36 results about "Sepia esculenta" patented technology

The invention discloses a breeding method for a sepia esculenta of quick-growing strain, which belongs to the technical field of breeding aquatic fingerlings, and comprises the following steps: basic populations building, mating plan making, brood stock domesticating and strengthening cultivation, brood stock mating, eggs laying, fingerlings cultivating, fingerlings developing, fingerlings selecting, brood stock selecting and remaining, and next-generation breeding plan making. According to the method, by means of hybridizing between populations, the method takes full advantage of the hybridization to allow excellent characters of the sepia esculenta to be expressed, and meanwhile utilizes autocopuation in a population without controlling inbreeding coefficient to allow excellent genes with same hereditary basis to be subjected to homozygosis; through measurements and analyses for growing indexes in the sepia esculenta breeding process, and a male and a female with equivalent brood stock growth value can mate to conduct lay eggs for the next generation; and the sepia esculenta quick-growing character is bred in a plurality of generations to enable excellent characters of different populations to gather, so as to breed the sepia esculenta of quick-growing strain.

The invention discloses an aquatic animal attracting device which comprises a floater, a string, a soft fluorescent plastic clamping strip 1 and a soft fluorescent plastic clamping strip 2, wherein the floater is a plastic round ball with a tying lug ring; the center of the soft fluorescent plastic clamping strip 1 is provided with at least two longitudinally and uniformly arranged clamping bulges; the soft fluorescent plastic clamping strip 1 and the soft fluorescent plastic clamping strip 2 are made of the same materials and have the same size; the center of the soft fluorescent plastic clamping strip 2 is provided with longitudinally arranged clamping holes tightly matched with the clamping bulges on the soft fluorescent plastic clamping strip 1; the clamping bulges and the clamping holes are tightly combined to form lock catches; a gap is formed between the two lock catches and can be used for clamping bait; the soft fluorescent plastic clamping strip 1 and the soft fluorescent plastic clamping strip 2 are provided with small holes with same sizes in same positions; one end of the string is tied after passing through the small holes on the soft fluorescent plastic clamping strip 1 and the soft fluorescent plastic clamping strip 2; and the other end of the string passes through the tying lug ring on the floater for tying. The aquatic animal attracting device can help a 90-day sepia esculenta complete transformation from living bait to ice and fresh bait for 15-20 days. Steps comprise assembly of the aquatic animal attracting device, light irradiation and application, wherein the attracting transformation success rate reaches 90%.

The invention provides a method for harvesting and temporarily culturing sepia esculenta in a cuttlefish-shrimp polyculture pond. The method comprises steps: selecting a common ground cage for capturing prawns and a grading catching ground cage used for a prawn-crab polyculture pond; using the grading catching ground cage to catch prawns, and then using the common ground cage to capture cuttlefishes; after the cuttlefishes are caught, oxygenating and transporting the cuttlefishes with water; preparing a buffer pond and a temporary culturing pond; putting the cuttlefishes transported with water in the buffer pond to adapt for 2-3 hours, and transferring the cuttlefishes with water to the temporary culturing pond to temporarily culture; in temporary culturing, covering a blackout cloth on the temporary culturing pond, in the first week of temporary culturing, feeding fresh and alive crablats, in the second week, feeding fresh and alive palaemon carinicauda, after two weeks, feeding iced fresh small trashes; after 20-30 days of temporary culturing, using the sepia esculenta for live selling. The survival rate of the sepia esculenta after temporary culturing can reach higher than 90%. The method completely realizes live selling of the sepia esculenta, and improves economic benefits of the sepia esculenta cultivation.

An artificial breeding method of sepia esculenta belongs to the technical field of mariculture. The method is characterized by comprising the following steps: 1, selection and matching of parents; 2, strengthened culturing of the parents; 3, spawning and hatching; 4, cultivation of larvae; and 5, cultivation of young sepia esculenta. The artificial breeding method of sepia esculenta is simple in step and high in operability; the feeding amount of the sepia esculenta parents is regulated and controlled manually, particularly the sepia esculenta parents are mainly fed with fly maggots, wherein the fly maggot powder has high protein content being 56%-63%, fat content being 13%, ash content being 7%, sugar content being 3.1%, crude protein content being 15% and crude fat being 5.8%, so nutritional requirement of growth of the sepia esculenta parents can be met and thus a batch of high-quality germ cells are obtained; and then the feeding amount and the like of offspring seeds of sepia esculenta are regulated and controlled manually, and especially bait contains cuttlefish visceral meal, so the growth speed of offspring seeds of sepia esculenta can be increased and the offspring seeds are stably supplied in batches.

A method for preparing a chromosome specimen with a sepia esculenta embryonic cell, which includes the operating procedures of stripping, pre-treating, carrying out hypotonic treatment, fixing, dissociating, dripping, dyeing and washing, and is characterized in that the dripping adopts thermal dripping at the temperature ranging from 40 DEG C to 60 DEG C, the height in the dripping ranges from 1.8mto 2m, and absorbent paper is used for absorbing redundant liquid at a middle position of each liquid drop after each liquid drop is dripped. By means of the thermal dripping, the temperature of thedripping and the height in dripping are reasonably controlled, the chromosome production time is shortened, the chromosome production effect is improved, and the problems of difficulty in mastering of cell rupture degree, chromosome loss, poor chromosome morphology and the like when the chromosome specimen is prepared are solved. The method is used for the chromosome production with the sepia esculenta embryonic cell, so that the good chromosome specimen is obtained, and the chromosome is multiple in phases in metakinesis, clear in morphology and fine in dispersion. Specific effects are indicated in a figure 1.