36 results about "Sepia esculenta" patented technology

The invention discloses an aquatic animal attracting device which comprises a floater, a string, a soft fluorescent plastic clamping strip 1 and a soft fluorescent plastic clamping strip 2, wherein the floater is a plastic round ball with a tying lug ring; the center of the soft fluorescent plastic clamping strip 1 is provided with at least two longitudinally and uniformly arranged clamping bulges; the soft fluorescent plastic clamping strip 1 and the soft fluorescent plastic clamping strip 2 are made of the same materials and have the same size; the center of the soft fluorescent plastic clamping strip 2 is provided with longitudinally arranged clamping holes tightly matched with the clamping bulges on the soft fluorescent plastic clamping strip 1; the clamping bulges and the clamping holes are tightly combined to form lock catches; a gap is formed between the two lock catches and can be used for clamping bait; the soft fluorescent plastic clamping strip 1 and the soft fluorescent plastic clamping strip 2 are provided with small holes with same sizes in same positions; one end of the string is tied after passing through the small holes on the soft fluorescent plastic clamping strip 1 and the soft fluorescent plastic clamping strip 2; and the other end of the string passes through the tying lug ring on the floater for tying. The aquatic animal attracting device can help a 90-day sepia esculenta complete transformation from living bait to ice and fresh bait for 15-20 days. Steps comprise assembly of the aquatic animal attracting device, light irradiation and application, wherein the attracting transformation success rate reaches 90%.

The invention discloses a Sepia esculenta protein antioxidant peptide, and a preparation method and a use thereof. The amino acid sequence of the antioxidant peptide is Ala-Pro-Pro-Glu-Asn-Gly-Met-Ala-Gln-Met (APPENGMAQM), and the molecular weight of the antioxidant peptide is 1045.21Da. The preparation method has the advantages of science, reasonability, and easy monitoring of an enzymatic hydrolysis process, and the prepared antioxidant peptide has the advantages of strong anti-oxidation activity, easy digestion and absorption and the like, and can be used as a medicine, a healthcare food and a food additive.

The invention discloses a breeding method for a sepia esculenta of quick-growing strain, which belongs to the technical field of breeding aquatic fingerlings, and comprises the following steps: basic populations building, mating plan making, brood stock domesticating and strengthening cultivation, brood stock mating, eggs laying, fingerlings cultivating, fingerlings developing, fingerlings selecting, brood stock selecting and remaining, and next-generation breeding plan making. According to the method, by means of hybridizing between populations, the method takes full advantage of the hybridization to allow excellent characters of the sepia esculenta to be expressed, and meanwhile utilizes autocopuation in a population without controlling inbreeding coefficient to allow excellent genes with same hereditary basis to be subjected to homozygosis; through measurements and analyses for growing indexes in the sepia esculenta breeding process, and a male and a female with equivalent brood stock growth value can mate to conduct lay eggs for the next generation; and the sepia esculenta quick-growing character is bred in a plurality of generations to enable excellent characters of different populations to gather, so as to breed the sepia esculenta of quick-growing strain.

The invention relates to a long-distance transportation method of live sepia esculenta. The method includes the following steps of selecting parent , temporary rearing before transportation, stopping feeding for 24 hours, and preparing water for transportation; according to the step of temporary rearing before transportation, temperatures of the water in a temporary rearing pool is gradually transited to 16-18 DEG C, and the difference between the salinity of the water in the temporary rearing pool and the salinity of cultivation water is controlled within 2; according to the step of preparing the water for transportation, water for temporary rearing is used as the water for transportation, the temperatures of the water for transportation are reduced to 12-14 DEG C, pure oxygen is fed into the water for transportation, the live sepia esculenta is taken out of the pool, bagged and loaded in a foam box for transportation, the box is sealed and loaded to a vehicle, and temperature transition is performed when the live sepia esculenta arrives at the destination. According to the method, defects that the sepia esculenta is prone to jet ink due to severe rocking of water in the long-distance transportation of the live sepia esculenta and extremely prone to die due to high oxygen consumption rates and oxygen deficit in transportation are overcome, costs for long-distance transportation of the live sepia esculenta are reasonable, the method is easy to master, the survival rate of the long-distance transportation live sepia esculenta is over 95%, and the method has large market promotion values and can produce good economical benefits.

