environment of the area, the plant height and the diameter are obviously increased, and at the moment, the rare tree plants can provide good climbing conditions for liana plants and help the liana plants to increase the growth amount.

The invention relates to a triterpenoids type compound as well as a preparation method and application of the triterpenoids type compound. The compounds 1 to 5 provided by the invention are extracted and separated from a vine shrub plant Tripterygium wilfordii Hook.f. Of a Euonyriaceae tripterygium wilfordii, wherein the compounds 3 and 4 are new compounds of triterpene derivatives with novel structures, and the compounds 1 to 5 provided by the invention are used for extracting and separating the compounds 1 to 5 from a vine shrub plant Tripterygium wilfordii Hook.f. Of the Euonyriaceae tripterygium wilfordii Hook.f. Bioactivity experiments show that the obtained triterpenoid compound has ATP citrate lyase (ACLY) inhibitory activity and can be applied to research and development of drugs for various cancers, hyperlipidemia, atherosclerotic cardiovascular diseases and the like.

The utility model discloses a parapet wall with a greening climbing frame, which relates to the technical field of parapet walls, and comprises a wall body, the climbing frame is fixedly mounted on the top surface of the wall body, a water storage tank is fixedly mounted on the surface of the wall body, a grating is arranged in the wall body, a filter plate is inserted in the water storage tank, and the greening climbing frame is fixedly mounted on the wall body. A drainage hole A is formed in the surface of the wall in a penetrating mode, a planting groove is formed in the wall, a through hole is formed in the connecting position of the wall and the water tank in a penetrating mode, a drainage hole B is formed in the side face of the water storage tank, a limiting groove is formed in the surface of the water storage tank, and a sliding rod is slidably connected into the water storage tank. According to the parapet wall, liana plants are planted in the planting grooves, the liana plants can grow upwards along the climbing frame, the parapet wall where the plants are planted can absorb carbon dioxide and release oxygen through the photosynthesis of the plants, dust and harmful gas can be further adsorbed, and the air quality is improved.

The invention relates to the technical field of highway engineering, and discloses a highway engineering slope greening carbon sink quantification method comprising the following steps: 1, vegetation investigation: S1, vegetation type identification: comprehensively identifying vegetation types in a highway engineering slope greening area, and covering arbors, shrubs, herbaceous plants and possible vines; according to the highway engineering slope greening carbon sink quantification method, through detailed vegetation investigation, soil data acquisition and environmental factor recording, rich basic data is provided for accurately quantifying the carbon sink capacity, the defect that data acquisition is incomplete in a traditional method is overcome, and the vegetation carbon sink is calculated by adopting a biomass method, so that the carbon sink quality is improved. The soil carbon sink is calculated in combination with the soil organic carbon content and volume weight, the soil carbon sink and the volume weight are integrated to obtain the total carbon sink, the calculation method is scientific and reliable, the carbon sink contribution of slope greening can be evaluated more accurately, and compared with a traditional single calculation method, the method has higher accuracy and credibility.

The utility model discloses a climbing structure for landscape plants, and relates to the technical field of landscape greening. The planting device comprises an upper building reverse ridge and a lower building reverse ridge which are horizontally arranged and distributed up and down, a planting box is arranged on the surface of the lower building reverse ridge, climbing assemblies are evenly arranged in front of the planting box in the length direction of the planting box, and each climbing assembly comprises an inhaul cable perpendicular to the upper building reverse ridge and the lower building reverse ridge. An upper hook and a lower hook are correspondingly arranged above and below the inhaul cable respectively, an upper connecting ring and a lower connecting ring are fixed to the ends, close to the inhaul cable, of the upper hook and the lower hook, a connecting rod is fixed to the lower end of the lower connecting ring, a turn buckle is in threaded connection with the outer portion of the lower side of the connecting rod, and a lower hook is arranged at the lower end of the turn buckle. A climbing platform is provided for the vines through the climbing assembly, so that the vines climb more orderly, the ornamental effect is improved, and the problem that in the prior art, the vines climb along the surface of a building in a standard-free mode, and the ornamental effect of the landscape is reduced is solved.

