The invention relates to a long-distance transportation method of live
sepia esculenta. The method includes the following steps of selecting parent , temporary rearing before transportation, stopping feeding for 24 hours, and preparing water for transportation; according to the step of temporary rearing before transportation, temperatures of the water in a temporary rearing
pool is gradually transited to 16-18 DEG C, and the difference between the
salinity of the water in the temporary rearing
pool and the
salinity of cultivation water is controlled within 2; according to the step of preparing the water for transportation, water for temporary rearing is used as the water for transportation, the temperatures of the water for transportation are reduced to 12-14 DEG C,
pure oxygen is fed into the water for transportation, the live
sepia esculenta is taken out of the
pool, bagged and loaded in a foam box for transportation, the box is sealed and loaded to a vehicle, and temperature transition is performed when the live
sepia esculenta arrives at the destination. According to the method, defects that the sepia esculenta is prone to jet ink due to severe rocking of water in the long-distance transportation of the live sepia esculenta and extremely prone to die due to
high oxygen consumption rates and
oxygen deficit in transportation are overcome, costs for long-distance transportation of the live sepia esculenta are reasonable, the method is easy to master, the
survival rate of the long-distance transportation live sepia esculenta is over 95%, and the method has large market promotion values and can produce good economical benefits.