An artificial breeding method of sepia esculenta belongs to the technical field of mariculture. The method is characterized by comprising the following steps: 1, selection and matching of parents; 2, strengthened culturing of the parents; 3, spawning and hatching; 4, cultivation of larvae; and 5, cultivation of young sepia esculenta. The artificial breeding method of sepia esculenta is simple in step and high in operability; the feeding amount of the sepia esculenta parents is regulated and controlled manually, particularly the sepia esculenta parents are mainly fed with fly maggots, wherein the fly maggot powder has high protein content being 56%-63%, fat content being 13%, ash content being 7%, sugar content being 3.1%, crude protein content being 15% and crude fat being 5.8%, so nutritional requirement of growth of the sepia esculenta parents can be met and thus a batch of high-quality germ cells are obtained; and then the feeding amount and the like of offspring seeds of sepia esculenta are regulated and controlled manually, and especially bait contains cuttlefish visceral meal, so the growth speed of offspring seeds of sepia esculenta can be increased and the offspring seeds are stably supplied in batches.

The invention relates to artificial compound feed formula for sepia esculenta cultivation and a feed. The artificial compound feed formula for sepia esculenta cultivation comprises the components of shrimp meal, fish meal, bean pulp, peanut pulp, seaweed meal, feed yeasts, starches, fish oil, choline chloride, biological enzymes, lecithin, compound vitamin additives and compound mineral substance additives. The artificial compound feed formula disclosed by the invention provides the nutrients, namely proteins, fats, carbohydrates, trace elements, vitamins and other modifying agents and the like through multiple feed sources aiming at the nutritional requirements of sepia esculenta, ensures that nutrition proportion is balanced and can meet the nutritional requirements of the normal growth of the sepia esculenta; the feed disclosed by the invention is in the shape of a thin strip and simulated into the shapes of live baits and chilled fresh baits, thereby ensuring that young sepia esculenta is easy to transform from eating the chilled fresh baits to eat the artificial compound feed; according to the invention, the cultivation cost spent by feeding the feed produced through the artificial compound feed formula disclosed by the invention is reduced by 40% compared with the cultivation cost spent by feeding the live baits, and the survival rate is enhanced by 30% compared with the survival rate achieved by feeding the chilled fresh baits.

The invention claims a feed for culturing sepia esculenta larvae, and belongs to the technical field of aquaculture. The sepia esculenta larvae breeding feed comprises following components according to the weight percentage: 25 to 35 parts of white fish meal, 12 to 20 parts of pomfret meal, 8 to 16 parts of gulfweed meal, 10 to 25 parts of shrimp head meal, 20 to 30 parts of fermented soybean meal, 20 to 30 parts of plant starch, 2 to 8 parts of hyperglobulinemia meal, 2 to 8 parts of rice bran polysaccharide, 5 to 10 parts of calcium dihydrogen phosphate, 5 to 15 parts of choline, 1 to 5 parts of complex vitamin and 0.01 to 0.5 part of fucoidan. The feed provided by the invention has advantages of reasonable formula, comprehensive nutrition, good palatability, good stability in water, mass production and convenience in storage, transportation and feeding, thereby being suitable for industrial production of the sepia esculenta larvae. In addition, the growth rate and the survival rate of the sepia esculenta larvae are improved by adopting the feed. Therefore, the feed is suitable for large-scale popularization.