The utility model relates to the technical field of garden decoration, in particular to a liana wall surface auxiliary climbing device which comprises a ground nail, a retaining assembly and a traction adjusting assembly, the ground nail is nailed on the ground close to a wall body, the upper end of the ground nail is vertically connected with a guide rope, the upper end of the guide rope is fixedly connected with a rivet, one side of the rivet is nailed on one side of the wall body, and the other side of the rivet is nailed on the other side of the wall body. The retaining assembly is slidably connected with the guide rope in a sleeving mode, the retaining assembly comprises a hinge sleeve and two clamping plates, the guide rope for guiding the liana is vertically arranged on one side of the wall through a ground nail and a rivet, and when the liana is fixed, the stem of the liana is clamped and fixed through the retaining assembly; then the top of the liana can be pulled through the pulling adjusting assembly, so that the liana can continuously and vertically grow in the growth process without increasing or adjusting a climbing fixing piece, in addition, the guide rope is arranged away from the wall and is matched with the clamping plate, the liana can be prevented from being in direct contact with the wall body, and the burning possibility is reduced.

The invention relates to a viticulture structure (1) comprising a post (A) provided with a post (A) through-hole (O1) through which a wire (A0) is drawn to support the transverse trunk of a grape vine or liana, in addition, said post (A) comprising a temporary arm (B1) or a fixed arm (B), the fixed arm (B) being configured optionally in the form of a straight arm or in the form of a bent mid-section or in the form of a split mid-section, the post (A) being provided with a post (A) through-hole (O1) through which a wire (A0) is drawn in order to support the transverse trunk of the grape vine or liana. The bending angle is 0-80 degrees, and two inclined supporting rods (C) are selected and matched; and a cultivation system comprising several grape cultivation structures (1) comprising several struts (A), each of which has a strut (A) through-hole (O1) through which a wire (A0) is drawn to support the transverse trunk of a grape vine or liana, in addition, said struts (A) comprising a temporary arm (B1) or fixed arm (B) and optionally two diagonal struts (C).

The invention relates to a high fill composite structure retaining wall, which relates to the technical field of ecological protection, and comprises a high fill soil body, a wall body main body is fixed on the left side of the high fill soil body, a vegetation mechanism is arranged on the wall body main body, and an inverted filter drainage layer used for preventing fine particle loss and guiding water is fixed between the high fill soil body and the wall body main body. According to the high fill combined structure retaining wall, the inclined planting holes penetrating through the wall body main body, the inverted filter drainage layer and the high fill soil body are formed, and the root system suction layer, the improved soil dressing and the water retention type planting matrix are sequentially arranged in the holes, so that plant root systems are effectively guided to actively penetrate through the rigid wall body to enter the deep fill soil body, and the living body reinforcement effect is formed; compared with a traditional vegetation bag or a spray-seeding layer only attached to the surface, the structure enables root systems of herbaceous plants, shrubs and vines to be interwoven in a filling body in a three-dimensional mode, the shear strength and the overall stability of a soil body are remarkably improved, and the problems that an existing ecological protection face is prone to degradation and short in function are thoroughly solved.

The utility model relates to the technical field of light supplementing lamp stand, concretely is a light supplementing lamp stand with adjustable light and illumination angle for liana, the utility model discloses the base bottom equidistance fixedly connected with four groups of universal wheel, through the light supplementing lamp drive slider up and down sliding in the sliding slot, and drive the connecting frame to move synchronously, until the light supplementing lamp moves to the proper position, the plug rod is aligned with any one set of limit hole at this moment, again through the restoring force of spring is pushed the fixed disc, makes the fixed disc drive plug rod inserts into the limit hole, thereby reaches the height adjustment purpose to single set light supplementing lamp, can avoid surrounding wall body, furniture or other obstacles, makes the adjustment operation smoothly displays, ensures that light supplementing lamp can reach ideal height position, makes full use of the limited indoor space, and simultaneously aims at the dense and irregular growth form of liana vine, avoids the collision of multiple light supplementing lamps when lifting simultaneously with the vine.

