Pseudomonas aeruginosa for denitrification and phosphorous removal under different dissolved oxygen conditions and application of pseudomonas aeruginosa

A Pseudomonas aeruginosa, conditional technology, applied in the direction of bacteria, microbial-based methods, biological water/sewage treatment, etc., can solve the problems of nitrification and denitrification and phosphorus release and phosphorus absorption that cannot be organically unified, and achieve broad application prospects. The effect of shortening the process flow and saving infrastructure costs

Active Publication Date: 2013-01-16
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a strain of Pseudomonas aeruginosa that can perform denitrification and phosphorus removal under different dissolved oxygen conditions and its application, which solves the problems existing in the traditional nitrogen and phosphorus removal process. The problem that nitrification and denitrification and phosphorus release and absorption cannot be organically unified

Method used

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  • Pseudomonas aeruginosa for denitrification and phosphorous removal under different dissolved oxygen conditions and application of pseudomonas aeruginosa
  • Pseudomonas aeruginosa for denitrification and phosphorous removal under different dissolved oxygen conditions and application of pseudomonas aeruginosa
  • Pseudomonas aeruginosa for denitrification and phosphorous removal under different dissolved oxygen conditions and application of pseudomonas aeruginosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Denitrification and phosphorus removal ability determination of Pseudomonas aeruginosa under anaerobic conditions

[0038] The strain preserved in glycerol at -20°C (preserved in the General Microbiology Center of China Committee for Microorganism Culture Collection (CGMCC for short) on January 21, 2010, the preservation number is CGMCC No.3602, the same below). Inoculate in sterilized 100mL medium (each L contains 5.0g sodium succinate, 0.607g NaNO 3 , 0.110g K 2 HPO 4 ·3H 2 O, 0.10g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, 0.02g CaCl 2 2H 2 O, 0.10mL trace element mother solution (each L contains 3.5g EDTA, 2gZnSO 4 ·7H 2 O, 1g CuSO 4 ·5H 2 O, 1g MnSO 4 ·7H 2 O, 1 g Na 2 MoO 4 2H 2 O, 1g H 3 BO 3 , 0.2gCoCl 2 ·6H 2 O), pH 7.0~7.5) in a 250mL Erlenmeyer flask, placed in a 30°C, 150rpm shaker for 20~24h, so that the bacteria grew to the late logarithmic period, and the bacterial suspension was used for inoculation.

[0039] Take 5mL of ...

Embodiment 2

[0043] Embodiment 2: Nitrification and phosphorus removal ability determination of Pseudomonas aeruginosa under anoxic conditions

[0044] Inoculate the strains stored in glycerol at -20°C into 100 mL of sterilized medium (each L contains 5.0 g sodium succinate, 0.607 g NaNO 3 , 0.110g K 2 HPO 4 ·3H 2 O, 0.10g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, 0.02g CaCl 2 2H 2 O, 0.10mL trace element mother solution (each L contains 3.5g EDTA, 2g ZnSO 4 ·7H 2 O, 1gCuSO 4 ·5H 2 O, 1gMnSO 4 ·7H 2 O, 1gNa 2 MoO 4 2H 2 O, 1gH 3 BO 3 , 0.2g CoCl 2 ·6H 2 O), pH 7.0~7.5) in a 250mL Erlenmeyer flask, placed in a shaker at 30°C and 150rpm for 20~24h, so that the bacteria grew to the late logarithmic period, and the bacterial suspension was used for inoculation.

[0045] Take 5mL of the above bacterial suspension and add it into a sterilized 250mL Erlenmeyer flask containing 100mL of the test medium (the composition is the same as the above medium), place it in a shaker at 30°...

Embodiment 3

[0049] Embodiment 3: Denitrification and phosphorus removal ability determination of Pseudomonas aeruginosa under anoxic conditions

[0050] Inoculate the strains stored in glycerol at -20°C into 100 mL of sterilized medium (each L contains 5.0 g sodium succinate, 0.607 g NaNO 3 , 0.110g K 2 HPO 4 ·3H 2 O, 0.10g MgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, 0.02g CaCl 2 2H 2 O, 0.10mL trace element mother solution (each L contains 3.5g EDTA, 2g ZnSO 4 ·7H 2 O, 1gCuSO 4 ·5H 2 O, 1gMnSO 4 ·7H 2 O, 1gNa 2 MoO 4 2H 2 O, 1gH 3 BO 3 , 0.2g CoCl 2 ·6H 2 O), pH 7.0~7.5) in a 250mL Erlenmeyer flask, placed in a shaker at 30°C and 150rpm for 20~24h, so that the bacteria grew to the late logarithmic period, and the bacterial suspension was used for inoculation.

[0051] Take 5mL of the above bacterial suspension and add it into a sterilized 250mL Erlenmeyer flask containing 100mL of the test medium (the composition is the same as the above-mentioned pre-culture medium), pla...

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Abstract

The invention relates to a pseudomonas aeruginosa with heterotrophic nitrification-aerobic denitrification and dephosphorization functions under different dissolved oxygen conditions and application of the pseudomonas aeruginosa. The bacterial strain can carry out growth metabolism by using organic carbon as a unique carbon source and ammonia nitrogen as a unique nitrogen source, the ammonia nitrogen can be respectively converted into gas products through the heterotrophic nitrification-aerobic denitrification effect under different dissolved oxygen conditions such as the oxygen deficit condition, the microaerobic condition and the aerobiotic condition, and the goal of denitrification is reached. The bacterial strain can also carry out growth metabolism by using nitrate nitrogen as a unique nitrogen source, the nitrate nitrogen can be converted into gaseous products through the dephosphorization effect under the oxygen deficit, microaerobic and aerobiotic conditions, and the goal of denitrification is reached. Meanwhile, the bacterial strain can also reach the goal of removing phosphate in water through converting inorganic phosphorus into self components under different dissolvedoxygen conditions. Therefore, the bacterial strain can be used for synchronously removing elements such as carbon, nitrogen and phosphorus in the water under different dissolved oxygen conditions, and wide application prospects are realized in the wastewater treatment.

Description

technical field [0001] The invention relates to a strain of Pseudomonas aeruginosa and its application in wastewater biological nitrogen and phosphorus removal. The strain can exert the heterotrophic nitrification-aerobic denitrification function under different dissolved oxygen concentrations, and complete the removal of phosphate while denitrification, so as to achieve the purpose of synchronous denitrification and phosphorus removal of wastewater. Background technique [0002] At present, the massive discharge of industrial sewage and domestic wastewater has severely damaged the natural and ecological environment of our country, and also poses a great threat to human life and health. Pollutants in wastewater mainly include organic matter, heavy metals, and nitrogen and phosphorus nutrients, among which a large amount of nitrogen and phosphorus elements in the water body will induce eutrophication, which will lead to serious algal blooms, black and odorous water bodies, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/385
Inventor 倪晋仁郑茂盛仝国平
Owner PEKING UNIV
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