The invention discloses a cross-shaped foldable sepia esculenta egg laying reef, which comprises a cable and a cross-shaped egg laying reef on the cable, wherein a floating ball and a cement lump are arranged at the two ends of the cable respectively; the cross-shaped egg laying reef comprises two rectangular tube frameworks and a fixed middle shaft; the tube frameworks are coated with nets respectively; holes are formed in the middle parts of two wider edges of the tube frameworks; the fixed middle shaft passes through the holes to connect the two tube frameworks together, thereby forming a cross shape; the bottom end of the fixed middle shaft is provided with a circular tray, and the top end of the fixed middle shaft is buckled with an annular fixing buckle; the tube frameworks are vertically tied on the cable through a tether after being assembled into the cross shape with the fixing buckle through the fixed middle shaft. The cross-shaped foldable sepia esculenta egg laying reef is easy to assemble and disassemble, is convenient to transport and store, is low in manufacturing cost, and is durable; even if a certain part is damaged, the entire egg laying reef does not need to be discarded; the egg laying reef has small incident flow resistance and a large hatching area, and is suitable for near-shore shallow sea areas with small wind waves.

The invention provides a method for harvesting and temporarily culturing sepia esculenta in a cuttlefish-shrimp polyculture pond. The method comprises steps: selecting a common ground cage for capturing prawns and a grading catching ground cage used for a prawn-crab polyculture pond; using the grading catching ground cage to catch prawns, and then using the common ground cage to capture cuttlefishes; after the cuttlefishes are caught, oxygenating and transporting the cuttlefishes with water; preparing a buffer pond and a temporary culturing pond; putting the cuttlefishes transported with water in the buffer pond to adapt for 2-3 hours, and transferring the cuttlefishes with water to the temporary culturing pond to temporarily culture; in temporary culturing, covering a blackout cloth on the temporary culturing pond, in the first week of temporary culturing, feeding fresh and alive crablats, in the second week, feeding fresh and alive palaemon carinicauda, after two weeks, feeding iced fresh small trashes; after 20-30 days of temporary culturing, using the sepia esculenta for live selling. The survival rate of the sepia esculenta after temporary culturing can reach higher than 90%. The method completely realizes live selling of the sepia esculenta, and improves economic benefits of the sepia esculenta cultivation.

The invention discloses a method for mixedly breeding sea cucumber and sepia esculenta in a pond. The method includes the steps of pond selecting, pond transforming, pond fertilizer and water preparation, young sea cucumber releasing, feed feeding, breeding management, sepia esculenta finishing and harvesting and subsequent sea cucumber breeding and harvesting. According to the method, crablat, shrimps, gobies and other enemies adverse to the sea cucumber can be caught and killed by the sepia esculenta, the utilization rate of water can be increased through mixed breeding of the sea cucumber and the sepia esculenta, and thus the sea cucumber and the sepia esculenta are bred in a mixed mode; by means of the method, the effects of value adding and synergia are achieved for the sea cucumber and the sepia esculenta, the utilization rate of water is increased, and the effects of purifying water and improving the breeding environment are achieved.

The invention provides a method for parent raising and training and in-advance fry emergence of sepia esculenta. The method includes the steps of raising and training pond selection and modification,hatching pond selection and modification, palaemon carinicauda autonomous breeding, parent selection and stocking, ovum collecting, fertilized ovum temporary raising and concentrated hatching, opossumshrimp autonomous breeding and young sepia esculenta culture. An shrimp and crab fry culturing workshop and an available breeding earthen pond in autumn and winter are sufficiently utilized, the source of parents during artificial culturing of sepia esculenta is limited, the ecological complementation between the parents of sepia esculenta and palaemon carinicauda, young sepia esculenta and opossum shrimps is used for conducting parent raising and training and young sepia esculenta culturing, a hatching pond can meet the demand of fertilized ovum hatching for water quality, and technical operation is easy. The breeding and growing capacity of the palaemon carinicauda and opossum shrimps is quite high, continuous breeding can be realized in the whole year, the demand of the parents of thesepia esculenta and the young sepia esculenta for live baits is met, the targets of high yield, high efficiency and environmental protection are realized, and sufficient fry can be provided when breeding is conducted in the sepia esculenta pond.