The utility model relates to the technical field of turnover box, concretely is a kind of gourd family liana seedling transportation turnover box with moisturizing function, including box, side door is installed in box one side, multiple storage components are installed in box, vertical placing groove is opened in the inner wall of box, moisturizing layer is installed in placing groove, water storage tank that box bottom end is provided with with the bottom end contact of moisturizing layer, humidity sensor is arranged in the inside of box top end, control module is arranged in the outside of box, the box is provided with spraying humidification component, the utility model provides moisture basis for moisturizing layer by water storage tank, moisturizing layer maintains the basic humidity in box by its own characteristics, humidity sensor monitors the temperature and humidity in box in real time and data transmission to control module, when humidity is lower than set value, control module starts spraying humidification component to supplement humidity, realizes the purposes of automation, precision control of humidity in box.

The invention relates to the technical field of slope treatment and ecological restoration, and discloses a high and steep rock slope ecological restoration method which comprises the following steps: removing impurities and loose rock soil on the surface of a slope; a plant climbing net is hung, and the slope surface is reinforced; prefabricated bearing frames are transversely arranged along the slope surface at intervals of 1 meter, each prefabricated bearing frame is formed by welding two L-shaped main anchor rods and two U-shaped steel bar auxiliary supports with upward openings, and the supporting and protecting effects are achieved; ecological bags filled with composite matrix soil are laid on the upper portion of the prefabricated bearing frame; planting suitable shrub, grass and vine mixed plant seeds to form an ecological system taking vine plants as main plants and shrub herbs as auxiliary plants; and installing a drip irrigation system for vegetation maintenance. The ecological bags are fixed in a stepped mode through the prefabricated bearing frame, and the problem of abrupt slope base material sliding is solved; a vine-shrub-grass composite vegetation system is constructed, and the regreening effect is enhanced; a drip irrigation maintenance system is matched, and the vegetation survival rate is increased.

The invention relates to the technical field of indoor cultivation, and discloses an atomization cultivation machine which comprises a base located at the bottom, a planting box is arranged on the upper side of the base, a planting plate is arranged on the upper side of the planting box, a plurality of through holes for liana to grow are formed in the planting plate, a stand column is arranged on the side edge of the base, and the stand column is connected with the planting box. A top seat is arranged at the upper end of the stand column, and a connecting rope for liana to climb and wind is arranged between the top seat and the through hole. When the liana grows at the position of the through hole, the liana can climb and wind along the connecting rope between the top seat and the through hole and is supported by the connecting rope, so that the liana can grow on the planting box in the vertical direction, and finally the planting requirement of the liana can be met.

The application is a kind of sea embankment slope ecological reconstruction structure and its construction maintenance method, including embankment body, transverse concrete retaining wall with ladder distribution is arranged in the embankment body, combined with longitudinal concrete retaining wall, so that the embankment body forms a plurality of planting pits, the planting pit can be quickly filled with organic fertilizer soil by mixing feeding equipment, to efficiently realize the purpose of plant planting, and after filling, the whole embankment body slope is ladder type, and under the action of the retaining wall, it can effectively prevent water and soil loss and improve the survival rate of vegetation, the planting pit below the high tide line of the embankment body is closer to the sea, and it plants strong salt-tolerant plants such as sea spirogyra, bitter tree, alkali grass and thick vine, while the upper part of the embankment body is far from the sea, and the planting pit plants drought-resistant shrubs and grass (vine) plants such as single leaf vine, kikuyu grass, oleander, grey lily and narrow leaf agave, to realize the sustainable development of the sea embankment and improve the ecological function of the sea embankment.

The invention belongs to the technical field of plant cultivation, and provides a cultivation method for expanding plant growth and an aeroponic cultivation device, which comprises the following steps: inducing to grow new roots at the positions of branches of a plant far away from a trunk or the position of the trunk far away from the root, and providing nutrient substances required by the plant to the induced new roots. According to the method, new roots are induced to grow at the positions, far away from the trunk, of the branches of the plant or the positions, far away from the root, of the trunk, the limitation of atmospheric pressure and transpiration tension on plant growth can be broken through, and the purpose of prolonging growth of trees and perennial vines is achieved.

