Sepia esculenta protein antioxidant peptide, and preparation method and use thereof

A technology of antioxidant peptide and golden squid, applied in the field of engineering, can solve the problems of unreported application, and achieve the effect of scientific and reasonable process, high activity and easy monitoring

Inactive Publication Date: 2013-12-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the applicant found that using golden squid protein as a raw material, using techniques such as enzymatic hydrolysis,

Method used

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  • Sepia esculenta protein antioxidant peptide, and preparation method and use thereof
  • Sepia esculenta protein antioxidant peptide, and preparation method and use thereof
  • Sepia esculenta protein antioxidant peptide, and preparation method and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1) Homogenize the washed and shelled golden squid meat with a high-speed tissue masher, then add isopropanol according to the ratio of solid to liquid 1g:4mL, degrease at 35°C for 24 h, and then centrifuge at 4500 rpm for 15 min to remove isopropanol. Propanol, collect defatted golden cuttlefish meat solids;

[0032] 2) Add 0.2 mol / L phosphate buffer solution to the above-mentioned defatted golden squid meat solid according to the solid-to-liquid ratio of 1g:3mL, and adjust the pH to 7.0 to obtain a mixed solution;

[0033] 3) Raise the temperature of the mixture to 55°C, stir and preheat for 10 minutes, add 1.0% papain based on the mass of the defatted golden cuttlefish meat solids, and enzymolyze it at 55°C for 5 hours to obtain the enzymatic hydrolysis product;

[0034] 4) Heat the above enzymatic hydrolyzate to 90°C and keep it at a constant temperature for 15 minutes, then cool to room temperature, then centrifuge at 4500 rpm for 15 minutes to obtain the enzymatic ...

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PUM

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Abstract

The invention discloses a Sepia esculenta protein antioxidant peptide, and a preparation method and a use thereof. The amino acid sequence of the antioxidant peptide is Ala-Pro-Pro-Glu-Asn-Gly-Met-Ala-Gln-Met (APPENGMAQM), and the molecular weight of the antioxidant peptide is 1045.21Da. The preparation method has the advantages of science, reasonability, and easy monitoring of an enzymatic hydrolysis process, and the prepared antioxidant peptide has the advantages of strong anti-oxidation activity, easy digestion and absorption and the like, and can be used as a medicine, a healthcare food and a food additive.

Description

technical field [0001] The invention belongs to the field of engineering technology, in particular to functional food processing technology or biopharmaceutical technology, in particular to a method for enzymatically hydrolyzing golden squid protein to prepare antioxidant peptides. Background technique [0002] Free radicals produced during human metabolism and food oxidative deterioration can cause severe oxidative damage to cells and tissues, leading to diseases such as cardiovascular disease, arteriosclerosis, cancer and body aging. Chemically synthesized antioxidants represented by butylhydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tert-butylhydroquinone (TBHQ) are widely used in food due to their good antioxidant activity and low price. in industry. However, the toxic and side effects of chemically synthesized antioxidants have aroused widespread concern, and some countries have limited or banned their use. Therefore, natural antioxidants have become a hot ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/16A61K38/08A61P39/06A23L1/29A23L1/305A23L33/18
Inventor 王斌郁迪徐银峰胡发远
Owner ZHEJIANG OCEAN UNIV
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