The invention belongs to aquaculture industries, and particularly relates to a sepia esculenta attaching substratum used when sepia esculenta spawns. The sepia esculenta attaching substratum is composed of an upper beam and a lower beam, and is characterized in that vertical rods are installed between the upper beam and the lower beam, and nylon ropes are installed on the upper sides of the vertical rods respectively; except for the nylon ropes, other parts are made of plastics or stainless steel materials. The sepia esculenta attaching substratum has the advantages that the nylon ropes are installed on the upper sides of the vertical rods so that sepia esculenta spawns can be attached to the upper sides of the nylon ropes, and the survival rate of the sepia esculenta spawns can be greatlyimproved, and the economic benefits are improved. In a word, the sepia esculenta attaching substratum is convenient to use, the survival rate of the sepia esculenta spawns can be greatly improved, and the economic benefits are improved.

The invention relates to a method for quickly and high-efficiently detecting a sepia esculenta hoyle population. The method can be used for detecting the sepia esculenta hoyle population through collecting environment DNA (Deoxyribonucleic Acid) without damaging an environment and a species population. The collected eDNA samples are sampled from soil, silt, water and the like, so that an environment burden cannot be caused on a detecting point, and an adverse effect cannot be caused on a species survival environment; a special primer and a special probe are utilized for accurately, quickly andhigh-efficiently detecting DNA of sepia esculenta hoyle, so that the detection efficiency and the accuracy are improved, and the detection cost is reduced; compared with a field investigation methodutilized by traditional sepia esculenta hoyle population distribution detection, the method has the advantages of saving both time and labor, low cost, high efficiency, and no harm to target species or damaging an ecological system of a survey spot.

The invention belongs to the technical field of pharmaceutical products, and particularly relates to a composition for preventing the scabing of a wound. The composition is prepared from the following raw materials: crude pollen typhae, caulis sargentodoxae, herba clinopodii, herba rabdosiae, cortex albiziae, caulis polygoni multiflori, herba dipsacaceae, herba chinese mesona, thallus laminariae thallus eckloniae, myrrh, radix ampelopsis, cortex moutan, aloe, herba brassicaceae, rhizoma arisaematis, polygonum chinense herb, sepia esculenta, rhizoma polygontum cuspidatum, pericarpium citri reticulatae viride, spora lygodii, lespedeza cuneata, and edible cactus. By virtue of a reasonable formula proportion, the composition has a remarkable effect for preventing the scabing of the wound, can avoid the pain brought by Western medicine and physical therapy to a patient, has no side effect and toxicity after being used, is simple in operation, easy for getting the used Chinese herbs, and is free from toxicity and side effect for the human body.

The invention relates to the technical field of culture and proliferation of aquatic products, in particular to a sepia esculenta fry proliferation method. The method is characterized by comprising following steps: 1), selection of sea regions: a sea area with seawater meeting the first-class seawater standard is selected as a fry proliferation region, and a sea area meeting the second-class seawater standard is selected as an alternative proliferation region; 2), fry breeding: sepia esculenta which is obtained by sea fishing or bred artificially is taken as a parent, when fertilized eggs areproduced by the parent, egg attached bases are transferred out and put in a hatching pond, and the hatching pond is continuously taken as a juvenile sepia esculenta culture pond after juvenile sepia esculenta is hatched out; 3), discharging of fries out of the pond: a fry discharge box is customized and put in a foam box, and the sepia esculenta fries are sucked into the fry discharge box; 4), proliferation on the sea: fry bags are loaded on a ship and thrown dispersedly; 5), recapturing on the sea: the immature sepia esculenta is fished as a commodity for processing and sale, and sexually mature male or female individuals are fished and serve as parents for fry culture. The method has the advantage of integrating protection and utilization.

The invention relates to a technique of sibling species identification, and in particular to a method for sepia sibling species identification. Specifically, the method comprises the following steps:with sepia genome DNA as a template, designing a primer in accordance with a tag sequence of the sepia species genome; and on the basis of Tm value difference of an amplification value in a high-resolution melting curve (HRM), distinguishing sepia species. With the application of the method provided by the invention, species identification can be implemented rapidly, economically and conveniently;and the accuracy rate of the method, in comparison with a tag sequence sequencing result, is 100%.