The utility model discloses a green planting structure of a stepped rubble side slope. The green planting structure comprises a water absorption blanket and a coconut shred blanket which are sequentially laid on the rubble side slope in a laminated mode. The multiple rows of supporting columns are sequentially arranged at intervals in the longitudinal direction of the rubble side slope, each row of supporting columns comprises a plurality of supporting columns arranged at intervals in the transverse direction of the rubble side slope, and the supporting columns are anchored to the rubble side slope from the upper surface of the coconut shred blanket; the climbing net rack is connected to the supporting columns and erected above the coconut shred blanket, and the climbing net rack is used for liana to climb; the first planting groove is formed in the bottom platform, and vines which like to climb upwards are planted in the first planting groove; the second planting groove is formed in the top platform, and vines which like to climb downwards are planted in the second planting groove. According to the scheme, the growth of the vines on the rubble slope can be promoted, and the planted vines adopt a mode of combining upper hanging and lower climbing, so that the rubble slope can be quickly regreened.

The utility model relates to the technical field of power tower anti-vine climbing, disclose a device of preventing vine climbing power tower. The utility model discloses: equipment shell and savings storehouse are connected with first equipment frame on equipment shell and are fixed, the top of first equipment frame is connected with servo motor, first equipment frame is rotatably connected with first gear in, first equipment frame is rotatably connected with second gear in, the output shaft of servo motor is fixedly connected with the rotating link on. The utility model can make the equipment when using can actively restrain the growth of vine, and start climbing through cutting, the through automatic spraying weeding water of growing makes can restrain vine in the initial stage, avoid the vine possibly through the secretion of mucus or growth lateral branch bypassing the anti-vine climbing device and grow, then the vine plant climbs the facilities of tower, causes the equipment short circuit, corrosion or structural damage etc. The problem occurs, and the safety of equipment when using is greatly improved.

The present application belongs to the technical field of slope greening, and particularly relates to a substrate for planting liana on high and steep slope and a preparation method thereof. A polybutylene succinate solution is mixed with peanut protein powder, and is sprayed on the surface of nutrient granules. The prepared coated slow-release granules are mixed with easily-absorbed small-molecule nutrient prepared by fermentation of a composite microbial agent, and the prepared fermentation-type slow-release nutrient agent can provide sufficient nutrients for liana in a persistent and efficient manner. After being mixed with methyl methacrylate anhydride gelatin, rough and hydrophobic modified conglomerate particles are added to the substrate, so as to prevent the substrate from sliding on the steep slope and promote the discharge of excess water. The base solution is mixed with the viscous substrate composition and the fermentation-type slow-release nutrient agent, and the prepared substrate for planting liana on high and steep slope is not easy to be lost, has strong water retention and drainage properties, and can provide nutrients for liana in a long-term and effective manner.

The invention relates to a preparation method of radix zanthoxyli protoplast, and belongs to the technical field of plant biology. The method aims to solve the technical problem that the whole protoplast is difficult to release by a conventional enzymolysis method due to high cell wall lignification degree and compact tissue structure when the radix zanthoxyli is used as a woody vine plant. According to the technical scheme, the method comprises the steps of culturing zanthoxylum nitidum seedlings; selecting terminal buds and tender leaves below the terminal buds, performing low-temperature dark pretreatment, and shredding; soaking the leaf filaments in an enzymatic hydrolysate which is based on a CPW solution and contains mannitol, cellulase R-10, pectinase Y-23 and macerozyme R-10, carrying out vacuum-pumping treatment, and carrying out constant-temperature oscillation enzymolysis; and after enzymolysis, the protoplast is obtained through the steps of filtration, centrifugation and resuspension purification. The prepared protoplast can be used for somatic cell hybridization, genetic transformation and single cell level research.